作者： Zhou, Shuai;Xiong, Cong;Su, Yinglong;Wang, Yayi;Gao, Yuanyuan;...

期刊： Environmental Pollution,2022年304:119158 ISSN：0269-7491

通讯作者： Duan, Yi(duanyi1987@163.com)

作者机构： [Zhou, Shuai; Duan, Yi] Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reu, Hengyang 421001, Peoples R China.;[Liu, Boyang; Zhou, Shuai; Duan, Yi; Xiong, Cong] Univ South China, Sch Civil Engn, Hengyang 421001, Peoples R China.;[Gao, Yuanyuan; Zhou, Shuai; Tang, Zhenping] Univ South China, Hunan Prov Key Lab Rare Met Minerals Exploitat &, Hengyang 421001, Peoples R China.;[Su, Yinglong] East China Normal Univ, Sch Ecol & Environm Sci, Shanghai 200241, Peoples R China.;[Wang, Yayi] Tongji Univ, Coll Environm Sci & Engn, Shanghai Inst Pollut Control & Ecol Secur, State Key Lab Pollut Control & Resources Reuse, Siping Rd, Shanghai 200092, Peoples R China.

通讯机构： [Yi Duan] H;Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang, 421001, China<&wdkj&>School of Civil Engineering, University of South China, Hengyang, 421001, China

关键词： Antibiotic resistance genes (ARGs);Antibiotic-resistant bacteria (ARB);Distribution;Influencing factor;Uranium mine

摘要： Both heavy metals and radiation could affect the proliferation and dissemination of emerging antibiotic resistance pollutants. As an environmental medium rich in radioactive metals, the profile of antibiotic resistance in uranium mine remains largely unknown. A uranium mine in Guangdong province, China was selected to investigate the distribution and influencing factors of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) including intracellular ARGs (iARGs), adsorbed-extracellular ARGs (aeARGs), and free extracellular ARGs (feARGs). The result indicated that sulfonamide and tetracycline ARB could be generally detected in mining area with the absolute concentrations of 7.70 × 102–5.18 × 105 colony forming unit/g. The abundances of aeARGs in mine soil were significantly higher than those of iARGs (p &lt; 0.05), highlighting the critical contribution of aeARGs to ARGs spread. The feARGs in mine drainage and its receiving river were abundant (3.38 × 104–1.86 × 107 copies/mL). ARB, aeARGs, and iARGs may correlate with nitrogen species and heavy metals (e.g., U and Mn), and feARGs presented a significant correlation with chemical oxygen demand (p &lt; 0.05). These findings demonstrate the occurrence of ARB and ARGs in uranium mine for the first time, thereby contributing to the assessment and control of the ecological risk of antibiotic resistance in radioactive environments. © 2022 Elsevier Ltd

语种： 英文

作者： Guo, Fangyi;Tang, Yanhong;Zhang, Wenjun;Yuan, Hongxia;Xiang, Jing;...

期刊： Applied Microbiology and Biotechnology,2022年106(22):7643-7659 ISSN：0175-7598

通讯作者： Dai, Guozhi(daigz008@126.com);Li, Ranhui(Ranhui81@163.com)

作者机构： [Teng, Wenyou; Yuan, Hongxia; Dai, Guozhi; Guo, Fangyi; Xiang, Jing; Zhang, Wenjun] First Peoples Hosp Chenzhou, Ctr Med Lab, Chenzhou, Peoples R China.;[Lei, Aihua; Guo, Fangyi; Li, Ranhui] Univ South China, Hengyang Med Sch, Pathogen Biol Inst, Hengyang, Peoples R China.;[Dai, Guozhi; Guo, Fangyi; Zhang, Wenjun] Chenzhou Univ South China, Peoples Hosp 1, Ctr Med Lab, Chenzhou, Peoples R China.;[Tang, Yanhong] Guangzhou Med Univ, State Key Lab Resp Dis, Guangzhou, Peoples R China.

通讯机构： [Li, Ranhui] P;[Dai, Guozhi] C;Pathogenic Biology Institute, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Center of Medical Laboratory, The First People’s Hospital of Chenzhou, Chenzhou, China<&wdkj&>Center of Medical Laboratory, Affiliated the First People’s Hospital of Chenzhou of University of South China, Chenzhou, China

关键词： Dendritic cells;DnaJ,;Heat shock proteins,;Recombinant vaccine,;Ureaplasma urealyticum,

摘要： Abstract: Ureaplasma urealyticum (U. urealyticum, Uu) is a common sexually transmitted pathogen that is responsible for diseases such as non-gonococcal urethritis, chorioamnionitis, and neonatal respiratory diseases. The rapid emergence of multidrug-resistant bacteria threatens the effective treatment of Uu infections. Considering this, vaccination could be an efficacious medical intervention to prevent Uu infection and disease. As a highly conserved molecular chaperone, DnaJ is expressed and upregulated by pathogens soon after infection. Here, we assessed the vaccine potential of recombinant Uu-DnaJ in a mouse model and dendritic cells. Results showed that intramuscular administration of DnaJ induced robust humoral- and T helper (Th) 1 cell-mediated immune responses and protected against genital tract infection, inflammation, and the pathologic sequelae after Uu infection. Importantly, the DnaJ protein also induced the maturation of mouse bone marrow–derived dendritic cells (BMDCs), ultimately promoting naïve T cell differentiation toward the Th1 phenotype. In addition, adoptive immunization of DnaJ-pulsed BMDCs elicited antigen-specific Immunoglobulin G2 (IgG2) antibodies as well as a Th1-biased cellular response in mice. These results support DnaJ as a promising vaccine candidate to control Uu infections. Key points: • A novel recombinant vaccine was constructed against U. urealyticum infection. • Antigen-specific humoral and cellular immune responses after DnaJ vaccination. • Dendritic cells are activated by Uu-DnaJ, which results in a Th1-biased immune response. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Tan, Shaowen;Xu, Zili

期刊： Computational and Mathematical Methods in Medicine,2022年2022 ISSN：1748-6718

通讯作者： Xu, ZL

作者机构： [Tan, Shaowen; Xu, Zili] Univ South China, Hengyang Med Sch, Affiliated Hosp 3, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Xu, ZL ] U;Univ South China, Hengyang Med Sch, Affiliated Hosp 3, Dept Cardiovasc Med, Hengyang 421001, Hunan, Peoples R China.

