作者机构：
[Luo, Jiayou; Pan, Xiongfeng] Pediatrics Research Institute of Hunan Province, Hunan Children's Hospital, Changsha, China;[Luo, Jiayou; Yao, Zhenzhen; Pan, Xiongfeng; Luo, Miyang; Dai, Wen; Xiao, Xiang; Wei, Jia; Duan, Yamei; Ouyang, SiSi; Liu, Yixu] Department of Maternal and Child Health, Xiangya School of Public Health, Central South University, Changsha, China;[Luo, Miyang] Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, Changsha, China;[Luo, Miyang] Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore;[Kaminga, Atipatsa C] Department of Mathematics and Statistics, Mzuzu University, Mzuzu, Malawi
摘要：
Altered gut microbiota and metabolites are important for non-alcoholic fatty liver disease (NAFLD) in children. We aimed to comprehensively examine the effects of gut metabolites on NAFLD progression. We performed integrative metabolomics (untargeted discovery and targeted validation) analysis of non-alcoholic fatty liver (NAFL), non-alcoholic steatohepatitis (NASH), and obesity in children. Fecal samples were collected from 75 subjects in the discovery cohort (25 NAFL, 25 NASH, and 25 obese control children) and 145 subjects in an independent validation cohort (53 NAFL, 39 NASH, and 53 obese control children). Among 2,491 metabolites, untargeted metabolomics revealed a complete NAFLD metabolic map containing 318 increased and 123 decreased metabolites. Then, machine learning selected 65 important metabolites that can distinguish the severity of the NAFLD. Furthermore, precision-targeted metabolomics selected 5 novel gut metabolites from 20 typical metabolites. The functionality of candidate metabolites was validated in hepatocyte cell lines. In the end, this study annotated two novel elevated pathogenic metabolites (dodecanoic acid and creatinine) and a relationship between depleted protective gut microbiota (Butyricicoccus and Alistipes), increased inflammation (IL-1β), lipid metabolism (TG), and liver function (ALT and AST). This study demonstrates the role of novel gut metabolites (dodecanoic acid and creatinine), as the fatty acid metabolism regulator contributing to NAFLD development through its influence on inflammation and liver function. IMPORTANCE: Altered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota (Butyricicoccus and Alistipes) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development.
作者机构：
[Fu, Lichun; Zhen, Deshuai; Yang, Jing; Xiong, Lihao; Luo, Chunhua] Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;[Zhen, Deshuai; Xiong, Lihao; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Gao, Linxiao; Zhen, Deshuai; Luo, Xiaohu] Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.;[Zhou, Huang] Huaihua Univ, Coll Chem & Mat Engn, Huaihua 418000, Peoples R China.;[Yang, Lixia] Nanchang Hangkong Univ, Key Lab Jiangxi Prov Persistent Pollutants Control, Nanchang 330063, Peoples R China.
通讯机构：
[Gao, LX] Q;[Zhen, DS ] H;Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.
关键词：
Yb@TiO2;Perfluorooctane Sulfonate;Tetrabromobisphenol A;Contaminants;SELDI-TOF MS
摘要：
Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol-gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg center dot mL-1. The results of the analysis of camellia oil samples show good recovery (80.3%-86.8%) with a low detection limit for TBBPA.
摘要：
Halloysite nanotubes (HNTs) are nanomaterials (NMs) derived from natural clays and have been considered as biocompatible NMs for biomedical uses. However, the cardiovascular toxicity of HNTs has not been thoroughly investigated. In this study, we compared the cardiotoxicity of HNTs and multi-walled carbon nanotubes (MWCNTs), focusing on the changes in Kruppel-like factor (KLF)-mediated signaling pathways. Mice were intravenously injected with 50µg NMs, once a day, for 5 days, and then mouse hearts were removed for experiments. While HNTs or MWCNTs did not induce obvious pathological changes, RNA-sequencing data suggested the alterations of KLF gene expression. We further confirmed an increase of Klf15 positive cells, accompanied by changes in Klf15-related gene ontology (GO) terms. We noticed that most of the changed GO terms are related with the regulation of gene expression, and we confirmed that the NMs increased myoneurin (Mynn) but decreased snail family transcriptional repressor 1 (Snai1), two transcription factors (TFs) related with Klf15. Besides, the changed GO terms also include metal ion binding and positive regulation of glucose import, and we verified an increase of phosphoenolpyruvate carboxykinase 1 (Pck1) and insulin receptor (Insr). However, HNTs and MWCNTs only showed minimal impact on cell death signaling pathways, and no increase in apoptotic sites was observed after NM treatment. We concluded that intravenous administration of HNTs and MWCNTs activated a protective TF, namely Klf15 in mouse aortas, to alter gene expression and signaling pathways related with metal ion binding and glucose import.
