基于卓越医生教育培养计划的五年制临床医学人才培养模式的思考与实践
作者：
尹凯;谢娟;李熠;沈元琼;游咏;...
期刊：
医学教育研究与实践 ,2014年(3):442-443,496 ISSN：2096-3181
作者机构：
南华大学医学院诊断学教研室,湖南衡阳,421001;[李熠; 沈元琼; 桂庆军; 谢娟; 冯聚玲; 尹凯; 游咏] 南华大学
关键词：
卓越医生;五年制;临床医学;模式;思考
摘要：
我国卫生事业的发展和医药卫生体制改革对高等医学教育提出了更高要求,卓越医生教育培养计划是为提高医学人才培养质量而实施的改革举措之一。作者针对五年制临床医学人才卓越医生教育培养计划的内涵、目标、培养体系、培养方案和实践途径等方面进行探索和思考,为计划的实施提供了理论基础。
语种：
中文
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MicroRNA-27a/b regulates cellular cholesterol efflux, influx and esterification/hydrolysis in THP-1 macrophages
作者：
Zhang, Min;Wu, Jian-Feng;Chen, Wu-Jun;Tang, Shi-Lin;Mo, Zhong-Cheng;...
期刊：
Atherosclerosis ,2014年234(1):54-64 ISSN：0021-9150
通讯作者：
Tang, Chao-Ke
作者机构：
[Wu, Jian-Feng; Liu, Xiang-Yu; He, Ping-Ping; Ouyang, Xin-Ping; Chen, Wu-Jun; Peng, Juan; Zhang, Min; Li, Yuan; Lv, Yun-Cheng; Tang, Chao-Ke; Wang, Jia-Lin; Tang, Yan-Yan; Chen, Kong; Yao, Feng] Univ South China, Life Sci Res Ctr, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Wu, Jian-Feng; Tian, Guo-Ping] Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.;[Tang, Shi-Lin] Univ South China, Affiliated Hosp 1, Dept Intens Care Unit, Hengyang 421001, Hunan, Peoples R China.;[Wu, Jian-Feng; Tian, Guo-Ping] Univ South China, Dept Histol & Embryol, Hengyang 421001, Peoples R China.;[Tang, Deng-Pei] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7H 5E5, Canada.
通讯机构：
[Tang, Chao-Ke] U;Univ South China, Life Sci Res Ctr, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
关键词：
ABCA1;ACAT1;Atherosclerosis;CD36;Cholesterol homeostasis;LPL;apoA1;miR-27a/b
摘要：
Rationale: Macrophage cholesterol homeostasis maintenance is the result of a balance between influx, endogenous synthesis, esterification/hydrolysis and efflux. Excessive accumulation of cholesterol leads to foam cell formation, which is the major pathology of atherosclerosis. Previous studies have shown that miR-27 (miR-27a and miR-27b) may play a key role in the progression of atherosclerosis. Objective: We set out to investigate the molecular mechanisms of miR-27a/b in intracellular cholesterol homeostasis. Methods and results: In the present study, our results have shown that the miR-27 family is highly conserved during evolution, present in mammals and directly targets the 3' UTR of ABCA1, LPL, and ACAT1. apoA1, ABCG1 and SR-B1 lacking miR-27 bind sites should not be influenced by miR-27 directly. miR-27a and miR-27b directly regulated the expression of endogenous ABCA1 in different cells. Treatment with miR-27a and miR-27b mimics reduced apoA1-mediated cholesterol efflux by 33.08% and 44.61% in THP-1 cells, respectively. miR-27a/b also regulated HDL-mediated cholesterol efflux in THP-1 macrophages and affected the expression of apoA1 in HepG2 cells. However, miR-27a/b had no effect on total cellular cholesterol accumulation, but regulated the levels of cellular free cholesterol and cholesterol ester. We further found that miR-27a/b regulated the expression of LPL and CD36, and then affected the ability of THP-1 macrophages to uptake Dil-oxLDL. Finally, we identified that miR-27a/b regulated cholesterol ester formation by targeting ACAT1 in THP-1 macrophages. Conclusion: These findings indicate that miR-27a/b affects the efflux, influx, esterification and hydrolysis of cellular cholesterol by regulating the expression of ABCA1, apoA1, LPL, CD36 and ACAT1. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