摘要： In this study, dictionary learning and expectation maximization reconstruction (DLEM) was combined to denoise 64-slice spiral CT images, and results of coronary angiography (CAG) were used as standard to evaluate its clinical value in diagnosing coronary artery diseases. 120 patients with coronary heart disease (CHD) confirmed by CAG examination were retrospectively selected as the research subjects. According to the random number table method, the patients were divided into two groups: the control group was diagnosed by conventional 64-slice spiral CT images, and the observation group was diagnosed by 64-slice spiral CT images based on the DLEM algorithm, with 60 cases in both groups. With CAG examination results as the standard, the diagnostic effects of the two CT examination methods were compared. The results showed that when the number of iterations of maximum likelihood expectation maximization (MLEM) algorithm reached 50, the root mean square error (RMSE) and peak signal to noise ratio (PSNR) values were similar to the results obtained by the DLEM algorithm under a number of iterations of 10 when the RMSE and PSNR values were 18.9121 dB and 74.9911 dB, respectively. In the observation group, 28.33% (17/60) images were of grade 4 or above before processing; after processing, it was 70% (42/60), significantly higher than the proportion of high image quality before processing. The overall diagnostic consistency, sensitivity, specificity, and accuracy (88.33%, 86.67%, 80%, and 85%) of the observation group were better than those in the control group (60.46%, 62.5%, 58.33%, and 61.66%). In conclusion, the DLEM algorithm has good denoising effect on 64-slice spiral CT images, which significantly improves the accuracy in the diagnosis of coronary artery stenosis and has good clinical diagnostic value and is worth promoting. © 2022 Shaowen Tan and Zili Xu.

语种： 英文

作者： Shen, Caijie;Wang, Jian;Feng, Mingjun;Peng, Jianye;Du, Xiangfeng;...

期刊： Cardiovascular Engineering and Technology,2022年13(5):651-661 ISSN：1869-408X

通讯作者： Chu, Huimin;Chen, Xiaomin

作者机构： [Wang, Jian; Chu, Huimin; Chen, XM; Chen, Xiaomin; Du, Xiangfeng; Shen, Caijie; Feng, Mingjun] Ningbo First Hosp, Dept Cardiovasc Med, 59 Liuting St, Ningbo 315000, Zhejiang, Peoples R China.;[Peng, Jianye] Univ South China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 315000, Hunan, Peoples R China.

通讯机构： [Chu, HM; Chen, XM] N;Ningbo First Hosp, Dept Cardiovasc Med, 59 Liuting St, Ningbo 315000, Zhejiang, Peoples R China.

关键词： H2O2;Inflammatory status;NF-κB;Nrf2/ARE;Oxidative stress;The mitochondrial-derived peptide MOTS-c

摘要： Aim: Oxidative stress and the inflammatory response contribute to the progression of cardiovascular disease. The present study aimed to investigate whether the mitochondrial-derived peptide MOTS-c could alleviate H2O2-induced oxidative stress and inflammatory status in H9c2 cells through activation of nuclear factor erythroid 2-related Factor 2 (Nrf2)/antioxidative response element (ARE) and inhibition of the NF-κB pathway. Methods: Rat H9c2 cardiomyocytes were obtained, and 10, 20 or 50 μM MOTS-c was pretreated for 24 h before treatment with H2O2. Then, the cell was treated with 100 μM H2O2 for 1 h to induce oxidative stress. An inhibition model of sh-Nrf2 was constructed via a lentivirus expression system, and an activation model of NF-κB was achieved using phorbol 12-myristate-13-acetate(PMA). Cell viability was determined using a Cell Counting kit-8 assay. Relative measurement of relative protein and mRNA expression used western blotting and qRT-PCR, respectively. Intracellular reactive oxygen species (ROS) levels were detected using dichlorodihydrofluorescein diacetate, and malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined via commercial kits. The protein expression and distribution in the cells were visualized by immunofluorescence analysis. Enzyme-linked immunosorbent assay was used to detect the levels of inflammatory cytokines, including TNF-α, IL-6 and IL-1β. Results: We found that H2O2 treatment significantly decreased cell viability and the level of SOD, increased the levels of ROS and MDA, and upregulated the expression of inflammatory cytokines, including TNF-α, IL-6 and IL-1β, in H9c2 cells. The expression levels of Nrf2, HO-1 and NQO-1 were significantly downregulated in the H2O2, while the phosphorylation of NF-κBp65 was promoted by H2O2. However, pretreatment with MOTS-c significantly reversed H2O2-induced damage in H9c2 cells. Moreover, both inhibition of the Nrf2/ARE pathway and activation of the NF-κB pathway significantly decreased the effects of MOTS-c, suggesting that MOTS-c might play a role in alleviating oxidative damage via the Nrf2/ARE and NF-κB pathways. Conclusions: Our investigation indicated that MOTS-c could protect against H2O2-induced inflammation and oxidative stress in H9c2 cells by inhibiting NF-κB and activating the Nrf2/ARE pathways. © 2021, Biomedical Engineering Society.

语种： 英文

作者： Hou, Liangjuan;Zeng, Xin;Li, Xuan;Zhao, Chune;Zou, Juan;...

期刊： Computational and Mathematical Methods in Medicine,2022年2022 ISSN：1748-6718

通讯作者： Liu, Gang(liugangscience@163.com)

作者机构： [Hou, Liangjuan; Zeng, Xin] Department of Histology and Embryology, Chongqing Three Gorges Medical College, Wanzhou, Chongqing;404120, China;[Li, Xuan] Department of Embryo Laboratory, Changsha Reproductive Medical Hospital, Hunan, Changsha;410000, China;[Zhao, Chune; Liu, Gang] Department of Infectious Disease, Three Gorges Hospital of Chongqing University, Chongqing University, Wanzhou, Chongqing

通讯机构： [Liu, G.] D;Department of Infectious Disease, Three Gorges Hospital of Chongqing University, Chongqing University, Wanzhou, Chongqing, China

摘要： Objective. To evaluate the expression profile of MCM6 in HCC and the relationship between MCM6 level and clinicopathological parameters through bioinformatics analysis of several databases. Methods. MCM expression level, clinical parameters, survival data, and gene set enrichment analysis were analyzed by bioinformatics database, including Oncomine™, UALCAN, HCCDB, TCGA, cBioPortal, and LinkedOmics. Real-time PCR, western blotting, and IHC staining were conducted to identify the expression of MCM6 in HCC compared to normal liver tissues. Results. Bioinformatics analysis indicated that the mRNA of MCM6 was obviously increased in multiple cancer types, especially in HCC. MCM6 level was positively associated with multiple clinical parameters (stage 3 and grades 3 and 4) and negatively associated with patient outcomes (overall survival). Moreover, enrichment of functions and signaling pathways analysis of MCM6 suggested that MCM6 might mediate DNA replication and cellular metabolism to promote the development and progression of HCC. Furthermore, IHC staining and western blotting indicated that the MCM6 was enhanced in HCC tissue, and MCM6 could promote HCC proliferation in activating Notch pathway via WB and bioinformatic analysis. Conclusion. This study actually revealed the expression and related functions of MCM6 in HCC. Furthermore, MCM6 is a carcinogenic role in activating Notch pathway to promote HCC cell proliferation, which may be a new prognostic biomarker and therapeutic target for HCC patients. © 2022 Liangjuan Hou et al.

