作者机构:
[Zhang, Qi; Lu, Yiming; Lu, Hao; Zhao, Xi; Hao, Rongjiao; Han, Huihui; Li, Yuanfeng; Zhou, Gangqiao; Jiang, Siao; Xing, Shuang; Quan, Cheng; Chen, Hongxia; Yu, Zuyin] Beijing Inst Radiat Med, Beijing Proteome Res Ctr, Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing, Beijing, Peoples R China.;[Wang, Qianqian; Yang, Wei] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Radiat Oncol, Beijing, Peoples R China.;[Zhang, Qi; Zhou, Gangqiao] Univ South China, Sch Med, Hengyang, Hunan, Peoples R China.;[Lu, Yiming; Hao, Rongjiao; Zhou, Gangqiao; Jiang, Siao] Univ Hebei, Sch Life Sci, Baoding, Hebei, Peoples R China.;[Feng, Changjiang] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Thorac Surg, Beijing, Peoples R China.
通讯机构:
[Zhou, GQ ; Lu, YM] B;Beijing Inst Radiat Med, Beijing Proteome Res Ctr, Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing, Beijing, Peoples R China.;Univ South China, Sch Med, Hengyang, Hunan, Peoples R China.;Univ Hebei, Sch Life Sci, Baoding, Hebei, Peoples R China.;Nanjing Med Univ, Sch Publ Hlth, Collaborat Innovat Ctr Personalized Canc Med, Ctr Global Hlth, Nanjing, Jiangsu, Peoples R China.
摘要:
Radiation triage and biological dosimetry are critical for the medical management of massive potentially exposed individuals following radiological accidents. Here, we performed a genome-wide screening of radiation-responding mRNAs, whose N6-methyladenosine (m(6)A) levels showed significant alteration after acute irradiation. The m(6)A levels of three genes, Ncoa4, Ate1 and Fgf22, in peripheral blood mononuclear cells (PBMCs) of mice showed excellent dose-response relationships and could serve as biomarkers of radiation exposure. Especially, the RNA m(6)A of Ncoa4 maintained a high level as long as 28 days after irradiation. We demonstrated its responsive specificity to radiation, conservation across the mice, monkeys and humans, and the dose-response relationship in PBMCs from cancer patients receiving radiation therapy. Finally, NOCA4 m(6)A-based biodosimetric models were constructed for estimating absorbed radiation doses in mice or humans. Collectively, this study demonstrated the potential feasibility of RNA m(6)A in radiation accidents management and clinical applications. Radiation dosimetry are critical for the medical management of individuals exposed to ionizing radiation (IR). Here, authors show that the RNA m6A levels of Ncoa4, Ate1 and Fgf22 genes in peripheral blood cells could serve as dosimetry of IR exposure.
期刊:
JMIR Public Health and Surveillance,2023年9(1):e49291- ISSN:2369-2960
通讯作者:
Wang, J
作者机构:
[Wang, Jian; Guo, Qulian; Song, Zongbin; Hou, Xinran; Zhu, Maoen; Wang, J] Cent South Univ, Xiangya Hosp, Dept Anesthesiol, 87 Xiangya Rd, Changsha 410008, Peoples R China.;[Wang, Jian; Guo, Qulian; Song, Zongbin; Zhang, Chengliang; Hou, Xinran; Zhu, Maoen] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Peoples R China.;[Xu, Wei] Univ South China, Hunan Prov Maternal & Child Hlth Care Hosp, Dept Anesthesiol, Changsha, Peoples R China.;[Zhang, Chengliang] Cent South Univ, Xiangya Hosp, Dept Cardiovasc Surg, Changsha, Peoples R China.
通讯机构:
[Wang, J ] C;Cent South Univ, Xiangya Hosp, Dept Anesthesiol, 87 Xiangya Rd, Changsha 410008, Peoples R China.
