期刊：
CHEMICAL SOCIETY REVIEWS,2012年41(2):797-828 ISSN：0306-0012
通讯作者：
Wang, GP
作者机构：
[Wang, Guoping] Univ S China, Coll Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Guoping; Zhang, Lei; Zhang, Jiujun] Natl Res Council Canada, Inst Fuel Cell Innovat, Vancouver, BC V6T 1W5, Canada.
通讯机构：
[Wang, GP] Univ S China, Coll Chem Engn, Hengyang 421001, Peoples R China.
摘要：
In this critical review, metal oxides-based materials for electrochemical supercapacitor (ES) electrodes are reviewed in detail together with a brief review of carbon materials and conducting polymers. Their advantages, disadvantages, and performance in ES electrodes are discussed through extensive analysis of the literature, and new trends in material development are also reviewed. Two important future research directions are indicated and summarized, based on results published in the literature: the development of composite and nanostructured ES materials to overcome the major challenge posed by the low energy density of ES (476 references).
摘要：
The adsorption of chromium (VI) ions from aqueous solution by ethylenediamine-modified cross-linked magnetic chitosan resin (EMCMCR) was studied in a batch adsorption system. Chromium (VI) removal is pH dependent and the optimum adsorption was observed at pH 2.0. The adsorption rate was extremely fast and the equilibrium was established within 6-10 min. The adsorption data could be well interpreted by the Langmuir and Temkin model. The maximum adsorption capacities obtained from the Langmuir model are 51.813 mg g(-1), 48.780 mg g(-1) and 45.872 mg g(-1) at 293, 303 and 313 K, respectively. The adsorption process could be described by pseudo-second-order kinetic model. The intraparticle diffusion study revealed that film diffusion might be involved in the present case. Thermodynamic parameters revealed the feasibility, spontaneity and exothermic nature of adsorption. The sorbents were successfully regenerated using 0.1 N NaOH solutions. (C) 2010 Elsevier B.V. All rights reserved.
摘要：
Leaf carbon isotope ratios and leaf mineral composition (Ca, K, Mg, Mn, N, and P) were measured on the dominant species along an irradiance cline in a subtropical monsoon forest of southern China. This irradiance cline resulted from disturbance caused by fuel-harvesting. Leaf carbon isotope ratios increased from undisturbed to disturbed sites for all species, indicating that leaf intercellular CO2 concentrations decreased and leaf water use efficiencies increased along this cline. Nitrogen and magnesium levels were lower in leaves of species on the disturbed sites, but there were no clear patterns for calcium, potassium, phosphorus or manganese.
作者机构：
[Thorndike, E. H.; Ma, T. ; Ambrose, D.J. ] Univ Rochester, Rochester, NY 14627 USA.;[Peters, K.; Goetzen, K. ] GSI Helmholtzctr Heavy Ion Res GmbH, D-64291 Darmstadt, Germany.;[ Bai, J.Z. ; Li, W.D. ; Dai, H.L. ; Qin, X. S.; Zhu, K.; Liu, C.X. ; Han, Y.L. ; Ablikim, M. ; Chen, J.C. ; Zhao, J. W.; Qiu, J. F.; Ping, R. G.; Cheng, H.P. ; Zhang, J. Z.; Li, G.; Qin, Z. H.; Yang, H. X.; Tang, X.; Jiang, X.S. ; Wang, L. S.; Zhang, J. Y.; Li, F. ; Zhang, S. H.; Liu, Zhiqing; Li, J.C. ; Zhuang, J.; Li, H.B. ; Ji, X.L. ; Luo, X.L. ; Chu, Y. P.; Wu, Z.; Wang, B.; Zhao, G.; Wang, P. L.; Dai, J.P. ; Gong, W.X. ; Hu, H.M. ; Ma, X.Y. ; Dong, L.Y. ; Lu, Y.P. ; Gu, M.H. ; Jin, S. ; Zhang, B. Y.; Li, X.N. ; Zhu, Y. S.; Zheng, J. P.; Shen, X. Y.; Huang, L. ; Zhao, Y. B.; Fang, J. ; Wu, L. H.; Zhou, L.; Mo, X.H. ; Cao, G.F. ; Li, W.G. ; Lai, W. ; Zhang, X. J.; Zhang, B. X.; Jiang, L.L. ; Wang, Z. G.; Zou, J. H.; Lou, X.C. ; Zhang, Y.; Fu, C.D. ; Sun, S. S.; Ye, H.; An, F.F. ; Wang, Q. J.; Zhang, Y. H.; Kuehn, W. ; Lv, M. ; Cai, X. ; Liu, Zhiqiang ; Zhao, T. C.; Wang, Z. Y.; Chang, J.F. ; Fang, S.S. ; Dong, M.Y. ; Ma, Q.M. ; Deng, Z.Y. ; Wang, P.; Qian, S.; Zhang, J. Q.; Calcaterra, A. ; Ji, X.B. ; Zou, B. S.; Achasov, M.N. ; An, Q. ; Xie, Y. G.; Ye, M.; Liu, Q. ; Jin, D.P. ; Wang, L. L.; Ma, H.L. ; Ji, Q. ; Liu, H.M. ; Zhu, K. J.; Wang, Y. F.; Min, J. ; Liu, B.J. ; Sun, Y. Z.; Li, Lei ; Rong, G.; Hou, Z.L. ; Zhang, J. W.; Li, Q.J. ; Heng, Y.K. ; Wang, K.; Xu, G. F.; Zhu, Z. A.; Yang, Y. Z.; Kavatsyuk, M. ; He, M. ; Zhao, Ling; Zhang, H. Y.; Bian, J.M. ; Li, C.H. ; Zhang, D. H.; Sun, G. X.; Wen, S. P.; Zhang, C. C.; Ning, Z.; Xue, Z.; Sun, Z. J.; Liu, Fang ; Wang, Z.; Hussain, T. ; Leyhe, M. ; Chen, Y.B. ; Li, G. ; Lu, J.G. ; Zhao, Q.; Mao, Z.P. ; Yu, B. X.] Inst High Energy Phys, Beijing 100049, Peoples R China.;[Zafar, A. A.; Malik, Q.A. ; Hu, T. ; Rashid, K. H.] Univ Punjab, Lahore 54590, Pakistan.;[Xia, L. G.; Zhu, X. L.; Liao, G.R. ; Jing, F.F. ; Li, K. ; Wang, X. F.; Gao, Y. ] Tsinghua Univ, Beijing 100084, Peoples R China.
