作者机构：
School of Public Health, Guangdong Medical University, Guangdong, Zhanjiang, 524023, China;School of Public Health, Nanhua University, Hu'nan, Hengyang, 421001, China;[Song; Zou; Yao; Huang; 邹堂斌] 广东医科大学公共卫生学院;[Yang] 南华大学公共卫生学院
摘要：
With growing public concern about the health effects of low-dose radiation, numerous studies have demonstrated that low-dose radiation can cause damage to the immune system, making intervention measures essential. This study investigated the protective effects of silybin against low-dose radiation-induced immune system damage and its underlying mechanisms at both the cellular and animal levels. At the cellular level, CCK-8 assays, ROS measurements, and RT-qPCR analysis revealed that silybin alleviated the reduction in RAW264.7 cell proliferation, intracellular ROS levels, and inflammatory cytokine expression following low-dose radiation exposure. At the animal level, comparative analyses of post-irradiation body weight, peripheral blood cell counts, immune organ coefficients, spleen HE/IHC staining, and spleen immune cell numbers demonstrated that silybin mitigated the radiation-induced decrease in body weight, reduction in peripheral blood leukocyte counts, inflammatory cell infiltration in the spleen, decline in spleen immune cell numbers, and increase in cGAS protein-positive cells. These findings indicate that silybin exerts protective effects against low-dose radiation-induced immune system damage, potentially by regulating the cGAS signaling pathway to reduce radiation-induced cellular injury, thereby enhancing its radioprotective properties.
作者机构：
[Xizi Long; Yuanyuan Jiang; Zhaozhong Zhu; Fei Yang] The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Public Health, University of South China, Hengyang, 421001, China;[Yu Li; Hui Wang] Department of Municipal and Environmental Engineering, Faculty of Water Resources and Hydroelectric Engineering, Xi'an University of Technology, Xi'an, 710048, China;[Nan Hu] Key Discipline Laboratory in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, Hunan, 421001, China;[Junzhan Hong] Hunan Provincial Key Laboratory of Geochemical Processes and Resource Environmental Effects, Geophysical and Geochemical Survey Institute of Hunan, Changsha, Hunan, 410014, China
通讯机构：
[Xizi Long] T;The Key Laboratory of Typical Environmental Pollution and Health Hazards of Hunan Province, School of Public Health, University of South China, Hengyang, 421001, China
关键词：
Uranium bioremediation;Synthetic microbial community;Extracellular electron transfer;eDNA;Pyocyanin
摘要：
Uranium contamination from mining and natural sources poses a major environmental and health risk, as soluble uranium U(VI) readily migrates through groundwater systems. Microbial reduction to insoluble U(IV) via dissimilatory metal-reducing bacteria offers a sustainable remediation method, relying on extracellular electron transfer (EET) to shuttle electrons to extracellular acceptors. Shewanella oneidensis MR-1 ( S .MR-1) serves as a model organism for this process, but its EET efficiency is hindered by limited endogenous redox mediators and biofilm conductivity. Despite advances in genetic engineering, the potential of synthetic microbial communities to enhance EET through interspecies interactions remains underexplored. Here we show a synthetic consortium comprising S .MR-1 and a non-U-reducing isolate, Pseudomonas aeruginosa LXZ1 ( P .LXZ1), that fully reduces U(VI) within 48 h, compared to only 60 % reduction by S .MR-1 alone. This enhancement stems from P .LXZ1-secreted pyocyanin, which binds selectively to S .MR-1's outer-membrane cytochrome OmcA, shifting its redox potential to facilitate directional electron flow along a thermodynamic gradient. Concurrently, conductive extracellular DNA released by P . LXZ1 promotes electron transport and aggregate formation, as evidenced by electrochemical assays, transcriptomics, and molecular dynamics simulations. These synergistic mechanisms alleviate proton-transfer limitations and upregulate metabolic pathways, boosting overall EET rates. By harnessing natural microbial cooperation, this approach provides insights into community-driven metal reduction and paves the way for efficient, scalable bioremediation strategies in contaminated sites.
Uranium contamination from mining and natural sources poses a major environmental and health risk, as soluble uranium U(VI) readily migrates through groundwater systems. Microbial reduction to insoluble U(IV) via dissimilatory metal-reducing bacteria offers a sustainable remediation method, relying on extracellular electron transfer (EET) to shuttle electrons to extracellular acceptors. Shewanella oneidensis MR-1 ( S .MR-1) serves as a model organism for this process, but its EET efficiency is hindered by limited endogenous redox mediators and biofilm conductivity. Despite advances in genetic engineering, the potential of synthetic microbial communities to enhance EET through interspecies interactions remains underexplored. Here we show a synthetic consortium comprising S .MR-1 and a non-U-reducing isolate, Pseudomonas aeruginosa LXZ1 ( P .LXZ1), that fully reduces U(VI) within 48 h, compared to only 60 % reduction by S .MR-1 alone. This enhancement stems from P .LXZ1-secreted pyocyanin, which binds selectively to S .MR-1's outer-membrane cytochrome OmcA, shifting its redox potential to facilitate directional electron flow along a thermodynamic gradient. Concurrently, conductive extracellular DNA released by P . LXZ1 promotes electron transport and aggregate formation, as evidenced by electrochemical assays, transcriptomics, and molecular dynamics simulations. These synergistic mechanisms alleviate proton-transfer limitations and upregulate metabolic pathways, boosting overall EET rates. By harnessing natural microbial cooperation, this approach provides insights into community-driven metal reduction and paves the way for efficient, scalable bioremediation strategies in contaminated sites.
