作者： Hu, Xiaoling;Liu, Yiming;Wu, Junting;Liu, Yu;Liu, Wenjie;...

期刊： Brain, Behavior, and Immunity,2020年88:507-514 ISSN：0889-1591

通讯作者： Yang, Fengrui

作者机构： [Hu, Xiaoling; Liu, Yu; Wu, Junting; Yang, Fengrui; Liu, Wenjie] Univ South China, Affiliated Hosp 1, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.;[Liu, Yiming] Univ South China, Affiliated Nanhua Hosp, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.;[Chen, Ji] Univ South China, Affiliated Hosp 1, Dept Endocrinol, Hengyang 421001, Hunan, Peoples R China.;[Yang, Fengrui] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21287 USA.

通讯机构： [Yang, Fengrui] U;Univ South China, Affiliated Hosp 1, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.

关键词： P2X7 receptor (P2X7R);Amygdala;Neuropathic pain;Microglia;Astrocytes

摘要： The amygdala circuitry and P2X7 receptor (P2X7R) have both been shown to play important roles in the modulation of neuropathic pain (NP). However, little is known about the functional role of P2X7R in the amygdala for the regulation of NP. This study aims to evaluate the alleviative effect of intra-amygdala microinfusion of a pharmacological antagonist of P2X7R (A-438079) on NP and explore its possible mechanism of action. Male Sprague-Dawley rats were used to construct the animal model of NP through spared nerve injury (SNI). The SNI rats randomly received chronic bilateral microinjection of A-438079 (100 pmol/side) or saline into the amygdalae via cannulas. Mechanical paw withdrawal threshold (MWT) and thermal withdrawal duration (TWD) were measured by von Frey monofilaments. Besides, tail suspension test (TST), forced swimming test (FST), open field test (OFT) and sucrose preference test (SPT) were performed to assess depression- and anxiety-like behaviors. Immunofluorescence assay was employed to determine the levels of glial fibrillary acidic protein (GFAP), ionized calcium binding adaptor molecule 1 (IBA-1) and connexin 43 (Cx43) in the spinal cord. In addition, the change of growth associated protein 43 (GAP43) level in the spinal cord was assessed by Western blot. Our data showed that chronic treatment with A-438079 increased MWT and decreased TWD on days 11–21 post-SNI while decreased depression-like and anxiety-like behaviors. A-438079 administration significantly attenuated the elevated immunoreactivities of IBA-1 and GFAP in microglia and astrocytes after SNI. Furthermore, the decreased expression of GAP-43 in the spinal cord due to SNI was significantly attenuated by A-438079. However, when A-438079 and a pharmacological agonist (BzATP) of P2X7R were given simultaneously, all the effects caused by A-438079 alone were reversed. In brief, our study revealed the protective role of inhibiting P2X7R in the amygdala against symptoms associated with NP, possibly attributing to its inhibitory effects on spinal microglia and astrocytes. © 2020 The Authors

语种： 英文

作者： 陈聪;沈文涛;刘彦

期刊： 中国病原生物学杂志,2020年15(02):241-245 ISSN：1673-5234

作者机构： 南华大学衡阳医学院病原生物学研究所人体寄生虫学教研室,湖南衡阳421001;[沈文涛] 湖南省黄州市团风县回龙山镇卫生院;[刘彦; 陈聪] 南华大学

关键词： 血吸虫;肝纤维化;巨噬细胞;炎性免疫;微环境;综述

摘要： 血吸虫性肝纤维化是一种由血吸虫虫卵在肝脏中沉积引起的慢性免疫性疾病。因其感染症状的非特异性,常易被忽视而导致发生肝脾肿大、肉芽肿、门静脉高压和肝纤维化。临床上主要是通过药物吡喹酮杀死成虫,尚无有效针对肝纤维化的防治策略。研究发现,巨噬细胞极化在血吸虫性肝纤维化发展进程中发挥着重要作用,但其机制尚不明晰。本文通过综述巨噬细胞在肝纤维化中的研究进展,为血吸虫性肝纤维化的机制研究提供新的思路和方向。

语种： 中文

作者： Huang, Wenhui;Huang, Cheng;Ding, Huanyu;Luo, Jianfang;Liu, Yuan;...

期刊： Journal of Clinical Laboratory Analysis,2020年34(1):e23028- ISSN：0887-8013

通讯作者： Jiang, Zhisheng

作者机构： [Huang, Wenhui; Jiang, Zhisheng] Univ South China, Hengyang Med Sch, Inst Cardiovasc Dis, Hengyang, Hunan, Peoples R China.;[Huang, Wenhui; Jiang, Zhisheng] Univ South China, Hengyang Med Sch, Key Lab Arteriosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.;[Huang, Wenhui; Liu, Yuan; Ding, Huanyu; Huang, Cheng; Luo, Jianfang] Guangdong Acad Med Sci, Guangdong Cardiovasc Inst, Vasc Ctr,Guangdong Prov Key Lab Coronary Heart Di, Dept Cardiol,Guangdong Prov Peoples Hosp, Guangzhou, Peoples R China.;[Fan, Ruixin; Xiao, Fei; Fan, Xiaoping] Guangdong Acad Med Sci, Guangdong Cardiovasc Inst, Dept Cardiovasc Surg,Guangdong Prov Peoples Hosp, Vasc Ctr,Guangdong Prov Key Lab South China Struc, Guangzhou, Peoples R China.

通讯机构： [Jiang, Zhisheng] U;Univ South China, Hengyang Med Sch, Inst Cardiovasc Dis, Hengyang, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Key Lab Arteriosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.

