期刊论文

Induction Mechanism of ppr Gene Deletion Triggering Flocculation in Schizosaccharomyces pombe

中文

微生物学杂志

1005-7021

2019

39

2

11-17

10.3969/j.issn.1005-7021.2019.02.002

国家自然科学基金青年科学基金项目(31400032) 江苏省高校自然科学研究项目(13KJB180010).

本校为第一机构

粟酒裂殖酵母中PPR蛋白在线粒体和叶绿体的RNA代谢中起重要作用, ppr基因的缺失会引起线粒体功能异常(ppr5除外)。旨在揭示粟酒裂殖酵母中ppr 基因缺失引起细胞絮凝的机制。利用含有半乳糖的培养基分别培养WT、Δppr3、Δppr4、Δppr6和Δppr10,观察野生型和突变体的生长表型;通过添加不同的金属离子及改变细胞表面 pH来探究诱导这类突变体出现絮凝的因素;采用RT-PCR技术检测缺陷菌中编码细胞表面絮凝因子及细胞壁重构酶基因的表达情况。研究结果表明: ppr3、ppr4、ppr6和ppr10的敲除会引起由 Ca^2+依赖型半乳糖结合蛋白参与的细胞絮凝;不同的pH和不同金属离子对这些缺陷菌的细胞絮凝有影响;ppr...

Pentatricopeptide repeat (PPR) proteins plays important role in mitochondrial and chloroplast in Schizosaccharomyces pombe RNA metabolism, the deletion of ppr gene will cause functional anomaly of mitochondria (expect for ppr5 ). The aim of this paper is to reveal the mechanism of ppr gene deletion that triggering cell flocculation. Disruption of ppr gene result in mitochondrial dysfunction(except ppr 5). This paper aims to reveal the mechanism of cell flocculation caused by loss of ppr gene in Schizosaccharomyces pombe . The growth phenotypes ...