作者： Wu, Runliu;Li, Liang;Bai, Yang;Yu, Bowen;Xie, Canbin;...

期刊： CELL DEATH & DISEASE,2020年11(10):1-13 ISSN：2041-4889

通讯作者： Li, Xiaorong;Lin, Changwei

作者机构： [Li, Xiaorong; Yu, Bowen; Wu, Hao; Wu, Runliu; Bai, Yang; Xie, Canbin; Zhang, Yi; Lin, Changwei] Cent South Univ, XiangYa Hosp 3, Dept Gastrointestinal Surg, Changsha 410013, Hunan, Peoples R China.;[Li, Liang] Univ South China, Sch Med, Five Year Program Clin Med, Hengyang 421001, Hunan, Peoples R China.;[Huang, Lihua] Cent South Univ, XiangYa Hosp 3, Ctr Expt Med, Changsha 410013, Hunan, Peoples R China.;[Yan, Yichao] Peking Univ Int Hosp, Dept Gastroenterol Surg, Beijing 102206, Peoples R China.;[Lin, Changwei] Cent South Univ, Sch Life Sci, Changsha 410078, Hunan, Peoples R China.

通讯机构： [Li, Xiaorong; Lin, Changwei] D;Department of Gastrointestinal surgery, The Third XiangYa Hospital of Central South University, Changsha, Hunan, 410013, China.;School of Life Sciences, Central South University, Changsha, Hunan, 410078, China.

摘要： The long noncoding RNA (lncRNA) LUCAT1 was recently reported to be upregulated and to play an essential role in multiple cancer types, especially colorectal cancer (CRC), but the molecular mechanisms of LUCAT1 in CRC are mostly unreported. Here, a systematic analysis of LUACT1 expression is performed with data from TCGA database and clinic CRC samples. LUCAT1 is identified as a putative oncogene, which is significantly upregulated in CRC and is associated with poor prognosis. Loss of LUCAT1 restricts CRC proliferative capacities in vitro and in vivo. Mechanically, NCL is identified as the protein binding partner of LUCAT1 by using chromatin isolation by RNA purification coupled with mass spectrometry (ChIRP-MS) and RNA immunoprecipitation assays. We also show that NCL directly binds to LUCAT1 via its putative G-quadruplex-forming regions from nucleotides 717 to 746. The interaction between LUCAT1 and NCL interferes NCL-mediated inhibition of MYC and promote the expression of MYC. Cells lacking LUCAT1 show a decreased MYC expression, and NCL knockdown rescue LUCAT1 depletion-induced inhibition of CRC cell proliferation and MYC expression. Our results suggest that LUCAT1 plays a critical role in CRC cell proliferation by inhibiting the function of NCL via its G-quadruplex structure and may serve as a new prognostic biomarker and effective therapeutic target for CRC. © 2020, The Author(s).

语种： 英文

作者： Zhang, Yunqiang*;Tu, Lijun;Zhou, Xiuhong;Li, Bin

期刊： Journal of BUON,2020年25(1):491-496 ISSN：1107-0625

通讯作者： Zhang, Yunqiang

作者机构： [Li, Bin; Zhang, Yunqiang] Chenzhou Peoples Hosp, Dept Neurosurg, Chenzhou 423000, Hunan, Peoples R China.;[Tu, Lijun] Chenzhou Peoples Hosp, Electrocardiog Room, Chenzhou 423000, Hunan, Peoples R China.;[Zhou, Xiuhong] Univ South China, Dept Pathophysiol, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Zhang, Yunqiang] C;Chenzhou Peoples Hosp, Dept Neurosurg, Chenzhou 423000, Hunan, Peoples R China.

关键词： glioma;microRNA;cell cycle arrest;SNAIL1;migration;invasion

摘要： Purpose: Gliomas are aggressive brain tumors accounting for significant mortality across the globe. Biomarkers for early detection and therapeutic targets for efficient treatment are lacking for glioma. This study was undertaken to investigate the role and therapeutic implications of miR-22 in glioma. Methods: U-87 glioma cell line was used in this study. qRT-PCR was employed for expression analysis. MTT assay was used for determination of cell viability. Lipofectamine 2000 was used for transfection. Flow cytometry was used for cell analysis. Wound healing assay and transwell assay were used for monitoring cell migration and invasion. Western blot analysis was used for estimation of protein expression. Results: The miR-22 expression was found decreased in glioma cells. Overexpression of miR-22 resulted in arrest of the U-87 glioma cells at G2/M checkpoint of the cell cycle. The percentage of apoptotic U-87 cells in G2/M phase were 13.05% in negative control (NC) and 29.06% in miR-22 mimics transfected cells. The cell cycle arrest promoted by miR-22 overexpression was also associated with depletion of cyclin B1 expression in U-87 cells. Furthermore, miR-22 could also significantly increase the sensitivity of glioma U-87 cells to cisplatin. The TargetScan analysis and dual luciferase assay showed SNAIL1 to be the target of miR-22. The expression of SNAIL1 was also enhanced in all the glioma cells and miR-22 overexpression could cause suppression of the SNAIL1 expression in U-87 cells. Furthermore, SNAIL1 silencing could also cause decline in the viability of the U-87 cells. The wound healing assay showed that miR-5 overexpression caused decrease in the migration of U-87 cells, while the transwell assay showed decline in the invasion of miR-22 mimics transfected U-87 cells. Conclusion: Taken together, miR-22 may exhibit therapeutic implications in glioma and may prove useful in glioma treatment. © 2020 Zerbinis Publications. All rights reserved.

