BioTechnology: An Indian Journal,2013年8(4):543-550 ISSN：0974-7435
[Tuo, Qin-Hui; Liao, Duan-Fang; Gong, Yong-Zhen; Sun, Shao-Wei] Hunan University of Chinese Medicine, Hanpu Science and Education District, 1# Xiangzui Road, Changsha 410208, Hunan, China;[Ke-Tang, Chao] Institute of Cardiovascular Disease, Life Science Research Center, University of South China, 28# Changsheng Western Road, Hengyang 421001, Hunan, China;[Tuo, Qin-Hui; Ke-Tang, Chao; Liao, Duan-Fang; Gong, Yong-Zhen; Zhang, Xiao-Ying; Sun, Shao-Wei] Division of biochemistry, School of pharmacy and Life Science, University of South China, 28# Changsheng West Road, Hengyang 421001, Hunan, China;[Zhang, Xiao-Ying] Division of Pharmacoproteomics, School of pharmacy and Life Science, University of South China, 28# Changsheng Western Road, Hengyang 421001, Hunan, China
Zhang, L;Li, K*;Pardinas, JR;Liao, DF;Li, HJ;Zhang, X
Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology,2004年27(1):75-80 ISSN：1073-6085
[Li, K] Genomapping Inc, Tianjin, Peoples R China.;Jinan Univ, Inst Life Sci & Biotechnol, Guangzhou, Peoples R China.;Chugai Pharma USA, San Diego, CA USA.;Nanhua Univ, Inst Pharm & Pharmacol, Hengyang, Peoples R China.
[Li, K] Genomapping Inc, Tianjin, Peoples R China.
exo plus polymerase;proofreading;SNP;3 ' terminal-labeled primer;phosphorothioate
Single nucleotide polymorphisms (SNPs) are useful physical markers for genetic studies as well as the cause of some genetic diseases. To develop more reliable SNP assays, we examined the underlying molecular mechanisms by which deoxyribonucleic acid (DNA) polymerases with 3′ exonuclease activity maintain the high fidelity of DNA replication. In addition to mismatch removal by proofreading, we have discovered a premature termination of polymerization mediated by a novel OFF-switch mechanism. Two SNP assays were developed, one based on proofreading using 3′ end-labeled primer extension and the other based on the newly identified OFF-switch, respectively. These two new assays are well suited for conventional techniques, such as electrophoresis and microplates detection systems as well as the sophisticated microchips. Application of these reliable SNP assays will greatly facilitate genetic and biomedical studies in the postgenome era.