关键词： aortic dissection;miR-145;proliferation;SMAD3;vascular smooth muscle cell

摘要： Aim: The current study aimed to examine miR-145's contribution to thoracic aortic dissection (AD) development by modulating the biological functions of vascular smooth muscle cells (VSMCs). Methods: The concentration of circulating miR-145 was determined in patients with AD and healthy controls using quantitative polymerase chain reaction (qPCR). Aortic specimens were obtained from both individuals with Stanford type A AD undergoing surgical treatment and deceased organ donors (serving as controls) whose causes of death were nonvascular diseases. Then, qPCR and fluorescence in situ hybridization were applied to assess miR-145 amounts and location, respectively. Furthermore, qPCR and immunoblot were employed to determine SMAD3 (the target gene of miR-145, involved in the TGF-β pathway) amounts at the gene and protein levels, respectively. Moreover, in vitro transfection of VSMCs with miR-145 mimics or inhibitors was conducted. Finally, the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Transwell assay and flow cytometry were employed for detecting VSMC proliferation, migration, and apoptosis, respectively. Results: The amounts of miR-145 in plasma and aortic specimens were markedly reduced in the AD group in comparison with control values (P <.05). miR-145 was mostly located in VSMCs. Proliferation and apoptosis of VSMCs were significantly induced in vitro by the downregulation of miR-145. Also, miR-145 modulated SMAD3 expression. Conclusions: miR-145 was found to be downregulated in patients with AD, which induced the proliferation, migration, and apoptosis of VSMCs by targeting SMAD3. This suggested the involvement of miR-145 in the pathogenesis of AD. © 2019 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc.

语种： 英文

作者： Zou, Ju;Wu, Daichao*;Li, Tao;Wang, Xianwen;Liu, Yan;...

期刊： PATHOLOGY RESEARCH AND PRACTICE,2019年215(2):229-234 ISSN：0344-0338

通讯作者： Wu, Daichao;Tan, Sijie

作者机构： [Liu, Yan; Zou, Ju] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hunan Prov Key Lab Special Pathogens Prevent & Co, Dept Parasitol,Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Tan, Sijie; Wu, Daichao] Univ South China, Hengyang Med Coll, Dept Histol & Embryol, Inst Clin Anat & Reprod Med, Hengyang 421001, Hunan, Peoples R China.;[Li, Tao; Wang, Xianwen] Univ South China, Hengyang Med Coll, Grade 2015 Clin Med, Hengyang 421001, Hunan, Peoples R China.;[Wu, Daichao; Tan, SJ] Univ South China, Hengyang Med Coll, Inst Clin Anat & Reprod Med, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Wu, DC; Tan, SJ] U;Univ South China, Hengyang Med Coll, Inst Clin Anat & Reprod Med, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： Cancer;Meta-analysis;PD-L1;SNP

摘要： Programmed death ligand 1(PD-L1) mediated immune escape play important roles in the development of cancer. The gene polymorphism of PD-L1, in particular rs4143815 C > G, has been associated with the cancer risks, but with conflicting results. Therefore, this meta-analysis was aimed to assess the association between rs4143815 C > G and cancer susceptibility. A systematic literature search was performed to select the studies and the pooled odds ratio (OR) with 95% confidence interval (CI) was used to evaluate the strength of association. Eleven eligible studies containing 3711 cases and 3704 controls were enrolled in the meta-analysis. The results suggested that there is a strong association between rs4143815 C > G and the cancer risks (G vs. C: OR = 1.386, 95% CI: 1.132-1.696, p = 0.002; GG vs. CG + CC: OR = 1.843 95% CI: 1.300-2.613, p = 0.002; GG + CG vs. CC: OR = 1.280, 95% CI: 1.040-1.576, p = 0.020). Subgroup analysis based on cancer type suggested that PD-L1 rs4143815 C > G might increase the susceptibility to gastric cancer (G vs. C: OR = 1.842, 95% CI: 1.403-2.418, p < 0.001) and bladder cancer (G vs. C: OR = 2.015, 95% CI: 1.556-2.608, p < 0.001), and genotype GG carriers of PD-L1 rs4143815 C > G might have higher risks of HCC (GG vs. CG + CC: OR = 2.226 95% CI: 1.562-3.172, p < 0.001). PD-L1 rs4143815 C > G might confer an increased cancer risk, indicating this SNP may contribute to the pathogenesis of cancer and might be used as a potential biomarker to predict the susceptibility to cancer.

语种： 英文

作者： 佟娜仁其木格;邹菊;陈聪;申海艳;刘彦

期刊： 中南医学科学杂志,2019年47(2):120-124,138 ISSN：2095-1116

作者机构： 南华大学衡阳医学院病原生物学研究所,湖南省特殊病原体防治重点实验室,湖南省分子靶向新药研究合作创新中心,湖南 衡阳421001;[邹菊; 刘彦; 申海艳; 陈聪; 佟娜仁其木格] 南华大学

关键词： 日本血吸虫;多肽;巨噬细胞;碱性磷酸酶

摘要： 日本血吸虫肝硬化与宿主巨噬细胞极化密切相关,本研究为验证多肽干预宿主巨噬细胞极化的分子机制,人工培养小鼠巨噬细胞,分别用单磷酸腺苷、日本血吸虫碱性磷酸酶、外源性短肽处理,ELISA检测各试剂最佳浓度;免疫印迹法检测短肽作用后细胞内一氧化氮合酶以及精氨酸酶1蛋白含量变化;酶联免疫吸附法检测细胞上清液中腺苷浓度的改变,对硝基苯磷酸二钠法检测日本碱性磷酸酶酶活,实时荧光定量PCR法检测细胞上清液中一氧化氮合酶、精氨酸酶1 mRNA的表达变化,硝酸还原酶法测定一氧化氮的浓度,免疫荧光技术鉴别M1和M2型巨噬细胞。结果显示,单磷酸腺苷、碱性磷酸酶及短肽最佳浓度分别为2. 5 mmol/L、1. 1 mmol/L及0. 6mmol/L;经多肽刺激小鼠巨噬细胞后分泌的一氧化氮合酶的蛋白及mRNA表达均上调,精氨酸酶1表达下调,碱性磷酸酶酶活降低（P<0. 01）,腺苷受体激活率下降（P<0. 01）,M1型巨噬细胞增多,M2型巨噬细胞减少。表明多肽通过降低碱性磷酸酶酶活,减少腺苷A合成,从而促进巨噬细胞经典途径激活极化,抑制巨噬细胞替代途径激活极化。

语种： 中文

作者： Zou, Ju;Wu, Daichao;Liu, Yan;Tan, Sijie*

期刊： Gynecological Endocrinology,2019年35(1):81-85 ISSN：0951-3590

通讯作者： Tan, Sijie

作者机构： [Liu, Yan; Zou, Ju] Univ South China, Dept Parasitol, Sch Med, Hengyang, Peoples R China.;[Tan, Sijie; Wu, Daichao] Univ South China, Dept Histol & Embryol, Sch Med, Inst Clin Anat & Reprod Med, Hengyang 421001, Peoples R China.

