Localization and characterization of a putative cysteine desulfurase in Chlamydia psittaci.
作者：
Wen, Yating;Chen, Yanbo;Li, Li;Xu, Man;Tan, Yuan;...
期刊：
JOURNAL OF CELLULAR BIOCHEMISTRY ,2019年120(3):4409-4422 ISSN：0730-2312
通讯作者：
Wu, Yimou
作者机构：
[Wu, Yimou; Li, Yumeng; Kuang, Xingxing; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pathogen Biol,Med Coll, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang, Peoples R China.;[Chen, Yanbo] Jiangmen Wuyi Tradit Chinese Med Hosp, Dept Clin Lab, Jiangmen, Peoples R China.;[Li, Li] Hunan Prov Ctr Dis Control & Prevent, Toxicol Lab, Changsha, Hunan, Peoples R China.;[Wu, Yimou] Univ South China, Med Coll, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Med Coll, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
cellular localization;Chlamydia psittaci;cysteine desulfurase;mitochondria-mediated pathway;T3SS
摘要：
Chlamydia psittaci is an obligate intracellular pathogen with a biphasic developmental life cycle. It is auxotrophic for a variety of essential metabolites and obtains amino acids from eukaryotic host cells. Chlamydia can develop inside host cells within chlamydial inclusions. A pathway secreting proteins from inclusions into the host cellular cytoplasm is the type III secretion system (T3SS). The T3SS is universal among several Gram-negative bacteria. Here, we show that CPSIT_0959 of C. psittaci is expressed midcycle and secreted into the infected cellular cytoplasm via the T3SS. Recombinant CPSIT_0959 possesses cysteine desulfurase and PLP-binding activity, which removes sulfur from cysteine to produce alanine, and helps chlamydial replication. Our study shows that CPSIT_0959 improve the infectivity of offspring elementary bodies and seems to promote the replication by its product. This phenomenon has inhibited by the PLP-dependent enzymes inhibitor. Moreover, CPSIT_0959 increased expression of Bim and tBid, and decreased the mitochondrial membrane potential of host mitochondria to induce apoptosis in the latecycle for release of offspring. These results demonstrate that CPSIT_0959 has cysteine desulfurase and PLP-binding activity and is likely to contribute to apoptosis of the infected cells via a mitochondria-mediated pathway to improve the infectivity of progeny.
语种：
英文
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基于HPS的独立学院临床技能培养模式的研究
作者：
罗祎敏;李熠
期刊：
现代经济信息 ,2019年(22):398 ISSN：1001-828X
作者机构：
南华大学医学院;[李熠; 罗祎敏] 南华大学
关键词：
临床技能培养
摘要：
高级综合模拟人（HPS）,可使"真实临床场景"再现。HPS用于独立学院临床技能培养,可以真正的大大缩短从临床理论到临床操作的过程。以适应新形势对大学生人才培养的需要,是高等学校教育改革的重要内容。
语种：
中文
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S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway
作者：
Zheng, Zhi;Zeng, Yong-Zhi;Ren, Kun;Zhu, Xiao;Tan, Ying;...
期刊：
International Immunopharmacology ,2019年66(3):224-235 ISSN：1567-5769
通讯作者：
Yi, Guang-Hui
作者机构：
[Li, Qian; Ren, Kun; Zheng, Zhi; Zeng, Yong-Zhi; Tan, Ying; Yi, Guang-Hui; Li, Yi; Zhu, Xiao] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yi, Guang-Hui] U;Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Inflammation;Interleukin-1beta;Lymphangiogenesis;Sphingosine-1-phosphate;Tumor necrosis factor-alpha
摘要：
Inflammation-induced lymphangiogenesis is a widely accepted concept. However, most of the inflammatory factors and their related mechanisms have not been clarified. It has been reported that sphingosine-1-phosphate (S1P) is not only closely related to the chronic inflammatory process but also affects angiogenesis. Therefore, we investigated the inflammatory effects of S1P on human lymphatic endothelial cells (HLECs). Our results showed that S1P promotes tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) secretion in HLECs. We also confirmed that S1P-stimulated TNF-α and IL-1β secretion is mediated through S1P receptor 1 (S1PR1). Using TNF-α siRNA and IL-1β siRNA, we found that TNF-α and IL-1β play essential roles in S1P-induced HLEC proliferation, migration, and tube formation. S1P induces phosphorylation of NF-κB p65 and activation of NF-κB nuclear translocation. A S1PR1 antagonist (W146) and NF-κB inhibitor (BAY11-7082) inhibited S1P-induced TNF-α and IL-1β secretion and prevented NF-κB nuclear translocation. Taken together, the results demonstrated for the first time that S1P promotes the secretion of TNF-α and IL-1β in HLECs via S1PR1-mediated NF-κB signaling pathways, thus affecting lymphangiogenesis. The study provides a new strategy for finding treatments for lymphangiogenesis-related diseases. © 2018 Elsevier B.V.
