Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform

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成果类型：

期刊论文

作者：

Chen, Yulin;Gao, Dan;Zhu, Qingyun;Chu, Bizhu;Peng, Jie;...

通讯作者：

Gao, Dan(gaogao.dan@sz.tsinghua.edu.cn)

作者机构：

[Peng, Jie; Jiang, Yuyang; Gao, Dan; Chen, Yulin] Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, State Key Lab Chem Oncogen, Guangdong Prov Key Lab Chem Biol, Shenzhen 518055, Guangdong, Peoples R China.

[Zhu, Qingyun] Univ South China, Affiliated Hosp 1, Canc Res Inst, Hengyang Med Sch, Hengyang 421001, Peoples R China.

[Chu, Bizhu] Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Shenzhen 518060, Peoples R China.

[Wang, Jian] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Thorac Surg, Shenzhen 518020, Guangdong, Peoples R China.

[Wang, Jian; Liu, Liping] Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China.

通讯机构：

[Dan Gao] S

State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, China

语种：

英文

期刊：

ANALYST

ISSN：

0003-2654

年：

2023

卷：

148

期：

页码：

2387-2394

DOI：

10.1039/d3an00335c

基金类别：

Natural Science Foundation of Guangdong Province [2022A1515011437, 2020A1515010660]; Shenzhen Fundamental Research and Discipline Layout Project [JCYJ20180508152244835]; Research and Development Program in Key Areas of Guangdong Province, P.R. China [2019B020209009]

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

Exosomes are considered as promising biomarkers for early cancer diagnosis and prognosis. However, the majority of the research studies focused on a single type of exosomal biomarkers, which cannot comprehensively reflect the state of cancer for accurate diagnosis. To address this problem, we presented a ship-shaped microfluidic device containing a microcolumn array for simultaneous in situ detection of exosomal surface proteins and miRNAs. Exosomes were first captured in the microchannels modified with CD63 protein aptamer. Exosomal surface pr...

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Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform

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