作者机构：
[Wei, Yanhong] Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi, Department of Obstetrics and Gynecology, Department of reproductive medicine Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China. Electronic address: 2514138998@qq.com;[Huang, Qiumei] Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi, Department of Obstetrics and Gynecology, Department of reproductive medicine Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China. Electronic address: 17877325360@163.com;[Fu, Zhenhui] Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi, Department of Obstetrics and Gynecology, Department of reproductive medicine Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China. Electronic address: fuzhenhui10@163.com;[Wang, Rutong] Institute of Clinical Anatomy & Reproductive Medicine Department of Histology and Embryology Hengyang Medical School University of South China Hengyang, Hunan 421001, China. Electronic address: 18754723679@163.com;[Zhou, Runtang] Institute of Clinical Anatomy & Reproductive Medicine Department of Histology and Embryology Hengyang Medical School University of South China Hengyang, Hunan 421001, China. Electronic address: 2662580138@qq.com
通讯机构：
[Huang, Qiumei; Hu, Linlin; Fu, Zhenhui; Wei, Yanhong] K;[Wang, Rutong; Lei, Xiaocan; Zhou, Runtang] I;[Le, Jianghua] D;Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi, Department of Obstetrics and Gynecology, Department of reproductive medicine Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China. Electronic address:;Institute of Clinical Anatomy & Reproductive Medicine Department of Histology and Embryology Hengyang Medical School University of South China Hengyang, Hunan 421001, China. Electronic address:
关键词：
Gut microbiome;Male infertility;Metabolomics;Network pharmacology;Obesity;Pyrroloquinoline quinone
摘要：
This study employed an obese mouse model to investigate the effects of pyrroloquinoline quinone (PQQ) intervention on reproductive physiology and glycolytic pathways. Our findings demonstrate that PQQ markedly enhanced glycolytic activity in obese mice, promoting glucose breakdown and metabolism to improve energy supply efficiency. These metabolic improvements correlated with significant support for reproductive system functionality. Mechanistic analyses revealed PQQ's potential to augment mitochondrial respiration, ameliorate mitochondrial dysfunction, and counteract obesity-associated inflammation, thereby preserving the balance between fatty acid degradation and integrated glycolysis-cholesterol metabolism. Collectively, this work provides novel insights into PQQ's molecular mechanisms for promoting glycolysis and ameliorating infertility in obese males.
This study employed an obese mouse model to investigate the effects of pyrroloquinoline quinone (PQQ) intervention on reproductive physiology and glycolytic pathways. Our findings demonstrate that PQQ markedly enhanced glycolytic activity in obese mice, promoting glucose breakdown and metabolism to improve energy supply efficiency. These metabolic improvements correlated with significant support for reproductive system functionality. Mechanistic analyses revealed PQQ's potential to augment mitochondrial respiration, ameliorate mitochondrial dysfunction, and counteract obesity-associated inflammation, thereby preserving the balance between fatty acid degradation and integrated glycolysis-cholesterol metabolism. Collectively, this work provides novel insights into PQQ's molecular mechanisms for promoting glycolysis and ameliorating infertility in obese males.
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2026年344(Pt 1):126623 ISSN：1386-1425
通讯作者：
Longwei He<&wdkj&>Songjiao Li
作者机构：
[Deng, Min; Ai, Siwei; Liu, Ying; Zhang, Hailin; Zhai, Zibo; Li, Songjiao] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang 421002, China;Department of Gastroenterology, Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, PR China;School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China;[Cheng, Dan] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>Department of Gastroenterology, Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, PR China;[He, Longwei] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China
通讯机构：
[Longwei He; Songjiao Li] H;Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Department of Pharmacy and Pharmacology, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China
摘要：
In osteoarthritis , hypochlorous acid (HClO)—a critical biomarker of inflammation—and neutrophil elastase (NE)—a key mediator of inflammatory responses—undergo significant changes. Fluorescent probes , which offer high sensitivity and real-time visualization, have been widely employed for osteoarthritis imaging. However, most existing probes are designed to detect only a single biomarker, which can lead to false-positive signals in complex biological environments. In contrast, dual-locked fluorescent probes, which require activation by two distinct biomarkers, offer improved specificity and reliability. These probes are particularly advantageous for multiplexed detection in arthritis-related imaging. To date, no dual-responsive fluorescent probe targeting both HClO and NE has been reported. In this study, we introduce Cou-HN, a dual-locked fluorescent probe that exhibits a significant fluorescence enhancement at 470 nm only when both HClO and NE are present simultaneously. Neither HClO nor NE alone is sufficient to trigger a notable fluorescence response. This dual-activation strategy provides Cou-HN with superior imaging accuracy compared to single-locked probes. Both in vitro and in vivo experiments confirm its excellent performance, highlighting its promise for more precise diagnosis and monitoring of osteoarthritis.
In osteoarthritis , hypochlorous acid (HClO)—a critical biomarker of inflammation—and neutrophil elastase (NE)—a key mediator of inflammatory responses—undergo significant changes. Fluorescent probes , which offer high sensitivity and real-time visualization, have been widely employed for osteoarthritis imaging. However, most existing probes are designed to detect only a single biomarker, which can lead to false-positive signals in complex biological environments. In contrast, dual-locked fluorescent probes, which require activation by two distinct biomarkers, offer improved specificity and reliability. These probes are particularly advantageous for multiplexed detection in arthritis-related imaging. To date, no dual-responsive fluorescent probe targeting both HClO and NE has been reported. In this study, we introduce Cou-HN, a dual-locked fluorescent probe that exhibits a significant fluorescence enhancement at 470 nm only when both HClO and NE are present simultaneously. Neither HClO nor NE alone is sufficient to trigger a notable fluorescence response. This dual-activation strategy provides Cou-HN with superior imaging accuracy compared to single-locked probes. Both in vitro and in vivo experiments confirm its excellent performance, highlighting its promise for more precise diagnosis and monitoring of osteoarthritis.