语种： 英文

作者： Zhang, Yunsheng;Li, Fang;Liu, Luogen;Jiang, Hongtao;Hu, Hua;...

期刊： BMC Cancer,2019年19(1):1-13 ISSN：1471-2407

通讯作者： Wang, Yi

作者机构： [Wang, Yi; Zhang, Yunsheng; Ge, Xin; Liu, Luogen] Univ South China, Clin Res Inst, Affiliated Hosp 2, Hengyang 421001, Peoples R China.;[Zhang, Yunsheng] Clin Res Ctr Breast & Thyroid Dis Prevent Hunan P, Hengyang 421001, Peoples R China.;[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.;[Jiang, Hongtao] Univ South China, Dept Urol, Hosp 2, Hengyang 421001, Peoples R China.;[Hu, Hua] Univ South China, Canc Res Inst, Hosp 2, Hengyang 421001, Peoples R China.

通讯机构： [Wang, Yi] U;[Wang, Yi] H;Univ South China, Clin Res Inst, Affiliated Hosp 2, Hengyang 421001, Peoples R China.;Hainan Med Univ, Dept Urol, Affiliated Hosp 2, Haikou 570102, Hainan, Peoples R China.

关键词： Salinomycin;ATP2A3;ER stress;Ca2+ release;Apoptosis

摘要： Background: Salinomycin is a monocarboxylic polyether antibiotic and is a potential chemotherapy drug. Our previous studies showed that salinomycin inhibited cell growth and targeted CSCs in prostate cancer. However, the precise target of salinomycin action is unclear. Methods: In this work, we analyzed and identified differentially expressed genes (DEGs) after treatment with or without salinomycin using a gene expression microarray in vitro (PC-3 cells) and in vivo (NOD/SCID mice xenograft model generated from implanted PC-3 cells). Western blotting and immunohistochemical staining were used to analyze the expression of ATP2A3 and endoplasmic reticulum (ER) stress biomarkers. Flow cytometry was used to analyze the cell cycle, apoptosis and intracellular Ca2+ concentration. Results: A significantly upregulated gene, ATPase sarcoplasmatic/endoplasmatic reticulum Ca2+ transporting 3 (ATP2A3), was successfully identified. In subsequent studies, we found that ATP2A3 overexpression could trigger ER stress and exert anti-cancer effects in PC-3 and DU145 cells. ATP2A3 was slightly expressed, but the ER stress biomarkers showed strong staining in prostate cancer tissues. We also found that salinomycin could trigger ER stress, which might be related to ATP2A3-mediated Ca2+ release in PC-3 cells. Furthermore, we found that salinomycin-triggered ER stress could promote apoptosis and thus exert anti-cancer effects in prostate cancer cells. Conclusion: This study demonstrates that ATP2A3 might be one of the potential targets for salinomycin, which can inhibit Ca2+ release and trigger ER stress to exert anti-cancer effects. © 2019 The Author(s).

语种： 英文

作者： Tang, Yunlian;Zhong, Yating;Fu, Ting;Zhang, Yang;Cheng, Allan;...

期刊： Biomedicine & Pharmacotherapy,2019年116:108984 ISSN：0753-3322

通讯作者： Gan, Runliang

作者机构： [Gan, Runliang; Zhang, Yang; Dai, Yongming; Cheng, Allan; Qu, Jiani; Zhong, Yating; Tang, Yunlian; Fu, Ting] Univ South China, Med Coll Hengyang, Canc Res Inst, Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Peoples R China.;[Zhong, Yating] First Peoples Hosp Changde City, Dept Pathol, Changde 415000, Hunan, Peoples R China.

通讯机构： [Gan, Runliang] U;Univ South China, Med Coll Hengyang, Canc Res Inst, Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Peoples R China.

关键词： EB virus (EBV);Lymphocyte transformation;Lymphoblast;Gene expression profile;Key gene

