期刊：
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM,2024年109(6):1540-1549 ISSN：0021-972X
通讯作者：
Zheng, MH;Valenti, L
作者机构：
[Liu, Wen-Yue] Wenzhou Med Univ, Affiliated Hosp 1, Dept Endocrinol, Wenzhou 325000, Peoples R China.;[Liu, Wen-Yue] Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou Key Lab Diabet Res, Wenzhou 325000, Peoples R China.;[Zhang, Ni; Lian, Li-You; Ye, Chen-Hui; Zheng, Ming-Hua; Chen, Wen-Ying] Wenzhou Med Univ, Affiliated Hosp 1, MAFLD Res Ctr, Dept Hepatol, Wenzhou 325000, Peoples R China.;[Zhang, Ni; Lian, Li-You; Ye, Chen-Hui; Zheng, Ming-Hua; Chen, Wen-Ying] Wenzhou Med Univ, Inst Hepatol, Wenzhou 325000, Peoples R China.;[Zhang, Ni; Lian, Li-You; Ye, Chen-Hui; Zheng, Ming-Hua; Chen, Wen-Ying] Key Lab Diag & Treatment Dev Chron Liver Dis, Wenzhou, Zhejiang, Peoples R China.
通讯机构：
[Zheng, MH ] W;[Valenti, L ] U;Wenzhou Med Univ, Affiliated Hosp 1, MAFLD Res Ctr, Dept Hepatol, 2 Fuxue Lane, Wenzhou 325000, Peoples R China.;Univ Milan, Dept Pathophysiol & Transplantat, Via Festa Perdono 7, I-20121 Milan, Italy.
关键词：
iron overload;metabolic dysfunction–associated fatty liver disease;metabolic hyperferritinemia;metabolic syndrome
摘要：
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Context</jats:title>
<jats:p>There is limited data on the clinical significance of metabolic hyperferritinemia (MHF) based on the most recent consensus.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Objective</jats:title>
<jats:p>We aimed to validate the clinical outcomes of MHF in the general population and patients with biopsy-proven metabolic dysfunction–associated fatty liver disease (MAFLD).</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods</jats:title>
<jats:p>The NHANES database and PERSONS cohort were included. MHF was defined as elevated serum ferritin with metabolic dysfunction (MD) and stratified into different grades according to ferritin (grade 1: 200 [females]/300 [males]—550 ng/mL; grade 2: 550-1000 ng/mL; grade 3: &gt;1000 ng/mL). The clinical outcomes, including all-cause death, comorbidities, and liver histology, were compared between non-MHF and MHF in adjusted models.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>In NHANES, compared with non-MHF with MD, MHF was related to higher risks of advanced fibrosis (P = .036), elevated albumin–creatinine ratio (UACR, P = .001), and sarcopenia (P = .013). Although the association between all grades of MHF and mortality was insignificant (P = .122), grades 2/3 was associated with increased mortality (P = .029). When comparing with non-MHF without MD, the harmful effects of MHF were more significant in mortality (P &lt; .001), elevated UACR (P &lt; .001), cardiovascular disease (P = .028), and sarcopenia (P &lt; .001). In the PERSONS cohort, MHF was associated with more advanced grades of steatosis (P &lt; .001), lobular inflammation (P &lt; .001), advanced fibrosis (P = .017), and more severe hepatocellular iron deposition (P &lt; .001).</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusion</jats:title>
<jats:p>Both in the general population and in at-risk individuals with MAFLD, MHF was related with poorer clinical outcomes.</jats:p>
</jats:sec>
作者机构：
[Zheng, Xingyu; Chen, Jun; Wu, Qian; Qu, Xiaowang; Liu, Wenpei; Pan, Dong; Teng, Shishan; Liu, Bo; Peng, Liting; Lu, Rui; He, Rongzhang; Hu, Yabin; Yang, Jing] Univ South China, Coll Basic Med Sci, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Zheng, Xingyu; Chen, Jun; Wu, Qian; Qu, Xiaowang; Liu, Wenpei; Pan, Dong; Teng, Shishan; Liu, Bo; Peng, Liting; Lu, Rui; He, Rongzhang; Hu, Yabin; Yang, Jing] MOE, Key Lab Rare Pediat Dis, Hengyang 421001, Peoples R China.;[Chen, Jun; Hu, Yabin] Univ South China, Peoples Hosp Chenzhou 1, Translat Med Inst, Hengyang Med Sch, Chenzhou 423000, Peoples R China.;[Chen, Hongying; Liu, Zhanpeng; Zhang, Xiaoyue; Liu, Lulu] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China.;[Wu, Qian; Li, Yi-Ping] Sun Yat sen Univ, Inst Human Virol, Dept Pathogen Biol & Biosecur, Guangzhou 510080, Peoples R China.
