期刊：
Journal of Inflammation Research,2025年18:5727-5739 ISSN：1178-7031
通讯作者：
Zhongliang Deng<&wdkj&>Jun He
作者机构：
[Daoyong Liao; Ying Huang; Chao Li; Yuan Li] The Affiliated Nanhua Hospital, Department of Clinical Laboratory, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China;[Zhongliang Deng] Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, People’s Republic of China;[Xue Yang; Jun He] The Affiliated Nanhua Hospital, Department of Clinical Laboratory, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China<&wdkj&>Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, People’s Republic of China
通讯机构：
[Zhongliang Deng] D;[Jun He] T;Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, People’s Republic of China<&wdkj&>The Affiliated Nanhua Hospital, Department of Clinical Laboratory, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China<&wdkj&>Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, People’s Republic of China
摘要：
PURPOSE: Mycoplasma pneumoniae, a clinically significant respiratory pathogen, primarily causes community-acquired pneumonia and contributes to asthma development, with its persistent infection frequently resulting in fibrotic pulmonary changes and structural airway abnormalities. This study investigates the signaling pathways regulating CCL20 expression in THP-1 cells following M. pneumoniae infection and its impact on cell migration and epithelial-mesenchymal transition (EMT). METHODS: THP-1 cells were infected with M. pneumoniae, and the expression of CCL20 was measured over time and at various doses. In addition, co-culture experiments were performed using M. pneumoniae-infected THP-1 cells and bronchial epithelial cells to assess EMT and cell migration. RESULTS: M. pneumoniae infection significantly upregulated CCL20 production in THP-1 cells via the AKT-ERK1/2-AP1 pathway, a process that was both time- and dose-dependent. Furthermore, co-culturing M. pneumoniae-infected THP-1 cells with 16HBE cells promoted EMT and increased cell migration, a process that is believed to be associated with CCL20. CONCLUSION: This study provides insights into the molecular mechanisms linking CCL20 to cell migration, highlighting potential therapeutic targets for M. pneumoniae-related lung diseases.
作者机构：
[Yuting Li; Xiayu Zhao; Yichun Hu; Siran Li; Huidi Zhang; Yang Cao; Rui Wang; Lichen Yang] Key Laboratory of Public Nutrition and Health, National Health Commission of the People's Republic of China, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 241001, Hunan, China;[Xiaoyun Shan] Key Laboratory of Public Nutrition and Health, National Health Commission of the People's Republic of China, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China<&wdkj&>Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 241001, Hunan, China
关键词：
25-hydroxyvitamin D;Gene polymorphism;Osteocalcin;Procollagen type 1 N-terminal propeptide;β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide
摘要：
Objective This study aimed to investigate possible serum 25-hydroxyvitamin D [25(OH)D] cutoffs for the associations between 25(OH)D and Bone turnover markers (BTMs), and how GC gene variation influences such cutoffs in Chinese women of childbearing age.
This study aimed to investigate possible serum 25-hydroxyvitamin D [25(OH)D] cutoffs for the associations between 25(OH)D and Bone turnover markers (BTMs), and how GC gene variation influences such cutoffs in Chinese women of childbearing age.
Methods In total, 1,505 non-pregnant or non-lactating women (18–45 years) were recruited from the 2015 Chinese Adult Chronic Disease and Nutrition Surveillance. Serum 25(OH)D, osteocalcin (OC), procollagen type 1 N-terminal propeptide (P1NP), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), and single nucleotide polymorphisms were determined. Locally weighted regression and smoothing scatterplot and segmented regression were performed to estimate the 25(OH)D thresholds.
In total, 1,505 non-pregnant or non-lactating women (18–45 years) were recruited from the 2015 Chinese Adult Chronic Disease and Nutrition Surveillance. Serum 25(OH)D, osteocalcin (OC), procollagen type 1 N-terminal propeptide (P1NP), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), and single nucleotide polymorphisms were determined. Locally weighted regression and smoothing scatterplot and segmented regression were performed to estimate the 25(OH)D thresholds.
