期刊：
AMERICAN JOURNAL OF PATHOLOGY,2025年195(10):1766-1775 ISSN：0002-9440
通讯作者：
Chen, LL
作者机构：
[Hu, Canming; Liu, Zhe; Luo, Xiang; Bai, Qinqin; Wang, Xin; Chen, Lili] Univ South China, Sch Publ Hlth, Dept Publ Hlth Lab Sci, Hengyang, Peoples R China.;[Hu, Canming; Liu, Zhe; Luo, Xiang; Bai, Qinqin; Wang, Xin; Chen, Lili] Univ South China, Sch Basic Med, Dept Publ Hlth Lab Sci, Hengyang, Peoples R China.;[Lu, Chunxue; Chen, Shenghua] Univ South China, Hengyang Med Sch, Hengyang, Peoples R China.;[Lu, Chunxue; Chen, Shenghua] Univ South China, Dept Med Record, Hengyang, Peoples R China.;[Zeng, Xindian] Univ South China, Affiliated Nanhua Hosp, Hengyang, Peoples R China.
通讯机构：
[Chen, LL ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.
摘要：
Chlamydia species are responsible for significant diseases in both humans and animals, with their infection processes involving complex interactions with host cells. Protein post-translational modifications (PTMs) have emerged as a critical focus in understanding the complex interplay between Chlamydia and its host. PTMs, including phosphorylation, glycosylation, ubiquitination, acetylation, and methylation, play pivotal roles in regulating key processes during Chlamydia infection, such as bacterial invasion, intracellular survival, immune evasion, and manipulation of host signaling pathways. By modulating both bacterial and host proteins, PTMs serve as essential mechanisms that shape the progression and outcome of Chlamydia infections. This growing area of research not only deepens the understanding of Chlamydia 's pathogenic strategies but also opens new avenues for developing targeted therapeutic interventions against these infections.
Chlamydia species are responsible for significant diseases in both humans and animals, with their infection processes involving complex interactions with host cells. Protein post-translational modifications (PTMs) have emerged as a critical focus in understanding the complex interplay between Chlamydia and its host. PTMs, including phosphorylation, glycosylation, ubiquitination, acetylation, and methylation, play pivotal roles in regulating key processes during Chlamydia infection, such as bacterial invasion, intracellular survival, immune evasion, and manipulation of host signaling pathways. By modulating both bacterial and host proteins, PTMs serve as essential mechanisms that shape the progression and outcome of Chlamydia infections. This growing area of research not only deepens the understanding of Chlamydia 's pathogenic strategies but also opens new avenues for developing targeted therapeutic interventions against these infections.
摘要：
The association between antenatal antidepressant use and pregnancy-induced hypertension (GH/PE) remains controversial, with conflicting evidence on whether depression itself confounds this risk. We searched PubMed, Web of Science, Medline, Embase, Cochrane Library, and the China National Knowledge Infrastructure electronic databases from inception to Dec. 31, 2024. Eligibility criteria for selecting studies are randomized trials, cohort studies, and case-control studies exploring the association between antidepressant use during pregnancy and pregnancy-induced hypertension. Two reviewers extracted data and assessed quality. Outcomes were expressed as odds ratios with 95% confidence intervals. Seventeen observational studies were analyzed, indicating a potential link between antidepressant use in pregnancy and an elevated risk of gestational hypertension/preeclampsia (GH/PE). Pooled odds ratios (ORs) stratified by drug class demonstrated significant associations for: Selective serotonin reuptake inhibitors (OR = 1.34; 95% CI: 1.14–1.58); Serotonin-norepinephrine reuptake inhibitors (OR = 1.94; 95% CI: 1.40–2.70); Tricyclic antidepressants (OR = 2.00; 95% CI: 1.32–3.04). Following adjustment for depression as a confounding variable, sensitivity analysis indicated: SNRIs maintained significant association with GH/PE (adjusted OR = 1.53; 95% CI: 1.25–1.88); SSRIs showed no significant risk (adjusted OR = 1.03; 95% CI: 0.97–1.10); TCAs lost statistical significance (adjusted OR = 1.80; 95% CI: 0.86–3.74). Subgroup analyses suggesting geographical variations in effect magnitude. This study demonstrates differential associations between antenatal exposure to three antidepressant classes (SSRIs, SNRIs, TCAs) and gestational hypertension/preeclampsia risk. Enhanced blood pressure monitoring is advised during SNRI therapy, with clinical decisions requiring individualized risk-benefit assessment. Residual confounding constrains causal inference, necessitating prospective studies with standardized depression scales and systematic exposure documentation to resolve methodological limitations. PROSPERO: CRD42025640646.