通讯机构：
[Chen, LL ] U;[Bai, YL ] S;Univ South China, Coll Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.;Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China.
关键词：
DNA extraction;DNAdesorption;TiO2 nanoparticles
摘要：
We systematically evaluated the DNA adsorption and desorption efficiencies of several nanoparticles. Among them, titanium dioxide (TiO₂) nanoparticles (NPs), aluminum oxide (Al₂O₃) NPs, and zinc oxide (ZnO) NPs exhibited strong DNA-binding capacities under mild conditions. However, phosphate-mediated DNA displacement efficiencies varied considerably, with only TiO₂ NPs showing consistently superior performance. Further investigation into the DNA adsorption and desorption mechanisms of TiO₂ NPs led to the following key results: (1) TiO₂ NPs achieved over 98% DNA adsorption at room temperature, but efficient desorption required elevated temperatures; (2) phosphate-induced DNA displacement depended on the full exposure of phosphate groups, and short DNA fragments were insufficient to effectively compete with adsorbed DNA; (3) the adsorption mechanism of TiO₂ NPs involved multiple interactions, such as coordination and hydrogen bonding. The combination of strong coordination and weak ionic forces likely contributed to the high efficiency of phosphate-mediated desorption. Under optimized conditions, TiO₂ NPs demonstrated excellent separation efficiency for structurally complex DNA, with recovery rates of 56.92% for genomic DNA and 66.31% for plasmid DNA, notably higher than those of amino-modified silica-coated magnetic nanoparticles (ASMNPs; 38.66% and 33.59%). These results highlight the potential of TiO₂ NPs as a powerful tool for trace DNA isolation under mild, biocompatible conditions, with promising applications in nucleic acid separation and molecular diagnostics.
期刊：
Colloids and Surfaces B: Biointerfaces,2025年254:114797 ISSN：0927-7765
通讯作者：
Xiao, FB
作者机构：
[Yang, Shengyuan; Xiao, Fubing; Wang, Yuxiao; Ding, Ying; Deng, Chenyi; Huang, Sijia; Huang, Haibo] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.;[Chen, Shuangxi] Univ South China, Affiliated Hosp 1, Multiom Res Ctr Brain Disorders, Hengyang Med Sch,Dept Neurol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xiao, FB ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
关键词：
Hydrogel microneedles;MMP-responsive drug delivery;Infected wound healing;Gelatin-curcumin nanoparticles;Inflammation modulation
摘要：
Effective management of bacterial infection and inflammatory responses is critical for accelerating infected wound healing. Herein, we develop a multifunctional hydrogel microneedle system (GMPL@GC MNs) integrating gelatin methacryloyl (GM) as the matrix material, ε-poly-L-lysine (PL) for enhanced antibacterial properties, and gelatin-curcumin nanoparticles (GC NPs) for inflammation modulation. The system exploits elevated matrix metalloproteinase (MMP) levels at wound sites to trigger specific degradation of GC NPs and controlled curcumin (Cur) release. This MMP-responsive mechanism enables reactive oxygen species (ROS) scavenging, inflammatory response suppression, and sustained drug delivery over 96 h. In bacteria-infected wound models, the GMPL@GC MNs demonstrate excellent therapeutic efficacy, achieving 99.1 % wound closure rate within 10 days through combined antibacterial, antioxidant, and anti-inflammatory actions. The system's multifunctionality and microenvironment responsiveness present strong potential for clinical wound management applications.
Effective management of bacterial infection and inflammatory responses is critical for accelerating infected wound healing. Herein, we develop a multifunctional hydrogel microneedle system (GMPL@GC MNs) integrating gelatin methacryloyl (GM) as the matrix material, ε-poly-L-lysine (PL) for enhanced antibacterial properties, and gelatin-curcumin nanoparticles (GC NPs) for inflammation modulation. The system exploits elevated matrix metalloproteinase (MMP) levels at wound sites to trigger specific degradation of GC NPs and controlled curcumin (Cur) release. This MMP-responsive mechanism enables reactive oxygen species (ROS) scavenging, inflammatory response suppression, and sustained drug delivery over 96 h. In bacteria-infected wound models, the GMPL@GC MNs demonstrate excellent therapeutic efficacy, achieving 99.1 % wound closure rate within 10 days through combined antibacterial, antioxidant, and anti-inflammatory actions. The system's multifunctionality and microenvironment responsiveness present strong potential for clinical wound management applications.
期刊：
CELL DEATH & DISEASE,2025年16(1):489 ISSN：2041-4889
通讯作者：
Yang, F
作者机构：
[Zhou, Xiaodie; Tan, Qinmei; Yang, F; Wei, Jia; Yang, Yue; Yang, Fei; Sun, Xiaoya] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang, Hunan, Peoples R China.;[Wei, Jia] Cent South Univ, Xiangya Sch Publ Hlth, Changsha, Hunan, Peoples R China.;[Gao, Shanshan] Beijing Inst Radiat Med, Dept Radiat Biol, Beijing Key Lab Radiobiol, Beijing, Peoples R China.
通讯机构：
[Yang, F ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang, Hunan, Peoples R China.