作者机构：
[Yufei Lei; Qi Li; Qian Li; Dongxiu He; Jinhua Xue; Lifu Liao; Jikai Wang] School of Chemistry and Chemical Engineering, School of Pharmaceutical Science, School of Public Health, Hunan Key Laboratory for the Design and Application of Actinide Complexes, University of South China, Hengyang, Hunan 421001, P. R. China;State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, P. R. China;[Xilin Xiao] School of Chemistry and Chemical Engineering, School of Pharmaceutical Science, School of Public Health, Hunan Key Laboratory for the Design and Application of Actinide Complexes, University of South China, Hengyang, Hunan 421001, P. R. China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, P. R. China
通讯机构：
[Jikai Wang; Xilin Xiao] S;School of Chemistry and Chemical Engineering, School of Pharmaceutical Science, School of Public Health, Hunan Key Laboratory for the Design and Application of Actinide Complexes, University of South China, Hengyang, Hunan 421001, P. R. China<&wdkj&>School of Chemistry and Chemical Engineering, School of Pharmaceutical Science, School of Public Health, Hunan Key Laboratory for the Design and Application of Actinide Complexes, University of South China, Hengyang, Hunan 421001, P. R. China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, P. R. China
摘要：
In this paper, nitrogen-doped carbon dots (NCDs) were synthesized via a facile hydrothermal method using gum arabic (GA) and tris(hydroxymethyl)methyl aminomethane (tris) as precursors. The resultant NCDs show uniform size distribution and stable optical performance. Then a fluorescent signal quenching probe based on NCDs was proposed for the quantitative detection of mercury ions (Hg2+). The developed fluorescent nanoprobe based on NCDs demonstrated a good linear relationship in the range of 10 to 120 μM (R2 = 0.99265) for toxic mercury(II), with a detection limit of 4.87 μM. Additionally, the present assay system could be efficiently used for the detection of Hg2+ in real water samples with a satisfactory recovery rate (96.11–104.04%). The metal-free NCDs, which exhibited little cytotoxicity, were successfully applied to in vitro fluorescence imaging of human triple negative breast cancer (TNBC) cells, achieving visualization of Hg2+ in cells. With good water solubility and biocompatibility, the nitrogen-doped carbon dots synthesized by this economical and ecologically friendly process are suitable for use in analytical testing and biological research.
摘要：
Multiple pesticides are often used in combination for plant protection and public health. Therefore, it is important to analyze the physiological changes induced by multiple pesticides exposure. The objective of this study was to investigate the combined toxicity of the widely-used organophosphorus and pyrethroid pesticides diazinon, dimethoate, and cypermethrin. Male Wistar rats were administrated by gavage once daily with the three pesticides individual or in combination for consecutive 28 days. The metabolic components of serum and urine samples were detected by using 1H nuclear magnetic resonance (NMR)-based metabolomics method. Histopathological examination of liver and kidneys and serum biochemical determination were also carried out. The results showed that after the 28-day subacute exposure, serum glutamic transaminase and albumin were significantly increased and blood urea nitrogen was significantly decreased in the rats exposed to the mixture of the pesticides compared with the control rats, suggesting that the co-exposure impaired liver and kidney function. Metabolomics analysis indicated that the indicators 14 metabolites were statistically significant altered in the rats after the exposure of the pesticides. The increase in 3-hydroxybutyric acid in urine or decrease of lactate and N-acetyl-L-cysteine in serum could be a potentially sensitive biomarker of the subchronic combined effects of the three insecticides. The reduction level of 2-oxoglutarate and creatinine in urine may be indicative of dysfunction of liver and kidneys. In summary, the exposure of rats to pesticides diazinon, dimethoate, and cypermethrin could cause disorder of lipid and amino acid metabolism, induction of oxidative stress, and dysfunction of liver and kidneys, which contributes to the understanding of combined toxic effects of the pesticides revealed by using the metabolomics analysis of the urine and serum profiles.