语种：
英文
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Involvement of TRPV1 in the expression and release of calcitonin gene-related peptide induced by rutaecarpine
作者：
Yang, Yongmei;Chen, Qingquan;Jia, Sujie;He, Limei;Wang, Aiping;...
期刊：
MOLECULAR MEDICINE REPORTS ,2018年17(4):5168-5174 ISSN：1791-2997
通讯作者：
Li, Xiaohui
作者机构：
[He, Limei; Chen, Qingquan; Li, Xiaohui; Yang, Yongmei; Li, Yuanjian] Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, 110 Xiang Ya Rd, Changsha 410078, Hunan, Peoples R China.;[Wang, Aiping; Yang, Yongmei] Univ South China, Sch Med, Dept Anat, Hengyang 421001, Hunan, Peoples R China.;[Jia, Sujie] Cent S Univ, Third Xiangya Hosp, Dept Pharm, Changsha 410013, Hunan, Peoples R China.;[Li, Dai] Cent S Univ, Natl Inst Drug Clin Trial, Xiangya Hosp, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Li, Xiaohui] C;Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, 110 Xiang Ya Rd, Changsha 410078, Hunan, Peoples R China.
关键词：
calcitonin gene-related peptide;hypertension;rutaecarpine;transient receptor potential cation channel subfamily V member 1
摘要：
The traditional Chinese herb Wu-Chu-Yu has been used to treat hypertension for hundreds of years. A previous study indicated that rutaecarpine was the effective component of Wu-Chu-Yu, which lowered blood pressure by elevating the expression level of calcitonin gene-related peptide (CGRP). The present-study was performed to investigate the role of transient receptor potential cation channel subfamily V member 1 (TRPV1) in CGRP expression and release induced by rutaecarpine. Dorsal root ganglia (DRG) obtained from Sprague-Dawley rats were cultured to analyze the mRNA expression and release of CGRP. Calcium influx, as an indicator of TRPV1 activation, was measured in 293 cells with stable overexpression of TRPV1. The results demonstrated that the amount of CGRP in the cell culture supernatant and the mRNA expression of CGRPα and CGRPβ in DRG was upregulated by rutaecarpine in a concentration-dependent manner, and was inhibited by the TRPV1 receptor antagonist capsazepine. In addition, intracellular Ca 2+ levels were increased by Rut in the aforementioned 293 cell line, indicating the activation of TRPV1 by Rut. Therefore, it was concluded that TRPV1 was involved in the expression and release of CGRP stimulated by rutaecarpine, which provided novel mechanistic understanding of the treatment of hypertension using the Chinese herb Wu-Chu-Yu. © 2018 Kashyap.
语种：
英文
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人工智能在医学生临床技能培养中的应用探讨
作者：
李熠;匡双玉;桂庆军;尹凯;钟慧;...
期刊：
医学教育研究与实践 ,2018年26(6):908-910+992 ISSN：2096-3181
作者机构：
南华大学 医学院临床技能教学中心 ,湖南 衡阳,421001;南华大学附属第二医院临床药学科 ,湖南 衡阳,421001;[匡双玉] 南华大学第二附属医院;[李熠; 钟慧; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
人工智能;临床技能;应用
摘要：
随着技术的不断发展,人工智能在医学诊疗方面的运用越来越广泛,文中阐述了目前人工智能发展的技术水平和特点,对人工智能在医学生临床技能教学中的应用进行了初步探讨,并对人工智能技术在医学教育中的发展前景作了展望。
语种：
中文
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虚拟仿真教学平台在诊断学实验教学中的探索与思考
作者：
钟慧;李熠;游咏;谢娟;陈雯;...
期刊：
教育现代化 ,2018年5(44):189-190 ISSN：2095-8420
作者机构：
南华大学 医学院,湖南 衡阳;[李熠; 钟慧; 谢娟; 冯聚玲; 尹凯; 陈雯; 游咏; 许丽芳] 南华大学
关键词：
虚拟仿真;诊断学实验教学;探索
摘要：
随着社会的进步,传统的医学教育模式难以满足需要,迫切需要改革。以计算机多媒体技术为基础的虚拟仿真教学平台已发展成为新生的医学教育模式,它与诊断学实验教学的结合能提高教学效率和质量,是诊断学教学改革的重要举措之一。
语种：
中文
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Pharmacological inhibition of MALT1 protease activity suppresses endothelial activation via enhancing MCPIP1 expression
作者：
Li, Yong;Huang, Shengping;Huang, Xuan;Li, Xiuzhen;Falcon, Adrian;...
