山银花黄酮类提取物对人肝L-02细胞甘油三酯蓄积及SREBP-1c表达的影响
作者：
李熠;匡稳定;周容容;桂庆军;匡双玉
期刊：
中南医学科学杂志 ,2015年(2):145-149 ISSN：2095-1116
作者机构：
南华大学医学院,湖南 衡阳,421001;南华大学附属第二医院;[匡双玉; 周容容; 匡稳定] 南华大学第二附属医院;[李熠; 桂庆军] 南华大学
关键词：
山银花;提取物;黄酮
摘要：
目的观察山银花黄酮类提取物对人肝L-02细胞甘油三酯蓄积的影响,并初步探讨SREBP-1c蛋白及基因表达在其中的作用。方法油红O染色初步观察山银花黄酮类提取物对脂变人肝L-02细胞中脂滴形成的影响。然后以脂变人肝L-02细胞为模型,不同浓度（0、5、10、20 mg/L）山银花黄酮类提取物作用于细胞24 h以及10 mg/L山银花黄酮类提取物作用于细胞不同时间（0、12、24、48、72 h）,生化试剂盒测定细胞内甘油三酯（TG）的含量,Western印迹法检测细胞内SREBP-1c蛋白表达变化,实时荧光定量PCR检测细胞内SREBP-1c mRNA表达变化。结果山银花黄酮类提取物显著降低人肝L-02细胞中脂滴含量,细胞内TG水平下降,SREBP-1c蛋白及基因表达下调,且呈现一定的剂量和时间依赖性。结论山银花中黄酮类提取物能引起人肝L-02细胞TG含量下降,且这种作用与SREBP-1c蛋白及基因表达下调有一定关系。
语种：
中文
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Protective effect of the omega-3 polyunsaturated fatty acids on the schistosomiasis liver fibrosis in mice
作者：
Wu, Yuan;Liang, Yu;Zhu, Yi;Gao, Yongqiang;Chen, Hu;...
期刊：
International Journal of Clinical and Experimental Medicine ,2015年8(6):9470-9476 ISSN：1940-5901
通讯作者：
Xiao, Jianhua
作者机构：
[Liang, Yu; Li, Yingju; Yin, Weiguo; Zhang, Yukuai; Xiao, Jianhua; Gao, Yongqiang; Wu, Yuan; Wang, Kegeng] Univ South China, Coll Med, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.;[Zhu, Yi; Wu, Yuan; Chen, Hu] Univ South China, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Jianhua] Univ South China, Coll Med, Inst Pathogen Biol, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, Jianhua] U;Univ South China, Coll Med, Inst Pathogen Biol, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Schistosoma japonicum;liver fibrosis;protective effect;omega-3 polyunsaturated fatty acids
摘要：
This study aims to observe the effect of ω-3 polyunsaturated fatty acids on initiation and elimination of the schistosomiasis inflammatory response and liver fibrosis. The mice infected with the cercariae of Schistosoma japonicum (20 ± cercarie per mice) were separated randomly into several groups. After 60 days, liver tissue samples of all mice were sectioned. Hematoxylin-eosin (HE) staining, Masson staining, the enzyme-linked immunosorbent assay (ELISA), and flow cytometry (FCM) were performed. Through HE and Masson staining, the size of egg (ovum) granuloma and the collagen deposited in mice’s livers in ω-3 PUFAs and praziquantel mixed groups were less than that of model group and praziquantel treated group. The serum level of IL-13 and TNF-α were lower than that of model group and praziquantel treated group. The indicators of liver fibrosis, such as HA and LN in the group treated with ω-3 PUFAs and praziquantel before the release of soluble eggs antigen (SEA) into blood, were lower than that of model group and praziquantel treated group, respectively. The combined treatment of ω-3 polyunsaturated fatty acids and praziquantel conducted before the release of soluble eggs antigens into the blood decreases liver ovum granulomatous inflammation and fibrosis degree in the schistosomiasis. The mechanism of the ω-3 polyunsaturated fatty acid may be related to the adjustment of the anti-inflammatory and immune responses. © 2015, E-Century Publishing Corporation. All rights reserved.
语种：
英文
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RETRACTED: Betulinic acid downregulates expression of oxidative stress-induced lipoprotein lipase via the PKC/ERK/c-Fos pathway in RAW264.7 macrophages
作者：
Peng, Juan;Lv, Yun-Cheng;He, Ping-Ping;Tang, Yan-Yan;Xie, Wei;...
期刊：
Biochimie ,2015年119:192-203 ISSN：0300-9084
通讯作者：
Yin, Wei-Dong
作者机构：
[Lan, Gan; He, Ping-Ping; Shi, Jin-Feng; Peng, Juan; Zhang, Min; Li, Yuan; Lv, Yun-Cheng; Tang, Chao-Ke; Yin, Wei-Dong; Xie, Wei; Tang, Yan-Yan; Zhang, Chi] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Peng, Juan] Yongzhou Vocat & Tech Coll, Yongzhou 425000, Hunan, Peoples R China.;[He, Ping-Ping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[Liu, Xiang-Yu] Univ South, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Zheng, Xi-Long] Univ Calgary, Dept Biochem & Mol Biol, Libin Cardiovasc Inst Alberta, Cumming Sch Med, Calgary, AB T2N 4N1, Canada.
