糖尿病对冠脉侧支循环的影响及与血清ADMA浓度的关系
作者：
游咏;莫靓;桂庆军;尹凯;李熠;...
期刊：
中南医学科学杂志 ,2012年40(1):73-75 ISSN：2095-1116
作者机构：
南华大学医学院诊断学教研室,湖南衡阳,421001;[李熠; 莫靓; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
糖尿病;冠状动脉;侧支循环
摘要：
目的 探讨糖尿病对严重冠脉狭窄患者侧支循环形成的影响以及与血清ADMA浓度的关系. 方法 收集主要冠脉至少有一支狭窄程度在95％以上的112例冠心病患者,分为糖尿病与非糖尿病组,测定其血清ADMA浓度.采用Rentrop法对冠脉侧支循环进行分级.同时测定两组血清ADMA浓度. 结果 糖尿病组侧支循环形成不良的比例高于非糖尿病组(P=0.043,P＜0.05),其血清ADMA浓度也显著高于非糖尿病组(P=0.000,P＜0.01)；合并糖尿病的侧支循环不良患者血清ADMA浓度显著高于无糖尿病患者(P=0.001,P＜0.01).结论 糖尿病抑制严重冠脉狭窄的冠心病患者侧支循环的形成,且与患者血清ADMA浓度相关.
语种：
中文
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PBL教学法与人文素质教育结合在临床技能教学中的运用探讨
作者：
游咏;莫靓;桂庆军;尹凯;李熠;...
期刊：
医学教育研究与实践 ,2011年19(3):509-511 ISSN：2096-3181
作者机构：
南华大学,医学院诊断学教研室,湖南,衡阳,421001;[王北冰; 李熠; 莫靓; 沈元琼; 涂玉林; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
临床技能教学;人文素质教育
摘要：
临床技能教学作为现代医学教育的重要内容,是实现培养具备初步临床能力、终身学习能力和良好职业素质的医学生的关键环节。PBL教学法与人文素质教育结合,按照"以学生为本"的观念改革教学方法,更有利于培养医学生的临床实践能力,符合现代医学模式下培养医学生的改革思路。可为临床技能教学改革提供借鉴。
语种：
中文
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严重冠脉狭窄患者血清二甲基精氨酸水平与侧支循环形成的关系
作者：
游咏;莫靓;桂庆军;尹凯;李熠;...
期刊：
中国老年学杂志 ,2011年31(13):2454-2455 ISSN：1005-9202
作者机构：
[游咏; 莫靓; 桂庆军; 尹凯; 李熠; 李传昶; 杨天伦] 南华大学医学院诊断学教研室
关键词：
冠状动脉;侧支循环;非对称性二甲基精氨酸
摘要：
目的探讨严重冠脉狭窄患者侧支循环形成与否与血清二甲基精氨酸（ADMA）浓度的关系。方法经冠状动脉造影收集主要冠脉至少有一支狭窄程度在95%以上的123例冠心病患者,测定其血清ADMA浓度。采用Rentrop方法对冠状动脉侧支循环进行分级,0级为侧支循环未形成组（n=63）,13级为侧支循环形成组（n=60）。结果侧支循环未形成组血清ADMA浓度（2.43±0.78）μmol/L显著高于侧支循环形成组（1.95±0.72）μmol/L（P<0.01）。结论严重冠脉狭窄患者血清ADMA浓度与侧支循环形成有关。
语种：
中文
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Effects of Aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats
作者：
Li, Qiangxiang;Ao, Xiang;Du, Youhong;Li, Yang;Ou, Yangshi;...
期刊：
ENDOCRINE ,2011年39(3):251-258 ISSN：1355-008X
通讯作者：
Wen, Gebo
作者机构：
[Wen, Gebo; Li, Qiangxiang] NanHua Univ, Affiliated Hosp 1, Clin Res Inst, Henyang 421001, Hunan, Peoples R China.;[Ao, Xiang] Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Du, Youhong; Li, Qiangxiang; Gong, Raofeng; Sun, Xuexiong; Yang, Yi Xiang] Loudi Municipal Cent Hosp Hunan, Loudi 417000, Peoples R China.;[Li, Yang] Maternal & Child Hlth Hosp Hunan Prov, Changsha 410008, Hunan, Peoples R China.;[Ou, Yangshi] Liberat Army 458 Hosp Guangdong Prov, Guangzhou 510602, Guangdong, Peoples R China.
