版权说明 操作指南
首页 > 成果 > 详情

Proofreading genotyping assays mediated by high fidelity exo+ DNA polymerases

认领
导出
Link by DOI
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
Zhang, J;Li, K*;Pardinas, JR;Sommer, SS;Yao, KT
通讯作者:
Li, K
作者机构:
[Li, K] Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.
Geomapping Inc, Tianjin, Peoples R China.
City Hope Natl Med Ctr, Dept Human Genet, Duarte, CA 91010 USA.
First Mil Med Univ, Inst Oncol, Guangzhou 510515, Peoples R China.
通讯机构:
[Li, K] Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
期刊:
Trends in Biotechnology
ISSN:
0167-7799
年:
2005
卷:
23
期:
2
页码:
92-96
机构署名:
本校为第一且通讯机构
院系归属:
药学与生物科学学院
摘要:
DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3' dehydroxylation modifications. Accordingly, three new types of single nucleotide polymorphism (SNP) assays have been developed to carry out genome-wide genotyping making use of the fidelity advantage of exo(+) polymerases. Such SNP assays might also provide a novel approach for re-sequencing and de novo sequencing. These new mutation detection assays are widely adaptable to a variety of platforms, including real-time PCR, multi-well plate and microarray technologies. Application of exo(+) polymerases to genetic analysis could accelerate the pace of personalized medicine.

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com