摘要：
An in vivo model is necessary for toxicology. This review analyzed the uses of zebrafish (Danio rerio) in toxicology based on bibliometrics. Totally 56,816 publications about zebrafish from 2002 to 2023 were found in Web of Science Core Collection, with Toxicology as the top 6 among all disciplines. Accordingly, the bibliometric map reveals that "toxicity" has become a hot keyword. It further reveals that the most common exposure types include acute, chronic, and combined exposure. The toxicological effects include behavioral, intestinal, cardiovascular, hepatic, endocrine toxicity, neurotoxicity, immunotoxicity, genotoxicity, and reproductive and transgenerational toxicity. The mechanisms include oxidative stress, inflammation, autophagy, and dysbiosis of gut microbiota. The toxicants commonly evaluated by using zebrafish model include nanomaterials, arsenic, metals, bisphenol, and dioxin. Overall, zebrafish provide a unique and well-accepted model to investigate the toxicological effects and mechanisms. We also discussed the possible ways to address some of the limitations of zebrafish model, such as the combination of human organoids to avoid species differences.
摘要：
Deinococcus radiodurans (DR) exhibits strong resistance to ionizing radiation. In this study, by constructing a radiation-resistant genetically engineered strain overexpressing the Cs gene, the tolerance of the bacterium to aluminum ions was enhanced, thereby achieving the goal of microbial sustainable remediation of uranium-contaminated environments. Methods: 1. Extraction of the recombinant plasmid pRADK-Cs, transformation into DR, and verification. 2. Investigation of factors such as time and initial uranium concentration on the efficiency of uranium accumulation by the recombinant strain, characterized by changes in functional groups and surface morphology before and after accumulation using techniques such as scanning electron microscope. Conclusions: The recombinant strain Deino-Cs can reduce the inhibitory effect of aluminum ions on uranium accumulation capability, and it exhibits a higher uranium accumulation rate compared to the wild-type strain.
期刊：
Journal of Molecular Structure,2024年1298:137042 ISSN：0022-2860
通讯作者：
Wang, T;Mao, Longfei;Li, SQ
作者机构：
[Guo, Yajie; Wang, Tong; Wang, T; Hou, Jingyu] Sun Yat Sen Univ, Affiliated Hosp 8, Dept Emergency, Shenzhen 518033, Peoples R China.;[Wu, Hao] Sun Yat sen Univ, Sun Yat Sen Mem Hosp, Dept Emergency, Guangzhou 510120, Peoples R China.;[Chen, Ying; Liu, Guangnan] Univ South China, Sch Publ Hlth, Hengyang 421000, Peoples R China.;[Chen, Ying; Liu, Guangnan] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China.;[Wang, Dan] Southern Med Univ, Sch Publ Hlth, Guangzhou 510515, Peoples R China.
通讯机构：
[Li, SQ ; Mao, LF] H;[Wang, T ] S;Sun Yat Sen Univ, Affiliated Hosp 8, Dept Emergency, Shenzhen 518033, Peoples R China.;Henan Univ Sci & Technol, Coll Basic Med & Forens Med, Luoyang 471003, Peoples R China.
关键词：
1,2,3-Triazole;Cabotegravir;Anti-cancer
摘要：
A series of 28 novel cabotegravir derivatives, incorporating the 1,2,3-triazole moiety, were designed and synthesized. The synthesized compounds were evaluated for their potential anticancer activity against four different human cancer cell lines: HuH-7 (hepatocellular), MCF-7 (breast), SKOV3 (ovarian), and HCT-116 (colon). Initial biological assessments revealed that several compounds exhibited potent anti-proliferative activity against these cancer cell lines. Notably, compounds KJ9 and KJ23 demonstrated the most pronounced effects, with IC50 values of 6.59 and 7.83 mu M in HuH-7 cells, 27.24 and 8.59 mu M in MCF-7 cells, 4.46 and 6.30 mu M in SKOV3 cells, 23.90 and 17.00 mu M in HCT-116 cells, respectively. Further investigations demonstrated that compound KJ9 and KJ23 induced cell apoptosis, elevated reactive oxygen species (ROS) levels, and ultimately led to cell death. Additionally, western blot analysis revealed altered expressions of proteins involved in autophagy and DNA damage following treatment with compound KJ9 and KJ23. Molecular structure analysis found that the novel compounds tend towards a planar conformation which contained multiple rigid planar structures. This planar structure makes it possible for the compounds to intercalate into DNA and causing DNA damage. These findings suggested that cabotegravir-1,2,3-triazole derivatives possess potential antitumor abilities and warrant further investigations for the development of novel anticancer drugs.