通讯机构：
[Ablikim, M] Inst High Energy Phys, Beijing 100049, Peoples R China.
关键词：
Beijing Electron Positron Collider - Center-of-mass energies - Data sample - Final state - Invariant-mass spectra - Mass spectra - Production cross section - Upper limits
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience,2012年32(9):3044-3057 ISSN：0270-6474
通讯作者：
Liu, WL
作者机构：
[Liu, Wenlan; Liu, Ke J.; Jin, Xinchun; Liu, Jie] Univ New Mexico, Coll Pharm, Hlth Sci Ctr, Albuquerque, NM 87131 USA.;[Liu, Ke J.] Univ New Mexico, Dept Neurol, Hlth Sci Ctr, Albuquerque, NM 87131 USA.;[Liu, Jie] Univ South China, Sch Med, Dept Med Microbiol & Immunol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liu, WL] Univ New Mexico, Coll Pharm, Hlth Sci Ctr, Albuquerque, NM 87131 USA.
摘要：
Blood-brain barrier (BBB) disruption occurs early enough to be within the thrombolytic time window, and this early ischemic BBB damage is closely associated with hemorrhagic transformation and thus emerging as a promising target for reducing the hemorrhagic complications of thrombolytic stroke therapy. However, the mechanisms underlying early ischemic BBB damage remain poorly understood. Here, we investigated the early molecular events of ischemic BBB damage using in vitro oxygen-glucose deprivation (OGD) and in vivo rat middle cerebral artery occlusion (MCAO) models. Exposure of bEND3 monolayer to OGD for 2 h significantly increased its permeability to FITC-labeled dextran and promoted the secretion of metalloproteinase-2 and -9 (MMP-2/9) and cytosolic translocation of caveolin-1 (Cav-1). This same OGD treatment also led to rapid degradation of tight junction protein occludin and dissociation of claudin-5 from the cytoskeleton, which contributed to OGD-induced endothelial barrier disruption. Using selective MMP-2/9 inhibitor SB-3CT (2-[[(4-phenoxyphenyl)sulfonyl]methyl]-thiirane) or their neutralizing antibodies or Cav-1 siRNA, we found that MMP-2 was the major enzyme mediating OGD-induced occludin degradation, while Cav-1 was responsible for claudin-5 redistribution. The interaction between Cav-1 and claudin-5 was further confirmed by coimmunoprecipitation. Consistent with these in vitro findings, we observed fluorescence tracer extravasation, increased gelatinolytic activity, and elevated interstitial MMP-2 levels in ischemic subcortical tissue after 2 h MCAO. Moreover, occludin protein loss and claudin-5 redistribution were detected in ischemic cerebromicrovessels. These data indicate that cerebral ischemia initiates two rapid parallel processes, MMP-2-mediated occludin degradation and Cav-1-mediated claudin-5 redistribution, to cause BBB disruption at early stroke stages relevant to acute thrombolysis.
摘要：
Atherosclerosis is a chronic disease characterized by the deposition of excessive cholesterol in the arterial intima. Macrophage foam cells play a critical role in the occurrence and development of atherosclerosis. The generation of these cells is associated with imbalance of cholesterol influx, esterification and efflux. CD36 and scavenger receptor class A (SR-A) are mainly responsible for uptake of lipoprotein-derived cholesterol by macrophages. Acyl coenzyme A:cholesterol acyltransferase-1 (ACAT1) and neutral cholesteryl ester hydrolase (nCEH) regulate cholesterol esterification. ATP-binding cassette transporters A1 (ABCA1), ABCG1 and scavenger receptor BI (SR-BI) play crucial roles in macrophage cholesterol export When inflow and esterification of cholesterol increase and/or its outflow decrease, the macrophages are ultimately transformed into lipid-laden foam cells, the prototypical cells in the atherosclerotic plague. The aim of this review is to describe what is known about the mechanisms of cholesterol uptake, esterification and release in macrophages. An increased understanding of the process of macrophage foam cell formation will help to develop novel therapeutic interventions for atherosclerosis. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
作者：
Nakajima, Hideaki;Uchida, Kenzo;Guerrero, Alexander Rodriguez;Watanabe, Shuji;Sugita, Daisuke;Takeura, Naoto;Yoshida, Ai;Long, Guang;Wright, Karina T.;Johnson, William E. B.;Baba, Hisatoshi
期刊：
JOURNAL OF NEUROTRAUMA,2012年29(8):1614-1625 ISSN：0897-7151
通讯作者：
Nakajima, H
作者机构：
[Takeura, Naoto; Uchida, Kenzo; Sugita, Daisuke; Guerrero, Alexander Rodriguez; Baba, Hisatoshi; Yoshida, Ai; Watanabe, Shuji; Nakajima, Hideaki] Univ Fukui, Dept Orthopaed & Rehabil Med, Fac Med Sci, Fukui 9101193, Japan.;[Wright, Karina T.] Keele Univ, Inst Sci & Technol Med, RJAH Orthopaed Hosp, Oswestry, Shrops, England.;[Long, Guang] Univ S China, Physiol Lab, Hengyang, Peoples R China.;[Johnson, William E. B.] Aston Univ, Birmingham B4 7ET, W Midlands, England.
