摘要：
Pesticide sensing crucially safeguards food safety and public health against environmental and health hazards. While oxidoreductase-type nanozymes (peroxidase and oxidase) have been widely used in optical pesticide detection, their susceptibility to redox interference as well as poor target specificity limits practical applications. To overcome the deficiencies, here we developed Ca 2+ -chelated 2-aminoterephthalic acid on nanosized ceria (Ca-ATPA@CeO 2 ) as an organophosphorus hydrolase mimic. This design integrates stable fluorescence and dual-site catalytic activity to specifically detect methyl-paraoxon (MP) in complex food matrices. The synergy between Ca 2+ (hard Lewis acid) and CeO 2 creates dual active sites to catalyze MP hydrolysis into yellow p -nitrophenol (pNP), and the latter quenches nanozyme fluorescence via inner filter effect. The system enables cross-validated quantification of MP in complex samples, eliminating redox interference through target-specific catalysis. The bimodal “on” colorimetric (pNP color signal) and “off” fluorescence (nanozyme fluorescence intensity) detection achieved linear ranges within 1–200 μM, providing detection limits of 1.43 μM and 0.087 μM, respectively. Our work proposes a reliable strategy for selective MP detection that can avoid redox interference, also providing a simple yet efficient design of high-activity fluorescent hydrolase mimics with broadened applications in food safety analysis and beyond.
Pesticide sensing crucially safeguards food safety and public health against environmental and health hazards. While oxidoreductase-type nanozymes (peroxidase and oxidase) have been widely used in optical pesticide detection, their susceptibility to redox interference as well as poor target specificity limits practical applications. To overcome the deficiencies, here we developed Ca 2+ -chelated 2-aminoterephthalic acid on nanosized ceria (Ca-ATPA@CeO 2 ) as an organophosphorus hydrolase mimic. This design integrates stable fluorescence and dual-site catalytic activity to specifically detect methyl-paraoxon (MP) in complex food matrices. The synergy between Ca 2+ (hard Lewis acid) and CeO 2 creates dual active sites to catalyze MP hydrolysis into yellow p -nitrophenol (pNP), and the latter quenches nanozyme fluorescence via inner filter effect. The system enables cross-validated quantification of MP in complex samples, eliminating redox interference through target-specific catalysis. The bimodal “on” colorimetric (pNP color signal) and “off” fluorescence (nanozyme fluorescence intensity) detection achieved linear ranges within 1–200 μM, providing detection limits of 1.43 μM and 0.087 μM, respectively. Our work proposes a reliable strategy for selective MP detection that can avoid redox interference, also providing a simple yet efficient design of high-activity fluorescent hydrolase mimics with broadened applications in food safety analysis and beyond.
摘要：
Rheumatoid arthritis (RA) is a common chronic autoimmune disease that primarily affects the joints, leading to synovial inflammation and hyperplasia, which subsequently causes joint pain, swelling, and damage. The microenvironment of RA is characterized by hypoxia, high reactive oxygen species (ROS), low pH, and levels of high inflammatory factors. Traditional treatments only partially alleviate symptoms and often cause various adverse reactions with long-term use. Therefore, there is an urgent need for safer and more effective treatments. In recent years, mesenchymal stem cells (MSCs) have shown significant potential in treating RA due to their diverse immunomodulatory mechanisms. MSCs paracrine a variety of soluble factors to improve the inflammatory microenvironment in RA patients by inhibiting T cell proliferation or inducing T cell differentiation to regulatory T cells (Tregs), inhibiting B cell proliferation and differentiation and immunoglobulin production, prompting macrophage polarization toward an anti-inflammatory phenotype, and inhibiting neutrophil recruitment and preventing the maturation of dendritic cells (DCs). This review summarizes the immunomodulatory effects of MSCs in RA and their application in animal models and clinical trials. Although the immunomodulatory mechanisms of MSCs are not yet fully elucidated, their significant potential in RA treatment has been widely recognized. Future research should further explore the immunomodulatory mechanisms of MSCs and optimize their functions in different pathological microenvironments to develop more effective and safer therapeutic strategies.
Rheumatoid arthritis (RA) is a common chronic autoimmune disease that primarily affects the joints, leading to synovial inflammation and hyperplasia, which subsequently causes joint pain, swelling, and damage. The microenvironment of RA is characterized by hypoxia, high reactive oxygen species (ROS), low pH, and levels of high inflammatory factors. Traditional treatments only partially alleviate symptoms and often cause various adverse reactions with long-term use. Therefore, there is an urgent need for safer and more effective treatments. In recent years, mesenchymal stem cells (MSCs) have shown significant potential in treating RA due to their diverse immunomodulatory mechanisms. MSCs paracrine a variety of soluble factors to improve the inflammatory microenvironment in RA patients by inhibiting T cell proliferation or inducing T cell differentiation to regulatory T cells (Tregs), inhibiting B cell proliferation and differentiation and immunoglobulin production, prompting macrophage polarization toward an anti-inflammatory phenotype, and inhibiting neutrophil recruitment and preventing the maturation of dendritic cells (DCs). This review summarizes the immunomodulatory effects of MSCs in RA and their application in animal models and clinical trials. Although the immunomodulatory mechanisms of MSCs are not yet fully elucidated, their significant potential in RA treatment has been widely recognized. Future research should further explore the immunomodulatory mechanisms of MSCs and optimize their functions in different pathological microenvironments to develop more effective and safer therapeutic strategies.
