摘要：
Holmium (Ho) modified TiO2 (Ho-TiO2) nanocomposites have been prepared by a sol-gel process, and Copyright: American Scientific Publishers were used as an effective adsorbent and matrix for the erichment and analysis of bisphenol S and Indigo Delivered by Ingenta by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The low detection limits of bisphenol S (BPS) and Indigo (ID) are 0.1 pg center dot mL-1 and 5 pg center dot mL-1, respectively. Extremely BPS detection could be achieved in tea samples with good recovery (81.5-93.0%).
期刊：
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM,2023年 ISSN：0021-972X
通讯作者：
Zheng, MH;Valenti, L
作者机构：
[Liu, Wen-Yue] Wenzhou Med Univ, Affiliated Hosp 1, Dept Endocrinol, Wenzhou 325000, Peoples R China.;[Liu, Wen-Yue] Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou Key Lab Diabet Res, Wenzhou 325000, Peoples R China.;[Zhang, Ni; Lian, Li-You; Ye, Chen-Hui; Zheng, Ming-Hua; Chen, Wen-Ying] Wenzhou Med Univ, Affiliated Hosp 1, MAFLD Res Ctr, Dept Hepatol, Wenzhou 325000, Peoples R China.;[Zhang, Ni; Lian, Li-You; Ye, Chen-Hui; Zheng, Ming-Hua; Chen, Wen-Ying] Wenzhou Med Univ, Inst Hepatol, Wenzhou 325000, Peoples R China.;[Zhang, Ni; Lian, Li-You; Ye, Chen-Hui; Zheng, Ming-Hua; Chen, Wen-Ying] Key Lab Diag & Treatment Dev Chron Liver Dis, Wenzhou, Zhejiang, Peoples R China.
通讯机构：
[Zheng, MH ] W;[Valenti, L ] U;Wenzhou Med Univ, Affiliated Hosp 1, MAFLD Res Ctr, Dept Hepatol, 2 Fuxue Lane, Wenzhou 325000, Peoples R China.;Univ Milan, Dept Pathophysiol & Transplantat, Via Festa Perdono 7, I-20121 Milan, Italy.
关键词：
Iron overload;Metabolic dysfunction-associated fatty liver disease;Metabolic hyperferritinemia;Metabolic syndrome
摘要：
BACKGROUND: There is limited data on the clinical significance of metabolic hyperferritinemia (MHF) based on the most recent consensus. We aimed to validate the clinical outcomes of MHF in general population and biopsy-proven metabolic dysfunction-associated fatty liver disease (MAFLD) patients. METHODS: NHANES database and PERSONS cohort were included. MHF was defined as elevated serum ferritin with metabolic dysfunction (MD) and stratified into different grades according to ferritin (grade 1: 200 [females]/300 [males] - 550 ng/ml; grade 2: 550 - 1000 ng/ml; grade 3: > 1000 ng/ml). The clinical outcomes, including all-cause death, comorbidities and liver histology were compared between non-MHF and MHF in adjusted models. RESULTS: In NHANES, compared with non-MHF with MD, MHF was related to higher risks of advanced fibrosis (FIB-4, P = 0.036), elevated albumin-creatinine ratio (UACR, P = 0.001) and sarcopenia (P = 0.013). Although the association between all grades of MHF and mortality was insignificant (P = 0.122), grades 2/3 was associated with increased mortality (P = 0.029). While comparing with non-MHF without MD, the harmful effects of MHF were more significant in mortality (P < 0.001), elevated UACR (P < 0.001), cardiovascular disease (P = 0.028), and sarcopenia (P < 0.001). In PERSONS cohort, MHF was associated with more advanced grades of steatosis (P < 0.001), lobular inflammation (P < 0.001), advanced fibrosis (P = 0.017), and more severe hepatocellular iron deposition (P < 0.001). CONCLUSIONS: Both in general population and at-risk individuals with MAFLD, MHF was related with poorer clinical outcomes.
