NIR-II light evokes DNA cross-linking for chemotherapy and immunogenic cell death
作者：
Huang, Yun;Wei, Dengshuai;Wang, Bin;Tang, Dongsheng;Cheng, Ailan;...
期刊：
Acta Biomaterialia ,2023年160:198-210 ISSN：1742-7061
通讯作者：
Yu, Yingjie(yuyingjie@mail.buct.edu.cn)
作者机构：
[Huang, Yun; Huang, Weiguo; Xiao, Shengjun] Guilin Med Univ, Guangxi Key Lab Tumor Immun & Microenvironm Regula, Guilin 541199, Peoples R China.;[Cheng, Ailan; Huang, Yun; Huang, Weiguo] Univ South China, Canc Res Inst, Hengyang Med Coll, Hengyang 421001, Peoples R China.;[Wei, Dengshuai; Tang, Dongsheng; Wang, Bin] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China.;[Yu, Yingjie] Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China.
通讯机构：
[Yingjie Yu] S;[Weiguo Huang] G;State Key Laboratory of Organic-Inorganic Composites;Beijing Laboratory of Biomedical Materials;Beijing University of Chemical Technology, Beijing 100029, China<&wdkj&>Guangxi Key Laboratory of Tumor Immunity and Microenvironment Regulation, Guilin Medical University, Guilin 541199, China<&wdkj&>Cancer Research Institute, Hengyang Medical College of University of South China, Hengyang 421001, China
关键词：
Borates;Cell Line, Tumor;DNA;Humans;Immunogenic Cell Death;Immunotherapy;Nanoparticles;Neoplasms;Oxaliplatin;Chemotherapy;Diseases;DNA;Immune system;Infrared devices;Irradiation;Nanoparticles;Platinum compounds;Tumors;gamma interferon;ir 1061;nanoparticle;oxaliplatin;photosensitizing agent;polymer;reactive oxygen metabolite;tumor necrosis factor;unclassified drug;boric acid;DNA;IR1061;oxaliplatin;Cancer immunotherapy;Cross linking;DNA damages;DNA damaging agents;Immune response;Immunogenic cell death;Immunotherapy;Mild hyperthermia;Oxaliplatin;Therapeutic outcomes;4T1 cell line;animal cell;animal experiment;animal model;animal tissue;antineoplastic activity;apoptosis;Article;cancer chemotherapy;CD4+ T lymphocyte;CD8+ T lymphocyte;controlled study;cross presentation;cytokine release;DNA cross linking;DNA damage;drug efficacy;drug release;female;human;immune response;immunogenic cell death;in vitro study;in vivo study;mouse;nanoencapsulation;near-infrared photoimmunotherapy;nonhuman;thermotherapy;triple negative breast cancer;immunotherapy;neoplasm;tumor cell line;Cell death
摘要：
As a DNA damaging agent, oxaliplatin (OXA) can induce immunogenic cell death (ICD) in tumors to activate the immune system. However, the DNA damage induced by OXA is limited and the ICD effect is not strong enough to enhance anti-tumor efficacy. Here, we propose a strategy to maximize the ICD effect of OXA through the mild hyperthermia generated by nanoparticles with a platinum (IV) prodrug of OXA (Pt(IV)-C16) and a near-infrared-II (NIR-II) photothermal agent IR1061 upon the irradiation of NIR-II light. The mild hyperthermia (43 °C) holds advantages in two aspects: 1) increase the Pt-DNA cross-linking, leading to enhanced DNA damage and apoptosis; 2) induce stronger ICD effects for cancer immunotherapy. We demonstrated that, compared with OXA and photothermal therapy of IR1061 alone, these nanoparticles under NIR-II light irradiation can significantly improve the anti-cancer efficacy against triple-negative breast cancer 4T1 tumor. This new strategy provides an effective way to improve the therapeutic outcome of OXA. Statement of significance: OXA could induce immunogenic cell death (ICD) via stimulating immune responses by increasing tumor cell stress and death, which triggers tumor-specific immune responses to achieve immunotherapy. However, due to the insufficient Pt-DNA crosslinks, the ICD effect triggered by OXA cannot induce robust immune response. Mild hyperthermia has great potential to maximize the therapeutic outcome of oxaliplatin by increasing the Pt-DNA cross-linking to augment the immunoresponse for enhanced cancer immunotherapy. © 2023 Acta Materialia Inc.
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Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform
作者：
Chen, Yulin;Gao, Dan;Zhu, Qingyun;Chu, Bizhu;Peng, Jie;...
期刊：
ANALYST ,2023年148(10):2387-2394 ISSN：0003-2654
通讯作者：
Gao, Dan(gaogao.dan@sz.tsinghua.edu.cn)
作者机构：
[Peng, Jie; Jiang, Yuyang; Gao, Dan; Chen, Yulin] Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, State Key Lab Chem Oncogen, Guangdong Prov Key Lab Chem Biol, Shenzhen 518055, Guangdong, Peoples R China.;[Zhu, Qingyun] Univ South China, Affiliated Hosp 1, Canc Res Inst, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Chu, Bizhu] Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Shenzhen 518060, Peoples R China.;[Wang, Jian] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Thorac Surg, Shenzhen 518020, Guangdong, Peoples R China.;[Wang, Jian; Liu, Liping] Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China.
通讯机构：
[Dan Gao] S;D. Gao;State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, 518055, China;email: gaogao.dan@sz.tsinghua.edu.cn
关键词：
Exosomes;Humans;Membrane Proteins;Microfluidics;MicroRNAs;Neoplasms;Oligonucleotides;Cancer cells;Diagnosis;Diseases;Fluorescence;Microfluidics;Proteins;RNA;membrane protein;microRNA;oligonucleotide;Aptamers;Cancer diagnosis;Cancer prognosis;Diagnosis and prognosis;Early cancer;Exosomes;Microfluidic platforms;Multiplexed detection;Research studies;Surface proteins;chemistry;exosome;human;metabolism;microfluidics;neoplasm;Biomarkers
摘要：
Exosomes are considered as promising biomarkers for early cancer diagnosis and prognosis. However, the majority of the research studies focused on a single type of exosomal biomarkers, which cannot comprehensively reflect the state of cancer for accurate diagnosis. To address this problem, we presented a ship-shaped microfluidic device containing a microcolumn array for simultaneous in situ detection of exosomal surface proteins and miRNAs. Exosomes were first captured in the microchannels modified with CD63 protein aptamer. Exosomal surface proteins and miRNAs were simultaneously detected in four parallel channels to avoid the interference of fluorescent signals using specific aptamers labeled by Cy5 and catalytic hairpin assembly (CHA) based signal amplification strategy. The limit of detection for multiplexed markers in exosomes was 83 exosomes per μL, which is comparable to previously reported methods. Through quantitative analysis of two disease-specific surface proteins and miRNAs derived from different cancer cells and clinical serum samples, different cancer subtypes as well as cancer patients and healthy people could be significantly distinguished. These results suggest that this simple, highly sensitive, and more accurate analytical strategy by simultaneous in situ profiling of different types of exosomal biomarkers has potential applications in cancer diagnosis and stage monitoring. © 2023 The Royal Society of Chemistry.
语种：
英文
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Rational design of molecular phototheranostic platform for NIR-II fluorescence imaging guided chemodynamic-photothermal combined therapy
作者：
Wu, Gui-long;Sun, Bin;He, Yuxuan;Tan, Xiaofeng;Pan, Qi;...
期刊：
Chemical Engineering Journal ,2023年463:142372 ISSN：1385-8947
通讯作者：
Tang, Li(tanglicq@outlook.com)
作者机构：
[Xiao, Hao; Tang, Li; Li, Na; Liu, Fen; Tan, Xiaofeng; Wang, Minghui; Pan, Qi; Yang, Qinglai; Wu, Gui-long; He, Yuxuan; Wu, Peixian; Yang, Sha] Univ South China, Canc Res Inst, Ctr Mol Imaging Probe, Hengyang Med Sch,Hunan Prov Key Lab Tumor Cellular, Hengyang 421001, Hunan, Peoples R China.;[Zhu, Shoujun; Sun, Bin] Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China.;[Tang, Li] Hainan Normal Univ, Coll Chem & Chem Engn, Key Lab Trop Med Plant Chem, Minist Educ, Haikou 571158, Hainan, Peoples R China.
