摘要：
Heap leaching uranium tailings (HLUTs) contain radionuclides and sulfuric acid, presenting some challenges for the disposal of cemented HLUTs backfill (CUTB), including low stability and potential environmental contamination. In light of these issues, using a blend of cement clinker (CL) and high content phosphorus slag (PS) as binders to replace traditional cement for the preparation of green and cost-effective CUTB were proposed. The fluidity, setting time (ST), mechanical properties, Radon (Rn) exhalation rate, uranium (U) occurrence form, U (VI) leaching behavior, and microstructure of CUTB with different PS contents were investigated. The results showed that the fluidity, bleeding rate and ST of slurry (PS content >40%) met the filling requirements. The strength of PS-based CUTB was initially low, but rapidly increased in subsequent periods, surpassing 2 MPa at 28 d and 4.5 MPa at 90 d (with 50-70% PS content). Moreover, the Rn exhalation rates and U (VI) leaching levels of CUTB were far lower than the stipulated limits. When PS content exceeded 50%, the migratory uranium content in CUTB was below 10% with low migration risks. With the addition of PS, the amount and degree of polymerization of C-S-H gels in the matrix increased, while gypsum and CH content decreased. The S/S of U (VI) and SO42- was attributed to the combined effects of chemical immobilization of nascent minerals and physical adsorption/encapsulation of hydration products. Additionally, these hydrates effectively sealed micro-cracks on HLUTs particles surfaces and filled interlocking pores to decrease Rn exhalation. PS-based CUTB exhibited a high capacity increasing ratio and low cost. This research can promote the utilization of hazardous solid wastes such as HLUTs, reduce carbon dioxide emissions, and provide a theoretical basis for ensuring green and safe production in mining.
摘要：
Canopy FGF signaling regulator 2 (CNPY2) is a novel angiogenic growth factor. In recent years, increasing evidence highlights that CNPY2 has important functions in health and disease. Many new blood vessels need to be formed to meet the nutrient supply in the process of tumor growth. CNPY2 can participate in the development of tumors by promoting angiogenesis. CNPY2 also enhances neurite outgrowth in neurologic diseases and promotes cell proliferation and tissue repair, thereby improving cardiac function in cardiovascular diseases. Regrettably, there are few studies on CNPY2 in various diseases. At the same time, its biological function and molecular mechanism in the process and development of disease are still unclear. This paper reviews the recent studies on CNPY2 in cervical cancer, renal cell carcinoma, prostate cancer, colorectal cancer, lung cancer, gastric cancer, hepatocellular carcinoma, cerebral ischemia-reperfusion injury, spinal cord ischemia-reperfusion injury, Parkinson's disease, ischemic heart disease, myocardial ischemia-reperfusion injury, myocardial infarction, heart failure, and non-alcoholic fatty liver disease. The biological function and molecular mechanism of CNPY2 in these diseases have been summarized in this paper. Many drugs that play protective roles in tumors, cardiovascular diseases, non-alcoholic fatty liver disease, and neurologic diseases by targeting CNPY2, have also been summarized in this paper. In addition, the paper also details the biological functions and roles of canopy FGF signaling regulator 1 (CNPY1), canopy FGF signaling regulator 3 (CNPY3), canopy FGF signaling regulator 4 (CNPY4), and canopy FGF signaling regulator 5 (CNPY5). The mechanism and function of CNPY2 should be continued to study in order to accelerate disease prevention in the future.
作者机构：
[Li, Ye; Zhang, Yuexin; Li, Chunquan; Zhao, Yu; Huang, Xuemei; Xie, Liyuan; Zhang, Guorui; Zhao, Jun; Fan, Shifan; Song, Chao] Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Li, Ye; Zhang, Yuexin; Li, Chunquan; Zhao, Yu; Huang, Xuemei; Xie, Liyuan; Zhang, Guorui; Zhao, Jun; Fan, Shifan; Song, Chao] Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.;[Li, Ye; Zhang, Yuexin; Zhang, Qinyi; Li, Chunquan; Zhao, Yu; Huang, Xuemei; Xie, Liyuan; Zhang, Guorui; Fan, Shifan; Song, Chao] Univ South China, Sch Comp, Hengyang 421001, Hunan, Peoples R China.;[Li, Chunquan] Univ South China, Hunan Prov Maternal & Child Hlth Care Hosp, Hengyang Med Sch, Key Lab Birth Defect Res & Prevent,Natl Hlth Commi, Hengyang 421001, Hunan, Peoples R China.;[Li, Ye; Zhang, Yuexin; Li, Chunquan; Zhao, Yu; Huang, Xuemei; Xie, Liyuan; Zhang, Guorui; Fan, Shifan; Song, Chao] Univ South China, Affiliated Hosp 1, Cardiovasc Lab Big Data & Imaging ArtificialIntell, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, CQ ] U;Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Comp, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Prov Maternal & Child Hlth Care Hosp, Hengyang Med Sch, Key Lab Birth Defect Res & Prevent,Natl Hlth Commi, Hengyang 421001, Hunan, Peoples R China.