语种：
英文
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脂联素减轻高糖诱导的人血管内皮细胞系损伤
作者：
李熠;匡双玉;尹凯;匡稳定;桂庆军
期刊：
基础医学与临床 ,2014年34(10):1386-1390 ISSN：1001-6325
作者机构：
[李熠; 尹凯; 桂庆军] 南华大学医学院诊断学教研室;[匡双玉; 匡稳定] 南华大学医学院附属第二医院
关键词：
D-葡萄糖;人脐静脉内皮细胞;脂联素
摘要：
目的观察脂联素(APN)对高糖诱导血管内皮细胞系损伤的保护作用及NF-κB与LOX-1蛋白表达在其中的作用。方法30 mmol/LD-葡萄糖作用于HUVEC-12(人脐静脉内皮细胞系),不同浓度的脂联素(10、20和40 μg/mL)作用48 h及20 μg/mL脂联素作用不同时间(12、24、48和72 h)后,倒置相差显微镜观察内皮细胞形态,Western blot检测核转录因子NF-KB和LOX-1蛋白表达,间接免疫荧光法检测NF-KB核转位情况。结果脂联素作用于D-葡萄糖诱导的HUVEC-12,内皮细胞形态改善,折光性增强,胞内颗粒物减少,细胞排列相对规整;同时,LOX-1蛋白表达下调(P <0. 05),NF-κB活性降低(P<0.05),并呈现时间和浓度依赖性(n =3,P <0. 05)。结论脂联素可能通过NF-KB 信号通路引起LOX-1蛋白表达下调,从而发挥其对高糖诱导血管内皮细胞损伤的保护作用。
语种：
中文
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Interleukin-27 inhibits foam cell formation by promoting macrophage ABCA1 expression through JAK2/STAT3 pathway
作者：
Fu, Hui;Tang, Yan-yan;Ouyang, Xin-ping;Tang, Shi-lin;Su, Hua;...
期刊：
Biochemical and Biophysical Research Communications ,2014年452(4):881-887 ISSN：0006-291X
通讯作者：
Tang, Chao-ke
作者机构：
[Huang, Li-ping; Fu, Hui; Su, Hua; Li, Xiaotao; Tang, Shi-lin; He, Miao] Univ South China, Affiliated Hosp 1, Dept ICU, Hengyang 421001, Hunan, Peoples R China.;[Lan, Gang; Zhang, Min; Liu, Dan; Li, Yuan; Tang, Chao-ke; Tan, Yu-lin; Lv, Yun-cheng; He, Ping-ping; Xie, Wei; Tang, Yan-yan; Ouyang, Xin-ping; Yao, Feng] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.;[Wu, Jianfeng; Chen, Kong; Zeng, Meng-ya] Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.;[Zheng, Xi-long] Univ Calgary, Dept Biochem & Mol Biol, Libin Cardiovasc Inst Alberta, Hlth Sci Ctr, Calgary, AB T2N 4N1, Canada.
通讯机构：
[Tang, Chao-ke] U;Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
ATP-binding cassette transporter A1;Foam cell;Interleukin-27;Janus kinase 2;Signal transducer and activator of transcription 3
摘要：
The purpose of this study is to determine whether IL-27 regulates macrophage ABCA1 expression, foam cell formation, and also explore the underlying mechanisms. Here, we revealed that IL-27 decreased lipid accumulation in THP-1 derived macrophages through markedly enhancing cholesterol efflux and increasing ABCA1 expression at both protein and mRNA levels. Our study further demonstrated that IL-27 increased ABCA1 level via activation of signal transducer and activator of transcription 3 (STAT3). Inhibition of Janus kinase 2, (JAK2)/STAT3 suppressed the stimulatory effects of IL-27 on ABCA1 expression. The present study concluded that IL-27 reduces lipid accumulation of foam cell by upregulating ABCA1 expression via JAK2/STAT3. Therefore, targeting IL-27 may offer a promising strategy to treat atherosclerotic vascular disease. © 2014 Elsevier Inc. All rights reserved.
语种：
英文
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《临床技能学》国家精品课程建设的研究与实践
作者：
李熠;沈元琼;桂庆军;文格波;游咏;...