通讯机构：
[Nakajima, H] Univ Fukui, Dept Orthopaed & Rehabil Med, Fac Med Sci, Matsuoka Shimoaizuki 23-3, Fukui 9101193, Japan.
关键词：
bone marrow;macrophage;mesenchymal stem cell;spinal cord injury;transplantation
摘要：
Mesenchymal stem cells (MSC) derived from bone marrow can potentially reduce the acute inflammatory response in spinal cord injury (SCI) and thus promote functional recovery. However, the precise mechanisms through which transplanted MSC attenuate inflammation after SCI are still unclear. The present study was designed to investigate the effects of MSC transplantation with a special focus on their effect on macrophage activation after SCI. Rats were subjected to T9-T10 SCI by contusion, then treated 3 days later with transplantation of 1.0 x 10(6) PKH26-labeled MSC into the contusion epicenter. The transplanted MSC migrated within the injured spinal cord without differentiating into glial or neuronal elements. MSC transplantation was associated with marked changes in the SCI environment, with significant increases in IL-4 and IL-13 levels, and reductions in TNF-alpha and IL-6 levels. This was associated simultaneously with increased numbers of alternatively activated macrophages (M2 phenotype: arginase-1- or CD206-positive), and decreased numbers of classically activated macrophages (M1 phenotype: iNOS- or CD16/32-positive). These changes were associated with functional locomotion recovery in the MSC-transplanted group, which correlated with preserved axons, less scar tissue formation, and increased myelin sparing. Our results suggested that acute transplantation of MSC after SCI modified the inflammatory environment by shifting the macrophage phenotype from M1 to M2, and that this may reduce the effects of the inhibitory scar tissue in the subacute/chronic phase after injury to provide a permissive environment for axonal extension and functional recovery.
作者：
Ablikim, M.;Achasov, M. N.;Albayrak, O.;Ambrose, D. J.;An, F. F.;An, Q.;Bai, J. Z.;Ferroli, R. Baldini;Ban, Y.;Becker, J.;Bennett, J. V.;Bertani, M.;Bian, J. M.;Boger, E.;Bondarenko, O.;Boyko, I.;Braun, S.;Briere, R. A.;Bytev, V.;Cai, H.
作者机构：
[Thorndike, E. H.; Muramatsu, H.; Ambrose, D. J.] Univ Rochester, Rochester, NY 14627 USA.;[Peters, K.; Goetzen, K.] GSI Helmholtzctr Heavy Ion Res GmbH, D-64291 Darmstadt, Germany.;[Bai, J. Z.; Li, W. D.; Dai, H. L.; Qin, X. S.; Zhu, K.; Liu, C. X.; Han, Y. L.; Ablikim, M.; Chen, J. C.; Zhao, J. W.; Qiu, J. F.; Ping, R. G.; Chen, G.; He, K. L.; Zhang, J. Z.; Li, G.; Qin, Z. H.; Yang, H. X.; Song, W. M.; Tang, X.; Jin, S.; Wang, L. S.; Zhang, J. Y.; Li, F.; Zhang, S. H.; Liu, Zhiqing; Li, J. C.; Zhuang, J.; Li, H. B.; Ji, X. L.; Luo, X. L.; Chu, Y. P.; Wu, Z.; Yuan, Y.; Wang, B.; Zhao, G.; Wang, P. L.; Dai, J. P.; Gong, W. X.; Li, K.; Hu, H. M.; Ma, X. Y.; Dong, L. Y.; Lu, Y. P.; Gu, M. H.; Jiang, X. S.; Zhang, B. Y.; Li, X. N.; Zhu, Y. S.; Zheng, J. P.; Shen, X. Y.; Huang, L.; Zhao, Y. B.; Fang, J.; Wu, L. H.; Zhou, L.; Mo, X. H.; Cao, G. F.; Li, W. G.; Lai, W.; Zhang, X. J.; Zhang, B. X.; Jiang, L. L.; Zhang, J. L.; Wang, Z. G.; Zou, J. H.; Lou, X. C.; Zhang, Y.; Fu, C. D.; Sun, S. S.; Ye, H.; An, F. F.; Wang, Q. J.; Zhang, Y. H.; Wu, N.; Lv, M.; Cai, X.; Liu, Z. A.; Zhao, T. C.; Wang, Z. Y.; Chang, J. F.; Fang, S. S.; Dong, M. Y.; Ma, Q. M.; Deng, Z. Y.; Wang, P.; Qian, S.; Zhang, J. Q.; Ji, X. B.; Chen, H. S.; Zou, B. S.; Ma, S.; Song, X. Y.; Ouyang, Q.; Sheng, H. Y.; Xie, Y. G.; Ye, M.; Xiu, Q. L.; Jin, D. P.; Wang, L. L.; Ma, H. L.; Li, Q. J.; Liu, H. M.; Zhu, K. J.; Wang, Y. F.; Min, T. J.; Liu, B. J.; Sun, Y. Z.; Li, Lei; Rong, G.; Hou, Z. L.; Zhang, J. W.; Ji, Q.; Heng, Y. K.; Wang, K.; Xu, G. F.; Zhu, Z. A.; Ma, T.; Chen, M. L.; Zhao, Ling; Zhang, H. Y.; Min, J.; Yuan, C. Z.; Li, C. H.; Zhang, D. H.; Sun, G. X.; Wen, S. P.; Zhang, C. C.; Ning, Z.; Xue, Z.; Sun, Z. J.; Liu, Fang; Wang, Z.; Hu, T.; He, M.; Chen, Y. B.; Liu, Zhiqiang; Lu, J. G.; Zhao, Q.; Mao, Z. P.; Yu, B. X.] Inst High Energy Phys, Beijing 100049, Peoples R China.;[Zafar, A. A.; Malik, Q. A.; Hussain, T.; Rashid, K. H.] Univ Punjab, Lahore 54590, Pakistan.;[Xia, L. G.; Zhu, X. L.; Liao, G. R.; Jing, F. F.; Liu, K.; Wang, X. F.; Gao, Y.] Tsinghua Univ, Beijing 100084, Peoples R China.
通讯机构：
[Ablikim, M] Inst High Energy Phys, Beijing 100049, Peoples R China.
摘要：
We report on a study of the process e(+)e(-) -> pi(+/-) (D (D) over bar*)(-/+) at root s = 4.26 GeV using a 525 pb(-1) data sample collected with the BESIII detector at the BEPCII storage ring. A distinct charged structure is observed in the (D (D) over bar*)(-/+) invariant mass distribution. When fitted to a mass- dependent- width Breit- Wigner line shape, the pole mass and width are determined to be M-pole (3883: 9 +/- 1.5 (stat) +/- 4.2 dsyst__ MeV= c(2) and Gamma(pole) = (24: 8 +/- 3.3 (stat) +/- 11: 0 (syst)) MeV. The mass and width of the structure, which we refer to as Z(c)(3885), are 2 sigma and 1 sigma, respectively, below those of the Z(c)(3900) -> pi(+/-) J/psi peak observed by BESIII and Belle in pi(+)pi(-) J/psi final states produced at the same center- of- mass energy. The angular distribution of the pi Z(c)(3885) system favors a J(P) = J(P) = 1(+) quantum number assignment for the structure and disfavors 1(-) or 0(-). The Born cross section times the (D (D) over bar*) branching fraction of the Z(c)(3885) is measured to be sigma(e(+)e(-) -> pi(+/-)Z(c)(3885)(-/+)) x B(Z(c)(3885)-/+ -> (D (D) over bar*)(-/+) = (83.5 +/- 6.6 (stat) +/- 22.0 (syst)) pb. Assuming the Z(c)(3885) -> (D (D) over bar*)(-/+) signal reported here and the Z(c)(3900) -> pi J/psi signal are from the same source, the partial width ratio (Gamma(Z(c)(3885) -> D (D) over bar*)/Gamma(Z(c)(3900) -> pi J/psi)) = 6.2 +/- 1.1 (stat) +/- 2.7 (syst) is determined.
作者：
Liu, L.;Yang, M.;Kang, R.;Wang, Z.;Zhao, Y.;Yu, Y.;Xie, M.;Yin, X.;Livesey, K. M.;Lotze, M. T.;Tang, D.;Cao, L.
期刊：
Leukemia,2011年25(1):23-31 ISSN：0887-6924
通讯作者：
Cao, L
作者机构：
[Cao, L.; Xie, M.; Zhao, Y.; Wang, Z.; Yang, M.; Kang, R.; Liu, L.; Yu, Y.] Cent S Univ, Dept Pediat, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Livesey, K. M.; Lotze, M. T.; Tang, D.] Univ Pittsburgh, Dept Surg, Hillman Canc Ctr, Inst Canc, Pittsburgh, PA USA.;[Yin, X.] Nanhua Univ, Affiliated Hosp 1, Dept Pediat, Hengyang, Hunan, Peoples R China.
通讯机构：
[Cao, L] Cent S Univ, Dept Pediat, Xiangya Hosp, 110 Xiangya Rd Changsha, Changsha 410008, Hunan, Peoples R China.