关键词：
genes;mice;candidate disease gene;inference;multiomics;oncogenes;enhancer of transcription;cell lines;transcription factor
摘要：
Gene regulatory networks (GRNs) are interpretable graph models encompassing the regulatory interactions between transcription factors (TFs) and their downstream target genes. Making sense of the topology and dynamics of GRNs is fundamental to interpreting the mechanisms of disease etiology and translating corresponding findings into novel therapies. Recent advances in single-cell multi-omics techniques have prompted the computational inference of GRNs from single-cell transcriptomic and epigenomic data at an unprecedented resolution. Here, we present scGRN (https://bio.liclab.net/scGRN/), a comprehensive single-cell multi-omics gene regulatory network platform of human and mouse. The current version of scGRN catalogs 237 051 cell type-specific GRNs (62 999 692 TF-target gene pairs), covering 160 tissues/cell lines and 1324 single-cell samples. scGRN is the first resource documenting large-scale cell type-specific GRN information of diverse human and mouse conditions inferred from single-cell multi-omics data. We have implemented multiple online tools for effective GRN analysis, including differential TF-target network analysis, TF enrichment analysis, and pathway downstream analysis. We also provided details about TF binding to promoters, super-enhancers and typical enhancers of target genes in GRNs. Taken together, scGRN is an integrative and useful platform for searching, browsing, analyzing, visualizing and downloading GRNs of interest, enabling insight into the differences in regulatory mechanisms across diverse conditions. Graphical Abstract
期刊：
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES,2024年34(3):691-698 ISSN：0939-4753
通讯作者：
Ling Liu
作者机构：
[Zhang, Miao; Liu, Ling; Xie, Yingying; Tan, Yangrong; Xu, Jin; Chen, Hao; Qu, Peiliu; Zhu, Liyuan] Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, PR China;[Xie, Yingying] Department of Cardiology, China-Japan Friendship Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China;[Chen, Hao] Department of Cardiology, Second Affiliated Hospital of South China University, Hengyang, Hunan, PR China;[Zhang, Miao; Tan, Yangrong; Xu, Jin; Qu, Peiliu; Zhu, Liyuan] Modern Cardiovascular Disease Clinical Technology Research Center of Hunan Province, PR China;[Liu, Ling] Modern Cardiovascular Disease Clinical Technology Research Center of Hunan Province, PR China. Electronic address: feliuling@csu.edu.cn
通讯机构：
[Ling Liu] D;Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, PR China<&wdkj&>Modern Cardiovascular Disease Clinical Technology Research Center of Hunan Province, PR China
摘要：
BACKGROUND AND AIM: Evidence from prospective cohort studies has revealed an inverse association between cheese consumption and the development of atherosclerosis (AS), atherosclerotic cardiovascular diseases (ASCVD), and their complications. However, it remains unclear whether this observed association is influenced by potential confounding factors that may arise during the long-term development process of AS, ASCVD, and its complications. Therefore, to further clarify the causal relationship between cheese consumption and AS, ASCVD, and its complications, we conducted a two-sample Mendelian randomization (MR) analysis to explore the causal association between cheese intake and the aforementioned health outcomes. METHODS AND RESULTS: We employed a two-sample MR analysis based on publicly available genome-wide association studies (GWAS) to infer the causal relationship, with no overlap between their participating populations. The effect estimates were calculated using the random-effects inverse-variance-weighted method. Sensitivity analyses were conducted using Cochran's Q statistic, funnel plot, leave-one-out analysis, and MR-Egger intercept tests. The genetically predicted cheese intake was found to be associated with lower risks of coronary AS (odds ratio [OR]=0.72, 95% confidence interval [CI] 