通讯机构：
[Wen, Gebo] N;NanHua Univ, Affiliated Hosp 1, Clin Res Inst, Henyang 421001, Hunan, Peoples R China.
关键词：
Aminoguanidine;Vitamin C;Diabetic nephropathy;Type IV collagen
摘要：
The aim of this article is to investigate the effects of Aminoguanidine and vitamin C (VitC) on type IV collagen in diabetic nephropathy rats. Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were randomly divided into five groups: normal control group (n = 10), diabetes group (n = 10), aminoguanidine group (n = 10), VitC group (n = 10), aminoguanidine and VitC group (n = 10). After 16 weeks, the general conditions, blood gloucose, glycosylated hemoglobin, blood urea nitrogen, serum creatinine, serum type IV collagen, urinary albumin excretion rate, and creatinine clearance rate were detected, type IV collagen protein was determined by immunohistochemical analysis as well as the expression of collagen type IVα1 mRNA were determined by in situ hybridization analysis in the kidneys of each group. The results were (1) diabetes mellitus and renal lesions occurred in the diabetes group, aminoguanidine group, VitC group, VitC and aminoguanidine group; (2) aminoguanidine and VitC improved the general conditions of diabetic nephropathy rats, decreased blood urea nitrogen, serum creatinine, and urinary albumin excretion rate as well as increased creatinine clearance rate. The expressions of collagen type IV were significantly down-regulated in treatment groups in contrast to the diabetes group. Aminoguanidine and VitC protect renal lesions in diabetic nephropathy, respectively, by inhibiting expression of type IV collagen, while aminoguanidine and VitC have a synergistic effect on them. © Springer Science+Business Media, LLC 2010.
语种：
英文
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Identification of phage display peptides with affinity for the tegument of Schistosoma japonicum schistosomula
作者：
Liu, Yan;Brindley, Paul J.;Zeng, Qingren* ;Li, Yuesheng;Zhou, Jun;...
期刊：
Molecular and Biochemical Parasitology ,2011年180(2):86-98 ISSN：0166-6851
通讯作者：
Zeng, Qingren
作者机构：
[Liu, Yan; Yang, Shenghui; Zhang, Zuping; Cai, Liting; Zeng, Qingren; Chen, Yuxiao; Li, Yuesheng; Liu, Biyuan] Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha 410013, Hunan, Peoples R China.;[Brindley, Paul J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol & Trop Med, Washington, DC 20037 USA.;[McManus, Donald P.; Li, Yuesheng] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld 4006, Australia.;[Liu, Yan] Univ S China, Sch Med, Dept Parasitol, Hengyang 421001, Peoples R China.;[Zhou, Jun] Cent S Univ, Xiangya Hosp 3, Expt Ctr, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Zeng, Qingren] C;Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Tongzipo Rd 172, Changsha 410013, Hunan, Peoples R China.
关键词：
Ectogenic polypeptide;M13 phage peptide ZL4 (MppZL4);Phage display technology;Schistosoma japonicum;Schistosomicidal effect
摘要：
Peptides, bound to the tegument of live Schistosoma japonicum schistosomula, were differentially screened by phage display in vitro using three rounds of reverse absorption and bio-panning. Three M13 phage peptides were isolated and identified by determination of their recovery rate, immunohistochemical localization, immunoblot analysis, and their anti-schistosomal effects in vivo and in vitro. Of the three, M13 phage peptide ZL4 (MppZL4, YSGLQDSSLRLR, 1.4 kDa, pI 8.8) bound to the tegument of mechanically transformed schistosomula and to other developmental stages of S. japonicum from the mammalian host. By contrast, MppZL4 did not bind to the surface of cercariae. To further examine its binding properties, MppZL4 was conjugated to Rhodamine B (RhB-YSGLQDSSLRLR, RhB-ZL4) and a peptide control (RhB-AIPYFSGILQWR, RhB-12P) was similarly synthesized. The binding capacities of RhB-ZL4 to the surface membrane of S. japonicum schistosomula in vitro and of S. japonicum adult worms in vivo were examined and revealed specificity for binding. When examined for anti-parasite activity, both MppZL4 and RhB-ZL4 exhibited a potent schistosomicidal effect in vitro. Further MppZL4 also affected the growth and development of schistosomula in vivo. These findings extend previous studies showing that phage display techniques can recover polypeptides that bind specifically to living schistosomes and, moreover, that these bound peptides have the potential to inhibit key physiological processes in these parasites. Our findings suggest further that ectogenic polypeptides, which can bind to the tegument of S. japonicum, might be adapted as vectors to deliver experimental probes and/or pharmacologically relevant compounds to the schistosome tegument, including drugs and immunological mediators. © 2011 2011 Elsevier B.V. All rights reserved.