摘要：
Pulmonary fibrosis represents the advanced phase of diverse pulmonary ailments, and at present, a definitive cure for these ailments is lacking. Furthermore, underlying mechanisms causative of these ailments remain elusive. Macrophages are immune cells that resist external stimuli in the early stages after birth. These cells can polarize into the classically (M1) and alternatively (M2) activated macrophages. When stimulated owing to the presence of toxic factors, M1 macrophages produce several pro-inflammatory factors, which mediate the inflammatory injury response of the alveolar tissue. The secretion of diverse growth factors by M2 macrophages contributes to the pathogenesis of aberrant alveolar structural fibrosis and remodeling. The abnormal activity of M2 macrophages is considered a critical factor in the formation of pulmonary fibrosis. In this mini-review, to highlight the clinical implications of research studies, we summarize the role and therapeutic targets of polarized subtypes of macrophages in pulmonary fibrosis and the role of targeting macrophages for the treatment of pulmonary fibrosis. M1-type macrophages and M2-type macrophages can transform into each other.M1-type macrophages participate in the early stage of pulmonary fibrosis by producing inflammatory cytokines, ROS and MMP.M2-type macrophages mediate abnormal alveolar tissue repair and tissue remodeling through the production of TGF-& beta;, FGF, and exosomes.Three therapeutic ideas were summarized in this paper: inhibition of the number and activity of lung macrophages, inhibition of M2-type macrophage polarization, inhibition of TGF-& beta; expression and its signalling pathway.
作者机构：
[Min, Junxia; Yu, Yingying; Wang, Fudi; Cheng, Xihao; Su, Yunxing; Wu, Xiaotian; Cai, Zhaoxian; Song, Zijun; Yu, Chao; Min, JX; Sun, Shumin] Zhejiang Univ, Affiliated Hosp 2, Affiliated Hosp 1, Inst Translat Med,Sch Publ Hlth,Canc Ctr,Sch Med, Hangzhou, Zhejiang, Peoples R China.;[Wang, Fudi; Wang, Tianyi; Cai, Zhaoxian] Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch,Basic Med Sci, Hengyang, Hunan, Peoples R China.;[Chen, Wenteng; Yu, Yongping; Chen, En] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou, Zhejiang, Peoples R China.;[Linkermann, Andreas] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Dept Internal Med 3, Div Nephrol, Dresden, Germany.;[Linkermann, Andreas] Albert Einstein Coll Med, Dept Med, Div Nephrol, Bronx, NY USA.
通讯机构：
[Wang, FD ; Min, JX] Z;Zhejiang Univ, Affiliated Hosp 2, Affiliated Hosp 1, Inst Translat Med,Sch Publ Hlth,Canc Ctr,Sch Med, Hangzhou, Zhejiang, Peoples R China.;Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch,Basic Med Sci, Hengyang, Hunan, Peoples R China.
摘要：
Given the rapidly aging population, aging-related diseases are becoming an excessive burden on the global healthcare system. Metformin has been shown to be beneficial to many age-related disorders, as well as increase lifespan in preclinical animal models. During the aging process, kidney function progressively declines. Currently, whether and how metformin protects the kidney remains unclear. In this study, among longevity drugs, including metformin, nicotinamide, resveratrol, rapamycin, and senolytics, we unexpectedly found that metformin, even at low doses, exacerbated experimentally-induced acute kidney injury (AKI) and increased mortality in mice. By single-cell transcriptomics analysis, we found that death of renal parenchymal cells together with an expansion of neutrophils occurs upon metformin treatment after AKI. We identified programmed cell death by ferroptosis in renal parenchymal cells and blocking ferroptosis, or depleting neutrophils protects against metformin-induced nephrotoxicity. Mechanistically, upon induction of AKI, ferroptosis in renal parenchymal cells initiates the migration of neutrophils to the site of injury via the surface receptor CXCR4-bound to metformin-iron-NGAL complex, which results in NETosis aggravated AKI. Finally, we demonstrated that reducing iron showed protective effects on kidney injury, which supports the notion that iron plays an important role in metformin-triggered AKI. Taken together, these findings delineate a novel mechanism underlying metformin-aggravated nephropathy and highlight the mechanistic relationship between iron, ferroptosis, and NETosis in the resulting AKI.
通讯机构：
[Feng, RL ] F;[Niu, XH ; Pan, JM] J;Jiangsu Univ, Inst Green Chem & Chem Technol, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.