0.59-0.88, P=0.001), peripheral vascular AS (OR=0.56, 95% CI 0.37-0.84, P=0.006), other vascular AS (OR=0.66, 95% CI 0.44-0.99, P=0.043), coronary artery disease (OR=0.64, 95% CI 0.56-0.74, P=1.57e-09), angina pectoris (OR=0.70, 95% CI 0.58-0.84, P=4.92e-05), myocardial infarction (OR=0.63, 95% CI 0.52-0.77, P=3.56e-06), heart failure (OR=0.62, 0.49-0.79, P=1.20e-04), total ischemic stroke (OR=0.76, 95% CI 0.63-0.91, P=0.003), peripheral artery disease (OR=0.64, 95% CI 0.43-0.95, P=0.028), and cognitive impairment (OR=0.65, 95% CI 0.56-0.74, P=3.40e-10). However, no associations were observed for cerebrovascular AS, arrhythmia, cardiac death, ischemic stroke (large artery AS), ischemic stroke (small vessel), ischemic stroke (cardioembolic), and transient ischemic attack. CONCLUSION: This two-sample MR analysis reveals a causally inverse association between cheese intake and multi-vascular AS (including coronary AS, peripheral vascular AS, and other vascular AS), as well as multiple types of ASCVD and its complications (such as coronary artery disease, angina pectoris, myocardial infarction, heart failure, total ischemic stroke, and peripheral artery disease). The findings from this study may lay a stronger theoretical foundation and present new opportunities for the dietary management of future atherosclerotic cardiovascular diseases.
期刊：
FRONTIERS IN NEUROLOGY,2024年15:1322228 ISSN：1664-2295
通讯作者：
Zhu, Shuzhen;Wang, Q
作者机构：
[Zhu, Shuzhen; Li, Fangyi; Zhang, Wenjie; Deng, Bin; Zhu, SZ; Wang, Qing; Zhou, Hang; Wang, Q; Weng, Guomei; Luo, Yuqi] Southern Med Univ, Dept Neurol, Zhujiang Hosp, Guangzhou, Peoples R China.;[Li, Fangyi] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Neurol, Changsha, Peoples R China.;[Weng, Guomei] First Peoples Hosp Zhaoqing, Dept Neurol, Zhaoqing, Peoples R China.;[Tao, Xi] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Neurol Rehabil, Affiliated Hosp 1, Changsha, Peoples R China.;[Deng, Mingzhu] Brain Hosp Hunan Prov, Peoples Hosp Hunan Prov 2, Dept Neurol, Changsha, Peoples R China.
通讯机构：
[Wang, Q ; Zhu, SZ] S;Southern Med Univ, Dept Neurol, Zhujiang Hosp, Guangzhou, Peoples R China.
关键词：
Parkinson’s disease;inflammation;lymphocyte-to-monocyte ratio;neutrophil-to-high-density lipoprotein ratio;neutrophil-to-lymphocyte ratio
摘要：
BACKGROUND: Inflammation plays a pivotal role in the pathogenesis of Parkinson's disease (PD). However, the correlation between peripheral inflammatory markers and the severity of PD remains unclear. METHODS: The following items in plasma were collected for assessment among patients with PD (n = 303) and healthy controls (HCs; n = 303) were assessed for the neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and neutrophil-to-high-density-lipoprotein ratio (NHR) in plasma, and neuropsychological assessments were performed for all patients with PD. Spearman rank or Pearson correlation was used to evaluate the correlation between the NLR, the LMR and the NHR and the severity of PD. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance of the NLR, LMR and NHR for PD. RESULTS: The plasma NLR and NHR were substantially higher in patients with PD than in HCs, while the plasma LMR was substantially lower. The plasma NLR was positively correlated with Hoehn and Yahr staging scale (H&Y), Unified Parkinson's Disease Rating Scale (UPDRS), UPDRS-I, UPDRS-II, and UPDRS-III scores. Conversely, it exhibited a negative relationship with Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) scores. Furthermore, the plasma NHR was positively correlated with H&Y, UPDRS, UPDRS-I, UPDRS-II and UPDRS-III scores. Moreover, negative associations were established between the plasma LMR and H&Y, UPDRS, UPDRS-I, UPDRS-II, and UPDRS-III scores. Finally, based on the ROC curve analysis, the NLR, LMR and NHR exhibited respectable PD discriminating power. CONCLUSION: Our research indicates that a higher NLR and NHR and a lower LMR may be relevant for assessing the severity of PD and appear to be promising disease-state biomarker candidates.