期刊：
Science of The Total Environment,2025年970:179016 ISSN：0048-9697
通讯作者：
Bin Zhou<&wdkj&>Fei Yang
作者机构：
[Gui, Ping-Ping] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;[Zeng, Jia-Yue; Liu, Xiao-Ying; Miao, Yu; Li, Cheng-Ru; Zhu, Jin-Qin; Zhang, Min; Liu, A-Xue; Deng, Yan-Ling; Wu, Yang; Liu, Peng-Hui; Li, Yang-Juan; Zeng, Qiang] Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;[Zeng, Jia-Yue; Liu, Xiao-Ying; Miao, Yu; Li, Cheng-Ru; Zhu, Jin-Qin; Zhang, Min; Liu, A-Xue; Deng, Yan-Ling; Wu, Yang; Liu, Peng-Hui; Li, Yang-Juan] Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;[Deng, Yan-Ling] Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA;[Zhang, Min] Department of Environmental Medicine and Climate Science, Icahn School of Medicine at Mount Sinai, New York, NY, USA
通讯机构：
[Bin Zhou] W;[Fei Yang] H;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China
关键词：
Dichloroacetic acid;Disinfection byproducts;Liver function parameters;Trichloroacetic acid
摘要：
Background Toxicological studies have documented that disinfection byproducts (DBPs), the ubiquitous drinking water pollutants, induce hepatotoxicity. Yet epidemiological evidence is sparse.
Toxicological studies have documented that disinfection byproducts (DBPs), the ubiquitous drinking water pollutants, induce hepatotoxicity. Yet epidemiological evidence is sparse.
Objective To assess urinary biomarkers of drinking water DBPs in relation to liver function parameters.
To assess urinary biomarkers of drinking water DBPs in relation to liver function parameters.
Methods We included 1204 reproductive-aged women from the Tongji Reproductive and Environmental (TREE) study in Wuhan, China between December 2018 and July 2021. Urinary trichloroacetic acid (TCAA) and dichloroacetic acid (DCAA) as biomarkers of drinking water DBPs were assessed. Serum liver function parameters such as albumin (ALB), total cholesterol (TC), and alkaline phosphatase (ALP) were determined. Urinary DCAA and TCAA concentrations in relation to liver function parameters were examined by multivariate linear regression or restricted cubic spline (RCS) models.
We included 1204 reproductive-aged women from the Tongji Reproductive and Environmental (TREE) study in Wuhan, China between December 2018 and July 2021. Urinary trichloroacetic acid (TCAA) and dichloroacetic acid (DCAA) as biomarkers of drinking water DBPs were assessed. Serum liver function parameters such as albumin (ALB), total cholesterol (TC), and alkaline phosphatase (ALP) were determined. Urinary DCAA and TCAA concentrations in relation to liver function parameters were examined by multivariate linear regression or restricted cubic spline (RCS) models.
Results There was no evidence of urinary TCAA in relation to serum parameters of liver function. However, monotonic dose-response relationships were estimated between elevated tertiles of urinary DCAA concentrations and increased serum ALP (percent change = 4.25 %; 95 % CI: 0.34 %, 8.32 % for the upper vs. lower tertile) and TC levels (percent change = 3.84 %; 95 % CI: 0.63 %, 7.17 % for the upper vs. lower tertile). These associations remained for urinary DCAA modeled as the continuous exposure variable and were linear in the RCS models. Age, body mass index, and passive smoking status did not modify these associations.
There was no evidence of urinary TCAA in relation to serum parameters of liver function. However, monotonic dose-response relationships were estimated between elevated tertiles of urinary DCAA concentrations and increased serum ALP (percent change = 4.25 %; 95 % CI: 0.34 %, 8.32 % for the upper vs. lower tertile) and TC levels (percent change = 3.84 %; 95 % CI: 0.63 %, 7.17 % for the upper vs. lower tertile). These associations remained for urinary DCAA modeled as the continuous exposure variable and were linear in the RCS models. Age, body mass index, and passive smoking status did not modify these associations.
Conclusion DCAA but not TCAA exposure may contribute to damaged liver function in reproductive-aged women.
DCAA but not TCAA exposure may contribute to damaged liver function in reproductive-aged women.
摘要：
Sensor arrays have been widely used in the identification of metal ions (MIs), whereas traditional sensor arrays often rely on multiple independent sensing elements, which requires higher cost, complicated synthesis, and time-consuming. Herein, we designed a nanozyme sensor array based on a single element with three colorimetric channels for the discrimination of seven MIs (Pb 2+ , Fe 3+ , Zr 4+ , Mg 2+ , Cr 3+ , Ag + , and Hg 2+ ) in environments. Wherein, the Pt/HCNs nanozyme was prepared by integrating hollow carbon nanospheres (HCNs) with platinum nanoparticles, which exhibited enhanced oxidase-like activity and could directly catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to produce three absorption peaks at 370, 450, and 650 nm, providing a single element with three colorimetric channels. Interestingly, due to the interaction of MIs and Pt/HCNs, the MIs differentially regulated the intensity of three absorption peaks of the Pt/HCNs-TMB system, generating distinct colorimetric “fingerprints” that can be further identified through pattern recognition methods. The proposed nanozyme sensor array achieved 100 % accuracy in identifying various MIs and multi-component mixtures, which also showed good performance in the discrimination of MIs in real environmental waters. Thus, our findings provided some new insights for construction of sensor arrays and improving the effective identification of MIs in environments.