作者：
Kim, Han-Sol;Bui, Quynh Thi Nhu;Shin, Jeongmin;Wang, Hui*;Ki, Jang-Seu
期刊：
Journal of Applied Phycology,2024年:1-15 ISSN：0921-8971
通讯作者：
Wang, Hui;Ki, JS;Wang, H
作者机构：
[Wang, Hui; Kim, Han-Sol; Shin, Jeongmin; Ki, Jang-Seu; Bui, Quynh Thi Nhu; Ki, JS] Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;[Wang, Hui] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, H; Ki, JS ] S;[Wang, H ] U;Sangmyung Univ, Dept Biotechnol, Seoul 03016, South Korea.;Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词：
STX biosynthesis gene (sxt);Promoter;Untranslated region (UTR);Transcriptional regulation;Alexandrium catenella
摘要：
The marine dinoflagellate Alexandrium catenella (Group I) produces saxitoxins (STXs) in marine environments, causing paralytic shellfish poisoning (PSP). The toxins are synthesized by STXs biosynthesis genes (sxt); of them, sxtA and sxtG are considered the most essential. The promoter region and 3'-untranslated region (3'-UTR) are known to regulate gene transcription; however, they have not been sufficiently elucidated in the sxt genes. Herein, we determined and characterized the genomic DNA sequence of the proximal regions of sxtA and sxtG in A. catenella. The promoter regions of sxtA and sxtG in A. catenella were found to contained distinct cis-regulatory elements (CREs), CpG islands, and TTTT-motif (instead of TATA-box), showing a typical eukaryotic promoter. Especially, a number of light-responsive CREs (G-box, SORLIP1AT/2AT, and Sp1) were found, and the gene expression level showed a significant relationship with photosynthesis. MiSeq sequencing of the long PCR amplicons revealed that sxtA was encoded as a single exonic structure that is discrete (> 5 kbp) from other core sxt. In addition, we reported for the first time two types of 3'-UTR in sxtG mRNA, and their lengths and sequences varied among strains and species. Such results suggest that sxt may be regulated by post-transcription. Our results provide insights into the toxin dynamics of toxic dinoflagellates from a molecular perspective.
作者机构：
[Wu, Xiaotian; Song, Zijun; Sun, Shumin; Su, Yunxing; Cheng, Xihao; Yu, Yingying; Yu, Chao; Min, Junxia] The Second Affiliated Hospital, The First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, Cancer Center, Zhejiang University School of Medicine, Hangzhou, China;[Wang, Tianyi] The First Affiliated Hospital, Basic Medical Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany;Division of Nephrology, Department of Medicine, Albert Einstein College of Medicine, Bronx, USA;[Chen, En; Chen, Wenteng; Yu, Yongping] College of Pharmaceutical Science, Zhejiang University, Hangzhou, China
通讯机构：
[Junxia Min; Fudi Wang] T;The Second Affiliated Hospital, The First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, Cancer Center, Zhejiang University School of Medicine, Hangzhou, China<&wdkj&>The First Affiliated Hospital, Basic Medical Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>The Second Affiliated Hospital, The First Affiliated Hospital, School of Public Health, Institute of Translational Medicine, Cancer Center, Zhejiang University School of Medicine, Hangzhou, China
摘要：
Pyroptosis is a gasdermins-mediated programmed cell death that plays an essential role in immune regulation, and its role in autoimmune disease and cancer has been studied extensively. Increasing evidence shows that various microbial infections can lead to pyroptosis, associated with the occurrence and development of microbial infectious diseases. This study reviews the recent advances in pyroptosis in microbial infection, including bacterial, viral, and fungal infections. We also explore potential therapeutic strategies for treating microbial infection-related diseases by targeting pyroptosis.
