摘要：
Predictive estimation of radioactive sources using Bayesian theory is of great importance in nuclear emergency response. To this aim, we put forward an unscented particle filtering algorithm based on divide-and-conquer sampling. Our method exploits the information acquired by mobile detection robots to search for radioactive sources at unknown locations. Firstly, we assume a circular and extremely thin NaI (TI) scintillator detector, then analyze the geometric relationship between the detector cross-sectional area and the square of the radiation source distance, and finally establish a radiation source search model in accordance with Bayesian method. We also introduce a divide-and-conquer strategy to solve the problems of particle dilution and particle degradation, so as to achieve higher search accuracy and speed. Through simulation experiments, compared with standard particle filter and unscented particle filter, the algorithm can effectively improve particle diversity and successfully search radioactive sources in a shorter time.
作者机构：
[Luo, Haiyan; Wang, Ting; Tang, Wenying; Tang, Ying; Liao, Hongwu] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;[Wang, Ting; Tang, Wenying; Tang, Ying; Liao, Hongwu] Univ South China, Hengyang Med Sch, Sch Nursing, Hengyang 421002, Hunan, Peoples R China.;[Wang, Ting; Tang, Wenying; Tang, Ying; Liao, Hongwu] Univ South China, Affiliated Nanhua Hosp, Hlth Sch Nucl Ind, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;[Chen, Qiuyu; Tang, Qiaojin] Univ South China, Affiliated Nanhua Hosp, Dept Gynecol & Matern, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;[Zhang, Xuehui] Hengyang Maternal & Child Hlth Care Hosp, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Hongwu] U;[Yang, Lihua] X;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Sch Nursing, Hengyang 421002, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hlth Sch Nucl Ind, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.
摘要：
Prenatal diagnosis is an important means of early diagnosis of genetic diseases, which can effectively reduce the risk of birth defects. Free fetal cells, as a carrier of intact fetal genetic material, provide hope for the development of high-sensitivity and high-accuracy prenatal diagnosis technology. However, the number of fetal cells is small and it is difficult to apply clinically. In recent years, noninvasive prenatal diagnosis (NIPD) technology for fetal genetic material in maternal peripheral blood has developed rapidly, which makes it possible to diagnose genetic diseases by fetal cells in maternal peripheral blood. This article reviewed the current status of fetal cell separation and enrichment technology and its application in noninvasive prenatal diagnosis technology.
作者：
Hamza, Mohammed F.;Wei, Yuezhou;Althumayri, Khalid;Fouda, Amr;Hamad, Nora A.
期刊：
Materials,2022年15(13):4676- ISSN：1996-1944
通讯作者：
Mohammed F. Hamza<&wdkj&>Yuezhou Wei
作者机构：
[Wei, Yuezhou; Hamza, Mohammed F.] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Hamza, Mohammed F.] Nucl Mat Author, POB 530, Cairo 11728, Egypt.;[Wei, Yuezhou] Shanghai Jiao Tong Univ, Sch Nucl Sci & Engn, Shanghai 200240, Peoples R China.;[Althumayri, Khalid] Taibah Univ, Coll Sci, Dept Chem, Al Madinah Al Munawarah 30002, Saudi Arabia.;[Fouda, Amr] Al Azhar Univ, Fac Sci, Bot & Microbiol Dept, Cairo 11884, Egypt.
通讯机构：
[Mohammed F. Hamza; Yuezhou Wei] A;Authors to whom correspondence should be addressed.<&wdkj&>Nuclear Materials Authority, P.O. Box 530, El-Maadi, Cairo 11728, Egypt<&wdkj&>School of Nuclear Science and Technology, University of South China, Hengyang 421001, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>School of Nuclear Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China<&wdkj&>School of Nuclear Science and Technology, University of South China, Hengyang 421001, China
期刊：
Science of The Total Environment,2022年802:149801 ISSN：0048-9697
通讯作者：
Ki, Jang-Seu
作者机构：
[Wang, Hui; Ki, Jang-Seu; Park, Hyunjun; Kim, Hansol] Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;[Wang, Hui] Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构：
[Ki, Jang-Seu] S;Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.
