期刊论文

Schistosoma japonicum exogenous short peptide on macrophage polarization in mice

中文

中南医学科学杂志

2095-1116

2019

47

2

120-124,138

10.15972/j.cnki.43-1509/r.2019.02.002

湖南省自然科学基金(2016JJ2116).

本校为第一机构

日本血吸虫肝硬化与宿主巨噬细胞极化密切相关,本研究为验证多肽干预宿主巨噬细胞极化的分子机制,人工培养小鼠巨噬细胞,分别用单磷酸腺苷、日本血吸虫碱性磷酸酶、外源性短肽处理,ELISA检测各试剂最佳浓度;免疫印迹法检测短肽作用后细胞内一氧化氮合酶以及精氨酸酶1蛋白含量变化;酶联免疫吸附法检测细胞上清液中腺苷浓度的改变,对硝基苯磷酸二钠法检测日本碱性磷酸酶酶活,实时荧光定量PCR法检测细胞上清液中一氧化氮合酶、精氨酸酶1 mRNA的表达变化,硝酸还原酶法测定一氧化氮的浓度,免疫荧光技术鉴别M1和M2型巨噬细胞。结果显示,单磷酸腺苷、碱性磷酸酶及短肽最佳浓度分别为2. 5 mmol/L、1. 1 ...

Schistosoma japonicum cirrhosis is closely related to macrophage polarization. In order to verify the molecular mechanism of ZLW intervention polarization, RAW264.7 cells was artificially cultured, and treated with adenosine monophosphate, Schistosoma japonicum alkaline phosphatase, exogenous short peptide, ELISA to determine the optimal concentration of each reagent;Western Blot was used to detect changes of nitric iNOS and Arg1 protein in cells after treatment with ZLW. ELISA was used to detect changes in adenosine concentration in cell supernatants. The activity of Japanese alkaline phospha...