期刊：
教育教学论坛 ,2013年(42):167-169 ISSN：1674-9324
作者机构：
南华大学 医学院,湖南 衡阳,421001;[格波; 李熠; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
临床技能学;国家精品课程;研究与实践
摘要：
国家精品课程建设对提高高校教育教学以及人才培养质量具有重要的作用。通过探索临床技能学培养新模式、推进立体化教学资源建设、改革创新教学方法和手段以及加强硬件环境的建设等措施，为提高国家级精品课程建设的质量和水平提供新的思路和方向。
语种：
中文
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2694株病原菌检出及药敏情况分析
作者：
李熠;匡双玉;桂庆军
期刊：
中国实用医药 ,2013年(31):162-162 ISSN：1673-7555
作者机构：
1. 湖南省衡阳南华大学医学院;2. 南华大学附属第二医院
关键词：
感染;病原菌;药敏;耐药
摘要：
目的了解某院患者感染的病原菌检出情况及相关药敏情况。方法使用上海复星佰路生物技术有限公司的BioFosun微生物鉴定及药敏分析系统进行菌种鉴定及药敏分析。结果检出菌株的标本总例数为3294例，检出的菌株共111种。各菌种耐药严重。结论患者感染细菌以阴性杆菌为主，呈现细菌种类多、耐药率高的特点，为科学合理使用抗生素提供实验依据。
语种：
中文
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3294株病原菌分布特征及四种细菌耐药情况分析
作者：
李熠;匡双玉;桂庆军
期刊：
中国实用医药 ,2013年8(30):167-168 ISSN：1673-7555
作者机构：
[李熠; 桂庆军] 南华大学医学院;[匡双玉] 南华大学附属第二医院
关键词：
病原菌;分布特征;耐药
摘要：
目的 了解某院患者感染的病原菌分布特征及相关的四种细菌耐药情况.方法 使用上海复星佰路生物技术有限公司的BioFosun微生物鉴定及药敏分析系统,对送检验科的样本进行菌种鉴定,同时对肺炎克雷伯菌、大肠埃希菌、金葡菌、肠球菌四种细菌进行耐药评价.结果 检出菌株的标本总例数为3294例,其中检出细菌的为2829例,占85.78％；检出真菌的为465例,占14.12％.肺炎克雷伯菌、大肠埃希菌、金葡菌、肠球菌四种细菌呈现不同程度的耐药.结论 病原菌分布以细菌为主,而细菌又以革兰氏阴性菌为主.检测的四种菌株耐药严重.
语种：
中文
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大临床技能教学模式在医学人才培养中的应用探讨
作者：
桂庆军;游咏;尹凯;李熠;沈元琼;...
期刊：
医学教育研究与实践 ,2013年21(3):460-461,468 ISSN：2096-3181
作者机构：
南华大学医学院诊断学教研室,湖南衡阳,421002;[李熠; 文格波; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
临床技能教学模式;医学人才;培养
摘要：
临床技能教学是临床医学教学中的核心内容之一，是高等医学教育的重要组成部分。建立贴近临床，贴近应用的科学、系统的大，临床技能教学模式，更符合现代医学教育要求，能确实提高医学生临床技能，使之能尽快适应现实医疗环境。符合现代医学模式下医学人才培养的改革思路。可为临床技能教学模式改革提供借鉴。
语种：
中文
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卓越医师医学人文素质教育的立体化教学模式的探讨
作者：
李熠;匡双玉;谢娟;游咏;桂庆军;...
期刊：
医学教育研究与实践 ,2013年21(6):1059-1060+1071 ISSN：2096-3181
作者机构：
南华大学医学院诊断学教研室,湖南衡阳,421001;南华大学附属第二医院,湖南衡阳,421001;[匡双玉] 南华大学第二附属医院;[李熠; 沈元琼; 桂庆军; 谢娟; 游咏] 南华大学
关键词：
卓越医师;人文素质教育;立体化教学
摘要：
通过构建立体化教学平台与教学管理体系,探索创新性的、立体化的教学方法和教学手段,开发和建设医学人文素质教育基地,为提高卓越医师医学人文素质教育教学效果、保证培养质量等提供了新的思路和帮助.