摘要：
Exposure to MC-LR has been shown to cause multiple organ injury, particularly liver injury, and altered energy metabolism is closely linked. As an effective and efficient way to regulate biological gene expression, N(6)-methyladenosine(m(6)A) modification plays an important role in liver injury caused by microcystin-LR(MC-LR) exposure. For the first time, we reveal the dual mechanism by which AlkB homolog 5(ALKBH5) regulates energy metabolism through an m(6)A-YTHDF3-dependent mechanism. After MC-LR exposure, low levels of ALKBH5 increased the m(6)A modification of Phosphoinositide-3-Kinase Regulatory Subunit 1(PIK3R1) and m(6)A methylation was located at A1557. PIK3R1-m(6)A was recognised by YTH N6-Methyladenosine RNA Binding Protein F3(YTHDF3), which reduced the stability of PIK3R1 RNA, thereby inhibiting PIK3R1 expression and ultimately promoting glycolysis. In concert, low-level ALKBH5 inhibit oxidative phosphorylation by down-regulating the expression of Electron Transfer Flavoprotein Dehydrogenase(ETFDH), Electron Transfer Flavoprotein Subunit Alpha(ETFA) and NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 4(NDUFAF4) through an m(6)A-YTHDF3-dependent mechanism. This dual mechanism has been shown to adversely affect cell survival in MC-LR exposed environments by significantly reducing ATP levels. This study reveals for the first time the signalling pathway and molecular mechanism of MC-LR exposure to liver injury through ALKBH5-mediated m(6)A modification, providing new protective and therapeutic principles.Subject terms: m(6)A modification; Oxidative phosphorylation; Glycolysis The mechanism of m(6)A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR. Created with BioRender.com.
期刊：
Science of The Total Environment,2025年970:179016 ISSN：0048-9697
通讯作者：
Bin Zhou<&wdkj&>Fei Yang
作者机构：
[Gui, Ping-Ping] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;[Zeng, Jia-Yue; Liu, Xiao-Ying; Miao, Yu; Li, Cheng-Ru; Zhu, Jin-Qin; Zhang, Min; Liu, A-Xue; Deng, Yan-Ling; Wu, Yang; Liu, Peng-Hui; Li, Yang-Juan; Zeng, Qiang] Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;[Zeng, Jia-Yue; Liu, Xiao-Ying; Miao, Yu; Li, Cheng-Ru; Zhu, Jin-Qin; Zhang, Min; Liu, A-Xue; Deng, Yan-Ling; Wu, Yang; Liu, Peng-Hui; Li, Yang-Juan] Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;[Deng, Yan-Ling] Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA;[Zhang, Min] Department of Environmental Medicine and Climate Science, Icahn School of Medicine at Mount Sinai, New York, NY, USA
通讯机构：
[Bin Zhou] W;[Fei Yang] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China
关键词：
Dichloroacetic acid;Disinfection byproducts;Liver function parameters;Trichloroacetic acid
摘要：
Background Toxicological studies have documented that disinfection byproducts (DBPs), the ubiquitous drinking water pollutants, induce hepatotoxicity. Yet epidemiological evidence is sparse.
Toxicological studies have documented that disinfection byproducts (DBPs), the ubiquitous drinking water pollutants, induce hepatotoxicity. Yet epidemiological evidence is sparse.
Objective To assess urinary biomarkers of drinking water DBPs in relation to liver function parameters.
To assess urinary biomarkers of drinking water DBPs in relation to liver function parameters.
Methods We included 1204 reproductive-aged women from the Tongji Reproductive and Environmental (TREE) study in Wuhan, China between December 2018 and July 2021. Urinary trichloroacetic acid (TCAA) and dichloroacetic acid (DCAA) as biomarkers of drinking water DBPs were assessed. Serum liver function parameters such as albumin (ALB), total cholesterol (TC), and alkaline phosphatase (ALP) were determined. Urinary DCAA and TCAA concentrations in relation to liver function parameters were examined by multivariate linear regression or restricted cubic spline (RCS) models.
We included 1204 reproductive-aged women from the Tongji Reproductive and Environmental (TREE) study in Wuhan, China between December 2018 and July 2021. Urinary trichloroacetic acid (TCAA) and dichloroacetic acid (DCAA) as biomarkers of drinking water DBPs were assessed. Serum liver function parameters such as albumin (ALB), total cholesterol (TC), and alkaline phosphatase (ALP) were determined. Urinary DCAA and TCAA concentrations in relation to liver function parameters were examined by multivariate linear regression or restricted cubic spline (RCS) models.
Results There was no evidence of urinary TCAA in relation to serum parameters of liver function. However, monotonic dose-response relationships were estimated between elevated tertiles of urinary DCAA concentrations and increased serum ALP (percent change = 4.25 %; 95 % CI: 0.34 %, 8.32 % for the upper vs. lower tertile) and TC levels (percent change = 3.84 %; 95 % CI: 0.63 %, 7.17 % for the upper vs. lower tertile). These associations remained for urinary DCAA modeled as the continuous exposure variable and were linear in the RCS models. Age, body mass index, and passive smoking status did not modify these associations.