作者机构：
[Yu, Mengyao; Zhang, Yifang] Department of Pathophysiology, Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan Province, International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Hengyang Medical School, University of South China, Hengyang, China;[Chen, Yan] Department of Clinical Medicine, Hengyang Medical School, University of South China, Hengyang, China;[Huang, Ji] Department of Pathophysiology, Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan Province, International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Hengyang Medical School, University of South China, Hengyang, China. Electronic address: huangjicpu@hotmail.com
通讯机构：
[Huang, Ji] D;Department of Pathophysiology, Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan Province, International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Hengyang Medical School, University of South China, Hengyang, China. Electronic address:
摘要：
High-density lipoprotein (HDL) plays a key role in reverse cholesterol transport (RCT), traditionally associated with cardiovascular protection. However, while low HDL-cholesterol (HDL-C) levels correlate with increased cardiovascular risk, therapeutic interventions raising HDL-C (e.g., niacin, fibrates) have failed to reduce cardiovascular events. Recent evidence reveals a U-shaped relationship between HDL-C and mortality, with both excessively high and low levels conferring risk—indicating functional impairment as the critical determinant. Consequently, focus has shifted from HDL-C quantification to functional assessment: (1) Cholesterol efflux capacity (CEC) exhibits a stronger inverse correlation with cardiovascular risk than HDL-C; (2) HDL particle number (HDL-P) outperforms HDL-C in predicting cardiovascular events; (3) HDL subclass heterogeneity (e.g., HDL2, HDL3), where composition and distribution determine the protective functions of HDL particles. This review synthesizes evidence demonstrating that comprehensively assessing HDL functionality—including quality metrics, particle concentration, and subclass distribution—provides superior cardiovascular risk assessment. Future research must prioritize restoring or enhancing HDL function rather than merely increasing its concentration.
High-density lipoprotein (HDL) plays a key role in reverse cholesterol transport (RCT), traditionally associated with cardiovascular protection. However, while low HDL-cholesterol (HDL-C) levels correlate with increased cardiovascular risk, therapeutic interventions raising HDL-C (e.g., niacin, fibrates) have failed to reduce cardiovascular events. Recent evidence reveals a U-shaped relationship between HDL-C and mortality, with both excessively high and low levels conferring risk—indicating functional impairment as the critical determinant. Consequently, focus has shifted from HDL-C quantification to functional assessment: (1) Cholesterol efflux capacity (CEC) exhibits a stronger inverse correlation with cardiovascular risk than HDL-C; (2) HDL particle number (HDL-P) outperforms HDL-C in predicting cardiovascular events; (3) HDL subclass heterogeneity (e.g., HDL2, HDL3), where composition and distribution determine the protective functions of HDL particles. This review synthesizes evidence demonstrating that comprehensively assessing HDL functionality—including quality metrics, particle concentration, and subclass distribution—provides superior cardiovascular risk assessment. Future research must prioritize restoring or enhancing HDL function rather than merely increasing its concentration.
期刊：
Sensors and Actuators B-Chemical,2026年447:138814 ISSN：0925-4005
通讯作者：
Jia Zhou
作者机构：
[Zangfei Xie; Xiang Li] MOE Key Lab of Rare Pediatric Diseases, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421002, China;[Jia Huang; Wei Zeng; Jia Zhou] Department of Ultrasound Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China;Department of Gastroenterology, Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China;School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China;[Dan Cheng] Department of Gastroenterology, Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China
通讯机构：
[Jia Zhou] D;Department of Ultrasound Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China
摘要：
Inhalation lung injury (ILI) is an inflammation-driven disease primarily mediated by oxidative stress. Accordingly identifying oxidative stress-related factors is crucial for early diagnosis and timely intervention of ILI. Hydrogen peroxide (H 2 O 2 ), one of the important reactive oxygen species (ROS), has been shown to be a key regulator in the onset and progression of ILI, with its levels dynamically reflecting the extent of oxidative stress. The selective detection of H 2 O 2 using fluorescence imaging technology is a promising method for ILI detection. Herein, the fluorescent probe SRHFP based on a H 2 O 2 -triggered structural self-rebuilding procedure including successive three-step oxidation, self-immolation and covalent bond assembly has been developed for real-time H 2 O 2 monitoring. SRHFP performs the advantageous optical properties, such as low detection limit of 0.1 μM under physiological conditions, NIR emission band centered at 660 nm to avoid tissue autofluorescence, large Stokes shift of 230 nm to minimize fluorescence self-quenching, and remarkable stability. Probe SRHFP has capability of sensitively monitoring both endogenous and exogenous H 2 O 2 in A549 cells and accurately detecting various toxic chemical gases-induced lung injury model cells. What’s more, SRHFP possesses the characteristic of priority lung accumulation and was successfully applied to monitor the H 2 O 2 elevation in the lungs of ILI model mice induced by inhalation of toxic gas and the decrease of H 2 O 2 concentration in dexamethasone-treated mice, confirming the strong correlation between H 2 O 2 levels and ILI pathological process. This probe enables dynamic visualization of ILI progression and holds significant potential as a diagnostic tool for inflammation-related lung diseases.