语种： 英文

作者： Chen, Lei;Liu, Jinquan;Cao, Chen;Tang, Shuangyang;Lv, Changyin;...

期刊： Analytica Chimica Acta,2021年1184:338986 ISSN：0003-2670

通讯作者： Li, Le

作者机构： [Yang, Shengyuan; Liu, Ling; Liu, Jinquan; Li, Le; Chen, Lei; Lv, Changyin; Xiao, Xilin; Sun, Lin; Zhu, Bingyu] Univ South China, Hengyang Med Sch, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Yang, Shengyuan; Liu, Ling; Liu, Jinquan; Li, Le; Chen, Lei; Lv, Changyin; Xiao, Xilin; Sun, Lin; Zhu, Bingyu] Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Hunan, Peoples R China.;[Yang, Shengyuan; Liu, Ling; Liu, Jinquan; Li, Le; Chen, Lei; Lv, Changyin; Xiao, Xilin; Sun, Lin; Zhu, Bingyu] Univ South China, Key Lab Hlth Hazard Factors Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;[Cao, Chen] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Tang, Shuangyang] Univ South China, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Li, Le] U;Univ South China, Hengyang Med Sch, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.

关键词： Electrochemical sensing;Gold nanoparticles (AuNPs);Hybrid chain reaction (HCR);Silver nanoclusters (AgNCs);Uranyl ions (UO(2)(2+))

摘要： Herein, a dual-signal amplification electrochemical sensing has been proposed for the ultrasensitive detection of uranyl ions (UO22+) by integration of gold nanoparticles (AuNPs) and hybridization chain reaction (HCR)-assisted synthesis of silver nanoclusters (AgNCs). In this sensing platform, AuNPs are used as an ideal signal amplification carrier, aiming at increasing the loads of UO22+-specific DNAzyme on the gold electrode. In the presence of UO22+, UO22+-specific DNAzyme can be activated, leading to the cleavage of substrate strands (S-DNA). Then, HCR is triggered to produce long dsDNA through hybridization the probe with the ssDNA on the electrode surface. As a result, an amplified electrochemical response can be detected by inserting a large amount of AgNCs generated in situ using dsDNA as template. Featured with amplification efficiency, good specificity and high sensitivity, the strategy could quantitatively detect UO22+ down to 6.2 pM with a linear calibration range from 20 pM to 5000 pM. The proposed sensing platform has been also successfully demonstrated the practical application of detecting UO22+, indicating that the developed method has the potential applications and can open up a new avenue for highly sensitive detection of UO22+ in environmental monitoring. © 2021 Elsevier B.V.

语种： 英文

作者： Cao, Ting;Peng, Binfeng;Zhou, Xiangping;Cai, Jialun;Tang, Yun;...

期刊： Applied Microbiology and Biotechnology,2021年105(12):4805-4818 ISSN：0175-7598

通讯作者： Liu, Shuangquan(dantelliu@163.com)

作者机构： [Liu, Shuangquan; Luo, Jie; Peng, Binfeng; Cai, Jialun; Zhang, Ji; Xie, Haitao; Tang, Yun; Cao, Ting; Zhou, Xiangping] Univ South China, Dept Clin Lab, Affiliated Hosp 1, Hengyang 421000, Hunan, Peoples R China.

通讯机构： [Liu, Shuangquan] D;Department of Clinical Laboratory, The First Affiliated Hospital of University of South China, Hengyang, 421000, Hunan, China.

关键词： Endoplasmic reticulum;ER stress;ERAD;Autophagy;HIF-1;Mitochondrial biogenesis

摘要： Abstract: The endoplasmic reticulum (ER) is a multifunctional organelle, which is crucial for correct folding and assembly of secretory and transmembrane proteins. Perturbations of ER function can cause ER stress. ER stress can activate the unfolded protein response (UPR) to cope with the accumulation of misfolded proteins and protein toxicity. UPR is a coordination system that regulates transcription and translation, leading to the recovery of ER homeostasis or cell death. However, cells have an integrated signaling system to cope with ER stress, which helps cells to restore and balance their ER function. The main components of this system are ER-associated degradation (ERAD), autophagy, hypoxia signaling, and mitochondrial biogenesis. If the balance cannot be restored, the imbalance will lead to cell death or apoptosis, or even to a series of diseases. In this review, a series of activities to restore the homeostasis of cells during ER stress are discussed. Key points: • Endoplasmic reticulum (ER) plays a key role in the biological process of cells. • Perturbations of ER function can cause ER stress, including the ER overload response (EOR), sterol-regulated cascade reaction, and the UPR. • Cells have an integrated signaling system (ERAD, autophagy, hypoxia signaling, and mitochondrial biogenesis) to cope with the adverse impact caused by ER stress. Graphical abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Li, Bin*;Liu, Guoping

期刊： FRONTIERS IN NEUROROBOTICS,2021年15:128 ISSN：1662-5218

通讯作者： Li, Bin

作者机构： [Li, Bin] South China Univ, Dept Neurol, Affiliated Hosp 3, Hengyang, Peoples R China.;[Liu, Guoping] South China Univ, Dept Gastroenterol, Affiliated Hosp 3, Hengyang, Peoples R China.

通讯机构： [Li, Bin] S;South China Univ, Dept Neurol, Affiliated Hosp 3, Hengyang, Peoples R China.