期刊：
Cellular Signalling ,2018年50:1-8 ISSN：0898-6568
通讯作者：
Fu, Mingui
作者机构：
[Li, Xiuzhen; Huang, Shengping; Falcon, Adrian; Soutar, Adele; Li, Yong; Fu, Mingui; Huang, Xuan] Univ Missouri, Sch Med, Dept Biomed Sci, 2411 Holmes St, Kansas City, MO 64108 USA.;[Li, Xiuzhen; Huang, Shengping; Falcon, Adrian; Soutar, Adele; Li, Yong; Fu, Mingui; Huang, Xuan] Univ Missouri, Sch Med, Shock Trauma Res Ctr, 2411 Holmes St, Kansas City, MO 64108 USA.;[Xin, Hong-Bo; Li, Yong; Huang, Xuan] Nanchang Univ, Inst Translat Med, 999 Xuefu Rd, Nanchang 330031, Jiangxi, Peoples R China.;[Bornancin, Frederic] Novartis Inst BioMed Res, Novartis Campus, Basel, Switzerland.;[Jiang, Zhisheng] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Fu, Mingui] U;Univ Missouri, Sch Med, Dept Biomed Sci, 2411 Holmes St, Kansas City, MO 64108 USA.;Univ Missouri, Sch Med, Shock Trauma Res Ctr, 2411 Holmes St, Kansas City, MO 64108 USA.
关键词：
*Endothelial activation;*Inhibitors of MALT1;*MCPIP1, vascular inflammation;*VCAM-1
摘要：
Mucosa associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is not only an intracellular signaling scaffold protein but also a paracaspase that plays a key role in the signal transduction and cellular activation of lymphocytes and macrophages. However, its role in endothelial cells remains unknown. Here we report that pharmacological inhibition of MALT1 protease activity strongly suppresses endothelial activation via enhancing MCPIP1 expression. Treatment with MALT1 protease inhibitors selectively inhibited TNFα-induced VCAM-1 expression in HUVECs and LPS-induced VCAM-1 expression in mice. In addition, Inhibition of MALT1 protease activity also significantly inhibited TNFα-induced adhesion of THP-1 monocytic cells to HUVECs. To explore the mechanisms, MALT1 inhibitors does not affect the activation of NF-κB signaling pathway in HUVEC. However, they can stabilize MCPIP1 protein and significantly enhance MCPIP1 protein level in endothelial cells. These results suggest that MALT1 paracaspase also targets MCPIP1 and degrade MCPIP1 protein in endothelial cells similar as it does in immune cells. Taken together, the study suggest inhibition of MALT1 protease activity may represent a new strategy for prevention/therapy of vascular inflammatory diseases such as atherosclerosis.
语种：
英文
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Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice
作者：
Tan, Yuan;Li, Yumeng;Zhang, Yang;Yu, Jian;Wen, Yating;...
期刊：
Immunologic Research ,2018年66(4):471-479 ISSN：0257-277X
通讯作者：
Wu, Yimou
作者机构：
[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.;[Tan, Yuan] First Hosp Changsha City, Dermatol, Changsha 410000, Hunan, Peoples R China.;[Zhang, Yang] Univ South China, Dept Pathol, Hengyang 421001, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.
关键词：
*C. psittaci;*CD4+ T cells;*CPSIT_p7;*Plasmid protein;*Protective immunity
摘要：
The present study evaluated the immune-protective efficacy of the Chlamydia psittaci (C. psittaci) plasmid protein CPSIT_p7 and analyzed the potential mechanisms of this protection. The current study used recombinant CPSIT_p7 protein with Freund’s complete adjuvant and Freund’s incomplete adjuvant to vaccinate BALB/c mice. Adjuvants alone or PBS formulated with the same adjuvants was used as negative controls. Mice were intranasally challenged with 105 inclusion-forming units (IFU) of C. psittaci. We found that CPSIT_p7 vaccination significantly decreased the mouse lung chlamydial load, interferon-γ (IFN-γ) level, and pathological injury. This protection correlated well with specific humoral and cellular immune responses against C. psittaci. In vitro or in vivo neutralization of C. psittaci with sera harvested from immunized mice did not reduce the number of recoverable C. psittaci in the infected lungs, but CD4+ spleen cells collected from CPSIT_p7-immunized mice significantly decreased the chlamydial load via adoptive transfer to native mice. These results reveal that the protection conferred by CPSIT_p7 is dependent on CD4+ T cells. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
语种：
英文
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基于自主学习能力培养要求的临床技能学教学资源的优化改革与实践
作者：
李熠;匡双玉;游咏;谢娟;钟慧;...