Inhalation lung injury (ILI) is an inflammation-driven disease primarily mediated by oxidative stress. Accordingly identifying oxidative stress-related factors is crucial for early diagnosis and timely intervention of ILI. Hydrogen peroxide (H 2 O 2 ), one of the important reactive oxygen species (ROS), has been shown to be a key regulator in the onset and progression of ILI, with its levels dynamically reflecting the extent of oxidative stress. The selective detection of H 2 O 2 using fluorescence imaging technology is a promising method for ILI detection. Herein, the fluorescent probe SRHFP based on a H 2 O 2 -triggered structural self-rebuilding procedure including successive three-step oxidation, self-immolation and covalent bond assembly has been developed for real-time H 2 O 2 monitoring. SRHFP performs the advantageous optical properties, such as low detection limit of 0.1 μM under physiological conditions, NIR emission band centered at 660 nm to avoid tissue autofluorescence, large Stokes shift of 230 nm to minimize fluorescence self-quenching, and remarkable stability. Probe SRHFP has capability of sensitively monitoring both endogenous and exogenous H 2 O 2 in A549 cells and accurately detecting various toxic chemical gases-induced lung injury model cells. What’s more, SRHFP possesses the characteristic of priority lung accumulation and was successfully applied to monitor the H 2 O 2 elevation in the lungs of ILI model mice induced by inhalation of toxic gas and the decrease of H 2 O 2 concentration in dexamethasone-treated mice, confirming the strong correlation between H 2 O 2 levels and ILI pathological process. This probe enables dynamic visualization of ILI progression and holds significant potential as a diagnostic tool for inflammation-related lung diseases.
期刊：
Sensors and Actuators B-Chemical,2026年446:138728 ISSN：0925-4005
通讯作者：
Longwei He
作者机构：
[Jia Huang; Wanting Zhang; Wei Zeng; Jia Zhou] Department of Ultrasound Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China;MOE Key Lab of Rare Pediatric Diseases, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421002, China;[Yuanyuan Wang] Department of Gastroenterology, Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China;School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China;[Jiaoli Zhang] Department of Blood Transfusion, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China
通讯机构：
[Longwei He] D;Department of Ultrasound Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>MOE Key Lab of Rare Pediatric Diseases, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421002, China<&wdkj&>School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China
摘要：
Triple-negative breast cancer (TNBC) is often referred to as the ‘king of breast cancers’ due to its extremely poor prognosis. As an emerging cancer treatment, photodynamic therapy (PDT) has become a focal point of current research because of its high selectivity, minimal invasiveness, and low side effects. Thus, developing high-performance agents to effectively treat TNBC using PDT is crucial. Here, we designed a new near-infrared activatable photosensitizer ( NIR-NBB ) with mitochondria-targeted capability, which was composed by a near-infrared photosensitizer NPA-NH 2 , and a ketoamide recognition site for peroxynitrite (a key tumor indicator). This agent can sense ONOO - with high sensitivity and specifically accumulates in the mitochondria of tumor cells. Under 660 nm laser irradiation, it would promote the accumulation of high levels of cytotoxic ROS, triggering extensive mitophagy, thereby amplifying lipid peroxidation and enhancing endogenous ferroptosis, thus achieving optimal antitumor effects. This agent would hold promise as a potential diagnostic and therapeutic tool for cancer.
Triple-negative breast cancer (TNBC) is often referred to as the ‘king of breast cancers’ due to its extremely poor prognosis. As an emerging cancer treatment, photodynamic therapy (PDT) has become a focal point of current research because of its high selectivity, minimal invasiveness, and low side effects. Thus, developing high-performance agents to effectively treat TNBC using PDT is crucial. Here, we designed a new near-infrared activatable photosensitizer ( NIR-NBB ) with mitochondria-targeted capability, which was composed by a near-infrared photosensitizer NPA-NH 2 , and a ketoamide recognition site for peroxynitrite (a key tumor indicator). This agent can sense ONOO - with high sensitivity and specifically accumulates in the mitochondria of tumor cells. Under 660 nm laser irradiation, it would promote the accumulation of high levels of cytotoxic ROS, triggering extensive mitophagy, thereby amplifying lipid peroxidation and enhancing endogenous ferroptosis, thus achieving optimal antitumor effects. This agent would hold promise as a potential diagnostic and therapeutic tool for cancer.
摘要：
Introduction Glucosylceramidase beta 1 (GBA1) mutations are a genetic risk factor for Parkinson's disease (PD), though most carriers do not develop the disease. This study aimed to identify exposure factors linked to PD in GBA1 carriers and assess clinical features and the probability of prodromal PD in non-manifesting carriers.
Glucosylceramidase beta 1 (GBA1) mutations are a genetic risk factor for Parkinson's disease (PD), though most carriers do not develop the disease. This study aimed to identify exposure factors linked to PD in GBA1 carriers and assess clinical features and the probability of prodromal PD in non-manifesting carriers.
Methods Data from the Parkinson's Disease & Movement Disorders Multicenter Database and Collaborative Network in China was used, including 59 GBA1 non-manifesting carriers, 62 controls, and 107 GBA1-associated PD, of whom 81 were in the early stage. Exposure factors included pesticide/solvent exposure, smoking, alcohol, and tea consumption. Logistic regression assessed the association between exposure factors and PD. Clinical characteristics were evaluated using multiple scales, relevant markers were collected based on the Movement Disorders Society criteria. A naive Bayesian classifier method determined the probability of prodromal PD in GBA1 non-manifesting carriers and controls.
Data from the Parkinson's Disease & Movement Disorders Multicenter Database and Collaborative Network in China was used, including 59 GBA1 non-manifesting carriers, 62 controls, and 107 GBA1-associated PD, of whom 81 were in the early stage. Exposure factors included pesticide/solvent exposure, smoking, alcohol, and tea consumption. Logistic regression assessed the association between exposure factors and PD. Clinical characteristics were evaluated using multiple scales, relevant markers were collected based on the Movement Disorders Society criteria. A naive Bayesian classifier method determined the probability of prodromal PD in GBA1 non-manifesting carriers and controls.
Results After adjusting for sociodemographic variables, pesticide/solvent exposure was positively associated with PD in GBA1 carriers (OR 8.40; 95 % CI 2.50–28.20), while smoking was inversely associated with PD (OR 0.18; 95 % CI 0.05–0.62). Rapid eye movement sleep behavior disorder, constipation, hyposmia, and cognitive deficits were more severe in early-stage GBA1-associated PD than in carriers and controls. Clinical symptoms and the probability of prodromal PD were similar between carriers and controls.