通讯机构：
[Qu, XW ; Liu, WP] U;[Chen, HY ] N;[Li, YP ] S;Univ South China, Coll Basic Med Sci, Hengyang Med Sch, Hengyang 421001, Peoples R China.;MOE, Key Lab Rare Pediat Dis, Hengyang 421001, Peoples R China.
关键词：
MONOCLONAL;acute
摘要：
Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),theseventh coronavirus known to jump f...展开更多 Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),theseventh coronavirus known to jump from intermediate hosts to humans,has resulted in a worldwide pandemic and caused immense economicdamage(Tan et al.,2020;Zhu et al.,2020).It is believed that coronavirus spillover may occur again in the future.Human exposure to a bat coro-navirus has been serologically confimed in a rural area of China,indi-cating that the coronavirus spillover occurred,although the origin andfrequency of spillovers are unclear(Wang et al.,2018).收起
通讯机构：
[Yang, SY ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Hunan, Peoples R China.
摘要：
Herein, we present a paper-based POCT sensor based on lactate dehydrogenase-mediated alginate gelation combined with visual distance reading and smartphone-assisted colorimetric dual-signal analysis to determine the concentration of (L)-lactate in yogurt samples. In this research, (L)-lactate was transformed into pyruvate by lactate dehydrogenase. Pyruvate then triggered the gelation of a sol mixture, increasing the viscosity (eta(s)) of the mixture, which was shown as a decrease in the diffusion diameter on the paper-based sensor. In addition, protons from pyruvate accelerated the degradation of Rhodamine B, causing color fading of the mixture, which was analyzed using RGB analysis application software. Under optimal experimental conditions, the linear ranges of visual distance reading and smartphone-assisted colorimetric analysis were 0.1-15 mu M and 0.3-15 mu M and the detection limits were 0.03 mu M and 0.07 mu M, respectively. As a proof-of-concept application, we exploited the paper-based sensor to determine the concentration of (L)-lactate in yogurt samples. The results from the dual-signal paper-based sensor were consistent with the ones from HPLC analysis. In short, this study developed a simple, convenient, cost-effective, and feasible method for the quantitative detection of (L)-lactate in real samples.
通讯机构：
[Zhao, YX ] C;[Hao, YE ] U;Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Resources Trop Crops, Haikou 571101, Peoples R China.;Hainan Inst Trop Agr Resources, Key Lab Biol & Resources Trop Crops Hainan Prov, Haikou 571101, Peoples R China.;Univ South China, Sch Publ Hlth, Heng yang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.
关键词：
16S rRNA gene;Phytohabitans maris sp. nov.;marine sediment;polyphasic taxonomy
摘要：
A novel actinobacterium, strain ZYX- F - 186( T) , was isolated from marine sediment sampled on Yongxing Island, Hainan Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain ZYX- F - 186(T) belongs to the genus Phytohabitans , with high similarity to Phytohabitans kaempferiae KK1-3(T) (98.3 %), Phytohabitans rumicis K11 -0047(T) (98.1 %), Phytohabitans flavus K09 -0627(T) (98.1 %), Phytohabitans houttuyneae K11 -0057(T) (97.9 %), Phytohabitans suffuscus K07 -0523(T) (97.7 %), and Phytohabitans aurantiacus RD004123(T) (97.7 %). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a single subclade in the genus Phytohabitans . The novel isolate contained meso -diaminopimelic acid, o- glutamic acid, glycine, o-alanine, and l - lysine in the cell wall. The whole - cell sugars were xylose, arabinose, ribose, and rhamnose. The predominant menaquinones were MK- 9(H-8 ), MK -9(H-6 ), and MK- 9(H-4 ). The characteristic phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The major fatty acids (>5 %) were iso- C 16:0 , anteiso- C 17:0 , and iso- C 18:0 . Genome sequencing showed a DNA G+C content of 71.9 mol%. Low average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values demonstrated that strain ZYX- F - 186(T) could be readily distinguished from its closely related species. Based on its phylogenetic, chemotaxonomic, and physiological characteristics, strain ZYX-F-186(T) represents a novel species of the genus Phytohabitans , for which the name Phytohabitans maris sp. nov. is proposed. The type strain is ZYX- F - 186(T) (=CGMCC 4.8025(T) =CCTCC AA 2023025(T) =JCM 36507(T) ).