语种：
中文
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薯蓣皂苷元对人肝L-02细胞胆固醇代谢及Caveolin-1表达的影响
作者：
匡双玉;李熠;匡稳定
期刊：
中国药房 ,2013年24(35):3286-3288 ISSN：1001-0408
作者机构：
[匡双玉] 湖南中医药大学药学院;[匡稳定] 南华大学附属第二医院;[李熠] 南华大学医学院
关键词：
薯蓣皂苷元;肝细胞;胆固醇;胆固醇转运蛋白
摘要：
目的:研究薯蓣皂苷元对人肝L-02细胞胆固醇代谢的影响,并初步探讨胆固醇转运蛋白(Caveolin-1)在其中的表达变化.方法:正常人肝L-02细胞与50％胎牛血清共同孵育24h,以复制脂变肝细胞模型.不同质量浓度薯蓣皂苷元(0、20、40、80 μmol/L)作用于细胞24h与40μmo/L薯蓣皂苷元作用于细胞不同时间(0、6、12、24、48 h),油红O染色观察细胞内脂滴情况,高效液相色谱法检测细胞内总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)的含量,Western blot法检测Caveilin-1蛋白的表达.结果:0、20、40、80 μmol/L浓度下,薯蓣皂苷元可减少脂变人肝L-02细胞内TC、FC、CE含量,同时Caveolin-1蛋白表达上调,并呈现一定的剂量依赖性；在0、6、12、24、48 h时间点下,40 μmo/L薯蓣皂苷元可减少脂变人肝L-02细胞内TC、FC、CE含量,同时Caveolin-1蛋白表达上调,并呈现一定的时间依赖性.油红O染色,可见薯蓣皂苷作用下,脂变人肝-L02细胞内脂滴减少.结论:薯蓣皂苷元能降低脂变人肝L-02细胞胆固醇含量,同时上调Caveolin-1表达.
语种：
中文
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Retraction notice to “Myeloid-derived suppressor cells accumulate in the liver site after sepsis to induce immunosuppression” Cellular Immunology 279 (2012) 12–20
作者：
Ren, Dongping;Bi, Qi;Li, Li;Gao, Yongqiang;Liang, Yu;...
期刊：
Cellular Immunology ,2013年283(1-2):91 ISSN：0008-8749
通讯作者：
Xiao, Jianhua
作者机构：
[Liang, Yu; Li, Yingju; Li, Li; Xiao, Jianhua; Peng, Li; Gao, Yongqiang; Ren, Dongping; Liu, Jun] Univ South China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.;[Bi, Qi] Beijing Anzhen Hosp, Beijing 100092, Peoples R China.
通讯机构：
[Xiao, Jianhua] U;Univ South China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
摘要：
This article has been retracted: please see Elsevier Policy on Article Withdrawal.This article has been retracted at the request of the authors for the following reason: The results in Figure 4B are invalid because the conditions between the experimental group and the control group were not similar. The samples in the experimental group contained BSA whereas the samples in the control group did not. The authors found that BSA interferes with their experimental model and this makes the results invalid.
语种：
英文
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现代教育技术在医学生临床基本技能教学中的应用探讨
作者：
梁丽红;桂庆军;文格波;沈元琼;尹凯;...
