摘要：
This study aims to investigate the adverse effects of different types of alpha-thalassemia on pregnant women and their differences, so as to provide reference for the prevention and monitoring of perinatal complications in different types of alpha-thalassemia pregnancy. The observation group comprised 1371 singleton pregnant women with alpha-thalassemia, control group comprised 680 non-thalassemia singleton pregnancies during the same period. The thalassemia genotypes of the observation group were tallied and categorized based on their respective genotypes within the group. The hemoglobin in the experimental group was lower than control group. The incidences of electrocardiographic abnormality, abnormal liver function, hypertensive disorders, gestational diabetes mellitus and thyroid disorders during pregnancy were higher in the observation group than in the control group. The incidences of preterm labor, caesarean section and postpartum hemorrhage were all higher in the observation group than in the control group. The delivery weight and neonatal hemoglobin in the observation group were lower than those in the control group. Pregnant women with alpha-thalassemia have more pregnancy and delivery complications than those without alpha-thalassemia, they should receive more frequent and standardized prenatal examination, so as to find and correct abnormal indicators in time.
摘要：
Polycystic Ovary Syndrome (PCOS) is a prevalent endocrinopathy in reproductive-aged women, contributing to 75% of infertility cases due to ovulatory dysfunction. The condition poses significant health and psychological challenges, making the study of its pathogenesis and treatment a research priority. This study investigates the effects of Mogroside V (MV) on PCOS, focusing on its anti-inflammatory and anti-insulin resistance properties. Forty-five female Sprague–Dawley rats were divided into three groups: control, PCOS model, and MV treatment. The PCOS model was induced using a high-fat diet and letrozole. The MV treatment group was subsequently administered MV after the establishment of the PCOS model. The study monitored body mass, assessed estrous cycle changes, and measured serum hormone levels. Transcriptome sequencing and bioinformatics were used to identify differentially expressed genes related to inflammation and insulin resistance. Expression of pyroptosis and insulin resistance markers was analyzed using qRT-PCR, Western blot, and IHC. Additionally, an in vitro model assessed MV's impact on inflammation and insulin resistance. The PCOS group exhibited elevated serum testosterone (T), luteinizing hormone (LH), insulin, and fasting glucose levels, along with increased insulin resistance (HOMA-IR) and decreased estradiol (E2), which were reversed by MV treatment. Transcriptome analysis identified significant gene expression changes between groups, particularly in pathways related to NLRP3 inflammation and insulin metabolism. MV treatment normalized the expression of ovarian pyroptosis factors (NLRP3, Caspase-1, GSDMD) and inflammatory cytokines (IL-1β, IL-18). In cellular models, MV increased E2 levels, reduced LDH release, and decreased the expression of insulin resistance and pyroptosis markers. Correlation analysis showed pyroptosis factors were positively correlated with HOMA-IR and IGF1, and negatively with IGF1R and E2 levels. MV improves PCOS by reducing pyroptosis and insulin resistance, enhancing insulin sensitivity, and promoting estrogen synthesis, thereby restoring granulosa cell function and follicular development.
通讯机构：
[Gu, WL ] U;Univ South China, Sch Civil Engn, Hengyang, Peoples R China.
关键词：
Solar radiant energy;Non-uniform heat flow;Buoyancy;Secondary flow;Thermal performance
摘要：
Under the focusing characteristics of parabolic trough solar collectors, enhancing secondary flow intensity and shifting the secondary flow vortex center towards the bottom of the absorber can extend the lifespan of the absorber and improve thermal performance. This study combines the Monte Carlo Ray Tracing method (MCRT) and the Finite Volume Method (FVM) to investigate the fluid flow and heat transfer characteristics inside the absorber under non-uniform thermal boundary conditions. The effects of inlet flow rate (Vin), inlet temperature (Tin), and protrusion structures on the secondary flow vortex position are examined. Through numerical analysis, secondary flow intensity (Se), heat transfer coefficient (h), Nusselt number (Nu), and friction factor (f) are calculated. The results show that increasing the Tin significantly enhances secondary flow intensity, thereby improving heat transfer within the absorber. Specifically, when Tin increases from 400.15 to 600.15 K, Se increases by a factor of 7.87, while h increases by 98.96%. Increasing the Vin shifts the secondary flow vortex downward, enhancing heat transfer at the bottom of the absorber. For example, when Vin increases from 100 to 200 L min−1, Se remains largely unchanged, while h increases by 44.68%. Compared to semi-cylindrical protrusions, tetrahedral protrusions are more effective at suppressing the upward shift of the secondary flow vortex, reducing velocity losses caused by fluid-wall interaction, and achieving better heat transfer enhancement. Under conditions of Tin = 500.15 K and Vin = 100 L min−1, the Nu increases by 14.6% for tetrahedral protrusions and 7.3% for semi-cylindrical protrusions, while the f increases by 12.3% and 10.9%, respectively, compared to the smooth absorber.