After adjusting for sociodemographic variables, pesticide/solvent exposure was positively associated with PD in GBA1 carriers (OR 8.40; 95 % CI 2.50–28.20), while smoking was inversely associated with PD (OR 0.18; 95 % CI 0.05–0.62). Rapid eye movement sleep behavior disorder, constipation, hyposmia, and cognitive deficits were more severe in early-stage GBA1-associated PD than in carriers and controls. Clinical symptoms and the probability of prodromal PD were similar between carriers and controls.
Conclusions PD in GBA1 carriers is closely linked to exposure factors. Early-stage GBA1-associated PD shows significant prodromal symptoms, which are not evident in carriers. The probability of prodromal PD in carriers is similar to that in controls.
PD in GBA1 carriers is closely linked to exposure factors. Early-stage GBA1-associated PD shows significant prodromal symptoms, which are not evident in carriers. The probability of prodromal PD in carriers is similar to that in controls.
摘要：
BACKGROUND: Neuronal senescence is a common pathological feature of various neurodegenerative diseases, with ferroptosis playing a significant role. This study aims to investigate the role of ErbB4 receptor activation in preventing D-Galactose (D-gal)-induced neuronal senescence. METHODS: Mice subjected to D-gal-induced aging were administered a small molecule ErbB4 receptor agonist (E4A), identified via virtual screening, melatonin, or a combination of both. Behavioral assessments were conducted to evaluate therapeutic efficacy in memory and cognitive functions. Immunofluorescence staining, western blot, and biochemical assays were primarily employed to assess changes in both senescence- and ferroptosis-related molecules in mouse hippocampal tissues in response to each treatment. Additionally, mouse hippocampal HT22 neuronal cell cultures were utilized to corroborate the in vivo findings. RESULTS: The targeted activation of ErbB4 receptor by E4A significantly ameliorated the behavioral deficits induced by D-gal in mice, demonstrating an effect comparable to that of melatonin, a natural inhibitor of in vivo senescence and ferroptosis. Both E4A and melatonin mitigated D-gal-induced aging in hippocampal neurons of mice. This was evidenced by the upregulation of Lamin B1 and the downregulation of P53, P21, P16, GFAP, and Iba-1 expression levels. Moreover, D-gal treatment markedly decreased the protein expression of the ferroptosis inhibitor Nrf2 while augmenting the expression of the ferroptosis promoter TFRC. These alterations were partially reversed by the individual administration of E4A and melatonin. In vitro studies further corroborated that D-gal treatment significantly and concurrently induced the expression of senescence markers and ferroptosis promoters. However, both E4A and melatonin were able to significantly reverse these changes. Additionally, E4A markedly ameliorated Erastin-induced ferroptosis in mouse hippocampal neuronal cells. CONLUSION: Our findings suggest that targeted activation of ErbB4 receptor may be a viable strategy for treating neuronal senescence by inhibiting ferroptosis, thereby offering a potential therapeutic avenue for senescence-associated neurodegenerative diseases.
通讯作者：
Lan Luo<&wdkj&>Lifang Huang<&wdkj&>Zhenhua Ming
作者机构：
[Luo, Lan; Huang, Lifang; Ming, Zhenhua] Department of Rehabilitation, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, Hunan Province, China;[Liu, Yanhui] Teaching and Research Section of Clinical Nursing, Neurosurgical Intensive Care Unit, Xiangya Hospital, Central South University, Changsha, Hunan Province, China;[Cao, Jiawei] Department of Anesthesiology, Changsha Central Hospital, Affiliated to the University of South China, Changsha, Hunan Province, China. Electronic address: doctcjw@163.com
通讯机构：
[Lan Luo; Lifang Huang; Zhenhua Ming] D;Department of Rehabilitation, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, Hunan Province, China
关键词：
This study followed the PRISMA (Moher et al.;2009) and ENTREQ checklist (Tong et al.;and registered with PROSPERO (CRD42024535641). PubMed;Medline;Embase;Scopus;CINAHL;PsycINFO;and Web of Science were searched for qualitative studies on SCI-related NP from inception to May 30;2024. A combination of subject terms (MeSH terms) and free text keywords was used;with Boolean operators applied to combine the terms effectively. Search terms used were: spinal cord;nerv;neuro*;pain
摘要：
Background Neuropathic pain (NP), characterized by variable symptoms, treatment resistance, and substantial physical/psychological burdens, impacts 40%-60% of spinal cord injury (SCI) patients. Due to the unpredictable onset and pervasive nature of NP, timely intervention cannot be made, so self-management strategies become necessary. However, limitations are present in these strategies. Therefore, we conducted a meta-synthesis of qualitative studies to explore how the complexity of NP (physical, emotional, and social challenges) interferes with self-management effectiveness, thereby guiding tailored, evidence-based management for NP.
Neuropathic pain (NP), characterized by variable symptoms, treatment resistance, and substantial physical/psychological burdens, impacts 40%-60% of spinal cord injury (SCI) patients. Due to the unpredictable onset and pervasive nature of NP, timely intervention cannot be made, so self-management strategies become necessary. However, limitations are present in these strategies. Therefore, we conducted a meta-synthesis of qualitative studies to explore how the complexity of NP (physical, emotional, and social challenges) interferes with self-management effectiveness, thereby guiding tailored, evidence-based management for NP.
Objective A meta-synthesis of qualitative studies was conducted on the SCI patients' NP experience and self-management strategies.
A meta-synthesis of qualitative studies was conducted on the SCI patients' NP experience and self-management strategies.
