作者： Ma, Jun;Yan, Ruilan;Zu, Xuyu;Cheng, Ji-Ming;Rao, Krishna;Liao, Duan-Fang;Cao, Deliang

期刊： Journal of Biological Chemistry,2008年283(6):3418-3423 ISSN：0021-9258

通讯作者： Cao, D.

作者机构： [Ma, Jun] Department of Medical Microbiology, Immunology, and Cell Biology, SimmonsCooper Cancer Institute, Southern Illinois University School of Medicine, Springfield, Illinois 62702, USA

通讯机构： [Cao, Deliang] So Illinois Univ, Sch Med, SimmonsCooper Canc Inst, Dept Med Microbiol, 913 N Rutledge St, Springfield, IL 62702 USA.;Dept. of Medical Microbiology, Immunology, and Cell Biology, SimmonsCooper Cancer Inst., Southern Illinois University School of Medicine, 913 N. Rutledge St., United States

关键词： Aldo-keto reductases - Breast cancer cells - Cancer cell growths - Colocalize - Essential components - Fatty acid syntheses - Gel filtrations - Hepatocellular carcinomata - Immunoprecipitation - Ion exchange chromatographies - Lung carcinomata - Mammary epithelial cells - Mass spectrometry analysis - Novel functions - Novel proteins - Proteasome - Protein complexes - Pulldown assays - Sepharose - Small interfering RNAs - Ubiquitination

摘要： Recent studies have demonstrated that aldo-keto reductase family 1 B10 (AKR1B10), a novel protein overexpressed in human hepatocellular carcinoma and non-small cell lung carcinoma, may facilitate cancer cell growth by detoxifying intracellular reactive carbonyls. This study presents a novel function of AKR1B10 in tumorigenic mammary epithelial cells (RAO-3), regulating fatty acid synthesis. In RAO-3 cells, Sephacryl-S 300 gel filtration and DEAE-Sepharose ion exchange chromatography demonstrated that AKR1B10 exists in two distinct forms, monomers (∼40 kDa) bound to DEAE-Sepharose column and protein complexes (∼300 kDa) remaining in flow-through. Co-immunoprecipitation with AKR1B10 antibody and protein mass spectrometry analysis identified that AKR1B10 associates with acetyl-CoA carboxylase-α(ACCA), a rate-limiting enzyme of de novo fatty acid synthesis. This association between AKR1B10 and ACCA proteins was further confirmed by co-immunoprecipitation with ACCA antibody and pulldown assays with recombinant AKR1B10 protein. Intracellular fluorescent studies showed that AKR1B10 and ACCA proteins colocalize in the cytoplasm of RAO-3 cells. More interestingly, small interfering RNA-mediated AKR1B10 knock down increased ACCA degradation through ubiquitination-proteasome pathway and resulted in >50% decrease of fatty acid synthesis in RAO-3 cells. These data suggest that AKR1B10 is a novel regulator of the biosynthesis of fatty acid, an essential component of the cell membrane, in breast cancer cells. ©2008 by The American Society for Biochemistry and Molecular Biology, Inc.

语种： 英文

作者： 谢红艳;胡劲松;殷杰;丁德馨

期刊： 原子能科学技术,2014年48(11):1954-1959 ISSN：1000-6931

通讯作者： Xie, H.-Y.

作者机构： 南华大学药学与生物科学学院,湖南衡阳,421001;[殷杰] 南华大学药学与生物科学学院，湖南衡阳 421001;南华大学铀矿冶生物技术国防重点学科实验室，湖南衡阳 421001;南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳,421001;[殷杰] 南华大学铀矿冶生物技术国防重点学科实验室，湖南衡阳 421001

