期刊：
Separation and Purification Technology,2025年354:129241 ISSN：1383-5866
通讯作者：
Zhongran Dai
作者机构：
[Dai, Zhongran; Liang, Beichao; Chen, Lijie] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China;[Zhang, Weilin] College of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China;[Gao, Yuan] School of Chemical Engineering and Technology, China University of Mining & Technology, Xuzhou, Jiangsu 221116, China;[Li, Le] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China<&wdkj&>College of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China
通讯机构：
[Zhongran Dai] K;Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China
摘要：
Photocatalysis holds promise for extracting uranium from aqueous solution. Nevertheless, conventional approaches generally rely on sacrificial agents and anaerobic conditions to maintain photocatalytic efficiency, which increases costs and causes secondary pollution. Herein, we introduce the design and synthesis of an S-scheme ZnIn2S4/g-C3N4 (ZISCN) heterojunction photocatalyst for the efficient removal of uranium via in-situ generating ZnIn2S4 on g-C3N4. Photoelectric characterization and theoretical calculation indicate that ZISCN boosts the absorption of visible light and promotes the effective separation and migration of charge carriers by forming an internal electric field (IEF) at the S-scheme heterojunction interface. This configuration integrates the strong reducing electrons of g-C3N4 and the potent oxidation holes of ZnIn2S4. Consequently, the as-synthesized ZISCN can efficiently remove uranium under an air atmosphere without the need for sacrificial agents and anaerobic conditions. The achieved U(VI) removal rate of 94.8 % surpasses that of ZnIn2S4 and g-C3N4 individually. Moreover, the photocatalytic extraction of U(VI) by ZISCN photocatalyst demonstrated excellent stability and anti-interference performance. After five cycles, the U(VI) removal rate remained above 85 %. Mechanism studies reveal that when electrons are generated by light in the ZISCN systems, they can reduce O2, leading to the formation of reactive species ·O2/H2O2. These species subsequently interact with U(VI), resulting in the precipitation of (UO2)O2·2H2O on the surface of ZISCN. This research provides valuable insights for the design of heterojunction photocatalysts for efficient, sacrificial agent-free uranium removal in ambient air environments.
期刊：
WATER AIR AND SOIL POLLUTION,2025年236(1):1-12 ISSN：0049-6979
通讯作者：
Xue, JH
作者机构：
[Wang, Xinqing; Huang, Boshi; Xue, Jinhua; Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Lin, Dongying] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jingjing; Ren, Lanxing] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Hunan, Peoples R China.
通讯机构：
[Xue, JH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.
关键词：
Cadmium (II);FR;PAN;Fluorescence quenching
摘要：
The objective of this study was to establish a novel method of fluorescence for the determination of cadmium using fluorescein (FR) and 1-(2-Pyridinylazo)-2-Naohthalenol (PAN). In the pH 9.75 buffer solution of the ammonia-ammonium chloride, the energy transfer between FR and the PAN can occur, which can result in the quenching of the fluorescence. The addition of Cd (II) to this system enhances the degree of quenching. This phenomenon has led to the establishment of a novel method for the determination of cadmium, which has been optimized in terms of the required experimental conditions. In the optimal experimental conditions, the fluorescence intensity of the system decreased linearly in the range of 7.81 x 10-7 to 2.14 x 10-5 mol L-1. The linear regression equation used in this study was F = 123.63 + 170.65c (x 10-6 mol L-1) under the correlation coefficient of 0.9951, the detection limit of 2.34 x 10-7 mol L-1, and the relative standard deviation of 0.18%. The proposed method was simple, sensitive, fast, and economical. The method can be employed for the determination of cadmium in real samples (tap-water, pond water and Xiangjiang River) with the results demonstrating consistency with the graphite furnace atomic absorption method (t-test). This approach offers an innovative method for the quantification of Cd (II).