期刊：
教育现代化 ,2018年5(39):56-58,61 ISSN：2095-8420
作者机构：
南华大学医学院诊断学教研室,湖南 衡阳;南华大学附属第二医院,湖南 衡阳;南华大学医学院人文教研室,湖南衡阳;[匡双玉] 南华大学第二附属医院;[李熠; 钟慧; 谢娟; 尹凯; 陈雯; 游咏] 南华大学
关键词：
自主学习;临床技能学;教学资源
摘要：
卓越医生教育培养计划正在深入实施,其中将以学生为中心开展教学、形成学生自主学习能力作为难点着力推进。结合卓医班的改革试点,突出信息技术的应用,支持学生自主学习能力的培养,对临床技能学教学资源进行优化整合,为实现卓医计划项目的实施目标发挥促进作用。
语种：
中文
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Optimalization for pharmacological periconditioning-induced cardioprotection in CABG: Statistical concern for reducing selection bias
作者：
Fu, Li;Liu, Hai;Li, Yiliang*
期刊：
International Journal of Cardiology ,2018年254:342 ISSN：0167-5273
通讯作者：
Li, Yiliang
作者机构：
[Fu, Li] Univ South China, Cent Hosp Loudi, Dept Endocrinol, Inst Clin Med, Loudi 417000, Peoples R China.;[Liu, Hai] Zhengzhou Univ, Dept Cardiac Surg 3, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China.;[Li, Yiliang] Cent S Univ, Xiangya Hosp 3, Dept Neurol, Changsha 410013, Hunan, Peoples R China.;[Li, Yiliang] 138 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Li, Yiliang] 1;138 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.
关键词：
Sample size;Selection bias;Pharmacological periconditioning;CABG
语种：
英文
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Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin
作者：
Zheng, Kang;Xu, Man;Xiao, Yongjian;Luo, Haodang;Xie, Yafeng;...
期刊：
Emerging Microbes & Infections ,2018年7(1):1-10 ISSN：2222-1751
通讯作者：
Wu, Yimou
作者机构：
[Yu, Jian; Wu, Yimou; Zhao, Feijun; Li, Yumeng; Tan, Manyi; Luo, Haodang; Zheng, Kang; Xu, Man; Zeng, Tiebing] Univ South China, Med Coll, Inst Pathogen Biol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Xie, Yafeng; Xiao, Yongjian] Univ South China, Affiliated Hosp 2, Clin Lab, Hengyang 421001, Peoples R China.;[Yu, Jian] Univ South China, Med Coll, Dept Expt Zool, Hengyang 421001, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Med Coll, Inst Pathogen Biol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.
摘要：
Plasmid DNA encoding flagellin FlaB3 was used as a vaccination candidate for the evaluation of immunogenicity and protection against Treponema pallidum subsp. pallidum dissemination. First, intramuscular injection of the flagellin encoded by the plasmid DNA into New Zealand rabbits elicited both humoral and cellular immune responses. Total IgG production increased in response to flagellin. In addition, serum IFN-γ secretion and CD8+ cells were substantially greater in the rabbits immunized with the plasmid encoding flagellin FlaB3 than those in the rabbits immunized with recombinant flagellin. The flagellin encoded by the plasmid DNA induced significant upregulation of serum IL-6 and IL-8 compared to that of the control rabbits. Subsequently, intradermal challenge of the vaccinated New Zealand rabbits with 1 × 10 7 T. pallidum resulted in a significant reduction of the bacterial organ burden in the blood, liver, spleen, and testicles in the flagellin plasmid DNA-vaccinated rabbits. Furthermore, the histopathological analysis demonstrated that the rabbits immunized with the plasmid DNA-encoded flagellin (FlaB3) showed better immune protection. These findings provide evidence that plasmid DNA-encoded flagellin (FlaB3) may be useful as a potential immunization route for future development of a vaccine to inhibit T. pallidum dissemination in related animals. © 2018, The Author(s).
语种：
英文
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Long noncoding RNA cancer susceptibility candidate 9 promotes doxorubicin-resistant breast cancer by binding to enhancer of zeste homolog 2
作者：
Jiang, Baohong;Li, Yuehua* ;Qu, Xiaofei;Zhu, Hongbo;Tan, Yeru;...