通讯机构：
[Yin, Wei-Dong] U;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
Atherosclerosis;Betulinic acid;LPL;Oxidative stress
摘要：
Background Atherosclerosis is a major cause of coronary artery disease, which is characterized by cellular lipid accumulation. Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism. Studies have shown that macrophage-derived LPL exhibits proatherogenic properties, and plays a major role in lipid accumulation in macrophages. Evidence suggests that oxidative stress can effectively enhance macrophage LPL production. Betulinic acid (BA) is a pentacyclic lupane triterpene with a potent antioxidant activity. In this study, we investigated whether BA affects the expression of macrophage LPL and how it regulates cellular lipid accumulation. Methods and results We revealed that BA downregulated H2O2-simulated macrophage LPL protein, mRNA levels and its activity in both concentration- and time-dependent manners. Furthermore, BA decreased LPL-involved total cholesterol and triglyceride levels in macrophages. In addition, cellular lipid staining by Oil Red O showed that BA decreased cellular lipid droplet deposition. Next, we confirmed that pretreatment with BA decreased H2O2-induced production of intracellular reactive oxygen species in a concentration-dependent manner. Further studies demonstrated that BA inhibited H2O2-induced membrane translocation of PKC, phosphorylation of ERK1/2 and c-Fos. Finally, the induction of LPL production and activity by H2O2 was abolished by BA, inhibition of PKC or ERK or depletion c-Fos, respectively. Conclusions BA, through its role of antioxidant activity, attenuated macrophage-derived LPL expression and activity induced by oxidative stress, and effectively reduced cellular lipid accumulation, likely through inhibition of the pathways involving PKC, ERK and c-Fos. These effects of BA may contribute to its mitigation of atherosclerosis and help develop BA as a therapeutic compound in treatment of atherosclerosis. © 2015 Published by Elsevier B.V.
语种：
英文
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High-density Lipoprotein Increases the Uptake of Oxidized Low Density Lipoprotein via PPAR gamma/CD36 Pathway in Inflammatory Adipocytes
作者：
Zhong, Qiaoqing;Zhao, Shuiping;Yu, Bilian;Wang, Xing;Matyal, Robina;...
期刊：
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES ,2015年11(3):256-265 ISSN：1449-2288
通讯作者：
Jiang, Zhisheng
作者机构：
[Jiang, Zhisheng; Zhong, Qiaoqing] Univ South China, Postdoctoral Mobile Stn Basic Med, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhisheng; Zhong, Qiaoqing] Univ South China, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Zhong, Qiaoqing] First Peoples Hosp Chenzhou, Dept Cardiol, Chenzhou 423000, Peoples R China.;[Yu, Bilian; Zhong, Qiaoqing; Zhao, Shuiping] Cent S Univ, Xiangya Hosp 2, Dept Cardiol, Changsha 410011, Hunan, Peoples R China.;[Wang, Xing] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Cardiol, Guangzhou 510080, Guangdong, Peoples R China.
通讯机构：
[Jiang, Zhisheng] U;Univ South China, Postdoctoral Mobile Stn Basic Med, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China.
关键词：
Lipoprotein;Atherosclerosis;Inflammation;Lipopolysaccharide
摘要：
Aim: Previous studies have demonstrated that the dysregulated-secretion of adipokines by adipocytes may contribute to obesity-associated atherosclerosis (As) and high density lipoprotein (HDL) may protect against atherogenesis through multiple pathways. This study was to explore the effect of HDL on the oxLDL uptake in inflammatory adipocytes stimulated by endotoxin lipopolysaccharide (LPS) and the possible mechanism. Methods and Results: 3T3-L1 adipocytes were cultured and induced to differentiation and maturation. Acute inflammation in adipocytes was induced by LPS (100 ng/ml) for 6 hours. The adipocytes were pretreated with HDL in various concentrations (10, 50, 100 μg/ml) for 16 hours or with specific PPARγ antagonist (GW9662, 10 μM) or agonist (Rosiglitazone, 10 μM) for 30 min before administration of LPS. The results showed that LPS significantly increased the release of inflammation-related adipokines, such as monocyte chemoattractant protein-1 (MCP-1), plasminogen activator inhibitor 1 (PAI-1), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-8 and IL-6, while decreasing the release of leptin and adiponectin. Meanwhile, LPS reduced the uptake and degradation of 125I-oxLDL, and down-regulated the expression of PPARγ and CD36. Pretreatment with HDL dose-dependently affected the release of IL-8 and IL-6 and the reduced uptake and degradation of oxLDL of adipocytes stimulated by LPS, accompanied with marked upregulation of PPARγ and CD36 expression. Pretreatment with GW9662 markedly inhibited the upregulation of CD36 expression mediated by HDL (100 μg/ml), while the effects of Rosiglitazone were opposite to GW9662. Conclusions: HDL may increase oxLDL uptake of inflammatory adipocytes stimulated by LPS via upregulation of PPARγ/CD36 pathway, which may be a new mechanism of anti-atherosclerosis mediated by HDL. © 2015 Ivyspring International Publisher.