摘要：
Four new ansamycin derivatives, named 1,19-epithio-geldanamycin A (1), 17-demethoxylherbimycin H (2), herbimycin M (3), and seco-geldanamycin B (4), together with eight known ansamycin analogues (5-12) were isolated from the solid fermentation of marine-derived actinomycete Streptomyces sp. ZYX-F-97. The structures of new compounds were elucidated by extensive spectroscopic analysis as well as nuclear magnetic resonance (NMR) and electronic circular dichroism (ECD) calculations. All the compounds were assayed for their antibacterial activity. Among them, compounds 4, 8, and 12 exhibited remarkable inhibition against Listeria monocytogenes with minimum inhibitory concentrations (MIC) values ranging from 8 mu g & sdot;mL- 1 to 64 mu g & sdot;mL- 1, and displayed moderate inhibition against methicillin-resistant Staphylococcus aureus (MRSA) with MIC value of 64 mu g & sdot;mL- 1. Compounds 4, 8, 9, and 12 showed moderate inhibition activities against both Staphylococcus aureus and Bacillus subtilis with MIC values ranging from 32 mu g & sdot;mL- 1 to 128 mu g & sdot;mL- 1.
摘要：
The discovery of enzyme-like catalytic characteristics in nanomaterials triggers the generation of nanozymes and their multifarious applications. As a class of artificial mimetic enzymes, nanozymes are widely recognized to have better stability and lower cost than natural bio-enzymes, but the lack of catalytic specificity hinders their wider use. To solve the problem, several potential strategies are explored, among which molecular imprinting attracts much attention because of its powerful capacity for creating specific binding cavities as biomimetic receptors. Attractively, introducing molecularly imprinted polymers (MIPs) onto nanozyme surfaces can make animpact on the latter's catalytic activity. As a result, in recent years, MIPs featuring universal fabrication, low cost, and good stability have been intensively integrated with nanozymes for biochemical detection. In this critical review, we first summarize the general fabrication of nanozyme@MIPs, followed by clarifying the potential effects of molecular imprinting on the catalytic performance of nanozymes in terms of selectivity and activity. Typical examples are emphatically discussed to highlight the latest progress of nanozyme@MIPs applied in catalytic analysis. In the end, personal viewpoints on the future directions of nanozyme@MIPs are presented, to provide a reference for studying the interactions between MIPs and nanozymes and attract more efforts to advance this promising area.
摘要：
The N6-methyladenosine (M6A) modification is the most common internal chemical modification of RNA molecules in eukaryotes. This modification can affect mRNA metabolism, regulate RNA transcription, nuclear export, splicing, degradation, and translation, and significantly impact various aspects of physiology and pathobiology. Radiotherapy is the most common method of tumor treatment. Different intrinsic cellular mechanisms affect the response of cells to ionizing radiation (IR) and the effectiveness of cancer radiotherapy. In this review, we summarize and discuss recent advances in understanding the roles and mechanisms of RNA M6A methylation in cellular responses to radiation-induced DNA damage and in determining the outcomes of cancer radiotherapy. Insights into RNA M6A methylation in radiation biology may facilitate the improvement of therapeutic strategies for cancer radiotherapy and radioprotection of normal tissues.
期刊：
Biosensors and Bioelectronics,2024年252:116149 ISSN：0956-5663
通讯作者：
Hongfen Yang<&wdkj&>Ren Cai
作者机构：
[Zhang, Chunxiao; Lyu, Yifan; Wang, Futing; Tan, Weihong] Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China;[Deng, Suping] Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Zhang, Penghui; Jiang, Yifei] The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China;[Yang, Hongfen] Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China. Electronic address: yanghf88@gmail.com;[Xiang, Li] Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Electronic address: xiangli@pku.edu.cn
通讯机构：
[Hongfen Yang] H;[Ren Cai] M;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China<&wdkj&>Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China
关键词：
Double-response 3D DNA nanomachine;High efficiency;Hybridization chain reactions;Laser-scribed graphene;Real-time monitor
摘要：
The microRNA-21 is closely related to chromatin remodeling and epigenetic regulation. In this work, an efficient double-response 3D DNA nanomachine (DRDN) was assembled by co-immobilizing two different lengths of hairpin DNA on the surface of gold nanoparticles (AuNPs) to capture microRNA-21 (miRNA-21), recycle miRNA-21, and trigger hybridization chain reactions (HCR). This work reports the fabrication of a laser-scribed graphene (LSG) electrode with excellent flexibility and electrical conductivity by laser-scribing commercial polyimide films (PI). The as-proposed self-powered biosensing platform presents significantly increased instantaneous current to in real-time monitor miRNA-21 by a capacitor. The biosensing platform exhibited highly sensitive detection of miRNA-21 with a detection limit of 0.142fM in the range of 0.5fM to 1×10(4)fM, and demonstrated high efficiency in the analysis of the tumor markers.