期刊：
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE ,2018年42(5):2801-2810 ISSN：1107-3756
通讯作者：
Li, Yuehua
作者机构：
[Jiang, Baohong] Univ South China, Affiliated Hosp 1, Dept Pharm, Hengyang 421001, Hunan, Peoples R China.;[Qu, Xiaofei; Tan, Yeru; Zhu, Hongbo; Li, Yuehua; Jiang, Yiling; Liao, Mingchu; Fan, Qun; Wu, Xiaoping] Univ South China, Affiliated Hosp 1, Dept Med Oncol, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, Yuehua] U;Univ South China, Affiliated Hosp 1, Dept Med Oncol, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
breast cancer;drug-resistance;cancer susceptibility candidate 9;enhancer of zeste homolog 2;multidrug resistance protein 1
摘要：
The present study aimed to investigate the effect of the long noncoding RNA cancer susceptibility candidate 9 (CASC9) on doxorubicin (DOX)-resistant breast cancer and to reveal the potential underlying mechanisms. The expression of CASC9 in breast cancer tissues and cell lines, in addition to drug-resistant breast cancer cells (MCF-7/DOX), was detected by reverse transcription-quantitative polymerase chain reaction. Subsequently, MCF-7/DOX cells were transfected with the silencing vector pS-C ASC9, containing enhancer of zeste homolog 2(EZH2), multidrug resistance protein 1 (MDR1) or control small interfering (si)RNAs. The viability, apoptosis, migration and invasion of the transfected cells were assessed via an MTT assay, flow cytometry and a Transwell assay, respectively. The expression levels of apoptosis-associated proteins (apoptosis regulator Bcl-2, apoptosis regulator BAX, caspase-3 and caspase-9) were determined by western blotting. An RNA pull-down assay was performed to identify CASC9-binding candidates. In addition, the expression levels of the MDR1 gene and its encoded protein, P-glycoprotein, were detected. CASC9 expression was upregulated in breast cancer tissues and cell lines, and drug-resistant breast cancer cells. CASC9 knockdown significantly inhibited the growth and metastasis of drug-resistant breast cancer cells, and decreased the half-maximal inhibitory concentration DOX in MCF-7/DOX cells. The RNA pull-down assay revealed that CASC9 engaged EZH2; EZH2 siRNA significantly inhibited the cell growth, metastasis and chemoresistance of MCF-7/DOX cells. Additionally, EZH2 may regulate the MDR1 gene. The present study demonstrated the oncogenic role of CASC9 in drug-resistant breast cancer by binding to EZH2 and regulating the MDR1 gene. Modulation of CASC9 expression may be a promising target in the therapy of breast cancer and drug-resistant breast cancer. © 2018 Spandidos Publications. All rights reserved.
语种：
英文
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Expression and Purification of Tag-removed Human IL37 by Digestion on Beads in Escherichia coli
作者：
Wu, Daichao;Li, Guoqing;Ma, Yao;Liu, Jin;Li, Yukun;...
期刊：
PROTEIN AND PEPTIDE LETTERS ,2018年25(11):996-1002 ISSN：0929-8665
通讯作者：
Chen, Yongheng;Tan, Sijie;Li, Meixiang
作者机构：
[Li, Yukun; Tan, Sijie; Bai, Junhui; Liu, Jin; Li, Meixiang; Wu, Daichao; Wang, Juan] Univ South China, Hengyang Med Coll, Inst Clin Anat & Reprod Med, Dept Histol & Embryol, Hengyang 421001, Hunan, Peoples R China.;[Wu, Daichao; Li, Guoqing] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Ma, Yao] Univ South China, Network Informat Ctr, Hengyang 421001, Hunan, Peoples R China.;[Chen, Yongheng] Cent S Univ, XiangYa Hosp, Chinese Minist Hlth, Lab Struct Biol,Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.;[Chen, Yongheng] Cent S Univ, XiangYa Hosp, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Chen, Yongheng] C;[Tan, SJ; Li, MX] U;Cent S Univ, XiangYa Hosp, Changsha 410008, Hunan, Peoples R China.;Univ South China, Hengyang Med Coll, Hengyang 421000, Peoples R China.
关键词：
Soluble expression;purification;interleukin 37;Escherichia coli;digestion on beads;inflammatory diseases.
摘要：
Background: Human Interleukin 37 (IL37), a unique anti-inflammatory cytokine of IL1 family member, plays critical roles in innate and adaptive immunity and inflammation. Objective: Preparation of high purity and tag-removed recombinant IL37 protein (rIL37) is critical for its clinical application. Method: In this study, we constructed an N-terminal cleavable GST-fused IL37 expression vector for recombinant expression. Results: Subsequent to transformation and optimization of the induction temperature, the soluble expression level of rIL37 was 306.5 mg/L of culture medium at 18 °C induction in Escherichia coli. Meanwhile, rIL37 was digested on beads by GST-HRV3C protease during GST affinity chromatography. After further purification, the purity of rIL37 was higher than 99 %. Finally, the anti-inflammatory activity of tag-removed protein was verified by the results showing that rIL37 suppressed IL1β production in PBMCs. Conclusion: This work presents a protocol to produce high purity and tag-removed rIL37 with anti-inflammatory activity, which provides the firm basis for advancing clinical application in human IL37-related inflammatory diseases. © 2018 Bentham Science Publishers.