摘要： Although the Epstein-Barr virus (EBV) is a well-known human oncogenic virus, its molecular mechanisms involved in the transformation of healthy human cells remain poorly understood. In this study, human lymphocytes were isolated from the peripheral blood of healthy adults, and lymphocytes were transformed in vitro by EBV. Agilent human whole genome microarrays were used to detect the differential gene expression profiles of EBV-transformed lymphoblasts and healthy peripheral blood lymphocytes (PBLs). By constructing the gene functional network of EBV-induced lymphocyte transformation, we screened out candidate key genes in this process and verified their expression levels by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. In the EBV-transformed lymphoblasts, 2335 differentially expressed genes, including 1328 up-regulated and 1007 down-regulated, were screened out. Five candidate key genes, namely, PLK1, E2F1, PTPN11, BIRC5 and FYN were mainly screened out according to the results of LIMMA, String, Cytoscape software analysis. RT-qPCR and Western blot showed that PLK1, E2F1, PTPN11, BIRC5 genes had increased expression levels, and FYN gene was down-regulated in EBV-transformed lymphoblasts. Silencing of PLK1 gene in Raji cells could inhibit cell proliferation and invasion, and induce cell cycle arrest and apoptosis. In conclusion, PLK1, E2F1, PTPN11, BIRC5 and FYN are the candidate key molecules of EBV-transformed lymphocytes. © 2019

语种： 英文

作者： Yajun Chenx;Xianpeng Dai;Yao Yao;Jing Wang;Xinzhi Yang;...

期刊： 生物化学与生物物理学报,2019年51(3):335-337 ISSN：1672-9145

通讯作者： Zhong, J.

作者机构： [Yao Yao; Zhong J.; Yang X.; Wang J.] Institute of Clinical Medicine, First Affiliated Hospital of University of South China, Hengyang, 421001, China;Department of Metabolism and Endocrinology, Second Affiliated Hospital of University of South China, Hengyang, 421001, China;[Dai X.] Department of General Surgery, Second Affiliated Hospital of University of South China, Hengyang, 421001, China;[Zhang Y.] Institute of Clinical Medicine, Second Affiliated Hospital of University of South China, Hengyang, 421001, China;Department of Metabolism and Endocrinology, First Affiliated Hospital of University of South China, Hengyang, 421001, China

通讯机构： [Zhong, J.] I;Institute of Clinical Medicine, First Affiliated Hospital of University of South China, Hengyang, China

关键词： MCF-7;apoptosis;expression;treatment;CYCLIN;can;Akt;its

摘要： Autophagy as a novel therapeutic target can inhibit or increase treatment efficacy in various types of breast cancer in a cell-type-dependent manner [1,2].Several studies have revealed that the coordination between Akt and the glycolytic pathway plays an indispensable role in mediating autophagy and caspase-dependent apoptosis,suggesting that a new regulatory mechanism for the process [3,4].Protein arginine N-methyltransferases(PRMTs)are eukaryotic enzymes that catalyze the transfer of methyl groups from S-adenosylmethionine to arginine residues of numerous PRMT substrates [5,6].PRMT2(also known as HRMT1L1)belongs to the arginine methyltransferase family [7].PRMT2β is a novel PRMT2 splice variant isolated from breast cancer cell [8].It occurs at the 3′ end of the PRMT2,resulting in loss of exons 7–9 and downstream frame-shifting [9].PRMT2β possesses 83 new amino acids at the C-terminus and its size is 301 amino acids.Our previous study reported that PRMT2β has potential antitumor effect by suppressing cyclin D1 expression [10].However,little is known about whether PRMT2β could regulate autophagy and glycolysis of MCF-7 cells.

语种： 英文

作者： Tan, Yuan;Li, Yumeng;Zhang, Yang;Yu, Jian;Wen, Yating;...

期刊： Immunologic Research,2018年66(4):471-479 ISSN：0257-277X

通讯作者： Wu, Yimou

作者机构： [Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.;[Tan, Yuan] First Hosp Changsha City, Dermatol, Changsha 410000, Hunan, Peoples R China.;[Zhang, Yang] Univ South China, Dept Pathol, Hengyang 421001, Peoples R China.

通讯机构： [Wu, Yimou] U;Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

关键词： *C. psittaci;*CD4+ T cells;*CPSIT_p7;*Plasmid protein;*Protective immunity

摘要： The present study evaluated the immune-protective efficacy of the Chlamydia psittaci (C. psittaci) plasmid protein CPSIT_p7 and analyzed the potential mechanisms of this protection. The current study used recombinant CPSIT_p7 protein with Freund’s complete adjuvant and Freund’s incomplete adjuvant to vaccinate BALB/c mice. Adjuvants alone or PBS formulated with the same adjuvants was used as negative controls. Mice were intranasally challenged with 105 inclusion-forming units (IFU) of C. psittaci. We found that CPSIT_p7 vaccination significantly decreased the mouse lung chlamydial load, interferon-γ (IFN-γ) level, and pathological injury. This protection correlated well with specific humoral and cellular immune responses against C. psittaci. In vitro or in vivo neutralization of C. psittaci with sera harvested from immunized mice did not reduce the number of recoverable C. psittaci in the infected lungs, but CD4+ spleen cells collected from CPSIT_p7-immunized mice significantly decreased the chlamydial load via adoptive transfer to native mice. These results reveal that the protection conferred by CPSIT_p7 is dependent on CD4+ T cells. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

语种： 英文

作者： Ding, Chengming;He, Jun;Zhao, Jun;Li, Junhua;Chen, Jie;...