通讯机构：
[Li Tang; Qinglai Yang] C;[Shoujun Zhu] S;Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China<&wdkj&>State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China<&wdkj&>Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China<&wdkj&>Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, Hainan 571158, China
关键词：
Hyperthermia therapy;Infrared devices;Infrared lasers;Irradiation;Peptides;Tumors;Chemodynamic-photothermal combined therapy;Chemodynamics;Combined therapy;Disintegrable nanoassembly;Fluorescence imaging;Glutathione depletion;Gluthathione;Immunity effect;Nano-assemblies;NIR-II fluorescence imaging;Photo-thermal;Fluorescence imaging
摘要：
The strategy of synergetic chemodynamic treatment (CDT) and photothermal therapy (PTT) to improve the anticancer effect has been a hot spot in the field of biomedicine. Herein, a high-performance near-infrared II (NIR-II) fluorescence imaging-guided molecular phototheranostic platform (IR-FE-Fc@DSPE-S-S-PEG) was designed via a convenient strategy. IR-FE-Fc@DSPE-S-S-PEG can serve as a PTT agent to kill cancer cells under near-infrared laser (808 nm) irradiation and a CDT agent to convert the endogenously less reactive H2O2 into the harmful •OH, simultaneously deplete the glutathione (GSH) to achieve an amplified CDT as well. Notably, PTT-induced hyperthermia can further enhance the CDT effect, achieving a synergistic PTT/CDT therapy. Due to the enhanced permeability and retention (EPR) effect and the high GSH microenvironment of the tumor, IR-FE-Fc@DSPE-S-S-PEG exhibited much higher tumor accumulation revealed by NIR-II fluorescence imaging. More importantly, IR-FE-Fc@DSPE-S-S-PEG efficiently damages tumorous tissues without inducing side effects to normal tissues under 808 nm laser irradiation. This work has proposed an alternative PTT-CDT combined therapy platform based on a rational design of disintegrable NIR-II fluorescence imaging-guided molecular for effective tumor treatment. © 2023 Elsevier B.V.
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英文
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Development of a Golgi-targeted superoxide anion fluorescent probe for elucidating protein GOLPH3 function in myocardial ischemia-reperfusion injury
作者：
Li, Ao;Liu, Yalan;Chen, Zhe;Li, Songjiao;Zhong, Rongbin;...
期刊：
Analytica Chimica Acta ,2023年1255:341100 ISSN：0003-2670
通讯作者：
He, Longwei(helongwei0110@163.com);Chen, Linxi(1995001765@usc.edu.cn)
作者机构：
[Li, Ao; He, Longwei; Liu, Yalan; Chen, Zhe; Li, Songjiao; Chen, Linxi] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharm, Hengyang Med Sch,Hunan Prov Key Lab Tumor Microen, Hengyang 421001, Peoples R China.;[Zhong, Rongbin; Cheng, Dan] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hengyang Med Sch, Hengyang 421002, Peoples R China.
通讯机构：
[Linxi Chen; Longwei He] S;L. He;School of Pharmacy, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang Medical School, University of South China, Hengyang, 421001, China;email: helongwei0110@163.com;L. Chen
关键词：
Animals;Fluorescent Dyes;Golgi Apparatus;Myocardial Reperfusion Injury;Oxygen;Reactive Oxygen Species;Superoxides;Zebrafish;Diagnosis;Fluorescence imaging;Fluorescence spectroscopy;Ions;Probes;Proteins;fluorescent dye;golgi phosphoprotein 3;phosphoprotein;reactive oxygen metabolite;superoxide;unclassified drug;fluorescent dye;oxygen;reactive oxygen metabolite;Cell apoptosis;Fluorescent probes;Golgi apparatus;Golgi phosphoprotein 3;Ischaemia-reperfusion;Myocardial ischemia-reperfusion injuries;Pathological process;Physiological process;Reactive oxygen species;Superoxide anions;animal cell;animal experiment;apoptosis;Article;biocompatibility;cardiac stem cell;cardiovascular disease;cell survival;column chromatography;confocal microscopy;controlled study;cytotoxicity;development;down regulation;flow cytometry;fluorescence imaging;fluorescence intensity;fluorescence microscopy;gene;gene silencing;genetic transfection;golph3 gene;H9c2(2-1) cell line;Hep-G2 cell line;high performance liquid chromatography;human;human cell;imaging;immunoblotting;limit of detection;liquid chromatography-mass spectrometry;mass spectrometry;MTT assay;myocardial ischemia reperfusion injury;nonhuman;nuclear magnetic resonance;oxidative stress;physiological stress;protein expression;upregulation;animal;diagnostic imaging;Golgi complex;metabolism;pathology;zebra fish;Cell death
摘要：
Superoxide anion (O2•-) is an important reactive oxygen species (ROS) and participates in various physiological and pathological processes in the organism. The O2•- burst induced by ischemia-reperfusion (I/R) is associated with cardiovascular disease and promotes the cell apoptosis. In this work, a turn-on type Golgi-targeting fluorescent probe Gol-Cou-O2•- was rationally designed for sensitive and selective detection of O2•-. The minimum detection limit concentration for O2•- was about 3.9 × 10−7 M in aqueous solution. Gol-Cou-O2•- showed excellent capacity of detecting exogenous and endogenous O2•- in living cells and zebrafish, and was also used to capture the up-regulated O2•- level during the duration of I/R process in cardiomyocytes. Golgi Phosphoprotein 3 (GOLPH3) is a potential Golgi stress marker protein and plays a key role in cells apoptosis during I/R. The fluorescence imaging and flow cytometry assay results indicated that silencing GOLPH3 through siRNA could give rise to the down-regulated O2•- level and alleviation of apoptosis in I/R myocardial cells. Thus, development of Gol-Cou-O2•- provides a diagnostic tool for myocardial oxidative stress injury and distinct insights on roles of GOLPH3 in myocardial I/R injury. © 2023 Elsevier B.V.
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The application of optical technology in the diagnosis and therapy of oxidative stress-mediated hepatic ischemia-reperfusion injury
作者：
Wang, Lijuan;Shao, Jiali;Su, Chen;Yang, Jinfeng
期刊：
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY ,2023年11:1133039 ISSN：2296-4185
通讯作者：
Yang, Jinfeng(yangjinfeng@hnca.org.cn)
作者机构：
[Wang, Lijuan] Univ South China, Hengyang Med Sch, Dept Med, Hengyang, Peoples R China.;[Shao, Jiali; Su, Chen; Yang, Jinfeng; Wang, Lijuan] Cent South Univ, Hunan Canc Hosp, Affiliated Canc Hosp, Xiangya Sch Med,Dept Anesthesiol, Changsha, Hunan, Peoples R China.
通讯机构：
[Yang, J.] D;Department of Anesthesiology, Hunan, China
关键词：
diagnosis;hepatic ischemia-reperfusion injury;optical technology;oxidative stress;treatment
摘要：
Hepatic ischemia-reperfusion injury (HIRI) is defined as liver tissue damage and cell death caused by reperfusion during liver transplantation or hepatectomy. Oxidative stress is one of the important mechanisms of HIRI. Studies have shown that the incidence of HIRI is very high, however, the number of patients who can get timely and efficient treatment is small. The reason is not hard to explain that invasive ways of detection and lack of timely of diagnostic methods. Hence, a new detection method is urgently needed in clinic application. Reactive oxygen species (ROS), which are markers of oxidative stress in the liver, could be detected by optical imaging and offer timely and effective non-invasive diagnosis and monitoring. Optical imaging could become the most potential tool of diagnosis of HIRI in the future. In addition, optical technology can also be used in disease treatment. It found that optical therapy has the function of anti-oxidative stress. Consequently, it has possibility to treat HIRI caused by oxidative stress. In this review, we mainly summarized the application and prospect of optical techniques in oxidative stress-induced by HIRI. Copyright © 2023 Wang, Shao, Su and Yang.