摘要：
Temperature may affect the production of saxitoxin (STX) and its derivatives (STXs); however, this is still contro-versial. Further, STX-biosynthesis gene regulation and the relation of its toxicity with temperature are not clearly understood. In the present study, we evaluated the effects of different temperatures (12 degrees C, 16 degrees C, and 20 degrees C) on the growth, toxin profiles, and expression of two core STX-biosynthesis genes, sxtA and sxtG, in the toxic dinofla-gellate Alexandrium pacificum Alex05, isolated from Korean coasts. We found that temperature significantly af-fected cell growth, with maximum growth recorded at 16 degrees C, followed by 20 degrees C and 12 degrees C. HPLC analysis revealed mostly 12 of STXs from the tested cultures. Interestingly, the contents of STXs increased in the cells cul-tured at 16 degrees C and exposed to cold stress, compared to the 20 degrees C culture and heat stress; however, toxin compo-nents were much more diverse under heat stress. These toxin profiles generally matched with the sxtA and sxtG expression levels. Incubation at lower temperatures (12 degrees C and 16 degrees C) and exposure to cold stress increased sxtA and sxtG expressions in the cells, whereas heat stress showed little change or downregulated the transcription of both genes. Principal component analysis (PCA) showed low correlation between STXs eq and expressional levels of sxtA and sxtG in heat-stressed cells. These results suggest that temperature might be a crucial factor affecting the level and biosynthesis of STXs in marine toxic dinoflagellates. (c) 2021 Elsevier B.V. All rights reserved.
作者机构：
[Shi, Qiang; Xu, Yuxia] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Spine Surg, Changsha 410018, Peoples R China.;[Chen, Shu; Yan, Hua] Shantou Univ, Dept Pediat Orthoped, Guangzhou Huaxin Orthoped Hosp, Guangzhou 510507, Peoples R China.;[Cao, Qian] Xiangtan Chinese Med Hosp, Dept Orthoped, Xiangtan 411100, Peoples R China.
通讯机构：
[Qian Cao; Yuxia Xu] D;Department of Orthopedics, Xiangtan Chinese Medicine Hospital, Xiangtan, People’s Republic of China<&wdkj&>Department of Spine Surgery, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, People’s Republic of China
摘要：
Background This study aimed to assess the clinical and radiologic outcomes of a functional brace in combination with physical therapy (FBPT) for early correction of cubitus varus in young children. Methods Eighteen consecutive patients with cubitus varus secondary to supracondylar fractures were enrolled between July 2017 and March 2019. We used the FBPT technique to correct varus and sagittal plane deformity for early cubitus varus in young children. The clinical evaluation included measurement of varus angulation, sagittal plane, and range of motion at three, six, and twelve months post-intervention. The clinical and radiographic results were assessed according to the Bellemore criteria. Results Pre-treatment humerus-elbow-wrist (HEW) angle measured on the affected side (varus deformity) ranged between -38 degrees and -12 degrees (average, -23.2 degrees) while the post-treatment HEW angle ranged between -10 degrees and + 15 degrees (average, 8.8 degrees). Compared with the unaffected side, no statistically significant difference was found in the affected side post-intervention (P > 0.05). According to the Bellemore criteria, we got excellent results in fourteen patients (77.8%), good results in three patients (16.7%), and poor result in one patient (5.5%). All patients and their parents (except one patient with residual varus deformities) were satisfied with the functional and cosmetic outcomes. Conclusions The FBPT is effective for the treatment of cubitus varus in children, especially for young children within 6 months of the injury.
通讯机构：
[Le Li] C;College of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, People’s Republic of China<&wdkj&>Hengyang Key Laboratory for Comprehensive Prevention and Control of Uranium Contamination and its Health Hazards, University of South China, Hengyang 421001, Hunan, People’s Republic of China<&wdkj&>Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, University of South China, Hengyang 421001, People’s Republic of China
关键词：
Uranyl ion;DNAzyme;Amplification;Sensor
摘要：
An ultra-sensitive method for detection of UO22+ was developed based on MoS2 nanosheet and the principle of entropy-driven amplification. UO22+-specific DNAzyme was cleaved to release DNA fragments. The cyclic amplification reaction was initiated by the DNA fragment, and DNA with the fluorophore in the DNA complex is released. MoS2 has a high affinity for ssDNA, but a low affinity for dsDNA. The ssDNA with fluorophore binds to MoS2 based on this characteristic, and the fluorescence intensity is greatly reduced. The degree of fluorescence intensity change has a linear relationship with UO22+ in the range of 10-100 pM, and the detection limit is 3.6 pM. This method has been successfully applied to the detection of UO22+ in water samples and has shown certain potential in the prevention and control of environmental pollution.
