摘要：
Three types of carbon dots were synthesized using the same precursor (folic acid and europium nitrate) via different preparation methods (doping and direct coordination). A comprehensive comparison and analysis of the morphology, surface groups, and optical properties of the prepared carbon dots (CD), europium-doped carbon dots (CD-Eu), and europium-functionalized carbon dots (CD@Eu) were conducted. Moreover, due to the higher quantum yield, excellent stability, and outstanding selectivity for UO(2)(2+) exhibited by CD-Eu, we selected CD-Eu as the probe for subsequent applications. CD-Eu showed a sensitive response to UO(2)(2+) within the concentration range 25 ~ 200nM, with a detection limit of 0.84nM (0.42μg·L(-1)). Additionally, CD-Eu demonstrated excellent accuracy and recovery in spiked detection of real water samples. Furthermore, we discovered that this probe could detect UO(2)(2+) both in vitro and in vivo. This strategy provides a promising fluorescent sensor for the detection of UO(2)(2+) in water and biological samples, holding significant implications for addressing UO(2)(2+) contamination issues.
摘要：
With the popularity of health-conscious tea drinking, precise sensing of polyphenols as a main class of antioxidants in tea becomes critical for tea authentication and brewing evaluation. Sensor arrays show great potential for the goal, but currently available sensor arrays always need multiple sensing units and/or multi-dimensional signals, resulting in cumbersome sensor construction and operation as well as data processing. Developing easy-to-fabricate and easy-to-use sensor arrays for efficient discrimination is still challenging. Here we propose a new sensor array that only uses a single signal collected dynamically with oxidase-like MnOOH as a sole sensing material. The synthesized MnOOH nanowires exhibit favorable activity to catalyze the chromogenic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. By taking gallic acid, tannic acid, L-epicatechin, (−)-epigallocatechin, (−)-epicatechin gallate and (−)-epigallocatechin gallate as models, the six tea polyphenols show discrepant inhibitory effects on the above catalytic system. As a result, these polyphenols, no matter as a single component at various concentrations or multi-component mixtures with different ratios, can be well distinguished by the single-nanozyme single-readout sensor array. Besides, different Chinese tea species, black tea varieties and impacts of brewing methods are accurately identified. Evidently, our sensor array avoids the requirement for multiple sensing units and multi-dimensional signals, greatly simplifying the fabrication of sensor arrays and their use, which provides an efficient yet facile tool for tea authentication and brewing evaluation.
With the popularity of health-conscious tea drinking, precise sensing of polyphenols as a main class of antioxidants in tea becomes critical for tea authentication and brewing evaluation. Sensor arrays show great potential for the goal, but currently available sensor arrays always need multiple sensing units and/or multi-dimensional signals, resulting in cumbersome sensor construction and operation as well as data processing. Developing easy-to-fabricate and easy-to-use sensor arrays for efficient discrimination is still challenging. Here we propose a new sensor array that only uses a single signal collected dynamically with oxidase-like MnOOH as a sole sensing material. The synthesized MnOOH nanowires exhibit favorable activity to catalyze the chromogenic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. By taking gallic acid, tannic acid, L-epicatechin, (−)-epigallocatechin, (−)-epicatechin gallate and (−)-epigallocatechin gallate as models, the six tea polyphenols show discrepant inhibitory effects on the above catalytic system. As a result, these polyphenols, no matter as a single component at various concentrations or multi-component mixtures with different ratios, can be well distinguished by the single-nanozyme single-readout sensor array. Besides, different Chinese tea species, black tea varieties and impacts of brewing methods are accurately identified. Evidently, our sensor array avoids the requirement for multiple sensing units and multi-dimensional signals, greatly simplifying the fabrication of sensor arrays and their use, which provides an efficient yet facile tool for tea authentication and brewing evaluation.
期刊：
Journal of Water Process Engineering,2025年71:107416 ISSN：2214-7144
通讯作者：
Wang, H;Wang, Wendong
作者机构：
[Wang, Wendong; Wang, Hui; Xi, Hui; Sun, Yingying] Shaanxi Land Engn Construct Grp Co Ltd, Technol Innovat Ctr Land Engn & Human Settlements, Xian 710049, Peoples R China.;[Wang, Hui] Xian Univ Technol, State Key Lab Water Engn Ecol & Environm Arid Area, Xian 710048, Shaanxi, Peoples R China.;[Liu, Bo; Wang, Hui] Xian Univ Technol, Sch Water Resources & Hydroelect Engn, Dept Municipal & Environm Engn, Xian 710048, Shaanxi, Peoples R China.;[Wang, Wendong; Wang, Hui; Xi, Hui; Sun, Yingying] Xi An Jiao Tong Univ, Sch Human Settlements & Civil Engn, Xian 710049, Peoples R China.;[Li, Xilin] Liaoning Tech Univ, Sch Civil Engn, Fuxin 123000, Liaoning, Peoples R China.
通讯机构：
[Wang, WD] S;[Wang, H ] X;Xian Univ Technol, State Key Lab Water Engn Ecol & Environm Arid Area, Xian 710048, Shaanxi, Peoples R China.;Shaanxi Land Engn Construct Grp Co Ltd, Technol Innovat Ctr Land Engn & Human Settlements, Xian 710049, Peoples R China.;Xi An Jiao Tong Univ, Sch Human Settlements & Civil Engn, Xian 710049, Peoples R China.