期刊： Cell Proliferation,2018年51(5):e12464- ISSN：0960-7722

通讯作者： Peng, Jian;Xia, Zanxian

作者机构： [Jia, Zeming; Chen, Jie; Ding, Chengming; Zhang, Yaoting; Peng, Jian] Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha, Hunan, Peoples R China.;[He, Jun; Li, Chong; Ding, Chengming; Zhu, Zhu; Han, Dong] Univ South China, Dept Hepatopancreatobiliary Surg, Affiliated Hosp 1, Hengyang, Peoples R China.;[Li, Junhua; Ding, Chengming; Zhao, Jun] Univ Southern Calif, Dept Mol Microbiol & Immunol, Norris Comprehens Canc Ctr, Los Angeles, CA USA.;[Liao, Wenyan] Univ South China, Dept Obstet & Gynecol, Affiliated Hosp 1, Hengyang, Peoples R China.;[Zeng, Yi] Youjiang Med Univ Nationalities, Dept Pathol & Immunol, Baise, Peoples R China.

通讯机构： [Peng, Jian; Xia, Zanxian] C;Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha, Hunan, Peoples R China.;Cent S Univ, State Key Lab Med Genet, Changsha, Hunan, Peoples R China.;Cent S Univ, Sch Life Sci, Changsha, Hunan, Peoples R China.

关键词： colorectal cancer;innate immunity;IRF3;RIG-I-like receptor (RLR)-mediated IFN-β signalling pathway;β-catenin

摘要： Objective: β-catenin is one of the most critical oncogenes associated with many kinds of human cancers, especially in the human CRC. Innate immunity recognizes tumour derived damage-associated molecular patterns (DAMPs) and primes the anti-tumour adaptive responses. While the function of β-catenin in CRC tumourigenesis is well established, its impact on innate immune evasion is largely unknown. The aim of this study is to characterize the role of β-catenin in inhibiting RIG-I-like receptor (RLR)-mediated IFN-β signalling in colorectal cancer. Materials and Methods: Immunohistochemical staining and western blotting were conducted to study the expression of β-catenin, IRF3 and phospho-IRF3 (p-IRF3) in CRC samples and cell lines. Plaque assay determining virus replication was performed to assess the regulation of β-catenin on IFN-β signalling. The inhibition of β-catenin on RLR-mediated IFN-β signalling was further studied by real-time analyses and reporter assays in the context of lentiviral-mediated β-catenin stably knocking down. Lastly, co-immunoprecipitation and nuclear fractionation assay were conducted to monitor the interaction between β-catenin and IRF3. Results: We found that high expression of β-catenin positively correlated with the expression of IRF3 in CRC cells. Overexpression of β-catenin increased the viral replication. Conversely knocking down of β-catenin inhibited viral replication. Furthermore, our data demonstrated that β-catenin could inhibit the expression of IFN-β and interferon-stimulated gene 56 (ISG56). Mechanistically, we found that β-catenin interacted with IRF3 and blocked its nuclear translocation. Conclusion: Our study reveals an unprecedented role of β-catenin in enabling innate immune evasion in CRC. © 2018 John Wiley & Sons Ltd

语种： 英文

作者： Zhang, Yunqiang;Tu, Lijun;Zhou, Xiuhong;Li, Bin

期刊： Medical science monitor basic research,2018年24:216-224 ISSN：2325-4394

作者机构： [Li, Bin; Zhang, Yunqiang] Department of Neurosurgery, Chenzhou No. 1 People's Hospital, Chenzhou, Hunan, China (mainland);[Tu, Lijun] Electrocardiographic Room, Chenzhou No. 1 People's Hospital, Chenzhou, Hunan, China (mainland);[Zhou, Xiuhong] Department of Pathophysiology, University of South China, Hengyang, Hunan, China (mainland)

摘要： BACKGROUND Curcumin has clear anti-tumor activity in various carcinomas. It regulates various signaling pathways like Wnt/β-catenin and JAK2/STAT3, which play vital roles in cell proliferation of several carcinomas, but to the best of our knowledge, there are currently no published reports on human glioma CHME cells. Therefore, the aim of this study was to explore the effect of curcumin on human glioma CHME cells. MATERIAL AND METHODS The CHME cell line was purchased from American Type Culture Collection (ATCC). The expressions of caspases 3, caspases 9, PARP, BAX, and BCL2 were detected by Western blot. Annexin V FITC, mitochondrial membrane potential, and reactive oxygen species were detected by flow cytometry. DAPI staining was detected by fluorescence microscopy. Cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. RESULTS We found that curcumin has cytotoxic activity in human glioma CHME cells, as shown by DAPI staining, annexin V/PI, and nuclear morphology. We found that cell growth decreased with increased concentration of curcumin, as well as sowing effects on expression of caspase-3, caspase-9, and cleavage of PARP, which suggests apoptotic cascade activity. The increase in reactive oxygen species and loss of mitochondrial membrane potential (Δψmt) in concentration-dependent manners suggests biochemical induction of apoptosis in CHME cells. CONCLUSIONS Curcumin has effective anticancer activity in human glioma CHME cells by inducing the apoptotic pathway.