关键词：
HMGB1;autophagy;PI3K;ERK;drug resistance
摘要：
Autophagy, a tightly regulated lysosome-dependent catabolic pathway, is important in the regulation of cancer development and progression and in determining the response of tumor cells to anticancer therapy. However, the role of autophagy in leukemia still remains largely unknown. Here we show that high-mobility group box 1 (HMGB1), the best characterized damage-associated molecular pattern, was released from leukemia cell lines after chemotherapy-induced cytotoxicity and activated autophagy to protect against injury. Treatment with HMGB1-neutralizing antibodies increased the sensitivity of leukemia cells to chemotherapy; whereas, exogenous HMGB1 rendered these cells more resistant to drug-induced cytotoxicity. Moreover, exogenous HMGB1 increased autophagy as evaluated by increased expression of the autophagic marker microtubule-associated protein light chain 3-II, degradation of sequestosome 1 (p62) and autophagosome formation. Furthermore, knockdown or pharmacological inhibition of either phosphoinositide 3-kinase-III or extracellular signal-regulated kinase kinase mitogen-activated protein kinase kinase/extra-cellular signal-regulated protein kinase inhibited HMGB1-induced autophagy. Taken together, these results suggest that HMGB1 release after chemotherapy is a critical regulator of autophagy and a potential drug target for therapeutic interventions in leukemia. Leukemia (2011) 25, 23-31; doi: 10.1038/leu.2010.225; published online 7 October 2010
作者：
Ablikim, M.;Achasov, M. N.;Albayrak, O.;Ambrose, D. J.;An, F. F.;An, Q.;Bai, J. Z.;Ferroli, R. Baldini;Ban, Y.;Becker, J.;Bennett, J. V.;Bertani, M.;Bian, J. M.;Boger, E.;Bondarenko, O.;Boyko, I.;Braun, S.;Briere, R. A.;Bytev, V.;Cai, H.
作者机构：
[Thorndike, E. H.; Muramatsu, H.; Ambrose, D. J.] Univ Rochester, Rochester, NY 14627 USA.;[Peters, K.; Goetzen, K.] GSI Helmholtzctr Heavy Ion Res GmbH, D-64291 Darmstadt, Germany.;[Bai, J. Z.; Li, W. D.; Dai, H. L.; Qin, X. S.; Zhu, K.; Liu, C. X.; Han, Y. L.; Ablikim, M.; Chen, J. C.; Zhao, J. W.; Qiu, J. F.; Ping, R. G.; Chen, G.; Liu, H.; He, K. L.; Zhang, J. Z.; Li, G.; Qin, Z. H.; Yang, H. X.; Song, W. M.; Tang, X.; Liu, H. W.; Jin, S.; Wang, L. S.; Zhang, J. Y.; Li, F.; Zhang, S. H.; Liao, X. T.; Liu, Zhiqing; Zhang, L.; Li, J. C.; Zhuang, J.; Li, H. B.; Ji, X. L.; Luo, X. L.; Chu, Y. P.; Wu, Z.; Yuan, Y.; Wang, B.; Zhu, S. H.; Zhao, G.; Wang, P. L.; Dai, J. P.; Gong, W. X.; Hu, H. M.; Ma, X. Y.; Dong, L. Y.; Lu, Y. P.; Gu, M. H.; Jiang, X. S.; Zhang, B. Y.; Li, X. N.; Zhu, Y. S.; Zheng, J. P.; Shen, X. Y.; Huang, L.; Zhao, Y. B.; Fang, J.; Wu, L. H.; Zhou, L.; Mo, X. H.; Cao, G. F.; Li, W. G.; Lai, W.; Zhang, X. J.; Zhang, B. X.; Jiang, L. L.; Wang, Z. G.; Zou, J. H.; Lou, X. C.; Zhang, Y.; Fu, C. D.; Sun, S. S.; Ye, H.; An, F. F.; Wang, Q. J.; Zhang, Y. H.; Wu, N.; Lv, M.; Cai, X.; Liu, Z. A.; Zhao, T. C.; Wang, Z. Y.; Chang, J. F.; Fang, S. S.; Zhu, C.; Dong, M. Y.; Ma, Q. M.; Deng, Z. Y.; Wang, P.; Qian, S.; Zhang, J. Q.; Ji, X. B.; Chen, H. S.; Zou, B. S.; Ma, S.; Song, X. Y.; Ouyang, Q.; Sheng, H. Y.; Xie, Y. G.; Ye, M.; Xiu, Q. L.; Jin, D. P.; Wang, L. L.; Ma, H. L.; Li, Q. J.; Liu, H. M.; Zhu, K. J.; Wang, Y. F.; Min, T. J.; Liu, B. J.; Sun, Y. Z.; Li, Lei; Rong, G.; Hou, Z. L.; Zhang, J. W.; Ji, Q.; Heng, Y. K.; Wang, K.; Xu, G. F.; Sun, D. H.; Zhu, Z. A.; Ma, T.; Chen, M. L.; Zhao, Ling; Zhang, H. Y.; Min, J.; Zhao, H. S.; Yuan, C. Z.; Li, C. H.; Zhang, D. H.; Sun, G. X.; Wen, S. P.; Zhang, C. C.; Ning, Z.; Xue, Z.; Sun, Z. J.; Liu, Fang; Wang, Z.; Hu, T.; He, M.; Chen, Y. B.; Liu, Zhiqiang; Lu, J. G.; Zhao, Q.; Mao, Z. P.; Yu, B. X.] Inst High Energy Phys, Beijing 100049, Peoples R China.;[Zafar, A. A.; Malik, Q. A.; Hussain, T.; Rashid, K. H.] Univ Punjab, Lahore 54590, Pakistan.;[Xia, L. G.; Zhu, X. L.; Liao, G. R.; Jing, F. F.; Liu, K.; Wang, X. F.; Gao, Y.] Tsinghua Univ, Beijing 100084, Peoples R China.