期刊：
International Journal of Biological Macromolecules,2024年259(Pt 1):129104 ISSN：0141-8130
通讯作者：
Liu, Y
作者机构：
[Liu, Yu; Zhang, Shaoqi; Li, Le; Zhen, Deshuai; Yang, Aofeng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;[Liu, Yu; Zhang, Shaoqi; Zhen, Deshuai] Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Grimes, Craig A.] Flux Photon Corp, 5950 Shiloh Rd East, Alpharetta, GA 30005 USA.;[Liu, Yu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Liu, Y ] H;Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
关键词：
Covalent organic framework;Fluorescent probe;In vivo imaging
摘要：
Simple and accurate in vivo monitoring of Fe(3+) is essential for gaining a better understanding of its role in physiological and pathological processes. A novel fluorescent probe was synthesized via in situ solid-state polymerization of 3,4-ethylenedioxythiophene (PEDOT) in the pore channels of a covalent organic framework (COF). The PEDOT@COF fluorescent probe exhibited an absolute quantum yield (QY) 3 times higher than COF. In the presence of Fe(3+) the PEDOT@COF 475nm fluorescence emission, 365nm excitation, is quenched within 180s. Fluorescence quenching is linear with Fe(3+) in the concentration range of 0-960μM, with a detection limit of 0.82μM. The fluorescence quenching mechanism was attributed to inner filter effect (IEF), photoinduced electron transfer (PET) and static quenching (SQE) between PEDOT@COF and Fe(3+). A paper strip-based detector was designed to facilitate practical applicability, and the PEDOT@COF probe successfully applied to fluorescence imaging of Fe(3+) levels in vivo. This work details a tool of great promise for enabling detailed investigations into the role of Fe(3+) in physiological and pathological diseases.
期刊：
Human & Experimental Toxicology,2024年43 ISSN：0960-3271
通讯作者：
Wang, M;Yang, Q
作者机构：
[Ou, Guifang; Liu, Xiaocheng; Qin, Xinru; Liu, Xu; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Nucl Ind Hyg Sch, Clin Res Inst,Hengyang Med Sch, Hengyang, Peoples R China.;[Ou, Guifang; Liu, Xiaocheng; Qin, Xinru; Liu, Xu; Tang, Yonghong; Rang, Ouyan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang, Peoples R China.;[Qin, Xinru; Liu, Xu] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Li, Guojuan] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Endocrinol Dept, Hengyang, Peoples R China.;[Wang, Mu] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, M ; Yang, Q ] U;Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Dept Basic Med,Nucl Ind Hyg Sch, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dongfeng Nan Rd 336, Hengyang, Peoples R China.
摘要：
Human & Experimental Toxicology, Volume 43, Issue , January-December 2024. <br/>Adipose tissue is the main organ that stores lipids and it plays important roles in metabolic balance in the body. We recently reported in Human and Experimental Toxicology that the combined exposure to BPA and fructose may interfere with energy metabolism of adipose tissue. However, it is still unclear whether the combined exposure to BPA and fructose has the possibility to induce lipid remodeling in adipose tissue. In the present study, we performed a widely targeted quantitative lipidomic analysis of the adipose tissue of rats after 6 months of BPA and fructose combined exposure. We totally determined 734 lipid molecules in the adipose tissue of rats. Principal component analysis (PCA) showed the group of the combined exposure to higher-dose (25 μg/kg every other day) BPA and fructose can be distinguished from the groups of control, higher-dose BPA exposure and fructose exposure clearly. Partial least squares-discriminant analysis (PLS-DA) and univariate statistical analysis displayed lipids of PC(18:0_ 20:3), TG(8:0_14:0_16:0), TG(12:0_14:0_16:1), TG(10:0_16:0_16:1), TG(12:0_ 14:0_18:1), TG(14:0_ 16:0_16:1), TG(14:0_14:1_16:1), TG(8:0_ 16:1_16:2), TG(14:1_16:1_ 16:1), TG(16:1_18:1_18:1), TG(16:0_16:1_20:4) and TG(15:0_18:1_ 24:1) may contributed the most to the discrimination. These findings indicated that combined exposure to BPA and fructose has the potential to cause lipid remodeling in adipose tissue.