关键词：
Extracellular electron transfer;Electroactive microorganisms;Heavy metal removal;Bioelectrochemical system
摘要：
Extracellular electron transfer (EET) plays a crucial role in biogeochemical cycles and energy transformation in microorganisms. Electroactive microorganisms (EAMs) are highly important in environmental pollution remediation, especially in reducing or eliminating extracellular electron acceptors, such as heavy metals with high redox potentials, or producing insoluble metal minerals. There are two mechanisms of microbial EET, such as cytochrome c or conductive cell appendages (nanowires) and electron mediators or shuttles can transfer electrons from microbial cells to outer heavy metal. EPSs, riboflavin, initial concentration of heavy metal ions and pH, and conductive polymers can affect the efficiency of EET and heavy metal reduction. Hence, making full use of conductive minerals, exogenous electron mediators, and biochar to enhance the effectiveness of EET and improve heavy metal removal. Based on the removal mechanisms and strengthening measures, this promising strategy for heavy metal removal has been applied to soil remediation, wastewater treatment, and combined with constructed wetlands. Until now, there are some difficulties to overcome, such as cultivating more EAMs besides Geobacter and Shewanella , improving the tolerance of EAMs through genetic engineering, revealing the complicated EET mechanisms, and applying the strategy to practical implications for environmental remediation, etc.. This review would lead to a renewed awareness of microorganisms' properties and EET in solving energy stagnation and heavy metal pollution treatment problems.
Extracellular electron transfer (EET) plays a crucial role in biogeochemical cycles and energy transformation in microorganisms. Electroactive microorganisms (EAMs) are highly important in environmental pollution remediation, especially in reducing or eliminating extracellular electron acceptors, such as heavy metals with high redox potentials, or producing insoluble metal minerals. There are two mechanisms of microbial EET, such as cytochrome c or conductive cell appendages (nanowires) and electron mediators or shuttles can transfer electrons from microbial cells to outer heavy metal. EPSs, riboflavin, initial concentration of heavy metal ions and pH, and conductive polymers can affect the efficiency of EET and heavy metal reduction. Hence, making full use of conductive minerals, exogenous electron mediators, and biochar to enhance the effectiveness of EET and improve heavy metal removal. Based on the removal mechanisms and strengthening measures, this promising strategy for heavy metal removal has been applied to soil remediation, wastewater treatment, and combined with constructed wetlands. Until now, there are some difficulties to overcome, such as cultivating more EAMs besides Geobacter and Shewanella , improving the tolerance of EAMs through genetic engineering, revealing the complicated EET mechanisms, and applying the strategy to practical implications for environmental remediation, etc.. This review would lead to a renewed awareness of microorganisms' properties and EET in solving energy stagnation and heavy metal pollution treatment problems.
作者机构：
[Zhang, Zhaohui; Wang, Yijin; Tang, Xiaomin; Luo, Sihuan; Cao, Yi] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Changsheng Road No. 28, Zhengxiang District, Hengyang city, Hunan Province, Hengyang 421001, China
摘要：
Recent advances have established lipid droplets as dynamic innate immune hubs coordinating cellular metabolism and defense mechanisms. While previous studies primarily focused on nanomaterials (NMs) altering lipid metabolism to influence lipid droplet dynamics, this study pioneers the investigation of NM-induced immune modulation via Toll-like receptor (TLR) pathways as a novel regulatory axis for lipid droplets. Building on our prior findings that graphene oxide (GO) impaired TLR3-mediated lipid signaling, we systematically explored the role of GO's diameter in modulating this process. Mice were subjected to daily intratracheal instillation of three GO variants (50-200nm, <500nm or > 500nm) at 1mg/kg for 7days. Although no significant change in body weight or organ coefficient was observed, all GO exposure suppressed lipid staining in mouse lungs and livers, correlating with altered co-localization of TLR3 and perilipin 2 (PLIN2), critical regulators of lipid droplet biogenesis. Down-regulation of TLR3 signaling components, namely interferon induced protein with tetratricopeptide repeats 1 (IFIT1), radical S-adenosyl methionine domain containing 2 (RSAD2), and PLIN2, occurred in a diameter-dependent manner, with GO 50-200nm showing the most pronounced effects, likely attributable to the smallest hydrodynamic size and polydispersity index in suspension. This work provides evidence that NM geometry governs TLR-mediated lipid droplet regulation, bridging the knowledge gap between nanotoxicology and immunometabolic cross-talking, a paradigm distinct from conventional lipid metabolism-focused nanotoxicological studies.
摘要：
Background The ongoing threat of the monkeypox virus (MPXV) underscores the need for new antiviral treatments, yet drug targets and candidate therapies are limited.
The ongoing threat of the monkeypox virus (MPXV) underscores the need for new antiviral treatments, yet drug targets and candidate therapies are limited.
Methods Calculating the centrality, conservation, and immunogenicity of MPXV proteins in the network to identify viral drug targets. Constructing the MIP-human protein interaction network and identifying key human proteins as potential drug targets through network topology analysis.