语种：
英文
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Capsaicin attenuates LPS-induced inflammatory cytokine production by upregulation of LXRα
作者：
Tang, Jing;Luo, Kang;Li, Yan;Chen, Quan;Tang, Dan;...
期刊：
International Immunopharmacology ,2015年28(1):264-269 ISSN：1567-5769
通讯作者：
Wang, Deming
作者机构：
[Tang, Jing] Maternal & Child Hlth Hosp Hunan Prov, Dept Anesthesiol, Changsha 410008, Hunan, Peoples R China.;[Luo, Kang; Tang, Dan; Tang, Jing; Li, Yan; Wang, Deming; Chen, Quan; Xiao, Ji] Univ South China, Affiliated Hosp 2, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, Deming] U;Univ South China, Affiliated Hosp 2, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.
关键词：
Capsaicin;Inflammation;LXRalpha;NF-kappaB;PPARgamma
摘要：
Here, we investigated the role of LXR alpha in capsaicin mediated anti-inflammatory effects. Results revealed that capsaicin inhibits LPS-induced IL-1 beta, IL-6 and TNF-alpha production in a time- and dose-dependent manner. Moreover, capsaicin increases LXR alpha expression through PPAR gamma pathway. Inhibition of LXR alpha activation by siRNA diminished the inhibitory action of capsaicin on LPS-induced IL-1 beta, IL-6 and TNF-alpha production. Additionally, LXR alpha siRNA abrogated the inhibitory action of capsaicin on p65 NF-kappa B protein expression. Thus, we propose that the anti-inflammatory effects of capsaicin are LXR alpha dependent, and LXR alpha may potentially link the capsaicin mediated PPAR gamma activation and NF-kappa B inhibition in LPS-induced inflammatory response. (C) 2015 Elsevier B.V. All rights reserved.
语种：
英文
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内皮-间质转化与心血管疾病的研究进展
作者：
Zhang Jing-Jing;You Yong;Li Yin;Gui Qing-Jun;Feng Ju-Ling;...
期刊：
生物化学与生物物理进展 ,2015年42(7):606-615 ISSN：1000-3282
通讯作者：
Yin Kai
作者机构：
[You Yong; Zhang Jing-Jing; Yin Kai; Gui Qing-Jun; Li Yin; Feng Ju-Ling] Univ South China, Sch Med, Res Lab Translat Med, Hengyang 421001, Peoples R China.;[Zhang Jing-Jing] Univ South China, Affiliated Hosp 1, Dept Internal Med Cardiovasc, Hengyang 421001, Peoples R China.;[Yin Kai] Inst Cardiovasc Dis, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
通讯机构：
[Yin Kai] U;Univ South China, Sch Med, Res Lab Translat Med, Hengyang 421001, Peoples R China.
关键词：
内皮-间质转化;心血管疾病;血管重构;心肌纤维化
摘要：
内皮-间质转化（endothelial-to-mesenchymal transition,End MT）属于上皮-间质转化（epithelial-to-mesenchymal transition,EMT）的特殊类型,是内皮细胞在多种刺激因素作用下向间充质细胞转化的过程,在此过程中内皮细胞逐渐失去其形态和功能,获得增殖、迁移和合成胶原等间充质细胞表型特点.近来研究发现,内皮-间质转化在内皮功能调节,心肌、血管及瓣膜的发育和结构重塑等方面发挥着关键的作用,提示其在心血管疾病领域具有重要的研究意义.本文对内皮-间质转化的特点、功能、调节机制以及在心血管系统发育、心肌纤维化、肺动脉高压和动脉粥样硬化性血管重构等心血管疾病中的作用做一综述,以期为心血管疾病的防治提供新靶点.
语种：
中文
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Oxidized Low-Density Lipoprotein Inhibits THP-1-Derived Macrophage Autophagy via TET2 Down-regulation
作者：
Li, Guohua;Peng, Juan;Liu, Yanhui;Li, Xiaohong;Yang, Qin;...