期刊：
WATER AIR AND SOIL POLLUTION,2024年235(4):1-11 ISSN：0049-6979
通讯作者：
Fei Yang<&wdkj&>Xizi Long
作者机构：
[Yuanyuan Jiang; Yanling Wu; Fei Yang; Xizi Long] The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;[Hongrui Zhang] Central and Southern China Municipal Engineering Design & Research Institute Co., Ltd., Wuhan City, China;[Waheed Miran] School of Chemical and Materials Engineering, National University of Sciences and 13 Technology, Islamabad, Pakistan;State Key Laboratory of Eco-Hydraulics in Northwest Arid Region, Xi’an University of Technology, Xi’an, People’s Republic of China;[Jun Liu] The Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang, China
通讯机构：
[Fei Yang; Xizi Long] T;The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China
摘要：
Peroxymonosulfate (PMS) advanced oxidation is gaining recognition as a promising method for tackling persistent soil pollutants. However, developing an efficient PMS activator remains a formidable task. This study harnessed Shewanella oneidensis MR-1, a model dissimilatory metal-reducing bacterium (DMRB), to synthesize Mn2O3 nanoparticles by oxidizing Mn(II). These nanoparticles were employed to activate PMS for phenanthrene degradation in soil. Remarkably, biogenic Mn2O3 outperformed chemically synthesized Mn2O3, removing 77.4% of phenanthrene compared to 55.7%. This superior performance is attributed to biogenic Mn2O3's faster electron transfer rate and higher Mn(III) ratio, facilitating electron donation to PMS. Additionally, we assessed the feasibility of PMS advanced oxidation for soil remediation by examining microbial community diversity. Given manganese's prevalence in natural soil and groundwater, in-situ biogenic Mn2O3 synthesis emerges as an innovative soil remediation strategy.
摘要：
Microcystins are highly toxic cyanotoxins and have been produced worldwide with the global expansion of harmful cyanobacterial blooms (HABs), posing serious threats to human health and ecosystem safety. Yet little knowledge is available on the underlying process occurring in the aquatic environment with microcystins. Microplastics as vectors for pollutants has received growing attention and are widely found co-existing with microcystins. On the one hand, microplastics could react with microcystins by adsorption, altering their environmental behavior and ecological risks. On the other hand, particular attention should be given to microplastics due to their implications on the outbreak of HABs and the generation and release of microcystins. However, limited reviews have been undertaken to link the co-existing microcystins and microplastics in natural water. This study aims to provide a comprehensive understanding on the environmental relevance of microcystins and microplastics and their potential interactions, with particular emphasis on the adsorption, transport, sources, ecotoxicity and environmental transformation of microcystins affected by microplastics. In addition, current knowledge gaps and future research directions on the microcystins and microplastics are presented. Overall, this review will provide novel insights into the ecological risk of microcystins associated with microplastics in real water environment and lay foundation for the effective management of HABs and microplastic pollution.