Methods PubMed, Medline, Embase, Scopus, CINAHL, PsycINFO, and Web of Science were searched for qualitative studies on NP in SCI patients from inception to May 30, 2024. The quality of the included studies was evaluated based on the JBI Center for Evidence-Based Health Care Quality Assessment Standards for Qualitative Research, and the results were synthesized using a thematic synthesis approach.
PubMed, Medline, Embase, Scopus, CINAHL, PsycINFO, and Web of Science were searched for qualitative studies on NP in SCI patients from inception to May 30, 2024. The quality of the included studies was evaluated based on the JBI Center for Evidence-Based Health Care Quality Assessment Standards for Qualitative Research, and the results were synthesized using a thematic synthesis approach.
Results Twelve studies were included, and 49 codes were extracted, which were synthesized into three major themes (real experiences and feelings; treatment and management of pain; desires and requirements) and 10 subthemes (the characteristics of pain; the impact on life; the impact on psychology; balancing between pain and side effects is difficult; nonpharmacological alternative therapies are inadequate; self-management strategies are necessary; access to information and expertise; understanding and respect from physicians; improved structures in pain management; and strengthen social support).
Twelve studies were included, and 49 codes were extracted, which were synthesized into three major themes (real experiences and feelings; treatment and management of pain; desires and requirements) and 10 subthemes (the characteristics of pain; the impact on life; the impact on psychology; balancing between pain and side effects is difficult; nonpharmacological alternative therapies are inadequate; self-management strategies are necessary; access to information and expertise; understanding and respect from physicians; improved structures in pain management; and strengthen social support).
Conclusions NP seriously affects the physical and psychological health of patients, but few self-management strategies can be used currently. Therefore, more self-management strategies should be provided for patients with NP, and healthcare providers including nurses should enrich their knowledge of NP management to give patients more help and support.
NP seriously affects the physical and psychological health of patients, but few self-management strategies can be used currently. Therefore, more self-management strategies should be provided for patients with NP, and healthcare providers including nurses should enrich their knowledge of NP management to give patients more help and support.
Nursing Practice Implications (1) Implementing comprehensive pain assessments encompassing physical, functional, and psychological impacts; (2) Providing tailored education and training on diverse personalized self-management strategies; (3) Prioritizing psychosocial support and counseling, and facilitating access to resources; (4) Enhancing nurses' knowledge on NP and strengthening multidisciplinary care coordination.
(1) Implementing comprehensive pain assessments encompassing physical, functional, and psychological impacts; (2) Providing tailored education and training on diverse personalized self-management strategies; (3) Prioritizing psychosocial support and counseling, and facilitating access to resources; (4) Enhancing nurses' knowledge on NP and strengthening multidisciplinary care coordination.
摘要：
Sepsis-associated acute respiratory distress syndrome (ARDS) is a heterogeneous disease with high morbidity and mortality. Lactylation plays a crucial role in sepsis and sepsis-induced lung injury. This study aimed to identify distinct lactylation-based phenotypes in patients with sepsis-associated ARDS and determine relevant molecular biomarkers. We analyzed blood transcriptome and clinical data from patients with sepsis-associated ARDS and calculated the lactylation activity. KEGG pathway analysis, drug sensitivity prediction, and immune cell infiltration analysis were performed. Candidate molecular biomarkers were identified by intersecting the feature genes extracted from four machine learning models. Lactylation activity showed significant heterogeneity among patients with sepsis-associated ARDS, which enabled the classification into low- and high-lactylation activity phenotypes. Patients with high-lactylation experienced longer hospital stays and higher mortality rates, as well as distinct signaling pathways, drug responses, and circulating immune cell abundances. Six key markers (ALDOB, CCT5, EP300, PFKP, PPIA, and SIRT1) were identified to differentiate the two lactylation activity phenotypes, all significantly correlated with circulating immune cell populations. This study revealed significant heterogeneity in lactylation activity phenotypes among patients with sepsis-associated ARDS and identified potential biomarkers to facilitate the application of these phenotypes in clinical practice.
作者：
Luo, Sihuan;Zhao, Xiaomei;Wang, Yijin;Jiang, Miao;Cao, Yi
期刊：
Food and Chemical Toxicology,2025年197:115304 ISSN：0278-6915
通讯作者：
Cao, Y
作者机构：
[Zhao, Xiaomei; Wang, Yijin; Luo, Sihuan; Cao, Yi] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Jiang, Miao] Univ South China, Inst Cardiovasc Dis, Hengyang Med Coll, Key Lab Arteriosclerol Hunan Prov,Hunan Int Sci &, Hengyang 421001, Peoples R China.
通讯机构：
[Cao, Y ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词：
Autophagy;In vivo toxicity;Lipid profiles;Nanoplastics;Oral exposure
摘要：
The wide uses of plastics lead to nanoplastic exposure in reality. Previous studies reported that micro- and nano-plastics (MNPs) disrupted metabolism, but few studies investigated lipid profile changes. Hereby, we exposed mice to vehicles (control), 0.05 or 0.5 mg/kg 20 or 100 nm nanoplastics via gavage, once a day, for 14 days. Albeit no obvious tissue damage, lipidomics data revealed 76 up-regulated and 29 down-regulated lipid molecules in mouse intestines. Further analysis revealed that a number of up-regulated lipid molecules belong to glycerophospholipid (GP). Among GP, we noticed an up-regulation of 9 phosphatidylserine (PS) molecules, and we further verified the presence of autophagosomes and co-localization of typical autophagic lipolysis proteins in intestinal sections, as well as decreased lysosomal associated protein 2 (LAMP2) and increased adipose triglyceride lipase (ATGL) in intestinal homogenates, indicating perturbed autophagic pathway. The exposure also up-regulated 9 phosphatidylinositol (PI) molecules, and we verified a significant decrease of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), indicating altered PI3K-signaling pathway. Besides GP, nanoplastics also significantly up-regulated some sphingolipids (SP), such as ceramide (Cer), and some sterol lipids, such as cholesterol derivatives. Combined, these results suggested that oral exposure to nanoplastics altered lipid profiles and related signaling pathway in mouse intestines.