通讯机构：
[Yin, XH ] U;Univ South China, Sch Nursing, Hengyang, Hunan, Peoples R China.
关键词：
Prehabilitation;Frailty;Aged;Meta-analysis
摘要：
Objective: The study investigates the impact of preoperative rehabilitation on the surgical prognosis of frail older patients. Method: The effect sizes of all studies retrieved and included by the nine databases were analyzed and expressed as RR and WMD. Results: 8 studies with 902 participants met the criteria for inclusion. A significant reduction in total complications (RR = 0.84, 95 % CI = 0.73 to 0.97, P = 0.021) and the 6MWT after surgery (WMD = 74.76, 95 % CI = 44.75 to 104.77, P = 0.000) was observed in the prehabilitation group. But it had no differences in mortality(RR = 1.89, 95 % CI = 0.75 to 4.72, P = 0.176), readmission rates(RR = 1.04, 95 % CI = 0.56 to 1.91, P = 0.906) and LOS(WMD = -0.24, 95 % CI = -1.00 to 0.52, P = 0.540). Conclusions: Prehabilitation had positive effect on postoperative complications and functional recovery in frail older patients. (c) 2023 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
摘要：
A novel reactive oxygen species‐responsive biodegradable nanoparticle (NP@PolyRHAPM) is designed, in which astaxanthin can reduce intracellular ROS levels and rapamycin‐enhanced autophagy levels, and restore mitochondrial function, hence successfully repolarizing M1 macrophages into M2 phenotypes. This repolarization enhances the proliferation and viability of chondrocytes while inhibiting apoptosis. Overall, this ROS‐responsive nanocarrier system provides a new avenue for progressive OA therapy. Abstract Osteoarthritis (OA) is a chronic joint disease characterized by synovitis and joint cartilage destruction. The severity of OA is highly associated with the imbalance between M1 and M2 synovial macrophages. In this study, a novel strategy is designed to modulate macrophage polarization by reducing intracellular reactive oxygen species (ROS) levels and regulating mitochondrial function. A ROS‐responsive polymer is synthesized to self‐assemble with astaxanthin and autophagy activator rapamycin to form nanoparticles (NP@PolyRHAPM). In vitro experiments show that NP@PolyRHAPM significantly reduced intracellular ROS levels. Furthermore, NP@PolyRHAPM restored mitochondrial membrane potential, increased glutathione (GSH) levels, and promoted intracellular autophagy, hence successfully repolarizing M1 macrophages into the M2 phenotype. This repolarization enhanced chondrocyte proliferation and vitality while inhibiting apoptosis. In vivo experiments utilizing an anterior cruciate ligament transection (ACLT)‐induced OA mouse model revealed the anti‐inflammatory and cartilage‐protective effects of NP@PolyRHAPM, effectively mitigating OA progression. Consequently, the findings suggest that intra‐articular delivery of ROS‐responsive nanocarrier systems holds significant promise as a potential and effective therapeutic strategy for OA treatment.
期刊：
Materials Science and Engineering B-Advanced Functional Solid-State Materials,2024年302:117245 ISSN：0921-5107
通讯作者：
Gao, F
作者机构：
[Fujita, Toyohisa; Wang, Xinpeng; Gao, F; Li, Ao; Huang, Jiaming; Mi, Shuxin; Gao, Feng; Ren, Feng] Guangxi Univ, State Key Lab Featured Met Mat & Life Cycle Safety, MOE Key Lab New Proc Technol Nonferrous Met & Mat, Key Lab High Performance Struct Mat & Thermo surfa, Nanning 530004, Guangxi, Peoples R China.;[Fujita, Toyohisa; Wang, Xinpeng; Gao, F; Li, Ao; Huang, Jiaming; Mi, Shuxin; Gao, Feng; Ren, Feng] Guangxi Univ, Coll Resources Environm & Mat, Nanning 530004, Peoples R China.;[Ning, Shunyan] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Gao, F ] G;Guangxi Univ, State Key Lab Featured Met Mat & Life Cycle Safety, MOE Key Lab New Proc Technol Nonferrous Met & Mat, Key Lab High Performance Struct Mat & Thermo surfa, Nanning 530004, Guangxi, Peoples R China.;Guangxi Univ, Coll Resources Environm & Mat, Nanning 530004, Peoples R China.