语种： 英文

作者： 王文松;黄英;李清叶;刘芳;张杨;...

期刊： 中南医学科学杂志,2018年46(2):119-122+135 ISSN：2095-1116

作者机构： 南华大学医学院肿瘤研究所,湖南衡阳,421001;[黄英] 常德职业技术学院;湖南环境生物学院医学院;[王文松; 刘芳; 肖国华; 谭晖; 张杨] 南华大学;[李清叶] 湖南环境生物职业技术学院

关键词： 非霍奇金淋巴瘤;组织芯片;DJ-1蛋白表达

摘要： DJ-1是PARK7基因编码的一种蛋白质,其过表达与许多恶性肿瘤的发生、侵袭、转移及预后密切相关。然而,有关DJ-1蛋白在非霍奇金淋巴瘤（NHL）中的表达与NHL生物学特性的关系尚未见文献报导。本文检测了61例NHL组织中DJ-1蛋白在细胞核内外的表达情况,发现DJ-1蛋白在细胞浆内高表达的情况较细胞核内更为显著（93.4%、72.1%）,且都显著高于非肿瘤组织（28.6%、14.3%）。此外,DJ-1蛋白的高表达率与NHL的恶性程度正相关,DJ-1蛋白在惰性、侵袭性和高侵袭性NHL中核高表达率为52.2%、82.9%和100%;浆高表达率为82.6%、100%和100%。结果提示DJ-1蛋白在NHL细胞内的定位可能是一个动态过程,而这与肿瘤的发生和发展密切相关;DJ-1与NHL的转移和侵袭等恶性进程密切相关,DJ-1是极具潜力的NHL相关的肿瘤生物学标记物。

语种： 中文

作者： Zhang, Yunsheng;Li, Fang;Liu, Luogen;Jiang, Hongtao;Jiang, Xiaorong;...

期刊： Life Sciences,2018年207:451-460 ISSN：0024-3205

通讯作者： Wang, Yi

作者机构： [Wang, Yi; Zhang, Yunsheng; Ge, Xin; Liu, Luogen] Univ South China, Affiliated Hosp 2, Clin Res Inst, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.;[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.;[Jiang, Hongtao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang 421001, Peoples R China.;[Wang, Zhenggen; Zhang, Li; Jiang, Xiaorong] Univ South China, Affiliated Hosp 2, Dept Gastroenterol, Hengyang 421001, Peoples R China.;[Cao, Jingsong] Univ South China, Med Coll, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

通讯机构： [Wang, Yi] U;Univ South China, Affiliated Hosp 2, Clin Res Inst, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.

关键词： ATG3/AKT/mTOR signaling axis;Autophagy;PC-3 cells;Salinomycin

摘要： Aims: This study evaluated the mechanism by which salinomycin-induced autophagy blocks apoptosis in PC-3 prostate cancer cells. Main methods: The anti-cancer effects of salinomycin in PC-3 cells were confirmed by flow cytometry, JC-1 staining and western blotting. Then, the autophagic effects were measured by western blotting, GFP-LC3 puncta formation assay, immunofluorescence staining and electron microscopy. Furthermore, we used lentivirus-mediated shRNA to silence ATG3, ATG5 and ATG7 expression in PC-3 cells to investigate the regulatory mechanisms of salinomycin-induced autophagy. Key findings: Salinomycin could induce apoptosis and autophagy in PC-3 cells. Interestingly, autophagy inhibition could enhance salinomycin-induced apoptosis. We further showed that ATG3, a known critical regulator of autophagy, was downregulated and involved in the inhibition of apoptosis by salinomycin-induced autophagy via the AKT/mTOR signaling axis. Significance: Our data indicated that salinomycin-induced autophagy blocks apoptosis via the ATG3/AKT/mTOR signaling axis in PC-3 cells, which provides new clues for the mechanisms of underlying the anti-cancer effects of salinomycin. © 2018

语种： 英文

作者： 张杨;唐运莲;王成昆

期刊： 课程教育研究,2017年(27):142-143 ISSN：2095-3089

作者机构： 南华大学医学院病理学教研室

关键词： 病理学教学;思维导图;教学效果

摘要： 目的:分析思维导图结合PPT在病理学教学中的教学效果。方法:将我校13级本科临床医学专业的76名学生作为此次观察分析的对象,按随机分配的方法将其分为对照组与研究组,对照组的总例数为38例,给予该组学生PPT理论教学的方法,研究组的总例数为38例,给予该组学生思维导图结合PPT理论教学的方法,比较并分析两组学生的成绩。结果:对照组的成绩比研究组低,组间数据进行比较,有统计学差异存在（P<0.05）。结论:将思维导图结合PPT应用于病理学的教学中,可有效提升教学的质量,建议将该方法应用与推广。

语种： 中文

作者： Cao, Jingsong;Liu, Luogen;Zhang, Yunsheng;Xiao, Jianhua*;Wang, Yi*

期刊： BMC Immunology,2017年18(1):1-8 ISSN：1471-2172

通讯作者： Xiao, Jianhua;Wang, Yi

作者机构： [Xiao, Jianhua; Cao, Jingsong] Univ South China, Med Coll, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Jianhua; Cao, Jingsong] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Wang, Yi; Zhang, Yunsheng; Xiao, JH; Wang, Y; Xiao, Jianhua; Liu, Luogen; Cao, Jingsong] Univ South China, Affiliated Hosp 2, Inst Pathogen Biol, Clin Res Ctr,Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Wang, Yi] Univ South China, Affiliated Hosp 2, Urinary Surg, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Xiao, Jianhua; Xiao, JH; Wang, Y] U;Univ South China, Med Coll, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 2, Inst Pathogen Biol, Clin Res Ctr,Med Coll, Hengyang 421001, Hunan, Peoples R China.