关键词： Artificial intelligence neural network;Convolutional Neural Network;Magnetic resonance;image segmentation;Butyphthalide;Edaravone;Acute cerebral infarction;Neural function

摘要： This research was developed to investigate the effect of artificial intelligence neural network-based magnetic resonance imaging (MRI) image segmentation on the neurological function of patients with acute cerebral infarction treated with butylphthalide combined with edaravone. Eighty patients with acute cerebral infarction were selected as the research subjects, and the MRI images of patients with acute cerebral infarction were segmented by convolutional neural networks (CNN) upgraded algorithm model. MRI images of patients before and after treatment of butylphthalide combined with edaravone were compared to comprehensively evaluate the efficacy of this treatment. The results showed that compared with the traditional CNN algorithm, the running time of the CNN upgraded algorithm adopted in this study was significantly shorter, and the Loss value was lower than that of the traditional CNN model. Upgraded CNN model can realize accurate segmentation of cerebral infarction lesions in MRI images of patients. In addition, the degree of cerebral infarction and the degree of arterial stenosis were significantly improved after treatment with butylphthalide and edaravone. Compared with that before treatment, the number of patients with severe cerebral infarction or even vascular stenosis decreased significantly (P < 0.05), and gradually changed to mild vascular stenosis, and the neurological dysfunction of patients was also significantly improved. In short, MRI image segmentation based on artificial intelligence neural network can well-evaluate the efficacy and neurological impairment of butylphthalide combined with edaravone in the treatment of acute cerebral infarction, and it was worthy of promotion in clinical evaluation of the treatment effect of acute cerebral infarction. © Copyright © 2021 Li and Liu.

语种： 英文

作者： Zeng, Junfa;Tao, Jun;Xia, Linzhen;Zeng, Zhaolin;Chen, Jiaojiao;...

期刊： BIOCHEMISTRY AND CELL BIOLOGY,2021年99(3):339-347 ISSN：0829-8211

通讯作者： Wang, Zuo(smt121101@163.com)

作者机构： [Chen, Jiaojiao; Tao, Jun; Liu, Lushan; Xia, Linzhen; Wang, Zuo; Zeng, Junfa] Univ South China, Hengyang Med Coll, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov,Hunan Int Sci &, Hengyang 421001, Hunan, Peoples R China.;[Zeng, Junfa] Univ South China, Affiliated Hosp 2, Hengyang, Peoples R China.;[Zeng, Zhaolin] Chongqing Med Univ, Peoples Hosp Nanchuan, Dept Cardiol, Chongqing 408499, Peoples R China.;[Meng, Jun] Univ South China, Funct Dept, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Wang, Z.] I;Institute of Cardiovascular Disease, China

关键词： atherosclerosis;melatonin;NLRP3;UQCRC1;ROS;athérosclérose;mélatonine;NLRP3;UQCRC1;DRO

摘要： Atherosclerosis (AS) is a chronic inflammatory disease that involves cell death and endothelial dysfunction. Melatonin is an endocrine hormone with anti-inflammatory and anti-AS effects. However, the underlying molecular mechanisms for the anti-AS effects of melatonin are unknown. A previous study has shown that pyroptosis plays a detrimental role in the development of AS, therefore, this study was designed to investigate the anti-pyroptotic effects and potential mechanisms of melatonin in atherosclerotic endothelium. Our results show that melatonin attenuated the expression of genes related to pyroptosis, including NLRP3, caspase-1, and IL-1b, in human umbilical vein endothelial cells treated with oxidized low-density lipoprotein. Furthermore, melatonin up-regulated the expression of ten-eleven translocation 2 (TET2), inhibited the methylation of ubiquinol-cytochrome c reductase core protein 1 (UQCRC1), and reduced pyroptosis. The upregulation of UQCRC1 by melatonin improved mitochondrial function, thereby inhibiting oxidative stress and endothelial cell pyroptosis. Collectively, our results indicate that melatonin prevents endothelial cell pyroptosis by up-regulating TET2 to inhibit the methylation of UQCRC1 and improving mitochondrial function. © 2021, Canadian Science Publishing. All rights reserved.

语种： 英文

作者： Liu, Jin;Liang, Long;Li, Xin;Peng, Yuan liang;Zhang, Ji;...

期刊： Biotechnology Letters,2021年43(6):1131-1142 ISSN：0141-5492

通讯作者： Nie, Ling

作者机构： [Liu, Jing; Liu, Jin; Peng, Yuan liang; Li, Xin] Cent South Univ, Sch Life Sci, Mol Biol Res Ctr, Hunan Prov Key Lab Basic & Appl Hematol, Changsha 410078, Hunan, Peoples R China.;[Nie, Ling; Liang, Long] Cent South Univ, Xiangya Hosp, 87 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.;[Zhang, Ji] South China Univ, Dept Clin Lab, Affiliated Hosp 1, Hengyang 421000, Hunan, Peoples R China.;[Wang, Xiao long] Cent South Univ, Sch Life Sci, Changsha 410078, Hunan, Peoples R China.

通讯机构： [Nie, Ling] C;Cent South Univ, Xiangya Hosp, 87 Xiangya Rd, Changsha 410078, Hunan, Peoples R China.

关键词： AICAR;AMPK;Decitabine;DNA damage;Myelodysplastic syndromes

摘要： To investigate the efficacy and safety of the AMPK activator AICAR alone or in combination with decitabine on myelodysplastic syndromes (MDS). p-AMPK (Thr172) expression was lower in MDS samples than in healthy donors. AMPK agonist AICAR inhibited the proliferation of MDS cell lines (SKM1 and MDS-L) (P < 0.05). The results from flow cytometry suggested that AICAR induced G0/G1 phase arrest and apoptosis through inducing DNA damage, as confirmed by immunofluorescence analysis in MDS cell lines. AICAR alone or in combination with decitabine was applied to the two MDS cell lines, and the combination index values at all concentrations were significantly < 1. This strong synergistic effect was also corroborated in the primary MDS patient samples and in an MDS cell line xenograft mouse model. Furthermore, immunohistochemical staining showed that there was more DNA damage accumulation in the combination group than that in any other groups. This is the first report on how the AICAR suppresses MDS cell proliferation and synergizes with decitabine via DNA damage induction. AICAR in combination with decitabine may be a promising therapeutic strategy in MDS.

语种： 英文

作者： Li Guoping;Jin Xiaosheng;Zheng, Jisheng;Jiang Na;Shi Wang*

期刊： Biotechnology and Applied Biochemistry,2021年68(1):165-172 ISSN：0885-4513

通讯作者： Shi Wang

作者机构： [Jiang Na; Jin Xiaosheng; Zheng, Jisheng; Li Guoping] Tongde Hosp Zhejiang Prov, Dept Resp Med, Hangzhou, Zhejiang, Peoples R China.;[Shi Wang] Univ South China, Affiliated Nanhua Hosp, Dept Resp Med, Hengyang, Hunan, Peoples R China.

通讯机构： [Shi Wang] U;Univ South China, Affiliated Nanhua Hosp, Dept Resp Med, Hengyang, Hunan, Peoples R China.