通讯机构：
[Yang, SY; Li, L ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Hlth Inspect & Quarantine, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
摘要：
Mercury is a highly toxic element that is widely present in all types of environmental media and can accumulate in living organisms. Prolonged exposure to high levels of mercury can lead to brain damage and death, so the detection of mercury is of great importance. In this study, a cost-effective and easy-to-operate electrochemical sensing method was successfully developed based on an amino-functionalized titanium-based MXene (NH(2)-Ti(3)C(2)T(x)) for the rapid and selective detection of Hg(2+) that could have a coordination effect with the -NH(2) group of NH(2)-Ti(3)C(2)T(x) to promote the efficient accumulation of Hg(2+). In this strategy, the NH(2)-Ti(3)C(2)T(x) was first modified on glassy carbon electrodes (GCE) to fabricate the electrochemical sensor. Benefiting from the excellent electrical conductivity, abundant active sites, and strong adsorption capacity performance of the NH(2)-Ti(3)C(2)T(x), the NH(2)-Ti(3)C(2)T(x) modified GCE (NH(2)-Ti(3)C(2)T(x)/GCE) exhibited satisfactory selectivity and enhanced square wave anodic stripping voltammetry (SWASV) measurement for the rapid detection of trace amounts of Hg(2+) in aqueous solutions. The electrochemical sensor was found to be capable of detecting Hg(2+) with a low detection limit of 8.27 nmol L(-1) and a linear range of 0.5 μmol L(-1) to 50 μmol L(-1). The response time of the electrochemical sensing method was 308 s. In addition, the electrochemical sensing method has good selectivity, repeatability and stability, and multiple heavy metal ions have no effect on its detection, with repeatability and stability RSDs of 1.68% and 1.43%, respectively. Furthermore, the analysis of practical water samples demonstrated that the developed method was highly practical for the actual determination of Hg(2+) with recoveries in the range of 99.22-101.90%.
作者机构：
[Zhang, Shaoqi; Deng, Zhongliang; He, Jun; He, J; Zhen, Deshuai] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Fang, Jing; Zhang, Shaoqi; Deng, Zhongliang; Zhen, Deshuai; Yang, Aofeng; Ma, Qian] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;[Cai, Qingyun; Zhen, Deshuai] Hunan Univ, Sch Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
通讯机构：
[He, J ] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.
关键词：
CRISPR/Cas9;Rolling circle amplification;S. aureus;Silver chain
摘要：
With the rising emphasis on food safety, technology to rapidly identify Staphylococcus aureus (S. aureus) is of great significance. Herein, we developed a novel electrochemical biosensor based on the CRISPR/Cas9 system and rolling circle amplification (RCA)-assisted "silver chain"-linked gold interdigital electrodes (Au-IDE). This sensor utilizes RCA to create DNA long chains that span the Au-IDE, and CRISPR/Cas9 as a recognition component to recognize capture/target dsDNA. Additionally, we used silver staining technology to improve detection sensitivity. Then, we detected S. aureus through impedance changes that occurred when the silver chain between the Au-IDE was connected or broke, with a limit of detection (LOD) of 7CFU/mL and a detection time of 1.5h. Lastly, we successfully employed this sensor to detect S. aureus in real food samples, making it a promising tool for food monitoring.
作者机构：
[Diao, Qiaoqiao; Bu, Zhijian; Tian, Qingzhen; Liu, Jinjin; Tang, Zheng; Liu, Huiqing; Niu, Xiangheng; Li, Shu; Chen, Xinyu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Niu, Xiangheng] Hunan Ecol & Environm Monitoring Ctr, State Environm Protect Key Lab Monitoring Heavy Me, Changsha 410019, Peoples R China.;[Niu, Xiangheng] Qingdao Univ Sci & Technol, Shandong Key Lab Biochem Anal, Qingdao 266042, Peoples R China.;[Niu, Xiangheng] Minjiang Univ, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350108, Peoples R China.
通讯机构：
[Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Hunan Ecol & Environm Monitoring Ctr, State Environm Protect Key Lab Monitoring Heavy Me, Changsha 410019, Peoples R China.;Qingdao Univ Sci & Technol, Shandong Key Lab Biochem Anal, Qingdao 266042, Peoples R China.;Minjiang Univ, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350108, Peoples R China.