摘要：
Given that intricate toxicological profiles exist among different antibiotics and pose serious threats to the environment and human health, synchronous analysis of multiple residues becomes crucial. Sensor arrays show potential to achieve the above purpose, but it is challenging to develop easy-to-use and high-sensitivity tools because the state-of-the-art arrays often require more than one recognition unit and are monosignal dependent. Here we exquisitely designed a fluorescent nanoprobe (2-aminoterephthalic acid-anchored CdTe quantum dots with Eu3+ coordination, CdTe-ATPA-Eu3+) featuring triple emissions at the same excitation as the only element to fabricate a luminescent sensor array with ratiometric calculations for identifying multiple antibiotics. By taking tetracycline, chlortetracycline, doxycycline, oxytetracycline, penicillin G, and sulfamethoxazole as models, the six species exhibited distinguishable motivation or/and quenching impacts on the three emissions of CdTe-ATPA-Eu3+, which were employed as indicators to perform the ratiometric logical operation and further combined with pattern recognition analysis for multitarget determination. Evidently, such a design exhibits two advances: (1) with the triple-emission probe as the sole receptor requiring neither internal nor external adjustments, the fabricated array acts as an extremely facile tool for multianalyte detection; (2) the ratiometric calculations offer excellent sensitivity and reliability for high-performance determination. Consequently, accurate identification and quantification of individual antibiotics and their combinations at various levels were verified in both laboratory and practical matrices. Our work provides a new tool for simultaneously detecting multiple antibiotics, and it will inspire the development of advanced sensor arrays for multitarget analysis.
通讯机构：
[Yang, SY ] U;Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Hunan, Peoples R China.
关键词：
Cobalt -nitrogen co -doped carbon dots;Colorimetric;Fluorometric;Dual -mode;Gallic acid
摘要：
Gallic acid (GA), widely used in food and medicine production industries. Herein, a kind of newly synthesized cobalt-nitrogen co-doped carbon dots (Co,N-CDs) with dual functional properties of oxidase-like activity and photoluminescence property could oxidize the 3,3 ',5,5 '-tetramethylbenzidine (TMB) to oxTMB with a new ab-sorption peak at 653 nm. In addition, it was found that oxTMB performed a fluorescence-quenching effect on Co, N-CDs at 440 nm attributed to FRET (Fluorescence resonance energy transfer). Reductive GA could reduce oxTMB to TMB, weaken the blue color, and restore the fluorescence of Co,N-CDs. Thus, a colorimetric and fluorometric method for dual-mode detection of GA was established. The synthesis of the dual-signal probe was completed with a single and environmental precursor in just one step. Simultaneously, the dual-mode detection reduced detection limits, broadened the linear range and improved anti-interference ability. This method exhibited a satisfactory application prospect in the actual detection of GA in tea beverages.