期刊：
医学教育研究与实践 ,2013年21(3):561-563 ISSN：2096-3181
作者机构：
南华大学医学院,湖南衡阳,421001;[李熠; 唐志晗; 沈元琼; 文格波; 桂庆军; 尹凯; 游咏; 梁丽红] 南华大学
关键词：
现代教育技术;临床基本技能;立体化教学
摘要：
通过探讨基础仿真模拟技术、高级综合模拟人技术、多媒体教学技术、计算机辅助教学、网络教学等现代教育技术的特点,分析各种现代教育技术手段教学方法的优缺点,提出通过优化组合各技术手段方法,可以实现优势互补,相辅相成,从而建立临床基本技能的立体化教学模式,提高教学效果。
语种：
中文
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番茄红素对巨噬细胞源性荷脂细胞胆固醇代谢及其相关蛋白Srebp-1、Caveolin-1表达的影响
作者：
李熠;匡双玉;匡稳定
期刊：
中国药房 ,2013年24(21):1942-1944 ISSN：1001-0408
作者机构：
[李熠] 南华大学医学院;[匡双玉; 匡稳定] 南华大学附属第二医院;湖南中医药大学药学院
关键词：
番茄红素;荷脂细胞;胆固醇;固醇调节元件结合蛋白1;小凹蛋白1;表达
摘要：
目的:研究番茄红素对巨噬细胞源性荷脂细胞胆固醇代谢及其相关蛋白固醇调节元件结合蛋白1(Srebp-1)、小凹蛋白1(Caveolin-1)表达的影响。方法:建立巨噬细胞源性荷脂细胞模型,采用高效液相色谱法考察不同浓度(12.5、25、50 μmol/L)番茄红素作用于荷脂细胞24 h 及25 μmol/L番茄红素作用于荷脂细胞不同时间(12、24、48、72 h)后细胞内总胆固醇(TC)、游离胆固醇(FC)和胆固醇酯(CE)的含量;蛋白印迹法检测番茄红素(25 μmol/L、24 h)组及其与Srebp-1蛋白抑制剂ALLN(20 μmol/L、24 h)联用组对荷脂细胞中Srebp-1、Caveolin-1蛋白表达的影响。另设立正常巨噬细胞组和荷脂细胞组进行比较。结果:与正常巨噬细胞组比较,荷脂细胞组细胞内TC、FC、CE含量均明显升高,Srebp-1、Caveolin-1蛋白表达明显减弱(P<0.05);与荷脂细胞组比较,番茄红素组荷脂细胞内TC、FC、CE含量均明显降低且呈一定的浓度和时间依赖性,Srebp-1、Caveolin-1蛋白表达明显增强(P<0.05);与番茄红素组比较,联用组细胞内Srebp-1、Caveolin-1蛋白表达均明显减弱(P<0.05)。结论:番茄红素能引起巨噬细胞源性荷脂细胞内TC、FC、CE含量降低,且可能与Srebp-1及Caveolin-1蛋白的表达上调有一定关系。
语种：
中文
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MicroRNA Expression Abnormalities in Limited Cutaneous Scleroderma and Diffuse Cutaneous Scleroderma
作者：
Zhu, Honglin;Li, Yisha;Qu, Shunlin;Luo, Hui;Zhou, Yaou;...
期刊：
Journal of Clinical Immunology ,2012年32(3):514-522 ISSN：0271-9142
通讯作者：
Zuo, Xiaoxia
作者机构：
[You, Yunhui; Wang, Yanping; Zhu, Honglin; Li, Yisha; Luo, Hui; Zhou, Yaou; Zhao, Hongjun; Zuo, Xiaoxia] Cent S Univ, Dept Rheumatol, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Xiao, Xianzhong] Cent S Univ, Dept Pathophysiol, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China.;[Qu, Shunlin] Univ S China, Dept Pathophysiol, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Zuo, Xiaoxia] Cent S Univ, Dept Rheumatol, Xiangya Hosp, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Zuo, Xiaoxia] C;Cent S Univ, Dept Rheumatol, Xiangya Hosp, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
Scleroderma;microarray;microRNA;target gene
摘要：
Scleroderma (systemic sclerosis, SSc) is a complex autoimmune disease caused by progressive fibrotic replacement of normal tissue architecture, a progressive and ultimately fatal process that currently has no cure. Although dysregulation of microRNAs (miRNAs) is known to be involved in a variety of pathophysiologic processes, the role of miRNAs in SSc is unclear. In comparison with the normal skin tissues, miRNAs were aberrantly expressed in limited cutaneous scleroderma and diffuse cutaneous scleroderma skin tissues. We also identified miRNAs whose expressions were correlated with SSc fibrosis: miR-21, miR-31, miR-146, miR-503, miR-145, and miR-29b were predicted to be involved. This study further confirmed that miR-21 was increased whereas miR-145 and miR-29b were decreased both in the skin tissues and fibroblasts. As predicted target genes, SMAD7, SAMD3, and COL1A1 were regulated by these miRNAs. After stimulation with transforming growth factor β, the expression of miR-21 was increased and that of SMAD7 mRNA was decreased. MiR-145 was upregulated whereas the mRNA level of SMAD3 was downregulated. The downregulation of miR-29b was correlated with the upregulation of COL1A1 mRNA. MiRNAs might play an important role in the pathogenesis of SSc and suggest a potential therapy. © 2012 Springer Science+Business Media, LLC.