期刊：
Lipids ,2015年50(2):177-183 ISSN：0024-4201
通讯作者：
Wei, Dangheng
作者机构：
[Li, Xiaohong; Liu, Yanhui; Li, Guohua; Tang, Zhihan; Yang, Qin; Li, Yongqing; Jiang, Zhisheng; Peng, Juan; Wang, Zuo; Wei, Dangheng] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
通讯机构：
[Wei, Dangheng] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词：
Plasma lipids;Lipoproteins;Atherosclerosis;Physiology;Oxidized Lipids
摘要：
Oxidized low-density lipoprotein (ox-LDL) is an independent risk factor of atherosclerosis. However, the mechanism underlying its pro-atherosclerosis roles has not yet been well explored. DNA demethylation modification, via DNA methyltransferases or ten-eleven-translocation (TET) family, is a crisis epigenetic regulation for various biological and pathological processes. This study aimed to investigate the effects of ox-LDL on macrophage autophagy and its potential epigenetic mechanism. Results showed that after treatment with 0, 10, 20, 40 or 80 mg/L ox-LDL for 24 h, the autophagy markers Beclin 1 and LC3 expression were obviously decreased at protein levels (P < 0.05). The mRNA and protein expression of TET2 was evidently decreased (P < 0.05). After pre-treatment with TET2 siRNA, the mRNA and protein levels of Beclin 1 and LC3 decreased compared with the 80 mg/L treatment group (P < 0.01). The mRNA and protein levels of Beclin 1 and LC3-II were up-regulated (P < 0.05) in the 5-aza-2′-deoxycytidine (a DNA methyltransferase inhibitor) of pretreatment group. Consistent with the Western blot results, cell immunofluorescence showed that the protein concentration of LC3-II decreased in the TET2 siRNA group and increased in the 5-aza-2′-deoxycytidine group. Taken together, these results showed that DNA demethylation modifications regulate ox-LDL-treated THP-1 macrophages autophagy and TET2 might be a novel regulator.
语种：
英文
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The effects of miR-467b on lipoprotein lipase (LPL) expression, pro-inflammatory cytokine, lipid levels and atherosclerotic lesions in apolipoprotein E knockout mice
作者：
Tian, Guo-Ping;Tang, Yan-Yan;He, Ping-Ping;Lv, Yun-Cheng;Ouyang, Xin-Pin;...
期刊：
Biochemical and Biophysical Research Communications ,2014年443(2):428-434 ISSN：0006-291X
通讯作者：
Yin, Wei-Dong
作者机构：
[Wu, Jian-Feng; Tian, Guo-Ping] Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.;[Wu, Jian-Feng; He, Ping-Ping; Peng, Juan; Zhang, Min; Zhao, Guo-Jun; Li, Yuan; Lv, Yun-Cheng; Tang, Chao-Ke; Ouyang, Xin-Pin; Yin, Wei-Dong; Wang, Jia-Lin; Tang, Yan-Yan] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[He, Ping-Ping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[Cayabyab, Francisco S.; Tang, Shi-Lin] Univ Saskatchewan, Dept Physiol, Saskatoon, SK, Canada.;[Zheng, Xi-Long] Univ Calgary, Libin Cardiovasc Inst Alberta, Hlth Sci Ctr, Dept Biochem & Mol Biol, Calgary, AB T2N 4N1, Canada.
通讯机构：
[Yin, Wei-Dong] U;Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
关键词：
Apolipoprotein E knockout mice;Atherosclerosis;LPL;miR-467b
摘要：
Atherosclerosis is a lipid disorder disease characterized by chronic blood vessel wall inflammation driven by the subendothelial accumulation of macrophages. Studies have shown that lipoprotein lipase (LPL) participates in lipid metabolism, but it is not yet known whether post-transcriptional regulation of LPL gene expression by microRNAs (miRNAs) occurs in vivo. Here, we tested that miR-467b provides protection against atherosclerosis by regulating the target gene LPL which leads to reductions in LPL expression, lipid accumulation, progression of atherosclerosis and production of inflammatory cytokines in apolipoprotein E knockout (apoE-/-) mice. Treatment of apoE-/- mice with intra-peritoneal injection of miR-467b agomir led to decreased blood plasma levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), IL-1β and monocyte chemotactic protein-1 (MCP-1). Using Western blots and real time PCR, we determined that LPL expression in aorta and abdominal cavity macrophages were significantly down-regulated in the miR-467b agomir group. Furthermore, systemic treatment with miR-467b antagomir accelerated the progression of atherosclerosis in the aorta of apoE -/- mice. The present study showed that miR-467b protects apoE -/- mice from atherosclerosis by reducing lipid accumulation and inflammatory cytokine secretion via downregulation of LPL expression. Therefore, targeting miR-467b may offer a promising strategy to treat atherosclerotic vascular disease. © 2013 Elsevier Inc. All rights reserved.
语种：
英文
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Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630
作者：
Chen, Yong;Ding, Yunfeng;Yang, Li;Yu, Jinhai;Liu, Guiming;...
