Objective:To develop a diagnostic method for the rapid detection of influenza A and B viruses in epidemiological investigation site and primary healthcare institutions.Methods:A method based on LAMP-CRISPR-Cas12b-lateral chromatography was established, which combined isothermal amplification technology with CRISPR-Cas12b system with Lateral Flow Immunoassay technology to detect influenza A and B viruses. Specific LAMP primer sets and crRNAs for influenza A and B viruses were designed. The sensitivity of the method was verified by using the grad...