期刊：
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES,2025年:1-11 ISSN：1913-2751
通讯作者：
Shiyu Yan
作者机构：
[Shiyu Yan; Gang Yu; Lingna Chen] Computer School, University of South China, Hengyang, China;[Jiaoxing Yang] The First Affiliated Hospital, University of South China, Hengyang, China
通讯机构：
[Shiyu Yan] C;Computer School, University of South China, Hengyang, China
关键词：
Deep Learning technique;Synergistic drug combination;Computational model;Feature extraction
摘要：
Drug combination therapy has shown improved efficacy and decreased adverse effects, making it a practical approach for conditions like cancer. However, discovering all potential synergistic drug combinations requires extensive experimentation, which can be challenging. Recent research utilizing deep learning techniques has shown promise in reducing the number of experiments and overall workload by predicting synergistic drug combinations. Therefore, developing reliable and effective computational methods for predicting these combinations is essential. This paper proposed a novel method called Drug-molecule Connect Cell (DconnC) for predicting synergistic drug combinations. DconnC leverages cellular features as nodes to establish connections between drug molecular structures, allowing the extraction of pertinent features. These features are then optimized through self-augmented contrastive learning using bidirectional recurrent neural networks (Bi-RNN) and long short-term memory (LSTM) models, ultimately predicting the drug synergy. By integrating information about the molecular structure of drugs for the extraction of cell features, DconnC uncovers the inherent connection between drug molecular structures and cellular characteristics, thus improving the accuracy of predictions. The performance of our method is evaluated using a five-fold cross validation approach, demonstrating a 35
$$\%$$
reduction in the mean square error (MSE) compared to the next-best method. Moreover, our method significantly outperformed alternative approaches in various evaluation criteria, particularly in predicting different cell lines and Loewe synergy score intervals.
摘要：
BACKGROUND: Neuronal senescence is a common pathological feature of various neurodegenerative diseases, with ferroptosis playing a significant role. This study aims to investigate the role of ErbB4 receptor activation in preventing D-Galactose (D-gal)-induced neuronal senescence. METHODS: Mice subjected to D-gal-induced aging were administered a small molecule ErbB4 receptor agonist (E4A), identified via virtual screening, melatonin, or a combination of both. Behavioral assessments were conducted to evaluate therapeutic efficacy in memory and cognitive functions. Immunofluorescence staining, western blot, and biochemical assays were primarily employed to assess changes in both senescence- and ferroptosis-related molecules in mouse hippocampal tissues in response to each treatment. Additionally, mouse hippocampal HT22 neuronal cell cultures were utilized to corroborate the in vivo findings. RESULTS: The targeted activation of ErbB4 receptor by E4A significantly ameliorated the behavioral deficits induced by D-gal in mice, demonstrating an effect comparable to that of melatonin, a natural inhibitor of in vivo senescence and ferroptosis. Both E4A and melatonin mitigated D-gal-induced aging in hippocampal neurons of mice. This was evidenced by the upregulation of Lamin B1 and the downregulation of P53, P21, P16, GFAP, and Iba-1 expression levels. Moreover, D-gal treatment markedly decreased the protein expression of the ferroptosis inhibitor Nrf2 while augmenting the expression of the ferroptosis promoter TFRC. These alterations were partially reversed by the individual administration of E4A and melatonin. In vitro studies further corroborated that D-gal treatment significantly and concurrently induced the expression of senescence markers and ferroptosis promoters. However, both E4A and melatonin were able to significantly reverse these changes. Additionally, E4A markedly ameliorated Erastin-induced ferroptosis in mouse hippocampal neuronal cells. CONLUSION: Our findings suggest that targeted activation of ErbB4 receptor may be a viable strategy for treating neuronal senescence by inhibiting ferroptosis, thereby offering a potential therapeutic avenue for senescence-associated neurodegenerative diseases.
摘要：
BACKGROUND: Delayed diagnosis of inflammatory bowel disease (IBD) is common in Europe and North America, with limited research in Asia. We aimed to investigate factors influencing delayed diagnosis of IBD in Chinese children and the impact of delayed diagnosis on growth. METHODS: This was a retrospective study. Clinical data on children with IBD were collected through electronic medical records. The diagnostic interval includes the time from symptom onset to hospital admission and admission to diagnosis. Diagnostic delay was defined as the upper quartile of the time interval from the first symptom to the diagnosis of IBD. For the effect on growth indicators, the length of follow-up was at least 3 months from diagnosis. RESULTS: This study included 222 children with IBD, predominantly with Crohn's disease (86.0%). Approximately one-quarter of children require more than 366 days to be diagnosed with IBD, primarily due to the extended interval between the onset of initial symptoms and hospital admission. Multivariate logistic regression models showed that fever was associated with a prolonged time interval from first symptom onset to admission and the odds ratio (OR) was 0.45 [95% confidence interval (CI) 0.22-0.94]. Age and bloody stools were associated with prolonged intervals from admission to diagnosis, with ORs of 0.84 (95% CI 0.77-0.92) and 0.36 (95% CI 0.14-0.94), respectively. Delayed diagnosis was associated with height at first admission and follow-up. Children with a delayed diagnosis had a 5.87-fold higher chance of growth retardation upon initial admission compared to children without a delayed diagnosis (95% CI 1.59-24.05). After 15.7 months of follow-up, this elevated risk remained (OR 3.28, 95% CI 1.00-10.50). CONCLUSION: Delayed diagnosis is common in Chinese children with IBD and is associated with persistent height impairment.
