作者机构:
[He, Shuoyao; Li, Jingxian; Lyu, Yifan; Wang, Futing; Yang, Yan; Cai, Ren; Tan, Weihong] Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Coll Biol,Mol Sci& Biomed Lab,State Key Lab Chemo, Changsha 410082, Peoples R China.;[Tan, Weihong] Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp,Hangzhou Inst Med HIM, Hangzhou 310022, Zhejiang, Peoples R China.;[Tan, Weihong] Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200240, Peoples R China.;[Tan, Weihong] Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China.;[Yang, Hongfen; Yang, HF] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构:
[Yang, HF ] U;[Cai, R ] H;Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Coll Biol,Mol Sci& Biomed Lab,State Key Lab Chemo, Changsha 410082, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
摘要:
A novel self-powered biosensor is fabricated for ultrasensitive microRNA-21 (miRNA-21) detection, which includes an enzymatic biofuel cell (EBFC), DNA walkers, a digital multimeter (DMM), and a capacitor. As a novel strategy for signal amplification, DNA walkers are designed in the cathode, while the capacitor stores electrochemical energy from the EBFC to further boost the instantaneous current displayed by the DMM. When miRNA-21 is present, the DNA walkers are provoked to walk from as-opened hairpin structures to other hairpin structures, generating double-strand DNA structures, which stimulate [Ru(NH(3))(6)](3+) to be adsorbed on the cathode surface by electrostatic interaction. Afterward, [Ru(NH(3))(6)](3+) is reduced to [Ru(NH(3))(6)](2+), and the open circuit voltage (E(OCV)) is significantly increased. Depending on the approach of signal amplification from DNA walkers, this biosensor displays an ultrasensitive assay toward miRNA-21 in the range of 0.5 to 10(4) fM, with a detection limit of 0.15 fM. In addition, this self-powered biosensor displays high selectivity for miRNA-21 assay in human serum samples.
通讯机构:
[Yang, HF ] U;[Cai, R ] H;Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Coll Biol,State Key Lab ChemoBio Sensing & Chemome, Changsha 410082, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
摘要:
A self-powered biosensing system with multivariate signal amplification is designed for the ultrasensitive, highly efficient, rapid-response, and real-time detection of platelet-derived growth factor-BB (PDGF-BB). The biosensing system is composed of enzymatic biofuel cells (EBFCs), a capacitor, a digital multimeter (DMM), and a computer. Using the hybridization chain reaction (HCR), a few single DNA chains are transformed into abundant double-helix chains, which stimulates the reduction of [Ru(NH3)(6)](3+) to [Ru(NH3)(6)](2+) by electrostatic interaction, corresponding to the "on" state for HCR. As a result, the open-circuit voltage (E-OCV) is significantly increased in this self-powered biosensing system. When PDGF-BB is present, a binding interaction between the target and the aptamer, i.e., PDGF-BB/Apt, corresponding to the "off" state for HCR, results in a decrease of E-OCV. The PDGF-BB concentration is inversely proportional to E-OCV, allowing readable, effective, and precise real-time detection of PDGF-BB. The detection limit of the biosensing system is 0.031 pg/mL (S/N = 3). This strategy provides a promising and powerful tool for the early clinical diagnosis of related colorectal cancer markers.
作者机构:
[Jing Yang; Shishan Teng; Rongzhang He; Bo Liu; Xingyu Zheng; Rui Lu; Dong Pan; Liting Peng; Wenpei Liu] College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China;Translational Medicine Institute, The First People’s Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou, 423000, China;[Zhanpeng Liu; Xiaoyue Zhang; Lulu Liu; Hongying chen] College of Life Sciences, Northwest A&F University, Yangling, 712100, China;[Yi-Ping Li] Institute of Human Virology, Department of Pathogen Biology and Biosecurity, and Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China;[You Wang] School of Public Health, University of South China, Hengyang, 421001, China
通讯机构:
[Hongying chen; Xiaowang Qu] C;College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China<&wdkj&>School of Public Health, University of South China, Hengyang, 421001, China<&wdkj&>College of Life Sciences, Northwest A&F University, Yangling, 712100, China
期刊:
Journal of Antimicrobial Chemotherapy,2023年78(2):546-549 ISSN:0305-7453
通讯作者:
Jin Zhao
作者机构:
[Zhao, Jin; Zhu, Yue] Shantou Univ, Sch Publ Hlth, Shantou, Peoples R China.;[Jiang, Lijuan; Zheng, Chenli; Liu, Shaochu; Tan, Jingguang; Huang, Yuanmei; Zhao, Jin; Tan, Wei; Xie, Wei; Yang, Zhengrong; Zeng, Guang; Zhu, Yue; Zhang, Yan; Tang, Jie] Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Control & Prevent, Shenzhen, Peoples R China.;[Huang, Yuanmei; Zhao, Jin] Shanxi Med Univ, Sch Publ Hlth, Taiyuan, Peoples R China.;[Zeng, Guang; Tang, Jie] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Wang, Hui; Xu, Liumei; He, Yun; Zhang, Lukun] Third Peoples Hosp Shenzhen, Dept Infect Dis, Shenzhen, Peoples R China.