There was no evidence of urinary TCAA in relation to serum parameters of liver function. However, monotonic dose-response relationships were estimated between elevated tertiles of urinary DCAA concentrations and increased serum ALP (percent change = 4.25 %; 95 % CI: 0.34 %, 8.32 % for the upper vs. lower tertile) and TC levels (percent change = 3.84 %; 95 % CI: 0.63 %, 7.17 % for the upper vs. lower tertile). These associations remained for urinary DCAA modeled as the continuous exposure variable and were linear in the RCS models. Age, body mass index, and passive smoking status did not modify these associations.
Conclusion DCAA but not TCAA exposure may contribute to damaged liver function in reproductive-aged women.
DCAA but not TCAA exposure may contribute to damaged liver function in reproductive-aged women.
摘要：
PB1-like phages belong to Pbunavirus and are widespread in various environments. This group of phages is a promising candidate for treating human or animal infectious diseases caused by antibiotic-resistant P. aeruginosa. The lipopolysaccharide (LPS) has been identified as the receptor of different PB1-like phages, while little is known about the receptor-binding proteins (RBPs) of these phages. We constructed the tail fiber protein (gp50) of a PB1-like phage, PHW2, and its C- or N-terminus truncation variants to identify its role during the phage infection. The anti-gp50((453-964)) antibody showed a similar effect to the antibody against gp50 in blocking the phage infection. The protein competition and cell binding assays showed that the gp50((1-451)) doesn't exhibit an effect on the adsorption of the host cells. These results indicated that the C-terminus of gp50 is the essential region that mediates phage PHW2 adsorption and infection.
作者机构：
[Li, Yuanxiu; Shi, Sicheng; Liu, Qijing; "Li, Feng; Yu, Huan; Wu, Shengbo; An, Xingjuan; Zhang, Baocai; Li, Chao; You, Zixuan; Tang, Rui] State Key Laboratory of Synthetic Biology, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China;[Yu, Huan; Zhang, Baocai] College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China;[Long, Xizi] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China;[Shi, Liang] Department of Biological Sciences and Technology, School of Environmental Studies, China University of Geoscience in Wuhan, Wuhan, Hubei, 430074, China;[Nealson, Kenneth H.] Departments of Earth Science & Biological Sciences, University of Southern California, 4953 Harriman Ave., South Pasadena, CA, 91030, USA
通讯机构：
[Hao Song] S;State Key Laboratory of Synthetic Biology, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, China<&wdkj&>College of Life and Health Sciences, Northeastern University, Shenyang, China
摘要：
Electron shuttle plays a decisive role in extracellular electron transfer (EET) of exoelectrogens. However, neither identifying the most efficient electron shuttle molecule nor programming its optimal synthesis level that boosts EET has been established. Here, the phenazine-1-carboxylic acid (PCA) biosynthesis pathway is first constructed to synthesize PCA at an optimal level for EET in Shewanella oneidensis MR-1. To facilitate PCA transport, the porin OprF is expressed to improve cell membrane permeability, the cytotoxicity of which, however, impaired cell growth. To mitigate cytotoxicity, PCA biosensor is designed to dynamically decouple PCA biosynthesis and transport, resulting in the maximum output power density reaching 2.85 ± 0.10 W m−2, 33.75-fold higher than wild-type strain. Moreover, extensive analyses of cellular electrophysiology, metabolism, and behaviors reveal PCA shuttles electrons from cell to electrode, which is the dominant mechanism underlying PCA-boosted EET. We conclude dynamic synthesis and transport of PCA is an efficient strategy for enhancing EET. Electron shuttle-mediated extracellular electron transfer (EET) is most critical in determining the efficiency of chemical-to-power of exoelectrogens. Here, the authors show that addition of phenazine-1-carboxylic acid at ~80 µM leads to the improved power generation in Shewenella oneidensis MR-1.
摘要：
BACKGROUND: Esophageal cancer ranks as the 11th most diagnosed cancer worldwide and the 7th leading cause of cancer-related deaths, mainly due to late-stage diagnosis. Identifying novel biomarkers is essential for enhancing prognostic evaluations and targeting patients for immunotherapy. METHODS: We used the DepMap database to identify survival-dependent genes in esophageal carcinoma cells. A prognostic model was developed using univariate and multivariate Cox regression and LASSO, validated with the GEO dataset. WGCNA and GSEA analyses were conducted to explore mechanisms, alongside ESTIMATE and ssGSEA for prognosis. RESULTS: We constructed a novel four-gene prognostic signature (CPSF6, IGBP1, MTG2, TCP1) based on SDG expression and survival data. This signature stratified esophageal cancer patients into high- and low-risk groups with significantly different survival, with the high-risk group showing shorter survival. WGCNA and GSEA analyses linked prognosis to immune pathways, including interferon-γ response and IL6-JAK-STAT3 signaling. ssGSEA revealed reduced infiltration of 19 immune cell types in high-risk patients, and ESTIMATE analysis confirmed the association between immune infiltration and poor prognosis. CONCLUSION: This study establishes a four-gene survival signature for esophageal cancer that distinguishes high-risk from low-risk populations, providing novel prognostic indicators. Immune response pathways were downregulated in high-risk patients, offering potential targets for understanding esophageal cancer mechanisms and developing immunotherapeutic strategies.