摘要：
Pharmacogenomic landscapes and related databases are important for identifying the biomarkers of drug response and toxicity. However, these data are still lacking for the Chinese population. In this study, we constructed a pharmacogenomic landscape and an associated database using whole-genome sequencing data generated by non-invasive prenatal testing in 206,640 Chinese individuals. In total, 1,577,513 variants (including 331,610 novel variants) were identified among 3,538 pharmacogenes related to 2,086 drugs. We found that the variant spectrum in the Chinese population differed among the seven major regions. Regional differences also exist among provinces in China. The average numbers of drug enzyme, transporter, and receptor variants were 258, 557, and 632, respectively. Subsequent correlation analysis indicated that the pharmacogenes affecting multiple drugs had fewer variants. Among the 16 categories of drugs, we found that nervous system, cardiovascular system, and genitourinary system/sex hormone drugs were more likely to be affected by variants of pharmacogenes. Characteristics of the variants in the enzyme, transporter, and receptor subfamilies showed specificity. To explore the clinical utility of these data, a genetic association study was conducted on 1,019 lung cancer patients. Two novel variants, AKT2 chr19:40770621 C>G and SLC19A1 chr21:46934171 A>C, were identified as novel platinum response biomarkers. Finally, a pharmacogenomic database, named the Chinese Pharmacogenomic Knowledge Base (CNPKB: http://www.cnpkb.com.cn/ ), was constructed to collect all the data. In summary, a pharmacogenomic landscape and database for the Chinese population were constructed in this study, which could support personalized Chinese medicine in the future.
Pharmacogenomic landscapes and related databases are important for identifying the biomarkers of drug response and toxicity. However, these data are still lacking for the Chinese population. In this study, we constructed a pharmacogenomic landscape and an associated database using whole-genome sequencing data generated by non-invasive prenatal testing in 206,640 Chinese individuals. In total, 1,577,513 variants (including 331,610 novel variants) were identified among 3,538 pharmacogenes related to 2,086 drugs. We found that the variant spectrum in the Chinese population differed among the seven major regions. Regional differences also exist among provinces in China. The average numbers of drug enzyme, transporter, and receptor variants were 258, 557, and 632, respectively. Subsequent correlation analysis indicated that the pharmacogenes affecting multiple drugs had fewer variants. Among the 16 categories of drugs, we found that nervous system, cardiovascular system, and genitourinary system/sex hormone drugs were more likely to be affected by variants of pharmacogenes. Characteristics of the variants in the enzyme, transporter, and receptor subfamilies showed specificity. To explore the clinical utility of these data, a genetic association study was conducted on 1,019 lung cancer patients. Two novel variants, AKT2 chr19:40770621 C>G and SLC19A1 chr21:46934171 A>C, were identified as novel platinum response biomarkers. Finally, a pharmacogenomic database, named the Chinese Pharmacogenomic Knowledge Base (CNPKB: http://www.cnpkb.com.cn/ ), was constructed to collect all the data. In summary, a pharmacogenomic landscape and database for the Chinese population were constructed in this study, which could support personalized Chinese medicine in the future.
作者：
Like Xie;Sujun Xiao;Qinyi Zhou;Wang Chen;Zhihao Hu;...
期刊：
Journal of Traditional and Complementary Medicine,2025年 ISSN：2225-4110
通讯作者：
Yuan Li
作者机构：
[Like Xie; Sujun Xiao; Qinyi Zhou; Wang Chen; Zhihao Hu; Yizhou Liu; Xiaofeng Ma] Department of Cardiology, Nanhua Hospital affiliated to Nanhua University, Hengyang 421001, Hunan Province, China;[Yunhui Li] Zhuzhou Central Hospital, General Medicine,Zhuzhou 412001, Hunan Province, China;[Yuan Li] Nanhua Hospital Affiliated to Nanhua University, Hengyang 421001, Hunan Province, China
通讯机构：
[Yuan Li] N;Nanhua Hospital Affiliated to Nanhua University, Hengyang 421001, Hunan Province, China
关键词：
HO-1;Keap1;Nrf2;Oxidative stress;ROS
摘要：
Background Anticancer agent doxorubicin is essential for cancer treatment but often causes cardiotoxicity. Tectorigenin has shown potential cardioprotective effects, but underlying mechanisms remain unclear. We aimed to investigate whether Tectorigenin attenuates doxorubicin-induced myocardial injury.
Anticancer agent doxorubicin is essential for cancer treatment but often causes cardiotoxicity. Tectorigenin has shown potential cardioprotective effects, but underlying mechanisms remain unclear. We aimed to investigate whether Tectorigenin attenuates doxorubicin-induced myocardial injury.
Methods Doxorubicin (DOX) was utilized in C57BL/6J mice and cardiomyocytes H9c2, to establish in vivo and in vitro cardiotoxicity models, then treated with Tectorigenin. The levels of ferroptosis-related factors were measured using specific assay kits. The Liperfluo and DHE stainings were used to detect levels of lipid ROS. Cardiac function in rats was assessed using echocardiography. Immunofluorescence staining was used to detect Nrf2 nuclear translocation. ELISA assay was employed to check serum CK-MB, BNP and Tn-T levels. Cardiac injury and fibrosis were evaluated through HE and Masson stainings. Furthermore, TEM was employed to observe mitochondrial ultrastructure. Western blot and immunofluorescence staining were utilized to detect protein levels.
Doxorubicin (DOX) was utilized in C57BL/6J mice and cardiomyocytes H9c2, to establish in vivo and in vitro cardiotoxicity models, then treated with Tectorigenin. The levels of ferroptosis-related factors were measured using specific assay kits. The Liperfluo and DHE stainings were used to detect levels of lipid ROS. Cardiac function in rats was assessed using echocardiography. Immunofluorescence staining was used to detect Nrf2 nuclear translocation. ELISA assay was employed to check serum CK-MB, BNP and Tn-T levels. Cardiac injury and fibrosis were evaluated through HE and Masson stainings. Furthermore, TEM was employed to observe mitochondrial ultrastructure. Western blot and immunofluorescence staining were utilized to detect protein levels.
