作者机构：
[He, Chunlin; Liu, Zunzhang; Fujita, Toyohisa; Wang, Guifang; He, CL; Yang, Wenchao; Ma, Shaojian; Qi, Mingwei; Liu, Yun] Guangxi Univ, Sch Resources Environm & Mat, Nanning 530004, Peoples R China.;[He, Chunlin; Liu, Zunzhang; Fujita, Toyohisa; Wang, Guifang; He, CL; Yang, Wenchao; Ma, Shaojian; Qi, Mingwei; Liu, Yun] Guangxi Univ, State Key Lab Featured Met Mat & Life cycle Safety, Nanning 530004, Peoples R China.;[Wei, Yuezhou] Univ South China, Sch Nucl Sci & Technol, Hengyang 421000, Peoples R China.;[Wei, Yuezhou] Shanghai Jiao Tong Univ, Sch Nucl Sci & Engn, Shanghai 200240, Peoples R China.;[He, Chunlin; He, CL] Educ Dept Guangxi Zhuang Autonomous Reg, Key Lab New Low Carbon Green Chem Technol, Nanning 530004, Peoples R China.
通讯机构：
[He, CL ] G;Guangxi Univ, Sch Resources Environm & Mat, Nanning 530004, Peoples R China.;Guangxi Univ, State Key Lab Featured Met Mat & Life cycle Safety, Nanning 530004, Peoples R China.;Educ Dept Guangxi Zhuang Autonomous Reg, Key Lab New Low Carbon Green Chem Technol, Nanning 530004, Peoples R China.
关键词：
waste amidoxime resin;mesoporous adsorbent;H3PO4 and ZnCl2 processing;Cr(VI) adsorption;electroplating wastewater
摘要：
With the application of resins in various fields, numerous waste resins that are difficult to treat have been produced. The industrial wastewater containing Cr(VI) has severely polluted soil and groundwater environments, thereby endangering human health. Therefore, in this paper, a novel functionalized mesoporous adsorbent PPR-Z was synthesized from waste amidoxime resin for adsorbing Cr(VI). The waste amidoxime resin was first modified with H3PO4 and ZnCl2, and subsequently, it was carbonized through slow thermal decomposition. The static adsorption of PPR-Z conforms to the pseudo-second-order kinetic model and Langmuir isotherm, indicating that the Cr(VI) adsorption by PPR-Z is mostly chemical adsorption and exhibits single-layer adsorption. The saturated adsorption capacity of the adsorbent for Cr(VI) could reach 255.86 mg/g. The adsorbent could effectively reduce Cr(VI) to Cr(III) and decrease the toxicity of Cr(VI) during adsorption. PPR-Z exhibited Cr(VI) selectivity in electroplating wastewater. The main mechanisms involved in the Cr(VI) adsorption are the chemical reduction of Cr(VI) into Cr(III) and electrostatic and coordination interactions. Preparation of PPR-Z not only solves the problem of waste resin treatment but also effectively controls Cr(VI) pollution and realizes the concept of "treating waste with waste".
期刊：
QUANTUM INFORMATION PROCESSING,2024年23(4):1-20 ISSN：1570-0755
通讯作者：
Songfeng Lu
作者机构：
[Hao Yang; Qing Zhou] Hubei Key Laboratory of Distributed System Security, Hubei Engineering Research Center on Big Data Security, School of Cyber Science and Engineering, Huazhong University of Science and Technology, Wuhan, China;Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, China;[Mu Wang] The NanHua Affiliated Hospital, Clinical Research Institute, Hengyang Medical School, University of South China, Hengyang, China;[Bingyan Feng] Industrial Internet Research Institute, Wuhan Huazhong Numerical Control Co., Ltd., Wuhan, China;[Xianjing Zhou] Wuhan Zhuoer Information Technology Co., Ltd., Wuhan, China
通讯机构：
[Songfeng Lu] H;Hubei Key Laboratory of Distributed System Security, Hubei Engineering Research Center on Big Data Security, School of Cyber Science and Engineering, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, China
关键词：
Quantum cryptography;Multi-party quantum key agreement;Maximally entangled state
摘要：
In this paper, we proposed an efficient single-state three-party quantum key agreement (QKA) protocol. We proved that the protocol can resist potential outside attacks and inside attacks, and we generalized the efficient single-state three-party QKA scheme into the case of the N-party by substituting N-particle entangled state for three-particle entangled state as the resource quantum states. Compared with the previous QKA protocols, our scheme contains the good features of previous schemes, i.e., using one kind of maximally entangled states as the quantum resource, no requirements for pre-shared key between different participants, and no requirements for executing any unitary operations or quantum entanglement swapping. Furthermore, our scheme has significant improvements in terms of the times of quantum states transmission, the consumed qubits, and the qubit efficiency. In particular, as the number of participants increases, the number of qubits required by our scheme increases linearly rather than exponentially.