Sensor arrays have been widely used in the identification of metal ions (MIs), whereas traditional sensor arrays often rely on multiple independent sensing elements, which requires higher cost, complicated synthesis, and time-consuming. Herein, we designed a nanozyme sensor array based on a single element with three colorimetric channels for the discrimination of seven MIs (Pb 2+ , Fe 3+ , Zr 4+ , Mg 2+ , Cr 3+ , Ag + , and Hg 2+ ) in environments. Wherein, the Pt/HCNs nanozyme was prepared by integrating hollow carbon nanospheres (HCNs) with platinum nanoparticles, which exhibited enhanced oxidase-like activity and could directly catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to produce three absorption peaks at 370, 450, and 650 nm, providing a single element with three colorimetric channels. Interestingly, due to the interaction of MIs and Pt/HCNs, the MIs differentially regulated the intensity of three absorption peaks of the Pt/HCNs-TMB system, generating distinct colorimetric “fingerprints” that can be further identified through pattern recognition methods. The proposed nanozyme sensor array achieved 100 % accuracy in identifying various MIs and multi-component mixtures, which also showed good performance in the discrimination of MIs in real environmental waters. Thus, our findings provided some new insights for construction of sensor arrays and improving the effective identification of MIs in environments.
摘要：
The biodegradation of microcystins is influenced by coexisting pollutants such as micro/nanoplastics, but the short-term effects remain unclear. Herein, we investigated the effects and mechanisms of short-term treatment with polystyrene micro/nanoplastics on the biodegradation of microcystin-LR by Sphingopyxis sp. YF1. Micro/nanoplastics adsorbed microcystin-LR with adsorption efficiencies ranging from 21.36 % to 28.60 % in 15 min. However, subsequent desorption led to decreased adsorption efficiencies at 60 min. Scanning electron microscopy revealed the adhesion between micro/nanoplastics and cells of strain YF1, forming a local microenvironment. At 15 min, the microcystin-LR biodegradation efficiencies in the presence of micro/nanoplastics increased by 21.28 %-49.84 % compared with those in the absence of micro/nanoplastics. The enhancement in biodegradation efficiencies at 15 min may be due to that microcystin-LR was concentrated in the micro/nanoplastics - cells microenvironment, promoting its contact with bacterial degrading enzymes. On the other hand, micro/nanoplastics induced more reactive oxygen species in strain YF1, particularly at 60 min. While higher reactive oxygen species levels inhibited the microcystin-LR biodegradation activity, they may also oxidize microcystin-LR in the late stage. Furthermore, quantitative PCR showed that the expression levels of critical microcystin-LR biodegradation genes, mlrA and PAAase , did not align with the trend of degradation efficiencies. This study represents the first to unveil the temporary effects of micro/nanoplastics on microcystin-LR biodegradation and the underlying mechanisms, and the findings provide insights into the mechanisms involved in the removal of cyanotoxins in complex aquatic environments.
The biodegradation of microcystins is influenced by coexisting pollutants such as micro/nanoplastics, but the short-term effects remain unclear. Herein, we investigated the effects and mechanisms of short-term treatment with polystyrene micro/nanoplastics on the biodegradation of microcystin-LR by Sphingopyxis sp. YF1. Micro/nanoplastics adsorbed microcystin-LR with adsorption efficiencies ranging from 21.36 % to 28.60 % in 15 min. However, subsequent desorption led to decreased adsorption efficiencies at 60 min. Scanning electron microscopy revealed the adhesion between micro/nanoplastics and cells of strain YF1, forming a local microenvironment. At 15 min, the microcystin-LR biodegradation efficiencies in the presence of micro/nanoplastics increased by 21.28 %-49.84 % compared with those in the absence of micro/nanoplastics. The enhancement in biodegradation efficiencies at 15 min may be due to that microcystin-LR was concentrated in the micro/nanoplastics - cells microenvironment, promoting its contact with bacterial degrading enzymes. On the other hand, micro/nanoplastics induced more reactive oxygen species in strain YF1, particularly at 60 min. While higher reactive oxygen species levels inhibited the microcystin-LR biodegradation activity, they may also oxidize microcystin-LR in the late stage. Furthermore, quantitative PCR showed that the expression levels of critical microcystin-LR biodegradation genes, mlrA and PAAase , did not align with the trend of degradation efficiencies. This study represents the first to unveil the temporary effects of micro/nanoplastics on microcystin-LR biodegradation and the underlying mechanisms, and the findings provide insights into the mechanisms involved in the removal of cyanotoxins in complex aquatic environments.
摘要：
To advance the application of Cu-metal–organic framework (Cu-MOF) in uranium wastewater treatment for the nuclear industry, it is essential to address the limitations of its powder form and rigid structure while enhancing its adsorption capacity. In this study, a Cu-MOF@CSTA composite adsorbent was synthesized by solidifying Cu-MOF with chitosan/tannic acid and modifying its functional groups to improve uranium adsorption performance. The U(VI) theoretical maximum adsorption capacity of Cu-MOF@CSTA at 288 K (pH = 5) was 2507.73 mg/g, and the adsorption process was characterized as a spontaneous exothermic reaction. The uranium removal rate in mine wastewater (pore water and seepage water) reached 100 %. Uranium removal efficiencies in wastewater containing 100 mg/L fluoride ions, ammonia ions, and urea were remarkably high at 99.34 %, 99.63 %, and 98.94 %, respectively, demonstrating the composite adsorbent’s robust anti-jamming capability. Mechanistic analysis revealed that the synergistic effects of hydroxyl, amino, and sulfur functional groups on the Cu-MOF@CSTA surface facilitated uranium adsorption. Competitive adsorption experiments confirmed that Cu-MOF@CSTA exhibits excellent selectivity for uranium. The chitosan, solidified by tannic acid as a flexible carrier, stabilized the Cu-MOF, highlighting its significant potential for uranium adsorption in wastewater treatment applications.
