作者：
Ye, Qiong;Tian, Guo-Ping;Cheng, Hai-Peng;Zhang, Xin;Ou, Xiang;...
期刊：
Journal of Atherosclerosis and Thrombosis,2018年25(3):244-253 ISSN：1340-3478
通讯作者：
Tang, Chao-Ke
作者机构：
[Zhang, Xin; Gong, Duo; Cheng, Hai-Peng; Ye, Qiong; Xia, Xiao-Dan; Zhang, Min; Tang, Chao-Ke; Zhao, Zhen-Wang; Yu, Xiao-Hua; Li, Liang; Xie, Wei; Huang, Chong; Chen, Ling-Yan] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Med Res Ctr, Inst Cardiovasc Res,Key Lab Atherosclerol Hunan P, Hengyang, Hunan, Peoples R China.;[Tan, Ru-Qi; Ye, Qiong; Tian, Guo-Ping; Yang, Feng-Yun; Pan, Yan-Jun] Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang, Hunan, Peoples R China.;[Ou, Xiang] First Hosp Changsha, Dept Endocrinol, Changsha, Hunan, Peoples R China.;[Zhang, Jie] Univ South China, Affiliated Hosp 2, Dept Spinal Surg, Hengyang, Hunan, Peoples R China.;[Zheng, Xi-Long] Univ Calgary, Hlth Sci Ctr, Libin Cardiovasc Inst Alberta, Cumming Sch Med,Dept Biochem & Mol Biol, 3330 Hosp Dr NW, Calgary, AB, Canada.
通讯机构：
[Tang, Chao-Ke] U;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
MiR-134;ANGPTL4;LPL;Atherosclerosis
摘要：
Aims: Atherosclerosis is the most common cause of cardiovascular disease, such as myocardial infarction and stroke. Previous study revealed that microRNA (miR)-134 promotes lipid accumulation and proinflammatory cytokine secretion through angiopoietin-like 4 (ANGPTL4)/lipid lipoprotein (LPL) signaling in THP-1 macrophages. Methods: ApoE KO male mice on a C57BL/6 background were fed a high-fat/high-cholesterol Western diet, from 8 to 16 weeks of age. Mice were divided into four groups, and received a tail vein injection of miR-134 agomir, miR-134 antagomir, or one of the corresponding controls, respectively, once every 2 weeks after starting the Western diet. After 8 weeks we measured aortic atherosclerosis, LPL Activity, mRNA and protein levels of ANGPTL4 and LPL, LPL/low-density lipoprotein receptor related protein 1 Complex Formation, proinflammatory cytokine secretion and lipid levels. Results: Despite this finding, the influence of miR-134 on atherosclerosis in vivo remains to be determined. Using the well-characterized mouse atherosclerosis model of apolipoprotein E knockout, we found that systemic delivery of miR-134 agomir markedly enhanced the atherosclerotic lesion size, together with a significant increase in proinflammatory cytokine secretion and peritoneal macrophages lipid contents. Moreover, overexpression of miR-134 decreased ANGPTL4 expression but increased LPL expression and activity in both aortic tissues and peritoneal macrophages, which was accompanied by increased formation of LPL/low-density lipoprotein receptor-related protein 1 complexes in peritoneal macrophages. However, an opposite effect was observed in response to miR-134 antagomir. Conclusions: These findings suggest that miR-134 accelerates atherogenesis by promoting lipid accumulation and proinflammatory cytokine secretion via the ANGPTL4/LPL pathway. Therefore, targeting miR-134 may offer a promising strategy for the prevention and treatment of atherosclerotic cardiovascular disease.
摘要：
A novel turn-on fluorescent probe based on trimethyl lock with acrylate recognition moiety and latent fluorophore of 4-amino-7-nitrobenz-2-oxa-1,3-diazole (NBD-NH2) for the detection of glutathione has been developed. The probe can selectively and sensitively detect glutathione in solution, and the limit of detection was calculated to be 26 nM. Furthermore, the probe has been successfully used to detect GSH in living cells. The interesting preference of GSH was attributed to the steric repulsion of trimethyl which retarded Cys, Hcy to reach Burgi-Dunitz angle and allowed more flexible side chain of GSH to attack carbonyl functionality. (C) 2017 Elsevier B.V. All rights reserved.