摘要：
The effects of different sintering temperatures on the structure and upconversion luminescent properties of Li+ doped Bi3.46Ho0.04Yb0.5Ti3O12: 0.05Li (BHYTO: Li) phosphors were investigated. The upconversion emission intensity first increases and then decreases with the increase of sintering temperature from 700 to 1000 degrees C. The temperature induced grain growth contributes to the enhancement of UC luminescence, but the increased defects such as Bi and O degrade the luminescence. The combined effect of them makes the optimal luminescence appears in the specimens sintered at 800 degrees C, which is about 2 times stronger than that of those specimens sintered at 700 and 1000 degrees C. The mean lifetime for the specimens sintered from 700 to 1000 degrees C increases monotonically from 60.2 to 81.5 mu s, with band gap (E-g) from 2.8 to 3.0 eV. The maximal quantum efficiency can reach 0.28 % due to enhanced crystallinity.
期刊：
JOURNAL OF MATERIALS CHEMISTRY B,2024年12(6):1530-1537 ISSN：2050-750X
通讯作者：
Cheng, D;He, Longwei
作者机构：
[Cheng, Dan; He, Longwei; He, LW; Jiang, Renfeng] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;[Cheng, Dan; Zhang, Hongshuai; Yang, Xuefeng] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Gastroenterol, Hengyang 421002, Hunan Prov, Peoples R China.;[Cheng, Dan; Liu, Qian; Zhang, Hongshuai; Yang, Xuefeng; Xia, Yuqing] Univ South China, Affiliated Nanhua Hosp, Hunan Prov Clin Res Ctr Metab Associated Fatty Liv, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.
通讯机构：
[Cheng, D ; He, LW] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Gastroenterol, Hengyang 421002, Hunan Prov, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hunan Prov Clin Res Ctr Metab Associated Fatty Liv, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421002, Hunan, Peoples R China.
摘要：
Carboxylesterases (CESs) are critical for metabolizing ester-containing biomolecules and are specifically important in liver metabolic disorders. The modulation of CESs is also an important issue in pharmacology and clinical applications. Herein, we present a near-infrared (NIR) CES fluorescent probe (NCES) based on the protection-deprotection of the hydroxyl group for monitoring CES levels in living systems. The NCES probe has good selectivity and sensitivity for CESs with a limit of detection (LOD) of 5.24 mU mL-1, which allows for tracing the fluctuation of cellular CES after treatment with anticancer drugs and under inflammation and apoptosis states. Furthermore, NCES can be successfully applied for guiding liver cancer surgery with high-contrast in vivo imaging and detecting clinical serum samples from liver cancer patients. This work showed that the NCES probe has great potential in drug development, imaging applications for medical diagnosis, and early-stage detection for clinical liver diseases. A carboxylesterase-activated near-infrared fluorescent probe with high sensitivity and selectivity was developed to guide surgical resection of liver tumors and monitor clinical serum samples from liver cancer patients.
作者机构：
[Meng-qi Jin; Kai Weng; Zhou-ping Guo; Deng-hui Chen; Xiao-jian Zhao] Xi'an Center,China Geological Survey,Ministry of Natural Resources,Xi'an 710054,China;[Xiao-jian Zhao] Xinjiang Research Center for Mineral Resources,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;[Xiao-jian Zhao] College of Geography and Remote Sensing Sciences,Xinjiang University,Urumqi 830046,China;[Xiao-jian Zhao] University of Chinese Academy of Sciences,Beijing 100029,China;[Ming Wang] No.8 Geological Party,Xinjiang Bureau of Geology and Mineral Exploration and Development,Aksu 843000,China
关键词：
strata;Triassic;Xinjiang
摘要：
1. Objective
In the past decade, a group of medium to giant lead-zinc deposits, represented by Huoshaoyun, Sachakou, and Yuanbaoling, have been discovered in the Aksai Chin region of Karakoram, Xinjiang. They are all located in the Mesozoic carbonate and clastic rock formations. The Sachakou lead-zinc mining area is adjacent to the northwest of the Huoshaoyun lead-zinc mining area and is in the same stratigraphic layer as Huoshaoyun. Although many scholars have been arguing about the type and age of Huoshaoyun lead-zinc mineralization, few scholars have paid attention to the classification of the ore-bearing strata in the area. The stratigraphy of the Lower Permian Shenxianwan Group to the Upper Cretaceous Tielongtan Group is exposed in the Sachakou area of Karakorum, Xinjiang, however, the Late Permian-Early Triassic stratigraphy is missing (Fig. 1a). Due to the harsh natural conditions in the area and the low level of work, the stratigraphic delineation is not exhaustive, and the regional lithology is dominated by carbonates and clastic rocks, which makes it difficult to identify the age of the regional lithology and causes problems for the exploration and research of lead-zinc in the area.