关键词： 植物组成;超富集植物;铀;植物修复

摘要： 分析研究了某铀尾矿区植物资源，并对这些植物的铀积累作用进行了研究。结果显示，该尾矿区污染土壤上植物群落较简单，共有高等植物31种，隶属12科，其中：禾本科最多，12种；其次是菊科植物，5种；凤尾蕨科和莎草科植物各3种；其他科各1种。1年生或多年生草本植物有28种，占总数的90.3％，其他3种为灌木或小乔木。对这31种植物进行了铀含量测定，结果发现，铀富集量在200 mg／kg以上的植物有14种，占总数的45％，其中富集量在600 mg／kg以上的有3种，分别是水莎草、牧草、小飞蓬。植物体内铀迁移系数大于1的有9种，其中燕麦、牧草、鼠曲草、青蒿中铀的迁移系数较大。这31种植物中，可考虑将水莎草、牧草作为超富集植物应用于铀污染土壤的修复，小飞蓬、盐肤木、枸骨、燕麦、鼠曲草、碎米莎草、龙葵等对铀污染土壤的修复具有潜在应用价值，可进一步研究。

语种： 中文

作者： 杨和平;杨永宗

期刊： 高技术通讯,1997年7(8):49-52 ISSN：1002-0470

作者机构： 衡阳医学院分子生物学研究中心!衡阳;421001;[杨和平; 杨永宗] 衡阳医学院分子生物学研究中心

关键词： 动脉粥样硬化;平滑肌细胞;细胞增殖;细胞凋亡;调节

摘要： 为阐明动脉粥样硬化（AS）斑块发展与稳定的内在机制，围绕动脉增生内膜、AS斑块内平滑肌细胞（SMC）增殖和凋亡密切相关的即刻早期基因（c－fos、c－jun、c－myc）和相关基因（p53、bc1－2、c－sis）表达失衡和重调这一中心展开研究，提出血管SMC增殖和凋亡是决定AS斑块发展的关键环节。

语种： 中文

作者： 刘俊;胡自强

期刊： 湖南师范大学自然科学学报,2006年29(4):89-92+96 ISSN：1000-2537

通讯作者： Liu, J.

作者机构： 湖南师范大学生命科学学院,中国,长沙,410081;南华大学生命科学与技术学院,中国,衡阳,421001;[胡自强] 胡自强;[刘俊] 刘俊

关键词： 软体动物;物种多样性;聚类;湘江衡阳段

摘要： 研究了湘江衡阳江段软体动物的物种多样性.共鉴定软体动物42种,分别隶属于9科20属,其中主要是田螺科和蚌科的种类.10个生境的物种丰富度指数DMA的取值范围在19.914～15.444,Shannon-Wiener多样性指数H’的范围在1.327～0.953,均匀度指数J_(sw)为0.955～0.836.根据不同生境物种的相似性系数C进行系统聚类分析,10个生境在相似性系数0.574 3的水平时全部聚合在一起.

语种： 中文

作者： Wang, Chun;Yan, Ruilan;Luo, Dixian;Watabe, Kounosuke;Liao, Duan-Fang;Cao, Deliang

期刊： Journal of Biological Chemistry,2009年284(39):26742-26748 ISSN：0021-9258

通讯作者： Cao, D.

作者机构： [Wang, Chun] Department of Medical Microbiology, SimmonsCooper Cancer Institute, Southern Illinois University School of Medicine, Springfield, Illinois 62702, USA

通讯机构： [Cao, Deliang] So Illinois Univ, Sch Medicine, Dept Med Microbiol Immunol & Cell Biol, SimmonsCooper Canc Inst, 913 N Rutledge St, Springfield, IL 62702 USA.;Dept. of Medical Microbiology, Immunology, and Cell Biology, Simmons Cooper Cancer Institute, Southern Illinois University School of Medicine, 913 N. Rutledge St., United States

关键词： [carbonyl - Acetyl-CoA carboxylase - Aldo-keto reductase - Apoptosis - Caspase-3 - Cell survival - Cellular lipid - End-products - Epalrestat - Fatty acid synthesis - Human colon - Lipid peroxides - Lipid synthesis - Lung Cancer - Lung carcinoma - Mitochondrial Cytochrome C - Mitochondrial function - Oxidative status - Palmitic acid - Reactive oxygen species - Small intestine - Ubiquitin