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英文
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Diallyl disulfide downregulating RhoGDI2 induces differentiation and inhibit invasion via the Rac1/Pak1/LIMK1 pathway in human leukemia HL-60 cells
作者：
Tan, Hui;Jiang, Yiling;Chen, Guangqun;Liu, Ran;Ling, Hui;...
期刊：
ENVIRONMENTAL TOXICOLOGY ,2023年38(5):1063-1077 ISSN：1520-4081
通讯作者：
Su, Qi(suqi1945@163.com)
作者机构：
[Chen, Guangqun; He, Jie; Jiang, Yiling; Yi, Lan; Tan, Hui; Liu, Ran; Ling, Hui; Qi, Su] Univ South China, Canc Res Inst, Hunan Prov Key Lab Canc Cellular & Mol Pathol, Hengyang, Peoples R China.;[Jiang, Yiling; Qi, Su] Univ South China, Affiliated Hosp 1, Dept Oncol, Hengyang, Peoples R China.;[Chen, Guangqun] Loudi Cent Hosp, Dept Oncol, Loudi, Peoples R China.;[Liu, Ran] First Hosp Changsha, Dept Pathol, Changsha, Peoples R China.;[Qi, Su] Univ South China, Canc Res Inst, Coll Hunan Prov, Key Lab Tumor Cellular & Mol Pathol,Hengyang Med C, 28 Changsheng Xi Rd, Hengyang 421001, Peoples R China.
通讯机构：
[Qi Su] H;Hunan Province Key Laboratory of Cancer Cellular and Molecular Pathology, Cancer Research Institute, University of South China, Hengyang, China<&wdkj&>Department of Oncology, First Affiliated Hospital, University of South China, Hengyang, China
关键词：
diallyl disulfide;HL-60 cells;LIMK1;Pak1;Rac1;RhoGDI2
摘要：
Leukemia is a type of disease in which hematopoietic stem cells proliferate clonally at the genetic level. We discovered previously by high-resolution mass spectrometry that diallyl disulfide (DADS), which is one of the effective ingredients of garlic, reduces the performance of RhoGDI2 from APL HL-60 cells. Although RhoGDI2 is oversubscribed in several cancer categories, the effect of RhoGDI2 in HL-60 cells has remained unexplained. We aimed to investigate the influence of RhoGDI2 on DADS-induced differentiation of HL-60 cells to elucidate the association among the effect of inhibition or over-expression of RhoGDI2 with HL-60 cell polarization, migration and invasion, which is important for establishing a novel generation of inducers to elicit leukemia cell polarization. Co-transfection with RhoGDI2-targeted miRNAs apparently decreases the malignant biological behavior of cells and upregulates cytopenias in DADS-treated HL-60 cell lines, which increases CD11b and decreases CD33 and mRNA levels of Rac1, PAK1 and LIMK1. Meanwhile, we generated HL-60 cell lines with high-expressing RhoGDI2. The proliferation, migration and invasion capacity of such cells were significantly increased by the treated with DADS, while the reduction capacity of the cells was decreased. There was a reduction in CD11b and an increase in CD33 production, as well as an increase in the mRNA levels of Rac1, PAK1 and LIMK1. It also confirmed that inhibition of RhoGDI2 attenuates the EMT cascade via the Rac1/Pak1/LIMK1 pathway, thereby inhibiting the malignant biological behavior of HL-60 cells. Thus, we considered that inhibition of RhoGDI2 expression might be a new therapeutic direction for the treatment of human promyelocytic leukemia. The anti-cancer property of DADS against HL-60 leukemia cells might be regulated by RhoGDI2 through the Rac1-Pak1-LIMK1 pathway, which provides new evidence for DADS as a clinical anti-cancer medicine. © 2023 Wiley Periodicals LLC.
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英文
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Construction of HClO activated near-infrared fluorescent probe for imaging hepatocellular carcinoma
作者：
Li, Songjiao;Wang, Peipei;Yang, Ke;Liu, Ying;Cheng, Dan;...
期刊：
Analytica Chimica Acta ,2023年1252:341009 ISSN：0003-2670
通讯作者：
He, Longwei(helongwei0110@163.com);Cheng, Dan(flychand0110@163.com)
作者机构：
[Liu, Ying; Yang, Ke; He, Longwei; Wang, Peipei; Li, Songjiao] Univ South China, Hengyang Med Sch, Canc Res Inst, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang 421001, Peoples R China.;[Cheng, Dan] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Res Inst, Hengyang, Peoples R China.
通讯机构：
[Dan Cheng] C;[Longwei He] H;L. He;Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Cancer Research Institute, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang, 421001, China;email: helongwei0110@163.com
关键词：
Animals;Carcinoma, Hepatocellular;Fluorescent Dyes;Hypochlorous Acid;Liver Neoplasms;Mice;Optical Imaging;Tumor Microenvironment;Biocompatibility;Cancer cells;Chlorine compounds;Cytology;Diagnosis;Diseases;Fluorescence imaging;Fluorescence spectroscopy;Infrared devices;Optical properties;Probes;fluorescent dye;hypochlorous acid;MBH 1;MBH melatonin;unclassified drug;fluorescent dye;hypochlorous acid;Fluorescent probes;Hepatocellular carcinoma;Hepatocytes;Hypochlorous acids;Imaging experiments;Liver cancers;Near Infrared;Near-infrared;Near-infrared fluorescent;Overall survival;animal cell;animal experiment;animal model;animal tissue;Article;biological monitoring;cancer surgery;controlled study;drug synthesis;early cancer diagnosis;female;Hep-G2 cell line;HK-2 [Human kidney] cell line;human;human cell;HUVEC cell line;in situ hybridization;in vivo study;L-02 cell line;live cell imaging;liver cell carcinoma;mouse;nonhuman;RAW 264.7 cell line;animal;diagnostic imaging;fluorescence imaging;liver cell carcinoma;liver tumor;metabolism;procedures;tumor microenvironment;Cells
摘要：
Hepatocellular carcinoma (HCC) is one of the most common malignancies in the liver with poor prognosis. In order to improve the prognosis and overall survival of patients with HCC, it is important to identify it at early stage and resect it precisely. Cell microenvironment, active compounds, and enzymes may change during the cancerization of hepatocytes. Hypochlorous acid (HClO), one of the most significant signal molecules in the cellular signaling pathway, plays an important role in many cellular processes. To detect and treat liver cancers, it is imperative to study how HClO levels change in hepatocytes. However, developing fluorescent probes specific to liver cells to detect HClO still challenging. Herein, we designed and synthesized a NIR hepatocyte-specific fluorescent probe (MBH-MT) that displayed excellent optical properties for detecting HClO in biological samples. Cell imaging experiment conducted with the unique probe MBH-MT, showed that the biocompatible sensor is capable of monitoring HClO and distinguishing normal cells from cancer cells (e.g., HepG2, HUVEC, RAW264.7, L02 and HK-2 cells). An organ imaging experiment with the probe MBH-MT demonstrated its effectiveness in diagnosing and imaging hepatocellular carcinoma in vivo. MBH-MT's in situ imaging also demonstrated that it can target and image mouse hepatocellular carcinomas. Furthermore, MBH-MT has also successfully been used to diagnose and guide liver cancer surgery early. In the future, we expect that this powerful tool may be help in the detection and imaging of hepatocellular carcinoma, which may affect a large number of people. © 2023 Elsevier B.V.
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英文
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Redox-Reversible Near-Infrared Fluorescent Probe for Imaging of Acute Kidney Oxidative Injury and Remedy
作者：
Ding, Yiteng;Zhong, Rongbin;Jiang, Renfeng;Yang, Xuefeng;He, Longwei;...
