Salinomycin-induced autophagy blocks apoptosis via the ATG3/AKT/mTOR signaling axis in PC-3 cells

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhang, Yunsheng;Li, Fang;Liu, Luogen;Jiang, Hongtao;Jiang, Xiaorong;...

通讯作者：

Wang, Yi

作者机构：

[Wang, Yi; Zhang, Yunsheng; Ge, Xin; Liu, Luogen] Univ South China, Affiliated Hosp 2, Clin Res Inst, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.

[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.

[Jiang, Hongtao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang 421001, Peoples R China.

[Wang, Zhenggen; Zhang, Li; Jiang, Xiaorong] Univ South China, Affiliated Hosp 2, Dept Gastroenterol, Hengyang 421001, Peoples R China.

[Cao, Jingsong] Univ South China, Med Coll, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

通讯机构：

[Wang, Yi] U

Univ South China, Affiliated Hosp 2, Clin Res Inst, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.

语种：

英文

关键词：

autophagy related protein;autophagy related protein 5;autophagy related protein 7;mammalian target of rapamycin;protein ATG3;protein kinase B;salinomycin;short hairpin RNA;unclassified drug;AKT1 protein, human;antineoplastic agent;ATG3 protein, human;autophagy related protein;green fluorescent protein;MTOR protein, human;protein kinase B;pyran derivative;salinomycin;target of rapamycin kinase;ubiquitin conjugating enzyme;Akt/mTOR signaling;antineoplastic activity;apoptosis;Article;ATG3 gene;ATG5 gene;ATG7 gene;autophagy;controlled study;down regulation;drug mechanism;drug screening;electron microscopy;flow cytometry;gene expression;gene silencing;human;human cell;immunofluorescence;PC 3 cell line;prostate cancer cell line;Western blotting;cell proliferation;drug effect;male;metabolism;prostate tumor;signal transduction;tumor cell line;Antineoplastic Agents;Apoptosis;Autophagy;Autophagy-Related Proteins;Cell Line, Tumor;Cell Proliferation;Green Fluorescent Proteins;Humans;Male;Prostatic Neoplasms;Proto-Oncogene Proteins c-akt;Pyrans;Signal Transduction;TOR Serine-Threonine Kinases;Ubiquitin-Conjugating Enzymes

期刊：

Life Sciences

ISSN：

0024-3205

年：

2018

卷：

207

页码：

451-460

DOI：

10.1016/j.lfs.2018.06.034

基金类别：

This work was supported by the National Natural Science Foundation of China (nos. 81241090 and 81372378 ) and the Natural Science Foundation of Hunan Province (nos. 2018JJ3462 and 2016WK2013 ). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. We are grateful to Professor Zhiqiang Liu from the Department of Pathophysiology at Tianjin Medical University and Dan Li from the College of Biology at Hunan University for their helpful scientific discussions. This work was supported by the National Natural Science Foundation of China (nos. 81241090 and 81372378) and the Natural Science Foundation of Hunan Province (nos. 2018JJ3462 and 2016WK2013). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. We are grateful to Professor Zhiqiang Liu from the Department of Pathophysiology at Tianjin Medical University and Dan Li from the College of Biology at Hunan University for their helpful scientific discussions.

机构署名：

本校为第一且通讯机构

院系归属：

医学院

摘要：

Aims: This study evaluated the mechanism by which salinomycin-induced autophagy blocks apoptosis in PC-3 prostate cancer cells. Main methods: The anti-cancer effects of salinomycin in PC-3 cells were confirmed by flow cytometry, JC-1 staining and western blotting. Then, the autophagic effects were measured by western blotting, GFP-LC3 puncta formation assay, immunofluorescence staining and electron microscopy. Furthermore, we used lentivirus-mediated shRNA to silence ATG3, ATG5 and ATG7 expression in PC-3 cells to investigate the regulatory mec...

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Salinomycin-induced autophagy blocks apoptosis via the ATG3/AKT/mTOR signaling axis in PC-3 cells

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