作者：
Xudong Li;Yajie Wang;Tiancheng Zhang;Weiqing Rang;Yan Liu
期刊：
Journal of Diabetes and Metabolic Disorders,2025年24(2):1-14 ISSN：2251-6581
通讯作者：
Rang, Weiqing;Liu, Y
作者机构：
[Yan Liu; Weiqing Rang; Xudong Li; Yajie Wang; Tiancheng Zhang] School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan China. ROR: https://ror.org/03mqfn238. GRID: grid.412017.1. ISNI: 0000 0001 0266 8918
通讯机构：
[Weiqing Rang; Yan Liu] S;School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang, China
关键词：
Diabetes mellitus;Cold temperature;Hot temperature;Global burden of disease;Economic burden
摘要：
AIMS: This study investigated the impact of epidemiological and demographic Changes on the health and economic burdens of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) attributed to non-optimal temperature. METHODS: Mortality data were from the 2021 Global Burden of Disease (GBD) database, while economic data were sourced from multiple databases. The study analyzed global and regional trends in diabetes deaths, mortality rates, and years of life lost (YLL) due to non-optimal temperatures from 1990 to 2021, considering factors such as the Socio-demographic Index (SDI), gender, region, and age. The economic impact was assessed using YLL and labor market indicators in the 50 most populous countries. RESULTS: In 2021, 102,872 T2DM deaths globally were attributed to non-optimal temperatures, with low-temperature ASMR 1.61 times higher than high-temperature ASMR. From 1990 to 2021, Age-Standardized Mortality Rate(ASMR) for T2DM attributed to high and low temperatures increased significantly (84.17% and 24.06%) in low-to-middle SDI regions. Older adults had the highest mortality rate, and males faced higher risks than females. ASMR peaked at an SDI of 0.48 and decreased with increasing SDI. From 2022 to 2030, the female ASMR from T2DM attributed to non-optimal temperatures is projected to rise by 26.54% in Pakistan and 42.61% in Nepal. CONCLUSION: Low temperatures remain a major mortality driver, with elderly males and low-SDI populations most at risk. Targeting young males with preventive measures can reduce future mortality. Countries with large populations and low SDI should prioritize temperature interventions to address climate change. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40200-025-01717-2.
摘要：
Monitoring pesticide residues and lanthanide metals in water system is crucial for safeguarding human health and environmental integrity. Herein, we precisely designed a type of Mn-clay-based composite nanozyme via interfacial engineering and, for the first time, evaluated the effect of the interfacial configuration of glyphosate (Glyp) on laccase-like activity using in-situ ATR-FTIR spectroscopy and mass spectrometry . DFT calculations revealed that the phosphonate group interacts with Mn - Mn to form inner-sphere complexes with multiple configurations tailoring electron transfer and redox ability. Ce 3+ , serving as a hitherto unreported inhibitor of Glyp, can induce the self-coupling consumption of free Glyp, and irreversibly “turn off” latter interfacial laccase-amplification effect by changing its reactive adsorbed configuration. A similar phenomenon can be witnessed in other lanthanide metals (Ln 3+ ), e.g ., La 3+ , Pr 3+ , Nd 3+ , Sm 3+ , Eu 3+ , Gd 3+ , Tb 3+ , Dy 3+ and Er 3+ . With this in mind, an “on-off” enzyme-free nanosensor was designed based on laccase-mimicking cascade catalytic reaction for the selective recognition of Glyp and Ln 3+ . Our nanosensor demonstrates a good linear relationship with Glyp in a wide range of 0–0.2 mM (0–3 μM for Ce 3+ ), with a low LOD of 61 nM for Glyp and 94 nM for Ce 3+ . Additionally, it exhibits high selectivity and anti-interference ability under the co-application of inorganic fertilizers and multiple pesticides. This study presents a novel molecular-level structure-activity relationship between Glyp/Ln 3+ and laccase-related efficiency, and provides prerequisites for the risk assessment of contaminants such as Glyp and Ln 3+ in a single system.
Monitoring pesticide residues and lanthanide metals in water system is crucial for safeguarding human health and environmental integrity. Herein, we precisely designed a type of Mn-clay-based composite nanozyme via interfacial engineering and, for the first time, evaluated the effect of the interfacial configuration of glyphosate (Glyp) on laccase-like activity using in-situ ATR-FTIR spectroscopy and mass spectrometry . DFT calculations revealed that the phosphonate group interacts with Mn - Mn to form inner-sphere complexes with multiple configurations tailoring electron transfer and redox ability. Ce 3+ , serving as a hitherto unreported inhibitor of Glyp, can induce the self-coupling consumption of free Glyp, and irreversibly “turn off” latter interfacial laccase-amplification effect by changing its reactive adsorbed configuration. A similar phenomenon can be witnessed in other lanthanide metals (Ln 3+ ), e.g ., La 3+ , Pr 3+ , Nd 3+ , Sm 3+ , Eu 3+ , Gd 3+ , Tb 3+ , Dy 3+ and Er 3+ . With this in mind, an “on-off” enzyme-free nanosensor was designed based on laccase-mimicking cascade catalytic reaction for the selective recognition of Glyp and Ln 3+ . Our nanosensor demonstrates a good linear relationship with Glyp in a wide range of 0–0.2 mM (0–3 μM for Ce 3+ ), with a low LOD of 61 nM for Glyp and 94 nM for Ce 3+ . Additionally, it exhibits high selectivity and anti-interference ability under the co-application of inorganic fertilizers and multiple pesticides. This study presents a novel molecular-level structure-activity relationship between Glyp/Ln 3+ and laccase-related efficiency, and provides prerequisites for the risk assessment of contaminants such as Glyp and Ln 3+ in a single system.