通讯机构：
[Zheng, Xing] U;[Zhao, Weili] F;[Zheng, Xing] H;Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China.
关键词：
fluorescent probe;palladium;living cell
摘要：
A novel turn-on fluorescent probe for the detection of palladium has been designed. The probe can selectively and sensitively detect palladium in solution, and the limit of detection was calculated to be 11.4 nmol?L~(-1). Furthermore, the probe was successfully used for fluorescence imaging of palladium in living cells.
作者：
Jin Zhang;Guang-ya Zhang;Jin Xu;Wen-bo Wu;Xin-yang Sun;...
期刊：
African Journal of Psychiatry,2015年18(2):1-6 ISSN：1994-8220
通讯作者：
Zhang, L.-Y.
作者机构：
[Zhang J.] School of medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China;[Zhang G.-Y.] Department of Psychiatry, Suzhou Psychiatric Hospital, Suzhou, Jiangsu, 215101, China;[Xu J.] Administration office, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, 213003, China;[Wu W.-B.] Department of outpatients, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, 213003, China;[Sun X.-Y.; Zhang L.-Y.] Prevention and Treatment Center for Psychological Diseases, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, 213003, China
通讯机构：
[Zhang, L.-Y.] P;Prevention and Treatment Center for Psychological Diseases, No.102 hospital of Chinese People's Liberation Army, North Peace Road 55, Changzhou, Jiangsu, China
关键词：
Jin Zhang;Guang-ya Zhang;Jin Xu;Wen-bo Wu;Xin-yang Sun;Li-yi Zhang and Shu-ya He;Schizophrenia;miRNA-7;Target genes;Interaction
作者机构：
[Li, Kai; Sun, Aijuan; Zhang, Jia; Wang, Qinglin; Pan, Yunzhi] Laboratory of Molecular Medicine, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China;Suzhou Institute of Chinese Materia Medica, Suzhou 215123, China;Laboratory of Molecular Medicine, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China;[Zhou, Cuilan] Department of Pharmacology, Nanhua University, Hengyang, Hunan 421001, China;[Yi, Chun] College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China
通讯机构：
[Li, K.] L;Laboratory of Molecular Medicine, College of Pharmaceutical Science, Soochow University, China
摘要：
We propose a short definition of GENOME: The full complement of genetic materials possessed by an intracellular parasite, a cell, or an organism. Accordingly, the human genome is the entire complement of inherited genetic materials possessed by an individual person, or possessed by a cell in an individual person. For higher species, the genomic makeup includes DNA in the nucleus and in the organelles regardless of the number of chromosomes and the homoplasmic or heteroplasmic status of the mitochondrial or chloroplastic DNA. Practically, GENOME can be referred to at the molecular, cellular, individual, and species levels, which has various implications in biotechnological research and molecular diagnostics.
作者机构：
[Xiao, L.; Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215006, Peoples R China.;[Xiao, L.; Li, Kai] Suzhou Univ, Second Affilated Hosp, Suzhou 215006, Peoples R China.;[Xiao, L.; Zhang, J.; Chen, C. L.; Li, Kai] Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.;[Zhang, J.] Novartis Res Fdn, Genom Inst, San Diego, CA USA.;[Yin, Y. F.] City Hope Natl Med Ctr, Duarte, CA 91010 USA.
通讯机构：
[Zhang, I ] ;Nanhua Univ, SNP Inst, Hengyang, Hunan, Peoples R China.
期刊：
Biochemical and Biophysical Research Communications,2005年328(1):265-272 ISSN：0006-291X
通讯作者：
Liao, DF
作者机构：
[Liao, DF] Nanhua Univ, SNP Inst, Inst Pharm & Pharmacol, Hengyang, Hunan, Peoples R China.;Ctr S Univ, Dept Pharmacol, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;Natl Nat Sci Fdn China, Div Cell Biol Genet & Dev Biol, Dept Life Sci, Beijing 100085, Peoples R China.;Ctr S Univ, Canc Res Inst, Changsha 410078, Hunan, Peoples R China.;City Hope Natl Med Ctr, Duarte, CA 91010 USA.