关键词:
serum chloride;all-cause mortality;cause-specific mortality;National Health and Nutrition Examination Survey;National Death Index
摘要:
BACKGROUND: Chloride is the most abundant anion in the human extracellular fluid and plays a crucial role in maintaining homeostasis. Previous studies have demonstrated that hypochloremia can act as an independent risk factor for adverse outcomes in various clinical settings. However, the association of variances of serum chloride with long-term mortality risk in general populations has been rarely investigated. OBJECTIVE: This study aims to assess the association of serum chloride with all-cause and cause-specific mortality in the general American adult population. METHODS: Data were collected from 10 survey cycles (1999-2018) of the National Health and Nutrition Examination Survey. All-cause mortality, cardiovascular disease (CVD) mortality, cancer mortality, and respiratory disease mortality data were obtained by linkage to the National Death Index through December 31, 2019. After adjusting for demographic factors and relevant lifestyle, laboratory items, and comorbid factors, weighted Cox proportional risk models were constructed to estimate hazard ratios and 95% CIs for all-cause and cause-specific mortality. RESULTS: A total of 51,060 adult participants were included, and during a median follow-up of 111 months, 7582 deaths were documented, 2388 of CVD, 1639 of cancer, and 567 of respiratory disease. The weighted Kaplan-Meier survival analyses showed consistent highest mortality risk in individuals with the lowest quartiles of serum chloride. The multivariate-adjusted hazard ratios from lowest to highest quartiles of serum chloride (≤101.2, 101.3-103.2, 103.2-105.0, and ≥105.1 mmol/L) were 1.00 (95% CI reference), 0.77 (95% CI 0.67-0.89), 0.72 (95% CI 0.63-0.82), and 0.77 (95% CI 0.65-0.90), respectively, for all-cause mortality (P for linear trend<.001); 1.00 (95% CI reference), 0.63 (95% CI 0.51-0.79), 0.56 (95% CI 0.43-0.73), and 0.67 (95% CI 0.50-0.89) for CVD mortality (P for linear trend=.004); 1.00 (95% CI reference), 0.67 (95% CI 0.54-0.84), 0.65 (95% CI 0.50-0.85), and 0.65 (95% CI 0.48-0.87) for cancer mortality (P for linear trend=.004); and 1.00 (95% CI reference), 0.68 (95% CI 0.41-1.13), 0.59 (95% CI 0.40-0.88), and 0.51 (95% CI 0.31-0.84) for respiratory disease mortality (P for linear trend=.004). The restricted cubic spline analyses revealed the nonlinear and L-shaped associations of serum chloride with all-cause and cause-specific mortality (all P for nonlinearity<.05), in which lower serum chloride was prominently associated with higher mortality risk. The associations of serum chloride with mortality risk were robust, and no significant additional interaction effect was detected for all-cause mortality and CVD mortality (P for interaction>.05). CONCLUSIONS: In American adults, decreased serum chloride concentrations were independently associated with increased all-cause mortality, CVD mortality, cancer mortality, and respiratory disease mortality. Our findings suggested that serum chloride may serve as a promising cost-effective health indicator in the general adult population. Further studies are warranted to explore the potential pathophysiological mechanisms underlying the association between serum chloride and mortality.
作者机构:
[Ye, Jiawei; Liu, Shuang; Cai, Jintao] Univ South China, Coll Mech Engn, Hengyang 421200, Peoples R China.;[Wu, Peiwei; Zhao, Lihong] Univ South China, Coll Nucl Sci & Technol, Hengyang 421200, Peoples R China.
通讯机构:
[Zhao, LH ] U;Univ South China, Coll Nucl Sci & Technol, Hengyang 421200, Peoples R China.
关键词:
cutting path calculation;end cutting;machine vision;peripheral identification;precision control
摘要:
Abstract: In response to the lack of plasma bag-breaking equipment and time-consuming manual recovery of plasma in China, this study focuses on the distal end cutting of a plasma bag residual-input tube and designs a machine-vision-based plasma bag residual-input tube cutting system. The system comprises a plasma bag rotation device, a bottom disc, an image acquisition device, a cutting device, and a device control system. The image acquisition device captures images of the rotating plasma bag and calculates the Euclidean distance from the end of the plasma bag input-tube distal end to the outer edge of the bottom support plate and the distance from the input-tube distal end to the centerline of the plasma bag. Two distinct value acquisition algorithms are designed for the two types of input tubes. The screw slide moves accordingly. When the plasma bag is above the cutting part, the cutting part cuts the end of the residual-input tube of the plasma bag. The prototype performance tests reveal that the cutting error is within 10% of the end of the plasma bag input tube distal end, the cutting success rate is 100%, and the processing time for each bag is 2.5 s, which meets the time requirements of the plasma bag breaker. Keywords: machine vision; peripheral identification; precision control; end cutting; cutting path calculation