Results DOX induced ferroptosis in H9c2 cells concentration-dependently and time-dependently, which was alleviated by Tectorigenin treatment. ML385 or K67 abolished Tectorigenin’s inhibition on DOX-induced H9c2 cell ferroptosis. Mechanistically, Tectorigenin promoted the expressions of p62 and p-p62, leading to decreased Keap1 expression. This cascade facilitated Nrf2 nuclear translocation and subsequently elevated HO-1 and GPX4 expressions. Moreover, Tectorigenin treatment improved cardiac function, myocardial injury, fibrosis and mitochondrial function in C57BL/6J mice induced by DOX, as well as ferroptosis.
DOX induced ferroptosis in H9c2 cells concentration-dependently and time-dependently, which was alleviated by Tectorigenin treatment. ML385 or K67 abolished Tectorigenin’s inhibition on DOX-induced H9c2 cell ferroptosis. Mechanistically, Tectorigenin promoted the expressions of p62 and p-p62, leading to decreased Keap1 expression. This cascade facilitated Nrf2 nuclear translocation and subsequently elevated HO-1 and GPX4 expressions. Moreover, Tectorigenin treatment improved cardiac function, myocardial injury, fibrosis and mitochondrial function in C57BL/6J mice induced by DOX, as well as ferroptosis.
Conclusion Our findings reveal that Tectorigenin attenuates DOX-induced ferroptosis and myocardial damage by activating the p62-Keap1-Nrf2/HO-1/GPX4 axis, this may provide a therapeutic strategy for mitigating cardiotoxicity associated with chemotherapeutic agents.
Our findings reveal that Tectorigenin attenuates DOX-induced ferroptosis and myocardial damage by activating the p62-Keap1-Nrf2/HO-1/GPX4 axis, this may provide a therapeutic strategy for mitigating cardiotoxicity associated with chemotherapeutic agents.
通讯作者：
Lei Wang<&wdkj&>Ting Li<&wdkj&>Qiang He<&wdkj&>Zhijun Zhu
作者机构：
[Zhou, Guangpeng; Wei, Lin; Sun, Liying; Cao, Shuang; Zhang, Haiming] Liver Transplantation Center, Clinical Research Center for Pediatric Liver Transplantation, State Key Lab of Digestive Health, National Clinical Research Center for Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, 95 Yong'an Road, Xicheng District, Beijing, 100050, China;[Yu, Sihan] Cardiology Department, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, China;[Huang, Liangbin] Department of Breast and Thyroid Surgery, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, 410028, Hunan, China;[Seery, Samuel] Department of Humanities and Social Sciences, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China;[Seery, Samuel] School of Pharmacy, Newcastle University, Newcastle, NE1 4LP, UK
通讯机构：
[Lei Wang] B;[Ting Li; Qiang He] D;[Zhijun Zhu] L;Liver Transplantation Center, Clinical Research Center for Pediatric Liver Transplantation, State Key Lab of Digestive Health, National Clinical Research Center for Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, China<&wdkj&>Department of Hepatobiliary and Pancreaticosplenic Surgery, Medical Research Center, Beijing Organ Transplant Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China<&wdkj&>Department of Liver Transplantation, The Second Xiang-ya Hospital, Central South University, Changsha, China<&wdkj&>Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China
关键词：
Deep learning;DeepSurv;Hepatocellular carcinoma;Liver transplantation;Recurrence predictive models
摘要：
Hepatocellular carcinoma (HCC) recurrence after liver transplantation (LT) is a major contributor to mortality. We developed a recurrence prediction system for HCC patients before and after LT. Data from patients with HCC who underwent LT were retrospectively collected from three specialist centres in China. Pre- and post-operative variables were selected using support vector machine, random forest, and logistic regression (LR). Then, pre- and post-operative models were developed using three machine learning methods: LR, stacking, and two survival-based approaches. Models were evaluated using seven assessment indices, and patients were classified as either high- or low-risk based on recurrence risk. 466 patients were included and followed for a median of 51.0 months (95% CI 47.8-54.2). The pre-DeepSurv model (pre-DSM) had a C-index of 0.790 ± 0.003 during training, 0.775 ± 0.037 during testing, and 0.765 ± 0.001 and 0.819 ± 0.002 during external validation. After incorporating clinicopathologic variables, the post-DeepSurv model (post-DSM) had a 0.835 ± 0.008C-index during training, 0.812 ± 0.082 during testing, and 0.839 ± 0.001 and 0.831 ± 0.002 during external validation. The post-DSM outperformed the Milan criteria by more accurately identifying patients at high risk of recurrence. Tumour recurrence predictions also improved significantly with DeepSurv. Both pre- and post-DSMs have the potential to guide personalised surveillance strategies for LT patients with HCC.