通讯机构：
[Ablikim, M] Inst High Energy Phys, Beijing 100049, Peoples R China.
关键词：
Beijing Electron Positron Collider - Center-of-mass energies - Data sample - Mass spectra - Reconstruction techniques
通讯机构：
[Xiao, ZQ] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
EGFR;HNSCC;Invasion;MMP‐9;E‐cadherin
摘要：
EGFR is a potent stimulator of invasion and metastasis in head and neck squamous cell carcinomas (HNSCC). However, the mechanism by which EGFR may stimulate tumor cell invasion and metastasis still need to be elucidated. In this study, we showed that activation of EGFR by EGF in HNSCC cell line SCC10A enhanced cell migration and invasion, and induced loss of epitheloid phenotype in parallel with downregulation of E-cadherin and upregulation of N-cadherin and vimentin, indicating that EGFR promoted SCC10A cell migration and invasion possibly by an epithelial to mesenchymal transition (EMT)-like phenotype change. Interestingly, activation of EGFR by EGF induced production of matrix metalloproteinase-9 (MMP-9) and soluble E-cadherin (sE-cad), and knockdown of MMP-9 by siRNA inhibited sE-cad production induced by EGF in SCC10A. Moreover, both MMP-9 knockdown and E-cadherin overexpression inhibited cell migration and invasion induced by EGF in SCC10A. The results indicate that EGFR activation promoted cell migration and invasion through inducing MMP-9-mediated degradation of E-cadherin into sE-cad. Pharmacologic inhibition of EGFR, MEK, and PI3K kinase activity in SCC10A reduced phosphorylated levels of ERK-1/2 and AKT, production of MMP-9 and sE-cad, cell migration and invasion, and expressional changes of EMT markers (E-cadherin and N-cadherin) induced by EGF, indicating that EGFR activation promotes cell migration and invasion via ERK1/2 and PI3K-regulated MMP-9/E-cadherin signaling pathways. Taken together, the data suggest that EGFR activation promotes HNSCC SCC10A cell migration and invasion by inducing EMT-like phenotype change and MMP-9-mediated degradation of E-cadherin into sE-cad related to activation of ERK-1/2 and PI3K signaling pathways. J. Cell. Biochem. 112: 2508-2517, 2011. (C) 2011 Wiley-Liss, Inc.
作者：
Shuibo, Xie*;Chun, Zhang;Xinghuo, Zhou;Jing, Yang;Xiaojian, Zhang;Jingsong, Wang
期刊：
Journal of environmental radioactivity,2009年100(2):162-166 ISSN：0265-931X
通讯作者：
Shuibo, Xie
作者机构：
[Jingsong, Wang; Shuibo, Xie; Jing, Yang; Xinghuo, Zhou; Chun, Zhang] School of Urban Construction, University of South China, Hengyang, Hunan 421001, China;[Shuibo, Xie; Xiaojian, Zhang] Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, China
通讯机构：
[Xie, SB] Univ S China, Sch Urban Construct, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
BACKGROUND: The pathogenesis of nasopharyngeal carcinoma (NPC) is a complicated process involving genetic predisposition, Epstein-Bar Virus infection, and genetic alterations. Although some oncogenes and tumor suppressor genes have been previously reported in NPC, a complete understanding of the pathogenesis of NPC in the context of global gene expression, transcriptional pathways and biomarker assessment remains to be elucidated. METHODS: Total RNA from 32 pathologically-confirmed cases of poorly-differentiated NPC was divided into pools inclusive of four consecutive specimens and each pool (T1 to T8) was co-hybridized with pooled RNA from 24 normal non-cancerous nasopharyngeal tissues (NP) to a human 8K cDNA array platform. The reliability of microarray data was validated for selected genes by semi-quantitative RT-PCR and immunohistochemistry. RESULTS: Stringent statistical filtering parameters identified 435 genes to be up-regulated and 257 genes to be down-regulated in NPC compared to NP. Seven up-regulated genes including CYC1, MIF, LAMB3, TUBB2, UBE2C and TRAP1 had been previously proposed as candidate common cancer biomarkers based on a previous extensive comparison among various cancers and normal tissues which did not, however, include NPC or NP. In addition, nine known oncogenes and tumor suppressor genes, MIF, BIRC5, PTTG1, ATM, FOXO1A, TGFBR2, PRKAR1A, KLF5 and PDCD4 were identified through the microarray literature-based annotation search engine MILANO, suggesting these genes may be specifically involved in the promotion of the malignant conversion of nasopharyngeal epithelium. Finally, we found that these differentially expressed genes were involved in apoptosis, MAPK, VEGF and B cell receptor signaling pathways and other functions associated with cell growth, signal transduction and immune system activation. CONCLUSION: This study identified potential candidate biomarkers, oncogenes/tumor suppressor genes involved in several pathways relevant to the oncogenesis of NPC. This information may facilitate the determination of diagnostic and therapeutic targets for NPC as well as provide insights about the molecular pathogenesis of NPC.