通讯机构： [Tan, Sijie] U;Univ South China, Dept Histol & Embryol, Sch Med, Inst Clin Anat & Reprod Med, Hengyang 421001, Peoples R China.

关键词： association;gene polymorphism;LHCGR;meta-analysis;PCOS

摘要： To investigate the association between Luteinizing hormone/choriogonadotropin receptor (LHCGR) gene polymorphisms and polycystic ovary syndrome (PCOS). A systematic literature search and meta-analysis using STATA software for included studies. Fourteen case-control studies containing rs13405728, rs4539842, and rs2293275 of LHCGR gene were included, which was comprised of 11,738 PCOS cases and 35,329 controls. Results of the meta-analysis showed a significant association between PCOS and rs13405728 (for G vs. A: OR = 0.735, 95% CI = 0.699–0.773, p<.001; For GG vs. AG + AA: OR = 0.578, 95% CI = 0.436–0.767, p<.001; For GG + AG vs. AA: OR = 0.817, 95% CI = 0.741–0.901, p<.001) in Asian populations, and rs4539842 (for ins/ins vs. ins/non + non/non: OR = 0.686, 95% CI = 0.483–0.974, p=.035) and rs2293275 (for AA vs. AG + GG: OR = 4.115, 95% CI = 1.033–16.38, p=.045) in Caucasian populations, respectively. LHCGR gene variations are population specifically associated with PCOS, which indicated these SNPs in LHCGR may contribute to the pathogenesis of PCOS and could be used as potential biomarkers to predict the risk of PCOS. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： 段丽;陈艺;杨帆;杜丹;王可耕;...

期刊： 中国现代医生,2019年57(31):1-4+9 ISSN：1673-9701

作者机构： 南华大学衡阳医学院病原生物学研究所,湖南衡阳421001;湖南省郴州市第一人民医院,湖南郴州423000;湖南省郴州市第一人民医院,湖南郴州,423000;南华大学衡阳医学院病原生物学研究所,湖南衡阳,421001;南华大学护理学院,湖南衡阳,421001

关键词： 非梗阻性无精症;抗缪勒管激素;睾丸显微取精术;精子检获

摘要： 目的 探讨抗缪勒管激素(AMH)水平对非梗阻性无精子症(NOA)的临床预测价值.方法 采用酶联免疫法和电化学发光免疫分析法检测NOA患者精浆AMH、血清促卵泡激素(FSH)以及睾酮(T)的浓度;B超检测其睾丸体积;通过睾丸显微取精术(M-TESE)检获精子;同时设置对照组,并进行统计学分析.结果 NOA组的AMH浓度分别低于OA组[(19.53±9.13)pmol/L,(52.34±15.13)pmol/L,P＜0.05]及NF组[(158.53±37.45)pmol/L,P＜0.05];NOA组的FSH浓度分别高于OA组[(18.36±8.95)U/L,(5.51±3.32)U/L,P＜0.05]及NF组[(6.12±3.02)U/L,P＜0.05];NOA患者的睾酮浓度[(15.32±5.43) nmol/L]分别与OA组[(15.63±6.23) nmol/L]、NF组[(15.81 ±5.73) nmol/L]比较无显著性差异(P＞0.05,P＞0.05);NOA组左右睾丸体积TV[(9.58±3.83)mL,(7.46±3.57)mL]分别与OA组[(16.97±2.56)mL,(15.32±3.63)mL]、NF组[(16.23±3.53)mL,(16.84±2.83)mL]存在显著性差异(P＜0.05,P＜0.05).NOA组有10人检获精子,OA组19人检获精子(P＜0.05).Logistic回归分析检验发现AMH预测NOA患者睾丸内是否存在精子的拟合优度最佳(Wald x2=26.198,P＜0.01);ROC曲线图显示精浆AMH的AUC值最大;精浆AMH的阳性似然比为7.36[81.82％/(1-88.89％)],阴性似然比为0.20[(1-81.82％)/88.89％].结论 精浆AMH浓度对于预测NOA患者M-TESE成功与否具有重要的意义.

语种： 中文

作者： Chen, Hong-Tao;Liu, Hao;Mao, Min-Jie;Tan, Yuan;Mo, Xiang-Qiong;...

期刊： Molecular Cancer,2019年18(1):1-19 ISSN：1476-4598

通讯作者： Jiang, Guan-Min;Shan, Hong

作者机构： [Liu, Yan; Tan, Yuan; Jiang, Guan-Min; Chen, Hong-Tao] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Clin Lab, Zhuhai 2528000, Guangdong, Peoples R China.;[Liu, Hao] Guangzhou Med Univ, Canc Hosp, Guangzhou, Guangdong, Peoples R China.;[Liu, Hao] Guangzhou Med Univ, Canc Res Inst, Guangzhou, Guangdong, Peoples R China.;[Mao, Min-Jie] Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, Dept Lab Med, State Key Lab Oncol South China,Canc Ctr, Guangzhou, Guangdong, Peoples R China.;[Tan, Yuan] Cent S Univ, Dept Clin Lab, Hunan Canc Hosp, Affiliated Canc Hosp,Xiangya Sch Med, Changsha, Hunan, Peoples R China.

通讯机构： [Jiang, Guan-Min; Shan, Hong] S;Sun Yat Sen Univ, Affiliated Hosp 5, Dept Clin Lab, Zhuhai 2528000, Guangdong, Peoples R China.;Sun Yat Sen Univ, Guangdong Prov Key Lab Biomed Imaging, Affiliated Hosp 5, Zhuhai 2528000, Guangdong, Peoples R China.

