作者机构：
[Xiao, L.; Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215006, Peoples R China.;[Xiao, L.; Li, Kai] Suzhou Univ, Second Affilated Hosp, Suzhou 215006, Peoples R China.;[Xiao, L.; Zhang, J.; Chen, C. L.; Li, Kai] Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.;[Zhang, J.] Novartis Res Fdn, Genom Inst, San Diego, CA USA.;[Yin, Y. F.] City Hope Natl Med Ctr, Duarte, CA 91010 USA.
通讯机构：
[Zhang, I ] ;Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.
摘要：
A new method was developed to fabricate nanospheres and vesicles as drug carriers. The drug-loaded nanospheres and vesicles were prepared by self-assembly of alginate in aqueous media containing Ca2+ and CO32− ions under very mild conditions. The preparation method did not involve any organic solvent and surfactant and could offer good control over the morphology and the size of self-assemblies. Through adjusting the preparation conditions, nanosized drug-delivery systems with different shapes, that is, nanospheres and vesicles, could be obtained. The morphologies of the drug-delivery systems were observed by transmission electron microscopy (TEM). 5-Fluorouracil (5-FU), an anticancer drug, was encapsulated in the nanospheres and vesicles, and in vitro drug release behavior was investigated. The effect of drug-loading content on the release was studied. The release of 5-FU could be effectively sustained from both drug-loaded nanospheres and vesicles because the presence of CaCO3 in the nanospheres/vesicles could reduce the permeability of the entrapped drug for the alginate-based self-assemblies.
摘要：
Alterations in plasma apolipoproteins levels can influence the composition, content, and distribution of plasma lipoproteins that affect the risk of atherosclerosis. This study assessed the relationship between plasma apolipoproteins levels, mainly apoAI, and HDL subclass distribution. The contents of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection in 545 Chinese subjects. Compared with a low apoAI group, the contents of all HDL subclasses increased significantly both in middle and high apoAI group, and the contents of large-sized HDL(2b) increased more significantly relative to those of small-sized prebeta(1)-HDL in a high apoAI group. When apoAI and HDL-C levels increased simultaneously, in comparison to a low apoAI along with HDL-C concentration group, a significant increase (116%) was shown in HDL2b but only a slight increase (26%) in prebeta1-HDL. In addition, Pearson correlation analysis revealed that apoAI levels were positively and significantly correlated with all HDL subclasses. Multiple liner regression demonstrated that the apoAI concentrations were the most powerful predictor for HDL subclass distribution. With the elevation of apoAI concentrations, the contents of all HDL subclasses increased successively and significantly, especially, an increase in large-sized HDL(2b). Further, when apoAI and HDL-C concentrations increased simultaneously, the shift to larger HDL size was more obvious. Which, in turn, indicated that HDL maturation might be enhanced and, the reverse cholesterol transport might be strengthened along with apoAI levels which might be a more powerful factor influencing the distribution of HDL subclasses.
作者机构：
[胡自强; 刘俊] College of Life Science, Hunan Normal University, Changsha 410081, China;[刘俊] College of Life Science and Technology, Nanhua University, Hengyang 421001, China
摘要：
The object of this study was to investigate the characteristics of lipid metabolism in obese subjects, with particular emphasis on the alteration of HDL subclass contents and distributions. A population of 581 Chinese individuals was divided into four groups (25 underweight subjects, 288 of desirable weight, 187 overweight, and 45 obese) according to body mass index (BMI). Apoprotein A-1 (apoA-1) contents of plasma HDL subclasses were determined by 2-D gel electrophoresis associated with an immunodetection method. The concentrations of TG and the apoA-I content of pre-beta(1)-HDL were significantly higher (P < 0.01 and P < 0.01, respectively), but the levels of HDL cholesterol, and the apoA-1 contents of HDL2a and HDL2b were significantly lower (P < 0.01, P < 0.05, and P < 0.01, respectively) in obese subjects than in subjects having a desirable weight. Moreover, with the elevation of BMI, small-sized pre-beta 1-HDL increased gradually and significantly, whereas large-sized HDL2b decreased gradually and significantly. Meanwhile, the variations in HDL subclass distribution were more obvious with the elevation of TG levels in obese as well as overweight subjects. In addition, Pearson correlation analysis revealed that BMI and TG levels were positively correlated with pre-beta(1)-HDL but negatively correlated with HDL2b. Multiple regression analysis also showed that TG concentrations were associated independently and positively with high pre-beta(1)-HDL and independently and negatively with low HDL2b in obese and overweight subjects. The HDL particle size was smaller in obese and overweight subjects. The shift to smaller size was more obvious with the elevation of BMI and TG, especially TG levels. These observations, in turn, indicated that HDL maturation might be abnormal, and reverse cholesterol transport might be impaired.
期刊：
Trends in Biotechnology,2005年23(2):92-96 ISSN：0167-7799
通讯作者：
Li, K
作者机构：
[Li, K] Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.;Geomapping Inc, Tianjin, Peoples R China.;City Hope Natl Med Ctr, Dept Human Genet, Duarte, CA 91010 USA.;First Mil Med Univ, Inst Oncol, Guangzhou 510515, Peoples R China.
通讯机构：
[Li, K] N;Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.
摘要：
DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3' dehydroxylation modifications. Accordingly, three new types of single nucleotide polymorphism (SNP) assays have been developed to carry out genome-wide genotyping making use of the fidelity advantage of exo(+) polymerases. Such SNP assays might also provide a novel approach for re-sequencing and de novo sequencing. These new mutation detection assays are widely adaptable to a variety of platforms, including real-time PCR, multi-well plate and microarray technologies. Application of exo(+) polymerases to genetic analysis could accelerate the pace of personalized medicine.
作者机构：
[蒋小云; 刘云海; 熊熙文; 张健; 胡小晓] Lab. Gene Knock-out/Transgenic Anim., College of Life Science, Hunan Normal University, Changsha 410081, China;[刘鑫] Dept. of Laboratory Animal Science, Coll. of Life Science and Technology, Nanhua University, Hengyang 421001, China
作者机构：
[Li, K] Genomapping Inc, Tianjin, Peoples R China.;Jinan Univ, Inst Life Sci & Biotechnol, Guangzhou, Peoples R China.;Chugai Pharma USA, San Diego, CA USA.;Nanhua Univ, Inst Pharm & Pharmacol, Hengyang, Peoples R China.
通讯机构：
[Li, K] G;Genomapping Inc, Tianjin, Peoples R China.
关键词：
exo plus polymerase;proofreading;SNP;3 ' terminal-labeled primer;phosphorothioate