期刊：
Journal of Nuclear Materials,2025年608:155725 ISSN：0022-3115
通讯作者：
Xian Tang<&wdkj&>Guo-Dong Cheng
作者机构：
[Hongtao Zhang; Xian Tang] School of Nuclear Science and Technology, University of South China, Hengyang 421001, China;[Qiang Li; Guo-Dong Cheng] School of Computer, University of South China, Hengyang 421001, China;[Long Yan] Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
通讯机构：
[Xian Tang; Guo-Dong Cheng] S;School of Nuclear Science and Technology, University of South China, Hengyang 421001, China<&wdkj&>School of Computer, University of South China, Hengyang 421001, China
摘要：
The effect of temperatures ranging from 0 K to 1000 K on the positron lifetime of He- or H-vacancy complexes in 3 C -SiC are studied using first-principles calculations. We observed a steady decrease in the formation energy of H-vacancy complexes upon additional H introduction, in contrast to the variation tendency of formation energies seen in He-vacancy complexes. The intrinsic vacancies ( V Si , V C , and V Si + C ) exhibit different decrease in positron lifetime with the addition of He or H atoms, with the effect of He being more pronounced. Moreover, V C and its impurity-vacancy complexes are almost incapable of trapping delocalized positrons, with positron lifetimes close to those of the bulk. Positron lifetime calculations for the complexes show no significant temperature dependence from 0 K to 1000 K, except in the case of V C . The above results are interpreted by analysis of electron density, positron density, positron ground-state energy, and positron trapping energy analysis. The results provide a reference for future in-situ temperature-dependent positron lifetime experiments on impurity-vacancy complexes in 3 C -SiC.
The effect of temperatures ranging from 0 K to 1000 K on the positron lifetime of He- or H-vacancy complexes in 3 C -SiC are studied using first-principles calculations. We observed a steady decrease in the formation energy of H-vacancy complexes upon additional H introduction, in contrast to the variation tendency of formation energies seen in He-vacancy complexes. The intrinsic vacancies ( V Si , V C , and V Si + C ) exhibit different decrease in positron lifetime with the addition of He or H atoms, with the effect of He being more pronounced. Moreover, V C and its impurity-vacancy complexes are almost incapable of trapping delocalized positrons, with positron lifetimes close to those of the bulk. Positron lifetime calculations for the complexes show no significant temperature dependence from 0 K to 1000 K, except in the case of V C . The above results are interpreted by analysis of electron density, positron density, positron ground-state energy, and positron trapping energy analysis. The results provide a reference for future in-situ temperature-dependent positron lifetime experiments on impurity-vacancy complexes in 3 C -SiC.
作者机构：
[Xiao, Zhenghui; Li, Xiao; Xiao, ZH] Univ South China, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Yan, Haipeng; Wang, Xiangyu; Li, Xun; Xiao, Zhenghui; Luo, Ting; Li, Xiao; Yu, Kun; Lu, Xiulan; Xiao, ZH; Xie, Longlong] Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Dept Pediat Intens Care Unit PICU,Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Yan, Haipeng; Wang, Xiangyu; Li, Xun; Xiao, Zhenghui; Luo, Ting; Li, Xiao; Yu, Kun; Lu, Xiulan; Xiao, ZH; Xie, Longlong] Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Hunan Prov Key Lab Emergency Med Children,Xiangya, Changsha, Hunan, Peoples R China.
通讯机构：
[Li, X] C;[Xiao, ZH ] U;Univ South China, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Dept Pediat Intens Care Unit PICU,Xiangya Sch Med, Changsha, Hunan, Peoples R China.;Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Hunan Prov Key Lab Emergency Med Children,Xiangya, Changsha, Hunan, Peoples R China.
关键词：
children;health-related quality of life;hemophagocytic lymphohistiocytosis;PedsQL
摘要：
PURPOSE: Evaluating the post-discharge health-related quality of life (HRQoL) in hemophagocytic lymphohistiocytosis (HLH) and exploring its influencing factors. PATIENTS AND METHODS: The study was conducted at a regional pediatric medical center and involved pediatric patients diagnosed with HLH between July 2017 and July 2022. Healthy children of the same age and sex were included as the control group. The HRQoL and its associated factors in pediatric patients were assessed using the PedsQL 4.0 Parent Proxy Report and a general information survey. RESULTS: In the first year following diagnosis, psychological health and overall score in HLH patients were worse than those of the control group. However, scores for emotional functioning, school functioning, physical health, psychosocial health, and overall scores in the HLH group increased over the years since diagnosis. By the 5-year post diagnosis, there were no significant differences between the HLH group and the control group in social functioning, school functioning, physical health, psychosocial health, and overall scores (p>0.05). Generalized linear model analysis revealed that HLH patients who underwent transplantation have worse social functioning, physical health, overall score, while HLH patients with HLH recurrence have worse social functioning, psychosocial health, overall score (p<0.05). CONCLUSION: The HRQoL of HLH patients is compromised after discharge; however, it progressively returns to levels comparable to those of healthy cohorts over time since diagnosis. Transplantation, and HLH recurrence are identified as factors affecting the HRQoL in HLH patients.
作者机构：
[Tan, Wenfa; Ding, DX; Ding, Dexin; Yu, Huang; Hu, Nan] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.;[Yan, Qingyun; Zhang, Dandan; Yan, QY; He, Zhili; Yu, Huang; Liu, Huanping; Chen, Pubo] Sun Yat Sen Univ, Marine Synthet Ecol Res Ctr, China ASEAN Belt & Rd Joint Lab Mariculture Techn, Southern Marine Sci & Engn Guangdong Lab Zhuhai,G, Zhuhai 519082, Peoples R China.;[Liu, Shengwei] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England.;[Hu, Ruiwen] Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA.;[Zhou, Qiang] Jishou Univ, Coll Biol & Environm Sci, Jishou 416000, Xiangxi Tujia &, Peoples R China.