摘要：
N6-methyladenosine (m6A) methylation, the most common form of internal RNA modification in eukaryotes, has gained increasing attention and become a hot research topic in recent years. M6A plays multifunctional roles in normal and abnormal biological processes, and its role may vary greatly depending on the position of the m6A motif. Programmed cell death (PCD) includes apoptosis, autophagy, pyroptosis, necroptosis and ferroptosis, most of which involve the breakdown of the plasma membrane. Based on the implications of m6A methylation on PCD, the regulators and functional roles of m6A methylation were comprehensively studied and reported. In this review, we focus on the high-complexity links between m6A and different types of PCD pathways, which are then closely associated with the initiation, progression and resistance of cancer. Herein, clarifying the relationship between m6A and PCD is of great significance to provide novel strategies for cancer treatment, and has a great potential prospect of clinical application.
作者机构：
[Chu, Hanyu; Yang, Fei] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Chen, Jihua; Du, Can; Feng, Xiangling; Yang, Fei; Yang, Yue] Cent South Univ, Xiangya Sch Publ Hlth, Changsha 410078, Peoples R China.;[Zhu, Lemei] Changsha Med Univ, Sch Publ Hlth, Changsha 410219, Peoples R China.;[Yang, Fei] Univ South China, Sch Basic Med Sci, Key Lab Ecol Environm & Crit Human Dis Prevent Hun, Dept Educ,Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Jihua Chen; Fei Yang] A;Authors to whom correspondence should be addressed.<&wdkj&>Xiangya School of Public Health, Central South University, Changsha 410078, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>The Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province, Department of Education, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Xiangya School of Public Health, Central South University, Changsha 410078, China<&wdkj&>Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, China
摘要：
Obesity, a metabolic disease caused by excessive fat accumulation in the body, has attracted worldwide attention. Microcystin-LR (MC-LR) is a hepatotoxic cyanotoxin which has been reportedly to cause lipid metabolism disorder. In this study, C57BL/6J mice were fed a high-fat diet (HFD) for eight weeks to build obese an animal model, and subsequently, the obese mice were fed MC-LR for another eight weeks, and we aimed to determine how MC-LR exposure affects the liver lipid metabolism in high-fat-diet-induced obese mice. The results show that MC-LR increased the obese mice serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), indicating damaged liver function. The lipid parameters include serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), and liver TG, which were all increased, whilst the high-density lipoprotein cholesterol (HDL-c) was decreased. Furthermore, after MC-LR treatment, histopathological observation revealed that the number of red lipid droplets increased, and that steatosis was more severe in the obese mice. In addition, the lipid synthesis-related genes were increased and the fatty acid beta-oxidation-related genes were decreased in the obese mice after MC-LR exposure. Meanwhile, the protein expression levels of phosphorylation phosphatidylinositol 3-kinase (p-PI3K), phosphorylation protein kinase B (p-AKT), phosphorylation mammalian target of rapamycin (p-mTOR), and sterol regulatory element binding protein 1c (SREBP1-c) were increased; similarly, the p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR, and SREBP1/beta-actin were significantly up-regulated in obese mice after being exposed to MC-LR, and the activated PI3K/AKT/mTOR/SREBP1 signaling pathway. In addition, MC-LR exposure reduced the activity of superoxide dismutase (SOD) and increased the level of malondialdehyde (MDA) in the obese mice's serum. In summary, the MC-LR could aggravate the HFD-induced obese mice liver lipid metabolism disorder by activating the PI3K/AKT/mTOR/SREBP1 signaling pathway to hepatocytes, increasing the SREBP1-c-regulated key enzymes for lipid synthesis, and blocking fatty acid beta-oxidation.