关键词： Autophagy;Epithelial-mesenchymal transition;Cancer metastasis;Anticancer therapy

摘要： Autophagy is a highly conserved catabolic process that mediates degradation of pernicious or dysfunctional cellular components, such as invasive pathogens, senescent proteins, and organelles. It can promote or suppress tumor development, so it is a “double-edged sword” in tumors that depends on the cell and tissue types and the stages of tumor. The epithelial-mesenchymal transition (EMT) is a complex biological trans-differentiation process that allows epithelial cells to transiently obtain mesenchymal features, including motility and metastatic potential. EMT is considered as an important contributor to the invasion and metastasis of cancers. Thus, clarifying the crosstalk between autophagy and EMT will provide novel targets for cancer therapy. It was reported that EMT-related signal pathways have an impact on autophagy; conversely, autophagy activation can suppress or strengthen EMT by regulating various signaling pathways. On one hand, autophagy activation provides energy and basic nutrients for EMT during metastatic spreading, which assists cells to survive in stressful environmental and intracellular conditions. On the other hand, autophagy, acting as a cancer-suppressive function, is inclined to hinder metastasis by selectively down-regulating critical transcription factors of EMT in the early phases. Therefore, the inhibition of EMT by autophagy inhibitors or activators might be a novel strategy that provides thought and enlightenment for the treatment of cancer. In this article, we discuss in detail the role of autophagy and EMT in the development of cancers, the regulatory mechanisms between autophagy and EMT, the effects of autophagy inhibition or activation on EMT, and the potential applications in anticancer therapy.

语种： 英文

作者： Chen, Hainan;Liu, Yijian;Gui, Qingjun;Zhu, Xiao;Zeng, Lin;...

期刊： Peptides,2019年111(1):118-126 ISSN：0196-9781

通讯作者： He, Jin;Yin, Kai

作者机构： [Gao, Ling; Chen, Hainan; Yin, Kai; Feng, Juling; Jackson, Ampadu O.; Li, Yi; Gui, Qingjun; Qing, Jina; Zhu, Xiao] Univ South China, Med Sch, Res Lab Clin & Translat Med, Hengyang 421001, Peoples R China.;[Chen, Hainan; Yin, Kai; Zhu, Xiao] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Liu, Yijian] Third Hosp Changsha, Changsha 410000, Hunan, Peoples R China.;[Zeng, Lin] Univ South China, Affiliated Hosp 1, Dept Neurol, Hengyang 421001, Peoples R China.;[Meng, Jun; He, Jin] Univ South China, Affiliated Hosp 1, Funct Dept, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [He, Jin; Yin, Kai] U;Univ South China, Affiliated Hosp 1, Funct Dept, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Res Lab Clin & Translat Med, Hengyang 421001, Peoples R China.

关键词： *Acute myocardial infarction;*EndMT;*Ghrelin;*Myocardial fibrosis;*Smad7;*TGF-beta1

摘要： Ghrelin, a peptide hormone produced in the gastrointestinal tract, has recently been found to be associated with the onset of myocardial fibrosis (MF). The exact mechanism, however, remains elusive. This study sought to identify the function and mechanism of ghrelin on MF after acute myocardial infarction (AMI). AMI was established in Spraque-Dawley rats by ligation of the left anterior descending (LAD). Ghrelin or saline was intraperitoneally injected two times per day for 8 weeks after ligation. The weight of heart (mg) and the weight ratio of heart to body (mg/g) as well as the fibrotic area were increased, while serum level of ghrelin was decreased after AMI. Ghrelin significantly ameliorated MF and decreased deposition of collagens in perivascular fibrosis area. In addition, ghrelin inhibited Endothelial-to-mesenchymal transition (EndMT), a crucial process for MF, in perivascular fibrosis area and TGF-β1-induced human coronary artery endothelial cells (HCAECs). Mechanistically, ghrelin persistently decreased the phosphorylation of Smad2/3 and enhanced the expression of Smad7 and p-AMPK in vivo and in vitro. After the abolition of Smad7, GHSR-1a and AMPK pathway, the effect of ghrelin on EndMT was significantly inhibited. In conclusion, these results presented a novel finding that ghrelin attenuated MF after AMI via regulation EndMT in a GHSR-1a/AMPK/Smad7- dependent manner. © 2018 Elsevier Inc.

语种： 英文

作者： Li, Tao;Liu, Youtan;Xu, Wei;Dai, Xingui;Liu, Ruimeng;...

期刊： Laboratory Investigation,2019年99(6):819-829 ISSN：0023-6837

通讯作者： Chen, Zhongqing;Li, Yunfeng

作者机构： [Li, Tao; Chen, Zhongqing; Xu, Wei] Southern Med Univ, Sch Clin Med 1, Nanfang Hosp, Dept Crit Care Med, Guangzhou 510515, Guangdong, Peoples R China.;[Li, Tao; Li, Yunfeng; Dai, Xingui] Univ South China, Peoples Hosp Chenzhou 1, Dept Crit Care Med, Inst Translat Med, Chenzhou 423000, Peoples R China.;[Liu, Youtan; Liu, Ruimeng] Southern Med Univ, Shenzhen Hosp, Dept Anesthesiol, Shenzhen 518110, Peoples R China.;[Gao, Youguang] Fujian Med Univ, Affiliated Hosp 1, Dept Anesthesiol, Sch Clin Med 1, Fuzhou 350005, Fujian, Peoples R China.

通讯机构： [Chen, Zhongqing] S;[Li, Yunfeng] U;Southern Med Univ, Sch Clin Med 1, Nanfang Hosp, Dept Crit Care Med, Guangzhou 510515, Guangdong, Peoples R China.;Univ South China, Peoples Hosp Chenzhou 1, Dept Crit Care Med, Inst Translat Med, Chenzhou 423000, Peoples R China.