期刊：
International Journal of Biological Macromolecules,2024年:129220 ISSN：0141-8130
通讯作者：
Dai, Homg;Xie, Zhi-Hui;Liu, Yali
作者机构：
[Guo, Meng; Zhen, Deshuai; Luo, Xiaohu; Deng, Qiuhui] Engineering Research Center of Loss Efficacy and Anticorrosion of Materials of Guizhou, School of Chemistry and Chemical Engineering, Qiannan Normal University for Nationalities, Duyan 558000, PR China;[Luo, Xiaohu; Deng, Qiuhui] State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China;[Zhen, Deshuai] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China;[Zhen, Deshuai] State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China;[Mao, Haili] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China
通讯机构：
[Dai, Homg] E;[Xie, Zhi-Hui] C;[Liu, Yali] S;Engineering Research Center of Loss Efficacy and Anticorrosion of Materials of Guizhou, School of Chemistry and Chemical Engineering, Qiannan Normal University for Nationalities, Duyan 558000, PR China. Electronic address:;Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, PR China. Electronic address:
摘要：
In this work, a total of 18 types of choline chloride, betaine, and L-proline-based deep eutectic solvents (DESs) were synthesized to determine the extraction yield of a natural polysaccharide (PSA) from Dysosma versipellis using an ultrasound-assisted extraction method. Results indicate that the choline-oxalic acid-based DES has the best extraction yield for PSA due to the proper physical-chemical properties between PSA and DES. To evaluate the optimal extraction conditions, a response surface methodology was carried out. Under the optimal conditions, the extraction yield of PSA reaches 10.37 % (± 0.03 %), higher than the conventional extraction methods. Findings from FT-IR and NMR suggest that the extracted PSA belongs to a neutral polysaccharide with (1 → 6)-linked α-d-glucopyranose in the main chain. Interestingly, results from various electrochemical measurements show the extracted PSA exhibits excellent corrosion inhibition performance for mild steel (MS) in a 0.5 M HCl solution, with 90.8 % of maximum corrosion inhibition efficiency at 210 mg L(-1). SEM and XPS measurements reveal the formation of a protective layer on the MS surface. The adsorption behaviour of extracted PSA well obeys the Langmuir adsorption isotherm containing the chemisorption and physisorption. Additionally, theoretical calculations validate the experimental findings.
作者机构：
[Wang, Jingyu; Bai, Qinqin; Liang, H; Zheng, Yi; Zhao, Fengxia; Liang, Hao; Yan, Hangli; Wu, Linghao; Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.;[Hu, Hongmei] Hengyang Ctr Dis Control & Prevent, Hengyang 421001, Peoples R China.
通讯机构：
[Liang, H ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.
摘要：
The detection of foodborne pathogens is crucial for food hygiene regulation and disease diagnosis. Colorimetry has become one of the main analytical methods in studying foodborne pathogens due to its advantages of visualization, low cost, simple operation, and no complex instrument. However, the low sensitivity limits its applications in early identification and on-site detection for trace analytes. In order to overcome such a limitation, herein we propose a joint strategy featuring dual signal amplification based on the hybridization chain reaction (HCR) and DNA-enhanced peroxidase-like activity of gold nanoparticles (AuNPs) for the sensitive visual detection of Escherichia coli. Target bacteria bound specifically to the aptamer domain in the capture hairpin probe, exposing the trigger domain for HCR and forming the extended double-stranded DNA (dsDNA) structures. The peroxidase-like catalytic capacity of AuNPs can be enhanced significantly by dsDNAs with the sticky ends of dsDNAs being adsorbed on AuNPs and the rigidity of dsDNAs causing the spatial regulation of AuNP concentration. The intensity of the enhancement was linearly related to the number of target bacteria. With the above strategy, the detection limit of our colorimetric method for Escherichia coli was down to 28 CFU mL(-1) within a short analytical time (50 min). This study provides a new perspective for the sensitive and visual detection of early bacterial contamination in foods.
作者机构：
[Min, Junxia; Yu, Yingying; Wang, Fudi; Liu, Yutong; Zhou, Jiahui; Yue, Wuyang; Su, Yunxing; Yang, Sisi; Li, Xiaopeng; Min, JX; Sun, Shumin] Zhejiang Univ, Affiliated Hosp 1, Affiliated Hosp 2, Inst Translat Med,Sch Med, Hangzhou 310058, Peoples R China.;[Yu, Yingying; Wang, Fudi; Lin, Zhiting] Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Shah, YM; Shah, Yatrik M.; Das, Nupur K.] Univ Michigan, Div Gastroenterol, Internal Med, Ann Arbor, MI 48109 USA.;[Shah, YM; Shah, Yatrik M.; Das, Nupur K.] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA.;[Wu, Qian] Zhejiang Univ, Int Inst Med, Sch Med, Yiwu, Zhejiang, Peoples R China.
通讯机构：
[Wang, FD ; Min, JX] Z;[Shah, YM ] U;Zhejiang Univ, Affiliated Hosp 1, Affiliated Hosp 2, Inst Translat Med,Sch Med, Hangzhou 310058, Peoples R China.;Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Univ Michigan, Div Gastroenterol, Internal Med, Ann Arbor, MI 48109 USA.