关键词： Blood group B antigen;Blood group B antibody;HK2;B cells activation;ABOi-KT

摘要： Background: It is well known that ABO blood group system incompatible kidney transplantation (ABOi-KT) is an effective strategy for end-stage renal disease. The main barrier for ABOi-KT is how to keep host B cell activation and blood group antibody titer in low levels. Moreover, the mechanism of B cell activation induced by blood group antigen was unclear in ABOi-KT. Results: In this study, HK2 cells were identified to express blood group B antigen when cocultured with lymphocytes of blood group A. Optical microscope observation demonstrated that HK2 cells in coculture group gradually decreased. Furthermore, flow cytometer assay identified that T cell phenotypes (CD3+, CD3+CD4+ and CD3+CD8+) had no significant change and B cell phenotypes (CD19+ and CD138+) were all significantly enhanced (3.07 and 3.02 folds) at day 4. In addition, immunoturbidimetry analysis demonstrated that blood group B antibody was significantly increased to 2.35 fold at day 4, IgG was significantly increased to 3.60 and 2.81 folds at days 4 and 8 respectively, while IgM had no significant change at the measured time points. Conclusions: Taken together, B cells were activated and secreted blood group B antibody after treatment with HK2 expressing blood group B antigen. The results of this study maybe useful for further determination of the mechanism of B cell activation after ABO incompatible kidney endothelial cells stimulation. © 2017 The Author(s).

语种： 英文

作者： 张杨;彭波;唐运莲;程爱兰

期刊： 课程教育研究,2017年(24):116-117 ISSN：2095-3089

作者机构： 南华大学医学院病理学教研室

关键词： 微课;病理学;学习效果;上课积极性

摘要： 目的:探究微课早病理学教学中的应用效果。方法:选取我院2014级临床医学专业的学生作为研究的对象,将其分为对照组和观察组两组。对照组学生给予常规病理学教学,观察组学生则给予微课病理学教学,比较两组学生的病理学学习效果及上课积极性。结果:观察组学生的病理学理论成绩82.74±5.06分、操作成绩84.56±5.37分均高于对照组学生的74.83±4.48分、75.69±4.77分,上课积极率97.14%也高于对照组学生74.29%,均有统计学意义(P<0.05)。结论:微课在病理学教学实践中能够提高学生的学习积极性与学习效果,值得推广。

语种： 中文

作者： Deng, Hongli;Li, Zhibo;Liu, Guang;Li, Xianhua;Chen, Yong;...

期刊： Gynecological Endocrinology,2017年33(5):363-367 ISSN：0951-3590

通讯作者： Zhang, Yong;Sun, Yifan;Fu, Jinjian

作者机构： [Deng, Hongli; Liu, Guang; Li, Zhibo; Li, Xianhua] Univ South China, Peoples Hosp Liuyang City, Dept Clin Lab, Affiliated Liuyang Hosp, Changsha, Hunan, Peoples R China.;[Chen, Yong] Univ South China, Dept Clin Lab, Affiliated Changsha Hosp, Hosp Changsha City 1, Changsha, Hunan, Peoples R China.;[Zhang, Yong] Univ South China, Dept Gastrointestinal Surg, Affiliated Liuyang Hosp, Peoples Hosp Liuyang City, Renmin Rd 119, Changsha 410300, Hunan, Peoples R China.;[Sun, Yifan] Guangxi Univ Chinese Med, Dept Clin Lab, Affiliated Hosp 3, 32 Jiefang North Rd, Liuzhou 545001, Guangxi, Peoples R China.;[Fu, Jinjian] Liuzhou Matern & Child Hlth Care Hosp, Dept Clin Lab, 50th Yingshan Rd, Liuzhou 545001, Guangxi, Peoples R China.

通讯机构： [Zhang, Yong] U;[Sun, Yifan] G;[Fu, Jinjian] L;Univ South China, Dept Gastrointestinal Surg, Affiliated Liuyang Hosp, Peoples Hosp Liuyang City, Renmin Rd 119, Changsha 410300, Hunan, Peoples R China.;Guangxi Univ Chinese Med, Dept Clin Lab, Affiliated Hosp 3, 32 Jiefang North Rd, Liuzhou 545001, Guangxi, Peoples R China.