期刊：
Chemical communications (Cambridge, England),2011年47(33):9516-9518 ISSN：1359-7345
通讯作者：
Xiao, JC
作者机构：
[Zheng, Xing; Wang, Xiao-Ping] Univ S China, Inst Pharm & Pharmacol, Hengyang, Hunan, Peoples R China.;[Zhang, Cheng-Pan; Cai, Ji; Wang, Xiao-Ping; Xiao, Ji-Chang; Zhou, Chang-Bing] Chinese Acad Sci, Shanghai Inst Organ Chem, Key Lab Organofluorine Chem, Shanghai 200032, Peoples R China.;[Gu, Yu-Cheng] Syngenta, Jealotts Hill Int Res Ctr, Bracknell RG42 6EY, Berks, England.
通讯机构：
[Xiao, JC] Chinese Acad Sci, Shanghai Inst Organ Chem, Key Lab Organofluorine Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China.
摘要：
The ligand-free trifluoromethylation of arylboronic acids with a [Ph(2)SCF(3)](+)[OTf](-)/Cu(0) system has been carefully investigated. Aryl-, alkenyl- and heteroarylboronic acids with a variety of functional groups were suitable substrates for this reaction. It is suggested that a CuCF(3) species is formed under the reaction conditions.
期刊：
Journal of Biological Chemistry,2008年283(6):3418-3423 ISSN：0021-9258
通讯作者：
Cao, DL
作者机构：
[Rao, Krishna; Cheng, Ji-Ming] Division of Hematology/Oncology, Internal Medicine, Southern Illinois University School of Medicine, Springfield, IL 62702, United States;[Zu, Xuyu; Liao, Duan-Fang] Division of Pharmacoproteomics, Institute of Pharmacy and Pharmacology, Nanhua University School of Life Science and Technology, 28 Changshengxi Road, Hengyang, Hunan 421001, China;[Cao, Deliang] Dept. of Medical Microbiology, Immunology, and Cell Biology, SimmonsCooper Cancer Inst., Southern Illinois University School of Medicine, 913 N. Rutledge St., Springfield, IL 62702, United States;[Cao, Deliang; Zu, Xuyu; Ma, Jun; Rao, Krishna; Yan, Ruilan] Department of Medical Microbiology, Immunology, and Cell Biology, SimmonsCooper Cancer Institute, Southern Illinois University School of Medicine, Springfield, IL 62702, United States
通讯机构：
[Cao, DL] So Illinois Univ, Sch Med, SimmonsCooper Canc Inst, Dept Med Microbiol, 913 N Rutledge St, Springfield, IL 62702 USA.
关键词：
Aldo-keto reductases - Breast cancer cells - Cancer cell growths - Colocalize - Essential components - Fatty acid syntheses - Gel filtrations - Hepatocellular carcinomata - Immunoprecipitation - Ion exchange chromatographies - Lung carcinomata - Mammary epithelial cells - Mass spectrometry analysis - Novel functions - Novel proteins - Proteasome - Protein complexes - Pulldown assays - Sepharose - Small interfering RNAs - Ubiquitination
期刊：
Journal of cell science,2011年124(17):2997-3005 ISSN：0021-9533
通讯作者：
Ma, J
作者机构：
[Li, Guiyuan; Zeng, Xi; Deng, Min; Tang, Hailin; Liao, Qianjin; Zhou, Yanhong; Xiong, Wei; Guo, Xiaofang; Zheng, Ying; Zhou, Ming; Ma, Jian; Li, Xiaoling; Ye, Qiurong] Cent S Univ, Canc Res Inst, Changsha 410078, Hunan, Peoples R China.;[Zeng, Xi; Deng, Min; Tang, Hailin; Liao, Qianjin] Univ S China, Canc Res Inst, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Ma, J] Cent S Univ, Canc Res Inst, 110 Xiang Ya Rd, Changsha 410078, Hunan, Peoples R China.
关键词：
microRNA-216b;KRAS;Nasopharyngeal carcinoma
摘要：
MicroRNAs (miRNAs) are small noncoding RNAs that are involved in various diseases, including cancer. In the present study, we found that miR-216b was downregulated in nasopharyngeal carcinoma (NPC) cell lines and specimens. Decreased expression of miR-216b was directly related to advanced clinical stage and lymph node metastasis. miR-216b levels correlated inversely with levels of KRAS protein during nasopharyngeal tumorigenesis. Furthermore, we demonstrated that miR-216b can bind to the 3' untranslated region (UTR) of KRAS and inhibit expression of the KRAS protein. Both in vitro and in vivo assays revealed that miR-216b attenuated NPC cell proliferation, invasion and tumor growth in nude mice. miR-216b exerts its tumor suppressor function through inhibition of the KRAS-related AKT and ERK pathways. Our findings provide, for the first time, significant clues regarding the role of miR-216b as a tumor suppressor by targeting KRAS in NPC.
作者机构：
[Shunlin Qu; Meidong Liu; Honglin Zhu; Chi Zhang; Guangwen Chen; Qi Luo; Yansheng Feng; Xianzhong Xiao; Xing Wei] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410078, Hunan, Peoples R China.;[Shunlin Qu; Chi Zhang; Xing Wei] Univ S China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, XZ] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, 110 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.