期刊：
ACS SENSORS ,2023年8(2):914-922 ISSN：2379-3694
通讯作者：
He, Longwei(helongwei0110@163.com);Cheng, Dan(flychand0110@163.com)
作者机构：
[Ding, Yiteng; Zhong, Rongbin; Yang, Xuefeng; Cheng, Dan] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hunan Prov Clin Res Ctr Metab Associated Fatty Liv, Hengyang 421002, Hunan, Peoples R China.;[He, Longwei; Jiang, Renfeng] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang Med Sch, Dept Pharm & Pharmacol, Hengyang 421002, Hunan, Peoples R China.;[Yuan, Lin; He, Longwei; Cheng, Dan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
通讯机构：
[Longwei He; Dan Cheng] H;Hunan Provincial Clinical Research Center for Metabolic Associated Fatty Liver Disease, Clinical Research Institute, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, Hunan, China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China<&wdkj&>Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang 421002, Hunan, China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China
关键词：
near-infrared;fluorescent probe;reversible imaging;peroxynitrite and glutathione;acute kidney injury
摘要：
Drug-induced acute kidney injury (DIAKI) is associated with high morbidity and mortality. It remains a diagnostic and therapeutic dilemma due to failure of providing unambiguous real-time feedback on nephrotoxicity, which is regarded as a serious problem in clinics. Herein, we report a reversible fluorescence probe, NRN, to monitor the ONOO-/GSH in an acute kidney injury model. The NRN near-infrared fluorescent probe features a big Stokes shift (83 nm), which was oxidized by ONOO- and reduced by succussive glutathione (GSH) with excellent selectivity and good sensitivity (detection limit: 418 nM and 0.28 mM, respectively). Taking the reversibility of NRN toward ONOO- and GSH, real-time evaluations in vivo with cisplatin (CP) alone and CP combined with acetaminophen-stimulated acute kidney injury and the following remedy process with l-carnitine were realized for the first time. The experiments revealed that acute kidney injury caused by combined drugs might be more serious and irreversible under certain conditions. Therefore, NRN could act as a potential tool for understanding oxidative stress-related DIAKI disease processes. © 2023 American Chemical Society.
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英文
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A mitochondrial-targeted near-infrared fluorescent probe for visualizing the fluctuation of hypochlorite acid in idiopathic pulmonary fibrosis mice
作者：
Li, Songjiao;Wang, Peipei;Liu, Ying;Yang, Ke;Zhong, Rongbin;...
期刊：
Analytica Chimica Acta ,2023年1239:340731 ISSN：0003-2670
通讯作者：
He, Longwei(helongwei0110@163.com);Cheng, Dan(flychand0110@163.com)
作者机构：
[Liu, Ying; Yang, Ke; He, Longwei; Wang, Peipei; Li, Songjiao] Univ South China, Canc Res Inst, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang Med Sch,Hunan Prov Key Lab Tumor Microenv, Hengyang 421001, Peoples R China.;[Zhong, Rongbin; Cheng, Dan] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Dan Cheng] C;[Longwei He] H;L. He;Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Cancer Research Institute, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang, 421001, China;email: helongwei0110@163.com
关键词：
Animals;Fluorescent Dyes;Hypochlorous Acid;Idiopathic Pulmonary Fibrosis;Inflammation;Mice;Microscopy, Fluorescence;Diagnosis;Fluorescence;Infrared devices;Mammals;Mitochondria;Probes;hypochlorous acid;nintedanib;reactive oxygen metabolite;fluorescent dye;hypochlorous acid;Chronic inflammatory disease;Fluorescent probes;Hypochlorite acid;Hypochlorous acids;Idiopathic pulmonary fibrosis;Mice models;Mortality rate;Near Infrared;Near-infrared;Near-infrared fluorescent;A-549 cell line;animal cell;animal experiment;animal model;Article;cellular distribution;chemical structure;concentration response;controlled study;drug cytotoxicity;drug efficacy;drug safety;fibrosing alveolitis;fluorescence;fluorometry;in vitro study;in vivo study;infant;mitochondrion;molecular probe;monitoring;mouse;NCI-H460 cell line;near infrared fluorescent probe;nonhuman;pathogenesis;peak-trough fluctuation;RAW 264.7 cell line;sensitivity analysis;synthesis;animal;diagnostic imaging;fibrosing alveolitis;fluorescence microscopy;inflammation;Chlorine compounds
摘要：
Idiopathic pulmonary fibrosis (IPF) is a chronic inflammatory disease destroying lungs irreversibly with high mortality rates. There are challenges in diagnosing IPF and treating it at an early stage. Mounting evidence suggests that hypochlorous acid (HClO) can help in diagnosing inflammation and relevant conditions. Pulmonary fibrosis is linked to the mitochondrial oxidative stress where excessive HClO production is a key molecular mechanism. Measuring mitochondrial HClO levels assists in the investigations of how the mitochondrial oxidative stress affects IPF. Herein, NIR-PTZ-HClO was developed and optimized as a probe for detecting fluctuations in HClO concentrations of cells and mice models through near-infrared (NIR) fluorescence. The probe featured large Stokes shift of 150 nm, NIR turn-on signal at 650 nm, high sensitivity (45-fold) and quick HClO detection (2 s). The probe is selective for HClO in the presence of range of other analytes. NIR-PTZ-HClO visualized both endogenous and exogenous HClO in living cells (RAW264.7, H460 and A549). The probe monitored HClO in mice models with IPF and moreover the HClO profile could be tracked during the IPF process. The probe also detected precipitous decrease in HClO levels in IPF mice treated with OFEV. NIR-PTZ-HClO probe has thus the potential for earlier diagnosis of lung fibrosis, thereby improving the treatment efficacy. © 2022
语种：
英文
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Enhancing the Selectivity of Leucine Aminopeptidase Near-Infrared Fluorescent Probes for Assisting in Surgical Tumor Resection
作者：
Zhong, Rongbin;Jiang, Renfeng;Zeng, Jiayu;Gong, Xiangyang;Yang, Xuefeng;...
期刊：
Analytical Chemistry ,2023年95(4):2428-2435 ISSN：0003-2700
通讯作者：
He, Longwei(helongwei0110@163.com);Cheng, Dan(flychand0110@163.com)
作者机构：
[Zeng, Jiayu; Yuan, Lin; Zhong, Rongbin; He, Longwei; Yang, Xuefeng; Cheng, Dan; Jiang, Renfeng] Univ South China, Affiliated Nanhua Hosp, Clin Res Inst,Hengyang Med Sch, Hunan Prov Clin Res Ctr Metab Assoc Fatty Liver Di, Hengyang 421002, Hunan, Peoples R China.;[Yuan, Lin; Gong, Xiangyang; Cheng, Dan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemomet, Changsha 410082, Peoples R China.
通讯机构：
[Longwei He; Dan Cheng] H;Hunan Provincial Clinical Research Center for Metabolic Associated Fatty Liver Disease, Clinical Research Institute, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, 421002 Hunan, China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082 Changsha, P. R. China<&wdkj&>Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang, 421002 Hunan, China
关键词：
Amino acids;Charge transfer;Fluorescence imaging;Fluorescence spectroscopy;Infrared devices;Mammals;Medical imaging;Probes;Analytes;Fluorescence imaging;Fluorescent probes;Imaging agent;Leucine aminopeptidase;Near-infrared fluorescent;Probe-based;Recognition units;Selective fluorescence;Tumor resection;Tumors
摘要：
Selective fluorescence imaging of analytes is a challenge for monitoring diseases as homologues interfere with the imaging agents. Leucine aminopeptidase (LAP), a kind of protease, is related to tumor pathogenesis. The known LAP fluorescent probes based on leucine recognition have limited selectivity. Herein, a selective t-butyl-alanine recognition unit for LAP through the ligand regulation strategy is prepared as a new near-infrared (NIR) fluorescent probe (DCM-LAP) having a large Stokes shift of 214 nm and a high sensitivity with a detection limit of 168 mU/L. DCM-LAP has an enhanced response toward LAP with NIR fluorescence at 656 nm based on intramolecular charge transfer. The probe is selective without being interfered with by biological enzymes including the aminopeptidase N (APN). DCM-LAP can image LAP activity in living cells. It can also visualize the cell invasion and migration processes. DCM-LAP is employed in the real-time imaging of LAP in tumor-bearing nude mice and guides in the accurate resection of breast tumors. It also distinguishes tumor tissues from normal with a high tumor-to-normal ratio (9.8). The DCM-LAP probe can thus assist in the investigations of LAP-associated clinical disease. © 2023 American Chemical Society.