Calculating the centrality, conservation, and immunogenicity of MPXV proteins in the network to identify viral drug targets. Constructing the MIP-human protein interaction network and identifying key human proteins as potential drug targets through network topology analysis.
Results We constructed a comprehensive protein–protein interaction (PPI) network between MPXV and humans, using data from the P-HIPSTer database. This network included 113 viral proteins and 2 607 MPXV-interacting human proteins (MIPs). We identified three MPXV proteins (OPG054, OPG084, and OPG190) as key targets for antiviral drugs, as well as 95 critical MIPs (most interacting MIPs, MMIPs) within the MPXV–human PPI network. Further analysis revealed 31 MMIPs as potential targets for broad-spectrum antiviral agents, supported by their involvement in other viral interactions. Functional enrichment of MIPs indicated their roles in infection and immune-related pathways.
We constructed a comprehensive protein–protein interaction (PPI) network between MPXV and humans, using data from the P-HIPSTer database. This network included 113 viral proteins and 2 607 MPXV-interacting human proteins (MIPs). We identified three MPXV proteins (OPG054, OPG084, and OPG190) as key targets for antiviral drugs, as well as 95 critical MIPs (most interacting MIPs, MMIPs) within the MPXV–human PPI network. Further analysis revealed 31 MMIPs as potential targets for broad-spectrum antiviral agents, supported by their involvement in other viral interactions. Functional enrichment of MIPs indicated their roles in infection and immune-related pathways.
Conclusions In total, we identified 112 drugs targeting MPXV proteins and 371 drugs targeting MMIPs, with fostamatinib, trilostane, and raloxifene being able to inhibit both viral and host proteins. This work provides critical insights into MPXV–human interactions and supports the development of targeted antiviral therapies.
In total, we identified 112 drugs targeting MPXV proteins and 371 drugs targeting MMIPs, with fostamatinib, trilostane, and raloxifene being able to inhibit both viral and host proteins. This work provides critical insights into MPXV–human interactions and supports the development of targeted antiviral therapies.
摘要：
Aim This retrospective study was aimed to evaluate the diagnostic value of a combination of carbohydrate antigen 19–9 (CA19-9), carcinoembryonic antigen (CEA) and protein induced by vitamin K absence or antagonist-Ⅱ (PIVKA-II) in pancreatic cancer.
This retrospective study was aimed to evaluate the diagnostic value of a combination of carbohydrate antigen 19–9 (CA19-9), carcinoembryonic antigen (CEA) and protein induced by vitamin K absence or antagonist-Ⅱ (PIVKA-II) in pancreatic cancer.
Methods Clinical data were collected from 111 pancreatic cancer patients and 158 patients with benign pancreatic diseases (BPD). Serum CA19-9, CEA and PIVKA-II were subjected to receiver operating characteristic curve (ROC) analysis alone and in combination for the diagnosis of pancreatic cancer.
Clinical data were collected from 111 pancreatic cancer patients and 158 patients with benign pancreatic diseases (BPD). Serum CA19-9, CEA and PIVKA-II were subjected to receiver operating characteristic curve (ROC) analysis alone and in combination for the diagnosis of pancreatic cancer.
Results Serum CA19-9, CEA, and PIVKA-II were higher in pancreatic cancer patients than in BPD patients ( P < 0.001). ROC analysis indicated that the cutoff values were 99.390 for CA19-9, 3.065 for CEA, and 42.965 for PIVKA-II, at which the positive rate in pancreatic cancer was 78.38 % for CA19-9, 43.24 % for CEA and 48.65 % for PIVKA-Ⅱ. When serum CA19-9, CEA, and PIVKA-II were used alone, the areas under the curves (AUC), sensitivity and specificity were 0.821, 68.47 % and 89.24 % for CA19-9, 0.763, 61.26 % and 85.44 % for CEA, and 0.681, 45.95 % and 87.34 % for PIVKA-II. When serum CA19-9, CEA, and PIVKA-II were used in combination, the positivity rate was 94.59 % in pancreatic cancer with AUC of 0.903, sensitivity of 81.10 % and specificity of 88.00 %.
Serum CA19-9, CEA, and PIVKA-II were higher in pancreatic cancer patients than in BPD patients ( P < 0.001). ROC analysis indicated that the cutoff values were 99.390 for CA19-9, 3.065 for CEA, and 42.965 for PIVKA-II, at which the positive rate in pancreatic cancer was 78.38 % for CA19-9, 43.24 % for CEA and 48.65 % for PIVKA-Ⅱ. When serum CA19-9, CEA, and PIVKA-II were used alone, the areas under the curves (AUC), sensitivity and specificity were 0.821, 68.47 % and 89.24 % for CA19-9, 0.763, 61.26 % and 85.44 % for CEA, and 0.681, 45.95 % and 87.34 % for PIVKA-II. When serum CA19-9, CEA, and PIVKA-II were used in combination, the positivity rate was 94.59 % in pancreatic cancer with AUC of 0.903, sensitivity of 81.10 % and specificity of 88.00 %.
Conclusion PIVKA-II is a potential serum marker of pancreatic cancer and the combination of CA19-9, CEA, and PIVKA-II is a novel panel of serum markers with promising diagnostic value for pancreatic cancer.