作者机构：
[Lei Zhang; Kaimin Chen; Luchang Li; Xiu Wei; Xianxin Cai; Yaohui Yu; Yixian Yang; Renping Cao; Fenghua Wei; Bang Lan; Xiaohu Luo; Hui Liang] Northeast Guangdong Key Laboratory of New Functional Materials, Guangdong Rare Earth Photofunctional Materials Engineering Technology Research Center, Meizhou Rare Earth Photo functional Materials Engineering Technology Research Center, Office of Assets&Laboratory Management, School of Chemistry and Environment, Jiaying University, Meizhou, 514015, China;[Deshuai Zhen] Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Yanhua Li] School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang, 421001, China
通讯机构：
[Yanhua Li] S;[Hui Liang] N;[Deshuai Zhen] H;Northeast Guangdong Key Laboratory of New Functional Materials, Guangdong Rare Earth Photofunctional Materials Engineering Technology Research Center, Meizhou Rare Earth Photo functional Materials Engineering Technology Research Center, Office of Assets&Laboratory Management, School of Chemistry and Environment, Jiaying University, Meizhou, 514015, China<&wdkj&>School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang, 421001, China<&wdkj&>Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China
摘要：
Developing non-precious electrocatalysts with high-efficiency hydrogen evolution reaction (HER) activity for hydrogen production at all pH values is crucial for the application and promotion of water electrolysis. Herein, a unique structure of nickel-cobalt (Ni-Co) nanoparticles@Ni 0.19 Co 0.26 P nanowires core/shell arrays on Ni foam as a pH-universal electrocatalyst for hydrogen evolution is synthesized by a simple hydrothermal process and thermal reduction treatment. The morphological analysis reveals that numerous Ni-Co nanoparticles are densely packed inside the hollow interior of Ni 0.19 Co 0.26 P nanowires. This unique structure integrates the advantages of high surface area from the nanowires and enhanced electronic conductivity from the Ni-Co nanoparticles, effectively addressing the conventional issue of slow charge transfer rate in 3D metal-based compounds, which is typically caused by a long transfer distance. The electrocatalysts provide an excellent hydrogen evolution reaction performance in alkaline, neutral, and acidic media with an overpotential of 35, 36, and 23 mV to reach the current density of 10 mA/cm 2 , respectively. This study highlights the potential of Ni-Co nanoparticles@Ni 0.19 Co 0.26 P nanowires as a cost-effective and pH-universal HER electrocatalyst, offering promising prospects for sustainable hydrogen production.
Developing non-precious electrocatalysts with high-efficiency hydrogen evolution reaction (HER) activity for hydrogen production at all pH values is crucial for the application and promotion of water electrolysis. Herein, a unique structure of nickel-cobalt (Ni-Co) nanoparticles@Ni 0.19 Co 0.26 P nanowires core/shell arrays on Ni foam as a pH-universal electrocatalyst for hydrogen evolution is synthesized by a simple hydrothermal process and thermal reduction treatment. The morphological analysis reveals that numerous Ni-Co nanoparticles are densely packed inside the hollow interior of Ni 0.19 Co 0.26 P nanowires. This unique structure integrates the advantages of high surface area from the nanowires and enhanced electronic conductivity from the Ni-Co nanoparticles, effectively addressing the conventional issue of slow charge transfer rate in 3D metal-based compounds, which is typically caused by a long transfer distance. The electrocatalysts provide an excellent hydrogen evolution reaction performance in alkaline, neutral, and acidic media with an overpotential of 35, 36, and 23 mV to reach the current density of 10 mA/cm 2 , respectively. This study highlights the potential of Ni-Co nanoparticles@Ni 0.19 Co 0.26 P nanowires as a cost-effective and pH-universal HER electrocatalyst, offering promising prospects for sustainable hydrogen production.
摘要：
Transarterial radioembolization (TARE) using yttrium-90 ( 90 Y)-labeled glass and resin microspheres is an emerging therapeutic technique for hepatocellular carcinoma (HCC). However, the non-biodegradability and rapid settlement of current commercial microspheres might hinder their even distribution and repetitive administration thus causing unsatisfactory therapeutic effects. In this context, novel functional chitosan-based microspheres (CPIs) that can efficiently label Y as a favorable TARE material were developed for the first time by successive grafting poly (glycidyl methacrylate) (PGMA) and iminodiacetic acid (IDA) onto chitosan microspheres (CMs). The results confirmed that the CPIs had desirable spherical shapes with average diameters of around 20.9 μm, an ideal settlement rate within 5 min, and considerable biodegradability at 10th weeks. It reached Y adsorption equilibrium within 30 min and maintained the maximum adsorption capacity up to 14.95 mg g −1 at pH 6.0 following pseudo-second-order kinetic and Langmuir models. Additionally, Y-labeled CPIs were rather stable in vitro, for which Y would firmly interact with the sodium carboxylate group and tertiary amine nitrogen atoms on IDA, and its leakage when shaken in phosphate-buffered saline for 24 h was barely detected. Altogether, these properties of the as-developed CPIs hold great potential as promising radioembolization microspheres for TARE therapy against liver cancer.