The wide uses of plastics lead to nanoplastic exposure in reality. Previous studies reported that micro- and nano-plastics (MNPs) disrupted metabolism, but few studies investigated lipid profile changes. Hereby, we exposed mice to vehicles (control), 0.05 or 0.5 mg/kg 20 or 100 nm nanoplastics via gavage, once a day, for 14 days. Albeit no obvious tissue damage, lipidomics data revealed 76 up-regulated and 29 down-regulated lipid molecules in mouse intestines. Further analysis revealed that a number of up-regulated lipid molecules belong to glycerophospholipid (GP). Among GP, we noticed an up-regulation of 9 phosphatidylserine (PS) molecules, and we further verified the presence of autophagosomes and co-localization of typical autophagic lipolysis proteins in intestinal sections, as well as decreased lysosomal associated protein 2 (LAMP2) and increased adipose triglyceride lipase (ATGL) in intestinal homogenates, indicating perturbed autophagic pathway. The exposure also up-regulated 9 phosphatidylinositol (PI) molecules, and we verified a significant decrease of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), indicating altered PI3K-signaling pathway. Besides GP, nanoplastics also significantly up-regulated some sphingolipids (SP), such as ceramide (Cer), and some sterol lipids, such as cholesterol derivatives. Combined, these results suggested that oral exposure to nanoplastics altered lipid profiles and related signaling pathway in mouse intestines.
摘要：
SUMOylation, the covalent attachment of small ubiquitin-like modifier proteins (SUMO) to lysine residues of target substrates, has emerged as a crucial posttranslational modification regulating various cellular processes. Recent studies have revealed that SUMOylation also plays significant roles in host-pathogen interactions during bacterial infections. On the one hand, SUMOylation can modulate host innate immune responses, such as inflammatory signaling and autophagy, to defend against invading bacteria. On the other hand, certain bacterial pathogens have evolved strategies to exploit or manipulate the host SUMOylation machinery to promote their survival and replication. Some bacterial effector proteins directly target host SUMO enzymes or SUMO-conjugated substrates to disrupt host defense mechanisms.Interestingly, a few bacteria have been found to possess their own SUMOylation systems that may contribute to bacterial virulence and stress adaptation. This review summarizes the current understanding of the complex interplay between SUMOylation and bacterial infection, highlighting the dual roles of SUMOylation in host defense and bacterial pathogenesis. We discuss the mechanisms by which SUMOylation regulates host immune responses against bacterial infection and how bacterial pathogens hijack host SUMOylation for their own benefit. Moreover, we explore the potential of targeting SUMOylation as a novel therapeutic strategy for combating bacterial infections. Further research into the intricate relationship between SUMOylation and bacterial infection may provide valuable insights for developing innovative antiinfective therapies.
通讯机构：
[Tan, XF; Yang, QL ; Wu, GL] U;Univ South China, Affiliated Hosp 1, Dept Hepatopancreatobiliary Surg, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Canc Res Inst, Ctr Mol Imaging Probe, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, NHC Key Lab Birth Defect Res & Prevent, Hengyang 421001, Hunan, Peoples R China.;Univ South China, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.
关键词：
NIR-II;Phototheranostics;Endoplasmic reticulum;Triple-negative breast cancer;Intermolecular π–π stacking interaction
摘要：
Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer characterized by an extremely poor prognosis. Photoimmunotherapy has emerged as a promising strategy for the treatment of TNBC. This approach works by selectively destroying tumor cells, releasing tumor-associated antigens, activating the immune system, and effectively inhibiting tumor proliferation and metastasis. However, the majority of current phototheranostic approaches are hindered by limited tissue penetration in the first near-infrared (NIR-I) and ultraviolet-visible (UV-Vis) regions. Additionally, due to the lack of specific subcellular targets, it may be difficult to effectively treat deep-seated lesions with ambiguous and extensive boundaries caused by TNBC metastases. Consequently, the development of effective, deep-penetrating, organelle-targeted phototheranostics is essential for enhancing treatment outcomes in TNBC. This work proposes a novel molecular design strategy of NIR-II phototheranostics to realize planar rigid conjugation and alkyl chain functionalization. The di-hexaalkyl chains in a vertical configuration on the donor (4H-cyclopenta[2,1-b:3,4-b'] dithiophene) and shielding units (fluorene) are introduced to construct a S-D-A-D-S type NIR-II phototheranostics (IR-FCD). The planar and rigid structure of IR-FCD exhibits a robust intramolecular charge transfer capability, a lower band gap, enhanced photon absorption properties, and significant steric hindrance from vertically arranged alkyl chains to minimize non-radiative energy loss. By incorporating N-(but-3-yn-1-yl)-4-methylbenzenesulfonamide at the terminus of an elongated alkyl chain, followed by self-assembly into DSPE-S-S-PEG2000, NIR-II excitable phototheranostics (IR-FCD-Ts NPs) with endoplasmic reticulum (ER) targeting capability were successfully synthesized for imaging-guided photoimmunotherapy of TNBC. The IR-FCD-Ts NPs demonstrate exceptional optical characteristics, with maximum absorption at 1068nm (extending to 1300nm) and emission at 1273nm (extending to 1700nm), along with a high molar absorption coefficient of 2.76*10(4)L/mol·c at 1064nm in aqueous solution. Under exposure to 1064nm laser irradiation, IR-FCD-Ts NPs exhibit superior photothermal properties and have the potential for photodynamic therapy. By targeting ER, thereby inducing ER stress and significantly enhancing immunogenic cell death (ICD) in tumor cells, it triggers a strong antitumor immune response and inhibits the proliferation and metastasis of TNBC.