PIVKA-II is a potential serum marker of pancreatic cancer and the combination of CA19-9, CEA, and PIVKA-II is a novel panel of serum markers with promising diagnostic value for pancreatic cancer.
摘要：
Efficient enrichment and recycling of uranyl ion (UO 2 2+ ) from wastewater are crucial for preventing radioactive contamination and protecting human health. Herein, two novel covalent organic frameworks (Tp-PDAN and Tp-PDAN-AO) were synthesized by a simple two-step method. Tp-PDAN-AO exhibits excellent hydrophilicity, thermal stability, and chemical stability, making it suitable for UO 2 2+ removal from acidic wastewater. The high-density amidoxime chelate groups in Tp-PDAN-AO confer high adsorption capacity and selectivity for UO 2 2+ , with a maximum adsorption capacity of 565.30 mg g −1 , reaching equilibrium within 2 h. Additionally, Tp-PDAN-AO exhibits strong photocatalytic activity under UV irradiation, which further enhances U(VI) reduction, boosting both adsorption kinetics and capacity. The maximum adsorption capacity increases to 757.62 mg g −1 with photocatalytic reduction. Density functional theory (DFT) calculations reveal six distinct COF-U(VI) interaction modes, explaining the chelation mechanism between hydrated uranyl ions and Tp-PDAN-AO (ƞ 2 mode), providing insights into the application of COFs with customizable functional groups for UO 2 2+ adsorption.
Efficient enrichment and recycling of uranyl ion (UO 2 2+ ) from wastewater are crucial for preventing radioactive contamination and protecting human health. Herein, two novel covalent organic frameworks (Tp-PDAN and Tp-PDAN-AO) were synthesized by a simple two-step method. Tp-PDAN-AO exhibits excellent hydrophilicity, thermal stability, and chemical stability, making it suitable for UO 2 2+ removal from acidic wastewater. The high-density amidoxime chelate groups in Tp-PDAN-AO confer high adsorption capacity and selectivity for UO 2 2+ , with a maximum adsorption capacity of 565.30 mg g −1 , reaching equilibrium within 2 h. Additionally, Tp-PDAN-AO exhibits strong photocatalytic activity under UV irradiation, which further enhances U(VI) reduction, boosting both adsorption kinetics and capacity. The maximum adsorption capacity increases to 757.62 mg g −1 with photocatalytic reduction. Density functional theory (DFT) calculations reveal six distinct COF-U(VI) interaction modes, explaining the chelation mechanism between hydrated uranyl ions and Tp-PDAN-AO (ƞ 2 mode), providing insights into the application of COFs with customizable functional groups for UO 2 2+ adsorption.
作者机构：
[Qimin Yang; Runqi Su; Rongsheng Wang; Jiexin Chen; Yijun Lin; Tao Tan] Faculty of Applied Sciences, Macao Polytechnic University, Macao, China;[Hanyinghong Su] School of Public Health, University of South China, Hengyang, China;[Tangyi Zeng; Huimei Zhou] The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, China;Key Laboratory of Medical Imaging Precision Theranostics and Radiation Protection, College of Hunan Province, The Affiliated Changsha Central Hospital, University of South China, Changsha, China;Department of Medical Imaging, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, China
通讯机构：
[Zhiyi Chen] S;[Tao Tan] F;School of Public Health, University of South China, Hengyang, China<&wdkj&>The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, China<&wdkj&>Key Laboratory of Medical Imaging Precision Theranostics and Radiation Protection, College of Hunan Province, The Affiliated Changsha Central Hospital, University of South China, Changsha, China<&wdkj&>Department of Medical Imaging, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, China<&wdkj&>Faculty of Applied Sciences, Macao Polytechnic University, Macao, China
关键词：
Medical-large language model;Artificial intelligence (AI);Retrieval-augmented generation (RAG)
摘要：
Recent advancements in large language models (LLMs) have significantly enhanced text generation across various sectors; however, their medical application faces critical challenges regarding both accuracy and real-time responsiveness. To address these dual challenges, we propose a novel two-step retrieval and ranking retrieval-augmented generation (RAG) framework that synergistically combines embedding search with Elasticsearch technology. Built upon a dynamically updated medical knowledge base incorporating expert-reviewed documents from leading healthcare institutions, our hybrid architecture employs ColBERTv2 for context-aware result ranking while maintaining computational efficiency. Experimental results show a 10% improvement in accuracy for complex medical queries compared to standalone LLM and single-search RAG variants, while acknowledging that latency challenges remain in emergency situations requiring sub-second responses in an experimental setting, which can be achieved in real-time using more powerful hardware in real-world deployments. This work establishes a new paradigm for reliable medical AI assistants that successfully balances accuracy and practical deployment considerations.
作者：
Yan Liu;Xinli Song;Jia Wei;Yunfeng Pan;Xizi Long;...
