期刊论文

Li, K

英文

Trends in Biotechnology

0167-7799

2005

23

2

92-96

10.1016/j.tibtech.2004.12.009

This study was supported by the Chinese national major basic research program (973: G2000056905) and Hunan natural science foundation (03JJY4041). We thank Drs Saldivar, Gao and Liu, Ms Lesiecki, Mr Scaringe and Mr Mroske for proofreading of this manuscript. Some of the polymerases used in our study were a free gift from NEB.

本校为第一且通讯机构

DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3' dehydroxylation modifications. Accordingly, three new types of single nucleotide polymorphism (SNP) assays have been developed to carry out genome-wide genotyping making use of the fidelity adva...