关键词： Biomarker;inflammatory;insulin resistance;interferon gamma-induced protein 10;polycystic ovary syndrome

摘要： Background: Interferon γ-induced protein 10 kDa (IP10/CXCL10) is a chemokine related to endocrine disorders; however, the serum concentrations of IP10 in women with polycystic ovary syndrome (PCOS) have not yet been reported. Therefore, we investigated whether IP10 is increased in PCOS patients and its potential clinical value in PCOS patients. Methods: For this research, the serum IP10, glucose, insulin, high sensitivity C-reactive protein (hs-CRP), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and total testosterone (TT) concentrations were measured in 60 women with PCOS and healthy controls. Results: The median IP10 concentration was 45.60 pg/mL [interquartile range (IQR):29.75, 79.69], which was significantly higher than that of the body mass index (BMI)-matched controls (median: 36.46 pg/mL; IQR:28.98, 45.80). In the multivariate linear regression analysis, hs-CRP and the homeostasis model assessment of insulin resistance index (HOMA2-IR) were independent predictors of the IP10 values, while FSH was inversely associated with the IP10.No significant association was observed between the IP10 and BMI, glucose, LH and TT. Conclusions: The serum IP10 concentrations increase in women with PCOS, moreover, IP10 appears to be correlated with the inflammatory and IR statuses of PCOS. IP10 may be a potential biomarker to estimate the disease activity of PCOS. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： Zhang, Yunsheng;Liu, Luogen;Li, Fang;Wu, Tao;Jiang, Hongtao;...

期刊： BioMed Research International,2017年2017:4101653 ISSN：2314-6133

通讯作者： Wang, Yi

作者机构： [Wang, Yi; Zhang, Yunsheng; Liu, Luogen] Univ South China, Affiliated Hosp 2, Clin Res Inst, Hengyang 421001, Peoples R China.;[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.;[Wang, Yi; Wu, Tao; Jiang, Xianxun; Jiang, Hongtao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang 421001, Peoples R China.;[Du, Xiaobo] First Peoples Hosp Yueyang, Dept Urol, Yueyang 414000, Peoples R China.

通讯机构： [Wang, Yi] U;Univ South China, Affiliated Hosp 2, Clin Res Inst, Hengyang 421001, Peoples R China.

摘要： Salinomycin is an antibiotic isolated from Streptomyces albus that selectively kills cancer stem cells (CSCs). However, the antitumor mechanism of salinomycin is unclear. This study investigated the chemotherapeutic efficacy of salinomycin in human prostate cancer PC-3 cells. We found that cytotoxicity of salinomycin to PC-3 cells was stronger than to nonmalignant prostate cell RWPE-1, and exposure to salinomycin induced G2/M phage arrest and apoptosis of PC-3 cells. A mechanistic study found salinomycin suppressed Wnt/β-catenin pathway to induce apoptosis of PC-3 cells. An in vivo experiment confirmed that salinomycin suppressed tumorigenesis in a NOD/SCID mice xenograft model generated from implanted PC-3 cells by inhibiting the Wnt/β-catenin pathway, since the total β-catenin protein level was reduced and the downstream target c-Myc level was significantly downregulated. We also showed that salinomycin, but not paclitaxel, triggered more apoptosis in aldehyde dehydrogenase- (ALDH-) positive PC-3 cells, which were considered as the prostate cancer stem cells, suggesting that salinomycin may be a promising chemotherapeutic to target CSCs. In conclusion, this study suggests that salinomycin reduces resistance and relapse of prostate tumor by killing cancer cells as well as CSCs. © 2017 Yunsheng Zhang et al.

语种： 英文

作者： Chen, Guodong;Tang, Na;Wang, Chao;Xiao, Linqiao;Yu, Minjun;...

期刊： Biochemical and Biophysical Research Communications,2017年484(2):311-317 ISSN：0006-291X

通讯作者： Zhang, Yan

作者机构： [Han, Liang; Wang, Chao; Chen, Guodong; Tang, Na; Zhao, Lanhua; Cai, Hengling; Xie, Chengyuan; Yu, Minjun; Zhang, Yan; Xiao, Linqiao] Univ South China, Coll Med, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Zhang, Yan] U;Univ South China, Coll Med, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： Helicobacter pylori;TNF-alpha inducing protein (Tip alpha);IL-6/STAT3 pathway;Gastric cancer;Epithelial-mesenchymal transition (EMT)

摘要： Tumor necrosis factor (TNF)-α-inducing protein (Tipα) is a newly identified carcinogenic factor secreted by Helicobacter pylori (H. pylori). Although it has been proved that Tipα is a strong inducer of epithelial-mesenchymal transition (EMT), a crucial process of migration, the exact molecular mechanism is unknown. Current evidence indicates that the oncogenic transcription factor signal transducers and activators of transcription 3 (STAT3) is inappropriately activated in multiple malignancies, including gastric cancer. In this study, we showed that Tipα significantly down-regulated the expression of EMT-related markers E-cadherin as well as up-regulated N-cadherin and vimentin in SGC7901 cells, with typical morphological changes of EMT. Tipα also promoted proliferation and migration of SGC7901 cells. Furthermore, Tipα activated interleukin-6 (IL-6)/STAT3 signaling pathway in SGC7901 cells. The effects of Tipα treatment observed was abolished when we block IL-6/STAT3 signaling pathway. Altogether, our data demonstrated that Tipα may accelerate tumor aggressiveness in gastric cancer by promoting EMT through activation of IL-6/STAT3 pathway. © 2017 Elsevier Inc.