摘要：
Halloysite nanotubes (HNTs) are nanomaterials (NMs) derived from natural clays and have been considered as biocompatible NMs for biomedical uses. However, the cardiovascular toxicity of HNTs has not been thoroughly investigated. In this study, we compared the cardiotoxicity of HNTs and multi-walled carbon nanotubes (MWCNTs), focusing on the changes in Kruppel-like factor (KLF)-mediated signaling pathways. Mice were intravenously injected with 50µg NMs, once a day, for 5 days, and then mouse hearts were removed for experiments. While HNTs or MWCNTs did not induce obvious pathological changes, RNA-sequencing data suggested the alterations of KLF gene expression. We further confirmed an increase of Klf15 positive cells, accompanied by changes in Klf15-related gene ontology (GO) terms. We noticed that most of the changed GO terms are related with the regulation of gene expression, and we confirmed that the NMs increased myoneurin (Mynn) but decreased snail family transcriptional repressor 1 (Snai1), two transcription factors (TFs) related with Klf15. Besides, the changed GO terms also include metal ion binding and positive regulation of glucose import, and we verified an increase of phosphoenolpyruvate carboxykinase 1 (Pck1) and insulin receptor (Insr). However, HNTs and MWCNTs only showed minimal impact on cell death signaling pathways, and no increase in apoptotic sites was observed after NM treatment. We concluded that intravenous administration of HNTs and MWCNTs activated a protective TF, namely Klf15 in mouse aortas, to alter gene expression and signaling pathways related with metal ion binding and glucose import.
摘要：
The concentration of metallic elements is closely associated with overall health. However, the discharge of untreated industrial wastewater can lead to metal -containing pollutants entering the human body through the food chain, disrupting the organism's homeostasis and posing a risk to human health. Covalent organic framework materials (COFs) have emerged as a novel porous material for detecting or adsorbing metal ions due to their unique pore structure, topological structure and flexible design. This paper summarizes the role, toxicity, and sources of metal ions related to human health, as well as the design, synthesis and performance of COFs fluorescent materials for detecting these elements. The interaction mechanism of different fluorescent COFs and metal ions are discussed. Additionally, the remaining challenges and prospects of COFs fluorescence sensors are provided. We believe this review will be useful in directing the development of fluorescent COFs towards metal ions. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
作者：
Gan, Tian;Yu, Jianwei;Deng, Zhongliang;He, Jun
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2024年14:1454076 ISSN：2235-2988
通讯作者：
He, J
作者机构：
[Gan, Tian; He, Jun; He, J] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang, Peoples R China.;[Deng, Zhongliang; Yu, Jianwei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth,Lab Sci, Hengyang, Hunan, Peoples R China.
通讯机构：
[He, J ] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang, Peoples R China.
关键词：
CRISPR/Cas12a;Mycobacterium tuberculosis;enzymatic recombinant isothermal amplification;fluorescence detection;lateral flow test
摘要：
INTRODUCTION: Mycobacterium tuberculosis, the causative agent of human tuberculosis, poses a significant threat to global public health and imposes a considerable burden on the economy. However, existing laboratory diagnostic methods for M. tuberculosis are time-consuming and have limited sensitivity levels. METHODS: The CRISPR/Cas system, commonly known as the "gene scissors", demonstrates remarkable specificity and efficient signal amplification capabilities. Enzymatic recombinase amplification (ERA) was utilized to rapidly amplify trace DNA fragments at a consistent temperature without relying on thermal cyclers. By integrating of CRISPR/Cas12a with ERA, we successfully developed an ERA-CRISPR/Cas12a detection system that enables rapid identification of M. tuberculosis. RESULTS: The sensitivity of the ERA-CRISPR/Cas12a fluorescence and lateral flow systems was 9 copies/μL and 90 copies/μL, respectively. Simultaneously, the detection system exhibited no cross-reactivity with various of respiratory pathogens and non-tuberculosis mycobacteria, demonstrating a specificity of 100%. The positive concordance rate between the ERA-CRISPR/Cas12a fluorescence system and commercial qPCR was 100% in 60 clinical samples. Meanwhile, the lateral flow system showed a positive concordance rate of 93.8% when compared to commercial qPCR. Both methods demonstrated a negative concordance rate of 100%, and the test results can be obtained in 50 min at the earliest. DISCUSSION: The ERA-CRISPR/Cas12a system offers a rapid, sensitive, and specific method that presents a novel approach to laboratory diagnosis of M. tuberculosis.