摘要：
<jats:title>Abstract</jats:title><jats:p>Tumor cell metastasis is the key cause of death in patients with nasopharyngeal carcinoma (NPC). MiR‐2110 was cloned and identified in Epstein–Barr virus (EBV)‐positive NPC, but its role is unclear in NPC. In this study, we investigated the effect of miR‐2110 on NPC metastasis and its related molecular basis. In addition, we also explored whether miR‐2110 can be regulated by cinobufotalin (CB) and participate in the inhibition of CB on NPC metastasis. Bioinformatics, RT‐PCR, and in situ hybridization were used to observe the expression of miR‐2110 in NPC tissues and cells. Scratch, Boyden, and tail vein metastasis model of nude mouse were used to detect the effect of miR‐2110 on NPC metastasis. Western blot, Co‐IP, luciferase activity, colocalization of micro confocal and ubiquitination assays were used to identify the molecular mechanism of miR‐2110 affecting NPC metastasis. Finally, miR‐2110 induced by CB participates in CB‐stimulated inhibition of NPC metastasis was explored. The data showed that increased miR‐2110 significantly suppresses NPC cell migration, invasion, and metastasis. Suppressing miR‐2110 markedly restored NPC cell migration and invasion. Mechanistically, miR‐2110 directly targeted FGFR1 and reduced its protein expression. Decreased FGFR1 attenuated its recruitment of NEDD4, which downregulated NEDD4‐induced phosphatase and tensin homolog (PTEN) ubiquitination and degradation and further increased PTEN protein stability, thereby inactivating PI3K/AKT‐stimulated epithelial–mesenchymal transition signaling and ultimately suppressing NPC metastasis. Interestingly, CB, a potential new inhibitory drug for NPC metastasis, significantly induced miR‐2110 expression by suppressing PI3K/AKT/c‐Jun‐mediated transcription inhibition. Suppression of miR‐2110 significantly restored cell migration and invasion in CB‐treated NPC cells. Finally, a clinical sample assay indicated that reduced miR‐2110 was negatively correlated with NPC lymph node metastasis and positively related to NPC patient survival prognosis. In summary, miR‐2110 is a metastatic suppressor involving in CB‐induced suppression of NPC metastasis.</jats:p>
期刊：
Biosensors and Bioelectronics,2024年267:116756 ISSN：0956-5663
通讯作者：
Xiangheng Niu
作者机构：
[Niu, Xiangheng; Zhu, Hengjia] School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China;[Niu, Xiangheng; Zhu, Hengjia] School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China;[Liu, Bangxiang; Hu, Panwang] School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China;[Pan, Jianming] School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address: pjm@ujs.edu.cn;[Xu, Lizhang] School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address: justxlz@ujs.edu.cn
通讯机构：
[Xiangheng Niu] S;School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, PR China<&wdkj&>School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, PR China<&wdkj&>School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, PR China<&wdkj&>School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164, USA
摘要：
In view of the current serious situation of organophosphorus pesticides (OPs) residue contamination, developing rapid and accurate OPs sensors is a matter of urgency. Redox-nanozyme based colorimetric sensors have been widely researched and utilized in OPs residue determination, but overcoming the interference of external redox substances and the effect of single-signal modes on detection performance is still a challenge. Here we fabricated a Zr-based metal-organic framework (MOF) featuring specific phosphatase-like activity and strong aggregation-induced emission (AIE) fluorescence for redox interference-free bimodal pesticide sensing. In the MOF, the activity-tunable Zr(4+) node offered high hydrolytic activity and affinity toward P-O containing substrates, and the rigid framework structure effectively enhanced the fluorescence emission of the ligand 1,1,2,2-tetra(4-carboxylphenyl)ethylene. The developed AIEzyme could efficiently catalyze the hydrolysis of paraoxon to yellow p-nitrophenol, which further reduced the intrinsic AIE fluorescence of AIEzyme through internal filtration effect. Thereby, a natural enzyme-free dual-mode colorimetric/fluorescence approach was established for paraoxon detection with no interference from redox substances, and a smartphone-assisted portable platform was further developed to enable the facile, rapid, and high-performance sensing of the pesticide in complex practical matrices.