摘要:
跨语言影响在二语学习研究中是个经典话题。《跨语言影响与二语学习》(Crosslinguistic Influence and Second Language Learning,2021)一书对二语学习和跨语言概念、理论模型和教学实践做出了全面、客观、完整的总结与说明。该书的研究范围覆盖面广,信息量大,文字简明易懂,视角新奇独到,实用性强,适用性广泛,对我国二语的发展有着很大的参考价值与指导意义。
关键词:
axillary osmidrosis;endoscopic assistance;negative pressure suction;rotary cutter;skin surgery
摘要:
BACKGROUND: Bromhidrosis, also known as body odor, is a common disease in life, which often occurs in young adults. The histological basis of bromhidrosis is the hyperplasia of apocrine sweat glands. OBJECTIVE: To compare the effects of different methods of endoscopy in microdynamic axillary osmidrosis removal on curative effect, complications, and surgical efficiency. METHODS: A total of 149 patients with axillary osmidrosis were treated in our hospital from January 2020 to December 2021. They were treated with endoscopic assistance in the whole process of operation (Group A) and endoscope-assisted exploration after blind rotary cutter suction (Group B), respectively, and the curative effect, complication rate, and surgical efficiency were evaluated. RESULTS: There was no significant difference in the curative effect and complication rate between the two groups, but the endoscope-assisted exploration group after suction with rotary cutter (Group B) had higher surgical efficiency. CONCLUSION: On the basis of professional use of rotary cutter, it is efficient to choose endoscope to check the excision of sweat gland in the operation area and stop bleeding in time after blind suction.
期刊:
Molecular and Cellular Biochemistry,2023年 ISSN:0300-8177
通讯作者:
Jiang, Zhisheng
作者机构:
[He, Shuya; He, Siqi; Jiang, Zhisheng; Ma, Yun; Gu, Tianhe] Univ South China, Inst Biochem & Mol Biol, Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhisheng; Ma, Yun] Univ South China, Inst Cardiovasc Dis, Hengyang Med Sch, Key Lab Arteriosclerol Hunan Prov,Hunan Int Sci &, Hengyang 421001, Hunan, Peoples R China.;[Ma, Yun] Univ South China, Hengyang Med Sch, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Jiang, Zhisheng] I;Institute of Biochemistry and Molecular Biology, Hengyang Medical College, University of South China, Hengyang, 421001, Hunan, China.;Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, Hengyang Medical School, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, University of South China, Hengyang, 421001, Hunan, China.
摘要:
Cardiovascular disease (CVD) has a high incidence and low cure rate worldwide, and atherosclerosis (AS) is the main factor inducing cardiovascular disease, of which lipid deposition in the vessel wall is the main marker of AS. Currently, although statins can be used to lower lipids and low-density lipoprotein (LDL) in AS, the cure rate for AS remains low. Therefore, there is an urgent need to develop new therapeutic approaches, and stem cells are now widely studied, while stem cells are a class of cell types that always maintain the ability to differentiate and can differentiate to form other cells and tissues, and stem cell transplantation techniques have shown efficacy in the treatment of other diseases. With the establishment of cellular therapies and continued research in stem cell technology, stem cells are also being used to address the problem of AS. In this paper, we focus on recent research advances in stem cell therapy for AS and briefly summarize the relevant factors that induce the formation of AS. We mainly discuss the efficacy and application prospects of mesenchymal stem cells (MSCs) for the treatment of AS, in addition to the partial role and potential of exosomes in the treatment of AS. Further, provide new ideas for the clinical application of stem cells.
期刊:
Combinatorial Chemistry & High Throughput Screening,2023年 ISSN:1386-2073
作者机构:
[Xiang, Yijun; Yang, Zehua; Wang, Yanjie; Mi, Pengbing; Yao, Xu; Ai, Wenbin; Li, Jiaxin; Li, Xiaoshun; Zheng, Zitong; Jiang, Jingyi] Department of Pharmacy, Hunan Provincial Key Laboratory of tumor microenvironment responsive drug research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, the Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China
摘要:
Resveratrol is one of the most interesting naturally-occurring nonflavonoid phenolic compounds with various biological activities, such as anticancer, neuroprotection, antibacterial, and anti-inflammatory. However, there is no clinical usage of resveratrol due to either its poor activity or poor pharmacokinetic properties. Heteroarenes-modified resveratrol is one pathway to improve its biological activities and bioavailability, and form more modification sites. In this review, we present the progress of heteroaryl analogues of resveratrol with promising biological activities in the latest five years, ranging from the synthesis to the structure-activity relationship and mechanism of actions. Finally, introducing heteroarenes into resveratrol is an effective strategy, which focuses on the selectivity of structure-activity relationship in vivo.