期刊：
Nucleic Acids Research ,2014年42(2):1052-1064 ISSN：0305-1048
通讯作者：
Yu, Jun
作者机构：
[Yang, Fuquan; Huo, Chaoxing; Zhang, Peng; Zhang, Huina; Yang, Li; Ding, Yunfeng; Xie, Zhensheng; Zhang, Shuyan; Xu, Shimeng; Liu, Pingsheng; Chen, Yong; Zhang, Congyan; Wang, Yang; Na, Huimin; Yu, Jinhai] Chinese Acad Sci, Inst Biophys, Natl Lab Macromol, Beijing 100101, Peoples R China.;[Chen, Yong; Zhang, Michael Q.; Li, Yanda] Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Dept Automat,MOE Key Lab Bioinformat, Beijing 100084, Peoples R China.;[Chen, Yong; Zhang, Michael Q.; Li, Yanda] Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Dept Automat,Bioinformat Div, Beijing 100084, Peoples R China.;[Huo, Chaoxing; Zhang, Peng; Yang, Li; Song, Lai; Xu, Shimeng; Zhang, Congyan; Wang, Yang; Zhang, Yue; Na, Huimin; Yu, Jinhai] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Liu, Guiming; Huo, Linhe; Song, Lai; Wang, Xumin; Wang, Guoliang; Yu, Jun; Zhang, Hangxiao; Yu, Dan; Fan, Zhonghua; Zhang, Yue] Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.
通讯机构：
[Yu, Jun] C;Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.
摘要：
Rhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.
语种：
英文
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针对地方医学院校留学生教学的思考
作者：
游咏;李熠;尹凯;沈元琼;桂庆军
期刊：
教育教学论坛 ,2014年(42):148-151 ISSN：1674-9324
作者机构：
南华大学 医学院诊断学教研室,湖南 衡阳,421001;[李熠; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
医学留学生;地方医学院校;教学
摘要：
随着我国留学生教育规模逐步扩大,医学留学生教育质量的好坏已成为评价大学办学水平及提升国际影响力的重要指标之一。本文通过对地方医学院校自身特点的分析,拟从留学生立体化教材建设、师资队伍专项培养及教学方式调整三方面探讨留学生教学在地方医学院校的实施策略,为留学生来华教育可持续发展积累经验。
语种：
中文
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五年制临床医学卓越医生教育培养中医学人文教育的探索
作者：
游咏;田英;桂庆军;沈元琼;李熠;...
期刊：
教育教学论坛 ,2014年(10):153-154 ISSN：1674-9324
作者机构：
南华大学 医学院 诊断学教研室,湖南 衡阳,421001;[李熠; 文格波; 沈元琼; 桂庆军; 尹凯; 游咏; 田英] 南华大学
关键词：
临床医学;卓越医生;医学人文
摘要：
卓越医生教育培养计划的目标是培养高素质医学人才。五年制临床医学本科教育是“5+3”培养模式的关键。本文基于医学人文教育在五年制临床医学卓越医生教育培养中的重要性，拟从课程体系、师资队伍、校园氛围及拓展式教学四个方面探索医学人文教育的实施策略。
语种：
中文
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miR-374b-5p suppresses RECK expression and promotes gastric cancer cell invasion and metastasis
作者：
Xie, Juan;Tan, Zhi-Hui;Tang, Xia;Mo, Ming-Shu;Liu, Yan-Ping;...
期刊：
世界胃肠病学杂志：英文版 ,2014年20(46):17439-17447 ISSN：1007-9327
通讯作者：
Li, Guo-Qing
作者机构：
[Li, Guo-Qing; Xie, Juan; Tang, Xia; Tan, Zhi-Hui; Liu, Yan-Ping] Univ South China, Affiliated Hosp 2, Dept Gastroenterol, Hengyang 421001, Hunan, Peoples R China.;[Xie, Juan; Gan, Run-Liang; Mo, Ming-Shu; Li, Yi] Univ South China, Coll Med, Dept Diagnost, Hengyang 421001, Hunan, Peoples R China.;[Li, Guo-Qing] Univ South China, Affiliated Hosp 2, Dept Gastroenterol, Jiefang Rd 30, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, Guo-Qing] U;Univ South China, Affiliated Hosp 2, Dept Gastroenterol, Jiefang Rd 30, Hengyang 421001, Hunan, Peoples R China.
关键词：
GASTRIC;cancer;micro;RNAS;MICROARRAY;mi;R374b-5p;R;microRNAs microarray;miR-374b-5p;RECK;Invasion and metastasis
摘要：
AIM: To profile expression of microRNAs (miRNAs) in gastric cancer cells and investigate the effect of miR-374b-5p on gastric cancer cell invasion and metastasis.
语种：
英文
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Urotensin II increases foam cell formation by repressing ABCA1 expression through the ERK/NF-κB pathway in THP-1 macrophages
作者：
Wang, Yan;Wu, Jian-Feng;Tang, Yan-Yan;Zhang, Min;Li, Yuan;...
