Comparing two magnetic separation pretreatment methods for molecular detection using (q)PCR assays targeting Staphylococcus aureus in milk as models

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成果类型：

期刊论文

作者：

Liao, Xiaoyan;Feng, Bo;Suo, Yujuan;Cui, Yan;Shi, Xianming;...

通讯作者：

Bai, YL

作者机构：

[Qu, Yang; Feng, Bo; Shao, Yi; Suo, Yujuan; Huang, Liujuan; Zhang, Donglai; Bai, Yalong; Liao, Xiaoyan] Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China.

[Liao, Xiaoyan] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.

[Shi, Xianming; Cui, Yan] Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, State Key Lab Microbial Metab, Shanghai, Peoples R China.

[Zhang, Donglai] Hebei Petr Vocat & Tech Univ, Dept Chem Engn, Chengde 067000, Hebei, Peoples R China.

通讯机构：

[Bai, YL ] S

Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China.

语种：

英文

关键词：

Magnetic separation;Staphylococcus aureus;Milk;(q)PCR;Detection

期刊：

LWT

ISSN：

0023-6438

年：

2024

卷：

200

页码：

116167

DOI：

10.1016/j.lwt.2024.116167

基金类别：

Shanghai "Science and Technology Innovation Action Plan" for agriculture project [21N31901100]; Applied Basic Research Project of Shanghai Academy of Agricultural Sciences [202309]; Hebei Natural Science Foundation [C2021411001]; Science Research Project of Hebei Education Department [ZD2021001]

机构署名：

本校为其他机构

院系归属：

公共卫生学院

摘要：

Magnetic separation presents significant potential for culture-independent detection of foodborne pathogens in food samples. In this study, we compared two magnetic separation pretreatment strategies for molecular detection using (q)PCR assays targeting Staphylococcus aureus in milk as models. The first approach employed amino-modified silica magnetic particles (ASMPs) for DNA separation, while the second utilized pig IgG-labeled magnetic beads (IgG-MBs) for cell separation. In the ASMPs-based DNA separation strategy, a sensitivity of 36 CFU/mL...

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Comparing two magnetic separation pretreatment methods for molecular detection using (q)PCR assays targeting Staphylococcus aureus in milk as models

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