期刊：
Journal of Water Process Engineering,2025年75:107921 ISSN：2214-7144
通讯作者：
Xizi Long<&wdkj&>Fei Yang
作者机构：
[Xizi Long] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;[Xinli Song; Jia Wei; Yunfeng Pan] Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, Hunan, China;Nanshan Center for Chronic Disease Control, Shenzhen, Guangdong, China;[Yan Liu] Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, Hunan, China<&wdkj&>Nanshan Center for Chronic Disease Control, Shenzhen, Guangdong, China;[Fei Yang] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, Hunan, China
通讯机构：
[Xizi Long; Fei Yang] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, Hunan, China<&wdkj&>Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China
摘要：
The global proliferation of cyanobacterial harmful algal blooms (cyanoHABs) poses severe risks to human health and the ecological environment. This study explores the dual functionality of single-chamber microbial fuel cells (MFCs) in generating bioelectricity and degrading microcystins from cyanobacteria. The MFCs achieved a maximum power density of 111 mW/m 2 at a current density of 885 mA/m 2 . Beyond bioenergy production, the MFCs efficiently removed 84.5 % of total chemical oxygen demand (TCOD), 74.5 % of total nitrogen (TN), 85.2 % of protein, and 84.0 % of carbohydrates by the end of the batch run. Notably, the MFCs exhibited significant microcystin removal capabilities, including 79.2 % of microcystin-RR (MC-RR), and complete removal (100 %) of microcystin-LR (MC-LR) and microcystin-YR (MC-YR). High-throughput 16S rRNA gene sequencing revealed a substantial increase in the relative abundance of Sphingopyxis , a bacterium linked to MC biodegradation. Protein-protein interaction (PPI) network analysis highlighted the role of 4-methylaminobutanoate oxidase, and structural modeling suggested that this enzyme, produced by Sphingopyxis , contains a putative ligand-binding pocket for MCs. This molecular docking insight underscores a potential mechanism for MC degradation. These findings demonstrate that simultaneous bioelectricity generation and effective microcystin removal can be achieved in microbial electrochemical systems, offering a promising approach to mitigating the impacts of cyanoHABs.
The global proliferation of cyanobacterial harmful algal blooms (cyanoHABs) poses severe risks to human health and the ecological environment. This study explores the dual functionality of single-chamber microbial fuel cells (MFCs) in generating bioelectricity and degrading microcystins from cyanobacteria. The MFCs achieved a maximum power density of 111 mW/m 2 at a current density of 885 mA/m 2 . Beyond bioenergy production, the MFCs efficiently removed 84.5 % of total chemical oxygen demand (TCOD), 74.5 % of total nitrogen (TN), 85.2 % of protein, and 84.0 % of carbohydrates by the end of the batch run. Notably, the MFCs exhibited significant microcystin removal capabilities, including 79.2 % of microcystin-RR (MC-RR), and complete removal (100 %) of microcystin-LR (MC-LR) and microcystin-YR (MC-YR). High-throughput 16S rRNA gene sequencing revealed a substantial increase in the relative abundance of Sphingopyxis , a bacterium linked to MC biodegradation. Protein-protein interaction (PPI) network analysis highlighted the role of 4-methylaminobutanoate oxidase, and structural modeling suggested that this enzyme, produced by Sphingopyxis , contains a putative ligand-binding pocket for MCs. This molecular docking insight underscores a potential mechanism for MC degradation. These findings demonstrate that simultaneous bioelectricity generation and effective microcystin removal can be achieved in microbial electrochemical systems, offering a promising approach to mitigating the impacts of cyanoHABs.
摘要：
As a novel pollutant, microplastic pollution has become a global environmental concern. Melatonin (MT) has a protective effect on the damage caused by pollutants. However, there is still a lack of research on the transgenerational toxicity of microplastics and the alleviation of microplastics toxicity by MT. In this study, the adult zebrafish was exposed to (0, 0.1 and 1mg/L) polystyrene nanoplastics (PSNP) with or without (1μM) MT for 14days, and embryos (F1) were used for experiments. Our study found that long-term exposure of parents to 1mg/L PSNP reduced fertilization rate and survival rate of offspring, increased the deformity rate and induced embryos to hatch in advance. The growth inhibition of offspring was related to the gene transcription of the growth hormone/insulin-like growth factor axis. Moreover, PSNP caused oxidative stress in offspring, damaged immune system, reduced antioxidant capacity and induced apoptosis. MT supplementation could effectively alleviate the developmental toxicity and oxidative damage of offspring, but the negative effects brought by PSNP could not be completely eliminated. Our research provided a new reference for the protective effect of MT on transgenerational toxicity induced by PSNP.
作者机构：
[Liang, Xiao-yu; Li, Zhe; Chen, Wei-peng; Ma, Jun; Liang, XY; Xu, Zhi-min; Ou, Chun-xian; Zhang, Chu-qing; Liu, Zhuo-wei; Luo, Zi-kang] Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Peoples R China.;[Liang, Xiao-yu; Li, Zhe; Chen, Wei-peng; Ma, Jun; Du, Xiao-jing; Xu, Zhi-min; Ou, Chun-xian; Liang, XY; Zhang, Chu-qing; Luo, Zi-kang] Sun Yat Sen Univ, Canc Ctr, Guangdong Key Lab Nasopharyngeal Carcinoma Diag &, Guangzhou, Peoples R China.;[Liang, Xiao-yu; Li, Zhe; Ma, Jun; Du, Xiao-jing; Xu, Zhi-min; Ou, Chun-xian; Liang, XY; Zhang, Chu-qing; Luo, Zi-kang] Sun Yat Sen Univ, Canc Ctr, Dept Radiat Oncol, Guangzhou, Peoples R China.;[Sun, Wan-yang; Wang, Rong; He, Rong-Rong; Sun, WY] Jinan Univ, Guangdong Engn Res Ctr Chinese Med & Dis Susceptib, State Key Lab Bioact Mol & Druggabil Assessment, Guangzhou, Peoples R China.;[Wang, Fudi; Ge, Chao-dong] Zhejiang Univ, Affiliated Hosp 2, Sch Publ Hlth, State Key Lab Expt Hematol,Sch Med, Hangzhou, Peoples R China.