Transarterial radioembolization (TARE) using yttrium-90 ( 90 Y)-labeled glass and resin microspheres is an emerging therapeutic technique for hepatocellular carcinoma (HCC). However, the non-biodegradability and rapid settlement of current commercial microspheres might hinder their even distribution and repetitive administration thus causing unsatisfactory therapeutic effects. In this context, novel functional chitosan-based microspheres (CPIs) that can efficiently label Y as a favorable TARE material were developed for the first time by successive grafting poly (glycidyl methacrylate) (PGMA) and iminodiacetic acid (IDA) onto chitosan microspheres (CMs). The results confirmed that the CPIs had desirable spherical shapes with average diameters of around 20.9 μm, an ideal settlement rate within 5 min, and considerable biodegradability at 10th weeks. It reached Y adsorption equilibrium within 30 min and maintained the maximum adsorption capacity up to 14.95 mg g −1 at pH 6.0 following pseudo-second-order kinetic and Langmuir models. Additionally, Y-labeled CPIs were rather stable in vitro, for which Y would firmly interact with the sodium carboxylate group and tertiary amine nitrogen atoms on IDA, and its leakage when shaken in phosphate-buffered saline for 24 h was barely detected. Altogether, these properties of the as-developed CPIs hold great potential as promising radioembolization microspheres for TARE therapy against liver cancer.
摘要：
To advance the application of Cu-metal–organic framework (Cu-MOF) in uranium wastewater treatment for the nuclear industry, it is essential to address the limitations of its powder form and rigid structure while enhancing its adsorption capacity. In this study, a Cu-MOF@CSTA composite adsorbent was synthesized by solidifying Cu-MOF with chitosan/tannic acid and modifying its functional groups to improve uranium adsorption performance. The U(VI) theoretical maximum adsorption capacity of Cu-MOF@CSTA at 288 K (pH = 5) was 2507.73 mg/g, and the adsorption process was characterized as a spontaneous exothermic reaction. The uranium removal rate in mine wastewater (pore water and seepage water) reached 100 %. Uranium removal efficiencies in wastewater containing 100 mg/L fluoride ions, ammonia ions, and urea were remarkably high at 99.34 %, 99.63 %, and 98.94 %, respectively, demonstrating the composite adsorbent’s robust anti-jamming capability. Mechanistic analysis revealed that the synergistic effects of hydroxyl, amino, and sulfur functional groups on the Cu-MOF@CSTA surface facilitated uranium adsorption. Competitive adsorption experiments confirmed that Cu-MOF@CSTA exhibits excellent selectivity for uranium. The chitosan, solidified by tannic acid as a flexible carrier, stabilized the Cu-MOF, highlighting its significant potential for uranium adsorption in wastewater treatment applications.
To advance the application of Cu-metal–organic framework (Cu-MOF) in uranium wastewater treatment for the nuclear industry, it is essential to address the limitations of its powder form and rigid structure while enhancing its adsorption capacity. In this study, a Cu-MOF@CSTA composite adsorbent was synthesized by solidifying Cu-MOF with chitosan/tannic acid and modifying its functional groups to improve uranium adsorption performance. The U(VI) theoretical maximum adsorption capacity of Cu-MOF@CSTA at 288 K (pH = 5) was 2507.73 mg/g, and the adsorption process was characterized as a spontaneous exothermic reaction. The uranium removal rate in mine wastewater (pore water and seepage water) reached 100 %. Uranium removal efficiencies in wastewater containing 100 mg/L fluoride ions, ammonia ions, and urea were remarkably high at 99.34 %, 99.63 %, and 98.94 %, respectively, demonstrating the composite adsorbent’s robust anti-jamming capability. Mechanistic analysis revealed that the synergistic effects of hydroxyl, amino, and sulfur functional groups on the Cu-MOF@CSTA surface facilitated uranium adsorption. Competitive adsorption experiments confirmed that Cu-MOF@CSTA exhibits excellent selectivity for uranium. The chitosan, solidified by tannic acid as a flexible carrier, stabilized the Cu-MOF, highlighting its significant potential for uranium adsorption in wastewater treatment applications.
作者机构：
[Meifang Wang] Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha 410208, China;[Hongying Bu] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[Weijia Luo; Xi Zeng] Hunan Engineering Research Center for Early Diagnosis and Treatment of Liver Cancer, Hunan Province Key Laboratory of Tumor Cellular & Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China;[Guodong Chen] Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital, Hengyang Medical School, University of South China, 28 West Changsheng Road, Hengyang, Hunan 421001, China;[Yingchun He] Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Hunan University of Chinese Medicine, Changsha 410208, China
通讯机构：
[Yingchun He] H;Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Hunan University of Chinese Medicine, Changsha 410208, China
摘要：
Rheumatoid arthritis (RA) is a common chronic autoimmune disease that primarily affects the joints, leading to synovial inflammation and hyperplasia, which subsequently causes joint pain, swelling, and damage. The microenvironment of RA is characterized by hypoxia, high reactive oxygen species (ROS), low pH, and levels of high inflammatory factors. Traditional treatments only partially alleviate symptoms and often cause various adverse reactions with long-term use. Therefore, there is an urgent need for safer and more effective treatments. In recent years, mesenchymal stem cells (MSCs) have shown significant potential in treating RA due to their diverse immunomodulatory mechanisms. MSCs paracrine a variety of soluble factors to improve the inflammatory microenvironment in RA patients by inhibiting T cell proliferation or inducing T cell differentiation to regulatory T cells (Tregs), inhibiting B cell proliferation and differentiation and immunoglobulin production, prompting macrophage polarization toward an anti-inflammatory phenotype, and inhibiting neutrophil recruitment and preventing the maturation of dendritic cells (DCs). This review summarizes the immunomodulatory effects of MSCs in RA and their application in animal models and clinical trials. Although the immunomodulatory mechanisms of MSCs are not yet fully elucidated, their significant potential in RA treatment has been widely recognized. Future research should further explore the immunomodulatory mechanisms of MSCs and optimize their functions in different pathological microenvironments to develop more effective and safer therapeutic strategies.