语种：
英文
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Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes
作者：
Yang, Shenghui;Brindley, Paul J.;Zeng, Qingren* ;Li, Yuesheng;Zhou, Jun;...
期刊：
Molecular and Biochemical Parasitology ,2010年174(2):109-116 ISSN：0166-6851
通讯作者：
Zeng, Qingren
作者机构：
[Yang, Shenghui; Liu, Yan; Cai, Liting; Wei, Qi; Zeng, Qingren; Li, Yuesheng; Liu, Biyuan; Zeng, Tiebing; Lan, Lingmei] Cent South Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha 410013, Hunan, Peoples R China.;[Brindley, Paul J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol &Trop Med, Washington, DC 20037 USA.;[McManus, Donald P.; Li, Yuesheng] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld 4006, Australia.;[Yang, Shenghui] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha 410208, Hunan, Peoples R China.;[Zhou, Jun] Cent South Univ, Xiangya Hosp 3, Expt Ctr, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Zeng, Qingren] C;Cent South Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.
关键词：
Glycoprotein of vesicular stomatitis virus (VSVG);Murine leukemia virus;Pantropic retrovirus;Schistosoma japonicum;Telomerase;Transgene
摘要：
Although draft genome sequences of two of the major human schistosomes, Schistosoma japonicum and Schistosoma mansoni are available, the structures and characteristics of most genes and the influence of exogenous genes on the metabolism of schistosomes remain uncharacterized. Furthermore, which functional genomics approaches will be tractable for schistosomes are not yet apparent. Here, the vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse transcriptase gene (hTERT) as a reporter, under the control of the retroviral long terminal repeat (LTR). Pseudotyped virions were employed to transduce S. japonicum to investigate the utility of retrovirus-mediated transgenesis of S japonicum and the activity of human telomerase reverse transcriptase as a reporter transgene in schistosomes. Schistosomules perfused from experimentally infected rabbits were cultured for 6 days after exposure to the virions after which genomic DNAs from virus exposed and control worms were extracted. Analysis of RNA from transduced parasites and immunohistochemistry of thin parasite sections revealed expression of hTERT in the transduced worms. Expression of hTERT was also confirmed by immunoblot analysis. These findings indicated that S. japonicum could be effectively transduced by VSVG-pseudotyped retrovirus carrying the hTERT gene. Given the potential of hTERT to aid in derivation of immortalized cells, these findings suggest that this pantropic retroviral approach can be employed to transduce cells from specific tissues and organs of schistosomes to investigate the influence of transgene hTERT on growth and proliferation of schistosome cells. (C) 2010 Elsevier B.V. All rights reserved.
语种：
英文
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脂联素抑制晚期糖基化终末产物诱导的HUVEC-12细胞CD_(40)高表达
作者：
邓翔;沈元琼;游咏;梁丽红;李熠;...