通讯机构：
[Liang, XY ; Ma, J; Liu, ZW ; Liang, XY] S;[Sun, WY ] J;Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Peoples R China.;Sun Yat Sen Univ, Canc Ctr, Guangdong Key Lab Nasopharyngeal Carcinoma Diag &, Guangzhou, Peoples R China.;Sun Yat Sen Univ, Canc Ctr, Dept Radiat Oncol, Guangzhou, Peoples R China.
摘要：
Whether stem-cell-like cancer cells avert ferroptosis to mediate therapy resistance remains unclear. In this study, using a soft fibrin gel culture system, we found that tumor-repopulating cells (TRCs) with stem-cell-like cancer cell characteristics resist chemotherapy and radiotherapy by decreasing ferroptosis sensitivity. Mechanistically, through quantitative mass spectrometry and lipidomic analysis, we determined that mitochondria metabolic kinase PCK2 phosphorylates and activates ACSL4 to drive ferroptosis-associated phospholipid remodeling. TRCs downregulate the PCK2 expression to confer themselves on a structural ferroptosis-resistant state. Notably, in addition to confirming the role of PCK2-pACSL4(T679) in multiple preclinical models, we discovered that higher PCK2 and pACSL4(T679) levels are correlated with better response to chemotherapy and radiotherapy as well as lower distant metastasis in nasopharyngeal carcinoma cohorts. Phosphorylation of ACSL4 by mitochondria-located metabolic kinase PCK2 is critical to regulating ferroptosis-associated phospholipid remodeling in tumor-repopulating cells that are resistant to chemotherapy and radiotherapy.
摘要：
Obstructive sleep apnea (OSA) is a serious type of sleep disorder that can lead to cardiometabolic and neurocognitive diseases. We utilized smart device-based photoplethysmography technology to collect sleep data from the Chinese population from 2019 to 2022. Distributed lag nonlinear models combined with a generalized nonlinear model or a linear mixed effects model were used to investigate the shortterm associations between daily temperature and indicators of OSA severity. We included a total of 6,232,056 d of sleep monitoring data from 51,842 participants with moderate to severe risk of OSA from 313 Chinese cities. The relationships between ambient temperature and OSA exacerbation, apneahypopnea index (AHI), and minimum oxygen saturation (MinSpO2) were almost linear and present only on the same day. Higher temperatures were associated with a greater risk of OSA exacerbation, with an 8.4% (95% confidence interval (CI): 7.6%-9.3%) increase per 10 degrees C increase in temperature. A 10 degrees C increase in daily temperature corresponded to an AHI increase of 0.70 events/h (95% CI: 0.65-0.76) and a MinSpO2 decrease of 0.18% (95% CI: 0.16%-0.19%). Exposure to elevated temperatures during the night can also lead to adverse effects. The effects of higher temperatures on OSA severity were stronger among men, participants with a body mass index >= 24 kg/m2, those aged 45 years and older, individuals with a history of hypertension and diabetes, and during the cold season. This large-scale, nationwide, longitudinal study provides robust evidence suggesting that higher ambient temperatures may immediately worsen OSA. (c) 2024 Science China Press. Published by Elsevier B.V. and Science China Press. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
作者机构：
[Fu, Lichun; Zhen, Deshuai; Yang, Jing; Xiong, Lihao; Luo, Chunhua] Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;[Zhen, Deshuai; Xiong, Lihao; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Gao, Linxiao; Zhen, Deshuai; Luo, Xiaohu] Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.;[Zhou, Huang] Huaihua Univ, Coll Chem & Mat Engn, Huaihua 418000, Peoples R China.;[Yang, Lixia] Nanchang Hangkong Univ, Key Lab Jiangxi Prov Persistent Pollutants Control, Nanchang 330063, Peoples R China.
通讯机构：
[Gao, LX] Q;[Zhen, DS ] H;Hengyang Market Supervis Inspect & Testing Ctr, Hengyang 421001, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;Qiannan Normal Univ Nationalities, Engn Res Ctr Loss Efficacy & Anticorros Mat Guizho, Sch Chem & Chem Engn, Duyun 558000, Peoples R China.
关键词：
Yb@TiO2;Perfluorooctane Sulfonate;Tetrabromobisphenol A;Contaminants;SELDI-TOF MS
摘要：
Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol-gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg center dot mL-1. The results of the analysis of camellia oil samples show good recovery (80.3%-86.8%) with a low detection limit for TBBPA.