摘要：
Beryllium and its compounds are classified as carcinogens, and prolonged exposure can trigger chronic beryllium disease. The Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling is known to play a critical role in the development and progression of numerous diseases. Leukemia inhibitory factor (LIF), a key upstream cytokine of the JAK-STAT pathway, has been implicated in regulating inflammatory responses and epithelial-mesenchymal transition (EMT) in various diseases. However, the specific involvement of the JAK-STAT pathway and LIF in beryllium sulfate (BeSO₄)-induced EMT in human bronchial epithelial (16HBE) cells remains unclear. To investigate the regulatory mechanisms, we examined the effects of BeSO₄ on 16HBE cells and targeted the JAK-STAT pathway using both pharmacological inhibition (niclosamide) and genetic silencing of LIF. Subsequently, we assessed cell morphology, proliferative capacity, inflammatory protein levels, and EMT marker expression. Our findings demonstrated that BeSO₄ exposure inhibited 16HBE cell proliferation and activated the JAK-STAT pathway. Pretreatment with niclosamide significantly mitigated cellular inflammation and the EMT process induced by BeSO₄. Additionally, silencing LIF markedly reduced JAK-STAT pathway activation and decreased the expression of EMT markers. This study uncovers a novel mechanism underlying BeSO₄-induced EMT in 16HBE cells, providing valuable insights into the molecular mechanisms of toxicity induced by beryllium and its compounds.
摘要：
Dinoflagellates are a group of marine phytoplankton, and many species are considered to be responsible for harmful algal blooms (HABs) due to their toxin production. Nanoplastics (NP) have hazardous effects on algae, but physiological response, toxin production and underlying regulation mechanisms that they induce in dinoflagellates are poorly understood. In this study, a Chinese Alexandrium minutum strain was exposed to various concentrations (0.1, 0.5, 1.0, 5.0, 10.0 and 50.0 mg/L) of polystyrene nanoplastics (PSNP, 50 nm), and its growth, photosynthesis, oxidative stress response, saxitoxin (STX) production, and transcriptomic response were explored in these conditions. The growth and photosynthesis efficiency were inhibited by relative higher concentrations (10.0 and 50.0 mg/L) of PSNP exposure. The chlorophyll a , malondialdehyde (MDA) contents, antioxidant enzyme activities and STX levels gradually increased at low PSNP concentrations and short time exposures, and subsequently decreased at high PSNP concentrations. Most photosynthesis-related genes have similar decreasing trends with physiological changes, while the antioxidant system genes were upregulated to resist the oxidative stress caused under PSNP. The genes involved in N metabolism were upregulated to maintain normal biological activity and synthesize substrates for STX production. In addition, several STX biosynthesis genes were differentially expressed under PSNP treatment. These findings provide a comprehensive understanding of the response mechanisms and STX synthesis in toxin-producing dinoflagellates exposed to PSNP.
Dinoflagellates are a group of marine phytoplankton, and many species are considered to be responsible for harmful algal blooms (HABs) due to their toxin production. Nanoplastics (NP) have hazardous effects on algae, but physiological response, toxin production and underlying regulation mechanisms that they induce in dinoflagellates are poorly understood. In this study, a Chinese Alexandrium minutum strain was exposed to various concentrations (0.1, 0.5, 1.0, 5.0, 10.0 and 50.0 mg/L) of polystyrene nanoplastics (PSNP, 50 nm), and its growth, photosynthesis, oxidative stress response, saxitoxin (STX) production, and transcriptomic response were explored in these conditions. The growth and photosynthesis efficiency were inhibited by relative higher concentrations (10.0 and 50.0 mg/L) of PSNP exposure. The chlorophyll a , malondialdehyde (MDA) contents, antioxidant enzyme activities and STX levels gradually increased at low PSNP concentrations and short time exposures, and subsequently decreased at high PSNP concentrations. Most photosynthesis-related genes have similar decreasing trends with physiological changes, while the antioxidant system genes were upregulated to resist the oxidative stress caused under PSNP. The genes involved in N metabolism were upregulated to maintain normal biological activity and synthesize substrates for STX production. In addition, several STX biosynthesis genes were differentially expressed under PSNP treatment. These findings provide a comprehensive understanding of the response mechanisms and STX synthesis in toxin-producing dinoflagellates exposed to PSNP.