关键词：
FG-4592;HIF;anemia of inflammation;chemotherapy-induced anemia;hypoxia;iron-refractory iron deficiency anemia
摘要：
In clinics, hepcidin levels are elevated in various anemia-related conditions, particularly in iron-refractory anemia and in high inflammatory states that suppress iron absorption, which remains an urgent unmet medical need. To identify effective treatment options for various types of iron-refractory anemia, the potential effect of hypoxia and pharmacologically-mimetic drug FG-4592 (Roxadustat) are evaluated, a hypoxia-inducible factor (HIF)-prolyl hydroxylase (PHD) inhibitor, on mouse models of iron-refractory iron-deficiency anemia (IRIDA), anemia of inflammation and 5-fluorouracil-induced chemotherapy-related anemia. The potent protective effects of both hypoxia and FG-4592 on IRIDA as well as other 2 tested mouse cohorts are found. Mechanistically, it is demonstrated that hypoxia or FG-4592 could stabilize duodenal Hif2 alpha, leading to the activation of Fpn transcription regardless of hepcidin levels, which in turn results in increased intestinal iron absorption and the amelioration of hepcidin-activated anemias. Moreover, duodenal Hif2 alpha overexpression fully rescues phenotypes of Tmprss6 knockout mice, and Hif2 alpha knockout in the gut significantly delays the recovery from 5-fluorouracil-induced anemia, which can not be rescued by FG-4592 treatment. Taken together, the findings of this study provide compelling evidence that targeting intestinal hypoxia-related pathways can serve as a potential therapeutic strategy for treating a broad spectrum of anemia, especially iron refractory anemia. In this article, it is demonstrated that targeting the duodenal Hif2 alpha-Fpn axis as a novel strategy to improve refractory hepcidin-activated anemias, including iron-refractory iron-deficiency anemia (IRIDA), inflammatory anemia and chemotherapy-induced anemia, in mice, which provides compelling evidence for further clinical translation.image
期刊：
European Journal of Pharmacology,2024年:176481 ISSN：0014-2999
通讯作者：
Zhao, Xiao-Mei;Jiang, Miao
作者机构：
[He, Xueke; Zhou, Yangyang; Peng, Weiqiang; Liao, Minjun] Institute of Cardiovascular Disease, Department of Pathophysiology, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Hengyang Medical College, University of South China, Hengyang, 421001, PR China;[Zhou, Yangyang; Peng, Weiqiang; Liao, Minjun] Department of Clinical Medicine, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, PR China;[Zhao, Xiao-Mei] College of Public Health, University of South China, Hengyang, 421001, Hunan, PR China. Electronic address: mavis1015@163.com;[Jiang, Miao] Institute of Cardiovascular Disease, Department of Pathophysiology, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Hengyang Medical College, University of South China, Hengyang, 421001, PR China. Electronic address: miao_jiang@usc.edu.cn
通讯机构：
[Jiang, Miao] I;[Zhao, Xiao-Mei] C;College of Public Health, University of South China, Hengyang, 421001, Hunan, PR China. Electronic address:;Institute of Cardiovascular Disease, Department of Pathophysiology, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Hengyang Medical College, University of South China, Hengyang, 421001, PR China. Electronic address:
摘要：
Atherosclerotic disease is a chronic disease that predominantly affects the elderly and is the most common cause of cardiovascular death worldwide. Atherosclerosis is closely related to processes such as abnormal lipid transport and metabolism, impaired endothelial function, inflammation, and oxidative stress. Coenzyme Q10 (CoQ10) is a key component of complex Ⅰ in the electron transport chain and an important endogenous antioxidant that may play a role in decelerating the progression of atherosclerosis. Here, the different forms of CoQ10 presence in the electron transport chain are reviewed, as well as its physiological role in regulating processes such as oxidative stress, inflammatory response, lipid metabolism and cellular autophagy. It was also found that CoQ10 plays beneficial effects in atherosclerosis by mitigating lipid transportation, endothelial inflammation, metabolic abnormalities, and thrombotic processes from the perspectives of molecular mechanisms, animal experiments, and clinical evidence. Besides, the combined use of CoQ10 with other drugs has better synergistic therapeutic effects. It seems reasonable to suggest that CoQ10 could be used in the treatment of atherosclerotic cardiovascular diseases while more basic and clinical studies are needed.