摘要：
Apolipoprotein A-I binding protein (AIBP), a secreted protein, has been shown to play a pivotal role in the development of atherosclerosis. The function of intracellular AIBP, however, is not yet well characterized. Here, we found that AIBP is abundantly expressed within human and mouse atherosclerotic lesions and exhibits a distinct localization in the inner membrane of mitochondria in macrophages. Bone marrow-specific AIBP deficiency promotes the progression of atherosclerosis and increases macrophage infiltration and inflammation in low-density lipoprotein receptor-deficient (LDLR−/−) mice. Specifically, the lack of mitochondrial AIBP leads to mitochondrial metabolic disorders, thereby reducing the formation of mitophagy by promoting the cleavage of PTEN-induced putative kinase 1 (PINK1). With the reduction in mitochondrial autophagy, macrophages polarize to the M1 proinflammatory phenotype, which further promotes the development of atherosclerosis. Based on these results, mitochondrial AIBP in macrophages performs an antiatherosclerotic role by regulating of PINK1-dependent mitophagy and M1/M2 polarization.
期刊：
PAKISTAN JOURNAL OF MEDICAL SCIENCES,2022年38(5) ISSN：1682-024X
通讯作者：
Liu, CQ
作者机构：
[Chen, Feng] Univ South China, Hengyang Med Sch, Affiliated Nanhua Hosp, Dept Anorectal, Hengyang 421002, Hunan, Peoples R China.;[Liu, Changqing] Univ South China, Hengyang Med Sch, Affiliated Nanhua Hosp, Dept Operating Room, 336 Dongfeng South Rd, Hengyang 421002, Hunan, Peoples R China.
通讯机构：
[Liu, CQ ] U;Univ South China, Hengyang Med Sch, Affiliated Nanhua Hosp, Dept Operating Room, 336 Dongfeng South Rd, Hengyang 421002, Hunan, Peoples R China.
关键词：
Necrotizing fasciitis;Silver ion sterilization nursing solution;Negative pressure sealing drainage technology;Wound healing time
摘要：
Objectives: To study the application effect of negative pressure sealing drainage technology combined with silver ion sterilization nursing solution in the nursing of necrotizing fasciitis. Methods: Medical records of patients with necrotizing fasciitis, treated in our hospital from June 2019 to June 2021, were selected. Patients were retrospectively assigned into two groups based on the debridement method used: debridement with silver ion sterilization nursing solution Group-I, or debridement with negative pressure sealing drainage technology combined with silver ion sterilization nursing solution. Group-II. Wound healing, dressing change times, healing time, treatment cost and patient satisfaction in both groups were statistically compared. Results: The wound healing rate of patients in Group-II group was higher than that of Group-I on the 5th, 10th and 15th day after operation (P < 0.05). Dressing change times, healing time and treatment cost of patients in the Group-II were lower than those of Group-1 (P < 0.05). Patient satisfaction in the Group-II was 91.4% (54 / 59), which was higher than that of Group-I (75.4% (40 / 53) (P < 0.05). Conclusions: Negative pressure sealing drainage technology combined with silver ion sterilization nursing solution in the nursing of necrotizing fasciitis is effective. It can promote wound healing, shorten the healing time, reduce the times of wound dressing change and treatment cost. It also improves the overall patient satisfaction, making it an efficient method in clinical application.
作者机构：
[Luo, Min; Cai, Yongqin; Tang, Lixin; Lei, Xin; Wang, Jinyuan; Liu, Jiang; Lei, Xiaocan; Ma, Duo; Zhao, Leilei; Long, Shuanglian] Univ South China, Clin Anat & Reprod Med Applicat Inst, Hengyang Med Sch, Dept Histol & Embryol, Hengyang 421001, Peoples R China.;[Wang, Junli; Liao, Biyun] Youjiang Med Univ Nationalities, Reprod Med Ctr, Affiliated Hosp, Baise 533000, Peoples R China.;[Yang, Fenglian] Youjiang Med Univ Nationalities, Key Lab Environm Pollut & Hlth Risk Assessment, Baise 533000, Peoples R China.