摘要：
The increasing environmental pollution and its threats to human health require efficient monitoring and remediation techniques. Enzymes play a significant role in environmental monitoring and remediation, but the vulnerable performance of natural enzymes greatly limits their wider use in complex matrices. As an alternative to natural enzymes, materials with enzyme-like catalytic characteristics have found many environmental applications. With the catalytic signal amplification feature, various nanozyme materials are explored to detect contaminants. Meanwhile, the robust catalytic performance endows nanozymes with potential use in pollutant removal. To highlight the exciting and blooming area, here we made a critical review on nanozyme-involved detection and degradation of environmental pollutants, and the research progress achieved in the last five years was emphatically concluded. First, the use of nanozymes in detecting various contaminants (toxic ions, phenolic pollutants, antibiotics, and pesticides) was discussed according to different sensing principles. Second, the strategies established with the participation of nanozymes for pollutant degradation were summarized. In the end, the opportunities given by nanozyme catalysis were presented for environmental control, and current challenges were discussed to better guide the field's development. Our review provides the up-to-date means and views for monitoring and degrading pollutants based on nanozyme catalysis. It is also anticipated to draw more attention and effort to further advance this promising area. Here we made a critical review on nanozyme-involved detection and degradation of environmental pollutants, and the research progress achieved in the last five years was emphatically concluded.
作者：
Mi Gong;Xiayu Zhou;Xinxu Yang;Craig A. Grimes;Ziwei Tang;...
期刊：
Journal of Radioanalytical and Nuclear Chemistry,2024年:1-12 ISSN：0236-5731
通讯作者：
Le Li<&wdkj&>Deshuai Zhen
作者机构：
[Mi Gong; Xiayu Zhou; Xinxu Yang; Ziwei Tang; Shengyuan Yang; Yue Wang; Lihao Xiong; Jiayi Song; Le Li] Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, College of Public Health, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China;[Craig A. Grimes] Flux Photon Corporation, Alpharetta, USA;[Deshuai Zhen] Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, College of Public Health, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China<&wdkj&>Flux Photon Corporation, Alpharetta, USA
通讯机构：
[Le Li; Deshuai Zhen] H;Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, College of Public Health, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China<&wdkj&>Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, College of Public Health, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China<&wdkj&>Flux Photon Corporation, Alpharetta, USA
摘要：
Presented is an infrared-induced sensor constructed of upconversion nanoparticles and DNAzyme for ultra-sensitive, and rapid detection of uranyl ions. The detection method eliminates the use of chemically labeled nucleic acid probes, possesses high selectivity, and a uranyl detection limit of 39 nM. The technique is successfully applied to the detection of uranyl in various water samples as well as human serum, illustrating the excellent sensitivity and specificity of the quantification platform.
作者机构：
[Gao, Shuaining; Chen, Shi] School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan Province, 421001, P. R. China;[Luo, Jinhua; Huang, Xin; Gao, Shuaining; Guo, Hejiang; Han, Yang; Chen, Shi; Li, Saiyu; Xie, Da-Fei] Department of Radiation Biology, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing, 100850, P. R. China;[Li, Saiyu] School of Life Sciences, Hebei University, Baoding, Hebei Province, 071002, P. R. China;[Luo, Jinhua] Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, Hunan, 410078, P. R. China;[Guan, Hua] School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan Province, 421001, P. R. China. ghlsh@163.com
通讯机构：
[Hua Guan; Ping-Kun Zhou] S;[Ruixue Huang] D;Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, P. R. China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang, P. R. China<&wdkj&>Department of Radiation Biology, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing, P. R. China
摘要：
Metabolic reprogramming, a hallmark of cancer, is closely associated with tumor development and progression. Changes in glycolysis play a crucial role in conferring radiation resistance to tumor cells. How radiation changes the glycolysis status of cancer cells is still unclear. Here we revealed the role of TAB182 in regulating glycolysis and lactate production in cellular response to ionizing radiation. Irradiation can significantly stimulate the production of TAB182 protein, and inhibiting TAB182 increases cellular radiosensitivity. Proteomic analysis indicated that TAB182 influences several vital biological processes, including multiple metabolic pathways. Knockdown of TAB182 results in decreased lactate production and increased pyruvate and ATP levels in cancer cells. Moreover, knocking down TAB182 reverses radiation-induced metabolic changes, such as radioresistant-related lactate production. TAB182 is necessary for activating LDHA transcription by affecting transcription factors SP1 and c-MYC; its knockdown attenuates the upregulation of LDHA by radiation, subsequently suppressing lactate production. Targeted suppression of TAB182 significantly enhances the sensitivity of murine xenograft tumors to radiotherapy. These findings advance our understanding of glycolytic metabolism regulation in response to ionizing radiation, which may offer significant implications for developing new strategies to overcome tumor radioresistance.