摘要： Aldo-keto reductase family 1 member B10 (AKR1B10) is primarily expressed in the normal human colon and small intestine but overexpressed in liver and lung cancer. Our previous studies have shown that AKR1B10 mediates the ubiquitin-dependent degradation of acetyl-CoA carboxylase-α. In this study, we demonstrate that AKR1B10 is critical to cell survival. In human colon carcinoma cells (HCT-8) and lung carcinoma cells (NCIH460), small-interfering RNA-induced AKR1B10 silencing resulted in caspase-3-mediated apoptosis. In these cells, the total and subspecies of cellular lipids, particularly of phospholipids, were decreased by more than 50%, concomitant with 2 - 3-fold increase in reactive oxygen species, mitochondrial cytochrome c efflux, and caspase-3 cleavage. AKR1B10 silencing also increased the levels of α,β-unsaturated carbonyls, leading to the 2 - 3-fold increase of cellular lipid peroxides. Supplementing the HCT-8 cells with palmitic acid (80 μM), the end product of fatty acid synthesis, partially rescued the apoptosis induced by AKR1B10 silencing, whereas exposing the HCT-8 cells to epalrestat, an AKR1B10 inhibitor, led to more than 2-fold elevation of the intracellular lipid peroxides, resulting in apoptosis. These data suggest that AKR1B10 affects cell survival through modulating lipid synthesis, mitochondrial function, and oxidative status, as well as carbonyl levels, being an important cell survival protein. ©2009 by The American Society for Biochemistry and Molecular Biology, Inc.

语种： 英文

作者： Guo, Zifen;Feng, Yong;Huang, Honglin

期刊： Lecture Notes in Electrical Engineering,2014年269(4):3065-3070 ISSN：1876-1100

通讯作者： Guo, Z.(guozifen@aliyun.com)

作者机构： Huang<&wdkj&> H.;[Feng, Yong] Student Affairs Department, University of South China, Hengyang 421001, China;[Huang, Honglin; Guo, Zifen] Department of Pharmacy, University of South China, Hengyang 421001, China

会议名称： 5th International Symposium on IT in Medicine and Education, ITME 2013

会议时间： July 19, 2013 - July 21, 2013

会议地点： Xining, China

会议主办单位： (1) Department of Pharmacy, University of South China, Hengyang 421001, China; (2) Student Affairs Department, University of South China, Hengyang 421001, China

关键词： Education reforms - Educational contents - Flexible Learning - Internet technology - Modern information technologies - Network technologies - Professional knowledge - Teaching philosophy

摘要： With the rapid development and deep penetration of multimedia and internet technology, some tremendous and profound changes have happened in educational content, teaching methods, teaching philosophy and mode, which make it easy for people to access to the knowledge and information of the higher medical education. The perfect combination of multimedia and network technology not only provides a convenient flexible learning space and time for students, but also enables them to master the forefront of medicine and broaden their professional knowledge. The information technology has promoted the modernization process of higher medical education reform and development, so that it will have extremely profound impact on high-quality medical personnel training in China. &copy;Springer Science+Business Media Dordrecht 2014.

语种： 英文

作者： 郭紫芬;陈琳玲;张佳;彭翠英;杨向东;张旭;何淑雅;廖端芳;李凯

期刊： 中华医学遗传学杂志,2003年20(4):328-330 ISSN：1003-9406

通讯作者： Guo, Z.-F.