To advance the application of Cu-metal–organic framework (Cu-MOF) in uranium wastewater treatment for the nuclear industry, it is essential to address the limitations of its powder form and rigid structure while enhancing its adsorption capacity. In this study, a Cu-MOF@CSTA composite adsorbent was synthesized by solidifying Cu-MOF with chitosan/tannic acid and modifying its functional groups to improve uranium adsorption performance. The U(VI) theoretical maximum adsorption capacity of Cu-MOF@CSTA at 288 K (pH = 5) was 2507.73 mg/g, and the adsorption process was characterized as a spontaneous exothermic reaction. The uranium removal rate in mine wastewater (pore water and seepage water) reached 100 %. Uranium removal efficiencies in wastewater containing 100 mg/L fluoride ions, ammonia ions, and urea were remarkably high at 99.34 %, 99.63 %, and 98.94 %, respectively, demonstrating the composite adsorbent’s robust anti-jamming capability. Mechanistic analysis revealed that the synergistic effects of hydroxyl, amino, and sulfur functional groups on the Cu-MOF@CSTA surface facilitated uranium adsorption. Competitive adsorption experiments confirmed that Cu-MOF@CSTA exhibits excellent selectivity for uranium. The chitosan, solidified by tannic acid as a flexible carrier, stabilized the Cu-MOF, highlighting its significant potential for uranium adsorption in wastewater treatment applications.
摘要：
Microcystin-LR (MC-LR), produced by cyanobacterial harmful algal blooms, poses a serious threat to aquatic ecosystems and human health. Biodegradation is an important method for MC-LR elimination. Aerobic biodegradation has been extensively studied and many bacteria were identified. However, few MC-degrading bacteria have been isolated from anaerobic environments, and these degradation mechanisms are poorly understood. The aim of this study was to collect anaerobic MC-degrading bacteria from lake sediments in Lake Taihu using acclimation culture to assess biodegradation. Five strains with MC-LR degradation ability were isolated, with strain A4 belonging to Citrobacter farmeri (C. farmeri). exhibiting the highest efficiency at 0.486 μg/ml/d. High-performance liquid chromatography (HPLC) identified two novel MC-LR degradation products. Further polymerase chain reaction (PCR) analysis suggested that C. farmeri A4 did not possess the known MC-degrading gene mlrABCD, suggesting the involvement of an mlrABCD-independent anaerobic degradation pathway. Data demonstrated that the bacterial strain C. farmeri A4 found in Lake Taihu exhibited high anaerobic MC-LR degradation properties, which indicated that anaerobic biodegradation may constitute an important biological method for MC-LR removal in natural environment.
摘要：
Microcystin-LR (MC-LR) is a widely distributed cyanotoxin that causes kidney injury. Our previous work identified Sphingopyxis sp. YF1 as a novel bacterial strain capable of efficiently degrading MC-LR, but its protective role and mechanisms against MC-LR-induced kidney injury remain unexplored. In this study, the bioactive metabolite of strain YF1 was identified by mass spectrometry. The beneficial effect of strain YF1 and its bioactive metabolite against MC-LR-induced kidney injury was validated in vivo and in vitro . We found strain YF1 effectively ameliorated MC-LR induced kidney disruption and functional impairment. Mechanistically, strain YF1 significantly improved oxidative stress (SOD, CAT, GSH, and MDA) and inhibited pro-inflammatory factors expression (including IL-6 , IL-18 , IL-1β , and TNF-α ) induced by MC-LR. Notably, strain YF1 not only reduce MC-LR level in mice but also produce a bioactive metabolite astaxanthin. Consistently, both in vivo and in vitro experiments demonstrated that astaxanthin alleviated MC-LR-induced kidney injury by modulating antioxidant pathways and inflammatory cascades. This study suggested that strain YF1 could alleviate MC-LR-induced kidney injury by removing MC-LR and generating astaxanthin that mediated antioxidant and anti-inflammatory effects. These findings highlighted microbial-based antidotes for simultaneously eliminating MC-LR and mitigating its harmful effects.
Microcystin-LR (MC-LR) is a widely distributed cyanotoxin that causes kidney injury. Our previous work identified Sphingopyxis sp. YF1 as a novel bacterial strain capable of efficiently degrading MC-LR, but its protective role and mechanisms against MC-LR-induced kidney injury remain unexplored. In this study, the bioactive metabolite of strain YF1 was identified by mass spectrometry. The beneficial effect of strain YF1 and its bioactive metabolite against MC-LR-induced kidney injury was validated in vivo and in vitro . We found strain YF1 effectively ameliorated MC-LR induced kidney disruption and functional impairment. Mechanistically, strain YF1 significantly improved oxidative stress (SOD, CAT, GSH, and MDA) and inhibited pro-inflammatory factors expression (including IL-6 , IL-18 , IL-1β , and TNF-α ) induced by MC-LR. Notably, strain YF1 not only reduce MC-LR level in mice but also produce a bioactive metabolite astaxanthin. Consistently, both in vivo and in vitro experiments demonstrated that astaxanthin alleviated MC-LR-induced kidney injury by modulating antioxidant pathways and inflammatory cascades. This study suggested that strain YF1 could alleviate MC-LR-induced kidney injury by removing MC-LR and generating astaxanthin that mediated antioxidant and anti-inflammatory effects. These findings highlighted microbial-based antidotes for simultaneously eliminating MC-LR and mitigating its harmful effects.