摘要： Mitophagy removes dysfunctional mitochondria and is known to play an important role in the pathogenesis of several diseases; however, the role of mitophagy in acute respiratory distress syndrome (ARDS) remains poorly understood. While we have previously demonstrated that polydatin (PD) improves lipopolysaccharide (LPS)-induced ARDS, the specific mechanism remains unclear. In present study, we aimed to determine whether PD activates Parkin-dependent mitophagy to protect against LPS-induced mitochondria-dependent apoptosis and lung injury. To establish the ARDS model, C57BL/6 mice were intratracheally injected with LPS (5 mg/kg) in vivo and Beas-2B cells were exposured to 0.5 mM LPS in vitro. Our results indicate that PD facilitates Parkin translocation to mitochondria and promotes mitophagy in ARDS-challenged mice and LPS-treated Beas-2B cells. However, PD-induced mitophagy was suppressed in Parkin-/- mice and Parkin siRNA transfected cells, indicating that PD activates Parkin-dependent mitophagy. Furthermore, the protective effects of PD against LPS-induced mitochondria-dependent apoptosis and lung injury were suppressed when Parkin was depleted both in vivo and in vitro. The inhibition of mitophagy with mitophagy inhibitor mitochondrial division inhibitor-1 in vivo and silencing of autophagy-related gene 7 in vitro also blocked the protective effects mediated by PD. Our data suggest that Parkin-dependent mitophagy induced by PD provides protection against mitochondria-dependent apoptosis in ARDS.

语种： 英文

作者： Tu, Bo-Xuan;Wang, Lai-Fa;Zhong, Xiao-Lin;Hu, Zhao-Lan;Cao, Wen-Yu;...

期刊： Brain Research Bulletin,2019年149:184-193 ISSN：0361-9230

通讯作者： Li, Fang;Li, Chang-Qi

作者机构： [Liu, Yu; Wang, Lai-Fa; Li, Chang-Qi; Li, Fang; Cui, Yan-Hui; Zhang, Wen-Juan; Yan, Xiao-Xin; Zhou, Shi-Fen; Tu, Bo-Xuan; Su, Jing-Zhi; Zou, Guang-Jing; Li, Song-Ji] Cent South Univ, Sch Basic Med Sci, Dept Anat & Neurobiol, Tongzipo Rd 172, Changsha, Hunan, Peoples R China.;[Zhong, Xiao-Lin] Univ South China, Inst Clin Med, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.;[Hu, Zhao-Lan] Cent South Univ, Xiangya Hosp 2, Ren Min Cent Rd 139, Changsha, Hunan, Peoples R China.;[Cao, Wen-Yu] Univ South China, Lab Clin Anat, Hengyang, Hunan, Peoples R China.

通讯机构： [Li, F; Li, CQ] C;Cent South Univ, Sch Basic Med Sci, Dept Anat & Neurobiol, Tongzipo Rd 172, Changsha, Hunan, Peoples R China.

关键词： Cognitive impairment;Decision-making;Early response genes;Social competition

摘要： Stress can influence decision-making in humans from many cognitive perspectives, while the underlying neurobiological mechanism remains incompletely understood. Food-foraging is a rodent behavior involving strategic possessing of nutritional supply in social context; experimental model of this behavior could help explore the effect of stress on decision-making and the brain mechanism thereof. In the present study, the influence of stress on food-foraging behavior was assessed in rats using an open field choosing paradigm wherein food collection (standard food or sweet food) were associated with social competition (with or without a rat in the cage). Acute restraint stress (ARS) was induced by placing the rat in a plastic restrainer for 2 h before food-foraging behavioral tests, with the effect of stress also determined biochemically and immunohistochemically. Restraint stressed rats showed anxiety-like behavior and elevation of serum corticosterone (CORT) and epinephrine (EPI) relative to controls. Both restraint and control animals preferred sugared food. However, the former group tended to forage food from a cage not occupied by a conspecific rat, whereas the control rats preferred to obtain food from the cage with a social competitor. Thus, the total amount of food foraged and eaten are reduced in the restrained rats than in controls. While the restraint animals had normal social interaction with other rats, they displayed enhanced social agonistic behavior. In brain examination, ARS attenuated the increase in immunolabeling and protein levels of c-fos, p-CREB, p-ERK1/2 in the anterior cingulate cortex (ACC) observed in control animals in association with food-foraging. These results indicate that restraint stressed rats tend to forage food by taking the advantage of a less competitive opportunity. Mechanistically, this decision-making alternative appears to be mediated through a neuronal deactivation in the ACC. The current findings provide novel insights into neuronal processing of decision-making behavior under the influence of stress. © 2019 Elsevier Inc.

语种： 英文

作者： Wang, Jiamin;Zhao, Shankun;Luo, Lianmin;Liu, Yangzhou;Li, Ermao;...

期刊： Evidence-Based Complementary and Alternative Medicine,2019年2019:8494567 ISSN：1741-427X

通讯作者： Zhao, Zhigang

作者机构： [Wang, Jiamin; Liu, Yangzhou; Zhao, Shankun; Luo, Lianmin; Zhao, Zhigang; Zhu, Zhiguo] Guangzhou Med Univ, Affiliated Hosp 1, Guangdong Prov Key Lab Urol, Dept Urol & Androl,Minimally Invas Surg Ctr, Guangzhou, Guangdong, Peoples R China.;[Li, Ermao] Univ South China, Sch Med, Res Lab Clin & Translat Med, Hengyang 421001, Peoples R China.

通讯机构： [Zhao, Zhigang] G;Guangzhou Med Univ, Affiliated Hosp 1, Guangdong Prov Key Lab Urol, Dept Urol & Androl,Minimally Invas Surg Ctr, Guangzhou, Guangdong, Peoples R China.

摘要： Objective. To evaluate the therapeutic effect of Shengjing capsules on nonobstructive azoospermia (NOA) in the rat model. Methods. Twenty-five male Sprague-Dawley rats were randomly divided into five groups as follows (n=5 per group): normal group, NOA group, and three Shengjing capsule treatment groups (low-dose, medium-dose, and high-dose groups, respectively). HE staining and semen smear were performed to assess sperm quality. The expression levels of PI3K/AKT and integrin α6/β1 were measured by qRT-PCR and western blot analyses. Results. In the NOA group, almost all of the seminiferous tubules were vacuolated with a thin layer of basal compartment containing some spermatogonial stem cells. The counts of sperms in the NOA group were strongly lower than those of the normal group (P=0.0001). The expression of PI3K/AKT and integrin α6/β1 was scarcely expressed in the NOA group. All indexes mentioned above were significantly different from those of the medium-and high-dose groups (P=0.001, all). The sperm count of rats treated with Shengjing capsules was significantly higher than that of the NOA group (P=0.0001). The rats of Shengjing capsule groups had more layers of spermatogonial stem cells and spermatocytes, and some had intracavitary sperms. Conclusions. Shengjing capsules may be a promising therapeutic medicine for NOA. The underlying mechanisms might involve activating SSCs by upregulating the integrin α6/β1 expression via the PI3K/AKT pathway. © 2019 Jiamin Wang et al.