摘要：
Altered gut microbiota and metabolites are important for non-alcoholic fatty liver disease (NAFLD) in children. We aimed to comprehensively examine the effects of gut metabolites on NAFLD progression. We performed integrative metabolomics (untargeted discovery and targeted validation) analysis of non-alcoholic fatty liver (NAFL), non-alcoholic steatohepatitis (NASH), and obesity in children. Fecal samples were collected from 75 subjects in the discovery cohort (25 NAFL, 25 NASH, and 25 obese control children) and 145 subjects in an independent validation cohort (53 NAFL, 39 NASH, and 53 obese control children). Among 2,491 metabolites, untargeted metabolomics revealed a complete NAFLD metabolic map containing 318 increased and 123 decreased metabolites. Then, machine learning selected 65 important metabolites that can distinguish the severity of the NAFLD. Furthermore, precision-targeted metabolomics selected 5 novel gut metabolites from 20 typical metabolites. The functionality of candidate metabolites was validated in hepatocyte cell lines. In the end, this study annotated two novel elevated pathogenic metabolites (dodecanoic acid and creatinine) and a relationship between depleted protective gut microbiota (Butyricicoccus and Alistipes), increased inflammation (IL-1 beta), lipid metabolism (TG), and liver function (ALT and AST). This study demonstrates the role of novel gut metabolites (dodecanoic acid and creatinine), as the fatty acid metabolism regulator contributing to NAFLD development through its influence on inflammation and liver function.IMPORTANCEAltered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota (Butyricicoccus and Alistipes) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development. Altered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota (Butyricicoccus and Alistipes) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development.
摘要：
Electrochemical conversion of nitrate offers an efficient solution to nitrate pollution and a sustainable strategy for ammonia generation. Cu and Fe bimetallic electrocatalysts exhibit excellent electrochemical reduction of nitrate (NO3RR) reactivity but the conventional preparation strategy is complex and time-consuming and this reaction is still suffers from unsatisfied kinetic and unidentified mechanisms. Herein, in situ electrodeposition strategy is employed to induce Cu to modify the Fe active sites of iron-based N-doping carbon nanofiber electrode (Fe/Fe3C@NCNFs) during NO3RR in Cu-contained nitrate solution. Benefiting from the synergistic effect between Cu and Fe sites of CuFe/Fe3C@NCNFs electrode, superior activity of rate-determining reaction (*NO(3 )to *NO2) and reduced energy barriers of the following deoxidation and hydrogenation steps are achieved. Compared with Fe/Fe3C@NCNFs-500, the pseudo-first-order (PFO) rate constant for NO3RR by CuFe/Fe3C@NCNFs demonstrates nearly two-fold improvement with high current efficiencies over wide pH and voltage range. Furthermore, the maximum NO3N removal capacity and N-2 selectivity of CuFe/Fe3C@NCNFs reach 15593.8 mg N g(-1) Fe and ca. 92% after twenty cycles. This work offers an avenue for highly active bimetallic electrode design, paving more insights into the interactions between active site construction and NO3RR performance.