作者机构： 421001,衡阳,南华大学药物药理研究所;[张佳; 张旭] 津脉公司;421001,衡阳,南华大学生物化学与分子生物学研究所;[郭紫芬; 陈琳玲; 彭翠英; 杨向东; 何淑雅; 廖端芳; 李凯] 南华大学

通讯机构： Dept. of Pharmacy and Pharmacology, Nanhua University, China

关键词： 单核苷酸多态性;高保真聚合酶;硫化修饰引物;DNA聚合反应

摘要： 目的　探讨硫化修饰的次 3′末端不配对引物能否引发高保真 DNA聚合酶介导的聚合反应的非成熟性终止 ,即所谓聚合反应的“关”效应。方法　采用配对及非末端不配对的 3′硫化修饰引物 ,研究其对不同保真度 DNA聚合酶引物延伸反应的影响。结果　非末端不配对的 3′硫化修饰引物也能引发高保真 DNA聚合酶介导的聚合反应非成熟性终止 ,而对低保真 DNA聚合酶所介导的聚合反应则无明显影响。同时 ,3′硫化修饰的配对引物对不同保真度 DNA聚合酶引物延伸反应均无影响。结论　硫化修饰的次 3′末端不配对引物与 3′末端不配对引物对引物延伸的影响相似 ,同样能引起高保真 DNA聚合酶介导的聚合反应的“关”的效应。显然 ,在单基因遗传病的诊断及单核苷酸多态性 (single nucleotide polymorphism,SNP)的高通量分析等方面 ,硫化修饰的碱基特异性引物与高保真 DNA聚合酶所构成的对 SNP敏感的“开 /关”系统 ,具有广阔的应用前景。

语种： 中文

作者： Liu, L.-S.;Wei, D.-H.;Tang, C.-K.;Wang, G.-X.;Zhang, S.-C.;Yin, W.-D.;Yang, Y.-Z.;Legrand, A.-P.;Guidoin, R.

期刊： Artificial Cells, Blood Substitutes, and Biotechnology,2007年35(3):319-331 ISSN：1073-1199

通讯作者： R. Guidoin

作者机构： [Liu, L.-S.] Institute of Cardiovascular Research, South-China University, Hengyang, China

通讯机构： [Guidoin, R.] Univ Laval, Dept Surg, Vandry Bldg, Quebec City, PQ G1K 7P4, Canada.

关键词： Lipofect AMINE;Stable transfection;Vascular tissue engineering;VEGF

摘要： The purpose of this investigation was to establish monoclonal cell lines of HUVEC with the stable expression of the VEGF121 gene. Such cells are likely to better adhere to the luminal surface of stents or grafts and to promote a complete endothelialization. The eukaryotic expression vector PCD2-VEGF121 was transfected into cell lines of HUVEC mediated by lipofect AMINE. The positive clones were obtained by the screening of G418. The transcription and expression of the VEGF gene were investigated by RT-PCR and immunocytochemistry, respectively. The experiment of Miles was applied for the assay of the biological activity of the protein of the VEGF produced by the HUVEC lines with transfected PCD2-VEGF121. The growth curve was made for comparison with that of non-transfected HUVEC line cells. The positive clone cells from which transcripted the mRNA of VEGF121 gene were obtained by RT-PCR. The positive results of the immunocytochemistry were found and the high biological activity of VEGF in the media was detected in the positive clone cells only. The time to achieve the multiplication of the positive clone cells by a factor of 2 was shorter than that of the non-transfected HUVEC line calculated from the growth curve. The HUVEC line of monoclonal cells with the stable expression of VEGF121 gene has been established successfully and can be employed on the luminal surfaces of foreign blood conduits. Copyright &copy;Informa Healthcare.

语种： 英文

作者： Yu, Cui-Yun;Yin, Bio-Cheng;Zhang, Wei;Cheng, Si-Xue;Zhang, Xian-Zheng;Zhuo, Ren-Xi

期刊： Colloids and Surfaces B: Biointerfaces,2009年68(2):245-249 ISSN：0927-7765

通讯作者： Cheng, S.-X.