期刊：
Journal of Hazardous Materials,2024年469:134022 ISSN：0304-3894
通讯作者：
Xue, Wenjing
作者机构：
[Lin, Weilong; Liu, Hongdou; Wen, Siqi; He, Qi; Shi, Xiaoyu; Xu, Yiqun; Guo, Jiaming] College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, PR China;[Gao, Yang] School of Hydraulic and Environmental Engineering, Changsha University of Science & Technology, Changsha 410114, PR China;[Wang, Rongzhong] School of Resource & Environment and Safety Engineering, University of South China, Hengyang 421001, PR China;[Xue, Wenjing] College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, PR China. Electronic address: xuewenjing@yzu.edu.cn
通讯机构：
[Xue, Wenjing] C;College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, PR China. Electronic address:
关键词：
Bacterial community;Cadmium;Precursor;Sediment;Sulfidized nanoscale zero-valent iron
摘要：
Sulfidized nanoscale zero-valent iron (S-nZVI) showed excellent removal capacity for cadmium (Cd) in aqueous phase. However, the remediation effects of S-nZVI on Cd-contaminated sediment and its interactions with microorganisms in relation to Cd fate remain unclear. The complexity of the external environment posed a challenge for Cd remediation. This study synthesized S-nZVI with different S and Fe precursors to investigate the effect of precursors and applied the optimal material to immobilize Cd in sediments. Characterization analysis revealed that the precursor affected the morphology, Fe(0) crystallinity, and the degree of oxidation of the material. Incubation experiments demonstrated that the immobilization efficiency of Cd using S-nZVI(Fe)(3+)(+S)(2-) (S/Fe = 0.14) reached the peak value of 99.54%. 1% and 5% dosages of S-nZVI significantly reduced Cd concentration in the overlying water, DTPA-extractable Cd content, and exchangeable (EX) Cd speciation (P<0.05). Cd leaching in sediment and total iron in the overlying water remained at low levels during 90 d of incubation. Notably, each treatment maintained a high Cd immobilization efficiency under different pH, water/sediment ratio, organic acid, and coexisting ion conditions. Sediment physicochemical properties, functional bacteria, and a range of adsorption, complexation and precipitation of CdS effects dominated Cd immobilization.
期刊：
Case Studies in Thermal Engineering,2024年53:103820 ISSN：2214-157X
通讯作者：
He, Danqiu;Isa, MHM
作者机构：
[Isa, Mohd Hafizal Mohd; Isa, MHM; He, Danqiu; He, DQ] Univ Sains Malaysia, Sch Housing Bldg & Planning, George Town 11800, Malaysia.;[He, Danqiu] Univ South China, Solux Coll Architecture & Design, Hengyang 421001, Peoples R China.
通讯机构：
[Isa, MHM ; He, DQ] U;Univ Sains Malaysia, Sch Housing Bldg & Planning, George Town 11800, Malaysia.