摘要：
Bacterial infectious diseases pose a serious threat to the safety of human life. It is urgent to development a simple, rapid, efficient and safe precise diagnostic methods and synergistic therapy techniques for combating bacteria. Herein, we synthesize CuInSe 2 quantum dots with near-infrared (NIR) emission peak at 1190 nm upon excitation at 808 nm and shifted to 1026 nm after the incorporation of ZnS. Then, Vancomycin was modified on the hydrophilic CuInSe 2 /ZnS quantum dots to obtain a fluorescent nano biological probe (Van-CuInSe 2 /ZnS) that can target Staphylococcus aureus, which was used to monitor the bacterial infection process in vivo in real time. Moreover, the nanosystem possesses excellent photothermal conversion properties, and biocompatibility, which enables precise, targeted NIR-II fluorescent imaging of bacterial inflammation in vivo as well as effectively cure subcutaneous bacterial infection and wound bacterial infection in mice. It shows great potential in the treatment of bacterial infectious diseases.
Bacterial infectious diseases pose a serious threat to the safety of human life. It is urgent to development a simple, rapid, efficient and safe precise diagnostic methods and synergistic therapy techniques for combating bacteria. Herein, we synthesize CuInSe 2 quantum dots with near-infrared (NIR) emission peak at 1190 nm upon excitation at 808 nm and shifted to 1026 nm after the incorporation of ZnS. Then, Vancomycin was modified on the hydrophilic CuInSe 2 /ZnS quantum dots to obtain a fluorescent nano biological probe (Van-CuInSe 2 /ZnS) that can target Staphylococcus aureus, which was used to monitor the bacterial infection process in vivo in real time. Moreover, the nanosystem possesses excellent photothermal conversion properties, and biocompatibility, which enables precise, targeted NIR-II fluorescent imaging of bacterial inflammation in vivo as well as effectively cure subcutaneous bacterial infection and wound bacterial infection in mice. It shows great potential in the treatment of bacterial infectious diseases.
通讯机构：
[Wang, FD ] U;[Min, JX ] Z;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Zhejiang Univ, Affiliated Hosp 2, State Key Lab Expt Hematol, Ferrol Innovat Ctr,Sch Publ Hlth,Sch Med, Hangzhou 310058, Peoples R China.;Zhejiang Univ, Affiliated Hosp 1, Inst Translat Med, Sch Med, Hangzhou 310058, Peoples R China.
关键词：
Iron homeostasis;Ferroptosis;Iron supplementation;Ferrology
摘要：
Iron is indispensable to most lifeforms, underpinning a myriad of physiological processes. Dysregulation of iron homeostasis underlies a broad spectrum of biological phenomena and pathological conditions. Notably, excessive iron overload acts as a key driver of ferroptosis, a unique form of regulated cell death. Consequently, through the lens of ferrology, targeted modulation of iron balance and ferroptosis has emerged as a compelling avenue for the prevention and treatment of major diseases. Herein, we review the molecular mechanisms governing iron homeostasis, the roles of iron metabolism disorders and ferroptosis in disease pathogenesis, and the latest breakthroughs in iron-regulated therapeutic agents.
期刊：
Ecotoxicology and Environmental Safety,2025年290:117580 ISSN：0147-6513
通讯作者：
Cao, Y;Zhou, ZZ
作者机构：
[Cao, Yi; Li, Kuanhang] Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Zhou, Zhengzheng] Southern Med Univ, NMPA Key Lab Safety Evaluat Cosmet, Guangdong Prov Key Lab Trop Dis Res, Dept Hyg Inspection & Quarantine Sci,Sch Publ Hlth, Guangzhou 510515, Guangdong, Peoples R China.
通讯机构：
[Cao, Y ] U;[Zhou, ZZ ] S;Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;Southern Med Univ, NMPA Key Lab Safety Evaluat Cosmet, Guangdong Prov Key Lab Trop Dis Res, Dept Hyg Inspection & Quarantine Sci,Sch Publ Hlth, Guangzhou 510515, Guangdong, Peoples R China.