语种：
英文
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Long non-coding RNA MIAT is estrogen-responsive and promotes estrogen-induced proliferation in ER-positive breast cancer cells
作者：
Li, Yuehua;Jiang, Baohong;Wu, Xiaoping* ;Huang, Qin;Chen, Wenqi;...
期刊：
Biochemical and Biophysical Research Communications ,2018年503(1):45-50 ISSN：0006-291X
通讯作者：
Wu, Xiaoping
作者机构：
[Qu, Xiaofei; Zhu, Hongbo; Li, Yuehua; Xie, Liming; Wu, Xiaoping] Univ South China, Affiliated Hosp 1, Dept Med Oncol, 69 Chuanshan Rd, Hengyang City 421001, Hunan, Peoples R China.;[Jiang, Baohong] Univ South China, Affiliated Hosp 1, Dept Pharm, Hengyang City 421001, Hunan, Peoples R China.;[Huang, Qin] Univ South China, Dept Histol & Embryol, Lab Reprod Med, Hengyang City, Hunan, Peoples R China.;[Chen, Wenqi] Univ Hong Kong, Shenzhen Hosp, Dept Oncol, Shenzhen 518000, Peoples R China.;[Ma, Xin] Univ South China, Affiliated Hosp 1, Dept Pathol, Hengyang City, Hunan, Peoples R China.
通讯机构：
[Wu, Xiaoping] U;Univ South China, Affiliated Hosp 1, Dept Med Oncol, 69 Chuanshan Rd, Hengyang City 421001, Hunan, Peoples R China.
关键词：
Breast cancer;Cell cycle;Estrogen receptor;Long non-coding RNA;MIAT;Proliferation
摘要：
Estrogen drives the development and progression of estrogen receptor (ER)-positive breast cancer. However, the detailed mechanism underlying ER-driven carcinogenesis remains unclear despite extensive studies. Previously reports indicated higher expression of long non-coding RNA (lncRNA) myocardial infarction associated transcript (MIAT) in ER-positive breast cancer tissues than in ER-negative tissues. However, the functional relevance of MIAT in ER-positive breast cancer tumorigenesis was poorly understood. Here, we investigated the role of lncRNA MIAT in ER-positive breast cancer cells. MIAT was over-expressed in ER-positive breast cancer tissues and ER-positive breast cancer cell line MCF-7. Activating estrogen signaling by diethylstilbestrol (DES) led to a dose- and time-dependent up-regulation of MIAT in MCF-7 cells that was dependent on ERα as evidenced by ERα silencing and pharmacological inhibition using ER antagonist ICI 182780. Silencing MIAT decreased DES-induced MCF-7 cell proliferation while overexpressing MIAT increased MCF-7 cell proliferation. Further mechanistic study identified that MIAT was critical for G1 to S phase cell cycle transition. Taken together, these results suggest that lncRNA MIAT is an estrogen-inducible lncRNA and a key regulator in ER-positive breast cancer cell growth. MIAT could serve as a potential biomarker and promising therapeutic target for ER-positive breast cancer. © 2018
语种：
英文
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Nucleolin protects against doxorubicin-induced cardiotoxicity via upregulating microRNA-21
作者：
Sun, Hui;Tong, Zhongyi;Fang, Yeqing;Jiang, Bimei* ;Liang, Pengfei;...
期刊：
JOURNAL OF CELLULAR PHYSIOLOGY ,2018年233(12):9516-9525 ISSN：0021-9541
通讯作者：
Jiang, Bimei
作者机构：
[Xiao, Xianzhong; Li, Yuanbin; Tong, Zhongyi; Sun, Hui; Jiang, Bimei; Tang, Yuting] Cent S Univ, Dept Pathophysiol, Sepsis Translat Med Key Lab Hunan Prov, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Sun, Hui] Univ South China, Inst Cardiovasc Dis, Dept Pathophysiol, Hengyang, Hunan, Peoples R China.;[Sun, Hui] Univ South China, Key Lab Arteriosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.;[Tong, Zhongyi] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha, Hunan, Peoples R China.;[Fang, Yeqing] Shenzhen Nanshan Peoples Hosp, Dept Cardiol, Shenzhen, Guangdong, Peoples R China.
通讯机构：
[Jiang, Bimei] C;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410008, Hunan, Peoples R China.
关键词：
cardiotoxicity;doxorubicin (DOX);microRNA-21 (miRNA-21);nucleolin;transgenic (TG) mice.