期刊：
ENVIRONMENTAL HEALTH PERSPECTIVES,2024年132(5):57005 ISSN：0091-6765
作者机构：
[Zhou, Lu; Gao, Ya; Lei, Jian; Zhu, Yixiang; Liu, Cong; He, Cheng; Chen, Renjie; Kan, Haidong] Shanghai Institute of Infectious Disease and Biosecurity, School of Public Health, Fudan University, Shanghai, China;[He, Cheng] Institute of Epidemiology, Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH), Neuherberg, Germany;[Xuan, Jianwei] Health Economic Research Institute, School of Pharmacy, Sun Yat-Sen University, Guangzhou, China;[Kan, Haidong] National Center for Children's Health, Children's Hospital of Fudan University, Shanghai, China;[Chen, Renjie] School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China
摘要：
BACKGROUND: Although ambient temperature has been linked with injury incidence, there have been few nationwide studies to quantify the temperature-related risk and burden of cause-specific injury hospitalizations. Additionally, the impact of human-induced climate change to injury burden remains unknown. OBJECTIVES: Our objectives are to examine the associations between ambient temperature and injury hospitalizations from various causes and to quantify the contribution of human-induced warming to the heat-related burden. METHODS: We collected injury hospitalization data from a nationwide hospital-based registry in China during 2000-2019. Using a time-stratified case-crossover design, we investigated the associations between daily mean temperature (°C) and cause-specific injury hospitalizations. We also quantified the burden of heat-related injuries under the scenarios with and without anthropogenic forcing, using the Detection and Attribution Model Intercomparison Project to assess the contribution of human-induced warming. RESULTS: Our study included a total of 988,087 patients with hospitalization records for injuries. Overall, compared to the temperature at minimum risk of hospitalization ( -12.1°C), the relative risk of hospitalization at extreme hot temperature (30.8°C, 97.5th percentile) was 1.18 [95% confidence interval (CI): 1.14, 1.22], with an approximately linear association between temperature and hospitalization. Vulnerability to heat-related injuries was more pronounced among males, young (<18 years of age) or middle-aged (45-64 years of age) individuals, and those living in the North. The heat-related attributable fraction increased from 23.2% in the 2000s to 23.6% in the 2010s, with a corresponding increase in the contribution of human-induced change over time. In the 2010s, the heat-related attributable fractions for specific causes of injury ranged from 12.4% to 54.4%, with human-induced change accounting for 6.7% to 10.6% of the burden. DISCUSSION: This nationwide study presents new evidence of significant associations between temperature and cause-specific injury hospitalizations in China and highlights the increasing contribution of human-induced warming to the injury burden. https://doi.org/10.1289/EHP14057.
摘要：
Crystal violet (CV), widely used as a fungicide in aquaculture, is susceptible to enrichment in fish and thus poses a threat to human health. Therefore, there is an urgent need for a simple, sensitive and cost-effective method to detect CV. To this need, duck egg white-stabilized gold-silver nanoclusters (DEW-AuAgNCs) with long wavelength fluorescence emission were synthesized via microwave method in 1 min. The raw materials used were easily available and environmentally friendly. And based on the Fluorescence resonance energy transfer (FRET) between DEW-AuAgNCs and CV, a fluorescence quenching method was established for CV detection. The detection limit of this method was 1.7 × 10−8 mol/L, which was lower than most of the reported methods. In addition, prominent performance of DEW-AuAgNCs with long wavelength emission can be applied for the detection of CVs in real organism.