期刊：
Biochemical and Biophysical Research Communications ,2014年452(4):998-1003 ISSN：0006-291X
通讯作者：
Zeng, Gao-Feng
作者机构：
[Wang, Yan] Univ South China, Affiliated Hosp 2, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.;[Li, Yuan; Tang, Chao-Ke; Xie, Wei; Tang, Yan-Yan; Yao, Feng; Zhang, Min] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.;[Wu, Jian-Feng; Chen, Kong; Zeng, Meng-Ya; Zeng, Gao-Feng] Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.;[Zheng, Xi-Long] Univ Calgary, Dept Biochem & Mol Biol, Libin Cardiovasc Inst Alberta, Hlth Sci Ctr, Calgary, AB T2N 4N1, Canada.
通讯机构：
[Zeng, Gao-Feng] U;Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.
关键词：
ABCA1;Cholesterol efflux;ERK;NF-kappaB;Urotensin II
摘要：
Objective Foam cell formation in the arterial wall plays a key role in the development of atherosclerosis. Recent studies showed that Urotensin II (U II) is involved in the pathogenesis of atherosclerosis. Here we examined the effects of human U II on ATP-binding cassette transporter A1 (ABCA1) expression and the underlying mechanism in THP-1 macrophages. Methods and results Cultured THP-1 macrophages were treated with U II, followed by measuring the intracellular lipid contents, cholesterol efflux and ABCA1 levels. The results showed that U II dramatically decreased ABCA1 levels and impaired cholesterol efflux. However, the effects of U II on ABCA1 protein expression and cellular cholesterol efflux were partially reversed by inhibition of extracellular signal regulated kinase 1/2 (ERK1/2) and nuclear factor kappa B (NF-κB) activity, suggesting the potential roles of ERK1/2 and NF-κB in ABCA1 expression, respectively. Conclusion Our current data indicate that U II may have promoting effects on the progression of atherosclerosis, likely through suppressing ABCA1 expression via activation of the ERK/NF-κB pathway and reducing cholesterol efflux to promote macrophage foam cell formation. © 2014 Elsevier Inc. All rights reserved.
语种：
英文
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MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages
作者：
He, Ping-Ping;Ouyang, Xin-Ping;Tang, Yan-Yan;Liao, Li;Wang, Zong-Bao;...
期刊：
Biochimie ,2014年106:81-90 ISSN：0300-9084
通讯作者：
Yin, Wei-Dong
作者机构：
[Huang, Liang; Wu, Jian-Feng; Tang, Yu Lin; Liu, Xiang-Yu; He, Ping-Ping; Ouyang, Xin-Ping; Tian, Guo-Ping; Chen, Wu-Jun; Peng, Juan; Zhang, Min; Zhao, Guo-Jun; Li, Yuan; Lv, Yun-Cheng; Tang, Chao-Ke; Yin, Wei-Dong; Xie, Wei; Tang, Yan-Yan; Yao, Feng] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Liao, Li; He, Ping-Ping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[Ouyang, Xin-Ping] Univ South China, Coll Med, Inst Neurosci, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;[Wang, Zong-Bao; Yin, Wei-Dong] Univ South China, Pharm & Biol Sci Coll, Hengyang 421001, Hunan, Peoples R China.;[Tian, Guo-Ping] Univ South China, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yin, Wei-Dong] U;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
Atherosclerosis;Human THP-1 macrophages;LPL;MiR-590
摘要：
Background: Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting lipid accumulation and inflammatory response. However, the regulation of LPL at the post-transcriptional level by microRNAs has not been fully understood. In this study, we explored whether miR-590 affects the expression of LPL and its potential subsequent effects on lipid accumulation and pro-inflammatory cytokine secretion in human THP-1 macrophages. Methods and results: Using bioinformatics analyses and dual-luciferase reporter assays, we found that miR-590 directly inhibited LPL protein and mRNA expression by targeting LPL 3'UTR. LPL Activity Assays showed that miR-590 reduced LPL activity in the culture media. Oil Red 0 staining and high-performance liquid chromatography assays showed that miR-590 had inhibitory effects on the lipid accumulation in human THP-1 macrophages. We also illustrated that miR-590 alleviated pro-inflammatory cytokine secretion in human THP-1 macrophages as measured by ELISA. With the method of small interfering RNA, we found that LPL siRNA can inhibit the miR-590 inhibitor-induced increase in lipid accumulation and secretion of pro-inflammatory cytokines in oxLDL-treated human THP-1 macrophages. Conclusions: MiR-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting LPL gene in human THP-1 macrophages. Therefore, targeting miR-590 may offer a promising strategy to treat atherosclerotic cardiovascular diseases. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved.