语种：
英文
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Clinical application of serum-based proteomics technology in human tumor research
作者：
Li, Chen;Xiao, Juan;Wu, Shihua;Liu, Lu;Zeng, Xuemei;...
期刊：
Analytical Biochemistry ,2023年663:115031 ISSN：0003-2697
通讯作者：
Zhao, Qiang(276020102@qq.com);Zhang, Zhiwei(nhdxzzw@qq.com)
作者机构：
[Li, Chen; Zhao, Qiang; Zhang, Zhiwei; Liu, Lu] Univ South China, Dept Pathol, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Juan] Univ South China, Dept Otorhinolaryngol, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.;[Wu, Shihua] Second Hosp Shaoyang Coll, Dept Pathol, Hunan, Shaoyang 422000, Hunan, Peoples R China.;[Zhang, Zhiwei; Zeng, Xuemei] Univ South China, Canc Res Inst, Key Lab Canc Cellular & Mol Pathol Hunan Prov, Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Zhao, Qiang] Univ South China, Dept Pathol, Affiliated Hosp 1, 69 Chuanshan Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Qiang Zhao; Zhiwei Zhang] D;Q. Zhao;Department of Pathology, The First Affiliated Hospital of University of South China, Hengyang, 69 Chuanshan Road, Hunan, 421001, China;email: 276020102@qq.com;Z. Zhang
关键词：
Biomarkers, Tumor;Humans;Neoplasms;Proteome;Proteomics;Serum;Technology;Biomedical engineering;Clinical research;Molecular biology;Proteins;Tumors;plasma protein;proteome;tumor marker;proteome;tumor marker;Clinical application;Dynamic changes;Human serum;Human tumors;Human understanding;Pathological conditions;Physiological condition;Proteomic technologies;Serum;Supporting role;breast cancer;cancer research;clinical practice;colorectal cancer;extraction;hematologic malignancy;human;human tissue;liquid chromatography-mass spectrometry;liver cancer;lung cancer;matrix assisted laser desorption ionization time of flight mass spectrometry;prostate cancer;proteomics;Review;serum;stomach cancer;two dimensional gel electrophoresis;neoplasm;technology;Biomarkers
摘要：
The rapid development of proteomics technology in the past decades has led to further human understanding of tumor research, and in some ways, the technology plays a very important supporting role in the early detection of tumors. Human serum has been shown to contain a variety of proteins closely related to life activities, and the dynamic change in proteins can often reflect the physiological and pathological conditions of the body. Serum has the advantage of easy extraction, so the application of proteomics technology in serum has become a hot spot and frontier area for the study of malignant tumors. However, there are still many difficulties in the standardized use of proteomic technologies, which inevitably limit the clinical application of proteomic technologies due to the heterogeneity of human proteins leading to incomplete whole proteome populations, in addition to most serum protein markers being now not highly specific in aiding the early detection of tumors. Nevertheless, further development of proteomics technologies will greatly increase our understanding of tumor biology and help discover more new tumor biomarkers with specificity that will enable medical technology. © 2022 Elsevier Inc.
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英文
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Anticancer and Anti-Inflammatory Mechanisms of NOSH-Aspirin and Its Biological Effects
作者：
Zhou, Jun;Zeng, Weihong;Zeng, Ying;Li, Yukun;Xiao, Zheng;...
期刊：
Computational and Mathematical Methods in Medicine ,2022年2022(1):4463294 ISSN：1748-670X
通讯作者：
Xia, Jiliang(jiliangxia2009@163.com);Zeng, Xi(xzeng@usc.edu.cn)
作者机构：
[Li, Yukun; Xiao, Zheng; Zeng, Weihong; Zhou, Jun; Zeng, Ying; Zou, Juan; Peng, Lijun; Xia, Jiliang; Zeng, Xi] Univ South China, Canc Res Inst, Hengyang Med Sch, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Hunan, Peoples R China.;[Zeng, Ying] Univ South China, Sch Nursing, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Peng, Lijun] Univ South China, Affiliated Hosp 1, Dept Spine Surg, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Jiliang Xia; Xi Zeng] H;Hunan Province Key Laboratory of Tumor Cellular & Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China usc.edu.cn
摘要：
NOSH-Aspirin, which is generated from NO, H2S, and aspirin, affects a variety of essential pathophysiological processes, including anti-inflammatory, analgesic, antipyretic, antiplatelet, and anticancer properties. Although many people acknowledge the biological significance of NOSH-Aspirin and its therapeutic effects, the mechanism of action of NOSH-Aspirin and its regulation of tissue levels remains obscure. This is in part due to its chemical and physical features, which make processing and analysis difficult. This review focuses on the biological effects of NOSH-Aspirin and provides a comprehensive analysis to elucidate the mechanism underlying its disease-protective benefits. © 2022 Jun Zhou et al.
语种：
英文
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NIR-to-Vis Handheld Platforms for Detecting miRNA Level and Mutation Based on Sub-10 nm Sulfide Nanodots and HCR Amplification
作者：
Zhu, Yanli;Wang, Jikai;Xie, Haitao;Liu, Hailing;Liu, Shuangquan;...
期刊：
ACS Applied Materials & Interfaces ,2022年14(8):10212-10226 ISSN：1944-8244
通讯作者：
Wang, JK
作者机构：
[Zhu, Yanli] Hunan Univ Technol & Business, Sch Resources & Environm, Changsha 410205, Hunan, Peoples R China.;[Liu, Shuangquan; Xie, Haitao] Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Liu, Hailing] Wuhan Univ, Renmin Hosp, Dept Resp & Crit Care Med, Wuhan 430060, Hubei, Peoples R China.;[Wang, Jikai; Mi, Pengbing; He, Suisui; Wang, JK; Sun, Yiyang; He, Dongxiu; Wang, Jun] Univ South China, Hengyang Med Sch, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, JK ] U;Univ South China, Hengyang Med Sch, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.
关键词：
alkaline-earth sulfide nanomaterials;NIR-excited fluorescence;miRNA assay;mutant loci;single-nucleotide polymorphism
摘要：
Sub-10 nm monodisperse alkaline-earth sulfide nanodots (ASNDs) with bright near-infrared (NIR)-excitation fluorescence and adjustable emission wavelength were prepared by a thermal decomposition method for the first time. The ASNDs exhibited high NIR-to-vis conversion efficiency and served as multicolor fluorescent labels in the proposed miR-224 assay. Targeted detection of the miR-224 level and single-nucleotide variation in miR-224 was carried out on a smartphone-based platform using a hybridization chain reaction (HCR) amplification strategy. In the presence of miR-224, the ASND-labeled HCR probes self-assembled on the surface of the diagnosis kits, generating strong fluorescent signals linearly proportional to miR-224 contents in the range of 10-2000 fM. Significantly, mutations in miR-224 led to the variation in the fluorescence intensity ratio in RGB channels. Simultaneously, evident changes of fluorescent brightness and color were easily visualized by the naked eye, which enabled on-site discrimination of miR-224 with different mutant loci. This work provides a novel preparation approach for ultrasmall NIR excitation sulfide nanodots and reveals the potential of the as-synthesized ASNDs in point-of-care (POC) nucleic acid testing. Further, it may provide a handheld platform for miRNA single-nucleotide polymorphism analysis. © 2022 American Chemical Society
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英文
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A NIR-emissive probe with a remarkable Stokes shift for CO-releasing molecule-3 detection in cells and in vivo†
作者：
Li, Songjiao;Yang, Ke;Zeng, Jiayu;Xia, Yuqing;Cheng, Dan;...
期刊：
ANALYST ,2022年147(6):1169-1174 ISSN：0003-2654
通讯作者：
He, LW;Cheng, D
作者机构：
[He, LW; Zeng, Jiayu; Yang, Ke; He, Longwei; Li, Songjiao] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang Med Sch, Canc Res Inst,Dept Pharm & Pharmacol, Hengyang, Peoples R China.;[Cheng, Dan; Xia, Yuqing] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Res Inst, Hengyang, Peoples R China.;[Cheng, Dan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha, Peoples R China.