Results The median serum 25(OH)D was 16.63 (11.96–22.55) ng/mL and the prevalence of low serum 25(OH)D (< 12 ng/mL) was 25.2%. Women with the lowest 25(OH)D had the highest β-CTX. After adjustment for the confounders, 25(OH)D cutoffs for OC [14.04 (12.84–15.23) ng/mL], β-CTX [13.94 (12.49–15.39) ng/mL], and P1NP [13.87 (12.37–15.37) ng/mL] in the whole population, cutoffs for OC [12.30 (10.68–13.91) ng/mL], β-CTX [12.23 (10.22–14.23) ng/mL], and P1NP [11.85 (10.40–13.31) ng/mL] in women with the GC rs2282679 G allele, and cutoffs for OC [12.75 (11.81–13.68) ng/mL], β-CTX [13.05 (11.78–14.32) ng/mL], and P1NP [12.81 (11.57–14.06) ng/mL] in women with the GC rs2282679 T allele, were observed. Below these cutoffs, BTMs were negatively associated with 25(OH)D, while above these cutoffs, BTMs plateaued.
The median serum 25(OH)D was 16.63 (11.96–22.55) ng/mL and the prevalence of low serum 25(OH)D (< 12 ng/mL) was 25.2%. Women with the lowest 25(OH)D had the highest β-CTX. After adjustment for the confounders, 25(OH)D cutoffs for OC [14.04 (12.84–15.23) ng/mL], β-CTX [13.94 (12.49–15.39) ng/mL], and P1NP [13.87 (12.37–15.37) ng/mL] in the whole population, cutoffs for OC [12.30 (10.68–13.91) ng/mL], β-CTX [12.23 (10.22–14.23) ng/mL], and P1NP [11.85 (10.40–13.31) ng/mL] in women with the GC rs2282679 G allele, and cutoffs for OC [12.75 (11.81–13.68) ng/mL], β-CTX [13.05 (11.78–14.32) ng/mL], and P1NP [12.81 (11.57–14.06) ng/mL] in women with the GC rs2282679 T allele, were observed. Below these cutoffs, BTMs were negatively associated with 25(OH)D, while above these cutoffs, BTMs plateaued.
Conclusion In Chinese women of childbearing age, there were thresholds effect of serum 25(OH)D concentrations on BTMs. The results indicated that serum 25(OH)D concentrations < 13.87 ng/mL in this population had adverse influences on maintaining bone remodeling. BTMs were suppressed at a relatively lower serum 25(OH)D in women with the GC rs2282679 G allele compared with those with the T allele.
In Chinese women of childbearing age, there were thresholds effect of serum 25(OH)D concentrations on BTMs. The results indicated that serum 25(OH)D concentrations < 13.87 ng/mL in this population had adverse influences on maintaining bone remodeling. BTMs were suppressed at a relatively lower serum 25(OH)D in women with the GC rs2282679 G allele compared with those with the T allele.
作者机构：
[Tian, Qingzhen; Liu, Jinjin; Tang, Zheng; Niu, Xiangheng; Li, Shu; Chen, Xinyu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.
摘要：
Organophosphorus pesticides (OPs) are widely used in agricultural production, posing a great threat to human health and the environment. Given that different OPs present different toxicology and toxicities, identifying individual pesticide residues becomes important for assessing food safety and environmental implications. In this work, a kinetics difference-driven analyte hydrolysis strategy is proposed for the first time and validated to identify p-nitrophenyl pesticides by developing an organophosphorus hydrolase-like nanozyme-coded sensor array. Ultrasmall bare CeO(2) nanoparticles were synthesized and employed as the only sensing unit to catalyze the hydrolysis of multiple analytes. With catalytic preferences and kinetics differences under identical reaction conditions, five common OPs analogues (methyl-paraoxon, paraoxon, methyl-parathion, parathion, and fenitrothion) offered discriminable colors. By coupling the color fingerprints with pattern recognition, the accurate identification of individual p-nitrophenyl pesticides and their mixtures at a variety of concentrations and ratios was verified in laboratory and practical scenarios. Attractively, apart from excellent performance and convenient operation, the proposed hydrolytic nanozyme-coded pattern presents strong resistance against redox substances that often cause interference in previous oxidoreductase-based sensor arrays. Our study provides a new paradigm of discriminating specific OPs precisely, showing promising applications in multitarget analysis in complex matrices.