作者机构：
[Liu, Yu; Li, Le; Liu, Jinquan; Tong, Yuqi; Zhen, Deshuai; Song, Jiayi; Xiong, Lihao; Chen, Sihan] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构：
[Li, L; Zhen, DS ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
关键词：
Fluorescence;Colorimetric detection;Calixarene;Rare-earth ions;Smartphone assisted color detection
摘要：
Anovel fluorescence/colorimetric dual-mode detection strategy for uranium ions (UO(2)(2+)) is presentedbased on a cerium-sulfonyl calix[4]arene (SC4A) platform. The exo- and endo-rim sites of SC4A can coordinate with Ce(3+) and Ce(4+) ions, respectively, quenching Ce(3+) fluorescence and influencing the oxidase-like activity of Ce(4+). In the absence of UO(2)(2+), the solution of 3,3,5,5-tetramethylbenzidine (TMB) remains blue, but upon UO(2)(2+) binding, Ce(3+) dissociates from SC4A, restoring fluorescence, while UO(2)(2+) interacts with oxTMB, turning the solution from blue to colorless. This dual-mode system provides a linear fluorescence detection range of 30-800nM with a detection limit of 20.20nM, and a colorimetric range of 30-800nM with a detection limit of 27.78nM. By combining high-sensitivity fluorescence with visual colorimetric analysis, the proposed method possesses high sensitivity, accuracy, and reliability. Notably, smartphone-based color capture facilitates rapid and convenient sample analysis, enabling straightforward quantification at varying UO(2)(2+) concentrations. The method has been successfully applied to real water and urine samples, demonstrating its practical utility in environmental and biological monitoring of UO(2)(2+).
作者机构：
[Liu, Hangxi; Luo, Gemiao; Deng, Zhongliang; Zou, Lintao; Wen, Piaoting; Zhou, Xuan] Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China. dzl021015@163.com;[Tang, Honghua] The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, China;[Yang, Ruifu] State Key Laboratory of Pathogen and Biosecruity, Academy of Military Medical Sciences, Beijing, China
通讯机构：
[Zhongliang Deng] D;Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China
摘要：
Hepatitis B virus (HBV) is a major pathogen posing significant challenges to global public health, making early diagnosis critical for preventing severe liver diseases. We previously developed a fluorescent biosensor named PAM-dependent dsDNA Target-activated Cas12f1 Trans Reporter (PDTCTR). However, its reliance on specialized fluorescence equipment and lack of visual readout limited its application in resource-limited settings. To address these limitations, we developed a lateral flow assay (LFA) that integrates enzymatic recombinase amplification (ERA) with the Cas12f1_ge4.1 system. This approach enables the specific amplification of the HBV target gene through ERA and leverages the precise cleavage activity of Cas12f1_ge4.1 for enhanced signal amplification. The entire detection process is completed within 50 minutes, with results readily interpretable through visual inspection. The assay achieves a minimum detection limit of 100 copies per μL and demonstrates high specificity, showing no cross-reactivity with related viruses. In a validation study involving 71 clinical samples, the system achieved a sensitivity of 94.23%, specificity of 100%, and a kappa value of 0.90 compared to quantitative PCR (qPCR), indicating high reliability. This method thus shows promise as an effective tool for early HBV diagnosis, particularly suited for rapid, on-site detection in resource-constrained environments, and holds broad potential for diverse applications.
期刊：
Ecotoxicology and Environmental Safety,2025年290:117580 ISSN：0147-6513
通讯作者：
Cao, Y;Zhou, ZZ
作者机构：
[Cao, Yi; Li, Kuanhang] Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Zhou, Zhengzheng] Southern Med Univ, NMPA Key Lab Safety Evaluat Cosmet, Guangdong Prov Key Lab Trop Dis Res, Dept Hyg Inspection & Quarantine Sci,Sch Publ Hlth, Guangzhou 510515, Guangdong, Peoples R China.
通讯机构：
[Cao, Y ] U;[Zhou, ZZ ] S;Univ South China, Hengyang Med Sch, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;Southern Med Univ, NMPA Key Lab Safety Evaluat Cosmet, Guangdong Prov Key Lab Trop Dis Res, Dept Hyg Inspection & Quarantine Sci,Sch Publ Hlth, Guangzhou 510515, Guangdong, Peoples R China.
关键词：
Autophagy;Lipid profiles;Oral toxicity;SiO(2) nanoparticles (NPs);Trace element balance
摘要：
Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influences of orally exposed SiO2 NPs on lipid profiles in gut-liver axis. To this end, we treated mice with 16, 160 or 1600 mg/kg bodyweight SiO2 NPs via intragastric route. After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. Consistently, lipidomics results showed that most of the lipid classes in mouse intestines and livers were decreased following SiO2 NP administration. We further identified the lipid classes significantly decreased in both intestines and livers, such as phosphatidylserine (PS), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Only a few lipid classes, such as anandamide, showed opposite trends in these organs. For metabolism pathway, SiO2 NPs suppressed autophagy, showing as a significant decrease of microtubule-associated protein 1 A/1B light chain 3 (LC3) and adipose triglyceride lipase (Atgl), accompanying with an accumulation of P62, in both intestines and livers. However, lysosomal-associated membrane protein 2 (Lamp2) showed different trend, that it was significantly increased in intestines but decreased in livers. Combined, our results indicated that intragastric administration of SiO2 NPs altered trace element balance and lipid profiles, accompanying with a change of autophagic lipolysis proteins, in mouse gut-liver axis.
Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influences of orally exposed SiO2 NPs on lipid profiles in gut-liver axis. To this end, we treated mice with 16, 160 or 1600 mg/kg bodyweight SiO2 NPs via intragastric route. After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. Consistently, lipidomics results showed that most of the lipid classes in mouse intestines and livers were decreased following SiO2 NP administration. We further identified the lipid classes significantly decreased in both intestines and livers, such as phosphatidylserine (PS), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Only a few lipid classes, such as anandamide, showed opposite trends in these organs. For metabolism pathway, SiO2 NPs suppressed autophagy, showing as a significant decrease of microtubule-associated protein 1 A/1B light chain 3 (LC3) and adipose triglyceride lipase (Atgl), accompanying with an accumulation of P62, in both intestines and livers. However, lysosomal-associated membrane protein 2 (Lamp2) showed different trend, that it was significantly increased in intestines but decreased in livers. Combined, our results indicated that intragastric administration of SiO2 NPs altered trace element balance and lipid profiles, accompanying with a change of autophagic lipolysis proteins, in mouse gut-liver axis.