通讯机构：
[He, LW ; Cheng, D ] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang Med Sch, Canc Res Inst,Dept Pharm & Pharmacol, Hengyang, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Clin Res Inst, Hengyang, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha, Peoples R China.
关键词：
Animals;Carbon Monoxide;Diagnostic Imaging;Fluorescent Dyes;Mice;Organometallic Compounds;Biological systems;Controlled drug delivery;Fluorophores;Infrared devices;Mammals;Molecules;Neurodegenerative diseases;Probes;Targeted drug delivery;carbon monoxide;fluorescent dye;organometallic compound;tricarbonylchloro(glycinato)ruthenium(II);Alzheimers disease;Biologically active molecules;Cardiovascular disease;Chemical agent;Condition;Emissive probe;In-vivo;Living cell;Stokes shift;Therapeutic drugs;animal;chemistry;diagnostic imaging;mouse;Carbon monoxide
摘要：
Carbon monoxide (CO) is regarded as one of the most important gaseous transmitters, playing a vital role in biological systems; meanwhile, abnormal levels of CO can be correlated with conditions such as lung disease, Alzheimer's disease, and cardiovascular disease. CO-releasing molecules (CORMs) are chemical agents used to release CO as an endogenous, biologically active molecule in order to treat diseases. CO-releasing molecule-3 (CORM-3), as a convenient and safe CO donor and therapeutic drug molecule, has been widely used to release exogenous CO in living cells to study the physiological and pathological roles of CO in living systems. Herein, we designed a NIR-emitting probe (NIR-CORM-3) with a large Stokes shift based on a 4-(dimethylamino)cinnamaldehyde lepidine derived fluorophore. A 4-nitrobenzyl group was selected as the CORM-3 recognizing moiety, and the probe is able to selectively and sensitively respond to CORM-3 (within only 15 min). Upon encountering CORM-3, NIR-CORM-3 releases a fluorophore with a response at 670 nm, and it shows a remarkable Stokes shift (up to 250 nm). In addition, NIR-CORM-3 has low cytotoxicity and exhibits outstanding NIR imaging abilities in living cells and mice.
语种：
英文
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Pd-Catalyzed Aerobic Intermolecular 1,2-Diamination of Conjugated Dienes: Regio- and Chemoselective Synthesis of Piperazines and 2-Piperazinones
作者：
Liu, Huikang;Li, Yunyi;Yang, Zehua;Ge, Qianyi;Wu, Zhengxing;...
期刊：
CHEMISTRY-A EUROPEAN JOURNAL ,2022年28(57):e202201808- ISSN：0947-6539
通讯作者：
Wu, Zhengxing(wzxtheway@sjtu.edu.cn);Zhang, Wanbin(wanbin@sjtu.edu.cn)
作者机构：
[Wu, Zhengxing; Zhang, Wanbin; Li, Yunyi; Liu, Huikang] Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai Key Lab Mol Engn Chiral Drugs, 800 Dongchuan Rd, Shanghai 200240, Peoples R China.;[Ge, Qianyi; Yang, Zehua] Univ South China, Hengyang Med Sch, Inst Pharm & Pharmacol Sch Pharmaceut Sci, Hunan Prov Key Lab Tumor Microenvironm Respons Dr, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Dr. Zhengxing Wu; Prof. Wanbin Zhang] S;Z. Wu;Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, 800 Dongchuan Road, 200240, China;email: wzxtheway@sjtu.edu.cn;W. Zhang
关键词：
2-piperazinone;diamination;oxygen;palladium;piperazine
摘要：
Dinitrogen heterocycles are among the most important molecular structures, and the synthesis of these types of structures through intermolecular 1,2-diamination of olefins is a direct and efficient method. However, the types of nitrogen sources are mostly derived from ureas or arylamines, and nitrogen sources from aliphatic amines are still limited due to their distinct electronic and steric effects. Herein, we report a palladium-catalyzed aerobic intermolecular 1,2-diamination of conjugated dienes, using ethanediamine and α-amino amide derivatives as nitrogen sources respectively, for the synthesis of piperazines and 2-piperazinones in good yields (up to 95 %) and with high regio- and chemoselectivities. © 2022 Wiley-VCH GmbH.
语种：
英文
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Mechanisms of How Random Input Controls Bursting Gene Expression
作者：
Xiao, Sijia;Wang, Yan;Wang, Zhigang;Wang, Haohua
期刊：
Complexity ,2022年2022(1):5181333 ISSN：1076-2787
通讯作者：
Wang, Haohua(huazi8112@hainanu.edu.cn)
作者机构：
[Xiao, Sijia; Wang, Haohua; Wang, Zhigang] Hainan Univ, Sch Sci, Haikou 570228, Hainan, Peoples R China.;[Wang, Yan] Univ South China, Affiliated Hosp 1, Dept Neurol, Hengyang 421001, Hunan, Peoples R China.;[Wang, Haohua; Wang, Zhigang] Hainan Univ, Key Lab Engn Modeling & Stat Computat Hainan Prov, Haikou 570228, Hainan, Peoples R China.;[Wang, Haohua] Hainan Univ, Coll Forestry, Key Lab Genet & Germplasm Innovat Trop Special Fo, Minist Educ, Haikou 570228, Hainan, Peoples R China.
通讯机构：
[Haohua Wang] S;School of Sciences, Hainan University, Haikou 570228, Hainan, China hainu.edu.cn<&wdkj&>Hainan University, Key Laboratory of Engineering Modeling and Statistical Computation of Hainan Province, Haikou 570228, Hainan, China hainu.edu.cn<&wdkj&>Hainan University, Coll Forestry, Key Laboratory of Genetics & Germplasm Innovation Tropical Special Forest, Ministry of Education, Haikou 570228, Hainan, China meb.gov.tr
摘要：
The process of gene expression is affected by many extracellular stimulus signals, and the stochasticity of these signals reshapes gene expression. To adapt the fluctuation of the extracellular environment, genes have many strategies for augmenting their survival probability, frequency modulation, and amplitude modulation. However, it is unclear how genes utilize the stochasticity of signals to regulate gene expression and which strategy will be chosen to maximize cellular function. Here, we analyze a simple mechanistic model to clarify the effect of extracellular random input on gene expression and burst kinetics at different timescales. We can see that in different contexts, extracellular noise has different effects on downstream gene expression, effects which include the following: (1) extracellular noise will make the ON-OFF-state dwell time drift, which will influence the burst frequency and burst size of downstream gene expression under different modulation paradigms; (2) comparing the burst parameter or gene expression products under different modulation paradigms, we can see that the amplitude signal is more sensitive in the case of extracellular noise input, whereas the signal in noiseless conditions is more sensitive when the random input is a fast process, which indicates that the amplitude signal is a superior and common signal in gene expression; and (3) extracellular random input will change the bimodality for gene expression, but its influence is different for gene expression products under different modulation paradigms. These qualitative results reveal that extracellular random input can prompt the gene to achieve its function quickly under different modulation paradigms. © 2022 Sijia Xiao et al.
语种：
英文
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Double Signal Amplification Strategy for Dual-Analyte Fluorescent Aptasensors for Visualizing Cancer Biomarker Proteins
作者：
Zhu, Yanli;Wang, Jikai;Xie, Haitao;Fu, Chengxiao;Zhou, Jiecan;...