语种：
英文
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英国医学人才培养模式对我国医学教育改革的启示
作者：
莫明树;陈代娣;李熠;梁丽红;钟慧;...
期刊：
医学教育研究与实践 ,2012年20(6):1071-1072,1099 ISSN：2096-3181
作者机构：
南华大学医学院诊断学教研室,湖南衡阳,421001;南华大学附属第二医院神经外科,湖南衡阳,421001;[李熠; 冯聚铃; 莫明树; 钟慧; 梁丽红; 陈代娣] 南华大学;[王兵] 南华大学第二附属医院
关键词：
英国;医学教育;医患沟通;医学人文素质
摘要：
为了适应新世纪对高素质医学人才需求的挑战，我国高等医学院校在探索一条符合国情的医学人才培养道路，本研究通过收集和分析英国高等院校医学人才培养模式的相关资料与经验，与我国目前普遍实行的医学教育模式进行比较，分析其优缺点，以期为我国医学教育改革提供参考。
语种：
中文
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高等医学院校医德渗透教育的途径
作者：
李熠;桂庆军;唐志晗;陈代娣;尹凯;...
期刊：
医学教育研究与实践 ,2012年20(2):274-275 ISSN：2096-3181
作者机构：
南华大学医学院;[陈代娣] 南华大学船山学院;[李熠; 唐志晗; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
医德;渗透教育;途径
摘要：
医德教育是高等医学院校医学教育中的重要组成部分,医德渗透教育是培养医学生医德的重要渠道。对医学院校医德渗透教育的途径进行探讨,为提高医学院校医德教育的教学水平和教学效果提供了新的思路和方法。
语种：
中文
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Correlation between hammerhead ribozyme-mediated eggshell protein gene cleavage and reproduction inhibition of Schistosoma japonicum
作者：
Liang, Yu;Zhou, Yuelan;Yin, Weiguo;Li, Yingju;Yang, Qiulin;...
期刊：
MOLECULAR MEDICINE REPORTS ,2012年5(4):1087-1091 ISSN：1791-2997
通讯作者：
Xiao, Jianhua
作者机构：
[Liang, Yu; Gao, Yuan; Li, Yingju; Yin, Weiguo; Zhang, Yukuai; Xiao, Jianhua; Peng, Li; Yang, Qiulin; Zhou, Yuelan; Yang, Yaofei] Univ S China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.;[Li, Yingju] Univ S China, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, Jianhua] U;Univ S China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
关键词：
Schistosoma japonicum;eggshell protein gene;hammerhead ribozyme;in vitro cleavage;anti-reproduction
摘要：
Schistosoma japonicum (S. japonicum) is an extremely harmful pathogen, which infects humans and causes severe public health problems. To date, no effective therapeutic drugs for this pathogen are available. In this study, we designed and constructed three hammerhead ribozymes targeting the eggshell protein gene of S. japonicum (SjESG). The cleavage activities of these three ribozymes were determined using cleavage experiments. The in vitro cleavage results showed that among the three synthesized ribozymes (Rz1, Rz2 and Rz3), Rz1 and Rz3 cleaved their target RNAs effectively. However, Rz2 did not cleave its target RNA detectably. The putative therapeutic roles of these three ribozymes to inhibit the reproduction of S. japonicum in mice were studied in vivo. Compared with the negative controls, Rz1 and Rz3 treatments resulted in increased levels of IFN-γ but decreased levels of IL-4 in mice. Rz2 affected levels of IFN-γ and IL-4 to degrees similar with those caused by the vector controls. In addition, Rz1 and Rz3 reduced the amounts of adult worms and eggs in the livers of mice more extensively than Rz2 and the vector controls. Altogether, these results suggest a correlation between the in vitro cleavage abilities of Rz1 and Rz3 and their roles in reproduction inhibition of S. japonicum.
语种：
英文
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hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas
作者：
Wang, Yi;Luo, Hongmei;Li, Yangle;Chen, Tieding;Wu, Shengpeng;...