Crystal violet (CV), widely used as a fungicide in aquaculture, is susceptible to enrichment in fish and thus poses a threat to human health. Therefore, there is an urgent need for a simple, sensitive and cost-effective method to detect CV. To this need, duck egg white-stabilized gold-silver nanoclusters (DEW-AuAgNCs) with long wavelength fluorescence emission were synthesized via microwave method in 1 min. The raw materials used were easily available and environmentally friendly. And based on the Fluorescence resonance energy transfer (FRET) between DEW-AuAgNCs and CV, a fluorescence quenching method was established for CV detection. The detection limit of this method was 1.7 × 10−8 mol/L, which was lower than most of the reported methods. In addition, prominent performance of DEW-AuAgNCs with long wavelength emission can be applied for the detection of CVs in real organism.
摘要：
Pathogenic bacteria significantly contribute to elevated morbidity and mortality rates, highlighting the urgent need for early and precise detection. Currently, there is a paucity of effective broad-spectrum methods for detecting pathogenic bacteria. We have developed an innovative proton-responsive series piezoelectric quartz crystal (PR-SPQC) platform for the broad-spectrum identification of pathogenic bacteria. This was achieved by retrieving and aligning sequences from the NCBI GenBank database to identify and validate 16S rRNA oligonucleotide sequences that are signatures of pathogenic bacteria but absent in humans or fungi. The hyperbranched rolling circle amplification, activated exclusively by the screened target, exponentially generates protons that are detected by SPQC through a 2D polyaniline (PANI) film. The PR-SPQC platform demonstrates broad-spectrum capabilities in detecting pathogenic bacteria, with a detection limit of 2 CFU/mL within 90 min. Clinical testing of blood samples yielded satisfactory results. With its advantages in miniaturization, cost efficiency, and suitability for point-of-care testing, PR-SPQC has the potential to be extensively used for the rapid identification of diverse pathogenic bacteria within clinical practice and public health sectors.
作者机构：
[Li, Jingxian; Wang, Futing; Yang, Yan; Xiang, Shi; Cai, Ren; Tan, Weihong] Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Coll Biol,State Key Lab Chemo Biosensing & Chemome, Changsha 410082, Peoples R China.;[Tan, Weihong] Univ Chinese Acad Sci, Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp,Hangzhou Inst Med HIM, Hangzhou 310022, Zhejiang, Peoples R China.;[Tan, Weihong] Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med, Shanghai 200240, Peoples R China.;[Tan, Weihong] Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China.;[Yang, Hongfen; Yang, HF] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构：
[Yang, HF ] U;[Cai, R ] H;Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Coll Biol,State Key Lab Chemo Biosensing & Chemome, Changsha 410082, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
摘要：
PdPt nanosheets decorated on SnS(2) nanosheets (i.e., PdPt@SnS(2) NSs) were fabricated for a novel electrochemiluminescence (ECL) biosensor for ultrasensitive detection of miRNA-21 based on catalytic hairpin assembly (CHA) cycles. The PdPt@SnS(2) NSs serve as both the main luminophore and a highly effective coreaction accelerator in the ECL biosensor. In the CHA cycles, more miRNA-21 is captured, and the performance of the ECL biosensor is improved. When miRNA-21 is present, the hairpin chain DNA1 (i.e., H1) is opened, and the ferrocene (Fc)-modified hairpin chain DNA2 (i.e., Fc-H2) hybridizes with as-opened H1 by replacing miRNA-21 to stimulate CHA cycles of miRNA-21. During the CHA cycles, Fc-H2 quenches the ECL signal to monitor miRNA-21. As a result, the ECL biosensor shows ultrasensitive and highly selective detection of miRNA-21 from 1 aM to 1 nM with a detection limit (LOD) of 0.02 aM. In addition, the ECL biosensor exhibits excellent practicality for miRNA-21 detection in human serum samples.
作者机构：
[Wang, Jingyu; Bai, Qinqin; Liang, H; Zheng, Yi; Zhao, Fengxia; Liang, Hao; Yan, Hangli; Wu, Linghao; Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.;[Hu, Hongmei] Hengyang Ctr Dis Control & Prevent, Hengyang 421001, Peoples R China.
通讯机构：
[Liang, H ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.