关键词：
Indoor thermal comfort;Heritage building;Neutral temperature;Sustainable development
摘要：
The heritage buildings of a country or region are frequently considered to be of value and sig-nificance to the current generation. This study investigates the winter indoor thermal comfort of heritage buildings in hot summer and cold winter zone of China. Field measurements and questionnaire surveys were used to conduct specific studies on the winter indoor thermal comfort of two typical traditional residential buildings ("Yinziwu" and "Diaojiaolou") in the Yuan River basin heritage building area. The aim is to investigate the current status of building thermal comfort and provide a theoretical basis for promoting their sustainable development. The results show that 1) Indoor air temperatures in both types of buildings are significantly and positively correlated in winter, but both are below the human thermal comfort range. 2) Mean air temperatures measured in "Yinziwu" and "Diaojiaolou" are 8.8 degrees C and 9.4 degrees C, respectively, with neutral air temperatures of 11.1 degrees C and 13.8 degrees C. 3) Thermal sensation, thermal preference, and thermal comfort were significantly correlated in the two buildings, with ooccupants experiencing the coldest phase from 7:00-11:00 and the warmest phase from 15:00-19:00 daily. 4) "Yinziwu" occupants are more tolerant of the cold climate.
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2024年14:1332666 ISSN：2235-2988
通讯作者：
Liu, YX
作者机构：
[Ye, Guoguo; Liu, Yingxia; Li, Yanjie; Qing, Ling; Zhang, Li; Yang, Liuqing; Ou, Guanyong; Peng, Ling; Yang, Yang] Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, State Key Discipline Infect Dis,Hosp 2, Shenzhen, Peoples R China.;[Ou, Guanyong] Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China.;[Qing, Ling; Zhang, Li] Univ South China, Grad Collaborat Training Base, Hengyang Med Sch, Shenzhen Peoples Hosp 3, Hengyang, Peoples R China.
通讯机构：
[Liu, YX ] S;Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, State Key Discipline Infect Dis,Hosp 2, Shenzhen, Peoples R China.
关键词：
hepatitis B vaccine;HGF;IL-5;cytokine;immune response;infant;non/low response
摘要：
BACKGROUND: The immune response to hepatitis B vaccine may be influenced by numerous factors, and patients with non/low response re-exposed to hepatitis B virus remain susceptible. Thus, a better understanding of the underlying mechanisms of non/low immune response in infants born to Hepatitis B surface antigen (HBsAg)-positive mothers is essential. METHODS: 100 infants born to HBsAg-positive mothers from 2015 to 2020 were enrolled in the study, further divided into the non/low response group (n=13) and the moderate strong response group (n=87) based on the quantification of hepatitis B surface antibody at 12 months of age. The differential expression of 48 immune-related cytokines in the two groups was compared and analyzed in detail. The key cytokines were further identified and clinically predictive models were developed. RESULTS: We found that 13 cytokines were lowly expressed and one cytokine was highly expressed in the non/low response group, compared with the moderate strong response group at birth. In addition, 9 cytokines were lowly expressed and one cytokine was highly expressed in the non/low response group at 12 months of age. Furthermore, we found that IL-5 and HGF were promising predictors for predicting the immunization response to hepatitis B vaccine in infants, and the combination of the two cytokines showed the best predictive efficiency, with an area under the curve (AUC) value of 0.844. CONCLUSION: The present study provides a theoretical basis on cytokines for developing and implementing effective immunotherapies against non/low immune response in infants born to HBsAg-positive mothers.
作者机构：
[Cheng, Pin-Jing; Tang, Lei-Xun; Zheng, Bo; Feng, Song; Wang, Hao-Qiang; Li, Xin-Xiang; Zhao, Ji-Rong; Zhao, Da-Jun; Xue, Jie-Ming; Xiao, Min; Luo, Wen; Li, Bao-Qian] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Yi, Han; Chen, Yong-Hao] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China.;[Yi, Han; Chen, Yong-Hao] Spallat Neutron Source Sci Ctr, Dongguan 523803, Peoples R China.;[Liu, Rong; Yang, Yi-Wei; Han, Zi-Jie] China Acad Engn Phys, Inst Nucl Phys & Chem, Mianyang 621900, Peoples R China.