关键词:
Anti-breast cancer activities;Baker-Venkataraman reaction;Fluorinated-genistein derivatives;Genistein;MTT assay;structure-activity relationship
摘要:
Background: Genistein has been limited in clinical application due to its low bioavailability, extremely poor liposolubility, and fast glycosylation rate, though it possesses anti-breast cancer activity. Therefore, the discovery of novel genistein derivatives is an urgency.<&wdkj&>Objective: To enhance the anti-breast cancer activity of genistein, a series of novel fluorinated genistein derivatives were synthesized.<&wdkj&>Methods: Their in vitro antitumor activity was investigated by the MTT assay against three cancer cell lines, via, MDA-MB-231, MCF-7, and MDA-MB-435, respectively.<&wdkj&>Results: Analogs 1d, 2b, and 3b showed remarkable anticancer activities compared to tamoxifen, a clinical anti-breast cancer drug on the market.<&wdkj&>Conclusion: The activities against breast cancer of genistein were enhanced by introducing the 7- alkoxyl group and fluorine atom into the B-ring. Therefore, these compounds may be potential candidates for treating breast cancer.
期刊:
Infection and Drug Resistance,2023年16(Volume 16, 2023 - Issue):3157-3169 ISSN:1178-6973
通讯作者:
Yuan, XQ;Li, GL
作者机构:
[Yuan, Xiuqin; Yu, Jinjie; Yuan, XQ] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Deng, Lele; Zhao, Xiuqin; Liu, Haican; Li, Guilian; Yu, Jinjie; Wan, Kanglin] Natl Inst Communicable Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Dis, Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China.;[Xiao, Hui; Yin, Chunjie; Liu, Mengwen; Li, Machao] Xinjiang Med Univ, Sch Publ Hlth, Urumqi 830011, Xinjiang, Peoples R China.;[Xu, Miao; Mijiti, Xiaokaiti; Wang, Quan] Xinjiang Med Univ, Affiliated Hosp 8, Urumqi 830049, Xinjiang, Peoples R China.;[Anwaierjiang, Aiketaguli] Coll Xinjiang Uyghur Med, Hetian 848000, Peoples R China.
通讯机构:
[Li, GL ] N;[Yuan, XQ ] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Natl Inst Communicable Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Dis, Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China.
关键词:
vitamin D receptor;VDR;polymorphism;tuberculosis;Mycobacterium tuberculosis;Beijing lineage
摘要:
BACKGROUND: The aim of the present study was to investigate the association between vitamin D receptor (VDR) gene polymorphism and tuberculosis susceptibility, as well as the potential interaction of host genetic factors with the heterogeneity of Mycobacterium tuberculosis in the population from Xinjiang, China. METHODS: From January 2019 to January 2020, we enrolled 221 tuberculosis patients as the case group and 363 staff with no clinical symptoms as the control group from four designated tuberculosis hospitals in southern Xinjiang, China. The polymorphisms of Fok I, Taq I, Apa I, Bsm I, rs3847987 and rs739837 in the VDR were detected by sequencing. M. tuberculosis isolates were collected from the case group and identified as Beijing or non-Beijing lineage by multiplex PCR. Propensity score (PS), univariate analysis and multivariable logistic regression models were used to perform the analysis. RESULTS: Our results showed that the allele and genotype frequencies of Fok I, Taq I, Apa I, Bsm I, rs3847987 and rs739837 in VDR were not correlated with tuberculosis susceptibility or lineages of M. tuberculosis. Two out of six loci of the VDR gene formed one haplotype block, and none of the haplotypes was found to correlate with tuberculosis susceptibility or lineages of M. tuberculosis infected. CONCLUSION: Polymorphisms in the VDR gene may not indicate susceptibility to tuberculosis. There was also no evidence on the interaction between the VDR gene of host and the lineages of M. tuberculosis in the population from Xinjiang, China. Further studies are nonetheless required to prove our conclusions.
期刊:
Journal of Healthcare Engineering,2023年2023 ISSN:2040-2295
作者机构:
[Xu, Zhun] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Spine Surg, Hengyang 421000, Hunan Province, Peoples R China.;[Li, Yikai; Zhang, Zhaocong; Xu, Zhun; Feng, Ziyu] Southern Med Univ, Sch Tradit Chinese Med, 1838 Guangzhou Ave North, Guangzhou 510515, Guangdong Provi, Peoples R China.;[Zhang, Kunmu] Fujian Univ Tradit Chinese Med, Affiliated Hosp 2, 282 Wusi Rd, Fuzhou 350003, Fujian Province, Peoples R China.