期刊：
CURRENT ANALYTICAL CHEMISTRY,2024年 ISSN：1573-4110
通讯作者：
Tan, Yan;Xiao, XL
作者机构：
[Chen, Zijie; Tan, Yan; Tan, Y; Xiao, Xilin; Huang, Shaorong; Wu, Qian; Xiao, XL; Liu, Zhen] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Liu, Jingjing] Univ South China, Sch Chem & Chem Engn, Hengyang, Hunan, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Hunan, Peoples R China.
通讯机构：
[Tan, Y; Xiao, XL ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.
关键词：
Magnetic nanoparticles;graphene oxide;adsorption;uranium ions
摘要：
Background The problem of nuclear water pollution is becoming serious worldwide. Uranium, as a metal substance with long half-life radioactivity, is commonly treated by various methods. Adsorption is considered to be one of the most promising methods for treating uranium-containing wastewater.Method Magnetic nanoparticles MnFe2O4 were prepared via the coprecipitation method, followed by modification of silica using the improved St & ouml;ber method. Subsequently, amino was functionalized and grafted onto graphene oxide to prepare a novel magnetic graphene oxide composite MnFe2O4@SiO2-NH2@GO.Results The highest adsorption rate of MnFe2O4@SiO2-NH2@GO for uranium can reach 97.27% in 1 mg<middle dot>L-1 uranium solution, and the adsorption process conformed to the quasi-second-order kinetic model and Langmuir adsorption isotherm model, indicating that it was a monolayer adsorption dominated by chemisorption. The adsorption thermodynamic parameters demonstrated that the adsorption process was a spontaneous endothermic reaction.Conclusion MnFe2O4@SiO2-NH2@GO had excellent adsorption properties for uranium, which has great application potential in the treatment of low-concentration uranium-containing wastewater.
作者机构：
[Liang, Xiao-yu; Li, Zhe; Chen, Wei-peng; Ma, Jun; Liang, XY; Xu, Zhi-min; Ou, Chun-xian; Zhang, Chu-qing; Liu, Zhuo-wei; Luo, Zi-kang] Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Peoples R China.;[Liang, Xiao-yu; Li, Zhe; Chen, Wei-peng; Ma, Jun; Du, Xiao-jing; Xu, Zhi-min; Ou, Chun-xian; Liang, XY; Zhang, Chu-qing; Luo, Zi-kang] Sun Yat Sen Univ, Canc Ctr, Guangdong Key Lab Nasopharyngeal Carcinoma Diag &, Guangzhou, Peoples R China.;[Liang, Xiao-yu; Li, Zhe; Ma, Jun; Du, Xiao-jing; Xu, Zhi-min; Ou, Chun-xian; Liang, XY; Zhang, Chu-qing; Luo, Zi-kang] Sun Yat Sen Univ, Canc Ctr, Dept Radiat Oncol, Guangzhou, Peoples R China.;[Sun, Wan-yang; Wang, Rong; He, Rong-Rong; Sun, WY] Jinan Univ, Guangdong Engn Res Ctr Chinese Med & Dis Susceptib, State Key Lab Bioact Mol & Druggabil Assessment, Guangzhou, Peoples R China.;[Wang, Fudi; Ge, Chao-dong] Zhejiang Univ, Affiliated Hosp 2, Sch Publ Hlth, State Key Lab Expt Hematol,Sch Med, Hangzhou, Peoples R China.