通讯机构：
[Feng, S ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
关键词：
Neutron-induced total cross section;Natural lead;Time-of-flight;TALYS-1.96;CSNS Back-n white neutron beamline
摘要：
The neutron-induced total cross sections of natural lead have been measured in a wide energy range (0.3 eV–20 MeV) on the back-streaming white neutron beamline (Back-n) at the China Spallation Neutron Source. Neutron energy was determined by the neutron total cross-section spectrometer using the time-of-flight technique. A fast multi-cell fission chamber was used as the neutron detector, and a 10-mm-thick high-purity natural lead sample was employed for the neutron transmission measurements. The on-beam background was determined using Co, In, Ag, and Cd filters. The excitation function of
$$^\text {nat}$$
Pb(n, tot) reaction below 20 MeV was calculated using the TALYS-1.96 nuclear-reaction modeling program. The present results were compared with previous results, the evaluated data available in the five major evaluated nuclear data libraries (i.e., ENDF/B-VIII.0, JEFF-3.3, JENDL-5, CENDL-3.2, and BROND-3.1), and the theoretical calculation curve. Good agreement was found between the new results and those of previous experiments and with the theoretical curves in the corresponding region. This measurement obtained the neutron total cross section of natural lead with good accuracy over a wide energy range and added experimental data in the resonance energy range. This provides more reliable experimental data for nuclear engineering design and nuclear data evaluation of lead.
作者机构：
[Fang, Hongyi; Ke, Weijun; Pu, Dexin; Guan, Hongling; Zhou, Shun; Chen, Guoyi; Fang, Guojia; Fu, Shiqiang; Ke, WJ; Jia, Peng; Wang, Chen] Wuhan Univ, Sch Phys & Technol, Key Lab Artificial Micro & Nanostruct, Minist Educ, Wuhan 430072, Peoples R China.;[Jumabaev, Abduvakhid; Hushvaktov, Hakim] Samarkand State Univ, 15 Univ Blvd, Samarkand 140104, Uzbekistan.;[Meng, Weiwei] South China Normal Univ, South China Acad Adv Optoelect, Guangzhou 510006, Peoples R China.;[Wang, Xingzhu; Wang, XZ] Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China.;[Wang, Xingzhu; Wang, XZ] Univ South China, Sch Elect Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Meng, WW ; Wang, XZ ; Jumabaev, A ] S;[Ke, WJ ] W;Wuhan Univ, Sch Phys & Technol, Key Lab Artificial Micro & Nanostruct, Minist Educ, Wuhan 430072, Peoples R China.;Samarkand State Univ, 15 Univ Blvd, Samarkand 140104, Uzbekistan.;South China Normal Univ, South China Acad Adv Optoelect, Guangzhou 510006, Peoples R China.
关键词：
blade coating;large area;strain;tandem solar cells;wide-bandgap perovskites
摘要：
Blade‐coated wide‐bandgap perovskites encounter top–down inhomogeneity strains. Utilizing mixed‐cation post‐treatment for strain relief, large‐area wide‐bandgap solar cells demonstrate enhanced efficiency and stability. Specifically, 1 cm2‐area 1.77 eV‐bandgap cells achieve an 18.71% efficiency (stabilized at 18.50%), while 4‐terminal all‐perovskite tandems reach an exceptional 27.64% efficiency, coupled with enhanced stability. Abstract The realization of efficient large‐area perovskite solar cells stands as a pivotal milestone for propelling their future commercial viability. However, the upscaling fabrication of perovskite solar cells is hampered by efficiency losses, and the underlying growth mechanism remains enigmatic. Here, it is unveiled that a prevalent upscaling technology, namely blade‐coating, inherently triggers top‐down inhomogeneity strains, predominantly concentrated on the surface of wide‐bandgap perovskite films. Through strain mitigation strategies, the perovskite films exhibit reduced halide vacancies, leading to enhanced stability and improved optoelectronic characteristics. Consequently, the blade‐coated perovskite solar cells achieve minimal efficiency loss when transitioning from small‐area to large‐area devices, enabling the realization of 1 cm2‐area 1.77 eV‐bandgap cells with a remarkable efficiency of 18.71%. Additionally, the strain‐relieved device exhibits an exceptional 109% retention of its initial efficiency even after 400 h of continuous operation, in stark contrast to the control device which experiences a decline to 91%. Furthermore, the resulting 4‐terminal all‐perovskite tandem solar cells crafted utilizing blade‐coated 1.77 eV‐bandgap subcells achieve a maximum efficiency of 27.64% (stabilized at 27.28%). This study not only sheds light on the intricacies of upscaling preparation techniques but also overcomes potential obstacles that can impede the trajectory toward achieving large‐scale perovskite solar cells.