语种： 英文

作者： Liu, Yangzhou;Zhao, Shankun;Wang, Jiamin;Zhu, Zhiguo;Luo, Lianmin;...

期刊： UROLOGIA INTERNATIONALIS,2019年102(4):373-384 ISSN：0042-1138

通讯作者： Zhao, Zhigang

作者机构： [Wang, Jiamin; Liu, Yangzhou; Zhao, Shankun; Luo, Lianmin; Zhao, Zhigang; Zhu, Zhiguo; Tang, Fucai] Guangzhou Med Univ, Guangdong Prov Key Lab Urol, Dept Urol & Androl, Minimally Invas Surg Ctr,Affiliated Hosp 2, Guangzhou 510230, Guangdong, Peoples R China.;[Li, Ermao] Univ South China, Sch Med, Res Lab Clin & Translat Med, Hengyang, Peoples R China.

通讯机构： [Zhao, Zhigang] G;Guangzhou Med Univ, Guangdong Prov Key Lab Urol, Dept Urol & Androl, Minimally Invas Surg Ctr,Affiliated Hosp 2, Guangzhou 510230, Guangdong, Peoples R China.

关键词： Castration-resistant prostate cancer;Chromogranin A;Neurone-specific enolase;Neuroendocrine;Meta-analysis

摘要： Purpose: To evaluate whether serum neuroendocrine markers could effectively predict treatment outcomes in patients with metastatic castration-resistant prostate cancer (mCRPC). Methods: The PubMed, Cochrane Library and Embase databases were sought to identify eligible studies concerning serum neuroendocrine markers and the prognosis of post-Treatment mCRPC from inception to April 2018. The association between serum neuroendocrine markers, that is, chromogranin A (CgA) and neurone-specific enolase (NSE), levels and the prognosis of post-Treatment mCRPC were summarized using a random-effects model and hazard ratio (HR) with 95% CI Sensitivity analyses were conducted to assess potential bias. Results: A total of 234 participants are included in this meta-Analysis (mean age = 71.3 years) from 6 studies. The pooled results show that higher markers' levels at baseline in patients were associated with unfavorable overall survival (OS; univariate analysis: HR 3.775, 95% CI 1.469-9.698, p = 0.006; multivariate analysis: HR 3.838, 95% CI 1.774-8.304, p = 0.001), and a similar situation was observed in progression-free survival (PFS; univariate analysis: HR 2.785, 95% CI 1.315-5.898, p = 0.007; multivariate analysis: HR 1.266, 95% CI 1.017-1.577, p = 0.035). Estimates of the total effects were generally consistent in the sensitivity analysis. Publication bias was observed when performing the univariate analysis of PFS, and we have the explanation accordingly. Conclusions: The results of this pooled analysis confirm serum neuroendocrine markers could be the effective predictor of treatment outcome in patients with mCRPC. In addition, a combination of CgA and NSE is more valuable to predict worse OS. Further randomized case-control trials are required to validate this relationship. © 2018 © 2018 S. Karger AG, Basel.

语种： 英文

作者： Liu, Yanmei;Yin, Weilan;Xu, Lingqing;Zhang, Helin;Liu, Qian;...

期刊： Molecular Biotechnology,2018年60(4):245-250 ISSN：1073-6085

通讯作者： Yin, Weiguo

作者机构： [Yin, Weiguo; Liu, Qian; Liu, Yanmei; Xu, Lingqing] Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Dept Clin Lab, Qingyuan 511518, Guangdong, Peoples R China.;[Yin, Weilan] Univ South China, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;[Yin, Weilan] Univ South China, Inst Neurosci, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Helin] Shantou Univ, Coll Med, Yuebei Peoples Hosp, Dept Clin Lab, Shaoguan 512026, Guangdong, Peoples R China.

通讯机构： [Yin, Weiguo] G;Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Dept Clin Lab, Qingyuan 511518, Guangdong, Peoples R China.

关键词： Interleukin-1 receptor-related kinase-2;Retroviral vector;Gene recombination

摘要： To identify the importance of IRAK2 kinase activity in TLR-mediated signaling pathways, we constructed a retroviral vector harboring either a mouse IRAK2 gene (IRAK2-WT) or with its mutant with loss of function of its ATP-binding site (IRAK2-KD). Further, we comparatively analyzed for the gain of function and modulations in TLR-mediated signaling pathways in IRAK2 knockout (IRAK2-KO) macrophages upon introduction of the IRAK2-WT retroviral constructs. The pBS/IRAK2-KD with the ATP-binding site mutation in IRAK2 was obtained by using site-specific mutagenesis. The recombinants were identified with appropriate double digestion and sequence analysis. The recombinant vector constructs were transfected by lipofection into phoenix packaging cells. The viral vectors (10⁷&nbsp;cfu/mL) with the construct were allowed to infect IRAK2-KO macrophages. The results showed that IRAK2-WT gene overexpressed in the IRAK2-KO macrophages exhibited a modified IRAK2 expression upon LPS induction. However, the modification was absent with IRAK2-KD construct on LPS stimulation;instead, the IRAK2 protein stability was reduced considerably. The results further show that the LPS-induced effect on the stability of IRAK2 is dependent of IRAK4 stimulation.<br/> &copy;2018, Springer Science+Business Media, LLC, part of Springer Nature.