期刊：
Biological Trace Element Research,2024年 ISSN：0163-4984
通讯作者：
Peng, CY;Liao, HQ
作者机构：
[Peng, Cuiying; Wang, Die; Zhou, Cuilan; Yin, Dan; Mao, Rui; Yu, Ping; Liu, Jun; Li, Suyun] Univ South China, Inst Cytol & Genet,Key Lab Typ Environm Pollut & H, Sch Basic Med Sci,Dept Educ,Key Lab Hengyang City, Hengyang Med Sch,Key Lab Ecol Environm & Crit Huma, Hengyang 421001, Hunan, Peoples R China.;[Nie, Yulin; Liao, Hongqing] Univ South China, Affiliated Hosp 2, Dept Obstet & Gynecol, 30 Jiefang Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, HQ ; Peng, CY ] U;Univ South China, Inst Cytol & Genet,Key Lab Typ Environm Pollut & H, Sch Basic Med Sci,Dept Educ,Key Lab Hengyang City, Hengyang Med Sch,Key Lab Ecol Environm & Crit Huma, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 2, Dept Obstet & Gynecol, 30 Jiefang Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
ART outcomes;Plasma metal levels;Polycystic ovary syndrome;in vitro fertilization
摘要：
The objective of this study is to explore the correlation of metal levels with assisted reproductive technology (ART) outcomes in polycystic ovary syndrome (PCOS) patients. The individuals were recruited who met the research criteria, only tubal factor or male infertility served as the control group (n = 40) and patient group was PCOS patients (n = 35). Individuals (n = 75) were divided into PCOS group (n = 35) and control group (n = 40). The normal body mass index (BMI) group (control) includes women with BMI < 25kg/m(2) in PCOS group (n = 24) and control group (n = 33), and BMI ≥ 25kg/m(2) in PCOS group (n = 11) and control group (n = 7). We performed an analysis of insulin resistance (IR) (n = 15) group and without insulin resistance (NIR) group (n = 20) in PCOS patient and control patients. Comparing difference demographic data, ART outcomes and the metal levels in every group respectively, the correlation of metal levels and ART outcomes in control participants and PCOS patients were analyzed by the Spearman correlation analysis, and multiple linear regression model was used to examine the association between the concentration of 19 metals and ART outcomes in PCOS group and control group. Plasma manganese (Mn), titanium (Ti), sodium (Na), magnesium (Mg), copper (Cu), calcium (Ca)/Mg ratio, and Cu/zinc (Zn) ratio levels in PCOS patients were higher than that in control, while Zn and Ca levels were lower in PCOS patients than that in control. The Mg levels had a positive connection with the number of eggs recovered, and the iron (Fe) levels were positively associated with the number of transplanted embryos in PCOS-IR. In PCOS-NIR, Mn levels positively correlated with the number of follicles and the number of good embryos. Silver (Ag) levels were negatively correlated with the number of follicles, and aluminum (Al) levels were negatively related with the normal fertilization and the number of good embryos. The Spearman analysis in PCOS-BMI ≥ 25 group exhibited that nickel (Ni) levels were negatively associated with the number of follicles. The plasma metal levels seem to affect the clinical manifestations and in vitro fertilization outcomes in assisted reproduction.
摘要：
The super tau-charm facility (STCF) is an electron-positron collider proposed by the Chinese particle physics community. It is designed to operate in a center-of-mass energy range from 2 to 7 GeV with a peak luminosity of 0.5 x 1035 cm-2 center dot s-1 or higher. The STCF will produce a data sample about a factor of 100 larger than that of the present tau-charm factory - the BEPCII, providing a unique platform for exploring the asymmetry of matter-antimatter (charge-parity violation), in-depth studies of the internal structure of hadrons and the nature of non-perturbative strong interactions, as well as searching for exotic hadrons and physics beyond the Standard Model. The STCF project in China is under development with an extensive R&D program. This document presents the physics opportunities at the STCF, describes conceptual designs of the STCF detector system, and discusses future plans for detector R&D and physics case studies.
摘要：
BACKGROUND: Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) has been demonstrated to play vital roles in development and progression of tumors such as gastric cancer (GC). However, the detailed molecular mechanism of OIP5-AS1 has not been completely elucidated. Our study aimed to investigate the role and the epigenetic regulation mechanism of OIP5-AS1 in GC. METHODS: OIP5-AS1 expression in GC tissues was detected by RT-qPCR. Loss- and gain-of-function experiments were conducted to assess the biological function of OIP5-AS1 in vitro and in vivo. The interaction of OIP5-AS1 with insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) or heterogeneous nuclear nucleoprotein A1 (hnRNPA1) was verified by bioinformatics analysis, RNA pull-down assays, and RNA immunoprecipitation assays. RESULTS: In this study, we identified that OIP5-AS1 is specifically overexpressed in GC tumor tissues and cell lines and correlated with a poor prognosis. The loss of OIP5-AS1 suppressed the proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and glycolysis of GC cells, but the ectopic expression of OIP5-AS1 had the opposite impact. Meanwhile, knockdown of OIP5-AS1 inhibited tumor growth in patient-derived xenograft models, as well as repressed tumor metastasis. Mechanistically, IGF2BP3 could bind to OIP5-AS1 by N6-methyladenosine (m6A) modification sites on OIP5-AS1, thereby stabilizing OIP5-AS1. Moreover, OIP5-AS1 prevented Trim21-mediated ubiquitination and degradation of hnRNPA1, stabilizing hnRNPA1 protein and promoting the malignant progression of GC by regulating PKM2 signaling pathway. CONCLUSIONS: In conclusion, this study highlighted that OIP5-AS1 is an oncogenic m6A-modified long non-coding RNA (lncRNA) in GC and that IGF2BP3/OIP5-AS1/hnRNPA1 axis may provide a potential diagnostic or prognostic target for GC.