摘要：
The detection of foodborne pathogens is crucial for food hygiene regulation and disease diagnosis. Colorimetry has become one of the main analytical methods in studying foodborne pathogens due to its advantages of visualization, low cost, simple operation, and no complex instrument. However, the low sensitivity limits its applications in early identification and on-site detection for trace analytes. In order to overcome such a limitation, herein we propose a joint strategy featuring dual signal amplification based on the hybridization chain reaction (HCR) and DNA-enhanced peroxidase-like activity of gold nanoparticles (AuNPs) for the sensitive visual detection of Escherichia coli. Target bacteria bound specifically to the aptamer domain in the capture hairpin probe, exposing the trigger domain for HCR and forming the extended double-stranded DNA (dsDNA) structures. The peroxidase-like catalytic capacity of AuNPs can be enhanced significantly by dsDNAs with the sticky ends of dsDNAs being adsorbed on AuNPs and the rigidity of dsDNAs causing the spatial regulation of AuNP concentration. The intensity of the enhancement was linearly related to the number of target bacteria. With the above strategy, the detection limit of our colorimetric method for Escherichia coli was down to 28 CFU mL(-1) within a short analytical time (50 min). This study provides a new perspective for the sensitive and visual detection of early bacterial contamination in foods.
摘要：
To ensure workforce stability in China’s healthcare system and maintain high-quality care, it is essential to comprehensively understand the interplay of factors contributing to turnover intention of healthcare workers. This study aims to examine the associations between potential factors and turnover intention in healthcare workers and explore the mediating effect of job satisfaction and work engagement in the association between them. In this cross-sectional study, a random sample of 1060 healthcare workers working in 98 public medical institutions were recruited to rate their turnover intention in 2018 in Shenzhen, China. Information on socio-demographic characteristics, job-related factors, turnover intention, job satisfaction, work engagement, work stress and doctor-patient relationship of participants were collected. Pearson’s chi-squared tests and binary logistic regression analyses were performed to explore the association between these factor and turnover intention. Mediation analysis was used to explore the roles of potential mediators and moderators. The results showed that age (OR: 0.35, 95%CI: 0.16 to 0.72), tenure (OR: 0.98, 95%CI: 0.96 to 0.99), administrative positions (OR: 0.33, 95%CI: 0.16 to 0.63), and night shift frequency (OR: 1.84, 95%CI: 1.26 to 2.67) were significantly associated with turnover intention. We identified the mediating effect of job satisfaction and work engagement in the relationship between administrative positions and turnover intention, while the suppressing effect in the relationship between professional titles and turnover intention. Additionally, we found that monthly income plays a moderating role in the relationship between work engagement and turnover intention, and in the association between professional titles and turnover intention. Greater job satisfaction and engagement, along with reasonable remuneration, were found to be associated with lower turnover intention among healthcare workers. Employers should proactively monitor the dynamic interactions among these factors and then develop more tailored interventions in order to alleviate the ongoing loss of healthcare workers.
摘要：
How to fabricate a multifunctional coating with controllable ultralow refractive index (RI), high antifogging and anticorrosion performances by a facile method is still one of the great challenges. In this work, a simple and time-saving approach involving a microwave-assisted sol-sel process was developed to fabricate a novel core-shell structured silica nanoparticle sol by co-condensation of perfluorodecyltriethoxysilane (F-DTOS) and tetraethoxysilane (TEOS) and self-condensation of F-DTOS. Results suggest that both the co-condensations and self-condensations not only give a layer of low surface energy -C10H4F17 groups on the silica particles but also enlarge the hybrid silica particle size, providing more and larger voids to form the high porosity. After spinning the silica nanoparticles on the substrate, the obtained optical coating exhibits the controllable ultralow refractive index (RI) and outstanding transparency. The minimum RI and maximum transmittance of our developed coating is up to similar to 1.04 and similar to 100 %, respectively. Moreover, because of the high porosity and the presence of low surface energy -C10H4F17 groups in the coating, the coating has the high hydrophobicity (water contact angle similar to 108.8 degrees), which not only makes it excellent antifogging performance but also affords it high-efficient anticorrosion property. After immersion in a 3.5 wt% NaCl solution for 7 days, the R-ct value of the coating could be higher than 7.3 x 10(4) Omega cm(2). It is believed that our developed method used to fabricate such multifunctional coating does not need any costly equipments, complex steps and time-consuming, making it well suited for industrial application.