摘要：
小型棒控压水堆舍弃了可溶硼,并高度依赖控制棒与可燃毒物棒控制堆芯的反应性.为研究控制棒对堆芯关键性能的影响,本文以核动力破冰船用KLT-40模型为对象,以轴向功率偏移、堆芯寿期、燃料利用率与径向功率峰因子为指标,开展长寿期小型棒控压水堆控制棒布置与动作策略设计分析.首先,基于OpenMC程序开发带棒燃耗程序;其次,比较堆芯带控制棒与无控制棒运行时的堆芯寿期等指标;最后,分析不同动作策略对轴向功率偏移等指标的影响.结果表明:控制棒将堆芯寿期从590 EFPDs(等效满功率天,Effective full power days)延长至650～698 EFPDs;低价值棒组优先动作策略使轴向功率偏移程度由-0.69与+0.80分别下降至-0.29与+0.52.因此,要准确计算长寿期压水堆寿期必须采用带控制棒燃耗计算策略,并且通过合理的动作策略能够有效减小控制棒带来的轴向功率偏移.
作者机构：
[Zhu, Lin; Tan, Xian; He, Yechuan; Wu, Xiaoqi; Wang, Danling] Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Zhu, Lin; Tan, Xian; He, Yechuan; Wu, Xiaoqi; Wang, Danling] Univ South China, Inst Future Sci, Changsha 410008, Peoples R China.;[Jiang, Binyuan] Univ South China, Affiliated Changsha Cent Hosp, Med Res Ctr, Hengyang Med Sch, Changsha 410004, Peoples R China.;[Jiang, Binyuan; Yuan, Yeqin; Wang, Danling] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Clin Lab, Changsha 410004, Peoples R China.
通讯机构：
[Jiang, BY ] U;Univ South China, Affiliated Changsha Cent Hosp, Med Res Ctr, Hengyang Med Sch, Changsha 410004, Peoples R China.;Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Clin Lab, Changsha 410004, Peoples R China.
摘要：
Background: Nasopharyngeal carcinoma (NPC) exhibits a significant prevalence in the southern regions of China, and paclitaxel (PTX) is frequently employed as a medication for managing advanced NPC. However, drug resistance is typically accompanied by a poor prognosis. Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy. Methods: This study investigated whether 3-Methyladenine (3 -MA) could potentiated the effect of PTX and its potential molecular mechanism. Samples were divided into the following categories: Negative control (NC) with the solvent dimethyl sulfoxide (DMSO, 0.5% v/v), PTX (400 nM), 3 -MA (4 mM), and PTX (400 nM) + 3 -MA (4 mM). The viability of NPC cells was assessed using both the cell counting kit-8 (CCK-8) assay and the colony formation assay. Microscopic observation was performed to identify morphological cell changes. Flow cytometry was used to assess cell cycle status, mitochondrial membrane potential (MMP), and apoptotic cells. Western blotting was conducted to quantify the protein expression. Results: 3 -MA enhanced PTX-specific inhibition of NPC cell proliferation. PTX, either alone or in combination with 3 -MA, caused cell cycle halt at the G2/M phase in the majority of NPC cells, and the combination treatment of PTX with 3 -MA induced a higher rate of NPC cell death compared to PTX alone. Western blotting results revealed the combination of PTX with 3 -MA heightened activation of cyclin-dependent kinase 1 (CDK1), a key molecule in shifting cells from mitotic arrest to apoptosis, led to a reduction in Myeloid Cell Leukemia 1 (MCL-1) expression and an increase in Poly (ADP-ribose) polymerase (PARP) cleavage. Conclusion: The concurrent administration of PTX with 3 -MA effectively enhances PTX's inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.
作者：
Saito, Suguru*;Okuno, Alato;Peng, Zhenzi;Cao, Duo-Yao;Tsuji, Noriko M.