通讯机构：
[Yan, QY ] S;[Ding, DX ] U;Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Mi, Hengyang 421001, Peoples R China.;Sun Yat Sen Univ, Marine Synthet Ecol Res Ctr, China ASEAN Belt & Rd Joint Lab Mariculture Techn, Southern Marine Sci & Engn Guangdong Lab Zhuhai,G, Zhuhai 519082, Peoples R China.
关键词：
arsenic methylation;dissimilatory nitrate reduction to ammonium;microorganism enrichment;nitrogen fixation;phytoremediation
摘要：
Plants can recruit microorganisms to enhance soil arsenic (As) removal and nitrogen (N) turnover, but how microbial As methylation in the rhizosphere is affected by N biotransformation is not well understood. Here, we used acetylene reduction assay, arsM gene amplicon, and metagenome sequencing to evaluate the influence of N biotransformation on As methylation in the rhizosphere of Vetiveria zizanioides, a potential As hyperaccumulator. V. zizanioides was grown in mining soils (MS) and artificial As-contaminated soils (AS) over two generations in a controlled pot experiment. Results showed that the content of dimethylarsinic acid in the rhizosphere was significantly positively correlated with the rate of N fixation and the activity of nitrite reductase. The As-methylating species (e.g., Flavisolibacter and Paraflavitalea) were significantly enriched in the root-associated compartments in the second generation of MS and AS. Notably, higher abundance of genes involved in N fixation (nifD, nifK) and dissimilatory nitrate reduction to ammonium (narG/H, nirB/D/K/S) was detected in the second generation of MS than in the first generation. The metabolic pathway analysis further demonstrated that N fixing-stimulative and DNRA-stimulative As-methylating species could provide ammonium to enhance the synthesis of S-adenosyl-l-methionine, serving as methyl donors for soil As methylation. This study highlights two important N conversion-stimulative As-methylating pathways and has important implications for enhancing phytoremediation in As-contaminated soils.
期刊：
Annals of Medicine,2025年57(1):2474730 ISSN：0785-3890
通讯作者：
Jian Wu<&wdkj&>Shaohang Cai
作者机构：
[Liao, Xiaoyang] Second Department of Elderly Respiratory, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, Southern Medical University, Guangzhou, China;[Chen, Suling; Xu, Yuyuan; Zhong, Huiqun; Xu, Xuwen; Peng, Jie; Cai, Shaohang] Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China;[Xie, Jingfang] Department of Geriatrics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, Southern Medical University, Guangzhou, China;[Ou, Lang] Department of hepatobiliary surgery, Affiliated Nanhua Hospital, University of South China, Hengyang, China;[Zuo, Xiang] Division of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China
通讯机构：
[Jian Wu] S;[Shaohang Cai] D;Second Department of Elderly Respiratory, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, Southern Medical University, Guangzhou, China<&wdkj&>School of Medicine, South China University of Technology, Guangzhou, China<&wdkj&>Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China
摘要：
BACKGROUND: Previous studies have shown changes in gut microbiota after human immunodeficiency virus (HIV) infection, but there is limited research linking the gut microbiota of people living with HIV (PLWHIV) to metabolic diseases. METHODS: A total of 103 PLWHIV were followed for 48 weeks of anti-retroviral therapy (ART), with demographic and clinical data collected. Gut microbiome analysis was conducted using metagenomic sequencing of fecal samples from 12 individuals. Nonalcoholic fatty liver disease (NAFLD) was diagnosed based on controlled attenuation parameter (CAP) values of 238 dB/m from liver fibro-scans. Participants were divided based on the presence of metabolic disorders, including NAFLD, overweight, and hyperlipidemia. Akkermansia abundance in stool samples was measured using RT-qPCR, and Pearson correlation and logistic regression were applied for analysis. RESULTS: Metagenomic sequencing revealed a significant decline in gut Akkermansia abundance in PLWHIV with NAFLD. STAMP analysis of public datasets confirmed this decline after HIV infection, while KEGG pathway analysis identified enrichment of metabolism-related genes. A prospective cohort study with 103 PLWHIV followed for 48 weeks validated these findings. Akkermansia abundance was significantly lower in participants with NAFLD, overweight, and hyperlipidemia at baseline, and it emerged as an independent predictor of NAFLD and overweight. Negative correlations were observed between Akkermansia abundance and both CAP values and body mass index (BMI) at baseline and at week 48. At the 48-week follow-up, Akkermansia remained a predictive marker for NAFLD. CONCLUSIONS: Akkermansia abundance was reduced in PLWHIV with metabolic disorders and served as a predictive biomarker for NAFLD progression over 48 weeks of ART.
关键词：
Acute CO poisoning;Carbon monoxide;Gaseous signaling molecule;Hyperbaric oxygen therapy
摘要：
Carbon monoxide (CO) is a colorless, odorless, toxic, and polluting gas produced by the incomplete combustion of carbon-containing substances such as coal and petroleum. CO is also a gaseous signaling molecule, which participates in and regulates a series of physiological and pathological processes. In this work, two kinds of CO-activated fluorescence probes 2 and 4 were designed and synthesized for the rapid detection of CO and evaluation of acute CO poisoning by hyperbaric oxygen therapy. Specifically, 2 and 4 both used the allyl formate group as the CO response unit and when they reacted with CO, the response group broke off and the fluorescence burst effect subsided, allowing them to regain fluorescence. In addition, because of the excellent optical properties of 4 (especially, colorimetric and near-infrared (NIR) fluorescence), subsequent experiments were performed with 4 as a representative for imaging CO in zebrafish and mice with impressive results. Interestingly, 4 has been successfully used for assessing acute CO poisoning by hyperbaric oxygen therapy with satisfactory results, revealing practical application in the connection between CO and hyperbaric oxygen therapy.