期刊：
Analytical Chemistry ,2022年94(29):10451-10461 ISSN：0003-2700
通讯作者：
Wang, Jikai(jkwang@hnu.edu.cn)
作者机构：
[Zhu, Yanli] Hunan Univ Technol & Business, Sch Resources & Environm, Changsha 410205, Peoples R China.;[Zhou, Jiecan; Xie, Haitao; Fu, Chengxiao] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Clin Lab,Hengyang Clin Pharmacol Res Ctr, Hengyang 421001, Peoples R China.;[Liu, Hailing] Wuhan Univ, Dept Respirator y & Crit Care Med, Renmin Hosp, Wuhan 430060, Peoples R China.;[Wang, Jikai; Sun, Yiyang; Zeng, Pengfei] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Inst Pharm & Pharmacol, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Jikai Wang] H;J. Wang;Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China;email: jkwang@hnu.edu.cn
关键词：
Alkalinity;Amplification;Color;Colorimetry;Diseases;Fluorescence;Image enhancement;Infrared devices;Sulfur compounds;Alkaline earth sulfides;Amplification strategy;Analytes;Aptasensors;Cancer biomarkers;Cancer diagnosis;Fluorescence assay;Nanodots;Ratiometric fluorescence;Signal amplifications;Biomarkers
摘要：
The simultaneous analysis of diversified biomarkers with high sensitivity and in a point-of-care (POC) manner is of great significance for facile and early cancer diagnosis. Herein, we develop a target amplification-assisted ratiometric fluorescence assay (TARFA) platform integrating the dual-amplification strategy and colorimetric readout technology for sensitive and specific detection of two malignancy-associated biomarkers. Meanwhile, the NIR-excited alkaline-earth sulfide nanodots (ASNDs) with an ultrasmall (<10 nm) diameter and tunable emission wavelength are employed to replace commonly UV/visible light-excited fluorescent labels to minimize background interference from the sample matrix. Unique advantages of the ASNDs, together with superiority of consecutive signal amplification of enzymatic target recycling (ETR) and hybridization chain reaction (HCR), realize the pg/mL-range detection limit in specifically recognizing the vascular endothelial growth factor (VEGF) and soluble interleukin-6 receptors (sIL-6R). The combination detection of the dual analyte exhibits an improved sensitivity for cancer diagnosis. The addition of the target biomarkers leads to an increasingly ratiometric RGB signal, and quantification based on the ratio-dependent signal is more reliable rather than measuring the absolute RGB signals. Moreover, perceptible color transformation makes the TARFA platform competent for visual analysis of the target analytes as convenient as reading the pH indicator strip, and hue-based image analysis also improves the method with fine precision by quantitatively identifying the visual color. This work provides a new kind of NIR-excited aptasensing platform with a low detection limit, high throughput, and great portability, which also highlights the potential of the ASNDs in biomolecular fluorescent labeling. © 2022 American Chemical Society.
语种：
英文
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The adhesion protein of Mycoplasma genitalium inhibits urethral epithelial cell apoptosis through CypA-CD147 activating PI3K/ Akt/NF-κB pathway
作者：
Liao, Yating;Peng, Kailan;Li, Xia;Ye, Youyuan;Liu, Peng;...
期刊：
Applied Microbiology and Biotechnology ,2022年106(19-20):6657-6669 ISSN：0175-7598
通讯作者：
Zeng, Yanhua(zengyihua21cn@usc.edu.cn)
作者机构：
[Liao, Yating; Liu, Peng; Ye, Youyuan; Peng, Kailan; Zeng, Yanhua; Li, Xia] Univ South China, Hengyang Med Sch, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang City 421001, Hunan, Peoples R China.;[Liao, Yating] Univ South China, Ctr Med Lab, Peoples Hosp 1, Chenzhou 423000, Peoples R China.
通讯机构：
[Yanhua Zeng] I;Y. Zeng;Institute of Pathogenic Biology, Hengyang Medical School, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang City, Hunan Province, 421001, China;email: zengyihua21cn@usc.edu.cn
关键词：
Animals;Annexin A5;Apoptosis;bcl-2-Associated X Protein;Carrier Proteins;Caspase 3;Cyclophilin A;Cytokines;Epithelial Cells;Humans;Mice;Mice, Inbred C57BL;Mycoplasma genitalium;NF-kappa B;Phosphatidylinositol 3-Kinases;Proto-Oncogene Proteins c-akt;Abstracting;Adhesion;Bacteria;Mammals;Proteins;caspase 3;CD147 antigen;cell adhesion molecule;cyclophilin A;immunoglobulin enhancer binding protein;lipocortin 5;membrane receptor;phosphatidylinositol 3 kinase;protein Bax;protein kinase B;carrier protein;cyclophilin A;cytokine;immunoglobulin enhancer binding protein;lipocortin 5;phosphatidylinositol 3 kinase;protein Bax;protein kinase B;Cell apoptosis;Cyclophilin A;Cyclophilin A-knockout mouse;Epithelial cells;Knockout mice;Mycoplasma genitalium;PI3K/Akt;PI3K/akt/NF-κb;SV-HUC-1;apoptosis;bacterium;cell component;flow cytometry;membrane;animal cell;animal experiment;apoptosis;Article;C57BL mouse;cell viability;controlled study;enzyme activity;epithelium cell;flow cytometry;host cell;knockout gene;knockout mouse;male;mouse;Mycoplasma genitalium;NF kB signaling;nonhuman;protein expression;signal transduction;SV-HUC-1 cell line;Western blotting;animal;apoptosis;epithelium cell;genetics;human;metabolism;Cell death
摘要：
Abstract: By interacting with the receptor on the host cells membrane, Mycoplasma genitalium, a prokaryotic bacterium primarily transmitted through sexual contact, can adhere to and even enter cells. The adhesion protein of M. genitalium (MgPa) plays a critical function in the adhering and subsequent invasion into host cells. Our prior studies verified that cyclophilin A (CypA) was the receptor of MgPa on human urethral epithelial cells (SV-HUC-1) membrane and could induce pro-inflammatory cytokines production through the CypA-CD147-ERK-NF-κB pathway. This research aims to understand how MgPa interacts with its membrane receptor CypA to cause apoptosis in host cells. We employed flow cytometry to see if MgPa prevents or enhances apoptosis of SV-HUC-1 cells. The apoptosis-related proteins such as Bax, caspase-3, and cleaved caspase-3 were assayed using Western blot. Results suggested that MgPa could inhibit the apoptosis of SV-HUC-1 cells. And we demonstrated that interference with the expression of CypA or CD147 significantly reversed the inhibitory effect of MgPa on SV-HUC-1 cells apoptosis, indicating that MgPa inhibited urothelial cells apoptosis through CypA/CD147. Furthermore, we discovered that MgPa regulates the PI3K/Akt/NF-κB pathway through CypA/CD147 to inhibit SV-HUC-1 cells apoptosis. Ultimately, the inhibitory effect of MgPa on the apoptosis of the urothelial epithelial cells extracted from CypA-knockout mice was validated by Annexin V/PI assay. The results corroborated that MgPa could also inhibit mouse urothelial epithelial cells apoptosis. In summary, we demonstrated that MgPa could inhibit SV-HUC-1 cells apoptosis via regulating the PI3K/Akt/NF-κB pathway through CypA/CD147, providing experimental evidence for elucidating the survival strategies of M. genitalium in host cells. Key points: • M. genitalium protein of adhesion inhibited human urethral epithelial cells apoptosis through CypA-CD147 activating the signal pathway of PI3K/Akt/NF-κB • The knockdown of CypA and CD147 could downregulate the M. genitalium -activated PI3K/Akt/NF-κB pathway in SV-HUC-1 cells • MgPa could inhibit the apoptosis of normal C57BL mouse primary urethral epithelial cells, but not for CypA-knockout C57BL mouse primary urethral epithelial cells. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Aging-Related Endothelial Progenitor Cell Dysfunction and Its Association with IL-17 and IL-23 in HFmrEF Patients
作者：
Zeng, Lijin;Zhang, Cong;Cai, Guoyi;Zhang, Bin;Huang, Zixia;...