摘要：
Nucleolin is a multifunctional protein and participates in many important biological processes. Our previous study found that nucleolin protects the heart against myocardial ischemia–reperfusion injury. In this study, we aimed to investigate the role of nucleolin in doxorubicin (DOX)‐induced cardiotoxicity. The expression pattern of nucleolin in hearts subjected to DOX injury was investigated, and we found that administration of DOX induced nucleolin expression significantly in vivo and in vitro. Gene transfection and RNA interference approaches were used in cardiomyocytes to investigate the function of nucleolin. Nucleolin overexpression protects cardiomyocytes against DOX‐induced injury. Nucleolin‐ablated cardiomyocytes become susceptible to the injury induced by DOX. The hearts of cardiac‐myocyte‐specific nucleolin transgenic mice are more resistant to DOX injury. Furthermore, nucleolin upregulates microRNA(miRNA)‐21 expression in vivo and in vitro, and the miRNA‐21 inhibitor negates the protective effect of nucleolin against injury induced by DOX. These results have demonstrated that nucleolin is involved in the regulation of DOX‐induced cardiac injury and dysfunction via the regulation of miRNA‐21 expression, and may be a novel therapeutic target for DOX‐induced cardiotoxicity. Nucleolin is elevated in myocardium in DOX‐treated mice, and nucleolin overexpression protects against DOX‐induced cardiotoxicity. Cardiomyocyte‐specific nucleolin transgenic mice are resistant to DOX‐induced injury. MicroRNA‐21 mediated nucleolin protective effect.
语种：
英文
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Involvement of microRNAs-MMPs-E-cadherin in the migration and invasion of gastric cancer cells infected with Helicobacter pylori
作者：
Yang, Yongmei;Li, Xiaohui;Du, Jie;Yin, Youcong;Li, Yuanjian*
期刊：
Experimental Cell Research ,2018年367(2):196-204 ISSN：0014-4827
通讯作者：
Li, Yuanjian
作者机构：
[Li, Xiaohui; Yang, Yongmei; Yin, Youcong; Li, Yuanjian; Du, Jie] Cent S Univ, Dept Phannacol, Xiangya Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;[Yang, Yongmei] Univ South China, Sch Med, Dept Anat, Hengyang, Hunan, Peoples R China.
通讯机构：
[Li, Yuanjian] C;Cent S Univ, Dept Phannacol, Xiangya Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.
关键词：
*E-cadherin;*Helicobacter pylori;*Invasion;*MMPs;*MicroRNAs;*Migration
摘要：
It has been found that Helicobacter pylori （H. pylori）is not only the main cause of gastric cancer, but also closely related to its metastasis. E-cadherin cleavage induced by matrix metalloproteinases (MMPs) plays an important role in the tumor metastasis. In the present study, we investigated the role of microRNAs-MMPs-E-cadherin in migration and invasion of gastric cancer cells treated with H. pylori. The results showed that H. pylori induced migration and invasion of SGC-7901 cells with a down-regulation of E-cadherin expression, which were abolished by MMPs knock down, E-cadherin overexpression, mimics of miR128 and miR148a. MiR128/miR148a inhibitors restored MMP-3/MMP-7 expression, down-regulated E-cadherin level, and accelerated cellular migration and invasion. This study suggests that H. pylori induces migration and invasion of gastric cancer cells through reduction of E-cadherin function by activation of MMP-3, − 7. The present results also suggest that the activated MMPs/E-cadherin pathway is related with down-regulation of miR128/miR148a in the human gastric cancer cells infected with H. pylori. © 2018
语种：
英文
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PBL在基于卓越医生培养的临床技能教学中的应用
作者：
陈雯;李熠;钟慧;冯聚玲;谢娟;...