Rheumatoid arthritis (RA) is a common chronic autoimmune disease that primarily affects the joints, leading to synovial inflammation and hyperplasia, which subsequently causes joint pain, swelling, and damage. The microenvironment of RA is characterized by hypoxia, high reactive oxygen species (ROS), low pH, and levels of high inflammatory factors. Traditional treatments only partially alleviate symptoms and often cause various adverse reactions with long-term use. Therefore, there is an urgent need for safer and more effective treatments. In recent years, mesenchymal stem cells (MSCs) have shown significant potential in treating RA due to their diverse immunomodulatory mechanisms. MSCs paracrine a variety of soluble factors to improve the inflammatory microenvironment in RA patients by inhibiting T cell proliferation or inducing T cell differentiation to regulatory T cells (Tregs), inhibiting B cell proliferation and differentiation and immunoglobulin production, prompting macrophage polarization toward an anti-inflammatory phenotype, and inhibiting neutrophil recruitment and preventing the maturation of dendritic cells (DCs). This review summarizes the immunomodulatory effects of MSCs in RA and their application in animal models and clinical trials. Although the immunomodulatory mechanisms of MSCs are not yet fully elucidated, their significant potential in RA treatment has been widely recognized. Future research should further explore the immunomodulatory mechanisms of MSCs and optimize their functions in different pathological microenvironments to develop more effective and safer therapeutic strategies.
摘要：
Bacterial contamination poses significant threats to public health through food safety issues, creating a critical need for rapid and sensitive bacterial detection platforms. Herein, we developed a novel label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification, using Escherichia coli (E. coli) as a proof of concept. We engineered a hairpin-structured capture probe integrating an E. coli-specific aptamer with an HCR trigger sequence, achieving fluorescence amplification through three synergistic mechanisms: (1) HCR-driven DNA duplex assembly; (2) enhanced SYBR Green I (SG-I, a cost-effective non-labeled dye) intercalation into HCR-generated DNA duplexes; and (3) target-selective fluorescence modulation via MnO(2) nanosheets that quench background fluorescence through preferential adsorption of single-stranded DNA (ssDNA)-bound SG-I over duplex DNA-intercalated dye, exploiting the differential binding affinity of MnO(2) for ssDNA versus double-stranded DNA. This strategy enabled a fluorescence sensor with high sensitivity (detection limit: 17CFU/mL), excellent specificity, and broad dynamic range (5.0 × 10(1) ~ 5.0 × 10(7)CFU/mL), demonstrating robust performance in complex food matrices through successful E. coli detection in spiked milk and lettuce samples with recoveries of 98.81 to 104.26%, thereby underscoring the method's reliability and practical utility for on-site food safety monitoring in real-world scenarios.
期刊：
Journal of Hazardous Materials Advances,2025年19:100839 ISSN：2772-4166
通讯作者：
Jun Qiu
作者机构：
[Yin, Jieyun] Department of Epidemiology and Health Statistics, Medical College of Soochow University, Suzhou 215123, China;[Pan, Xiongfeng; Xiang, Shiting; Liu, Caixia; Qiu, Jun] Pediatrics Research Institute of Hunan Province, Hunan Children's Hospital, Changsha 410007, China;[Zhao, Kunyan; Yang, Fei] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, China;[Wu, Sha; Cao, Yunhui] Department of Pediatrics, Hengyang Medical School, University of South China, Hengyang 421001, China;[Wu, Mingyang] Department of Maternal and Child Health, Xiangya School of Public Health, Central South University, Changsha 410007, China
通讯机构：
[Jun Qiu] P;Pediatrics Research Institute of Hunan Province, Hunan Children's Hospital, Changsha 410007, China
关键词：
Weight;Child growth;Metals;Gut microbiota
摘要：
Backgrounds Research indicates that metal exposure and gut microbiota may influence weight gain in children. This study aimed to examine the relationship between metal mixture exposure, gut microbiota composition, and weight gain trajectories among children admitted to the neonatal intensive care unit.
Research indicates that metal exposure and gut microbiota may influence weight gain in children. This study aimed to examine the relationship between metal mixture exposure, gut microbiota composition, and weight gain trajectories among children admitted to the neonatal intensive care unit.
Methods A total of 207 children were recruited from the Children's Hospital of Hunan Province, Central China. Blood metal concentrations were measured using ICP-MS, and gut microbiota composition was determined through 16S rRNA gene sequencing. Group-based trajectory modeling, logistic regression and Bayesian Kernel Machine Regression (BKMR) were used to assess the effects of metal mixtures and gut microbiota on children's weight gain patterns.
A total of 207 children were recruited from the Children's Hospital of Hunan Province, Central China. Blood metal concentrations were measured using ICP-MS, and gut microbiota composition was determined through 16S rRNA gene sequencing. Group-based trajectory modeling, logistic regression and Bayesian Kernel Machine Regression (BKMR) were used to assess the effects of metal mixtures and gut microbiota on children's weight gain patterns.