关键词：
Autophagy;Lipid profiles;Oral toxicity;SiO(2) nanoparticles (NPs);Trace element balance
摘要：
Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influences of orally exposed SiO2 NPs on lipid profiles in gut-liver axis. To this end, we treated mice with 16, 160 or 1600 mg/kg bodyweight SiO2 NPs via intragastric route. After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. Consistently, lipidomics results showed that most of the lipid classes in mouse intestines and livers were decreased following SiO2 NP administration. We further identified the lipid classes significantly decreased in both intestines and livers, such as phosphatidylserine (PS), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Only a few lipid classes, such as anandamide, showed opposite trends in these organs. For metabolism pathway, SiO2 NPs suppressed autophagy, showing as a significant decrease of microtubule-associated protein 1 A/1B light chain 3 (LC3) and adipose triglyceride lipase (Atgl), accompanying with an accumulation of P62, in both intestines and livers. However, lysosomal-associated membrane protein 2 (Lamp2) showed different trend, that it was significantly increased in intestines but decreased in livers. Combined, our results indicated that intragastric administration of SiO2 NPs altered trace element balance and lipid profiles, accompanying with a change of autophagic lipolysis proteins, in mouse gut-liver axis.
Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influences of orally exposed SiO2 NPs on lipid profiles in gut-liver axis. To this end, we treated mice with 16, 160 or 1600 mg/kg bodyweight SiO2 NPs via intragastric route. After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. Consistently, lipidomics results showed that most of the lipid classes in mouse intestines and livers were decreased following SiO2 NP administration. We further identified the lipid classes significantly decreased in both intestines and livers, such as phosphatidylserine (PS), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Only a few lipid classes, such as anandamide, showed opposite trends in these organs. For metabolism pathway, SiO2 NPs suppressed autophagy, showing as a significant decrease of microtubule-associated protein 1 A/1B light chain 3 (LC3) and adipose triglyceride lipase (Atgl), accompanying with an accumulation of P62, in both intestines and livers. However, lysosomal-associated membrane protein 2 (Lamp2) showed different trend, that it was significantly increased in intestines but decreased in livers. Combined, our results indicated that intragastric administration of SiO2 NPs altered trace element balance and lipid profiles, accompanying with a change of autophagic lipolysis proteins, in mouse gut-liver axis.
通讯机构：
[Yang, HF ] U;[Shi, ML ; Cai, R ] H;Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Mol Sci & Biomed Lab,State Key Lab Chemo Biosensin, Changsha 410082, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;Hainan Univ, Sch Mat Sci & Engn, Haikou 570228, Hainan, Peoples R China.
关键词：
carbon dots;cellulose nanofiber;fluorescence detection;nitrite, test strip
摘要：
As a common food additive, nitrites are used in processed meat products, primarily for color enhancement and preservation. Excessive intake of nitrites can lead to high toxicity to pose a significant risk to human health, including potential carcinogenicity and even death. Consequently, developing a rapid and sensitive method for nitrite detection is crucial for food safety monitoring. Herein, this study is developing a nitrite detection system utilizing blue-fluorescent carbon dots (CDs) derived from carboxylate cellulose. This system operates by detecting the quenching of CDs fluorescence, which is caused by Fe 3 ⁺ ions generated via the oxidation of Fe 2 ⁺ by nitrite under acidic conditions. Within a concentration range of 1–1000 µ m , the system demonstrates a strong linear relationship between CDs fluorescence intensity and nitrite concentration, with a detection limit of 0.42 µ m . Furthermore, this study is designing a novel CDs-based fluorescent test strip for rapid and visual nitrite quantification in pakchoi cabbage samples, enabling detection within 30 min. Overall, the CDs-based fluorescent test strips hold great promise for enhancing food safety monitoring, ensuring effective nitrite control, and protecting public health from the potential risks associated with excessive nitrite consumption.
摘要：
Cervical cancer (CC) is a significant global health issue and remains one of the leading causes of cancer-related mortality in women. Radiotherapy is a crucial treatment modality for CC; however, tumor heterogeneity and resistance to radiotherapy often result in suboptimal outcomes for some patients, including recurrence and metastasis. Periostin (POSTN), a matricellular protein within the tumor microenvironment, has been implicated in the promotion of tumor progression and treatment resistance, particularly through mechanisms such as epithelial-mesenchymal transition (EMT). Despite this, the role of POSTN in radiotherapy resistance in CC patients remains underexplored. Therefore, in this study, we investigated the prognostic significance of POSTN expression in CC patients undergoing radical radiotherapy and explored potential mechanisms underlying radiotherapy resistance. We analyzed data from 92 CC patients in The Cancer Genome Atlas (TCGA) and 153 patients from our institution, assessing POSTN expression levels through mRNA analysis and immunohistochemistry (IHC). Our findings revealed that high POSTN expression was significantly associated with advanced tumor stages, poorer radiotherapy outcomes, and worse overall survival (OS). Additionally, multivariate Cox regression analysis identified POSTN as an independent prognostic factor for CC patients undergoing radical radiotherapy. A prognostic nomogram integrating POSTN expression and clinicopathological features demonstrated superior predictive accuracy for OS. Drug sensitivity analysis suggested that high POSTN expression may be linked to resistance to multiple chemotherapeutic agents. Furthermore, weighted correlation network analysis (WGCNA) and gene set enrichment analysis (GSEA) identified EMT as a top enriched pathway in patients with high POSTN expression, suggesting it may play a critical role in radiotherapy resistance. Subsequently, in vitro experiments confirmed that POSTN knockdown significantly inhibited HeLa cell proliferation, invasion, and enhanced radiosensitivity, while promoting apoptosis. These findings indicate that high POSTN expression is a risk factor for poor prognosis in CC patients undergoing radical radiotherapy, and targeting POSTN may improve radiotherapy efficacy by reducing tumor proliferation and resistance.