期刊：
科教文汇(下旬刊) ,2018年(15):58-60 ISSN：1672-7894
作者机构：
南华大学医学院 湖南·衡阳 421001;[李熠; 钟慧; 谢娟; 赵铁; 冯聚玲; 陈雯] 南华大学
关键词：
基于问题的学习;卓越医生;临床技能教学
摘要：
临床技能教学是我国临床医学本科教育阶段的重要内容,是培养学生自主学习和提高学生职业素养的关键环节,更是每个医学生都必须掌握的基本技能。基于问题的学习（problem-based learning,PBL）是使学生在复杂的临床问题中牢固掌握临床基本技能的学习方法和教学模式之一,在国际上已受到广泛认同,因此在国内医学教育界也受到了提倡。由于PBL遵循＂以学生为本＂的观念,符合现代医学培养模式的思路,总结教师在PBL教学中的定位与职责并阐明教师的多元化作用,有利于推广PBL在卓越医生培养模式下临床技能教学中的应用。
语种：
中文
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多模式信息化教学运用于诊断学教学中的思考与探索
作者：
游咏;李熠;尹凯;杨璐;桂庆军
期刊：
教育现代化 ,2018年5(48):190-191 ISSN：2095-8420
作者机构：
南华大学医学院诊断学教研室,湖南 衡阳;南华大学医学人文教研室,湖南 衡阳;[李熠; 杨璐; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
模式;信息化;诊断学;教学
摘要：
诊断学是医学生必修的一门重要专业课程.如何在诊断学教学过程中选择与构建优化的教学模式与方法,提高教学效果,提升学生的学习兴趣是目前各高校诊断学教学研究的主要方向.本文根据诊断学各章节教学内容的特点,探讨将微课、虚拟仿真以及翻转课堂等信息化教学方式有机运用于诊断学的教学中,有效地促进诊断学教学改革.以期为学生提供丰富的教学资源,提供直观、逼真的学习环境,提高学生动手的机会,锻炼学生的临床思维,调动学生的学习积极性,达到提升诊断学教学质量的目的.
语种：
中文
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微课与翻转课堂有机融合运用于临床医学教学中的思考
作者：
莫靓;沈元琼;尹凯;李熠;冯聚玲;...
期刊：
山西医科大学学报 ,2017年19(5):390-392 ISSN：1007-6611
作者机构：
南华大学附属第一医院胸心外科,衡阳,421001;南华大学医学院诊断学教研室;[李熠; 沈元琼; 冯聚玲; 尹凯; 游咏] 南华大学;[莫靓] 南华大学第一附属医院
关键词：
临床医学;微课;翻转课堂
摘要：
信息技术的飞速发展,使网络课程与学生自主学习的模式成为医学教育发展的必由之路。临床医学教学中医学生的参与至关重要,微课与翻转课堂的有机融合可以更好地促进医学生主动性地学习,充分调动学生学习的积极性及热情,整合各种优质资源,更新学生思想观念,提高医学生的综合素质。
语种：
中文
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Dengue virus and Japanese encephalitis virus infection of the central nervous system share similar profiles of cytokine accumulation in cerebrospinal fluid
作者：
Li, Haipeng;Li, Yuanyuan;Wen, Bo;Zhang, Jian;Wang, Chengwu;...
期刊：
CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY ,2017年42(2):218-222 ISSN：1426-3912
通讯作者：
Huang, Renbin;Liu, Wenpei
作者机构：
[Li, Haipeng; Li, Yuanyuan; Zhang, Jian; Huang, RB; Liu, Wenpei; Huang, Renbin; Wen, Bo; Song, Zhanyi; Li, Shuochi; Wang, Chengwu; Qu, Xiaowang] Univ South China, Peoples Hosp Chenzhou 1, Translat Med Inst, 102 Luojiajing, Chenzhou 423000, Hunan, Peoples R China.;[Li, Yuanyuan] Third Peoples Hosptial Yancheng, Dept Neurol, Yancheng, Jiangsu, Peoples R China.
通讯机构：
[Huang, RB; Liu, WP] U;Univ South China, Peoples Hosp Chenzhou 1, Translat Med Inst, 102 Luojiajing, Chenzhou 423000, Hunan, Peoples R China.
关键词：
Japanese encephalitis virus;central nervous system;cytokine;dengue virus
摘要：
Dengue virus (DENV) and Japanese encephalitis virus (JEV) are two important pathogenic viruses that can cause severe encephalitis, which is accompanied by inflammatory cytokines. However, the inflammatory cytokine content of cerebrospinal fluid (CSF) in DENV and JEV infection of central nervous system are not sufficiently studied. To investigate cytokine levels in serum and CSF of hospitalised children with DENV and JEV infection of the central nervous system, a total of 183 hospitalised children with viral encephalitis-like syndrome were enrolled between May 2014 and April 2015 at the Children’s Hospital of Chenzhou, Hunan, China. DENV and JEV infection was diagnosed by ELISA. Cytokine levels in the serum and CSF were measured by commercial ELISA kits. Twenty-nine (15.85%) and 26 (14.21%) DENV and JEV infections were detected in 183 patients with viral encephalitis-like syndrome, respectively. Higher granulocyte-macrophage colony-stimulating factor (GM-CSF) levels were detected in the serum of JEV infected patients than in DNEV patients (p < 0.05) or in healthy controls (p < 0.001), and levels of GM-CSF, interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were higher in the CSF than serum in both DENV and JEV infection. Both DENV and JEV infection induced similar cytokine accumulation profiles in the CSF, which probably contributed to DENV- and JEV-induced immunopathogenesis. © 2017, Termedia Publishing House Ltd. All rights reserved.
语种：
英文
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