语种：
英文
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ECS模拟人在卓越医师临床技能教学中的探索与应用
作者：
钟慧;漆辉洲;沈元琼;莫明树;李熠
期刊：
教育教学论坛 ,2014年(45):133-135 ISSN：1674-9324
作者机构：
南华大学医学院,湖南 衡阳,421001;中山大学附属第一医院 神经内科,广东 广州,510080;[李熠; 沈元琼; 漆辉洲; 钟慧] 南华大学;[莫明树] 中山大学附属第一医院
关键词：
卓越医师;临床技能教学
摘要：
目的:分析ECS模拟人在卓越医师临床技能教学中的教学方式和效果。方法:使用ECS模拟人对我校2010级临床医学专业卓越医师班26名学生进行教学训练,另取同班级26名进行传统课堂教学模式的学生为对照组,观察教学效果并进行问卷调查。结果:教学前两组学生考核成绩无明显差别,经ECS教学后实验组学生对临床技能的掌握程度显著提高（P<0.05）,其整体成绩也显著高于对照组（P<0.05）。结论:ECS模拟人教学有助于提高学生的临床操作能力。
语种：
中文
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Lipoprotein lipase: From gene to atherosclerosis
作者：
Li, Yuan;He, Ping-Ping;Zhang, Da-Wei;Zheng, Xi-Long;Cayabyab, Fracisco S.;...
期刊：
Atherosclerosis ,2014年237(2):597-608 ISSN：0021-9150
通讯作者：
Yin, Wei-Dong
作者机构：
[Li, Yuan; Tang, Chao-Ke; He, Ping-Ping; Yin, Wei-Dong] Univ South China, Life Sci Res Ctr, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Cardiovasc Res,Key Lab Atherosclerol Hunan P, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Da-Wei] Univ Alberta, Dept Pediat, Edmonton, AB T6G 2S2, Canada.;[Zhang, Da-Wei] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada.;[Zheng, Xi-Long] Univ Calgary, Hlth Sci Ctr, Cumming Sch Med, Dept Biochem & Mol Biol,Libin Cardiovasc Inst Alb, Calgary, AB T2N 4N1, Canada.;[Cayabyab, Fracisco S.] Univ Saskatchewan, Coll Med, Dept Surg, Saskatoon, SK S7N 0W0, Canada.
通讯机构：
[Yin, Wei-Dong] U;Univ South China, Life Sci Res Ctr, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Cardiovasc Res,Key Lab Atherosclerol Hunan P, Hengyang 421001, Hunan, Peoples R China.
关键词：
Atherosclerosis;Inflammation;LPL;Lipid;Lipoprotein;Triglyceride
摘要：
Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and responsible for catalyzing lipolysis of triglycerides in lipoproteins. LPL is produced mainly in adipose tissue, skeletal and heart muscle, as well as in macrophage and other tissues. After synthesized, it is secreted and translocated to the vascular lumen. LPL expression and activity are regulated by a variety of factors, such as transcription factors, interactive proteins and nutritional state through complicated mechanisms. LPL with different distributions may exert distinct functions and have diverse roles in human health and disease with close association with atherosclerosis. It may pose a pro-atherogenic or an anti-atherogenic effect depending on its locations. In this review, we will discuss its gene, protein, synthesis, transportation and biological functions, and then focus on its regulation and relationship with atherosclerosis and potential underlying mechanisms. The goal of this review is to provide basic information and novel insight for further studies and therapeutic targets. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