摘要：
Exposure to microcysatin-LR (MC-LR) is known to result in kidney damage, however the underlying mechanisms involved in MC-LR-initiated renal injury are not known. Thus, the aim of this study was to examine the effects of exposure to MC-LR on human embryo kidney (HEK 293) cell in vitro and male C57BL/6 in vivo. In the in vitro study, HEK 293 cells were incubated with MC-LR (20 mu M) for 24 hr. Treatment with MC-LR significantly increased the protein expression of RAF and ERK as well as mRNA expression levels of inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta. These findings were confirmed when HEK 293 cells were co-incubated with ERK inhibitor U0126 and MC-LR demonstrating a decrease in protein expression of RAF, ERK, and mRNA levels of pro-inflammatory cytokines. Male C57BL/6 mice were intraperitoneally (ip) injected with MC-LR (20 mu g/kg) daily for 21 days. Histopathological analysis demonstrated significant glomerular and tubular damage with inflammatory infiltration. The expression levels of pro-inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta were significantly elevated following MC-LR treatment. Administration of MC-LR asignificantly enhanced the protein phosphorylation levels of RAF and ERK. Data demonstrated that exposure to MC-LR induced morphological tissue damage and renal inflammatory reactions by activating the RAF/ERK signaling pathway.
期刊：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES,2025年88(7):291-300 ISSN：1528-7394
通讯作者：
Yang, Yue;Yang, F;Yang, Y
作者机构：
[Long, Sihong; Yang, F; Yang, Yue; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Wen, Cong] Changsha Yuhua Dist Ctr Dis Control & Prevent, Changsha, Peoples R China.;[Yang, Yue; Zeng, Wen] Cent Hosp Shaoyang, Dept Publ Hlth, Shaoyang, Peoples R China.
通讯机构：
[Yang, F ; Yang, Y] U;[Yang, Y ] C;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;Cent Hosp Shaoyang, Dept Publ Hlth, Shaoyang, Peoples R China.
关键词：
Microcystin-LR;apoptosis;jejunum;RAF/ERK
摘要：
Microcystin-LR (MC-LR), a class of cyclic heptapeptide compounds synthesized by cyanobacterial species, presents a significant risk to ecological systems and public health. Exposure to MC-LR was found to induce damage to various organs. One of the target organ systems affected by MC-LR is the gastrointestinal tract (GIT). However, the majority of studies regarding GIT focused on colorectal toxicity, with little attention paid to small intestinal toxic injuries, in particular jejunum. Thus, the aim of this study was to investigate the effects attributed to MC-LR exposure on apoptosis and underlying mechanisms utilizing a mouse jejunum injury model following chronic low-dose MC-LR treatment. A total of 40 C57BL/6 male mice were randomly divided into 4 groups with each group receiving drinking water containing 0, 1, 60, or 120 µg/L MC-LR for a duration of 12 months. Results indicated that exposure to MC-LR induced pathological alterations in jejunal tissue as evidenced by abnormal villous serration, crypt disorganization, and lymphocyte infiltration. TUNEL assays demonstrated a significant increase in apoptotic cell count in the 60 and 120 µg/L groups. The 60 and 120 µg/L MC-LR treatment groups exhibited elevated mRNA expression of Bax accompanied by significant reduction in mRNA expression of Bcl-2. The protein levels of cleaved caspase-3 were markedly elevated in the 60 and 120 µg/L MC-LR groups. The protein expression levels of p-RAF and p-ERK were significantly increased in the 60 and 120 µg/L MC-LR treatment groups. Data demonstrated suggest that the RAF/ERK signaling pathway may be involved in MC-LR- induced jejunal apoptosis.
摘要：
Beryllium and its compounds are classified as carcinogens, and prolonged exposure can trigger chronic beryllium disease. The Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling is known to play a critical role in the development and progression of numerous diseases. Leukemia inhibitory factor (LIF), a key upstream cytokine of the JAK-STAT pathway, has been implicated in regulating inflammatory responses and epithelial-mesenchymal transition (EMT) in various diseases. However, the specific involvement of the JAK-STAT pathway and LIF in beryllium sulfate (BeSO₄)-induced EMT in human bronchial epithelial (16HBE) cells remains unclear. To investigate the regulatory mechanisms, we examined the effects of BeSO₄ on 16HBE cells and targeted the JAK-STAT pathway using both pharmacological inhibition (niclosamide) and genetic silencing of LIF. Subsequently, we assessed cell morphology, proliferative capacity, inflammatory protein levels, and EMT marker expression. Our findings demonstrated that BeSO₄ exposure inhibited 16HBE cell proliferation and activated the JAK-STAT pathway. Pretreatment with niclosamide significantly mitigated cellular inflammation and the EMT process induced by BeSO₄. Additionally, silencing LIF markedly reduced JAK-STAT pathway activation and decreased the expression of EMT markers. This study uncovers a novel mechanism underlying BeSO₄-induced EMT in 16HBE cells, providing valuable insights into the molecular mechanisms of toxicity induced by beryllium and its compounds.
作者：
Bu, Zhijian;Liu, Jinjin*;Tang, Zheng;Liang, Hao;Bai, Qinqin;...