作者机构：
[Peng, Jiayi; Liu, Jinjin; Tang, Zheng; Liu, JJ; Liang, Hao; Niu, Xiangheng; Chen, Xinyu] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Liu, Shuangquan; Tang, Zheng; Niu, Xiangheng] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Liu, JJ; Niu, XH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang 421001, Peoples R China.
摘要：
With their important role in regulating intracellular redox balance and maintaining cell homeostasis, endogenous mercaptans are recognized as biomarkers of many diseases in clinical practice, and thus establishing efficient yet simple methods to distinguish and quantify endogenous mercaptans is of great significance for health management. Here, we propose a machine learning-enabled time-resolved nanozyme-encoded strategy to identify endogenous mercaptans in the presence of potential interferents for disease diagnosis. Diethylenetriaminepenta(methylenephosphonic) acid was first employed to coordinate with Mn(3+) to prepare a new amorphous nanozyme, which exhibited excellent oxidase-like activity in catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine to its blue oxide. The addition of endogenous mercaptans (cysteine, homocysteine, and glutathione) could competitively suppress the chromogenic process to different extents due to their discrepant antioxidant abilities, providing specific fingerprints over time for each species. With this mechanism, a time-resolved sensor array with the nanozyme as a sole sensing unit was constructed to accurately identify different types and levels of mercaptans and their various mixtures with the help of pattern recognition. Furthermore, machine learning was combined with the sensor array to construct a stepwise prediction model consisting of concentration-independent classification and concentration-associated regression, which could not only differentiate cancer cells from normal ones based on intracellular glutathione but also evaluate the severity of cardiovascular diseases according to serum homocysteine, showing great application potential in disease diagnosis.
摘要：
BACKGROUND: Coinfections involving multiple diarrheal viruses have gained increasing recognition as a significant cause of acute gastroenteritis in recent years. Understanding the genetic diversity and evolutionary relationships of these viruses is crucial for effective outbreak identification and tracking. OBJECTIVE: To report two cases of HAdV and SaV coinfections and elucidate the genetic diversity and evolutionary patterns of these viruses through whole-genome sequencing (WGS) and phylogenetic analysis. METHODS: A total of 873 diarrheal stool samples were collected from sentinel hospitals in Shenzhen, China, in 2021. The collected stool samples were identified using RT-PCR and positive samples were subjected to WGS on the NovaSeq platform. phylogenetic trees were constructed using MEGA to analyze genetic relationships. RESULTS: The sequencing results showed that both samples were human adenovirus type 41, which clustered in two distinct evolutionary clades. Additionally, we also retrieved the complete genome of sapovirus (GI.1 genotype) from the same sample. Phylogenetic analysis revealed that they were similar to previously reported strains, belonging to the clade predominating in China. CONCLUSIONS: This study reveals the genetic diversity of epidemic strains involved in coinfections of human adenovirus and sapovirus. The findings establish a groundwork for the identification and traces of acute gastroenteritis outbreaks.