关键词： non-small cell lung cancer;ubiquitin c-terminal hydrolase-l3;proliferation;cell cycle;apoptosis

摘要： Ubiquitin C-terminal hydrolase-L3 (UCH-L3) is a deubiquitinase that has a crucial role in oncogenesis. This study was aimed to explore the biological function of UCH-L3 in non-small cell lung cancer (NSCLC). Bioinformatics analysis was used to detect UCH-L3 expression in NSCLC tissues and normal lung tissues, and to analyze the relationship between UCH-L3 expression and survival of patients. qRT-PCR and western blotting assays were used to detect UCH-L3 expression in NSCLC tumor tissues and adjacent normal tissues. CCK-8 assay was performed to examine the effect of UCH-L3 on NSCLC cell proliferation. Flow cytometry assay was conducted to examine the effect of UCH-L3 on NSCLC cell cycle and apoptosis. The expression of UCH-L3 in NSCLC tissues was markedly higher than in normal lung tissues, and high expression of UCH-L3 was positively associated with the poor survival of patients. UCH-L3 knockdown significantly inhibited the proliferation of NSCLC cells, whereas UCH-L3 overexpression had the opposite effect. Moreover, UCH-L3 promoted NSCLC cells proliferation via accelerating cell cycle and inhibiting cell apoptosis. UCH-L3 is upregulated in NSCLC and positively associated with the poor survival, and its expression contributes to NSCLC cell proliferation by accelerating cell cycle and inhibiting cell apoptosis. © 2020 International Union of Biochemistry and Molecular Biology, Inc.

语种： 英文

作者： 唐振平;肖莎莎;段毅;刘迎九;高媛媛;...

期刊： 环境科学,2021年42(7):3375-3384 ISSN：0250-3301

通讯作者： Zhou, Shuai(zs402606665@126.com)

作者机构： [唐振平; 段毅; 刘迎九; 周帅] Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang;421001, China;Hunan Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, Hengyang;[肖莎莎; 高媛媛] School of Civil Engineering, University of South China, Hengyang;[吴月月; 陈怡雯] Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang

关键词： 石化废水;耐药菌(ARB);耐药基因(ARGs);分布;去除;影响因子

摘要： 抗生素耐药性污染已成为全球新兴环境问题之一.本研究选取某座石化废水处理厂,对耐药菌(ARB)和3种形态耐药基因(ARGs):细胞内耐药基因(iARGs) 、细胞外附着态耐药基因(aeARGs)和游离态耐药基因(feARGs)的分布特征与去除效能开展研究.结果表明,废水处理厂中检出四环素、磺胺和氨苄西林这3类ARB,其绝对浓度为8.45×10~2 ~ 2.38×10~5CFU·mL~(-1).厌氧处理可使这3类ARB绝对浓度下降0.04 lg ~ 0.21 lg;曝气和沉淀处理对ARB的影响因其类型而异;出水ARB绝对浓度高出进水水平0.12 lg ~ 0.63 lg.活性污泥中aeARGs和iARGs绝对丰度分别为1.96×10~7 ~ 3.02×10~(10) copies·g~(-1)和5.22×10~7 ~ 4.15×10~(10) copies·g~(-1);而废水中feARGs绝对丰度为5.90×10~8 ~ 1.01×10~(12) copies·L~(-1).厌氧处理可去除0.13 lg ~ 0.65 lg aeARGs和0.04 lg ~ 0.28 lg iARGs;曝气和沉淀处理对aeARGs和iARGs的去除效果受ARGs类型和形态影响;出水中feARGs绝对丰度较进水升高0.06 lg ~ 0.81 lg.冗余分析表明,ARB浓度与COD、Cl~-和总氮浓度显著正相关(P < 0.05); aeARGs丰度与COD和总氮浓度显著正相关(P < 0.05); iARGs和feARGs丰度均与重金属浓度显著正相关(P < 0.05).本研究证实了石化废水处理厂具有ARB和不同形态ARGs的富集风险,并为特种工业废水耐药性污染研究与防治提供理论基础.

语种： 中文

作者： Li, Wei;Zhou, Xiangping;Cai, Jialun;Zhao, Feijun;Cao, Ting;...

期刊： Applied Microbiology and Biotechnology,2021年105(1):353-366 ISSN：0175-7598

通讯作者： Liu, Shuangquan;Xiao, Xinhua

作者机构： [Liu, Shuangquan; Cai, Jialun; Li, Wei; Luo, Chunyi; Cao, Ting; Ning, Lichang; Zhou, Xiangping] Univ South, Affiliated Hosp 1, Dept Clin Lab, 69 Chuanshan Rd, Hengyang City 421000, Hunan, Peoples R China.;[Zhao, Feijun] Univ South China, Inst Pathogen Biol, Hengyang, Hunan, Peoples R China.;[Zhao, Feijun] Univ South China, Key Lab Special Pathogen Prevent & Control Hunan, Hengyang, Hunan, Peoples R China.;[Xiao, Xinhua] Univ South China, Dept Endocrinol, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.

通讯机构： [Liu, Shuangquan; Xiao, Xinhua] U;Univ South, Affiliated Hosp 1, Dept Clin Lab, 69 Chuanshan Rd, Hengyang City 421000, Hunan, Peoples R China.;Univ South China, Dept Endocrinol, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.

关键词： Endoplasmic reticulum stress;Inflammation;Macrophages;ROS/NF-κB pathway;Syphilis;Treponema pallidum

摘要： In response to danger signals, macrophages rapidly produce many inflammatory cytokines that trigger the cascade release of inflammatory mediators, leading to tissue damage, which is an important cause of clinical manifestations of syphilis at all stages. However, we still know very little about the specific mechanism of this process. Tp0768 is an infection-stage–dependent antigen that plays an important role in the infection of Treponema pallidum. In this study, we demonstrated that Tp0768 stimulation of macrophages can cause IL-1β, IL-6, and IL-8 mRNA expression levels to increase in a dose- and time-dependent manner. Further research showed that Tp0768 activated ER stress and the ROS/NF-κB pathway in macrophages. Inhibition of ER stress and the ROS/NF-κB pathway inhibited the expression of IL-1β, IL-6, and IL-8 induced by Tp0768. In addition, pretreatment with a PERK pathway inhibitor significantly reduced the expression of the NF-κB and JNK pathways, while also downregulating the expression of IL-1β, IL-6, and IL-8. Tp0768 stimulation can activate IRE1α/XBP-1 signaling and participate in the induction of inflammatory cytokines through the JNK pathway. These findings indicate that Tp0768 promotes the secretion of proinflammatory cytokines IL-1β, IL-6, and IL-8 by macrophages through ER stress and the ROS/NF-κB pathway, which are also involved in the activation of the NF-κB and JNK pathways that are induced by the PERK pathway and activation of IRE1α/XBP-1 signaling. • This study found for the first time that the recombinant Treponema pallidum protein Tp0768 promotes the production of IL-1β, IL-6, and IL-8 by macrophages through ER stress. • Recombinant Treponema pallidum protein Tp0768 regulates the ROS/NF-κB pathway through ER stress. • ER stress-related pathway PERK induces the expression of IL-1β, IL-6, and IL-8 by activating the NF-κB pathway and the JNK pathway. • IRE1α can induce the splicing of XBP-1mRNA and activate the JNK pathway.