语种： 英文

作者： 贺庆芝;罗晓清;刘彦

期刊： 教育现代化,2018年5(53):28-29 ISSN：2095-8420

作者机构： 南华大学衡阳医学院,湖南 衡阳

关键词： 双一流;医学;创新型人才

摘要： "双一流"建设中的一个重要任务就是培养具有创新思维和实践能力的人才,积极深化创新人才培养模式改革,实现人才培养质的飞跃。中央在《关于深化人才发展体制机制改革的意见》（2016）中也表示要创新人才培养模式,强调要培养真正具有创新意识和创新能力的人才。我国慢慢注重创新在社会发展中的巨大作用,各类创新人才逐渐参与到国家的发展中。作为国家重要学科,近年来我国医学发展取得重大进步,但也面临着许多问题,在"双一流"建设的背景下积极响应时代"创新"主题,培养医学创新人才是我国建设创新型国家的基础性工作,是新时代高等医学院校的重要任务。

语种： 中文

作者： 刘鸿敏;陈思睿;曾砾;刘彦

期刊： 中国校医,2018年32(2):98-99 ISSN：1001-7062

作者机构： [刘鸿敏] 衡阳市一中;中国地质大学机械与电子信息学院;南华大学医学院;南华大学寄生虫学教研室;[曾砾; 刘彦] 南华大学

关键词： 华支睾吸虫;囊蚴;感染;鲤科

摘要： 目的调查湖南省祁阳县大忠桥镇麦穗鱼华支睾吸虫囊蚴感染的情况,了解该县肝吸虫流行趋势。方法取市售麦穗鱼,采用直接压片法检查华支睾吸虫囊蚴感染率,用消化法检查其感染度。结果 2013年市售麦穗鱼感染率为74.12%（63/85）;2017年感染率为22.34%（21/94）;鱼背部肌肉囊蚴检出率为40.78%（73/179）,尾部肌肉检出率为28.49%（51/179）;2013年平均感染度为（4.16±0.60）个/100g;2017年平均感染度为（3.96±0.81）个/100g。结论大忠桥镇市售麦穗鱼2013年华支睾吸虫囊蚴的感染率高于2017年。

语种： 中文

作者： Yan, Bin;Liu, Shuang*;Shi, Ying;Liu, Na;Chen, Ling;...

期刊： CELL DEATH & DISEASE,2018年9(5):490 ISSN：2041-4889

通讯作者： Liu, Shuang;Tao, Yongguang

作者机构： [Tao, Yongguang; Liu, Shuang; Muegge, Kathrin; Yan, Bin] Cent S Univ, Xiangya Hosp, Inst Med Sci, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.;[Tao, Yongguang; Liu, Shuang; Muegge, Kathrin; Liu, Xiaoli; Mao, Chao; Lai, Weiwei; Yan, Bin; Shi, Ying; Jiang, Yiqun; Xin, Xing; Liu, Yating; Liu, Na; Cao, Ya; Yang, Rui; Chen, Ling] Cent S Univ, Xiangya Hosp, Minist Educ, Key Lab Carcinogenesis & Canc Invas, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.;[Tang, Can-E; Tao, Yongguang; Muegge, Kathrin; Liu, Xiaoli; Mao, Chao; Lai, Weiwei; Yan, Bin; Shi, Ying; Jiang, Yiqun; Xin, Xing; Liu, Yating; Liu, Na; Cao, Ya; Yang, Rui; Chen, Ling] Cent S Univ, Canc Res Inst, 110 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.;[Tao, Yongguang; Muegge, Kathrin; Wang, Xiang] Cent S Univ, Xiangya Hosp 2, Dept Thorac Surg, Changsha, Hunan, Peoples R China.;[Xiao, Desheng] Cent S Univ, Xiangya Hosp, Dept Pathol, Changsha 410008, Hunan, Peoples R China.

通讯机构： [Liu, S; Tao, YG] C;Cent S Univ, Xiangya Hosp, Inst Med Sci, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.;Cent S Univ, Xiangya Hosp, Minist Educ, Key Lab Carcinogenesis & Canc Invas, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.

摘要： Most cancer patients receive radiotherapy in the course of their disease and the occurrence of radioresistance is associated with poor prognosis. The molecular pathways that drive enhanced tumorigenic potential during the development of radioresistance are poorly understood. Here, we demonstrate that aryl hydrocarbon receptor (AhR) plays a vital role in the maintenance of cancer stem-like properties. AhR promotes the cancer stem-like phenotype and drives metastasis by directly targeting the promoters of 'stemness' genes, such as the ATP-binding cassette sub-family G member 2 (ABCG2) gene. Moreover, the radioresistant sublines display high levels of oncometabolites including α-ketoglutarate, and treatment of cancer cells with α-ketoglutarate enhances their stem-like properties in an AhR activation-dependent manner. IKKα directly activates stemness-related genes through an interaction with AhR as a bone fide chromatin modifier. Thus, AhR is functionally linked with cancer stem-like properties, and it drives tumorigenesis in the occurrence of radioresistance. © 2018 The Author(s).

语种： 英文

作者： You, Baiyang;Liu, Yanbo;Chen, Jia;Huang, Xiao;Peng, Huihui;...

期刊： Cardiovascular Research,2018年114(1):188-199 ISSN：0008-6363

通讯作者： Shi, Ruizheng;Zhang, Guogang

作者机构： [Xu, Qian; Peng, Huihui; Liu, Yanbo; Zhang, Kai; Liu, Zhaoya; Huang, Xiao; Shi, Ruizheng; Zhang, Guogang; You, Baiyang] Cent S Univ, Xiangya Hosp, Dept Cardiovasc Med, Changsha 410008, Hunan, Peoples R China.;[Chen, Jia] Cent S Univ, Xiangya Nursing Sch, Dept Humanist Nursing, Changsha 410008, Hunan, Peoples R China.;[Tang, Yixin] Univ South China, Affiliated Hosp 1, Dept Cardiovasc Med, Hengyang 421001, Peoples R China.;[Li, Xiaohui] Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410078, Hunan, Peoples R China.;[Cheng, Guangjie] Univ Alabama Birmingham, Dept Med, Div Pulm Allergy & Crit Care Med, Birmingham, AL 35294 USA.

通讯机构： [Shi, RZ; Zhang, GG] C;Cent S Univ, Xiangya Hosp, Dept Cardiovasc Med, Changsha 410008, Hunan, Peoples R China.