关键词：
Resveratrol;Doxorubicin;SIRT1;p53;Apoptosis
摘要：
AIMS: Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of clinical antineoplastic activity, but increased apoptosis has been implicated in its cardiotoxicity. Resveratrol (RES) was shown to harbour major health benefits in diseases associated with oxidative stress. In this study, we aimed to determine the effect of RES on DOX-induced myocardial apoptosis in mice. METHODS AND RESULTS: Male Balb/c mice were randomized to one of the following four treatments: saline, RES, DOX, or RES plus DOX (10 mice in each group). DOX treatment markedly depressed cardiac function, decreased the heart weight, the body weight, and the ratio of heart weight to body weight, but inversely increased the level of protein carbonyl, malondialdehyde, and serum lactate dehydrogenase, and induced mitochondrial cytochrome c release and cardiomyocyte apoptosis. However, these effects of DOX were ameliorated by its combination with RES. Further studies with a co-immunoprecipitation assay revealed an interaction between p53 and Sirtuin 1 (SIRT1). It was found by western blot and electrophoretic mobility shift assay that DOX treatment increased p53 protein acetylation and cytochrome c release from mitochondria, activated p53 binding at the Bax promoter, and up-regulated Bax expression, but supplementation with RES could weaken all these effects. CONCLUSION: The protective effect of RES against DOX-induced cardiomyocyte apoptosis is associated with the up-regulation of SIRT1-mediated p53 deacetylation.
作者：
Xie, Shuibo;Yang, Jing;Chen, Chao;Zhang, Xiaojian;Wang, Qingliang;Zhang, Chun
期刊：
Journal of environmental radioactivity,2008年99(1):126-133 ISSN：0265-931X
通讯作者：
Xie, S.(xiesbmr@263.net)
作者机构：
[ Yang, Jing ; Wang, Qingliang ; Xie, Shuibo ; Zhang, Chun ] School of Urban Construction, University of South China, Hengyang, Hunan 421001, China;[ Chen, Chao ; Xie, Shuibo ; Zhang, Xiaojian ] Department of Environment Science and Engineering, Tsinghua University, Beijing, 100084, China
通讯机构：
[Xie, Shuibo] Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, P R China.
摘要：
Biosorption has been developed as an effective and economic method to treat wastewater containing low concentrations of metal pollutants. In this study, a bacterium, Citrobacter freudii, was used as a biosorbent to adsorb uranium ions. The thermodynamics and kinetics of this adsorption, as well as its mechanism, were investigated. The results indicated that the biosorption rate could be better described by a pseudo 2nd-order model than a pseudo 1st-order model. The adsorption of U (VI) proceeded very rapidly in the first 30 min and subsequently slowed down continuously for a long period. The biosorption isotherm of uranium by C. freudii could be described well by the Langmuir or Freundlich isotherm, and the latter was better. The thermodynamics parameters, Delta H degrees, Delta G degrees, and Delta S degrees were calculated according to the results of the experiment, which showed this biosorption as being endothermic and spontaneous. The authors investigated the active sites of bacteria for biosorption and the results proved that carboxyl in the cell wall played an important role in biosorption. (c) 2007 Elsevier Ltd. All rights reserved.
期刊：
MOLECULAR MEDICINE REPORTS,2012年5(3):753-760 ISSN：1791-2997
通讯作者：
Wang, XC
作者机构：
[Long, Yu; Guo, Xiaofang; Liu, Meiling; Huang, Chunxia; Su, Min; Tian, Zhi; Liu, Lipeng; Zhou, Feng] Changsha Med Univ, Dept Biochem & Mol Biol, Changsha 410219, Hunan, Peoples R China.;[Wu, Xinhua] Cent S Univ, Xiangya Hosp, Dept Obstet & Gynecol, Changsha 410078, Hunan, Peoples R China.;[Wang, Xiaochun] Cent S Univ, Dept Med Lab Examinat, Changsha 410013, Hunan, Peoples R China.;[Yu, Xiaohua] Nanhua Univ, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, XC] Cent S Univ, Dept Med Lab Examinat, Changsha 410013, Hunan, Peoples R China.
关键词：
R-143;Bcl-2;He La;proliferation;apoptosis
摘要：
microRNAs (miRNAs) are small non-coding RNA molecules of 21-24 nt that regulate the expression of other genes by transcriptional inhibition or translational repression. Multiple lines of evidence suggest that miRNAs play important roles in tumor development and progression. We identified 24 mi RNAs markedly and aberrantly expressed in human cervical cancer. The most significantly deregulated was miR-143 as determined by miRNA microarray analysis. miR-143 was introduced into He La cells and it was found that the overexpression of miR-143 significantly inhibited He La cell proliferation and promoted apoptosis; anti-miR-143 rescued the effects. He La cells transfected with pre-miR-143, pre-anti-miR-143 or control mi RNA precursor were injected subcutaneously into the flanks of female athymic nude mice, and the overexpression of miR-143 suppressed the formation of tumors. Compared with normal cervical tissues, the levels of Bcl-2 were increased in miR-143-downregulated tissues. Sustained overexpression of miR-143 in He La cells resulted in suppression of Bcl-2 expression, and knockdown of miR-143 by anti-miR-143 increased Bcl-2 expression. In addition, overexpression of Bcl-2 partially reversed the inhibition of proliferation and promotion of apoptosis in the He La cells caused by miR-143. Furthermore, miR-143 suppressed the activity of a luciferase reporter carrying the 3'-UTR of Bcl-2, which was abolished by mutation of the predicted miR-143-binding site, indicating that Bcl-2 is a miR-143 target gene. Our study revealed a molecular link between miR-143 and Bcl-2. Direct involvement in the regulation of Bcl-2 may be one of the mechanisms through which mi R-143 may play a role in the pathogenesis of cervical cancer.