通讯机构：
[Yang, HF ] U;[Cai, R ] H;Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Coll Biol,State Key Lab Chemo Biosensing & Chemome, Changsha 410082, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
摘要：
An ultrasensitive self-powered biosensor is constructed for miRNA-21 detection based on Au nanoparticles @ Pd nanorings (Au NPs@Pd NRs) and catalytic hairpin assembly (CHA). The Au NPs@Pd NRs possess excellent electrical conductivity to improve the electron transfer rate and show good elimination of byproduct H(2)O(2) to assist glucose oxidase (GOD) to catalyze glucose; CHA is used as an amplification strategy to effectively enhance the sensitivity of the biosensor. To further amplify the output signal, a capacitor is integrated into the self-powered biosensor. With multiple signal amplification strategies, the self-powered biosensor possesses a linear range of 0.1-10(-4) fM and a lower limit of detection (LOD) of 0.032 fM (S/N = 3). In addition, the as-prepared self-powered biosensor displays potential applicability in the assay toward miRNA-21 in human serum samples.
期刊：
Journal of Biological Chemistry,2024年300(8):107554 ISSN：0021-9258
通讯作者：
Guan, Hua;Zhou, PK;Lu, J
作者机构：
[Zhou, Ping-Kun; Guo, Hejiang; Han, Yang; Chen, Bijia; Yao, Shibo; Guan, Hua; Zhao, Hongling; Chen, Shi; Liu, Yuhao; Gao, Shanshan; Jia, Jin] Beijing Inst Radiat Med, Dept Radiat Biol, Beijing Key Lab Radiat Biol, Beijing, Peoples R China.;[Zhou, Ping-Kun; Chen, Shi; Jia, Jin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Lu, Jun] Capital Med Univ, Beijing YouAn Hosp, Dept Med Oncol, Lab Clin Med, Beijing, Peoples R China.
通讯机构：
[Guan, H; Zhou, PK ] B;[Lu, J ] C;Beijing Inst Radiat Med, Dept Radiat Biol, Beijing Key Lab Radiat Biol, Beijing, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;Capital Med Univ, Beijing YouAn Hosp, Dept Med Oncol, Lab Clin Med, Beijing, Peoples R China.
关键词：
Crotonylation;HR repair;PARP1;SIRT3;cGAS
摘要：
Cyclic GMP-AMP synthase (cGAS), a cytosolic DNA sensor, also exhibits nuclear genomic localization and is involved in DNA damage signaling. In this study, we investigated the impact of cGAS crotonylation on the regulation of the DNA damage response, particularly homologous recombination repair, following exposure to ionizing radiation (IR). Lysine 254 of cGAS is constitutively crotonylated by the CREB-binding protein; however, IR-induced DNA damage triggers sirtuin 3 (SIRT3)-mediated decrotonylation. Lysine 254 decrotonylation decreased the DNA-binding affinity of cGAS and inhibited its interaction with PARP1, promoting homologous recombination repair. Moreover, SIRT3 suppression led to homologous recombination repair inhibition and markedly sensitized cancer cells to IR and DNA-damaging chemicals, highlighting SIRT3 as a potential target for cancer therapy. Overall, this study revealed the crucial role of cGAS crotonylation in the DNA damage response. Furthermore, we propose that modulating cGAS and SIRT3 activities could be potential strategies for cancer therapy.
Cyclic GMP-AMP synthase (cGAS), a cytosolic DNA sensor, also exhibits nuclear genomic localization and is involved in DNA damage signaling. In this study, we investigated the impact of cGAS crotonylation on the regulation of the DNA damage response, particularly homologous recombination repair, following exposure to ionizing radiation (IR). Lysine 254 of cGAS is constitutively crotonylated by the CREB-binding protein; however, IR-induced DNA damage triggers sirtuin 3 (SIRT3)-mediated decrotonylation. Lysine 254 decrotonylation decreased the DNA-binding affinity of cGAS and inhibited its interaction with PARP1, promoting homologous recombination repair. Moreover, SIRT3 suppression led to homologous recombination repair inhibition and markedly sensitized cancer cells to IR and DNA-damaging chemicals, highlighting SIRT3 as a potential target for cancer therapy. Overall, this study revealed the crucial role of cGAS crotonylation in the DNA damage response. Furthermore, we propose that modulating cGAS and SIRT3 activities could be potential strategies for cancer therapy.