Carbon monoxide (CO) is a colorless, odorless, toxic, and polluting gas produced by the incomplete combustion of carbon-containing substances such as coal and petroleum. CO is also a gaseous signaling molecule, which participates in and regulates a series of physiological and pathological processes. In this work, two kinds of CO-activated fluorescence probes 2 and 4 were designed and synthesized for the rapid detection of CO and evaluation of acute CO poisoning by hyperbaric oxygen therapy. Specifically, 2 and 4 both used the allyl formate group as the CO response unit and when they reacted with CO, the response group broke off and the fluorescence burst effect subsided, allowing them to regain fluorescence. In addition, because of the excellent optical properties of 4 (especially, colorimetric and near-infrared (NIR) fluorescence), subsequent experiments were performed with 4 as a representative for imaging CO in zebrafish and mice with impressive results. Interestingly, 4 has been successfully used for assessing acute CO poisoning by hyperbaric oxygen therapy with satisfactory results, revealing practical application in the connection between CO and hyperbaric oxygen therapy.
作者：
Guo, Xi;Contreras, Miguel Angel Martin;Chen, Xun;Xiang, Dong
期刊：
中国物理C,2025年49(1):013104 ISSN：1674-1137
通讯作者：
Guo, X
作者机构：
[Guo, Xi; Chen, Xun; Xiang, Dong; Contreras, Miguel Angel Martin; Guo, X] Univ South China, Sch Nucl Sci & Technol, Hengyang 421000, Peoples R China.
通讯机构：
[Guo, X ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421000, Peoples R China.
关键词：
baryon spectroscopy;AdS/QCD;holographic QCD
摘要：
In this study, we discuss the description of neutral Sigma baryons with I(J(P))= 1(1/2(+))and I(J(P)) = 1(3/2(+)) using two bottom-up approaches: the deformed background and static dilaton models. In both models, we consider a non-linear Regge trajectory extension motivated by the strange nature of Sigma baryons. We find that both models describe these systems with an RMS error smaller than 10%. We also perform a configurational entropy calculation in both models to discuss hadronic stability.
作者机构：
[Zhang, Le; Cai, Fei-Yang] Hubei Normal Univ, Coll Phys & Elect Sci, Huangshi 435002, Peoples R China.;[Chen, Xun; Chen, X] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Chen, X ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
关键词：
holographic QCD;hybrid potential;heavy quark
摘要：
Using gauge/gravity duality, we studied the exotic hybrid pseudopotentials at finite temperature and chemical potential. The Sigma hybrid meson can be described by a model including an object called "defect'' on a string linking the quark and antiquark. The Sigma(-)(u) hybrid potential at zero temperature and chemical potential was first proposed by Andreev and is perfectly described. In this study, we extended the aforementioned model to finite chemical potential and compared the separate distance and pseudopotentials of Sigma(+)(g) and Sigma u-. Unlike the Sigma(+)(g) ground state, the Sigma(-)(u) hybrid pseudopotentials no longer exhibit Coulomb-like behavior at short distances. In addition, temperature and chemical potential have a significant impact on the Sigma(-)(u) hybrid pseudopotentials. The screen distances and hybrid pseudopotentials of Sigma(-)(u) significantly decrease when increasing temperature and chemical potential. We represented the melting diagram of Sigma(+)(g) and Sigma(-)(u) in the T-mu plane and confirmed that the quark-antiquark pair in Sigma(-)(u) excited state is easier to melt than that in Sigma(+)(g) ground state.
作者机构：
[Ochoa-Ricoux, Juan Pedro; Abusleme, Angel; Campeny, Agustin; Kuleshov, Sergey; Herrera, Rafael; Walker, Pablo] Millennium Inst SubAtom Phys, High Energy Frontier SAPHIR, ANID, Santiago, Chile.;[Kazarian, Narine; Mayilyan, Davit; Ioannisian, Ara] Yerevan Phys Inst, Yerevan, Armenia.;[Molla, Marta Colomer; Gao, Feng; Yang, Yifan; Clerbaux, Barbara; Datta, Jaydeep; Petitjean, Pierre-Alexandre] Univ Libre Bruxelles, Brussels, Belgium.;[Chimenti, Pietro] Univ Estadual Londrina, Londrina, Parana, Brazil.;[Nunokawa, Hiroshi] Pontificia Univ Catolica Rio Janeiro, Rio De Janeiro, Brazil.
通讯机构：
[Abusleme, A ] M;Millennium Inst SubAtom Phys, High Energy Frontier SAPHIR, ANID, Santiago, Chile.;Pontificia Univ Catolica Chile, Santiago, Chile.
关键词：
energy resolution;liquid scintillator detector;JUNO;simulation;reconstruction
摘要：
This paper presents an energy resolution study of the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of the liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The results of this study reveal an energy resolution of 2.95% at 1 MeV. Furthermore, this study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data collection. Moreover, it provides a guideline for comprehending the energy resolution characteristics of liquid scintillator-based detectors.