语种： 英文

作者： 尹华丽;张杨;甘润良

期刊： 中华病理学杂志,2017年46(9):659-661 ISSN：0529-5807

作者机构： [尹华丽; 张杨; 甘润良] 南华大学医学院肿瘤研究所, 湖南, 衡阳, 421001

关键词： EB病毒;细胞;肿瘤

摘要： 肿瘤是一类细胞周期疾病。人体内存在精密的细胞周期调控机制,细胞周期不正确地运行,使细胞获得以增殖过多、凋亡过少为主要形式的失控性生长特征,导致肿瘤发生。在人类细胞周期的G_1晚期,存在一个“限制点”(R点),决定细胞是否进入S期。相应生长因子或增殖信号的存在,使得细胞从G。/G,期,通过R点进入S期,完成整个细胞分裂周期。

语种： 中文

作者： Li, Bi-Rong;Xia, Lin-Qin;Liu, Jing;Liao, Lin-Ling;Zhang, Yang;...

期刊： Biochemical and Biophysical Research Communications,2017年494(1-2):384-389 ISSN：0006-291X

通讯作者： He, Ping-Ping;Ouyang, Xin-Ping

作者机构： [Zhang, Yang; Deng, Min; Li, Bi-Rong] Shaoyang Med Coll, Dept Physiol, Shaoyang 422000, Hunan, Peoples R China.;[He, Ping-Ping; Ouyang, Xin-Ping] Univ South China, Med Coll, Neurosci Inst, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;[Xia, Lin-Qin] Shaoyang Med Coll, Dept Pathol, Shaoyang 422000, Hunan, Peoples R China.;[Liu, Jing] Shaoyang Med Coll, Fac Nursing, Shaoyang 422000, Hunan, Peoples R China.;[Liao, Lin-Ling] Shaoyang Med Coll, Affiliated Hosp, Dept Hematol Oncol, Shaoyang 422000, Hunan, Peoples R China.

通讯机构： [He, Ping-Ping; Ouyang, Xin-Ping] U;Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Med Coll, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.

关键词： *Atherosclerosis;*CD36;*Cholesterol uptake;*miR-758-5p

摘要： miR-758-3p plays an important role via regulting ABCA1-mediated cholesterol efflux in atherosclerosis. However, the mechanism of miR-758-5p in cholesterol metabolism is still unclear. Here, we revealed that miR-758-5p decreased total cholesterol accumulation in THP-1 macrophage derived foam cells through markedly reducing cholesterol uptake, and no effect on the cholesterol efflux. Interestingly, computational analysis suggests that CD36 may be a target gene of miR-758-5p. Our study further demonstrated that miR-758-5p decreased CD36 expression at both protein and mRNA levels via targeting the CD36 3′UTR in THP-1 macrophage derived foam cells. The present present study concluded that miR-758-5p decreases lipid accumulation of foam cell via regulating CD36-mediated the cholesterol uptake. Therefore, targeting miR-758-5p may offer a promising strategy to treat atherosclerotic vascular disease. © 2017

语种： 英文

作者： 张杨;唐双阳;王成昆;曾燚华

期刊： 西部素质教育,2017年3(12):109 ISSN：2095-6401

作者机构： 南华大学医学院微生物学教研室,湖南 衡阳,421001

关键词： 实验教学;教学改革;病原微生物学

摘要： 医学生要全面掌握基础医学和临床医学知识就必须先掌握好病原微生物学的知识。文章首先论述了病原微生物学进行实验教学改革的重要性,其次分析了病原微生物学传统实验教学中存在的问题,最后提出了病原微生物学实验教学改革的策略。

语种： 中文

作者： 游咏;莫靓;许丽芳;尹凯;李熠;...

期刊： 教育教学论坛,2017年(35):193-194 ISSN：1674-9324

作者机构： 南华大学医学院,湖南 衡阳,421001;南华大学附属第一医院,湖南 衡阳,421001

会议名称： 第20届全国高等医学院校诊断学教学改革研讨会

会议时间： 2017-08-01

会议地点： 湖南衡阳

会议论文集名称： 第20届全国高等医学院校诊断学教学改革研讨会论文集

关键词： 临床技能学;双语;网络教学

摘要： 临床技能学是从临床技能实践教学的整体出发,以基本临床技能操作与综合临床技能为主要教学内容的实践性课程。及时了解、跟进临床技能学领域的最新理论、最新方法与技术是临床技能学教学中不可或缺的重要组成部分。临床技能学双语网络教学平台的构建对于培养全面发展创新型医学人才有着重要的意义。

语种： 中文