期刊：
中国现代医学杂志 ,2010年20(17):2612-2615 ISSN：1005-8982
作者机构：
南华大学附属第一医院,湖南衡阳,421001;南华大学医学院,湖南衡阳,421001
关键词：
脂联素;晚期糖基化终末产物;动脉粥样硬化
摘要：
目的研究脂联素（APN）对晚期糖基化终末产物（AGEs）诱导HUVEC-12细胞CD40高表达的干预作用。方法①用不同浓度AGE（0、100、200、400和800mg/L）处理细胞24h,采用实时定量PCR和Western Blot技术分别检测HUVEC-12细胞CD40 mRNA和蛋白质的表达。②用不同浓度APN（0、5、10和15μg/mL）预处理细胞,加入AGE（s400mg/L）共同培养细胞24h,采用实时定量PCR和Western Blot技术检测HUVEC-12细胞CD40 mRNA和蛋白质的表达。结果①不同浓度AGEs处理细胞24h,随着处理浓度增大,HUVEC-12细胞CD40的表达逐渐上调;②不同浓度APN预处理细胞后,随着浓度增大,APN逐渐下调AGEs诱导的HUVEC-12细胞CD40高表达,对正常状态下的CD40表达没有影响。结论 AGEs诱导HU-VEC-12细胞CD40表达上调,脂联素具有抑制AGEs诱导HUVEC-12细胞CD40高表达的作用。
语种：
中文
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Recombinant Vascular Basement Membrane Derived Multifunctional Peptide Blocks Endothelial Cell Angiogenesis and Neovascularization
作者：
Wang, Chengkun;Cao, Jianguo;Qu, Jiaquan;Li, Yafei;Peng, Bo;...
期刊：
JOURNAL OF CELLULAR BIOCHEMISTRY ,2010年111(2):453-460 ISSN：0730-2312
通讯作者：
He, Zhimin
作者机构：
[Peng, Bo; Wang, Chengkun; He, Zhimin; Li, Yafei; Gu, Yixue] Cent S Univ, Canc Res Inst, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China.;[Qu, Jiaquan; Wang, Chengkun; Cao, Jianguo] Univ S China, Canc Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Cao, Jianguo] Hunan Normal Univ, Coll Med, Changsha 410006, Hunan, Peoples R China.;[He, Zhimin] Cent S Univ, Canc Res Inst, Xiangya Sch Med, Xiangya Rd 110, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[He, Zhimin] C;Cent S Univ, Canc Res Inst, Xiangya Sch Med, Xiangya Rd 110, Changsha 410078, Hunan, Peoples R China.
关键词：
RECOMBINANT VASCULAR BASEMENT MEMBRANE DERIVED MULTIFUNCTIONAL PEPTIDE;ANGIOGENESIS INHIBITORS;ENDOTHELIAL CELL;NEOVASCULARIZATION
摘要：
Angiogenesis is an innovative target in the therapy of cancer and other diseases, but the effects of anti-angiogenic drugs have been rather modest in clinical trials. We have developed a small peptide, recombinant vascular basement membrane derived multifunctional peptide (rVBMDMP), which significantly inhibits endothelial cells in vitro. Here we test the mechanisms of rVBMDMP in angiogenesis balance in assays of tubule formation, colony formation, and apoptosis in HUVE-12 endothelial cells. We also analyzed the differential expression of phosphorylation proteins and related genes in a protein phosphorylation chip and extracellular matrix adhesion molecule cDNA microarray, and validated changes with Western blot or real-time quantitative PCR, respectively. rVBMDMP dose-dependently inhibited colony formation, induced apoptosis, and inhibited in vitro tubule formation. rVBMDMP increased the phosphorylation of 88 signal proteins, including caspase-3, death receptor 3, 4, and 5, and integrin alpha V, beta 1, and beta 3, and down-regulated 41 signal proteins, including EGFR, pEGFR, VEGFR-1, and survivin versus control. rVBMDMP upregulated 14 genes, including collagen 4, 7, and 27, and down-regulated 21 genes, including integrin alpha V beta 3, MMP10, and MMP12. Our study suggests that rVBMDMP inhibits angiogenesis and may be a viable drug candidate in anti-angiogenesis and anticancer therapies. J. Cell. Biochem. 111: 453-460, 2010. (C) 2010 Wiley-Liss, Inc.
语种：
英文
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姜黄素对巨噬细胞源性荷脂细胞胆固醇水平及caveolin-1表达的影响
作者：
李熠;匡双玉;王北冰;尹凯;沈元琼;...