摘要：
Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) persistently kills nearly 1.5 million lives per year in the world, whereas the only licensed TB vaccine BCG exhibits unsatisfactory efficacy in adults. Taking BCG as a vehicle to express Mtb antigens is a promising way to enhance its efficacy against Mtb infection. In this study, the immune efficacy of recombination BCG (rBCG-ECD003) expressing specific antigens ESAT-6, CFP-10, and nDnaK was evaluated at different time points after immunizing BALB/c mice. The results revealed that rBCG-ECD003 induced multiple Th1 cytokine secretion including IFN-γ, TNF-α, IL-2, and IL-12 when compared to the parental BCG. Under the action of PPD or ECD003, rBCG-ECD003 immunization resulted in a significant increase in the proportion of IL-2(+) and IFN-γ(+)IL-2(+) CD4(+)T cells. Importantly, rBCG-ECD003 induced a stronger long-term humoral immune response without compromising the safety of the parental BCG vaccine. By means of the protective efficacy assay in vitro, rBCG-ECD003 showed a greater capacity to inhibit Mtb growth in the long term. Collectively, these features of rBCG-ECD003 indicate long-term protection and the promising effect of controlling Mtb infection.
摘要：
Uranium, regarded as an important element in nuclear fission energy schemes, is harmful to public health owing to its high toxicity and radioactivity. Herein, a novel fluorescent probe (SC4A@Tb3+) was developed using a one-step liquid-phase method with 4-sulfocalix[4]arene (SC4A) and rare earth-metal ions (Tb3+) as precursors to realize the accurate monitoring of uranyl ion (UO22+) detection. Based on the dynamic quenching mechanism, the SC4A@Tb3+ fluorescence quenching sensor for UO22+ detection was in the linear range of 0-0.36 mu M, with a detection limit of 20 mu g center dot L-1, and successfully quantified UO22+ in water and human serum samples. These results suggest that SC4A@Tb3+ is a potential sensing material for UO22+ detection.
摘要：
How to fabricate a multifunctional coating with controllable ultralow refractive index (RI), high antifogging and anticorrosion performances by a facile method is still one of the great challenges. In this work, a simple and time-saving approach involving a microwave-assisted sol-sel process was developed to fabricate a novel core-shell structured silica nanoparticle sol by co-condensation of perfluorodecyltriethoxysilane (F-DTOS) and tetraethoxysilane (TEOS) and self-condensation of F-DTOS. Results suggest that both the co-condensations and self-condensations not only give a layer of low surface energy -C10H4F17 groups on the silica particles but also enlarge the hybrid silica particle size, providing more and larger voids to form the high porosity. After spinning the silica nanoparticles on the substrate, the obtained optical coating exhibits the controllable ultralow refractive index (RI) and outstanding transparency. The minimum RI and maximum transmittance of our developed coating is up to similar to 1.04 and similar to 100 %, respectively. Moreover, because of the high porosity and the presence of low surface energy -C10H4F17 groups in the coating, the coating has the high hydrophobicity (water contact angle similar to 108.8 degrees), which not only makes it excellent antifogging performance but also affords it high-efficient anticorrosion property. After immersion in a 3.5 wt% NaCl solution for 7 days, the R-ct value of the coating could be higher than 7.3 x 10(4) Omega cm(2). It is believed that our developed method used to fabricate such multifunctional coating does not need any costly equipments, complex steps and time-consuming, making it well suited for industrial application.