关键词： *Hypoxia;*NF-kappaB;*Pulmonary artery smooth muscle cells;*Pulmonary vascular remodelling;*Vascular peroxidase 1

摘要： Reactive oxygen species (ROS) play essential roles in the pulmonary vascular remodelling associated with hypoxiainduced pulmonary hypertension (PH). Vascular peroxidase 1 (VPO1) is a newly identified haeme-containing peroxidase that accelerates oxidative stress development in the vasculature. This study aimed to determine the potential role of VPO1 in hypoxia-induced PH-related vascular remodelling. The vascular morphology and VPO1 expression were assessed in the pulmonary arteries of Sprague-Dawley (SD) rats. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4) and VPO1 expression and HOCl production were significantly increased in hypoxic rats, which also exhibited obvious vascular remodelling. Furthermore, a hypoxia-induced PH model was generated by exposing primary rat pulmonary artery smooth muscle cells (PASMCs) to hypoxic conditions (3% O 2 , 48 h), which significantly increased the expression of NOX4 and VPO1 and the production of HOCl. These hypoxic changes were accompanied by enhanced proliferation, apoptosis resistance, and migration. In PASMCs, hypoxia-induced changes, including effects on the expression of cell cycle regulators (cyclin B1 and cyclin D1), apoptosis-related proteins (bax, bcl-2, and cleaved caspase-3), migration promoters (matrix metalloproteinases 2 and 9), and NF-κB expression, as well as the production of HOCl, were all inhibited by silencing VPO1 with small interfering RNAs. Moreover, treatment with HOCl under hypoxic conditions upregulated NF-κB expression and enhanced proliferation, apoptosis resistance, and migration in PASMCs, whereas BAY 11-7082 (an inhibitor of NF-jB) significantly inhibited these effects. Collectively, these results demonstrate that VPO1 promotes hypoxia-induced proliferation, apoptosis resistance, and migration in PASMCs via the NOX4/VPO1/HOCl/NF-κB signalling pathway. © 2017 Author.

语种： 英文

作者： Liu, Yami;Peng, Wen;Qu, Kai;Lin, Xiaolong;Zeng, Zhaolin;...

期刊： DNA AND CELL BIOLOGY,2018年37(6):517-523 ISSN：1044-5498

通讯作者： Wang, Zuo

作者机构： [Chen, Jiaojiao; Liu, Yami; Zeng, Zhaolin; Wang, Zuo; Wei, Dangheng] Univ South China, Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Peng, Wen] Univ South China, Affiliated Hosp 1, Dept Spine Surg, Hengyang, Hunan, Peoples R China.;[Qu, Kai] Chongqing Univ, Coll Bioengn, Chongqing, Peoples R China.;[Lin, Xiaolong] Third Peoples Hosp Huizhou, Dept Pathol, Huizhou, Peoples R China.

通讯机构： [Wang, Zuo] U;Univ South China, Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.

关键词： TET2;DNA demethylation;atherosclerosis;inflammation

摘要： Atherosclerosis is the underlying cause of cardio-cerebrovascular disease. However, the mechanisms of atherosclerosis are still unclear. The modification of DNA methylation has an important role in atherosclerosis development. As a member of the Ten-eleven translocation (TET) family, TET methylcytosine dioxygenase 2 (TET2) can modify DNA methylation by catalyzing 5-methylcytosine to 5-hydroxymethylcytosine and mediate DNA demethylation. Recent findings suggest that TET2 is related to the phenotype transformation of vascular smooth muscle cells, endothelial dysfunction, and inflammation of macrophage, the key factors of atherosclerosis. Therefore, TET2 may be a potential target for atherosclerosis treatment. This review will elaborate the recent findings that suggest the role of TET2 in atherosclerosis.

语种： 英文

作者： Lin, Xiao-Long*;Liu, Mihua;Liu, Yuanbo;Hu, Huijun;Pan, Yongquan;...

期刊： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2018年41(1):129-136 ISSN：1107-3756

通讯作者： Lin, Xiao-Long

作者机构： [Hu, Huijun; Fan, Xiaojuan; Pan, Yongquan; Lin, Xiao-Long; Zou, Weiwen; Hu, Xuemei] Guangzhou Med Univ, Huizhou Peoples Hosp 3, Dept Pathol, 1 Xuebei St, Huizhou 516002, Guangdong, Peoples R China.;[Liu, Mihua] Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Liu, Yuanbo] Sixth Peoples Hosp Huizhou, Dept Neurol, Huizhou 516211, Guangdong, Peoples R China.

通讯机构： [Lin, Xiao-Long] G;Guangzhou Med Univ, Huizhou Peoples Hosp 3, Dept Pathol, 1 Xuebei St, Huizhou 516002, Guangdong, Peoples R China.

关键词： epithelial-to-mesenchymal transition;transforming growth factor 1;signal transducer and activator of transcription 3

摘要： Transforming growth factor beta1 (TGFbeta1) is a cytokine with multiple functions. TGFbeta1 significantly induces migration and invasion of liver cancer cells. However, the molecular mechanisms underlying this effect remain unclear. Epithelialtomesenchymal transition (EMT) is crucial for the development of invasion and metastasis in human cancers. The aim of the present study was to determine whether TGFbeta1induced EMT promoted migration and invasion in HepG2 cells. The underlying mechanism and the effect of EMT on HepG2 cells were also investigated. The results demonstrated that TGFbeta1 may induce EMT to promote migration and invasion of HepG2 cells, and this effect depends on activation of the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signaling pathway. JAK/STAT3 signaling is involved in human malignancies, including lung cancer, and is implicated in cell transformation, tumorigenicity, EMT and metastasis. In the present study, TGFbeta1 also activated JAK/STAT3 signaling in HepG2 cells and promoted Twist expression, but these events were abolished by treatment with the STAT3 inhibitor AG490. Additionally, Twist siRNA blocked TGFbeta1induced EMT. Thus, TGFbeta1 was shown to induce EMT, thereby promoting the migration and invasion of HepG2 cells via JAK/STAT3/Twist signaling.

语种： 英文