期刊论文

Guidoin, R.

英文

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY

2169-1401

2007

35

3

319-331

10.1080/10731190701378642

Guinea pigs were injected by Evans blue through sublingual vein. After 30 minutes, 1,1/2 and 1/4 original culture medium of stably transfected cells were injected by hypodermic injections [33]. The injected dot diameter was about 5 mm. After 15 minutes, the permeability was detected by the subcutaneous blue dot emergence or not and their diameter were quantified. This protocol was supported by the Committee on Experimental Animal Care of the University of Nanhua (Province of Hunan, PR China).

本校为其他机构

The purpose of this investigation was to establish monoclonal cell lines of HUVEC with the stable expression of the VEGF121 gene. Such cells are likely to better adhere to the luminal surface of stents or grafts and to promote a complete endothelialization. The eukaryotic expression vector PCD2-VEGF121 was transfected into cell lines of HUVEC mediated by lipofect AMINE. The positive clones were obtained by the screening of G418. The transcription and expression of the VEGF gene were investigated by RT-PCR and immunocytochemistry, respectively. The experiment of Miles was applied for the assay ...