作者机构： [Yu, Cui-Yun] Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China

通讯机构： [Cheng, Si-Xue] Wuhan Univ, Dept Chem, Minist Educ, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China.;Key Laboratory of Biomedical Polymers, Ministry of Education, Department of Chemistry, China

关键词： Alginate;Chitosan;Controlled drug release;Microparticles;Pectin

摘要： Composite microparticle drug delivery systems based on chitosan, alginate and pectin with improved pH sensitivity were developed for oral delivery of protein drugs, using bovine serum albumin (BSA) as a model drug. The composite drug-loaded microparticles with a mean particle size less than 200 &mu;m were prepared by a convenient shredding method. Since the microparticles were formed by tripolyphosphate cross-linking, electrostatic complexation by alginate and/or pectin, as well as ionotropic gelation with calcium ions, the microparticles exhibited an improved pH-sensitive drug release property. The in vitro drug release behaviors of the microparticles were studied in simulated gastric (pH 1.2 and pH 5.0), intestinal (pH 7.4) and colonic (pH 6.0 and pH 6.8 with enzyme) media. For the composite microparticles with suitable compositions, the releases of BSA at pH 1.2 and pH 5.0 could be effectively sustained, while the releases at pH 7.4, pH 6.8 and pH 6.0 increased significantly, especially in the presence of pectinase. These results clearly suggested that the microparticles had potential for site-specific protein drug delivery through oral administration. &copy;2008 Elsevier B.V. All rights reserved.

语种： 英文

作者： 郭紫芬;陈琳玲;张佳;彭翠英;杨向东;张旭;何淑雅;廖端芳;李凯

期刊： 中华医学遗传学杂志,2003年20(4):328-330 ISSN：1003-9406

通讯作者： Guo, Z.-F.

作者机构： 421001,衡阳,南华大学药物药理研究所;[张佳; 张旭] 津脉公司;421001,衡阳,南华大学生物化学与分子生物学研究所;[郭紫芬; 陈琳玲; 彭翠英; 杨向东; 何淑雅; 廖端芳; 李凯] 南华大学

通讯机构： Dept. of Pharmacy and Pharmacology, Nanhua University, China

关键词： 单核苷酸多态性;高保真聚合酶;硫化修饰引物;DNA聚合反应

摘要： 目的　探讨硫化修饰的次 3′末端不配对引物能否引发高保真 DNA聚合酶介导的聚合反应的非成熟性终止 ,即所谓聚合反应的“关”效应。方法　采用配对及非末端不配对的 3′硫化修饰引物 ,研究其对不同保真度 DNA聚合酶引物延伸反应的影响。结果　非末端不配对的 3′硫化修饰引物也能引发高保真 DNA聚合酶介导的聚合反应非成熟性终止 ,而对低保真 DNA聚合酶所介导的聚合反应则无明显影响。同时 ,3′硫化修饰的配对引物对不同保真度 DNA聚合酶引物延伸反应均无影响。结论　硫化修饰的次 3′末端不配对引物与 3′末端不配对引物对引物延伸的影响相似 ,同样能引起高保真 DNA聚合酶介导的聚合反应的“关”的效应。显然 ,在单基因遗传病的诊断及单核苷酸多态性 (single nucleotide polymorphism,SNP)的高通量分析等方面 ,硫化修饰的碱基特异性引物与高保真 DNA聚合酶所构成的对 SNP敏感的“开 /关”系统 ,具有广阔的应用前景。

语种： 中文

作者： Zhang, L;Li, K;Pardinas, JR;Liao, DF;Li, HJ;Zhang, X

期刊： Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology,2004年27(1):75-80 ISSN：1073-6085

通讯作者： Li, K.

作者机构： Genomapping Inc.<&wdkj&>Jinan University<&wdkj&>Chugai Pharma USA<&wdkj&>Nanhua University;[Jia Zhang; Li, K] Genomapping Inc.;[Pardinas, JR; Zhang, X] Jinan University;[Liao, DF] Chugai Pharma USA;[Li, HJ] Nanhua University

通讯机构： [Li, K] Genomapping Inc, Tianjin, Peoples R China.;Genomapping Inc., China

关键词： Exo+ polymerase;proofreading;SNP;3′ terminal-labeled primer;phosphorothioate

