Expression and Purification of Tag-removed Human IL37 by Digestion on Beads in Escherichia coli

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成果类型：

期刊论文

作者：

Wu, Daichao;Li, Guoqing;Ma, Yao;Liu, Jin;Li, Yukun;...

通讯作者：

Chen, Yongheng;Tan, Sijie;Li, Meixiang

作者机构：

[Li, Yukun; Tan, Sijie; Bai, Junhui; Liu, Jin; Li, Meixiang; Wu, Daichao; Wang, Juan] Univ South China, Hengyang Med Coll, Inst Clin Anat & Reprod Med, Dept Histol & Embryol, Hengyang 421001, Hunan, Peoples R China.

[Wu, Daichao; Li, Guoqing] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.

[Ma, Yao] Univ South China, Network Informat Ctr, Hengyang 421001, Hunan, Peoples R China.

[Chen, Yongheng] Cent S Univ, XiangYa Hosp, Chinese Minist Hlth, Lab Struct Biol,Key Lab Canc Prote, Changsha 410008, Hunan, Peoples R China.

[Chen, Yongheng] Cent S Univ, XiangYa Hosp, Changsha 410008, Hunan, Peoples R China.

通讯机构：

[Chen, Yongheng] C

[Tan, SJ; Li, MX] U

Cent S Univ, XiangYa Hosp, Changsha 410008, Hunan, Peoples R China.

Univ South China, Hengyang Med Coll, Hengyang 421000, Peoples R China.

语种：

英文

关键词：

Soluble expression;purification;interleukin 37;Escherichia coli;digestion on beads;inflammatory diseases.

期刊：

PROTEIN AND PEPTIDE LETTERS

ISSN：

0929-8665

年：

2018

卷：

期：

页码：

996-1002

DOI：

10.2174/0929866526666181128124028

基金类别：

Scientific and Technological Advance Project of Hengyang City [2017KJ303]; Key Lab for Clinical Anatomy & Reproductive Medicine of Hengyang City [2017KJ182]; Undergraduate Research and Innovation Experimental Program of the University of South China [201XJXZ161]

机构署名：

本校为第一且通讯机构

院系归属：

医学院

药学与生物科学学院

摘要：

Background: Human Interleukin 37 (IL37), a unique anti-inflammatory cytokine of IL1 family member, plays critical roles in innate and adaptive immunity and inflammation. Objective: Preparation of high purity and tag-removed recombinant IL37 protein (rIL37) is critical for its clinical application. Method: In this study, we constructed an N-terminal cleavable GST-fused IL37 expression vector for recombinant expression. Results: Subsequent to transformation and optimization of the induction temperature, the soluble expression level of rIL37 was 3...

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Expression and Purification of Tag-removed Human IL37 by Digestion on Beads in Escherichia coli

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