摘要：
Persistent and maladaptive drug-related memories represent a key component in drug addiction. Converging evidence from both preclinical and clinical studies has demonstrated the potential efficacy of the memory reconsolidation updating procedure (MRUP), a non-pharmacological strategy intertwining two distinct memory processes: reconsolidation and extinction-alternatively termed "the memory retrieval-extinction procedure". This procedure presents a promising approach to attenuate, if not erase, entrenched drug memories and prevent relapse. The present review delineates the applications, molecular underpinnings, and operational boundaries of MRUP in the context of various forms of substance dependence. Furthermore, we critically examine the methodological limitations of MRUP, postulating potential refinement to optimize its therapeutic efficacy. In addition, we also look at the potential integration of MRUP and neurostimulation treatments in the domain of substance addiction. Overall, existing studies underscore the significant potential of MRUP, suggesting that interventions predicated on it could herald a promising avenue to enhance clinical outcomes in substance addiction therapy.
摘要：
Background: The non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) is a recently developed composite indicator of atherogenic lipids. Nevertheless, few studies concern the relationship between NHHR and early-onset post-stroke depression (PSD). Methods: After two weeks of acute ischemic stroke (AIS), early-onset PSD was identified. The Hamilton Depression Scale-17 items (HAMD-17) was used to assess the severity of depression. Patients with HAMD-17 scores ≥7 were divided into an early-onset PSD group. Spearman rank correlation analysis was employed to evaluate the associations between NHHR and HAMD scores across all patients. Logistic regression analysis was conducted to investigate the associations between the NHHR and early-onset PSD. Sensitivity analyses were performed to test the robustness of our findings. Receiver operating characteristic curve (ROC) analysis was used to determine the predictive value of the NHHR for early-onset PSD. Results: Among the 846 patients who were enrolled prospectively, a total of 283 (33.45%) patients were diagnosed with early-onset PSD. The NHHR showed a positive correlation with the HAMD-17 scores (r=0.498, P<0.001). A binary logistic regression model demonstrated that the NHHR (odds ratio [OR], 1.796; 95% confidence interval [CI] 1.452-1.996, P<0.001) was an independent factor for early-onset PSD. The NHHR for early-onset PSD had an area under the curve (AUC) value of 0.798. Conclusions: The findings suggest that the NHHR may be an independent risk factor for early-onset PSD, providing valuable insights for prevention and prognostic management in affected patients.
摘要：
Mycoplasma represents a unique genus of prokaryotic bacteria distinguished by the absence of a cell wall, a characteristic that sets it apart from other bacteria. Within the Mollicutes class, phylogenetic analysis reveals three distinct categories: Spiroplasma, Mycoplasma and Acholeplasma. Mycoplasmas within Pneumoniae are recognized for their capacity to induce a range of diseases in both humans and animals, frequently impacting respiratory and reproductive health. The representative strains in Pneumoniae group, particularly the M. pneumoniae clusters, have garnered significant attention due to their remarkable ability to adhere to, invade, and traverse host cells. This ability is facilitated by specialized structures known as attachment organelles, which possess a unique cytoskeletal structure that supports a distinctive gliding motility mechanism. This mode of motility is distinct from that observed in eukaryotes and the majority of bacteria. The gliding machinery of Mycoplasma is a complex assembly consisting of both surface and internal components, including a terminal button, paired plates, and a structure resembling a bowl or wheel. The internal architecture of the attachment organelles provides the essential scaffold for the operation of this sophisticated motility system. Mycoplasma's gliding motility is crucial for its infection process and its capacity to evade the host immune defenses. Understanding the role of this motility to immune evasion can offer profound insights into the pathogenesis of these bacteria, could pave the way for the development of more effective therapeutic strategies against diseases caused by Mycoplasma and related species.
摘要：
Acute kidney injury (AKI) is associated with poor prognosis. New biomarkers, like neutrophil gelatinase-associated lipocalin (NGAL), are helpful for early warning of AKI. This study aims to investigate the accuracy of NGAL in evaluating the perioperative AKI of liver transplantation. The four databases, PubMed, Web of Science, Embase, and Cochrane Library, were searched for relevant studies published from database inception to August 2023. Results were pooled using random-effects models, and heterogeneity was examined. A total of 16 case-control studies with 1271 patients were included. The results showed that both preoperative [standardized mean difference (SMD) = 0.53; 95% confidence interval (CI): 0.15, 0.91; P < 0.001] and postoperative NGAL levels (SMD = 0.63; 95% CI: 0.24, 1.03; P < 0.001) were higher in the AKI group compared with the non-AKI group. Subgroup analysis by continents showed higher preoperative NGAL levels in AKI patients in the European population (SMD = 1.63; 95% CI: 0.55, 0.27; P = 0.003), but no differences in Asian, African, North American, and South American. Subgroup analysis by continents revealed higher postoperative NGAL levels in the European (SMD = 1.63; 95% CI: 0.55, 0.27; P = 0.002) and Asian populations (SMD = 0.42; 95% CI: 0.04, 0.81; P = 0.039). Higher postoperative NGAL levels in plasma and urine were observed in AKI patients compared with non-AKI patients [plasma (SMD = 1.29; 95% CI: 0.21, 2.38; P = 0.011), urine (SMD = 0.88; 95% CI: 0.18, 1.59; P = 0.035)], while there was no difference in African, North American, South American, and serum NGAL. NGAL level may be an important biomarker for early detection of AKI in the perioperative period of liver transplantation.