期刊：
Chemical Communications,2025年61(42):7672-7675 ISSN：1359-7345
通讯作者：
Liu, Jinjin;Niu, XH
作者机构：
[Bu, Zhijian; Liu, Jinjin; Bai, Qinqin; Tang, Zheng; Liu, JJ; Liang, Hao; Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Liu, Shuangquan; Tang, Zheng; Niu, Xiangheng] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Liu, JJ; Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang 421001, Peoples R China.
摘要：
Different from crystalline cobalt oxides (Co(3)O(4) and CoO) and most reported nanozymes, amorphous CoO(x) was found to exhibit better oxidase-like catalytic performance than the peroxidase one. Mechanistic investigations revealed that the introduction of H(2)O(2) could decompose CoO(x) into inactive Co(2+) under acidic conditions, leading to the loss of catalytic activity. With the unusual phenomenon, a proof-of-concept "turn-off" cascade system was fabricated to detect glucose colorimetrically via combining CoO(x) with glucose oxidase.
摘要：
Since the revelation of Fe 3 O 4 nanoparticles with enzyme-like characteristics, nanozymes present a promising alternative to traditional bio-enzymes due to their straightforward preparation, good robustness, and adjustable performance. In addition to enzyme-mimetic catalysis, these materials also tend to show optical, electrical, thermal, and magnetic properties. Integrating nanozyme catalysis with these properties into an entity can offer fascinating merits to biochemical sensing. Especially, fluorescent nanozymes, a class of materials combining enzyme-mimicking activity and fluorescence, have been drawing growing interest in the analytical chemistry community in the past five years. To highlight the achievements and progress made in this emerging area, here we, for the first time, present a comprehensive summary on fluorescent nanozymes and their roles in advancing analytical detection. First, a definition of fluorescent nanozymes is given, and their bifunctional features are discussed. Then, common strategies employed to develop different material types of fluorescent nanozymes are introduced. Emphatically, their unique contributions to biochemical analysis are clarified in detail, along with various applications in environmental monitoring, food safety analysis, and biomedical detection. Finally, substantial potential of fluorescent nanozymes in realizing advanced detection as well as existing challenges is spotlighted. Our review provides a comprehensive and up-to-date reference on fluorescent nanozymes, which will attract more attention and guide future efforts to further promote the promising field.
Since the revelation of Fe 3 O 4 nanoparticles with enzyme-like characteristics, nanozymes present a promising alternative to traditional bio-enzymes due to their straightforward preparation, good robustness, and adjustable performance. In addition to enzyme-mimetic catalysis, these materials also tend to show optical, electrical, thermal, and magnetic properties. Integrating nanozyme catalysis with these properties into an entity can offer fascinating merits to biochemical sensing. Especially, fluorescent nanozymes, a class of materials combining enzyme-mimicking activity and fluorescence, have been drawing growing interest in the analytical chemistry community in the past five years. To highlight the achievements and progress made in this emerging area, here we, for the first time, present a comprehensive summary on fluorescent nanozymes and their roles in advancing analytical detection. First, a definition of fluorescent nanozymes is given, and their bifunctional features are discussed. Then, common strategies employed to develop different material types of fluorescent nanozymes are introduced. Emphatically, their unique contributions to biochemical analysis are clarified in detail, along with various applications in environmental monitoring, food safety analysis, and biomedical detection. Finally, substantial potential of fluorescent nanozymes in realizing advanced detection as well as existing challenges is spotlighted. Our review provides a comprehensive and up-to-date reference on fluorescent nanozymes, which will attract more attention and guide future efforts to further promote the promising field.
作者机构：
[Shuchen Liu; Xiaoxia Zhu; Zhuona Wu; Runtian Li; Zhiyun Meng; Ruolan Gu; Hui Gan] Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China;[Lintao Li] School of Public Health, University of South China, Hengyang 421001, China;Author to whom correspondence should be addressed.;[Haiyang Wu] Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China<&wdkj&>School of Public Health, University of South China, Hengyang 421001, China;[Guifang Dou] Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China<&wdkj&>Author to whom correspondence should be addressed.
通讯机构：
[Guifang Dou] B;Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Radiation-induced intestinal injury (RIII) is a significant concern for cancer patients receiving radiation therapy, as it can lead to complications such as radiation enteropathy. Presently, there are limited options for preventing or treating RIII. Tea polyphenols (TP), found in tea, provide various health benefits, but their antiradiation mechanisms are not fully understood. C57BL/6 mice pre-treated with TP for five days showed a significant improvement in survival rates after being exposed to 10 Gy of 60Co radiation. In the same way, abdominal exposure to 15 Gy of 60Co radiation effectively mitigated radiation-induced colon shortening, damage to intestinal tissues, oxidative stress, the release of inflammatory factors, and disruptions in intestinal microbial balance. In addition, TP treatment lowered the elevation of reactive oxygen species (ROS), iron imbalance, mitochondrial damage, and ferroptosis in IEC-6 cells post-irradiation. Utilizing network pharmacology, molecular docking, and affinity testing, we identified that TP has the capability to target the Nrf2/HO-1/GPX4 signaling pathway, while EGCG, a principal constituent of TP, interacts with HSP90 and mitigates radiation-induced ferroptosis. These findings suggest that TP may serve as a promising therapeutic agent to alleviate radiation-induced intestinal injury (RII).
作者机构：
[Li, Yuan; Liu, Xiaocheng; Rang, Ouyan; Liu, Xu; Li, Guojuan; Wang, Mu; Yang, Qing] Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch,Dept Basic Med, Hengyang 421001, Hunan, Peoples R China.;[Chen, Junmin] Univ South China, Affiliated Nanhua Hosp, Hlth Sch Nucl Ind, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Liu, Xu] Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Rang, O; Wang, M ] U;Univ South China, Affiliated Nanhua Hosp, Clin Mass Spectrometry Lab,Clin Res Inst, Hengyang Med Sch,Nucl Ind Hyg Sch,Dept Basic Med, Hengyang 421001, Hunan, Peoples R China.