Results Four distinct weight gain trajectories were identified over the five-year follow-up: growth retardation group, catch-up growth group, high-end weight group, and normal growth group. Among 26 metals, Cs ( OR = 3.208, 95 % CI : 1.107, 9.963), Cu ( OR = 3.270, 95 % CI : 1.132, 10.148), W ( OR = 3.393, 95 % CI : 1.208, 10.101), As ( OR = 0.272, 95 % CI : 0.069, 0.872) and Mn ( OR = 0.201, 95 % CI : 0.047, 0.674) were significantly correlated with the growth retardation group. RB41 ( OR = 9.630, p = 0.016) and Bacteroides ( OR =0.272, p = 0.011) in gut microbiota were correlated with the growth retardation group.
Four distinct weight gain trajectories were identified over the five-year follow-up: growth retardation group, catch-up growth group, high-end weight group, and normal growth group. Among 26 metals, Cs ( OR = 3.208, 95 % CI : 1.107, 9.963), Cu ( OR = 3.270, 95 % CI : 1.132, 10.148), W ( OR = 3.393, 95 % CI : 1.208, 10.101), As ( OR = 0.272, 95 % CI : 0.069, 0.872) and Mn ( OR = 0.201, 95 % CI : 0.047, 0.674) were significantly correlated with the growth retardation group. RB41 ( OR = 9.630, p = 0.016) and Bacteroides ( OR =0.272, p = 0.011) in gut microbiota were correlated with the growth retardation group.
Conclusion Mn, As, Cs, Cu, W, Zn and Sb in 26 metals, RB41 and Bacteroides in gut microbiota were associated with the trajectory patterns of children. Maintaining optimal blood metal levels and regulating gut microbiota composition during early infancy may have important implications for promoting healthy weight gain trajectories in early childhood.
Mn, As, Cs, Cu, W, Zn and Sb in 26 metals, RB41 and Bacteroides in gut microbiota were associated with the trajectory patterns of children. Maintaining optimal blood metal levels and regulating gut microbiota composition during early infancy may have important implications for promoting healthy weight gain trajectories in early childhood.
作者机构：
[Wu, Yimou; Zhao, Feijun; Liu, Zhaoping] Univ South China, Inst Microbiol & Infect Dis, Hunan Prov Clin Res Ctr Accurate Diag & Treatment, Affiliated Hosp 1,Hengyang Med Sch,Dept Clin Lab M, Hengyang, Hunan, Peoples R China.;[Wu, Yimou; Gao, Ke; Zhao, Feijun; Ding, Xuan; Yu, Han; Yao, Jiangchen; Zhang, Xiaohong] Univ South China, Hengyang Med Coll, MOE Key Lab of Rare Pediat Dis, Hengyang 421001, Peoples R China.;[Wu, Yimou; Gao, Ke; Zhao, Feijun; Ding, Xuan; Yu, Han; Yao, Jiangchen; Zhang, Xiaohong] Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Hengyang 421001, Peoples R China.;[Wu, Yimou; Gao, Ke; Zhao, Feijun; Ding, Xuan; Yu, Han; Yao, Jiangchen; Zhang, Xiaohong] Univ South China, Hengyang Med Coll, Key Lab Special Pathogen Prevent & Control Hunan P, Hengyang 421001, Peoples R China.;[Zhao, Feijun] Changsha Cent Hosp, Dept Clin Lab Med, Changsha 410004, Peoples R China.
通讯机构：
[Zhao, FJ ; Wu, YM] U;Univ South China, Hengyang Med Coll, MOE Key Lab of Rare Pediat Dis, Hengyang 421001, Peoples R China.;Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Hengyang 421001, Peoples R China.;Univ South China, Hengyang Med Coll, Key Lab Special Pathogen Prevent & Control Hunan P, Hengyang 421001, Peoples R China.;Univ South China, Inst Microbiol & Infect Dis, Hunan Prov Clin Res Ctr Accurate Diag & Treatment, Affiliated Hosp 1,Hengyang Med Sch,Dept Clin Lab M, Hengyang, Hunan, Peoples R China.
摘要：
Neurosyphilis (NS) is a chronic central nervous system infection caused by Treponema pallidum. Owing to its diverse clinical manifestations and the limited sensitivity of current diagnostic methods, NS is difficult to diagnose. Understanding the molecular mechanisms of NS and identifying reliable biomarkers are essential for improving diagnostic and therapeutic strategies. This study employed Mendelian randomization (MR) analysis to explore the causal relationships among protein ratio quantitative trait loci (rQTLs), cerebrospinal fluid (CSF) metabolites, and syphilis risk at various stages. The results revealed that several rQTLs, including CD46/TNFRSF14 and TBC1D23/TBC1D5, were closely associated with syphilis risk, whereas others, such as BANK1/HEXIM1 and GOPC/HEXIM1, exhibited protective effects. Mediation analysis further identified key CSF metabolites, such as N-acetyltaurine and bilirubin, as important mediators linking rQTLs and syphilis progression. Through integrated analysis of cis-proteins from rQTLs and transcriptomic data from CD4 + T-cells of NS patients, METAP2 was identified as a key biomarker in NS, with the potential mechanisms elucidated. Importantly, T. pallidum may inhibit CD4 + T-cell proliferation by modulating METAP2, thereby accelerating disease progression. These findings offer new insights into the pathogenesis of NS and highlight METAP2 as a potential biomarker, laying a foundation for improving diagnostic and therapeutic strategies.