摘要:
Objective. Clinical studies have found that manipulation of oblique pulling has a good clinical effect on sacroiliac joint pain. However, there is no uniform standard for manipulation of oblique pulling at present. The purpose of this study was to investigate the effects of four manipulations of oblique pulling on sacroiliac joint and surrounding ligaments. Methods. A three-dimensional finite element model of the pelvis was established. Four manipulations of oblique pulling were simulated. The stresses and displacements of sacroiliac joint and the strains of surrounding ligaments were analyzed under four manipulations of oblique pulling. Results. Manipulation of oblique pulling F2 and F3 caused the highest and lowest stress on the pelvis, at 85.0 and 52.6 MPa, respectively. Manipulation of oblique pulling F3 and F1 produced the highest and lowest stress on the left sacroiliac joint, at 6.6 and 5.6 MPa, respectively. The four manipulations of oblique pulling mainly produced anterior-posterior displacement. The maximum value was 1.21 mm, produced by manipulation of oblique pulling F2, while the minimal value was 0.96 mm, produced by manipulation of oblique pulling F3. The four manipulations of oblique pulling could all cause different degrees of ligament strain, and manipulation of oblique pulling F2 produced the greatest ligament strain. Conclusions. The four manipulations of oblique pulling all produced small displacements of sacroiliac joint. However, they produced different degrees of ligament strain. Manipulation of oblique pulling F2 produced the largest displacement of sacroiliac joint and the greatest ligament strain, which could provide a certain reference for physiotherapists.
作者机构:
[Sun, Xingyao; Tan, Jinpeng; Zhou, Ping-Kun] Hengyang Medical College University of South China Hengyang Hunan Province China;[Zhao, Hongling; Guan, Hua; Sun, Xingyao; Tan, Jinpeng; Gao, Shanshan; Zhou, Ping-Kun] Department of Radiation Biology Beijing Key Laboratory for Radiobiology Beijing Institute of Radiation Medicine Beijing China
摘要:
Double‐strand break (DSB) is a critical DNA damage induced by ionizing radiation, and which is an actuating signal triggering cancer cell death in tumor radiotherapy. In mammalian cells, nonhomologous end‐joining (NHEJ) and homologous recombination (HR) are the two main pathways of DNA DSB repair, and both cooperate and compete with each other to promote efficient repair. Many regulatory factors play essential roles in the DSB repair pathway. For example, the MRE11–RAD50–NBS1 (MRN) complex and the Ku70/80 heterodimer/DNA–PKcs (DNA–PK) complex are essential for DSB recognition and recruitment of DNA repair factors, and their regulation plays an important role in the choice of the DSB repair HR and NHEJ pathways. Studies and achievements on the molecular mechanisms of the DSB repair pathway may facilitate the development of proteins‐or pathway‐specific inhibitors and advance targeted precise therapy of cancers, and also improving the efficacy of cancer radiotherapy. Here, we review the structures, functions and molecular mechanisms, and inhibitors of key target proteins in the DSB repair pathways. The strategy and application in cancer therapy are also discussed based on the advancement of inhibitors targeted DSB repair proteins. Abstract Double‐strand break (DSB), a significant DNA damage brought on by ionizing radiation, acts as an initiating signal in tumor radiotherapy, causing cancer cells death. The two primary pathways for DNA DSB repair in mammalian cells are nonhomologous end joining (NHEJ) and homologous recombination (HR), which cooperate and compete with one another to achieve effective repair. The DSB repair mechanism depends on numerous regulatory variables. DSB recognition and the recruitment of DNA repair components, for instance, depend on the MRE11–RAD50–NBS1 (MRN) complex and the Ku70/80 heterodimer/DNA–PKcs (DNA–PK) complex, whose control is crucial in determining the DSB repair pathway choice and efficiency of HR and NHEJ. In‐depth elucidation on the DSB repair pathway's molecular mechanisms has greatly facilitated for creation of repair proteins or pathways‐specific inhibitors to advance precise cancer therapy and boost the effectiveness of cancer radiotherapy. The architectures, roles, molecular processes, and inhibitors of significant target proteins in the DSB repair pathways are reviewed in this article. The strategy and application in cancer therapy are also discussed based on the advancement of inhibitors targeted DSB damage response and repair proteins.