通讯机构：
[Liang, XY ; Ma, J; Liu, ZW ; Liang, XY] S;[Sun, WY ] J;Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Peoples R China.;Sun Yat Sen Univ, Canc Ctr, Guangdong Key Lab Nasopharyngeal Carcinoma Diag &, Guangzhou, Peoples R China.;Sun Yat Sen Univ, Canc Ctr, Dept Radiat Oncol, Guangzhou, Peoples R China.
摘要：
Whether stem-cell-like cancer cells avert ferroptosis to mediate therapy resistance remains unclear. In this study, using a soft fibrin gel culture system, we found that tumor-repopulating cells (TRCs) with stem-cell-like cancer cell characteristics resist chemotherapy and radiotherapy by decreasing ferroptosis sensitivity. Mechanistically, through quantitative mass spectrometry and lipidomic analysis, we determined that mitochondria metabolic kinase PCK2 phosphorylates and activates ACSL4 to drive ferroptosis-associated phospholipid remodeling. TRCs downregulate the PCK2 expression to confer themselves on a structural ferroptosis-resistant state. Notably, in addition to confirming the role of PCK2-pACSL4(T679) in multiple preclinical models, we discovered that higher PCK2 and pACSL4(T679) levels are correlated with better response to chemotherapy and radiotherapy as well as lower distant metastasis in nasopharyngeal carcinoma cohorts. Phosphorylation of ACSL4 by mitochondria-located metabolic kinase PCK2 is critical to regulating ferroptosis-associated phospholipid remodeling in tumor-repopulating cells that are resistant to chemotherapy and radiotherapy.
摘要：
AIM2, a cytosolic innate immune receptor, has the capability to recognize double-stranded DNA (dsDNA). This paper delineates the structural features of AIM2 and its mechanisms of activation, emphasizing its capacity to detect cytosolic DNA and initiate inflammasome assembly. Additionally, we explore the diverse functions of AIM2 in different cells. Insights into AIM2-mediated neuroinflammation provide a foundation for investigating novel therapeutic strategies targeting AIM2 signaling pathways. Furthermore, we present a comprehensive review of the roles of AIM2 in neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD). Finally, we discuss its therapeutic implications. In conclusion, a profound understanding of AIM2 in neurodegenerative diseases may facilitate the development of effective interventions to mitigate neuronal damage and slow disease progression.
摘要：
Microcystin-LR (MC-LR), frequently generated by cyanobacteria, has been demonstrated to raise the likelihood of liver disease. Few previous studies have explored the potential antagonist against MC-LR. Astaxanthin (ASX) has been shown to possess various beneficial effects in regulating lipid metabolism in the liver. However, whether ASX could alleviate MC-LR-induced hepatic lipid metabolic dysregulation is as yet unclear. In this work, the important roles and mechanisms of ASX in countering MC-LR-induced liver damage and lipid metabolic dysregulation were explored for the first time. The findings revealed that ASX not only prevented weight loss but also enhanced liver health after MC-LR exposure. Moreover, ASX effectively decreased triglyceride, total cholesterol, aspartate transaminase, and alanine aminotransferase contents in mice that were elevated by MC-LR. Histological observation showed that ASX significantly alleviated lipid accumulation and inflammation induced by MC-LR. Mechanically, ASX could significantly diminish the expression of genes responsible for lipid generation (Srebp-1c, Fasn, Cd36, Scd1, Dgat1, and Pparg), which probably reduced lipid accumulation induced by MC-LR. Analogously, MC-LR increased intracellular lipid deposition in THLE-3 cells, while ASX decreased these symptoms by down-regulating the expression of key genes in the lipid synthesis pathway. Our results implied that ASX played a crucial part in lipid synthesis and effectively alleviated MC-LR-induced lipid metabolism dysregulation. ASX might be developed as a novel protectant against hepatic impairment and lipid metabolic dysregulation associated with MC-LR. This study offers new insights for further management of MC-LR-related metabolic diseases.