摘要：
Inflammatory bowel disease (IBD) is a complex gastrointestinal disorder attributed to genetic and environmental factors. Microcystin-leucine-arginine (MC-LR) is an environmental toxin that accumulates in the gut and produces intestinal damage. The aim of this study was to investigate the effects of exposure to MC-LR on development and progression of IBD as well examine the underlying mechanisms of microcystin-initiated tissue damage. Male C57BL/6 mice were treated with either MC-LR alone or concurrently with dextran-sulfate sodium (DSS). Mice were divided into 4 groups (1): PBS gavage (control, CT) (2); 200μg/kg MC-LR gavage (MC-LR) (3); 3% DSS Drinking Water (DSS); and (4) 3% DSS Drinking Water + 200μg/kg MC-LR gavage (DSS + MC-LR). The mice in each experimental group exhibited reduced body weight, shortened colon length, increased disease activity index (DAI) score, a disrupted intestinal barrier, and elevated levels of proinflammatory cytokines compared to control. Compared to the group treated with MC-LR alone, colitis symptoms were exacerbated following combined exposure to both DSS and MC-LR. Subsequent experiments confirmed that MC-LR or DSS increased protein phosphorylation levels of Janus Kinase1 (JAK1) and Signal Transducer and Activator of Transcription3 (STAT3). Compared to group treated with MC-LR alone, the combined treatment of DSS and MC-LR also significantly upregulated the expression of related proteins. In conclusion, our study indicates that MC-LR-induced colitis involves activation of JAK1/STAT3 signaling pathway and that MC-LR exacerbates DSS-induced colitis through the same pathway.
摘要：
NAD(P)H dehydrogenase quinone 1 (NQO1) is overexpressed in various cancers and is strongly associated with an immunosuppressive microenvironment and poor prognosis. In this study, we explored the role of NQO1 in the microenvironment, prognosis and immunotherapy of Hepatocellular carcinoma (HCC) using multi-omics analysis and machine learning. The results revealed that NQO1 was significantly overexpressed in HCC cells. NQO1(+)HCC cells were correlated with poor prognosis and facilitated tumor-associated macrophages (TAMs) polarization to M2 macrophages. We identified core NQO1-related genes (NRGs) and developed the NRGs-related risk-scores in hepatocellular carcinoma (NRSHC). The comprehensive nomogram integrating NRSHC, age, and pathological tumor-node-metastasis (pTNM) Stage achieved an area under the curve (AUC) above 0.7, demonstrating its accuracy in predicting survival outcomes and immunotherapy responses of HCC patients. High-risk patients exhibited worse prognoses but greater sensitivity to immunotherapy. Additionally, a web-based prediction tool was designed to enhance clinical utility. In conclusion, NQO1 may play a critical role in M2 polarization and accelerates HCC progression. The NRSHC model and accompanying tools offer valuable insights for personalized HCC treatment.
期刊：
Colloids and Surfaces B: Biointerfaces,2025年254:114797 ISSN：0927-7765
通讯作者：
Xiao, Fubing
作者机构：
[Yang, Shengyuan; Wang, Yuxiao; Ding, Ying; Deng, Chenyi; Huang, Sijia; Huang, Haibo] Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[Chen, Shuangxi] The First Affiliated Hospital, Department of Neurology, Multi-Omics Research Center for Brain Disorders, Hengyang Medical School, University of South China, Hengyang 421001, China;[Xiao, Fubing] Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China. Electronic address: xiaofubing2008@163.com
通讯机构：
[Xiao, Fubing] H;Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China. Electronic address:
关键词：
Gelatin-curcumin nanoparticles;Hydrogel microneedles;Infected wound healing;Inflammation modulation;MMP-responsive drug delivery
摘要：
Effective management of bacterial infection and inflammatory responses is critical for accelerating infected wound healing. Herein, we developed a multifunctional hydrogel microneedle system (GMPL@GC MNs) integrating gelatin methacryloyl (GM) as the matrix material, ε-poly-L-lysine (PL) for enhanced antibacterial properties, and gelatin-curcumin nanoparticles (GC NPs) for inflammation modulation. The system exploits elevated matrix metalloproteinase (MMP) levels at wound sites to trigger specific degradation of GC NPs and controlled curcumin (Cur) release. This MMP-responsive mechanism enables reactive oxygen species (ROS) scavenging, inflammatory response suppression, and sustained drug delivery over 96 h. In bacteria-infected wound models, the GMPL@GC MNs demonstrated excellent therapeutic efficacy, achieving 99.1% wound closure rate within 10 days through combined antibacterial, antioxidant, and anti-inflammatory actions. The system's multifunctionality and microenvironment responsiveness present strong potential for clinical wound management applications.
