作者机构：
[Li, Yu; Jiang, Zhong-Xing; Zhu, Junfei; Zhang, Huaibin; Yang, Zhigang] Wuhan Univ, Sch Pharmaceut Sci, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Wuhan 430071, Peoples R China.;[Xiao, Yan; Zheng, Xing] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Zhou, Xin; Chen, Shizhen; Yuan, Yaping] Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan, Wuhan 430071, Peoples R China.
通讯机构：
Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
摘要：
Thermosensitive and imaging-traceable materials with fine-tunable lower critical solution temperature (LCST) around body temperature are highly valuable in biomedicine. However, such materials are rare because it is challenging to fine-tune the LCST and incorporate suitable imaging modalities. Herein, peptidic monodisperse polyethylene glycol (M-PEG) "combs" with fine-tunable LCST, "hot spot" fluorine-19 magnetic resonance imaging ((19)F MRI), thermoresponsive fluorescent imaging, and drug loading ability were developed through accurately programming their structures during solid phase peptide synthesis (SPPS). The easy availability, structural accuracy, biocompatibility, and versatility provide the M-PEG "combs" with promising prospects as thermoresponsive and imaging-traceable biomaterials for controlled drug delivery.
摘要：
The objective of this study was to apply the "on/off" switch consisting of 3' phosphorothioate-modified allele specific primers and exo(+) polymerase in single base discrimination of A1555G and C1494T mutations in the highly conserved sites of the mitochondrial 12S rRNA. The two point mutations are the hotspot mutations associated with either aminoglycoside antibiotics induced deafness or inherited nonsyndromic hearing loss. The PCR products of mitochondrial DNA (mtDNA) 12S rRNA gene were inserted into the pMD19-T vector for transformation into Escherichia coli JM109 competent cells for preparing wild-type pMD19-T/mt vector. Inverse PCR was carried out for mtDNA 12S rRNA gene C1494T and A1555G mutagenesis and DpnI endonuclease degradating methylated pMD19-T/mt vector existing in the inverse PCR products was carried out to construct the mutation-type pMD19-T/mtM vector. These constructed vectors were confirmed by DNA sequencing. Allelic specific primers targeting wild-type and mutation-type templates were designed with 3' terminal phosphorothioate modification. Two-directional primer extension was performed using Pfu polymerases. Amplified by exo(+) polymerase, allelic specific primers perfectly matching wild-type allele were extended while no products were produced from primers targeting point-mutated deafness-related allele. Similarly, allelic specific primers perfectly matching point-mutated deafness-related mutation-type allele were extended and no products were yielded from primers targeting wild-type allele. No specific product was observed in the primer extension reaction mediated by on/off switch in screening the mtDNA 12S rRNA gene harboring either C1494T or A1555G mutation in 40 healthy volunteers tested. These data suggest that the "off switch" mediated by exo(+) polymerase is highly reliable in the diagnosis of monogenic diseases and the novel "on/off" switch has enormous applications in systematic and extended screening of the12S rRNA gene A1555G and C1494T mutations. The established assay can be widely used not only for hearing loss patients but also for normal subjects before the use of aminoglycoside antibiotics.
作者机构：
[Wang, Zong-Bao; Wei, Yun; Xiao, Ji-Chang; Zheng, Qu-Tong; Zheng, Xing] Univ South China, Inst Pharm & Pharmacol, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Wang, Zong-Bao; Wei, Yun; Xiao, Ji-Chang; Zheng, Qu-Tong; Zheng, Xing] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, 28 Western Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Duan, Ya-ya; Wei, Yun; Lin, Jin-Hong; Xiao, Ji-Chang; Zheng, Jian; Zheng, Qu-Tong; Zhao, Gang] Univ Chinese Acad Sci, Chinese Acad Sci, Key Lab Organofluorine Chem, Shanghai Inst Organ Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China.