作者机构：
[Tian, Qingzhen; Tang, Zheng; Zhang, Ziyu; Niu, Xiangheng; Li, Shu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Lin, YH; Du, Dan; Niu, Xiangheng; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Zhang, Xiao] Washington State Univ, Sch Chem Engn & Bioengn, Pullman, WA 99164 USA.
通讯机构：
[Lin, YH ; Niu, XH] W;[Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
关键词：
biomarkers;biomedical applications;catalytic signal amplifications;disease diagnosis;nanozymes
摘要：
An overview of nanozyme‐enabled biomedical sensing and diagnosis is presented. The preparation of nanozymes is first summarized, followed by a discussion of typical strategies that are applied to promote the catalytic specificity and activity of nanozymes; whereafter, the main use of nanozymes in biomarker detection and disease diagnosis is discussed; finally, development trends are forecasted, and corresponding challenges are also pointed out. Abstract As nanoscale materials with the function of catalyzing substrates through enzymatic kinetics, nanozymes are regarded as potential alternatives to natural enzymes. Compared to protein‐based enzymes, nanozymes exhibit attractive characteristics of low preparation cost, robust activity, flexible performance adjustment, and versatile functionalization. These advantages endow them with wide use from biochemical sensing and environmental remediation to medical theranostics. Especially in biomedical diagnosis, the feature of catalytic signal amplification provided by nanozymes makes them function as emerging labels for the detection of biomarkers and diseases, with rapid developments observed in recent years. To provide a comprehensive overview of recent progress made in this dynamic field, here an overview of biomedical diagnosis enabled by nanozymes is provided. This review first summarizes the synthesis of nanozyme materials and then discusses the main strategies applied to enhance their catalytic activity and specificity. Subsequently, representative utilization of nanozymes combined with biological elements in disease diagnosis is reviewed, including the detection of biomarkers related to metabolic, cardiovascular, nervous, and digestive diseases as well as cancers. Finally, some development trends in nanozyme‐enabled biomedical diagnosis are highlighted, and corresponding challenges are also pointed out, aiming to inspire future efforts to further advance this promising field.
摘要：
BACKGROUND: Ultrafine particle (UFP) has been linked with higher risks of cardiovascular diseases; however, the biological mechanisms remain to be fully elucidated. OBJECTIVES: This study aims to investigate the cardiovascular responses to short-term UFP exposure and the biological pathways involved. METHODS: A longitudinal panel study was conducted among 32 healthy, non-smoking young adults in Shanghai, China, who were engaged in five rounds of follow-ups between December 2020 and November 2021. Individual exposures were calculated based on the indoor and outdoor real-time measurements. Blood pressure, arterial stiffness, targeted biomarkers, and untargeted proteomics and metabolomics were examined during each follow-up. Linear mixed-effect models were applied to analyze the exposure and health data. The differential proteins and metabolites were used for pathway enrichment analyses. RESULTS: Short-term UFP exposure was associated with significant increases in blood pressure and arterial stiffness. For example, systolic blood pressure increased by 2.10% (95% confidence interval: 0.63%, 3.59%) corresponding to each interquartile increase in UFP concentrations at lag 0-3h, while pulse wave velocity increased by 2.26% (95% confidence interval: 0.52%, 4.04%) at lag 7-12h. In addition, dozens of molecular biomarkers altered significantly. These effects were generally present within 24h after UFP exposure, and were robust to the adjustment of co-pollutants. Molecular changes detected in proteomics and metabolomics analyses were mainly involved in systemic inflammation, oxidative stress, endothelial dysfunction, coagulation, and disturbance in lipid transport and metabolism. DISCUSSION: This study provides novel and compelling evidence on the detrimental subclinical cardiovascular effects in response to short-term UFP exposure. The multi-omics profiling further offers holistic insights into the underlying biological pathways.