摘要：
Altered gut microbiota and metabolites are important for non-alcoholic fatty liver disease (NAFLD) in children. We aimed to comprehensively examine the effects of gut metabolites on NAFLD progression. We performed integrative metabolomics (untargeted discovery and targeted validation) analysis of non-alcoholic fatty liver (NAFL), non-alcoholic steatohepatitis (NASH), and obesity in children. Fecal samples were collected from 75 subjects in the discovery cohort (25 NAFL, 25 NASH, and 25 obese control children) and 145 subjects in an independent validation cohort (53 NAFL, 39 NASH, and 53 obese control children). Among 2,491 metabolites, untargeted metabolomics revealed a complete NAFLD metabolic map containing 318 increased and 123 decreased metabolites. Then, machine learning selected 65 important metabolites that can distinguish the severity of the NAFLD. Furthermore, precision-targeted metabolomics selected 5 novel gut metabolites from 20 typical metabolites. The functionality of candidate metabolites was validated in hepatocyte cell lines. In the end, this study annotated two novel elevated pathogenic metabolites (dodecanoic acid and creatinine) and a relationship between depleted protective gut microbiota ( Butyricicoccus and Alistipes ), increased inflammation (IL-1β), lipid metabolism (TG), and liver function (ALT and AST). This study demonstrates the role of novel gut metabolites (dodecanoic acid and creatinine), as the fatty acid metabolism regulator contributing to NAFLD development through its influence on inflammation and liver function.
Altered gut microbiota and metabolites are important for non-alcoholic fatty liver disease (NAFLD) in children. We aimed to comprehensively examine the effects of gut metabolites on NAFLD progression. We performed integrative metabolomics (untargeted discovery and targeted validation) analysis of non-alcoholic fatty liver (NAFL), non-alcoholic steatohepatitis (NASH), and obesity in children. Fecal samples were collected from 75 subjects in the discovery cohort (25 NAFL, 25 NASH, and 25 obese control children) and 145 subjects in an independent validation cohort (53 NAFL, 39 NASH, and 53 obese control children). Among 2,491 metabolites, untargeted metabolomics revealed a complete NAFLD metabolic map containing 318 increased and 123 decreased metabolites. Then, machine learning selected 65 important metabolites that can distinguish the severity of the NAFLD. Furthermore, precision-targeted metabolomics selected 5 novel gut metabolites from 20 typical metabolites. The functionality of candidate metabolites was validated in hepatocyte cell lines. In the end, this study annotated two novel elevated pathogenic metabolites (dodecanoic acid and creatinine) and a relationship between depleted protective gut microbiota ( Butyricicoccus and Alistipes ), increased inflammation (IL-1β), lipid metabolism (TG), and liver function (ALT and AST). This study demonstrates the role of novel gut metabolites (dodecanoic acid and creatinine), as the fatty acid metabolism regulator contributing to NAFLD development through its influence on inflammation and liver function.
IMPORTANCE Altered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota ( Butyricicoccus and Alistipes ) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development.
Altered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota ( Butyricicoccus and Alistipes ) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development.
Altered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota ( Butyricicoccus and Alistipes ) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development.
Altered gut microbiota and metabolites are a major cause of non-alcoholic fatty liver disease (NAFLD) in children. This study demonstrated a complete gut metabolic map of children with NAFLD, containing 318 increased and 123 decreased metabolites by untargeted metabolomic. Multiple validation approaches (machine learning and targeted metabolomic) selected five novel gut metabolites for targeted metabolomics, which can distinguish NAFLD status and severity. The gut microbiota ( Butyricicoccus and Alistipes ) and metabolites (creatinine and dodecanoic acid) were novel biomarkers associated with impaired liver function and inflammation and validated by experiments of hepatocyte cell lines. The data provide a better understanding of the importance of gut microbiota and metabolite alterations in NAFLD, which implies that the altered gut microbiota and metabolites may represent a potential target to prevent NAFLD development.
摘要：
A bidirectional self-powered biosensor is constructed for the quasi-simultaneous detection of Pb2+ and Hg2+ based on MoS2@CuS heterostructures as an accelerator and hybridization chain reaction (HCR) as a signal amplification strategy. MoS2@CuS heterostructures significantly facilitate electron transfer between glucose and bioelectrodes, thereby greatly improving the detection signal of self-powered biosensors. This novel biosensor employs the unique sequences of DNAzymes to isolate Pb2+ and Hg2+ by the cleavage effect and thymine (T)–Hg2+–thymine (T) structures, respectively. In the process, Pb2+ cuts the sequence of DNAzyme at the bioanode to trigger glucose oxidation to monitor Pb2+. The as-formed T–Hg2+–T structures activate HCR to reduce [Ru(NH3)6]3+ to detect Hg2+ at the biocathode. It is noteworthy that this biosensor not only realizes Pb2+ or Hg2+ detection in a single-electrode, respectively, but also can quasi-simultaneously detect both Pb2+ and Hg2+ in the bioanode and the biocathode. The novel self-powered biosensor identifies Pb2+ in the range of 106 fM to 10 fM with a limit of detection (LOD) of 3.1 fM and Hg2+ in the range of 106 fM to 1 fM with an LOD of 0.33 fM.