语种： 英文

作者： Li, Songjiao;Cheng, Dan;He, Longwei*;Yuan, Lin

期刊： FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2021年9:768698 ISSN：2296-4185

通讯作者： He, Longwei

作者机构： [He, Longwei; Li, Songjiao] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Dept Pharm & Pharmacol, Canc Res Inst,Hengyang Med Sch,Affiliated Hosp 1, Hengyang, Peoples R China.;[Cheng, Dan] Univ South China, Hengyang Med Sch, Affiliated Nanhua Hosp, Hengyang, Peoples R China.;[Yuan, Lin; Cheng, Dan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha, Peoples R China.

通讯机构： [He, Longwei] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Dept Pharm & Pharmacol, Canc Res Inst,Hengyang Med Sch,Affiliated Hosp 1, Hengyang, Peoples R China.

关键词： surgical navigation;NIR-I;NIR-II;tumor;Fluorescence Imaging;Image-guided cancer surgeries

摘要： Cancer is still one of the main causes of morbidity and death rate around the world, although diagnostic and therapeutic technologies are used to advance human disease treatment. Currently, surgical resection of solid tumors is the most effective and a prior remedial measure to treat cancer. Although medical treatment, technology, and science have advanced significantly, it is challenging to completely treat this lethal disease. Near-infrared (NIR) fluorescence, including the first near-infrared region (NIR-I, 650–900nm) and the second near-infrared region (NIR-II, 1,000–1,700nm), plays an important role in image-guided cancer surgeries due to its inherent advantages, such as great tissue penetration, minimal tissue absorption and emission light scattering, and low autofluorescence. By virtue of its high precision in identifying tumor tissue margins, there are growing number of NIR fluorescence-guided surgeries for various living animal models as well as patients in clinical therapy. Herein, this review introduces the basic construction and operation principles of fluorescence molecular imaging technology, and the representative application of NIR-I/II image-guided surgery in biomedical research studies are summarized. Ultimately, we discuss the present challenges and future perspectives in the field of fluorescence imaging for surgical navigation and also put forward our opinions on how to improve the efficiency of the surgical treatment. Copyright © 2021 Li, Cheng, He and Yuan.

语种： 英文

作者： Xiao, Yubin;Xiao, Zheng;Feng, Xiang;Chen, Zhiping;Kuang, Linai;...

期刊： BMC Bioinformatics,2020年21(1):1-22 ISSN：1471-2105

通讯作者： Wang, Lei

作者机构： [Feng, Xiang; Chen, Zhiping; Wang, Lei; Xiao, Yubin] Changsha Univ, Coll Comp Engn & Appl Math, Changsha 410001, Peoples R China.;[Xiao, Zheng] Univ South China, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Hunan, Peoples R China.;[Kuang, Linai; Wang, Lei; Xiao, Yubin] Xiangtan Univ, Key Lab Hunan Prov Internet Things & Informat Sec, Xiangtan 411105, Peoples R China.

通讯机构： [Wang, Lei] C;[Wang, Lei] X;Changsha Univ, Coll Comp Engn & Appl Math, Changsha 410001, Peoples R China.;Xiangtan Univ, Key Lab Hunan Prov Internet Things & Informat Sec, Xiangtan 411105, Peoples R China.

关键词： LncRNA-disease association prediction;Features;Random walk;Multiple linear regression;Artificial neural network

摘要： Background: Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) are closely associated with human diseases, and it is useful for the diagnosis and treatment of diseases to get the relationships between lncRNAs and diseases. Due to the high costs and time complexity of traditional bio-experiments, in recent years, more and more computational methods have been proposed by researchers to infer potential lncRNA-disease associations. However, there exist all kinds of limitations in these state-of-the-art prediction methods as well. Results: In this manuscript, a novel computational model named FVTLDA is proposed to infer potential lncRNA-disease associations. In FVTLDA, its major novelty lies in the integration of direct and indirect features related to lncRNA-disease associations such as the feature vectors of lncRNA-disease pairs and their corresponding association probability fractions, which guarantees that FVTLDA can be utilized to predict diseases without known related-lncRNAs and lncRNAs without known related-diseases. Moreover, FVTLDA neither relies solely on known lncRNA-disease nor requires any negative samples, which guarantee that it can infer potential lncRNA-disease associations more equitably and effectively than traditional state-of-the-art prediction methods. Additionally, to avoid the limitations of single model prediction techniques, we combine FVTLDA with the Multiple Linear Regression (MLR) and the Artificial Neural Network (ANN) for data analysis respectively. Simulation experiment results show that FVTLDA with MLR can achieve reliable AUCs of 0.8909, 0.8936 and 0.8970 in 5-Fold Cross Validation (fivefold CV), 10-Fold Cross Validation (tenfold CV) and Leave-One-Out Cross Validation (LOOCV), separately, while FVTLDA with ANN can achieve reliable AUCs of 0.8766, 0.8830 and 0.8807 in fivefold CV, tenfold CV, and LOOCV respectively. Furthermore, in case studies of gastric cancer, leukemia and lung cancer, experiment results show that there are 8, 8 and 8 out of top 10 candidate lncRNAs predicted by FVTLDA with MLR, and 8, 7 and 8 out of top 10 candidate lncRNAs predicted by FVTLDA with ANN, having been verified by recent literature. Comparing with the representative prediction model of KATZLDA, comparison results illustrate that FVTLDA with MLR and FVTLDA with ANN can achieve the average case study contrast scores of 0.8429 and 0.8515 respectively, which are both notably higher than the average case study contrast score of 0.6375 achieved by KATZLDA. Conclusion: The simulation results show that FVTLDA has good prediction performance, which is a good supplement to future bioinformatics research. © 2020, The Author(s).