通讯机构：
[Liao, DF] N;Nanhua Univ, SNP Inst, Inst Pharm & Pharmacol, Hengyang, Hunan, Peoples R China.
期刊：
Trends in Biotechnology,2005年23(2):92-96 ISSN：0167-7799
通讯作者：
Li, K
作者机构：
[Li, K] Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.;Geomapping Inc, Tianjin, Peoples R China.;City Hope Natl Med Ctr, Dept Human Genet, Duarte, CA 91010 USA.;First Mil Med Univ, Inst Oncol, Guangzhou 510515, Peoples R China.
通讯机构：
[Li, K] N;Nanhua Univ, N Dist Sch, SNP Inst, Hengyang 421001, Hunan, Peoples R China.
摘要：
DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3' dehydroxylation modifications. Accordingly, three new types of single nucleotide polymorphism (SNP) assays have been developed to carry out genome-wide genotyping making use of the fidelity advantage of exo(+) polymerases. Such SNP assays might also provide a novel approach for re-sequencing and de novo sequencing. These new mutation detection assays are widely adaptable to a variety of platforms, including real-time PCR, multi-well plate and microarray technologies. Application of exo(+) polymerases to genetic analysis could accelerate the pace of personalized medicine.
关键词：
random peptide phage library;vascular endothelial cell;whole-cell screening;cell adhesion;caveolin-1;eNOS
摘要：
Using oxidized low-density lipoprotein (LDL)-injured vascular endothelial cells (ECs) as target cells, peptides specifically binding to the injured ECs were screened from a phage-displaying peptide library by using the whole-cell screening technique after three cycles of the “adsorption-elution-amplification” procedure. Positive phage clones were identified by ELISA, and the inserted amino acid sequences in the displaying peptides were deduced from confirmation with DNA sequencing. The adhesion rate of ECs to monocytes was evaluated by cell counting. The activity of endothelial nitric oxide synthase (eNOS), and the expression levels of caveolin-1 and intercellular adhesion molecule-1 (ICAM-1) were determined by Western blotting. Six positive clones specifically binding to injured ECV304 endothelial cells were selected from fourteen clones. Interestingly, four phages had peptides with tandem leucine, and two of these even shared an identical sequence. Functional analysis demonstrated that the YCPRYVRRKLENELLVL peptide shared by two clones inhibited the expression of ICAM-1, increased nitric oxide concentration in the culture media, and upregulated the expression of caveolin-1 and eNOS. As a result, the adhesion rate of monocytes to ECV304 cells was significantly reduced by 12.1%. These data suggest that the anti-adhesion effect of these novel peptides is related to the regulation of the caveolin-1/nitric oxide signal transduction pathway, and could be of use in potential therapeutic agents against certain cardiovascular diseases initiated by vascular endothelial cell damage.
作者机构：
City Hope Natl Med Ctr, Duarte, CA 91010 USA.;Nanhua Univ, SNP Inst, Hengyang, Peoples R China.;Genomapping Inc, Tianjin, Peoples R China.;[Li, K] City Hope Natl Med Ctr, 1500 E Duarte Rd, Duarte, CA 91010 USA.
通讯机构：
[Li, K] C;City Hope Natl Med Ctr, 1500 E Duarte Rd, Duarte, CA 91010 USA.
摘要：
The role of 3' exonuclease excision in DNA polymerization was evaluated for primer extension using inert allele specific primers with exonuclease-digestible ddNMP at their 3' termini. Efficient primer extension was observed in amplicons where the inert allele specific primers and their corresponding templates were mismatched. However, no primer-extended products were yielded by matched amplicons with inert primers. As a control, polymerase without proofreading activity failed to yield primer-extended products from inert primers regardless of whether the primers and templates were matched or mismatched. These data indicated that activation was undertaken for the inert allele specific primers through mismatch proofreading. Complementary to our previously developed SNP-operated on/off switch, in which DNA polymerization only occurs in matched amplicon, this new mutation detection assay mediated by exo(+) DNA polymerases has immediate applications in SNP analysis independently or in combination of the two assays.