期刊：
FRONTIERS IN IMMUNOLOGY,2024年15 ISSN：1664-3224
通讯作者：
Saito, Suguru;Tsuji, NM;Saito, S
作者机构：
[Okuno, Alato; Tsuji, NM; Saito, Suguru; Tsuji, Noriko M.; Saito, S] Natl Inst Adv Ind Sci & Technol, Dept Life Technol & Sci, Div Cellular & Mol Engn, Tsukuba, Ibaraki, Japan.;[Saito, S; Saito, Suguru] Jichi Med Univ, Fac Med, Dept Infect & Immun, Div Virol, Shimotsuke, Tochigi, Japan.;[Cao, Duo-Yao; Saito, S; Saito, Suguru] Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA.;[Okuno, Alato] Shibata Gakuen Univ, Fac Human Design, Dept Hlth & Nutr, Hirosaki, Aomori, Japan.;[Peng, Zhenzi] Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Dept Cell Biol & Genet, Hengyang, Hunan, Peoples R China.
通讯机构：
[Saito, S ] J;[Tsuji, NM ; Saito, S] N;Natl Inst Adv Ind Sci & Technol, Dept Life Technol & Sci, Div Cellular & Mol Engn, Tsukuba, Ibaraki, Japan.;Jichi Med Univ, Fac Med, Dept Infect & Immun, Div Virol, Shimotsuke, Tochigi, Japan.;Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA.
关键词：
probiotics;lactic acid bacteria;dendritic cells;CD8+T cells;anti-tumor immunity;MHC class I;Immunoproteasome
摘要：
Lactic acid bacteria (LAB) possess the ability to argument T cell activity through functional modification of antigen presenting cells (APCs), such as dendritic cells (DCs) and macrophages. Nevertheless, the precise mechanism underlying LAB-induced enhancement of antigen presentation in APCs remains incompletely understood. To address this question, we investigated the detailed mechanism underlying the enhancement of major histocompatibility complex (MHC) class I-restricted antigen presentation in DCs using a probiotic strain known as Lactococcus lactis subsp. Cremoris C60. We found that Heat-killed-C60 (HK-C60) facilitated the processing and presentation of ovalbumin (OVA) peptide antigen OVA257-264 (SIINFEKL) via H-2Kb in bone marrow-derived dendritic cells (BMDCs), leading to increased generation of effector CD8+ T cells both in vitro and in vivo. We also revealed that HK-C60 stimulation augmented the activity of 20S immunoproteasome (20SI) in BMDCs, thereby enhancing the MHC class I-restricted antigen presentation machinery. Furthermore, we assessed the impact of HK-C60 on CD8+ T cell activation in an OVA-expressing B16-F10 murine melanoma model. Oral administration of HK-C60 significantly attenuated tumor growth compared to control treatment. Enhanced Ag processing and presentation machineries in DCs from both Peyer's Patches (PPs) and lymph nodes (LNs) resulted in an increased tumor antigen specific CD8+ T cells. These findings shed new light on the role of LAB in MHC class-I restricted antigen presentation and activation of CD8+ T cells through functional modification of DCs.
期刊：
Brain and Behavior,2024年14(2):e3373- ISSN：2162-3279
通讯作者：
Chen, YJ
作者机构：
[Chen, Liang; Chen, Yongjun; Xie, Yangzhi] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Neurol, Hengyang 421001, Peoples R China.;[Chen, Jiacheng] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Intens Care Unit, Hengyang, Peoples R China.
通讯机构：
[Chen, YJ ] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Neurol, Hengyang 421001, Peoples R China.