摘要：
In this research, a new synthesis approach was developed for an adsorbent, namely the phosphorylated ZIF-8/bamboo charcoal/chitosan/tannic acid (P-ZBCT) composite, for the efficient adsorption of uranyl ions from wastewater at low dosages. Impressively, the uranium adsorption rate of P-ZBCT reaches up to 98 % at a low dosage of 0.056 g/L in a 10-mg/L uranium solution, outperforming most reported uranium adsorption materials. The theoretical maximum adsorption capacity of P-ZBCT for uranium at 308 K and pH 6.0 is 2357.69 mg/g, with uranium adsorption being a spontaneous endothermic chemical reaction. Mechanistic analysis reveals that surface functional groups such as P O, amino group, and C N play a pivotal role in uranium adsorption. A competitive adsorption experiment shows that zinc is the most competitive with uranium adsorption; however, the partition coefficient of U is 11 times that of zinc, indicating that the absorption of uranium is more selective than that of other metal ions, such as zinc. Adsorption treatment using P-ZBCT successfully reduces the uranium content in real uranium tailings–containing pond wastewater to 34 μg/L. P-ZBCT demonstrates exceptional recycling performance, maintaining an adsorption rate of 85 % even after 10 sorption–desorption cycles. Therefore, P-ZBCT exhibits significant potential for efficiently extracting uranium from low-concentration uranium-containing wastewater.
In this research, a new synthesis approach was developed for an adsorbent, namely the phosphorylated ZIF-8/bamboo charcoal/chitosan/tannic acid (P-ZBCT) composite, for the efficient adsorption of uranyl ions from wastewater at low dosages. Impressively, the uranium adsorption rate of P-ZBCT reaches up to 98 % at a low dosage of 0.056 g/L in a 10-mg/L uranium solution, outperforming most reported uranium adsorption materials. The theoretical maximum adsorption capacity of P-ZBCT for uranium at 308 K and pH 6.0 is 2357.69 mg/g, with uranium adsorption being a spontaneous endothermic chemical reaction. Mechanistic analysis reveals that surface functional groups such as P O, amino group, and C N play a pivotal role in uranium adsorption. A competitive adsorption experiment shows that zinc is the most competitive with uranium adsorption; however, the partition coefficient of U is 11 times that of zinc, indicating that the absorption of uranium is more selective than that of other metal ions, such as zinc. Adsorption treatment using P-ZBCT successfully reduces the uranium content in real uranium tailings–containing pond wastewater to 34 μg/L. P-ZBCT demonstrates exceptional recycling performance, maintaining an adsorption rate of 85 % even after 10 sorption–desorption cycles. Therefore, P-ZBCT exhibits significant potential for efficiently extracting uranium from low-concentration uranium-containing wastewater.
摘要：
Pseudomonas aeruginosa poses a significant clinical challenge due to its intrinsic and acquired antimicrobial resistance and robust biofilm formation, which complicates treatment. Bacteriophages (phages), viruses targeting bacteria, are emerging as a promising alternative or adjunct to combat multidrug-resistant P. aeruginosa infections. This review systematically examines the taxonomic diversity of phages infecting P. aeruginosa, with emphasis on those that remain unclassified at the family taxonomic level, such as Pbunavirus and Pakpunavirus. It comprehensively synthesizes current knowledge on phage receptor-binding proteins (RBPs) - the molecular determinants of host specificity - and their corresponding receptors on the P. aeruginosa surface, such as lipopolysaccharide (LPS), pili, flagella, outer membrane proteins, and alginate. Critically, the review underscores the urgent need to decipher the precise molecular mechanisms governing RBP-receptor interactions. A deeper understanding of these specific recognition events is paramount. This knowledge is essential not only for rationally optimizing phage therapy efficacy, including through engineered phages or RBP-based antimicrobials, but also for developing highly sensitive and specific phage-derived diagnostic tools, utilizing whole phages or purified RBPs as recognition elements for rapid P. aeruginosa detection.