摘要：
Outer membrane vesicles (OMVs) derived from Pseudomonas aeruginosa drive inflammation by metabolically reprogramming macrophages to favor aerobic glycolysis. This study shows that OMVs trigger this metabolic shift via Toll-like receptors 2 and 4 (TLR2/4)-dependent activation of the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway. OMV-stimulated macrophages exhibited increased glucose uptake, lactate production, and expression of key glycolytic enzymes, resulting in a higher extracellular acidification rate and a lower oxygen consumption rate. Inhibition of the PI3K/Akt pathway reversed these metabolic changes. Crucially, this metabolic reprogramming was required for OMV-induced secretion of pro-inflammatory cytokines, as inhibition of glycolysis via 2-deoxy-D-glucose treatment attenuated the inflammatory response both in vitro and in vivo. These findings reveal that P. aeruginosa OMVs control metabolism in macrophages through the TLR2/4-PI3K/Akt axis to promote a pro-inflammatory state and identifies glycolysis as a potential therapeutic target for bacteria-associated inflammatory diseases.
期刊：
Tremor and Other Hyperkinetic Movements,2025年15:43 ISSN：2160-8288
通讯作者：
Sun, QY
作者机构：
[Zhang, Hainan; He, Runcheng; Wang, Chunyu] Department of Neurology, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China;[Zhang, Hainan; He, Runcheng; Wang, Chunyu] Clinical Medical Research Center for Stroke Prevention and Treatment of Hunan Province, Department of Neurology, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China;[Li, Mingqiang] Department of Neurology, The First Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, China;[Sun, Qiying; Zhou, Xun; Liu, Lanqing] Department of Geriatric Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, China;[Sun, Qiying] National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
通讯机构：
[Sun, QY ] C;Cent South Univ, Xiangya Hosp, Dept Geriatr Neurol, Changsha 410008, Hunan, Peoples R China.
关键词：
Essential tremor;Essential tremor plus;Medication adherence;Real world study
摘要：
BACKGROUND: Medication adherence in essential tremor (ET) remains poorly characterized. This real world study aimed to investigate adherence rates, clinical correlates, and predictors among ET patients in China. METHODS: A prospective cohort of 318 ET patients (116 pure ET, 202 ET-plus) was followed for a mean of 22.91 ± 3.86 months. Standardized assessments included the Tremor Research Group Essential Tremor Rating Assessment Scale (TETRAS), Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), and Non-Motor Symptoms Scale (NMSS). Adherence was defined as daily use of prescribed tremor medications. Logistic regression identified predictors. RESULTS: Only 27.4% (87/318) maintained daily adherence. ET-plus patients showed higher adherence than pure ET (32.2% vs 19.0%, P = 0.011). Arotinolol was the most common medication. Compared to non-adherent patients, adherent patients showed higher urban residency (P = 0.026), head tremor prevalence (P = 0.002), mild cognitive impairment (P = 0.038), higher TETRAS-I (P = 0.047) and TETRAS-II scores (P = 0.008), as well as lower MoCA scores (P = 0.021). Multivariable analysis showed better medication adherence was significantly associated with higher TETRAS-II score (OR = 1.041, 95% CI = 1.001-1.082, P = 0.047), urban residence (OR = 1.775, 95% CI = 1.066-2.957, P = 0.028), and the presence of head tremor (OR = 1.936, 95% CI = 1.125-3.332, P = 0.017). No significant association was found between ET subtypes and adherence (P > 0.05). CONCLUSION: Medication adherence is alarmingly low in Chinese ET patients, especially in pure ET. Greater tremor severity, presence of head tremor, and urban residence were independently associated with better medication adherence. HIGHLIGHT: Medication adherence among Chinese essential tremor (ET) patients remains suboptimal (only 27.4% in our cohort). ET plus patients showed higher adherence (32.2%) than pure ET (19.0%). Predictors of adherence included severe tremor (TETRAS-II), urban residence, and head tremor. Arotinolol was the predominant treatment. Findings emphasize the need for personalized interventions.
作者机构：
[Tian, Qingzhen; Tang, Zheng; Zhang, Ziyu; Niu, Xiangheng; Li, Shu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Lin, YH; Du, Dan; Niu, Xiangheng; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Zhang, Xiao] Washington State Univ, Sch Chem Engn & Bioengn, Pullman, WA 99164 USA.
通讯机构：
[Lin, YH ; Niu, XH] W;[Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
关键词：
biomarkers;biomedical applications;catalytic signal amplifications;disease diagnosis;nanozymes
摘要：
An overview of nanozyme‐enabled biomedical sensing and diagnosis is presented. The preparation of nanozymes is first summarized, followed by a discussion of typical strategies that are applied to promote the catalytic specificity and activity of nanozymes; whereafter, the main use of nanozymes in biomarker detection and disease diagnosis is discussed; finally, development trends are forecasted, and corresponding challenges are also pointed out. Abstract As nanoscale materials with the function of catalyzing substrates through enzymatic kinetics, nanozymes are regarded as potential alternatives to natural enzymes. Compared to protein‐based enzymes, nanozymes exhibit attractive characteristics of low preparation cost, robust activity, flexible performance adjustment, and versatile functionalization. These advantages endow them with wide use from biochemical sensing and environmental remediation to medical theranostics. Especially in biomedical diagnosis, the feature of catalytic signal amplification provided by nanozymes makes them function as emerging labels for the detection of biomarkers and diseases, with rapid developments observed in recent years. To provide a comprehensive overview of recent progress made in this dynamic field, here an overview of biomedical diagnosis enabled by nanozymes is provided. This review first summarizes the synthesis of nanozyme materials and then discusses the main strategies applied to enhance their catalytic activity and specificity. Subsequently, representative utilization of nanozymes combined with biological elements in disease diagnosis is reviewed, including the detection of biomarkers related to metabolic, cardiovascular, nervous, and digestive diseases as well as cancers. Finally, some development trends in nanozyme‐enabled biomedical diagnosis are highlighted, and corresponding challenges are also pointed out, aiming to inspire future efforts to further advance this promising field.