作者机构：
[Liu, Zijing; Yang, Shengyuan; Cai, Yujiao; Yang, Hongfen; Liu, Jinquan; Wang, Wenjuan; Chen, Danrong] Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China. yangshyhy@126.com;[Liu, Zijing; Yang, Shengyuan; Cai, Yujiao; Yang, Hongfen; Liu, Jinquan; Wang, Wenjuan; Chen, Danrong] Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, University of South China, Hengyang, Hunan, 421001, China;[Xie, Hongbin] Hengyang Center for Disease Control and Prevention, Hengyang, Hunan, 421001, China
通讯机构：
[Wang, Wenjuan] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
摘要：
Herein, we presented a paper-based POCT sensor based on lactate dehydrogenase-mediated alginate gelation combined with visual distance reading and smartphone-assisted colorimetric analysis dual-signal to determine the concentrations of L-lactate in yogurt samples. In this research, L-lactate was transformed to pyruvate by lactate dehydrogenase. The pyruvate then triggered the gelation of the sol mixture, increasing the viscosity (ηs) of the mixture, which showed a decrease in diffusion diameter on the paper-based sensor. Also, protons from pyruvate accelerated the degradation of Rhodamine B, causing color fading of the mixture, which could be analyzed by a RGB analysis application software. Under the optimal experimental conditions, the linear ranges of the visual distance reading and smartphone-assisted colorimetric analysis were 0.1-15 μM for the former and 0.3-15 μM for the latter, and the detection limits were 0.03 μM and 0.07 μM, respectively. As a proof-of-concept application, we then exploited the paper-based sensor to determine the concentrations of L-lactate in yogurt samples. The results from the dual-signal paper-based sensor are consistent with the ones from HPLC analysis. In short, this study developed a simple, convenient, cost-effective and feasible method for the quantitative detection of L-lactate in real samples.
作者机构：
[Jing Yang; Shishan Teng; Rongzhang He; Bo Liu; Xingyu Zheng; Rui Lu; Dong Pan; Liting Peng; Wenpei Liu] College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China;Translational Medicine Institute, The First People's Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou, 423000, China;[Zhanpeng Liu; Xiaoyue Zhang; Lulu Liu; Hongying chen] College of Life Sciences, Northwest A&F University, Yangling, 712100, China;[Yi-Ping Li] Institute of Human Virology, Department of Pathogen Biology and Biosecurity, and Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China;[You Wang] School of Public Health, University of South China, Hengyang, 421001, China
通讯机构：
[Hongying chen; Wenpei Liu; Xiaowang Qu] C;[Yi-Ping Li] I;College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China<&wdkj&>Institute of Human Virology, Department of Pathogen Biology and Biosecurity, and Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China<&wdkj&>College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China<&wdkj&>School of Public Health, University of South China, Hengyang, 421001, China<&wdkj&>College of Life Sciences, Northwest A&F University, Yangling, 712100, China
摘要：
Tumor cell metastasis is the key cause of death in patients with nasopharyngeal carcinoma (NPC). MiR-2110 was cloned and identified in Epstein-Barr virus (EBV)-positive NPC, but its role is unclear in NPC. In this study, we investigated the effect of miR-2110 on NPC metastasis and its related molecular basis. In addition, we also explored whether miR-2110 can be regulated by cinobufotalin (CB) and participate in the inhibition of CB on NPC metastasis. Bioinformatics, RT-PCR, and in situ hybridization were used to observe the expression of miR-2110 in NPC tissues and cells. Scratch, Boyden, and tail vein metastasis model of nude mouse were used to detect the effect of miR-2110 on NPC metastasis. Western blot, Co-IP, luciferase activity, colocalization of micro confocal and ubiquitination assays were used to identify the molecular mechanism of miR-2110 affecting NPC metastasis. Finally, miR-2110 induced by CB participates in CB-stimulated inhibition of NPC metastasis was explored. The data showed that increased miR-2110 significantly suppresses NPC cell migration, invasion, and metastasis. Suppressing miR-2110 markedly restored NPC cell migration and invasion. Mechanistically, miR-2110 directly targeted FGFR1 and reduced its protein expression. Decreased FGFR1 attenuated its recruitment of NEDD4, which downregulated NEDD4-induced phosphatase and tensin homolog (PTEN) ubiquitination and degradation and further increased PTEN protein stability, thereby inactivating PI3K/AKT-stimulated epithelial-mesenchymal transition signaling and ultimately suppressing NPC metastasis. Interestingly, CB, a potential new inhibitory drug for NPC metastasis, significantly induced miR-2110 expression by suppressing PI3K/AKT/c-Jun-mediated transcription inhibition. Suppression of miR-2110 significantly restored cell migration and invasion in CB-treated NPC cells. Finally, a clinical sample assay indicated that reduced miR-2110 was negatively correlated with NPC lymph node metastasis and positively related to NPC patient survival prognosis. In summary, miR-2110 is a metastatic suppressor involving in CB-induced suppression of NPC metastasis.