作者机构：
[Zhe Yu; Teng Li; Xiya Yang; Xindi Zhang; Haoying Jiang; Lin Zhu; Bo Yang; Yang Wang; Jiekun Luo; Xueping Yang] Institute of Integrative Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China;Department of Neurology, Xiangya Hospital, Central South University, Jiangxi (National Regional Center for Neurological Diseases), Nanchang, 330038, China;Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330038, China;The First Affiliated Hospital, Children's Medical Centre, Hengyang Medical School, University of South China, Hengyang, 421001, China;Institute of Integrative Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Jiangxi (National Regional Center for Neurological Diseases), Nanchang, 330038, China
通讯机构：
[En Hu] I;Institute of Integrative Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Jiangxi (National Regional Center for Neurological Diseases), Nanchang, 330038, China<&wdkj&>NATCM Key Laboratory of TCM Gan, Xiangya Hospital, Central South University, Changsha, 410008, China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, China
关键词：
Traditional Chinese medicine;Prescription formulation;Gradient weighting strategy;Multiple targets;Intracerebral hemorrhage
摘要：
Traditional Chinese medicine (TCM) exerts integrative effects on complex diseases owing the characteristics of multiple components with multiple targets. However, the syndrome-based system of diagnosis and treatment in TCM can easily lead to bias because of varying medication preferences among physicians, which has been a major challenge in the global acceptance and application of TCM. Therefore, a standardized TCM prescription system needs to be explored to promote its clinical application. In this study, we first developed a gradient weighted disease-target-herbal ingredient-herb network to aid TCM formulation. We tested its efficacy against intracerebral hemorrhage (ICH). First, the top 100 ICH targets in the GeneCards database were screened according to their relevance scores. Then, SymMap and Traditional Chinese Medicine Systems Pharmacology (TCMSP) databases were applied to find out the target-related ingredients and ingredient-containing herbs, respectively. The relevance of the resulting ingredients and herbs to ICH was determined by adding the relevance scores of the corresponding targets. The top five ICH therapeutic herbs were combined to form a tailored TCM prescriptions. The absorbed components in the serum were detected. In a mouse model of ICH, the new prescription exerted multifaceted effects, including improved neurological function, as well as attenuated neuronal damage, cell apoptosis, vascular leakage, and neuroinflammation. These effects matched well with the core pathological changes in ICH. The multi-targets-directed gradient-weighting strategy presents a promising avenue for tailoring precise, multipronged, unbiased, and standardized TCM prescriptions for complex diseases. This study provides a paradigm for advanced achievements-driven modern innovation in TCM concepts.
Traditional Chinese medicine (TCM) exerts integrative effects on complex diseases owing the characteristics of multiple components with multiple targets. However, the syndrome-based system of diagnosis and treatment in TCM can easily lead to bias because of varying medication preferences among physicians, which has been a major challenge in the global acceptance and application of TCM. Therefore, a standardized TCM prescription system needs to be explored to promote its clinical application. In this study, we first developed a gradient weighted disease-target-herbal ingredient-herb network to aid TCM formulation. We tested its efficacy against intracerebral hemorrhage (ICH). First, the top 100 ICH targets in the GeneCards database were screened according to their relevance scores. Then, SymMap and Traditional Chinese Medicine Systems Pharmacology (TCMSP) databases were applied to find out the target-related ingredients and ingredient-containing herbs, respectively. The relevance of the resulting ingredients and herbs to ICH was determined by adding the relevance scores of the corresponding targets. The top five ICH therapeutic herbs were combined to form a tailored TCM prescriptions. The absorbed components in the serum were detected. In a mouse model of ICH, the new prescription exerted multifaceted effects, including improved neurological function, as well as attenuated neuronal damage, cell apoptosis, vascular leakage, and neuroinflammation. These effects matched well with the core pathological changes in ICH. The multi-targets-directed gradient-weighting strategy presents a promising avenue for tailoring precise, multipronged, unbiased, and standardized TCM prescriptions for complex diseases. This study provides a paradigm for advanced achievements-driven modern innovation in TCM concepts.
摘要：
Peptides exhibit various biological activities, including biorecognition, cell targeting, and tumor penetration, and can stimulate immune cells to elicit immune responses for tumor immunotherapy. Peptide self-assemblies and peptide-functionalized nanocarriers can reduce the effect of various biological barriers and the degradation by peptidases, enhancing the efficiency of peptide delivery and improving antitumor immune responses. To date, the design and development of peptides with various functionalities have been extensively reviewed for enhanced chemotherapy; however, peptide-mediated tumor immunotherapy using peptides acting on different immune cells, to the knowledge, has not yet been summarized. Thus, this work provides a review of this emerging subject of research, focusing on immunomodulatory anticancer peptides. This review introduces the role of peptides in the immunomodulation of innate and adaptive immune cells, followed by a link between peptides in the innate and adaptive immune systems. The peptides are discussed in detail, following a classification according to their effects on different innate and adaptive immune cells, as well as immune checkpoints. Subsequently, two delivery strategies for peptides as drugs are presented: peptide self-assemblies and peptide-functionalized nanocarriers. The concluding remarks regarding the challenges and potential solutions of peptides for tumor immunotherapy are presented. This work introduces the role of peptides in immune regulation of innate and adaptive immune cells, as well as immune checkpoints. Then this work introduces two strategies for delivering polypeptides: peptide self-assemblies, and peptide-functionalized nanocarriers. Finally, the challenges and prospects of peptides in tumor immunotherapy are summarized. image