语种： 英文

作者： Shi, Lisha;Chen, Li;Wan, Yanjian*;Zeng, Huaicai;Xia, Wei*

期刊： Chemosphere,2020年251(Jul.):126385 ISSN：0045-6535

通讯作者： Wan, Yanjian;Xia, Wei

作者机构： [Xia, Wei; Shi, Lisha; Chen, Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Key Lab Environm & Hlth HUST,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Shi, Lisha; Chen, Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Minist Environm Protect, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Shi, Lisha; Chen, Li] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, State Key Lab Environm Hlth Incubat, Wuhan 430030, Hubei, Peoples R China.;[Wan, Yanjian] Wuhan Ctr Dis Prevent & Control, Inst Environm Hlth, Wuhan 430015, Hubei, Peoples R China.;[Zeng, Huaicai] Univ South China, Sch Publ Hlth, Dept Prevent Med, 28 Western Changsheng Rd, Hengyang 421001, Peoples R China.

通讯机构： [Wan, Yanjian] W;[Xia, Wei] H;Wuhan Ctr Dis Prevent & Control, Inst Environm Hlth, Wuhan 430015, Hubei, Peoples R China.;Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Wuhan 430030, Peoples R China.

关键词： Fipronil;Insecticide;Yangtze river;Contamination;Removal

摘要： Fipronil (FP) is an emerging insecticide, however, its occurrence in drinking water and source water is limited. In this work, a total of 789 tap water and 95 ground water samples were collected from China in June 2019 in order to assess occurrence of FP and its derivatives (FPs). FPs were also analyzed in source, treated (n = 10, July), and tap water samples (n = 81, July and October 2019) originating from the central Yangtze River and its tributary, the Hanshui River in Wuhan. The sum concentrations of FPs (ΣFPs) in the tap water in China ranged from not detected (ND) to 5.07 (median: 0.03 ng/L), with FP found in 55.3% of the samples, and other targets ≤ 50.0%. Significant regional variations in the ΣFPs values were found between East China (75th percentile: 0.31 ng/L) and Northwest China (0.04), as well as between East China and North China (0.04). Similar ΣFPs values were found for ground water and tap water. The estimated daily intake of ΣFPs via water ingestion was below 200 pg/kg-bw/day for all age groups and was lower than the reference dose for FP (0.2 μg/kg-bw/day). Additionally, FPs were found in all of the source water samples collected in Wuhan with concentrations in the range of 0.84–2.72 ng/L for ΣFPs (median: 2.39). Most of these FPs were removed during water treatment. Higher concentration of ΣFPs in tap water was observed in July (median: 0.04 ng/L) compared to that in October (ND). This is the first study on the occurrence of FPs in the Yangtze River, the fate of FPs during the tap water treatment, and the regional distribution of FPs in tap water from China. © 2020 Elsevier Ltd

语种： 英文

作者： Qi Mingxu;He Li;Ma Xiaofeng;Li Zili*

期刊： Bioscience, Biotechnology, and Biochemistry,2020年84(7):1353-1361 ISSN：0916-8451

通讯作者： Li Zili

作者机构： [Qi Mingxu; Ma Xiaofeng; Li Zili] Univ South China, Affiliated Nanhua Hosp, Dept Cardiovasc Med, Hengyang, Hunan, Peoples R China.;[He Li] Hengyang Second Peoples Hosp, Dept Psychiat, Hengyang, Hunan, Peoples R China.

通讯机构： [Li Zili] U;Univ South China, Affiliated Nanhua Hosp, Dept Cardiovasc Med, Hengyang, Hunan, Peoples R China.

关键词： Hypoxia-reoxygenation;SIRT1;apoptosis;cardiomyocytes;miR-181a-5p

摘要： MiR-181a-5p’s mechanism in hypoxia–reoxygenation (H/R)-induced cardiomyocytes apoptosis has not been clarified. This study verified that SIRT1 was the target of miR-181a-5p. MiR-181a-5p expression was up-regulated or down-regulated in H/R-induced cardiomyocytes, and SIRT1 was transfected into cells alone or in combination with miR-181a-5p. Cell viability, apoptosis, levels of released lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD), as well as the Bcl-2, Bax, and Caspase 3 levels in treated cells were tested. On the one hand, down-regulated miR-181a-5p promoted cell viability, reduced released LDH and MDA, and increased SOD level in H/R-induced cardiomyocytes. On the other hand, miR-181a-5p inhibited apoptosis and elevated Bcl-2 expression while decreasing the expressions of Bax and Caspase 3 in treated cells, but the effects of miR-181a-5p could be rescued by SIRT1. In conclusion, miR-181a-5p involved in H/R-induced cardiomyocytes apoptosis through regulating SIRT1, which might become a novel direction for related diseases. © 2020, © 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

语种： 英文

作者： Wang, Bo;Wang, Shuo;Zhang, Qi;Deng, Yixuan;Li, Xiang;...

期刊： Acta Biomaterialia,2019年96:55-67 ISSN：1742-7061

通讯作者： Kuang, Xin;Sheng, Shihou;Yu, Yingjie

作者机构： [Kuang, Xin; Wang, Bo; Deng, Yixuan; Peng, Liangyu; Li, Xiang] Univ South China, Affiliated Hosp 1, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.;[Wang, Shuo] PLA Rocket Force Characterist Med Ctr, Dept Pharm, Beijing 100088, Peoples R China.;[Zhang, Qi] NYU, Dept Chem & Biomol Engn, Brooklyn, NY 11201 USA.;[Zuo, Xianghao] SUNY Stony Brook, Dept Mat Sci & Chem Engn, Stony Brook, NY 11794 USA.;[Piao, Meihua] Jilin Univ, Hosp 1, Dept Anesthesiol, Changchun 130021, Jilin, Peoples R China.

通讯机构： [Kuang, Xin] U;[Sheng, Shihou] J;[Yu, Yingjie] S;Univ South China, Affiliated Hosp 1, Dept Anesthesiol, Hengyang 421001, Hunan, Peoples R China.;Jilin Univ, China Japan Union Hosp, Dept Gastrointestinal Surg, Changchun 130033, Jilin, Peoples R China.