语种：
英文
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提升师资队伍综合实力建设 促进卓越医生的培养
作者：
谢娟;尹凯;冯聚玲;程健琳;钟慧;...
期刊：
医学教育研究与实践 ,2014年(4):765-767 ISSN：2096-3181
作者机构：
南华大学医学院诊断学教研室,湖南衡阳,421001;南华大学预防医学与放射卫生实验中心,湖南衡阳,421001;[李熠; 程健琳; 沈元琼; 钟慧; 谢娟; 冯聚玲; 尹凯; 游咏] 南华大学
关键词：
卓越医生;师资队伍建设;综合实力
摘要：
卓越医生的培养是从培养卓越医学生做起，建设一支高素质的高校师资队伍是培养卓越医学生的根本保证。本文对提高师资队伍综合实力建设方面提出了几点建议，即提高人文素养，树立教师道德模范；多途径、全方位提高教师教学能力；着力解决教师的实际问题，创造良好的工作环境。
语种：
中文
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番茄红素对大鼠脑缺血再灌注损伤的保护作用及脑组织caspase-3表达的影响
作者：
匡稳定;匡双玉;周容容;李熠
期刊：
山东医药 ,2014年(27):36-38 ISSN：1002-266X
作者机构：
南华大学附属第二医院,湖南衡阳,421001;南华大学医学院诊断学教研室;[匡双玉; 周容容; 匡稳定] 南华大学第二附属医院;[李熠] 南华大学
关键词：
番茄红素;脑缺血再灌注损伤;天冬氨酸特异性半胱氨酸蛋白酶
摘要：
目的：观察番茄红素对大鼠脑缺血再灌注损伤的保护作用及脑组织天冬氨酸特异性半胱氨酸蛋白酶（ caspase ）-3表达的影响。方法选择雄性SD大鼠36只，利用线栓法制备SD大鼠大脑中动脉缺血模型，模型制备成功后随机分为3组各12只，对照组以羧甲基纤维素钠溶液灌胃，番茄红素组以40 mg/kg番茄红素（羧甲基纤维素钠悬溶）灌胃，银杏叶片组以40 mg/kg银杏叶片（羧甲基纤维素钠悬溶）灌胃，共14 d。按照Bederson6级5分评分标准评估各组大鼠神经行为学，显微镜下观察大鼠脑组织形态结构，Western blotting法检测脑组织caspase-3。结果番茄红素组、银杏叶片组、对照组干预14 d神经功能缺损程度评分分别为（2．25±0．17）、（2．04±0．28）、（3．76±0．42）分，番茄红素组、银杏叶片组与对照组比较，P均＜0．05。对照组神经组织大片坏死，周围残存组织结构不完整，神经细胞水肿明显，大量空泡形成；番茄红素组与银杏叶片组神经组织结构明显改善、坏死区域缩小，梗死灶周围残存组织较多。番茄红素组、银杏叶片组、对照组caspase-3相对表达值分别为0．2、0．15、1，番茄红素组、银杏叶片组与对照组比较，P均＜0．05。结论番茄红素对大鼠脑缺血再灌注损伤有一定的保护作用，且能引起脑组织caspase-3表达下调。
语种：
中文
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菝葜乙酸乙酯提取物对THP-1源性荷脂细胞胆固醇蓄积及SREBP-1表达的影响
作者：
匡双玉;李熠;匡稳定;刘塔斯
期刊：
中国实验方剂学杂志 ,2014年20(3):137-140 ISSN：1005-9903
作者机构：
湖南中医药大学药学院,长沙 410007;南华大学附属第二医院,湖南衡阳 421001;南华大学医学院,湖南衡阳,421001;南华大学附属第二医院,湖南衡阳,421001;湖南中医药大学药学院,长沙,410007
关键词：
菝葜;乙酸乙酯提取物;胆固醇;类固醇调节因子结合蛋白-1
摘要：
目的:观察菝葜乙酸乙酯提取物对人单核/巨噬细胞系（THP-1）源性荷脂细胞胆固醇蓄积的影响,并初步探讨类固醇调节因子结合蛋白-1（SREBP-1）蛋白表达在其中的作用。方法:以THP-1源性荷脂细胞为模型,洛伐他汀（80 mg·L-1）做阳性对照,菝葜乙酸乙酯提取物不同浓度（0,40,80,160 mg·L-1）作用于细胞24 h以及80 mg·L-1作用于细胞不同时间（0,12,24,48 h）,高效液相色谱法测定细胞内总胆固醇（TC）,游离胆固醇（FC）,胆固醇酯（CE）的含量,油红O染色观察菝葜乙酸乙酯提取物对THP-1源性荷脂细胞中脂滴形成的影响,Western blot印迹法检测细胞内SREBP-1蛋白表达变化。结果:菝葜乙酸乙酯提取物明显降低THP-1源性荷脂细胞中脂滴含量,细胞内TC,FC,CE水平呈现一定的剂量和时间依赖性下降趋势,同时SREBP-1蛋白表达上调。结论:菝葜乙酸乙酯提取物能引起THP-1源性荷脂细胞TC,FC,CE含量下降,且这种作用与SREBP-1蛋白表达上调有一定关系。
语种：
中文
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薯蓣皂苷对缺血再灌注大鼠脑损伤及caspase-3表达的影响
作者：
匡双玉;李熠;匡稳定;游咏;桂庆军;...
期刊：
中国医院药学杂志 ,2014年34(9):692-695 ISSN：1001-5213
作者机构：
[匡双玉] 湖南中医药大学药学院;[匡稳定; 刘塔斯] 南华大学附属第二医院;[李熠; 游咏; 桂庆军] 南华大学医学院
关键词：
薯蓣皂苷;缺血再灌注;脑损伤
摘要：
目的:观察薯蓣皂苷对大鼠缺血再灌注脑损伤的保护作用,同时检测在此过程中脑组织caspase-3蛋白表达的变化。方法:利用线栓法制作SD大鼠大脑中动脉缺血(MCAO)模型,根据完全随机原则分为5组,分别为假手术组、模型组及薯蓣皂苷不同剂量(40,80,160 mg·kg~(-1)·d~(-1),ig)治疗组。14 d后,进行神经行为学评分,观察脑组织形态结构,检测血清TNF-α浓度,测定脑组织caspase-3蛋白的表达。结果:在薯蓣皂苷作用下,缺血再灌注模型大鼠神经行为缺损得到一定程度改善,脑组织坏死区域缩小,血清TNF-α浓度下降,脑组织caspase-3蛋白表达降低,并呈现一定的剂量依赖性。结论:薯蓣皂苷对大鼠缺血再灌注脑损伤有保护作用,且能引起缺血再灌注脑损伤大鼠脑组织caspase-3蛋白表达下调。
语种：
中文
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