期刊：
中南医学科学杂志 ,2009年(01):18-20 ISSN：2095-1116
作者机构：
1. 南华大学医学院诊断学教研室;2. 南华大学第二附属医院药剂科
关键词：
姜黄素;巨噬细胞;荷脂细胞;胆固醇
摘要：
目的观察姜黄素对巨噬细胞源性荷脂细胞胆固醇水平的影响,并初步探讨caveolin-1蛋白表达在其中的作用。方法实验分组分为正常巨噬细胞组、巨噬细胞源性荷脂细胞组和姜黄素处理组。3组均采用油红O染色检测细胞荷脂情况,高效液相色谱分析法检测细胞内胆固醇含量,Western印迹法检测caveolin-1蛋白的表达。结果姜黄素作用于巨噬细胞源性荷脂细胞,细胞内总胆固醇、游离胆固醇和胆固醇酯含量均下降;caveolin-1蛋白表达上调。结论姜黄素引起巨噬细胞源性荷脂细胞胆固醇含量下降的同时,caveolin-1蛋白表达上调。
语种：
中文
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医学生临床基本技能培养新模式的探讨
作者：
李熠;桂庆军;尹凯;游咏;沈元琼
期刊：
医学教育研究与实践 ,2009年17(6):1268-1270 ISSN：2096-3181
作者机构：
南华大学,医学院临床技能教学中心,湖南,衡阳,421001
关键词：
医学生;临床基本技能;教学改革
摘要：
医学生临床基本技能是评价医科教育质量的重要指标,本文从构建科学的课程体系、改革教学方法、改革实践教学模式、因材施教、医学人文教育等方面探讨了临床基本技能培养的新模式。
语种：
中文
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依达拉奉联合纳洛酮治疗急性脑梗死疗效观察
作者：
曾琳;文兰;李熠
期刊：
中南医学科学杂志 ,2009年37(2):200-202 ISSN：2095-1116
作者机构：
南华大学,第一附属医院,神经内科,湖南,衡阳,421001;南华大学,第一附属医院,临床教学办;南华大学,医学院,诊断学教研室;[李熠] 南华大学;[曾琳; 文兰] 南华大学第一附属医院
关键词：
依达拉奉;纳洛酮;急性脑梗死
摘要：
目的 观察依达拉奉联合纳洛酮治疗急性脑梗死的临床疗效. 方法 将90例急性脑梗死患者随机分成治疗组和对照组,每组45人.对照组予常规治疗,治疗组在此基础上加用依达拉奉和纳洛酮治疗,比较治疗前、治疗后2周临床疗效及神经功能缺损评分(NDS). 结果 治疗组的显效率和总有效率明显高于对照组 (P<0.05),治疗组NDS减少程度显著高于对照组(P<0.01). 结论 依达拉奉联合纳洛酮治疗急性脑梗死疗效确切,安全性高.
语种：
中文
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喘乐宁氧气雾化吸入治疗COPD急性加重期疗效观察
作者：
陈小红;肖明;桂庆军;尹凯;李熠
期刊：
中南医学科学杂志 ,2008年36(3):375-377 ISSN：2095-1116
作者机构：
[肖明; 陈小红] 永州职业技术学院附属医院;南华大学医学院;[李熠; 桂庆军; 尹凯] 南华大学
关键词：
慢性阻塞性肺疾病;喘乐宁;血气分析
摘要：
目的 观察氧气雾化吸入喘乐宁治疗慢性阻塞性肺疾病(COPD)急性加重期患者的疗效.方法 62例COPD急性加重期患者随机分为对照组(28例)和治疗组(34例).两组均予吸氧、抗感染、祛痰、解痉等对症治疗.治疗组加用喘乐宁氧气雾化吸入.观察两组患者治疗前后症状、体征及动脉血气变化.结果 治疗组治疗总有效率高于对照组(P<0.05),血气指标的改善优于对照组(P<0.05).结论 氧气雾化吸入喘乐宁是治疗COPD急性加重期的一种有效方法.