关键词：
Prussian blue analogue;oxidase-like nanozyme;molecular imprinting;tetracycline detection;etching induced activity enhancement
摘要：
As a typical antibiotic pollutant, tetracycline (TC) is producing increasing threats to the ecosystem and human health, and exploring convenient means for monitoring of TC is needed. Here, we proposed alkali-etched imprinted Mn-based Prussian blue analogues featuring superior oxidase-mimetic activity and precise recognition for the colorimetric sensing of TC. Simply etching Mn-based Prussian blue analogues (Mn-PBAs) with NaOH could expose the sites and surfaces to significantly improve their catalytic activity. Density functional theory calculations were employed to screen the molecularly imprinted polymer (MIP) layer for target identification. Consequently, the designed Mn-PBA(NaOH)@MIP possessed the rich channels for substrates to get in touch with the active Mn-PBA(NaOH) core, showing an excellent catalytic capacity to trigger the chromogenic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) without the use of H(2)O(2). If TC was introduced, it would be recognized selectively by the MIP shell and masked the channels for TMB access, resulting in the obstruction of the chromogenic reaction. According to this mechanism, selective optical detection of TC was achieved, and performance stability, reusability, and reliability as well as practicability were also verified, promising potential for TC monitoring in complex matrices. Our work not only presents an effective way to enhance the enzyme-like activity of Prussian blue analogues but also provides a facile approach for TC sensing. Additionally, the work will inspire the exploration of molecularly imprinted nanozymes for various applications.
期刊：
JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE,2023年65(9):725-730 ISSN：1076-2752
通讯作者：
Shi, Y;Zheng, J
作者机构：
[Wen, Xilin; Shi, Yu; Xie, Yuxin; Li, Yichong; Fu, Ziwei] Chinese Acad Med Sci, Natl Clin Res Ctr Cardiovasc Dis, Fuwai Hosp, 12 Langshan Rd, Shenzhen 518057, Guangdong, Peoples R China.;[Xie, Yuxin; Xie, Hongwei; Fu, Ziwei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.;[Zheng, Jing; Zheng, J; Wang, Shuang] Shenzhen Hlth Dev Res & Data Management Ctr, 8 Yuanling Five St, Shenzhen 518106, Guangdong, Peoples R China.
通讯机构：
[Shi, Y ] C;[Zheng, J ] S;Chinese Acad Med Sci, Natl Clin Res Ctr Cardiovasc Dis, Fuwai Hosp, 12 Langshan Rd, Shenzhen 518057, Guangdong, Peoples R China.;Shenzhen Hlth Dev Res & Data Management Ctr, 8 Yuanling Five St, Shenzhen 518106, Guangdong, Peoples R China.
摘要：
Our findings further emphasize the health hazards of solid fuel for cooking and heating. This cohort study provides evidence for the effects of solid fuel use on hypertension among middle-aged and elderly Chinese adults, highlighting the importance of clean energy in reducing the risk of hypertension. ObjectiveThe aim of the study is to assess the association between solid fuel use for cooking or heating and the risk of hypertension among individuals older than 45 years.MethodsBaseline questionnaires were used to collect self-reported primary cooking and heating fuel usage. Outcomes were defined as the time of first diagnosis of hypertension. Data were analyzed using Cox proportional hazards models.ResultsSolid fuel use for cooking was associated with a higher risk of hypertension. The association between solid fuel for cooking and hypertension remained significant among residents in north China, individuals aged 45 to 65 years, urban residents, and nonsmokers. Solid fuel for heating was associated with a higher risk of hypertension only in South China.ConclusionsSolid fuel use may result in an increased risk of hypertension. Our findings further emphasize the health hazards of solid fuel for cooking and heating.
通讯机构：
[Jianming Pan] I;[Lizhang Xu; Xiangheng Niu] S;School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China
通讯机构：
[Yang, HF ] U;[Cai, R ] H;Hunan Univ, Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Coll Mat Sci & Engn,Coll Biol,Mol Sci & Biomed La, Changsha 410082, Hunan, Peoples R China.;Univ South China, Hunan Key Lab Typical Environm Pollut & Hlth Haza, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
摘要：
An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS2 QDs/g-C3N4 as a novel luminophore and CuO/NH2-UiO-66 as a dual-quencher. SnS2 QDs were loaded onto g-C3N4 nanosheets and enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV-vis absorption spectrum of CuO/NH2-UiO-66 exhibits considerable overlap with the ECL emission spectrum of SnS2 QDs/g-C3N4, an important consideration for the RET process. In order to stimulate RET, the ZEN aptamer and complementary DNA are introduced for conjugation between the donor and the acceptor. With the binding interaction between ZEN by its aptamer, CuO/NH2-UiO-66 is removed from the electrode surface, resulting in the inhibition of the RET system and an increase in the ECL signal. Under optimal conditions, the as-prepared aptasensor quantified ZEN from 0.5 mu g<middle dot>mL(-1)to 0.1 fg<middle dot>mL(-1 )with a low limit of detection of 0.085 fg<middle dot>mL(-1), and it exhibited good stability, excellent specificity, high reproducibility, and desirable practicality. The sensing strategy provides a method for mycotoxins assay to monitor food safety
作者机构：
[Liu, Jie; Yang, Shengyuan; Du, Wenfang; Tan, Yan; Li, Hong; Luo, Jie; Feng, Qianqian; Deng, Chenyi; Xiao, Fubing] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci,Hunan Key Lab Typ Environm, Hengyang 421001, Peoples R China.;[Du, Wenfang; Wu, Zhaoyang; Xiao, Fubing] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Du, Wenfang] Binzhou Univ, Coll Biol & Environm Engn, Binzhou 256600, Peoples R China.