A bidirectional self-powered biosensor is constructed for the quasi-simultaneous detection of Pb2+ and Hg2+ based on MoS2@CuS heterostructures as an accelerator and hybridization chain reaction (HCR) as a signal amplification strategy. MoS2@CuS heterostructures significantly facilitate electron transfer between glucose and bioelectrodes, thereby greatly improving the detection signal of self-powered biosensors. This novel biosensor employs the unique sequences of DNAzymes to isolate Pb2+ and Hg2+ by the cleavage effect and thymine (T)–Hg2+–thymine (T) structures, respectively. In the process, Pb2+ cuts the sequence of DNAzyme at the bioanode to trigger glucose oxidation to monitor Pb2+. The as-formed T–Hg2+–T structures activate HCR to reduce [Ru(NH3)6]3+ to detect Hg2+ at the biocathode. It is noteworthy that this biosensor not only realizes Pb2+ or Hg2+ detection in a single-electrode, respectively, but also can quasi-simultaneously detect both Pb2+ and Hg2+ in the bioanode and the biocathode. The novel self-powered biosensor identifies Pb2+ in the range of 106 fM to 10 fM with a limit of detection (LOD) of 3.1 fM and Hg2+ in the range of 106 fM to 1 fM with an LOD of 0.33 fM.
关键词：
drug resistance;epidemiology;extrapulmonary tuberculosis;prevalence;risk factors
摘要：
OBJECTIVES: This study aimed to investigate the epidemiological and drug resistance (DR) characteristics of extrapulmonary tuberculosis (EPTB) in South-Central China. METHODS: EPTB inpatients who were culture-positive for Mycobacterium tuberculosis were retrospectively included in a study at a provincial TB hospital in Hunan, a province in South-Central China, from January 2013 to December 2021. Demographic, clinical, and drug susceptibility data were retrieved from TB treatment records. Descriptive statistical methods and a Chi-squared test were used to analyze the epidemiological and DR characteristics of EPTB patients. A logistic regression model was used to explore the risk factors of rifampicin-resistant/multidrug-resistant (RR/MDR)-EPTB. RESULTS: A total of 1,324 cases were included. The majority of EPTB patients were in the age range of 20-29 years, were predominantly men (male-to-female ratio: 2.03), and were farmers (65.63%). Most EPTB cases were found in 2013 and 2017 from 2013 to 2021. The most prevalent subtypes of EPTB were lymphatic TB (29.83%, 395/1,324), multiple EPTB (20.85%, 276/1,324), and musculoskeletal TB (14.65%, 194/1,324). Musculoskeletal TB and genitourinary TB predominantly presented as exclusive EPTB forms, while lymphatic TB and pharyngeal/laryngeal TB often co-occurred with pulmonary TB (PTB). Drug susceptibility testing results showed that total DR rates (resistance to any of RFP, isoniazid [INH], streptomycin [STR], and/or ethambutol [EMB]) and RR/MDR rates in EPTB were 25.23% and 12.39%, respectively. Musculoskeletal TB exhibited the highest rates of total DR (31.40%), INH resistance (28.90%), STR resistance (20.10%), EMB resistance (6.20%), MDR (13.90%), and poly-DR (6.70%). The multivariable logistic regression model showed that patients aged from 20 to 59 years (compared to those aged 10 years), workers (compared to retirees), and EPTB patients from the south and west of Hunan (compared to those from the east of Hunan) were at an increased risk of developing RR/MDR EPTB (all OR values > 1). CONCLUSION: Our study provided a detailed account of the epidemiological and DR characteristics of EPTB in Hunan province, China. The significant DR rates, particularly in musculoskeletal TB cases, highlight the need for timely diagnosis, effective drug susceptibility testing, and the development of more effective treatment regimens for EPTB, especially targeting musculoskeletal TB treatments.
摘要：
Iron metabolism plays a crucial role in cell viability, but its relationship with adult stem cells and cancer stem cells is not fully understood. The ferritin complex, responsible for intracellular iron storage, is important in this process. We report that conditional deletion of ferritin heavy chain 1 (Fth1) in the hematopoietic system reduced the number and repopulation capacity of hematopoietic stem cells (HSCs). These effects were associated with a decrease in cellular iron level, leading to impaired mitochondrial function and the initiation of apoptosis. Iron supplementation, antioxidant, and apoptosis inhibitors reversed the reduced cell viability of Fth1-deleted hematopoietic stem and progenitor cells (HSPCs). Importantly, leukemic stem cells (LSCs) derived from MLL-AF9-induced acute myeloid leukemia (AML) mice exhibited reduced Fth1 expression, rendering them more susceptible to apoptosis induced by the iron chelation compared to normal HSPCs. Modulating FTH1 expression using mono-methyl fumarate increased LSCs resistance to iron chelator-induced apoptosis. Additionally, iron supplementation, antioxidant, and apoptosis inhibitors protected LSCs from iron chelator-induced cell death. Fth1 deletion also extended the survival of AML mice. These findings unveil a novel mechanism by which ferritin-mediated iron homeostasis regulates the survival of both HSCs and LSCs, suggesting potential therapeutic strategies for blood cancer with iron dysregulation.
