期刊论文

Effects of dapagliflozin on ischemic ventricular arrhythmia in rats by regulating JAK2/STAT3 axis

中文

中国临床药理学杂志

1001-6821

2024

40

3

388-392

10.13699/j.cnki.1001-6821.2024.03.018

2021JJ30608:湖南省自然科学基金资助项目

本校为第一机构

目的 探讨达格列净(DAPA)调控蛋白酪氨酸激酶2(JAK2)/信号转导子与激活子3(STAT3)轴对大鼠缺血性室性心律失常(VA)的作用及机制研究.方法 将大鼠随机分为假手术组、模型组和低、中、高剂量实验组及AG490组,每组10只.除假手术组外各组用结扎冠状动脉左前降支(LAD)构建大鼠缺血性VA模型.造模后48 h,低、中、高剂量实验组分别给予大鼠1、3、5 mg·kg-1 DAPA 灌胃,AG490 组灌胃 5 mg·kg-1 DAPA+5 mg·kg1 AG490,每日 1 次,持续30 d.用心电图检查大鼠心律失常评分,用蛋白质印迹法检测心肌组织中磷酸化 JAK2(p-JAK2)/JAK2 和磷酸化 STAT3(p-STAT3)/STAT3 蛋白的表达水平.结果 假手术组、模型组、低、...

Objective To investigate the effect and mechanism of dapagliflozin(DAPA)on ischemic ventricular arrhythmia(VA)in rats by regulating the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)axis.Methods The rats were randomly divided into sham operation group,model group,experimental-L,-M,-H groups and AG490 group.All groups except sham group were established model of ischemic VA by ligation of the left anterior descending coronary artery(LAD).After 48 h of modeling,the experimental-L,-M,-H groups were given 1,3,5 mg·kg...