语种：
中文
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优化组合多媒体教学方法提高诊断学教学质量
作者：
李熠;桂庆军;王北冰;沈元琼
期刊：
医学教育研究与实践 ,2008年16(3):573-574 ISSN：2096-3181
作者机构：
南华大学,医学院诊断学教研室,湖南,衡阳,421001
关键词：
优化组合;多媒体教学;诊断学
摘要：
通过探讨诊断学多媒体教学方法的发展历程及特点,分析各种多媒体教学方法的优缺点,提出通过优化组合多媒体教学方法,可以实现优势互补,相辅相成,从而提高教学质量.
语种：
中文
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幽门螺杆菌清除治疗对反流性食管炎疗效的影响
作者：
肖明;周柏林;桂庆军;王北冰;沈元琼;...
期刊：
中南医学科学杂志 ,2006年34(4):569-571 ISSN：2095-1116
作者机构：
[肖明] 永州职业技术学院附属医院;[周柏林] 湖南省东安县人民医院;南华大学医学院;[陈扬勤; 王北冰; 李熠; 沈元琼; 桂庆军; 谢翠娥; 尹凯] 南华大学
关键词：
幽门螺杆菌;反流性食管炎;治疗
摘要：
目的 探讨幽门螺杆菌(Hp)清除治疗对反流性食管炎(RE)疗效的影响.方法 将已确诊的58例Hp阳性的RE病人随机分为两组.其中观察组30例,对照组28例.两组病人均给予口服质子泵抑制剂和对症治疗,观察组在治疗的同时加用克拉霉素和阿莫西林作Hp抗菌治疗,对照组则不作Hp抗菌治疗.总疗程均为4周.疗程结束后比较两组病人的症状、胃镜下黏膜损害的改善程度.结果 两组病人的症状改善程度差异无显著性,胃镜下黏膜损害改善程度差异亦无显著性.结论 清除Hp与否对于RE病人,无论是临床症状的控制,还是黏膜损害性病变的改善并不产生显著影响.
语种：
中文
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医学诊断学教学改革探讨
作者：
尹凯;王北冰;桂庆军;沈元琼;李熠
期刊：
高教论坛 ,2006年(6):81-82+119 ISSN：1671-9719
作者机构：
南华大学医学院诊断学教研室,湖南,衡阳,421001;[王北冰; 李熠; 沈元琼; 桂庆军; 尹凯] 南华大学
关键词：
诊断学;教学模式;循证医学
摘要：
诊断学在现代医学中的重要地位使其教学工作倍受关注,在医学教育理念和教学手段日新月异的情况下,传统的教学模式已不适应当前的教学形势,本文结合我校诊断学教研教改情况,对在新形势下如何合理利用资源,加强诊断学教学改革进行讨论。
语种：
中文
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姜黄素对血管平滑肌细胞源性荷脂细胞胆固醇代谢及SREBP-1表达的影响
作者：
匡双玉;庹勤慧;朱炳阳;梁磊;李熠;...
期刊：
中南医学科学杂志 ,2006年34(3):317-319,323 ISSN：2095-1116
作者机构：
南华大学,药物药理研究所,湖南,衡阳,421001;南华大学,医学院,诊断学教研室;[匡双玉; 庹勤慧; 李熠; 廖端芳; 罗迪贤; 梁磊; 朱炳阳; 许灿新] 南华大学
关键词：
姜黄素;荷脂细胞;胆固醇
摘要：
目的 观察姜黄素对血管平滑肌细胞源性荷脂细胞胆固醇代谢的影响,并初步探讨SREBP-1蛋白表达在其中的作用.方法 以血管平滑肌细胞源性荷脂细胞为模型,不同浓度姜黄素(12.5、25.0、50.0 μmol/L)作用于细胞24 h及25.0 μmol/L姜黄素不同时间(0、6、12、24、48 h)作用于细胞,高效液相色谱分析法检测细胞内总胆固醇.游离胆固醇和胆固醇酯的含量.在SREBP-1蛋白抑制剂ALLN干预下,25.0 μmol/L姜黄素处理平滑肌细胞源性荷脂细胞24 h,Western印迹法检测SREBP-1蛋白的表达.结果 不同浓度姜黄素(12.5、25.0、50.0 μmol/L)作用于血管平滑肌细胞源性荷脂细胞24 h及25.0 μmol/L姜黄素不同时间(0、6、12、24、48 h)作用于血管平滑肌细胞源性荷脂细胞,细胞内总胆固醇,游离胆固醇和胆固醇酯含量呈现一定的剂量和时间依赖性下降趋势; 在SREBP-1蛋白抑制剂ALLN干预下,25.0 μmol/L姜黄素处理平滑肌细胞源性荷脂细胞24 h,SREBP-1表达下调,总胆固醇、游离胆固醇和胆固醇酯含量无明显变化.结论 姜黄素能引起血管平滑肌细胞源性荷脂细胞总胆固醇、游离胆固醇和胆固醇酯含量下降,且这种作用跟SREBP-1蛋白的表达上调有一定关系.