期刊：
Oxidative Medicine and Cellular Longevity ,2022年2022(1):2281870 ISSN：1942-0900
通讯作者：
Luo, Liang(luoliang@mail.sysu.edu.cn)
作者机构：
[Yang, Zhen; Zeng, Lijin; Cai, Guoyi] Sun Yat Sen Univ, Affiliated Hosp 1, Div Emergency Med, Guangzhou, Peoples R China.;[Yang, Zhen; Zhang, Cong; Zeng, Lijin; Zhu, Yuanting] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Cardiol, Guangzhou, Peoples R China.;[Yang, Zhen; Zhang, Cong; Zeng, Lijin; Zhu, Yuanting] Sun Yat Sen Univ, NHC Key Lab Assisted Circulat, Guangzhou, Peoples R China.;[Zhang, Bin] Sun Yat Sen Univ, Jiangmen Cent Hosp, Dept Cardiovasc Dis, Affiliated Jiangmen Hosp, Jiangmen, Peoples R China.;[Zhang, Bin] Sun Yat Sen Univ, Jiangmen Cent Hosp, Clin Expt Ctr, Affiliated Jiangmen Hosp, Jiangmen, Peoples R China.
通讯机构：
[Hao He; Zixia Huang; Yuanting Zhu; Guoyi Cai; Cong Zhang; Liang Luo; Zhen Yang; Lijin Zeng; Bin Zhang; Mingyue Wu] D;Division of Emergency Medicine,The First Affiliated Hospital,Sun Yat-sen University,China<&wdkj&>Department of Cardiology,The First Affiliated Hospital,Sun Yat-sen University,China<&wdkj&>NHC Key Laboratory on Assisted Circulation (Sun Yat-sen University),Guangzhou,null,China<&wdkj&>Division of Emergency Medicine,The First Affiliated Hospital,Sun Yat-sen University,China<&wdkj&>Department of Cardiovascular Disease,Jiangmen Central Hospital,Affiliated Jiangmen Hospital of Sun Yat-sen University,China<&wdkj&>Clinical Experimental Center,Jiangmen Central Hospital,Affiliated Jiangmen Hospital of Sun Yat-sen University,China<&wdkj&>Department of Cardiology,The Fifth Affiliated Hospital of Zunyi Medical University,Zhuhai,China<&wdkj&>Department of Critical Care Medicine,Seventh Affiliated Hospital,Sun Yat-sen University,China<&wdkj&>Department of Anesthesiology,The Second Affiliated Hospital,Nanhua University,China<&wdkj&>Department of Cardiology,The First Affiliated Hospital,Sun Yat-sen University,China<&wdkj&>NHC Key Laboratory on Assisted Circulation (Sun Yat-sen University),Guangzhou,null,China
关键词：
Pathology;Cell dysfunction;Cell functions;Cells adhesion;Ejection fraction;Endothelial progenitor cells;Heart failure;Interleukin-17;Proliferative activity;Risk factors;Systemic inflammation;Adhesives;angiotensin receptor antagonist;beta adrenergic receptor blocking agent;dipeptidyl carboxypeptidase inhibitor;interleukin 17;interleukin 23;interleukin 17;interleukin 23;adult;aged;aging;Article;cell adhesion;cell function;cell migration;cell proliferation;controlled study;endothelial progenitor cell;female;heart failure;heart failure with mildly reduced ejection fraction;heart failure with mildly reduced ejection fraction;human;human cell;inflammation;major clinical study;male;protein blood level;aging;heart failure;metabolism;Adhesion;Age;Aging;Failure;Methods;Migration;Plasma;Scores;Aging;Endothelial Progenitor Cells;Heart Failure;Humans;Inflammation;Interleukin-17;Interleukin-23
摘要：
Background. Aging is an independent risk factor for heart failure (HF), and endothelial progenitor cell (EPC) function decreases with aging. Here, we further investigated whether age has a detrimental effect on circulating EPC function in HF with mildly reduced ejection fraction (HFmrEF) and its relationship with systemic inflammation. Methods. 58 HFmrEF patients were recruited. The adhesive, migrative, and proliferative activities of circulating EPCs, MAGGIC scores, and plasma interleukin (IL)-17 and IL-23 levels of these patients were assessed. Results. Older patients with HFmrEF had higher MAGGIC scores and lower circulating EPC adhesion, migration, and proliferation than younger patients. The similar tendency was observed in plasma IL-17 and IL-23 levels. The EPC functions were negatively associated with MAGGIC scores and plasma IL-17 or IL-23 levels. Conclusions. In patients with HFmrEF, aging leads to attenuated circulating EPC function, which is correlated with disease severity and systemic inflammation. The present investigation provides some novel insights into the mechanism and intervention targets of HFmrEF. © 2022 Lijin Zeng et al.
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英文
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Hydrogen Sulfide Attenuates the Cognitive Dysfunction in Parkinson's Disease Rats via Promoting Hippocampal Microglia M2 Polarization by Enhancement of Hippocampal Warburg Effect
作者：
Tian, Qing;Tang, Hui-Ling;Tang, Yi-Yun;Zhang, Ping* ;Kang, Xuan;...
期刊：
Oxidative Medicine and Cellular Longevity ,2022年2022(1):2792348 ISSN：1942-0900
通讯作者：
Tang, Xiao-Qing;Zhang, Ping
作者机构：
[Tang, Xiao-Qing; Kang, Xuan; Tang, Hui-Ling; Tang, Yi-Yun; Tian, Qing] Univ South China, Hengyang Med Sch, Inst Neurosci, Hengyang Key Lab Neurodegenerat & Cognit Impairme, Hengyang 421001, Hunan, Peoples R China.;[Zou, Wei; Tang, Hui-Ling; Zhang, Ping] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Neurol, Hengyang 421001, Hunan, Peoples R China.;[Tang, Xiao-Qing; Kang, Xuan] Univ South China, Affiliated Hosp 1, Inst Neurol, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tang, Xiao-Qing; Zhang, Ping] U;Univ South China, Hengyang Med Sch, Inst Neurosci, Hengyang Key Lab Neurodegenerat & Cognit Impairme, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Neurol, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Inst Neurol, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
关键词：
Brain;Hydrogen sulfide;Neurodegenerative diseases;Polarization;Sodium compounds;Sulfur determination;Cognitive functions;Cognitive impairment;Microglia;Parkinson's disease;Rat model;Therapeutic targets;Warburg effects;Rats;deoxyglucose;hydrogen sulfide;interleukin 4;lactic acid;pentobarbital;rotenone;sodium hydrosulfide;transforming growth factor beta1;unclassified drug;water;hydrogen sulfide;animal cell;animal experiment;animal model;Article;cognition;cognitive defect;controlled study;drug mechanism;hippocampus;M1 macrophage;M2 macrophage;male;microglia;neuroprotection;nonhuman;novel object recognition test;Parkinson disease;protein expression;rat;rat model;receptor down regulation;receptor upregulation;spatial memory;Warburg effect;Y-maze test;animal;cognitive defect;drug effect;hippocampus;human;Parkinson disease;Sprague Dawley rat;Data;Decay;Hydrogen Sulfide;Identification;Polarization;Processing;Upgrading;Work;Animals;Cognitive Dysfunction;Hippocampus;Humans;Hydrogen Sulfide;Male;Microglia;Parkinson Disease;Rats;Rats, Sprague-Dawley
摘要：
Identification of innovative therapeutic targets for the treatment of cognitive impairment in Parkinson's disease (PD) is urgently needed. Hydrogen sulfide (H2S) plays an important role in cognitive function. Therefore, this work is aimed at investigating whether H2S attenuates the cognitive impairment in PD and the underlying mechanisms. In the rotenone- (ROT-) established PD rat model, NaHS (a donor of H2S) attenuated the cognitive impairment and promoted microglia polarization from M1 towards M2 in the hippocampus of PD rats. NaHS also dramatically upregulated the Warburg effect in the hippocampus of PD rats. 2-Deoxyglucose (2-DG, an inhibitor of the Warburg effect) abolished NaHS-upregulated Warburg effect in the hippocampus of PD rats. Moreover, the inhibited hippocampal Warburg effect by 2-DG abrogated H2S-excited the enhancement of hippocampal microglia M2 polarization and the improvement of cognitive function in ROT-exposed rats. Our data demonstrated that H2S inhibits the cognitive dysfunction in PD via promoting microglia M2 polarization by enhancement of hippocampal Warburg effect. © 2022 Qing Tian et al.
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英文
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