摘要：
In this paper, nitrogen-doped carbon dots (NCDs) were synthesized via a facile hydrothermal method using gum arabic (GA) and tris(hydroxymethyl)methyl aminomethane (tris) as precursors. The resultant NCDs show uniform size distribution and stable optical performance. Then a fluorescent signal quenching probe based on NCDs was proposed for the quantitative detection of mercury ions (Hg2+). The developed fluorescent nanoprobe based on NCDs demonstrated a good linear relationship in the range of 10 to 120 mu M (R-2 = 0.99265) for toxic mercury(ii), with a detection limit of 4.87 mu M. Additionally, the present assay system could be efficiently used for the detection of Hg2+ in real water samples with a satisfactory recovery rate (96.11-104.04%). The metal-free NCDs, which exhibited little cytotoxicity, were successfully applied to in vitro fluorescence imaging of human triple negative breast cancer (TNBC) cells, achieving visualization of Hg2+ in cells. With good water solubility and biocompatibility, the nitrogen-doped carbon dots synthesized by this economical and ecologically friendly process are suitable for use in analytical testing and biological research.
期刊：
MINI-REVIEWS IN MEDICINAL CHEMISTRY,2024年24(13):1228-1237 ISSN：1389-5575
作者机构：
Hunan University of Chinese Medicine & Hunan Engineering Technology Center of Standardization and Function of
Chinese Herbal Decoction Pieces, Changsha, 410208, Hunan, China;State Key Laboratory of Bioactive Substances and
Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences
and Peking Union Medical College, Beijing, 100050, China;Shanxi Key Laboratory of Traditional Chinese
Medicine Encephalopathy, Shanxi University of Traditional Chinese Medicine, Taiyuan, 030024, China;Research lab of translational medicine, Hengyang Medical
College, University of South China, Hengyang, Hunan 421001, China;[Feng, Juling] Hunan University of Chinese Medicine & Hunan Engineering Technology Center of Standardization and Function of
Chinese Herbal Decoction Pieces, Changsha, 410208, Hunan, China <&wdkj&> Research lab of translational medicine, Hengyang Medical
College, University of South China, Hengyang, Hunan 421001, China
摘要：
Ginsenoside is the principal active ingredient in ginseng. Several investigations have found that ginsenosides have anti-inflammatory, antioxidant, anti-apoptotic, anti-cancer, and antiallergic activities. Ferroptosis is an iron-dependent, non-apoptotic form of cell-regulated death caused by lipid peroxidation. Iron, lipid, and amino acid metabolism orchestrate the complex ferroptosis response through direct or indirect regulation of iron accumulation or lipid peroxidation. More and more research has demonstrated that ginsenoside impacts illnesses via ferroptosis, implying that ferroptosis might be employed as a novel target of ginsenoside for disease therapy. This article examines the molecular mechanism of ferroptosis as well as the current advancement of ginsenoside in influencing disorders via ferroptosis.
摘要：
Uranium contamination is a key issue in the sustainable development of nuclear energy. In this study, a cellulose/sericite hybrid aerogel with a layer-stacked network structure (MCC/AS-P) was prepared for uranium-contaminated wastewater treatment. Systematic characterization and multiple-batch static adsorption experiments were conducted to analyze the aerogel's preparation, adsorption, and desorption. The kinetics demonstrated a noticeable transition between mass transfer diffusion control and mass transfer control, approaching adsorption equilibrium within 8 min and 180 min, respectively, wherein polymer layers led to a more stable adsorption process. Adsorption isotherm and thermodynamic studies established that the theoretical adsorption capacity of MCC/AS-P for U(VI) at T = 298 K could reach 374.5 mg.g(-1). The adsorption behavior was endothermic and spontaneous, and the DFT calculations demonstrated that the adsorption energy of MCC/AS for UO22+ was 506.5 kcal/mol. Temperature, U(VI) concentration, and desorption can all lead to a transition of the dominant mechanism between chemisorption and physisorption. After six swelling-deswelling adsorption cycles, the adsorption efficiency remained above 80%, and the structure remained intact. Furthermore, the excellent performance in terms of interference resistance and chemical stability offers potential for practical application.