语种：
中文
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Dynamic changes in orbitofrontal neuronal activity in rats during opiate administration and withdrawal
作者：
Sun, N;Li, Y;Tian, S;Lei, Y;Zheng, J;...
期刊：
Neuroscience ,2006年138(1):77-82 ISSN：0306-4522
通讯作者：
Lei, H
作者机构：
[Lei, H] Chinese Acad Sci, Kunming Inst Zool, Lab Primate Cognit Neurosci, Kunming 650223, Peoples R China.;Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 43007, Peoples R China.;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.;Chinese Acad Sci, Grad Sch, Hunan, Peoples R China.;Nanhua Univ, Med Sch, Hunan 412000, Peoples R China.
通讯机构：
[Lei, H] C;Chinese Acad Sci, Kunming Inst Zool, Lab Primate Cognit Neurosci, Kunming 650223, Peoples R China.
关键词：
Conditioned place preference;Drug craving;Gamma-band EEG;Manganese-enhanced MRI
摘要：
The orbitofrontal cortex is involved in the reinforcing effects of drugs of abuse. However, how the dynamic activity in OFC changes during opiate administration and withdrawal period has not been investigated. We first tested the effects of opiates and drug craving with the conditioned place preference paradigm, using manganese-enhanced magnetic resonance imaging and traditional electroencephalograph recording techniques in rats. T1-weighted 2D MRI (4.7 T) was used after unilateral injection of MnCl2 (200 nL, 80 mM) into the right orbitofrontal cortex. The manganese-enhanced magnetic resonance imaging data suggested that the OFC activity decreased during the opiate administration period but recovered increasingly during the withdrawal period. Also, we found decreases and increases in gamma-band (20-100 Hz) activity during the opiate administration and withdrawal period, respectively. Our results showed that orbitofrontal cortex activity decreased during morphine administration and then went up progressively over several days during withdrawal. The time course of the recovery of orbitofrontal activity from inhibition during the withdrawal period may be related to the experience of drug craving. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.
语种：
英文
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高血压病患者左心室质量与腰臀比的相关性
作者：
桂庆军;王北冰;沈元琼;陈扬勤;谢翠娥;...
作者机构：
[桂庆军; 王北冰; 沈元琼; 陈扬勤; 谢翠娥; 尹凯; 李熠; 梁丽红; 许丽芳; 徐刚] 南华大学医学院诊断学教研室;[桂庆军; 王北冰; 沈元琼; 陈扬勤; 谢翠娥; 尹凯; 李熠; 梁丽红; 许丽芳; 徐刚] 南华大学附属第一医院
会议名称：
第八次全国动脉硬化性疾病学术会议
会议时间：
2005-08
会议地点：
中国吉林延吉
关键词：
内科学;左心室质量指数;腰臀比;高血压病;收缩压
摘要：
<正> 目的研究高血压病患者及正常人左心室质量与腰臀比的相关性。方法测定118例高血压病患者、98例正常人超声下左心室质量指数、年龄、收缩压、舒张压、脉压、腰臀比及体质指数,进行成组t 检验、直线相关分析和多元逐步回归分析。结果高血压组收缩压、舒张压、脉压、左心室质量指数、腰臀比及体质指数均高于正常人组,差异有显著性。高血压组左心室质量指数与收缩压、舒张压、脉压和年龄呈正相关(r 分别为0.
语种：
中文
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