摘要： Single nucleotide polymorphisms (SNPs) are useful physical markers for genetic studies as well as the cause of some genetic diseases. To develop more reliable SNP assays, we examined the underlying molecular mechanisms by which deoxyribonucleic acid (DNA) polymerases with 3′ exonuclease activity maintain the high fidelity of DNA replication. In addition to mismatch removal by proofreading, we have discovered a premature termination of polymerization mediated by a novel OFF-switch mechanism. Two SNP assays were developed, one based on proofreading using 3′ end-labeled primer extension and the other based on the newly identified OFF-switch, respectively. These two new assays are well suited for conventional techniques, such as electrophoresis and microplates detection systems as well as the sophisticated microchips. Application of these reliable SNP assays will greatly facilitate genetic and biomedical studies in the postgenome era.

语种： 英文

作者： Tian, Li;Jia, Lianqun;Fu, Mingde;Tian, Ying;Xu, Yanhua;Tian, Haoming;Yang, Yuye

期刊： Lipids,2006年41(8):789-796 ISSN：0024-4201

通讯作者： Fu, M.

作者机构： Laboratory of Endocrinology and Metabolism, West China HospitalSichuan UniversityChengduSichuan610041People's Republic of China<&wdkj&>Department of BiochemistryLiaoning University of Traditional Chinese MedicineShenyang LiaoningPR China<&wdkj&>Department of Biochemistry and Molecular BiologyNanhua UniversityHengyangHumanPR China<&wdkj&>Hoist Group Postdoctoral Work StationChengduSichuanPR China;[Tian, Li; Fu, Mingde; Tian, Haoming; Yang, Yuye] Sichuan University;[Jia, Lianqun] Liaoning University of Traditional Chinese Medicine;[Tian, Ying] Nanhua University;[Xu, Yanhua] Hoist Group Postdoctoral Work Station

通讯机构： [Fu, Mingde] Sichuan Univ, W China Hosp, Lab Endocrinol & Metab, Chengdu 610041, Sichuan, Peoples R China.

关键词： Obese Subject;Reverse Cholesterol Transport;Overweight Subject;Phospholipid Transfer Protein;Desirable Weight

摘要： The object of this study was to investigate the characteristics of lipid metabolism in obese subjects, with particular emphasis on the alteration of HDL subclass contents and distributions. A population of 581 Chinese individuals was divided into four groups (25 underweight subjects, 288 of desirable weight, 187 overweight, and 45 obese) according to body mass index (BMI). Apoprotein A-I (apoA-I) contents of plasma HDL subclasses were determined by 2-D gel electrophoresis associated with an immunodetection method. The concentrations of TG and the apoA-I content of pre-&beta;<inf>1</inf>-HDL were significantly higher (P &lt;0.01 and P &lt;0.01, respectively), but the levels of HDL cholesterol, and the apoA-I contents of HDL<inf>2a</inf> and HDL<inf>2b</inf> were significantly lower (P &lt;0.01, P &lt;0.05, and P &lt;0.01, respectively) in obese subjects than in subjects having a desirable weight. Moreover, with the elevation of BMI, small-sized pre-&beta;<inf>1</inf>-HDL increased gradually and significantly, whereas large-sized HDL<inf>2b</inf> decreased gradually and significantly. Meanwhile, the variations in HDL subclass distribution were more obvious with the elevation of TG levels in obese as well as overweight subjects. In addition, Pearson correlation analysis revealed that BMI and TG levels were positively correlated with pre-&beta;<inf>1</inf>-HDL but negatively correlated with HDL<inf>2b</inf>. Multiple regression analysis also showed that TG concentrations were associated independently and positively with high pre-&beta;<inf>1</inf>-HDL and independently and negatively with low HDL<inf>2b</inf> in obese and overweight subjects. The HDL particle size was smaller in obese and overweight subjects. The shift to smaller size was more obvious with the elevation of BMI and TG, especially TG levels. These observations, in turn, indicated that HDL maturation might be abnormal, and reverse cholesterol transport might be impaired. Copyright &copy;2006 by AOCS Press.

语种： 英文