关键词：
M1/M2 polarization;neuroinflammation;Parkinson's disease;regulatory T cell;vitamin D receptor
摘要：
We explored the therapeutic effects of vitamin receptor activation by calcitriol in a mouse model of PD. We found that calcitriol is potent to protect against neuroinflammation and neuronal degeneration by boosting Treg. Vitamin D receptor may be a therapeutic option for PD. Abstract Objective Vitamin D deficiency is a risk factor for Parkinson's disease (PD) and vitamin D supplementation robustly alleviates neurodegeneration in PD models. However, the mechanisms underlying this effect require further clarification. Current evidence suggests that harnessing regulatory T cells (Treg) may mitigate neuronal degeneration. In this study, we investigated the therapeutic effects of vitamin D receptor activation by calcitriol on PD, specifically focusing on its role in Treg. Methods Hemiparkinsonian mice model was established through the injection of 6‐OHDA into the striatum. Mice were pretreated with calcitriol before 6‐OHDA injection. The motor performance, dopaminergic neuronal survival, contents of dopamine, and dopamine metabolites were evaluated. The pro‐inflammatory cytokines levels, T‐cell infiltration, mRNA expression of indicated microglial M1/M2 phenotypic markers, and microglial marker in the midbrain were detected. Populations of Treg in the splenic tissues were assessed using a flow cytometry assay. PC61 monoclonal antibody was applied to deplete Treg in vivo. Results We show that calcitriol supplementation notably improved motor performance and reduced dopaminergic degeneration in the 6‐OHDA‐induced PD model. Mechanistically, calcitriol promoted anti‐inflammatory/neuroprotective Treg and inhibited pro‐inflammatory/neurodestructive effector T‐cell generation in this model. This process significantly inhibited T‐cell infiltration in the midbrain, restrained microglial activation, microglial M1 polarization, and decreased pro‐inflammatory cytokines release. This more favorable inflammatory microenvironment rescued dopaminergic degeneration. To further verify that the anti‐inflammatory effects of calcitriol are associated with Treg expansion, we applied an antibody‐mediated Treg depletion assay. As predicted, the anti‐inflammatory effects of calcitriol in the PD model were diminished following Treg depletion. Conclusion These findings suggest that calcitriol's anti‐inflammatory and neuroprotective effects in PD are associated with its potential to boost Treg expansion.
期刊：
Cancer Cell International,2024年24(1):1-21 ISSN：1475-2867
通讯作者：
Tan, Yeru;Li, YH
作者机构：
[Jiang, Baohong] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pharm, Hengyang, Hunan, Peoples R China.;[Zeng, Lijun; Tan, Yeru; Li, YH; Li, Yuehua; Tang, Yuanbin; Luo, Lunqi; Ouyang, Lianjie; Feng, Wenjie; Tan, YR; Wu, Sixuan] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Oncol, Hengyang, Hunan, Peoples R China.;[Wu, Sixuan] Fujian Med Univ, Fujian Canc Hosp, Clin Oncol Sch, Fuzhou, Fujian, Peoples R China.
通讯机构：
[Li, YH ; Tan, YR] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Oncol, Hengyang, Hunan, Peoples R China.
关键词：
Breast cancer;NDUFAF6;NRF2;PD-L1;Immune infiltration;Prognosis
摘要：
Breast cancer is a major global health concern, and there is a continuous search for novel biomarkers to predict its prognosis. The mitochondrial protein NDUFAF6, previously studied in liver cancer, is now being investigated for its role in breast cancer. This study aims to explore the expression and functional significance of NDUFAF6 in breast cancer using various databases and experimental models. We analyzed breast cancer samples from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and Human Protein Atlas (HPA) databases, supplemented with immunohistochemistry (IHC) staining to assess NDUFAF6 expression. A breast cancer cell xenograft mouse model was used to evaluate tumor growth, apoptosis, and NDUFAF6 expression. Survival probabilities were estimated through Kaplan–Meier plots and Cox regression analysis. A Protein–Protein Interaction (PPI) network was constructed, and differentially expressed genes related to NDUFAF6 were analyzed using GO, KEGG, and GSEA. The relationship between NDUFAF6 expression, immune checkpoints, and immune infiltration was also evaluated. NDUFAF6 was found to be overexpressed in breast cancer patients and in the xenograft mouse model. Its expression correlated with worse clinical features and prognosis. NDUFAF6 expression was an independent predictor of breast cancer outcomes in both univariate and multivariate analyses. Functionally, NDUFAF6 is implicated in several immune-related pathways. Crucially, NDUFAF6 expression correlated with various immune infiltrating cells and checkpoints, particularly promoting PD-L1 expression by inhibiting the NRF2 signaling pathway. The study establishes NDUFAF6 as a potential prognostic biomarker in breast cancer. Its mechanism of action, involving the inhibition of NRF2 to upregulate PD-L1, highlights its significance in the disease's progression and potential as a target for immunotherapy.