摘要：
Radiation-induced intestinal injury (RIII) is a significant concern for cancer patients receiving radiation therapy, as it can lead to complications such as radiation enteropathy. Presently, there are limited options for preventing or treating RIII. Tea polyphenols (TP), found in tea, provide various health benefits, but their antiradiation mechanisms are not fully understood. C57BL/6 mice pre-treated with TP for five days showed a significant improvement in survival rates after being exposed to 10 Gy of 60Co radiation. In the same way, abdominal exposure to 15 Gy of 60Co radiation effectively mitigated radiation-induced colon shortening, damage to intestinal tissues, oxidative stress, the release of inflammatory factors, and disruptions in intestinal microbial balance. In addition, TP treatment lowered the elevation of reactive oxygen species (ROS), iron imbalance, mitochondrial damage, and ferroptosis in IEC-6 cells post-irradiation. Utilizing network pharmacology, molecular docking, and affinity testing, we identified that TP has the capability to target the Nrf2/HO-1/GPX4 signaling pathway, while EGCG, a principal constituent of TP, interacts with HSP90 and mitigates radiation-induced ferroptosis. These findings suggest that TP may serve as a promising therapeutic agent to alleviate radiation-induced intestinal injury (RII).
摘要：
BACKGROUND: This retrospective multicenter study is aimed at evaluating the diagnostic accuracy and influence factors of serum des-gamma-carboxy prothrombin (DCP) as a diagnostic biomarker of hepatocellular carcinoma (HCC). METHODS: Clinical data were collected from 4555 subjects with DCP tests, composed of primary liver cancer (PLC), metastatic liver cancer (MLC), chronic hepatitis (CH), liver cirrhosis (LC), benign liver diseases (BLD), biliary tract diseases (BTD), non-liver cancers (NLC), and non-liver benign diseases (NLBD). The clinical data collected included medical history, treatment records, various serum tests, and imaging examination. RESULTS: Serum DCP was measured with Abbott agents in each center. In HCC, serum DCP concentration was at 9086.00 ± 366.10 mAU/mL, higher than that in other diseases (p < 0.05). At 40.00 mAU/mL recommended by instruction, positive rates of serum DCP were at 85.11% in HCC, 30.12% in intrahepatic cholangiocellular carcinoma (ICC), 31.65% in MLC, 13.95% in BLD, 18.14% in CH, 27.87% in LC, 15.75% in BTD, 35.29% in NLC, and 20.00% in NLBD. In this study, the diagnostic specificity of serum DCP in HCC was affected by liver function. In HCC, serum AFP concentrations also increased compared to non-HCC diseases (p < 0.05), but specificity varied with agents from different providers. Serum DCP decreased after the surgical removal of HCC, but remained elusive in systemic treatment. CONCLUSION: Serum DCP may serve as an optimal biomarker for the diagnosis of HCC, but its accuracy appears influenced by liver function; attention needs to be paid to the liver function of patients for false positivity.
作者机构：
[Zhao, Le; Chen, Dong-Yang] School of Public Health，University of South China，Hengyang 421001，China;[Cai, Liang; Liu, Xian-Jun; Chen, Dong-Yang; Fan, Xiang; Li, Tan-Yao; Zhang, Hao; Li, Jian] Hunan Provincial Center for Disease Control and Prevention （Hunan Academy of Preventive Medicine），Changsha 410153，China
通讯机构：
[Chen, DY ] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Hunan Acad Prevent Med, Hunan Prov Ctr Dis Control & Prevent, Changsha 410153, Peoples R China.
关键词：
QuEChERS;pesticide residues;risk assessment;ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）
摘要：
Pesticide residues in fruits and vegetables are becoming a serious issue. These residues can affect the quality of agricultural products and people's health. Therefore， it has become crucial to effectively monitor and control pesticide residues in the food safety field. In this study， a rapid and effective QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry （QuEChERS-UPLC-MS/MS） method was established for the simultaneous determination of 79 typical pesticides in vegetables and fruits， including organophosphates， carbamates， and pyrethroids. The pretreatment， UPLC， and MS/MS conditions were optimized. The fruit and vegetable samples were extracted with frozen acetonitrile after pulverization and homogenization， cleaned up by the QuEChERS method， filtered through a centrifugal membrane， and analyzed by UPLC-MS/MS. The separation was carried out on an ACQUITY UPLC HSS T3 column （100 mm×2.1 mm， 1.8 μm） with gradient elution. The aqueous and organic phases were water-methanol （98∶2， v/v） and methanol-water （98∶2， v/v） respectively， both with 5 mmol/L ammonium acetate and 0.1% formic acid. A triple quadrupole mass spectrometer was used in positive-ion electrospray ionization （ESI(+)） scanning mode， with target pesticide residues quantified using the matrix-matched standard-curve method. The results showed that under the optimized conditions， the 79 target compounds were determined with good linearities in the range of 0.1-200 μg/L， and the correlation coefficients （r） were all greater than 0.990. The limits of detection （LODs） and limits of quantification （LOQs） of the 79 compounds were in the range of 0.01-4.0 μg/kg and 0.03-13.0 μg/kg. The recoveries at three spiked levels ranged from 78.2% to 119.8%， with relative standard deviations （RSDs） all less than 15.8%. The established method was successfully applied to 80 samples of fruits and vegetables from Hunan province. As a result， 19 pesticides were detected in 31 samples， and thiamethoxam， acetamiprid and clothianidin being the most highly detected with a content range of 0.012-2.62 mg/kg； According to the data of the Hunan province survey yearbook， the percentages of acceptable daily intake （%ADI） for chronic dietary exposure of the detected neonicotinoid insecticides （thiamethoxam and clothianidin） have been calculated. The results indicate that the %ADI of clothianidin in fruits and vegetables ranged from 5.74% to 0.36%， respectively， and the %ADI of thiamethoxam in fruits and vegetables ranged from 0.40% to 19.50%. The %ADI of both pesticides were found to be less than 100%， indicating they are within acceptable limits. The method is simple， sensitive， accurate， and suitable for the simultaneous determination of multiple pesticide residues in fruits and vegetables.