摘要：
Bisphenol AF (BPAF), a fluorinated alternative to the plasticizer bisphenol A (BPA), is found in both the environment and the human body. Fructose is one of the sweeteners that has been widely used in recent years. Prior research has verified that the combined exposure to fructose and BPA considerably worsened the impact on glycolipid metabolism. However, it is currently unclear whether BPAF have a combination effect on health with fructose. Serum glucose and insulin, liver biochemistry, histology of the liver and adipose tissue, serum profiles of amino acids, vitamins, bile acids, steroid hormones, catecholamines, and adipocytokines like leptin, omentin-1, adiponectin, asprosin, and adipocyte fatty acid binding protein (A-FABP) of male mice were all investigated in this study following a week of combined exposure to two doses of BPAF (lower dose: 0.25, and higher dose: 25μg/kg daily). The results showed that simultaneous exposure to lower doses of BPAF and fructose considerably increased blood insulin and liver coefficient, total bilirubin, direct bilirubin, and glucose while significantly decreasing body weight, food intake, liver creatinine, and serum leptin, asprosin, and A-FABP. According to histology analysis, adipocyte enlargement may result from lower dose BPAF and fructose combined exposure, while bile duct dilatation may result from both lower and higher doses of BPAF combined with fructose. Concurrently, the combination of lower doses of BPAF and fructose increased the release of adrenocortical hormones and catecholamines, worsened metabolic disorders in amino acids such as histidine, arginine and proline, branched chain amino acid (isoleucine), and aromatic amino acids (tryptophan and phenylalanine), and aggravated the depletion of vitamin B12 and A. Interestingly, following the combined exposure to BPAF and fructose, bile acids including taurocholic acid, deoxycholic acid, cholic acid, and taurine ursodeoxycholic acid rose in a dose-dependent manner. According to these results, exposure to fructose and BPAF together may have a more detrimental effect on metabolism than either substance alone. Further research should be conducted to verify the impact of joint exposure to BPAF and fructose on human.
摘要：
The success of graphene oxides has gained extensive research interests in developing novel 2D nanomaterials (NMs). WS(2) nanosheets (NSs) are novel transition metal-based 2D NMs, but their toxicity is unclear. In this study, we investigated the oral toxicity of WS(2) NSs to mouse intestines. Male mice were administrated with vehicles, 1, 10, or 100 mg/kg NSs via intragastric route, once a day, for 5days. The results indicate that the NSs did not induce pathological or ultrastructural changes in intestines. There were minimal changes of trace elements that the exposure did not induce W accumulation, and only Co levels were dose-dependently increased. Lipid droplets were observed in all groups of mice, but lipidomics data indicate that WS(2) NSs only significantly decreased four lipid species, all belonging to phosphatidylcholine (PC). The levels of proteins regulating autophagic lipolysis, namely, LC3, lysosomal associated membrane protein 2 (LAMP2) and perilipin 2 (PLIN2), were increased, but it was only statistically significantly different for LC3. The results of this study suggest that repeated intragastric exposure to WS(2) NSs only induced minimal influences on pathological injury, trace element balance, autophagy, and lipid profiles in mouse intestines, indicating relatively high biocompatibility of WS(2) NSs to mouse intestine via oral route.
期刊：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES,2025年88(9):364-372 ISSN：1528-7394
通讯作者：
Yang, Yue;Yang, F;Yang, Y
作者机构：
[Yang, F; Yang, Yue; Yang, Fei; Yang, Mingjie; Zhan, Chunhua] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.;[Zheng, Shuilin] Changsha Ctr Dis Control & Prevent, Changsha, Peoples R China.;[Yang, Yue; Zeng, Wen] Cent Hosp Shaoyang, Dept Publ Hlth, Shaoyang, Peoples R China.;[Yang, F; Yang, Fei] Univ South China, Nanhua Hosp, Hengyang Med Sch, Nucl Med Dept, Hengyang, Peoples R China.
通讯机构：
[Yang, F ; Yang, Y] U;[Yang, Y ] C;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Peoples R China.;Cent Hosp Shaoyang, Dept Publ Hlth, Shaoyang, Peoples R China.;Univ South China, Nanhua Hosp, Hengyang Med Sch, Nucl Med Dept, Hengyang, Peoples R China.
摘要：
The global phenomenon of cyanobacterial bloom pollution is spreading globally due to climate change and eutrophication. It is well established that harmful cyanobacteria produce a wide range of toxins including microcystin-LR (MC-LR), a cyclic heptapeptide toxin known to damage various organs. The intestinal tract is the main site of MC-LR absorption and one of the targets susceptible to toxicity. Currently, studies on the enterotoxic effects of MC-LR predominantly focused on the colorectum, with limited investigations addressing the impact of microcystins on the small intestine. Therefore, the aim of our study was to examine the impact of chronic 9-month exposure of mice to low-dose 120 μg/L MC-LR in drinking water on ileal inflammation and potential mechanisms underlying these effects. Our findings showed that in mice chronically administered with low-dose MC-LR disorganized intestinal epithelial cells, lymphocytic infiltration and disturbed crypt arrangement were detected. The results of qPCR and Western blot demonstrated that, in comparison to control, the mRNA expression levels of pro-inflammatory factors IL-6, IL-17, IL-18, and IFN-γ were markedly elevated in the ileal tissue of mice treated with MC-LR, associated with significant increases in protein expression levels of p-PI3K, p-AKT, and p-mTOR. Taken together, evidence indicates that MC-LR induces ileal inflammation and histopathological damage involved activation of the PI3K/AKT/mTOR signaling pathway.