期刊：
MOLECULAR MEDICINE REPORTS ,2012年5(1):260-265 ISSN：1791-2997
通讯作者：
Yang, Luoyan
作者机构：
[Wang, Yi; Chen, Tieding; Yang, Luoyan; Wu, Shengpeng; Li, Yangle] Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.;[Luo, Hongmei] Univ S China, Dept Histol & Embryol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yang, Luoyan] C;Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
关键词：
hsa-miR-96;diagnostic marker;MAP4K1;insulin receptor substrate 1
摘要：
Numerous microRNAs (miRNAs) play crucial roles in cancer development. In this study, we report that hsa-miR-96 is expressed at higher levels in human bladder urothelial carcinomas compared to normal tissues. We found that hsa-miR-96 increased invasion and differentiation of human bladder T24 cells and promoted their growth. Downregulation of hsa-miR-96 significantly affected the phenotype of bladder cancer T24 cells. The mRNA and protein levels of insulin receptor substrate 1 (IRS1) and MAP4K1 were significantly reduced in cells transfected with the hsa-miR-96 inhibitor when compared with levels in cells transfected with the empty plasmid vector or the negative control miRNA inhibitor. Altogether, these results suggest that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating IRS1 and MAP4K1 levels, functioning as a promising diagnostic marker in human bladder urothelial carcinomas.
语种：
英文
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血清ICAM-1浓度与冠脉侧支循环形成与否的关系
作者：
游咏;莫靓;桂庆军;尹凯;李熠;...
期刊：
吉林医学 ,2012年33(16):3367-3368 ISSN：1004-0412
作者机构：
南华大学医学院诊断学教研室,衡阳,421001;[李熠; 莫靓; 沈元琼; 桂庆军; 尹凯; 游咏] 南华大学
关键词：
冠状动脉;侧支循环;非对称性二甲基精氨酸
摘要：
目的:检测冠脉严重狭窄的冠心病患者的血清ICAM-1浓度,探讨ICAM-1 浓度与冠脉侧支循环形成与否的相关性.方法:通过冠脉造影检查,收集主要冠脉至少一支狭窄程度在95%以上的108例冠心病患者,测定其血清ICAM-1浓度.采用Rentrop方法对冠脉侧支循环进行分级,0级为侧支循环未形成组(n=47),1～3级为侧支循环形成组(n=61).结果:与侧支循环形成组(243.08±70.90 ng/ml)相比,侧支循环未形成组血清ICAM-1浓度(286.38±56.27 ng/ml)明显增高,差异有统计学意义(P=0.001,P＜0.01).结论:冠脉严重狭窄的冠心病患者血清ICAM-1浓度与冠脉侧支循环形成呈负相关.
语种：
中文
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Myeloid-derived suppressor cells accumulate in the liver site after sepsis to induce immunosuppression.
作者：
Ren, Dongping;Bi, Qi;Li, Li;Gao, Yongqiang;Liang, Yu;...
期刊：
Cellular immunology ,2012年279(1):12-20 ISSN：0008-8749
通讯作者：
Xiao, J.
作者机构：
[Ren, Dongping] Institute of Pathogenic Biology, University of South China, Hunan 421001, PR China
通讯机构：
Institute of Pathogenic Biology, University of South China, China
关键词：
CLP;Immune suppression;Myeloid-derived suppressor cells;Sepsis
摘要：
Myeloid-derived suppressor cells (MDSCs) play a major role in modulating immune response, but only a few reports focused on MDSCs in the liver of sepsis states. Here, we investigated the changes in MDSCs in liver of the cecal ligation and puncture (CLP) mice. The results of flow cytometry showed that MDSCs accumulate in the liver site of mice after CLP for 7. days (CLP7d model mice) and settled to the livers of both normal and LPS stimulated mice. In vitro experiment showed a strong suppressive effect of MDSCs on the proliferation of CD4+ T lymphocytes of spleen. Furthermore, adoptive transfer of the liver MDSCs from CLP7d miceincreased the survival rate of acute hepatic failure (AHF) model mice in vivo. In conclusion, our data suggest that sepsis-induced liver MDSCs may have exert a key role in maintaining the immune homoeostasis in liver during the sepsis state. © 2012 Elsevier Inc.
语种：
英文
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