摘要：
Since the revelation of Fe 3 O 4 nanoparticles with enzyme-like characteristics, nanozymes present a promising alternative to traditional bio-enzymes due to their straightforward preparation, good robustness, and adjustable performance. In addition to enzyme-mimetic catalysis, these materials also tend to show optical, electrical, thermal, and magnetic properties. Integrating nanozyme catalysis with these properties into an entity can offer fascinating merits to biochemical sensing. Especially, fluorescent nanozymes, a class of materials combining enzyme-mimicking activity and fluorescence, have been drawing growing interest in the analytical chemistry community in the past five years. To highlight the achievements and progress made in this emerging area, here we, for the first time, present a comprehensive summary on fluorescent nanozymes and their roles in advancing analytical detection. First, a definition of fluorescent nanozymes is given, and their bifunctional features are discussed. Then, common strategies employed to develop different material types of fluorescent nanozymes are introduced. Emphatically, their unique contributions to biochemical analysis are clarified in detail, along with various applications in environmental monitoring, food safety analysis, and biomedical detection. Finally, substantial potential of fluorescent nanozymes in realizing advanced detection as well as existing challenges is spotlighted. Our review provides a comprehensive and up-to-date reference on fluorescent nanozymes, which will attract more attention and guide future efforts to further promote the promising field.
Since the revelation of Fe 3 O 4 nanoparticles with enzyme-like characteristics, nanozymes present a promising alternative to traditional bio-enzymes due to their straightforward preparation, good robustness, and adjustable performance. In addition to enzyme-mimetic catalysis, these materials also tend to show optical, electrical, thermal, and magnetic properties. Integrating nanozyme catalysis with these properties into an entity can offer fascinating merits to biochemical sensing. Especially, fluorescent nanozymes, a class of materials combining enzyme-mimicking activity and fluorescence, have been drawing growing interest in the analytical chemistry community in the past five years. To highlight the achievements and progress made in this emerging area, here we, for the first time, present a comprehensive summary on fluorescent nanozymes and their roles in advancing analytical detection. First, a definition of fluorescent nanozymes is given, and their bifunctional features are discussed. Then, common strategies employed to develop different material types of fluorescent nanozymes are introduced. Emphatically, their unique contributions to biochemical analysis are clarified in detail, along with various applications in environmental monitoring, food safety analysis, and biomedical detection. Finally, substantial potential of fluorescent nanozymes in realizing advanced detection as well as existing challenges is spotlighted. Our review provides a comprehensive and up-to-date reference on fluorescent nanozymes, which will attract more attention and guide future efforts to further promote the promising field.
摘要：
The success of graphene oxides has gained extensive research interests in developing novel 2D nanomaterials (NMs). WS(2) nanosheets (NSs) are novel transition metal-based 2D NMs, but their toxicity is unclear. In this study, we investigated the oral toxicity of WS(2) NSs to mouse intestines. Male mice were administrated with vehicles, 1, 10, or 100 mg/kg NSs via intragastric route, once a day, for 5days. The results indicate that the NSs did not induce pathological or ultrastructural changes in intestines. There were minimal changes of trace elements that the exposure did not induce W accumulation, and only Co levels were dose-dependently increased. Lipid droplets were observed in all groups of mice, but lipidomics data indicate that WS(2) NSs only significantly decreased four lipid species, all belonging to phosphatidylcholine (PC). The levels of proteins regulating autophagic lipolysis, namely, LC3, lysosomal associated membrane protein 2 (LAMP2) and perilipin 2 (PLIN2), were increased, but it was only statistically significantly different for LC3. The results of this study suggest that repeated intragastric exposure to WS(2) NSs only induced minimal influences on pathological injury, trace element balance, autophagy, and lipid profiles in mouse intestines, indicating relatively high biocompatibility of WS(2) NSs to mouse intestine via oral route.