通讯机构:
[Jin Zhao] S;School of Public Health, Shantou University , Shantou , China<&wdkj&>Department of HIV/AIDS Control and Prevention, Shenzhen Center for Disease Control and Prevention , Shenzhen , China<&wdkj&>School of Public Health, Shanxi Medical University , Taiyuan , China
摘要:
OBJECTIVES: In recent years, integrase strand transfer inhibitor (INSTI)-containing regimens have been increasingly adopted in treatment for HIV/AIDS and promoted as non-occupational post-exposure prophylaxis in China. This study aims to describe the prevalence of resistance to integrase and drug resistance mutations (DRMs) among ART-naive patients in Shenzhen, China. METHODS: Serum samples and demographic information were collected from newly reported ART-naive patients in Shenzhen in 2020. The study sequenced the coding sequence of the HIV-1 integrase gene and determined the DRMs.. RESULTS: In this study, 1682 newly reported cases were included and 1071 of them were successfully sequenced finally. The prevalence of primary drug resistance was 1.77%, with 19 samples showing varying degrees of resistance to INSTIs. The study detected six major DRMs in 16 individuals and eight accessory DRMs in 24 individuals. The prevalence of transmitted drug resistance (TDR) mutations was 1.21%, with five transmitted mutations detected in 13 individuals. The prevalence of drug resistance to raltegravir and elvitegravir was statistically higher than to bictegravir, cabotegravir and dolutegravir. CONCLUSIONS: The prevalence of INSTI resistance in Shenzhen in 2020 was relatively high. Continued surveillance for resistance to INSTIs is recommended and treatment regimens should be adopted based on the pattern of resistance to INSTIs. Dolutegravir or bictegravir is first recommended when considering INSTIs as treatment regimens.
摘要:
Annexin A2 is a Ca(2+) regulated protein belonging to the Annexin family and is found in the cytoplasm and cell membrane. It can exist in a monomeric form or in a heterotetrameric form with the S100A10 dimer. The research on Annexin A2 in tumours is currently active, and studies on its role in pathogen infection are increasing. Annexin A2 plays a crucial role in the life cycle of viruses by mediating adhesion, internalization, uncoating, transport, and release. In the case of parasites, bacteria, mycoplasma, fungi, and other pathogens, Annexin A2 binds to the ligand on the pathogen, which mediates the pathogen's adhesion to the host cell, ultimately leading to infection and damage to the host. Furthermore, some studies have developed biological or chemical drugs that target Annexin A2, which have demonstrated promising anti-infective effects. Thus, targeting Annexin A2 may present a promising therapeutic approach for the treatment of diverse infectious diseases. In summary, this paper provides an overview of Annexin A2 and its role in various pathogens. It highlights its regulation of pathogen infection and its potential as a therapeutic target for the treatment of infectious diseases.
摘要:
The main types of functional nucleic acids, signal amplification elements, and signal amplification technologies for detecting foodborne hazardous substances are described. Chen and coworkers have summarized the advantages and disadvantages of current detection methods based on functional nuclear acids biosensors, and put forward some suggestions for developing more effective detection methods of foodborne hazardous substances, which provide some significant references for the efficient detection of foodborne hazardous substances based on functional nucleic acids in the future. Abstract With the further improvement of food safety requirements, the development of fast, highly sensitive, and portable methods for the determination of foodborne hazardous substances has become a new trend in the food industry. In recent years, biosensors and platforms based on functional nucleic acids, along with a range of signal amplification devices and methods, have been established to enable rapid and sensitive determination of specific substances in samples, opening up a new avenue of analysis and detection. In this paper, functional nucleic acid types including aptamers, deoxyribozymes, and G‐quadruplexes which are commonly used in the detection of food source pollutants are introduced. Signal amplification elements include quantum dots, noble metal nanoparticles, magnetic nanoparticles, DNA walkers, and DNA logic gates. Signal amplification technologies including nucleic acid isothermal amplification, hybridization chain reaction, catalytic hairpin assembly, biological barcodes, and microfluidic system are combined with functional nucleic acids sensors and applied to the detection of many foodborne hazardous substances, such as foodborne pathogens, mycotoxins, residual antibiotics, residual pesticides, industrial pollutants, heavy metals, and allergens. Finally, the potential opportunities and broad prospects of functional nucleic acids biosensors in the field of food analysis are discussed.