通讯机构：
Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, China
通讯机构：
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, Hunan, China
期刊：
Organic and Biomolecular Chemistry,2018年16(44):8537-8545 ISSN：1477-0520
通讯作者：
Jiang, Zhong-Xing(zxjiang@whu.edu.cn)
作者机构：
[Jiang, Zhong-Xing; Lv, Xiaoyan; Yang, Zhigang] Wuhan Univ, Sch Pharmaceut Sci, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Wuhan 430071, Hubei, Peoples R China.;[Zheng, Xing] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
通讯机构：
[Lv, Xiaoyan] Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
摘要：
Dual-functionalization of monodisperse oligoethylene glycols, especially hetero-functionalization, provides a series of highly valuable intermediates for life and materials sciences. However, the existing methods for the preparation of these compounds suffer excessive protecting and activating group manipulation as well as tedious purification. Here, a one-pot dual-substitution strategy with macrocyclic sulfates of polyethylene glycols as the key intermediates was developed for the convenient and scalable preparation of a series of homo-functionalized and hetero-functionalized oligoethylene glycols in just 1 step. A high synthetic efficacy was achieved by avoiding the protecting and activating group manipulation and the intermediate purification.
期刊：
Trends in Biotechnology,2005年23(2):92-96 ISSN：0167-7799
通讯作者：
Li, K
作者机构：
[Li, K] Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.;Geomapping Inc, Tianjin, Peoples R China.;City Hope Natl Med Ctr, Dept Human Genet, Duarte, CA 91010 USA.;First Mil Med Univ, Inst Oncol, Guangzhou 510515, Peoples R China.
通讯机构：
[Li, K] Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.
摘要：
DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3' dehydroxylation modifications. Accordingly, three new types of single nucleotide polymorphism (SNP) assays have been developed to carry out genome-wide genotyping making use of the fidelity advantage of exo(+) polymerases. Such SNP assays might also provide a novel approach for re-sequencing and de novo sequencing. These new mutation detection assays are widely adaptable to a variety of platforms, including real-time PCR, multi-well plate and microarray technologies. Application of exo(+) polymerases to genetic analysis could accelerate the pace of personalized medicine.
作者机构：
[肖方竹; 何淑雅; 唐艳] Support Discipline Laboratory of National Defence for Biochemistry and Molecular biology, University of South China, Hengyang, 421001, China;[戴益民] The Key Laboratory of Powder and Transport Materials Protection in Hunan Province, College of Chemistry and Chemical Engineering, Changsha University of Science &, Technology, Changsha, 410004, China;[彭国文] School of Resources and Safety Engineering, Central South University, Changsha, 410083, China;[彭国文] School of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, China
通讯机构：
School of Chemistry and Chemical Engineering, University of South China, Hengyang, China
作者机构：
[Xiao, L.; Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215006, Peoples R China.;[Xiao, L.; Li, Kai] Suzhou Univ, Second Affilated Hosp, Suzhou 215006, Peoples R China.;[Xiao, L.; Zhang, J.; Chen, C. L.; Li, Kai] Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.;[Zhang, J.] Novartis Res Fdn, Genom Inst, San Diego, CA USA.;[Yin, Y. F.] City Hope Natl Med Ctr, Duarte, CA 91010 USA.
通讯机构：
[Zhang, I ] Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.;Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.
摘要：
We propose a short definition of GENOME: The full complement of genetic materials possessed by an intracellular parasite, a cell, or an organism. Accordingly, the human genome is the entire complement of inherited genetic materials possessed by an individual person, or possessed by a cell in an individual person. For higher species, the genomic makeup includes DNA in the nucleus and in the organelles regardless of the number of chromosomes and the homoplasmic or heteroplasmic status of the mitochondrial or chloroplastic DNA. Practically, GENOME can be referred to at the molecular, cellular, individual, and species levels, which has various implications in biotechnological research and molecular diagnostics.