通讯机构：
[Long, Shuanglian; Lei, Xiaocan] C;[Yang, Fenglian] K;Clinical Anatomy and Reproductive Medicine Application Institute, Department of Histology and Embryology, Hengyang Medical School, University of South China, Hengyang 421001, China. Electronic address:;Key Laboratory of Environmental Pollution and Health Risk Assessment, Youjiang Medical University for Nationalities, Baise 533000, China. Electronic address:
摘要：
Dysfunction of spermatogenesis is a major complication of diabetes mellitus (DM). This study characterized the protective effects of Dendrobium nobile-derived polysaccharides (DNP) against spermatogenetic dysfunction in mice with streptozotocin (STZ)-induced diabetes. The diabetic mice had lower body and testicular mass, and fewer spermatozoa with a higher incidence of malformation. The testicular histology showed disordered narrow seminiferous tubules covering a smaller area, and fewer spermatogenic cells. Moreover, the qRT-PCR analysis indicated that DM was associated with high expression of the pro-apoptotic factor Bax and low expression of the anti-apoptotic factor Bcl-2 in the testes. The qRT-PCR and immunohistochemical analysis clarified that DM was also associated with low testicular expression of the Sertoli cell (SC) markers GATA-4, WT1, and vimentin, and genes encoding the glycolytic rate-limiting enzymes LDHA, PKM2, and HK2. DNP treatment increased the body and testicular masses, sperm count, and number of spermatogenic cells of the mice, and reduced the proportion of abnormal sperm. DNP also reduced the expression of Bax, and increased that of Bcl-2, GATA-4, WT1, vimentin, LDHA, PKM2, and HK2, in the testes of the diabetic mice. Thus, DNP protects against spermatogenic dysfunction in diabetic mice by inhibiting apoptosis and activating the glycolytic pathway in their testes.
作者机构：
[Liu, Bing] Univ South China, Hengyang 421001, Peoples R China.;[Wang, Hongqing; Cui, Wenbin] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Zhou, Jianliang] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Hongqing Wang] S;School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Developing a fluorescent probe for UO22+, which is resistant to interference from other ions such as Cu2+ and can be applied in acidic and high-water systems, has been a major challenge. In this study, a "turn-off" fluorescent probe for triamine-modified flavonoid derivatives, 2-triphenylamine3-hydroxy-4H-chromen-4-one (abbreviated to HTPAF), was synthesized. In the solvent system of dimethyl sulfoxide:H2O (abbreviated to DMSO:H2O) (v/v = 5:95 pH = 4.5), the HTPAF solution was excited with 364 nm light and showed a strong fluorescence emission peak at 474 nm with a Stokes shift of 110 nm. After the addition of UO22+, the fluorescence at 474 nm was quenched. More importantly, there was no interference in the presence of metal ions (Pb2+, Cd2+, Cr3+, Fe3+, Co2+, Th4+, La3+, etc.), especially Cu2+ and Al3+. It is worth noting that the theoretical model for the binding of UO22+ to HTPAF was derived by more detailed density functional theory (DFT) calculations in this study, while the coordination mode was further verified using HRMS, FT-IR and (HNMR)-H-1, demonstrating a coordination ratio of 1:2. In addition, the corresponding photo-induced electron transfer (PET) fluorescence quenching mechanism was also proposed.
摘要：
Because the inelastic impact of high flux, high-energy neutrons on tungsten material in a commercial fusion reactor would drastically decrease the service life and steady-state functioning of the core plasma in the future, it is vital to investigate the damage process. In this paper, a particle sputtering theory ap-proach is used to derive an equation for calculating the sputtering yield of high-energy neutrons by sim-ulating the transport of primary knocked-out atoms (PKA) resulting from inelastic collisions of 14.1 MeV neutrons with tungsten materials at different depth levels, namely Y = integral Emax E 0 f(E) *Y(E)dE , where f (E) is the energy probability distribution function of PKA, Y (E) is the sputtering yield at a given energy of PKA particles generated by inelastic scattering collisions, Emax is the maximum kinetic energy of PKA, and E 0 is the minimum kinetic energy of PKA (generally taken as 0). According to the result, when the neutron wall load is 1 MW/m2, and the annual average equivalent material wastage is less than 1 nm, sputtering damage to tungsten material can be ignored. However, it must be kept in mind that the reflection of the PKA into the plasma will have an impact on the performance of the plasma confinement system and the damage caused by the PKA's internal energy. (c) 2022 Elsevier B.V. All rights reserved.