通讯机构:
[Liang, GY ] S;Southeast Univ, Minist Educ, Sch Publ Hlth, Key Lab Environm Med Engn, Nanjing, Peoples R China.
关键词:
AOP;Hepatotoxicity;MNPLs;New toxicological methodologies;Organoids and organ-on-chips;“Trojan horse” effect
摘要:
Plastic pollution has become a significant global problem over the years, leading to the continuous decomposition and accumulation of micro/nanoplastics (MNPLs) in the environment. As a result, human exposure to these MNPLs is inevitable. The liver, in particular, is highly susceptible to potential MNPL toxicity. In this study, we systematically reviewed the current literature on MNPLs-induced hepatotoxicity and collected data on toxic events occurring at different biological levels. Then, to better understand the cause-mechanism causality, we developed an Adverse Outcome Pathway (AOP) framework for MNPLs-induced hepatotoxicity. The AOP framework provided insights into the mechanism of MNPL-induced hepatotoxicity and highlighted potential health risks such as liver dysfunction and inflammation, metabolism disorders and liver fibrosis. Moreover, we discussed the potential application of emerging toxicological models in the hepatotoxicity study. Liver organoids and liver-on-chips, which can simulate the structure and function of the liver in vitro, offer a promising alternative platform for toxicity testing and risk assessment. We proposed combining the AOP framework with these emerging toxicological models to improve our understanding of the hepatotoxic effects of MNPLs. Overall, this study performed a preliminary exploration of novel toxicological methodologies to assess the hepatotoxicity of MNPLs, providing a deeper understanding of environmental toxicology.
作者机构:
[Yang, Shengyuan; Tan, Yan; Su, Wenen; Liu, Jinquan; Fan, Pengfei; Li, Qianji] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Wu, Xiwen] Yueyang Cty Ctr Dis Control & Prevent, Yueyang 414100, Hunan, Peoples R China.;[Zhang, Xiaohuan] Yongding Dist Ctr Dis Control & Prevent, Zhangjiajie 427000, Hunan, Peoples R China.
通讯机构:
[Li, Qianji] D;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, People's Republic of China.
作者机构:
[Feng, Jie; Yang, Lichen; Cao, Yang; Shan, Xiaoyun; Lu, Jiaxi; Wang, Lijuan] Chinese Ctr Dis Control & Prevent, Key Lab Trace Element Nutr Natl Hlth Comm, Natl Inst Nutr & Hlth, Beijing 100050, Peoples R China.;[Shan, Xiaoyun] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 241001, Peoples R China.
通讯机构:
[Lichen Yang] K;Key Laboratory of Trace Element Nutrition of National Health Committee, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
关键词:
iron storage biomarkers;type 2 diabetes mellitus;association;women of childbearing age
摘要:
Abstract: High iron stores have been reported to be associated with type 2 diabetes mellitus (T2DM). However, evidence for the associations of iron metabolism with T2DM is inconsistent, and whether there is a threshold effect remains controversial. In the present study, we aimed to examine the associations between various iron biomarkers and the risk of T2DM as well as impaired glucose metabolism (IGM) and hyperglycemia in Chinese women of childbearing age. A total of 1145 women were divided into three groups (normal blood glucose metabolism group; IGM group; T2DM group). Biomarkers of iron metabolism (serum ferritin (SF), transferrin, soluble transferrin receptor (sTfR), transferrin saturation, serum iron, total body iron, and sTfR-to-lgferritin index) were measured. After adjusting for various confounding risk factors, SF and sTfR were positively associated with the risk of IGM (fourth vs. first quartile: SF odds ratio (OR) = 1.93 (95% CI 1.17–3.20) and sTfR OR = 3.08 (95% CI 1.84–5.14)) and T2DM (SF OR = 2.39 (95% CI 1.40–4.06) and sTfR OR = 3.84 (95% CI 2.53–5.83)). There was a nonlinear relationship between SF and risk of T2DM and hyperglycemia (p for nonlinearity < 0.01). Our findings suggested that SF and sTfR could be independent predictors of T2DM risk. Keywords: iron storage biomarkers; type 2 diabetes mellitus; association; women of childbearing age