摘要：
A new method was developed to fabricate nanospheres and vesicles as drug carriers. The drug-loaded nanospheres and vesicles were prepared by self-assembly of alginate in aqueous media containing Ca2+ and CO32− ions under very mild conditions. The preparation method did not involve any organic solvent and surfactant and could offer good control over the morphology and the size of self-assemblies. Through adjusting the preparation conditions, nanosized drug-delivery systems with different shapes, that is, nanospheres and vesicles, could be obtained. The morphologies of the drug-delivery systems were observed by transmission electron microscopy (TEM). 5-Fluorouracil (5-FU), an anticancer drug, was encapsulated in the nanospheres and vesicles, and in vitro drug release behavior was investigated. The effect of drug-loading content on the release was studied. The release of 5-FU could be effectively sustained from both drug-loaded nanospheres and vesicles because the presence of CaCO3 in the nanospheres/vesicles could reduce the permeability of the entrapped drug for the alginate-based self-assemblies.
作者机构：
[李俐娟; 何淑雅; 苏泽红] Department of Biotechnology, School of Pharmacology and Life Sciences and Technology, University of South China, Hengyang 421001, China;[练高建] Department of Experimental Zoology, University of South China, Hengyang 421001, China;[Takahashi, Akira] Department of Preventive and Environment Nutrition, Tokushima University, Tokushima, 770-8501, Japan
通讯机构：
Department of Experimental Zoology, University of South China, China
作者：
Zhang, Jia;Xiao, Li;Yin, Yufang;Sirois, Pierre;Gao, Hanlin;Li, Kai
期刊：
Applied Biochemistry and Biotechnology,2010年162(2):321-328 ISSN：0273-2289
通讯作者：
Li, K.(kaili34@yahoo.com)
作者机构：
[Xiao, Li; Zhang, Jia] Soochow Univ, Affiliated Hosp 2, Clin Mol Diagnost Ctr, Suzhou 215004, Peoples R China.;[Yin, Yufang] Univ S China, SNP Inst, Henyang 421001, Peoples R China.;[Sirois, Pierre] Univ Sherbrooke, Dept Pharmacol, Sherbrooke, PQ J1H 5N4, Canada.;[Gao, Hanlin] City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA.;[Li, Kai] Soochow Univ, Coll Med, Dept Pharmacol, Suzhou 215123, Peoples R China.
通讯机构：
Department of Pharmacology, Medical College, Soochow University, China
关键词：
Cancer;Indels;Length distribution
摘要：
Indels in evolutionary studies are rapidly decayed obeying a power law. The present study analyzed the length distribution of small insertions and deletions associated with human diseases and confirmed that the decay pattern of these small mutations is similar to that of indels when the mutation datasets are large enough. The describable decay pattern of somatic mutations may have application in the evaluation of varied penetrance of different mutations and in association study of gene mutation with carcinogenesis.
作者机构：
[蒋小云; 刘云海; 熊熙文; 张健; 胡小晓] Lab. Gene Knock-out/Transgenic Anim., College of Life Science, Hunan Normal University, Changsha 410081, China;[刘鑫] Dept. of Laboratory Animal Science, Coll. of Life Science and Technology, Nanhua University, Hengyang 421001, China
会议名称：
2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
会议时间：
October 17, 2009 - October 19, 2009
会议地点：
Tianjin, China
会议主办单位：
(1) Institute of Clinical Medicine, University of South China, Hengyang, 421001, Hunan Province, China; (2) Institute of Pharmacology and Pharmacy, University of South China, Western Changsheng Road 28, Hengyang 421001, Hunan Province, China
会议论文集名称：
International Conference on Biomedical Engineering and Informatics
作者机构：
[殷杰; 谢红艳; 胡劲松] School of Pharmacy and Biological Sciences, University of South China, Hengyang, China;[殷杰; 丁德馨] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
通讯机构：
School of Pharmacy and Biological Sciences, University of South China, Hengyang, China