通讯机构：
[Fubing Xiao] H;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
作者机构：
[Li, Qian; Xiao, Xilin; Liao, Lifu; Nie, Changming] Univ South China, Sch Chem & Chem Engn, Hunan Prov Key Lab Design & Applicat Actinide Comp, Hengyang 421001, Peoples R China.;[Yang, Jing] Hengyang Market Supervis Inspection & Testing Ctr, Hengyang City 421001, Peoples R China.;[Yu, Wenzhan] Univ South China, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[He, Liqiong; Zhou, Renlong] Univ South China, Sch Publ Hlth, Dept Publ Hlth & Lab Sci, Hengyang 421001, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Peoples R China.
通讯机构：
[Xilin Xiao] S;School of Chemistry and Chemical Engineering, Hunan Province Key Laboratory for the Design and Application of Actinide Complexes, University of South China, Hengyang 421001, P. R. China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, China
作者机构：
[Gao Xiao-yu; Qian Duo; Hu Cong-cong; Yang Sheng-yuan; Liu Zhi-yuan; Liu Bin; Su Wen-en; Yi Yu-xin] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Yang, SY ] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
大豆蛋白金纳米簇;2,6-吡啶二羧酸;生物标志物;炭疽杆菌;荧光探针
摘要：
炭疽杆菌是高致病性病原微生物, 引起的炭疽病在我国属于乙类传染病, 因此建立操作简便、 灵敏准确的炭疽杆菌检测方法对预防和控制炭疽传播, 维护公共卫生安全至关重要。 该研究创新性地提出以绿色材料大豆蛋白为保护剂和还原剂, 采用微波加热法合成了一种具有强烈红色荧光发射的大豆蛋白金纳米簇(SPI-AuNCs)。 采用TEM、 UV-Vis、 FL、 XPS、 FTIR等方法表征了SPI-AuNCs的成功合成和部分特殊性能。 结果表明SPI-AuNCs呈球形, 粒径大小在1.8~3.2 nm范围内, 平均粒径为2.65 nm, 在500～550 nm范围内未出现表面等离子体共振峰; SPI-AuNCs的最大激发波长为370 nm, 最大发射波长为680 nm。 SPI-AuNCs的表面官能团主要有—NH、 —COOH、 —OH、 —SH等官能团, 元素的组成主要包括C、 N、 O、 S、 Au元素。 根据Cu2+与SPI-AuNCs表面基团通过配位作用形成非荧光复合物, 使荧光猝灭; 而2,6-吡啶二羧酸(DPA)与Cu2+具有更高的亲和力, 可将Cu2+从SPI-AuNCs表面竞争下来, 使荧光恢复, 据此建立了一种荧光“关-开”策略检测DPA的新方法。 在最佳实验条件下, 荧光恢复率(ΔF/F1)与DPA的浓度在1.15～70.0 μmol·L-1范围内呈良好的线性关系, 线性方程为ΔF/F1=0.011c+0.131, 相关系数r=0.991, 方法检出限为0.34 μmol·L-1。 同时, 通过加标回收实验研究了湖水和牛奶样品中DPA, 得到加标回收率在97.3%～103.6%, 表明了该方法在环境和食品样本DPA的检测中具有很大的应用潜力, 可以为环境监测和食品安全提供方法学支持。 Bacillus anthracis is a highly pathogenic microorganism. Anthrax is an infectious disease caused by Bacillus anthracis, classified as Class B of our country’s statutory reporting infectious diseases. Therefore, the establishment of simple operation, rapid and sensitive detection methods for Bacillus anthracis is vital for preventing and contralling the spread of anthrax and maintaining public health security. This study innovatively proposed to synthesize soy protein gold nanoclusters (SPI-AuNCs) with strong red fluorescence emission by microwave heating using the green material soy protein as a protective agent and reducing agent.TEM, XPS, FTIR, FL, and UV-Vis characterizations were used to verify successful synthesis of SPI-AuNCs. SPI-AuNCs are spherical, which the particle size is in the range of 1.8～3.2 nm, an average diameter of 2.65 nm, and no surface plasma resonance absorption from 500 to 550 nm. The maximum excitation wavelength of SPI-AuNCs is 370 nm, and the maximum emission wavelength appears at 680 nm. The surface functional groups of SPI-AuNCs mainly include —NH, —COOH, —OH, —SH, and the element composition includes C, N, O, S, Au elements. The fluorescence of SPI-AuNCs could be quenched by the coordination between Cu2+ and surface groups of SPI-AuNCs, while DPA has a stronger chelation effect with Cu2+, which could compete for Cu2+ from the surface of SPI-AuNCs and restore the fluorescence