关键词： Drug delivery system;Local anesthetics;Polymer

摘要： Local anesthetics, which cause temporary loss of pain by inhibiting the transmission of nerve impulses, have been widely used in clinical practice. However, neurotoxicity and short half-lives have significantly limited their clinical applications. To overcome those barriers, numerous drug delivery systems (DDS) have been designed to encapsulate local anesthetic agents, so that large doses can be released slowly and provide analgesia over a prolonged period. So far, multiple classes of local anesthetic carriers have been investigated, with some of them already on the market. Among those, polymer-based delivery platforms are the most extensively explored, especially in the form of polymeric nanoparticle carriers. This review gives a specific focus on the most commonly used natural and synthetic polymers for local anesthetics delivery, owing to their excellent biocompatibility, biodegradability and versatility. State-of-the-art studies concerning such polymer delivery systems have been discussed in depth. We also highlight the impact of those delivery platforms as well as some key challenges that need to be overcome for their broader clinical applications. Statement of significance: Currently, local anesthetics have been widely used in clinically practices to prevent transmission of nerve impulses. However, the applications of anesthetics are greatly limited due to their neurotoxicity and short half-lives. Moreover, it is difficult to maintain frequent administrations which can cause poor compliance and serious consequences. Numerous drug delivery systems have been developed to solve those issues. In this review, we highlight the recent advances in polymer-based drug delivery systems for local anesthetics. The advantages as well as shortcomings for different types of polymer-based drug delivery systems are summarized in this paper. In the end, we also give prospects for future development of polymer drug delivery systems for anesthetics. © 2019 Acta Materialia Inc.

语种： 英文

作者： Shi, Wenyuan;Zhao, Lanhua;Xu, Yujuan;Xu, Guizhen;Zeng, Yanhua*

期刊： Biotechnology & Biotechnological Equipment,2019年33(1):1034-1041 ISSN：1310-2818

通讯作者： Zeng, Yanhua

作者机构： [Shi, Wenyuan; Xu, Yujuan; Xu, Guizhen] Chenzhou First Peoples Hosp, Dept Clin Lab, Chenzhou, Peoples R China.;[Shi, Wenyuan; Xu, Yujuan; Xu, Guizhen] Univ South China, Inst Translat Med, Chenzhou, Peoples R China.;[Shi, Wenyuan; Xu, Yujuan; Xu, Guizhen] Southern Med Univ, Chenzhou Hosp, Chenzhou, Peoples R China.;[Zhao, Lanhua; Zeng, Yanhua] Univ South China, Hengyang Med Coll, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Peoples R China.

通讯机构： [Zeng, Yanhua] U;Univ South China, Hengyang Med Coll, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Peoples R China.

关键词： mimic epitope;Mycoplasma pneumoniae;P1 protein;serological diagnosis

摘要： Traditional diagnostic methods cannot meet the requirements for rapid diagnosis of Mycoplasma pneumoniae. Therefore, developing a serological diagnostic method with high specificity and sensitivity is urgent. In this study, we expressed and purified the recombinant P1’ protein, which comprises amino-acid residues 1160–1498 of the P1 protein. Thereafter, after immunising New Zealand rabbits, we purified the anti-P1’ polyclonal antibody using cyanogen bromide-activated sepharose 4B. By using this purified antibody as target molecule, we then performed a 4-round biopanning for a phage display random 12-mer peptide library. After sequencing analysis, we found that the polypeptide HLQMRLTKLRMP was a 12-mer peptide specifically binding to the anti-P1' polyclonal antibody and had 75% homology with the 1266-1272 amino acid (QVRTKLR) of the M. pneumoniae P1 protein. Besides, we further confirmed that the representative phage 1 which displayed this peptide could specifically bind to the anti-P1’ polyclonal antibody by dot immunobinding assay. Indirect ELISA showed that the synthesized 12-mer peptide could specifically bind to the serum of M. pneumoniae positive patients. The sensitivity and the specificity were 81.87% and 95%, respectively. These results indicated that HLQMRLTKLRMP might be a dominant epitope of the P1 and has the potential for serological diagnosis of M. pneumoniae. © 2019, © 2019 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science.

语种： 英文

作者： Zuo, Lingling;Sun, Hedong;Yu, Minjun;You, Xiaoxing;Zeng, Yanhua;...

期刊： Biotechnology & Biotechnological Equipment,2018年32(6):1590-1597 ISSN：1310-2818

通讯作者： Wu, Yimou

作者机构： [Zuo, Lingling] Univ South China, Affiliated Hosp 2, Dept Transfus Med, Hengyang, Peoples R China.;[Wu, Yimou; Zuo, Lingling; Yu, Minjun; You, Xiaoxing; Zeng, Yanhua] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Inst Pathogen Biol, Med Coll, Hengyang, Peoples R China.;[Wu, Yimou; Zuo, Lingling; Yu, Minjun; You, Xiaoxing; Zeng, Yanhua] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.;[Sun, Hedong] Univ South China, Dept Neurol, Affiliated Hosp 2, Hengyang, Peoples R China.

通讯机构： [Wu, Yimou] U;[Wu, Yimou] H;Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Inst Pathogen Biol, Med Coll, Hengyang, Peoples R China.;Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.

关键词： Mycoplasma genitalium lipoproteins;LAMPs;tumour necrosis factor alpha (TNF-alpha);apoptosis;HeLa cells

摘要： This aim of this study was to investigate the effects of Mycoplasma genitalium lipoproteins on the tumour necrosis factor α (TNF-α)-induced apoptosis in HeLa cells. We separated the M. genitalium lipid-associated membrane proteins (LAMPs) from M. genitalium and used them to treat HeLa cells. Apoptosis was induced by TNF-α treatment. Cell cycle and apoptosis were detected by flow cytometry. Pro-inflammatory cytokine contents were determined by enzyme-linked immunosorbent assay (ELISA). Mitochondrial membrane potential and caspase-3 protease activity were also measured. There was no significant difference in the viable cell percentage in HeLa cells treated with M. genitalium LAMPs at 0–20 μg/mL. However, when treated with 40 μg/mL LAMPs, cytocidal activity was observed during the first 24 h. M. genitalium LAMPs induced cell cycle arrest at the G 1 phase in HeLa cells. When apoptosis was induced by TNF-α in HeLa cells, M. genitalium LAMPs significantly reduced the percentage of apoptotic cells. In addition, M. genitalium LAMPs significantly reduced the loss of mitochondrial membrane potential and caspase-3 protease activity in TNF-α-treated HeLa cells. However, the blocking of NF-κB significantly increased the mitochondrial membrane potential and caspase-3 protease activity in TNF-α-induced HeLa cells treated with M. genitalium LAMPs. The obtained results suggest that M. genitalium LAMPs could inhibit TNF-α-induced apoptosis in HeLa cells, which would contribute to the understanding of the pathogenesis of M. genitalium. © 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文