of SPI-AuNCs. Accordingly, a new method for DPA detection based on the fluorescent “off-on” strategy was established. Under the optimal experimental conditions, the fluorescence recovery efficiency (ΔF/F1) performs a good linear relationship with DPA concentration in the range of 1.15~70.0 μmol·L-1. The linear regression equation is ΔF/F1=0.011c+0.131 with high correlation coefficient (r=0.991), and the detection limit is 0.34 μmol·L-1. In addition, the spiking experiments of the DPA in lake water and milk samples were performed. The spiked recoveries were 97.3%~103.6%, indicating that this method has great application potential in DPA detection for environmental and food samples, and can provide methodological guidance for environmental monitoring and food safety.
期刊：
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,2023年78(2):546-549 ISSN：0305-7453
通讯作者：
Jin Zhao
作者机构：
[Zhao, Jin; Zhu, Yue] Shantou Univ, Sch Publ Hlth, Shantou, Peoples R China.;[Jiang, Lijuan; Zheng, Chenli; Liu, Shaochu; Tan, Jingguang; Huang, Yuanmei; Zhao, Jin; Tan, Wei; Xie, Wei; Yang, Zhengrong; Zeng, Guang; Zhu, Yue; Zhang, Yan; Tang, Jie] Shenzhen Ctr Dis Control & Prevent, Dept HIV AIDS Control & Prevent, Shenzhen, Peoples R China.;[Huang, Yuanmei; Zhao, Jin] Shanxi Med Univ, Sch Publ Hlth, Taiyuan, Peoples R China.;[Zeng, Guang; Tang, Jie] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Wang, Hui; Xu, Liumei; He, Yun; Zhang, Lukun] Third Peoples Hosp Shenzhen, Dept Infect Dis, Shenzhen, Peoples R China.
通讯机构：
[Jin Zhao] S;School of Public Health, Shantou University , Shantou , China<&wdkj&>Department of HIV/AIDS Control and Prevention, Shenzhen Center for Disease Control and Prevention , Shenzhen , China<&wdkj&>School of Public Health, Shanxi Medical University , Taiyuan , China
摘要：
OBJECTIVES: In recent years, integrase strand transfer inhibitor (INSTI)-containing regimens have been increasingly adopted in treatment for HIV/AIDS and promoted as non-occupational post-exposure prophylaxis in China. This study aims to describe the prevalence of resistance to integrase and drug resistance mutations (DRMs) among ART-naive patients in Shenzhen, China. METHODS: Serum samples and demographic information were collected from newly reported ART-naive patients in Shenzhen in 2020. The study sequenced the coding sequence of the HIV-1 integrase gene and determined the DRMs.. RESULTS: In this study, 1682 newly reported cases were included and 1071 of them were successfully sequenced finally. The prevalence of primary drug resistance was 1.77%, with 19 samples showing varying degrees of resistance to INSTIs. The study detected six major DRMs in 16 individuals and eight accessory DRMs in 24 individuals. The prevalence of transmitted drug resistance (TDR) mutations was 1.21%, with five transmitted mutations detected in 13 individuals. The prevalence of drug resistance to raltegravir and elvitegravir was statistically higher than to bictegravir, cabotegravir and dolutegravir. CONCLUSIONS: The prevalence of INSTI resistance in Shenzhen in 2020 was relatively high. Continued surveillance for resistance to INSTIs is recommended and treatment regimens should be adopted based on the pattern of resistance to INSTIs. Dolutegravir or bictegravir is first recommended when considering INSTIs as treatment regimens.