Effective management of bacterial infection and inflammatory responses is critical for accelerating infected wound healing. Herein, we developed a multifunctional hydrogel microneedle system (GMPL@GC MNs) integrating gelatin methacryloyl (GM) as the matrix material, ε-poly-L-lysine (PL) for enhanced antibacterial properties, and gelatin-curcumin nanoparticles (GC NPs) for inflammation modulation. The system exploits elevated matrix metalloproteinase (MMP) levels at wound sites to trigger specific degradation of GC NPs and controlled curcumin (Cur) release. This MMP-responsive mechanism enables reactive oxygen species (ROS) scavenging, inflammatory response suppression, and sustained drug delivery over 96 h. In bacteria-infected wound models, the GMPL@GC MNs demonstrated excellent therapeutic efficacy, achieving 99.1% wound closure rate within 10 days through combined antibacterial, antioxidant, and anti-inflammatory actions. The system's multifunctionality and microenvironment responsiveness present strong potential for clinical wound management applications.
通讯机构：
[Guan, H ; Liu, XH] H;Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China.;Beijing Inst Radiat Med, Dept Radiat Biol, Beijing Key Lab Radiobiol, Beijing 100850, Peoples R China.
关键词：
DNA methylation;biomarkers;epigenetics;ionizing radiation;radiation exposure assessment;radiation protection
摘要：
DNA methylation is a common endogenous chemical modification in eukaryotic DNA, primarily involving the covalent attachment of a methyl group to the fifth carbon of cytosine residues, leading to the formation of 5-methylcytosine (5mC). This epigenetic modification plays a crucial role in gene expression regulation and genomic stability maintenance in eukaryotic systems. Ionizing radiation (IR) has been shown to induce changes in global DNA methylation patterns, which exhibit significant temporal stability. This stability makes DNA methylation profiles promising candidates for radiation-specific biomarkers. This review systematically examines the impact of IR on genome-wide DNA methylation landscapes and evaluates their potential as molecular indicators of radiation exposure. Advancing the knowledge of radiation-induced epigenetic modifications in radiobiology contributes to a deeper understanding of IR-driven epigenetic reprogramming and facilitates the development of novel molecular tools for the early detection and quantitative risk assessment of radiation exposure.
期刊：
International Journal of Biological Macromolecules,2025年312:144090 ISSN：0141-8130
通讯作者：
Ningchao Zheng<&wdkj&>Xiangbiao Yin
作者机构：
[Zhang, Haoyu; Pan, Xiangni; Wu, Qiang; Wu, Yehuizi; Zheng, Ningchao; Ning, Shunyan; Zeng, Deqian; Chen, Lifeng; Li, Wenlong; Wang, Ji; Jiang, Tianjiao] School of Nuclear Science and Technology, University of South China, 28 Changsheng West Road, Hengyang 421001, China;[Long, Xizhi] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[Watabe, Hiroshi] Division of Radiation Protection and Safety Control, Cyclotron and Radioisotope Center, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan;[Wu, Hao; Wu, Yan] School of Nuclear Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China;Key Laboratory of Advanced Nuclear Energy Design and Safety, Ministry of Education, University of South China, 28 Changsheng West Road, Hengyang, China
通讯机构：
[Ningchao Zheng; Xiangbiao Yin] S;School of Nuclear Science and Technology, University of South China, 28 Changsheng West Road, Hengyang 421001, China<&wdkj&>Key Laboratory of Advanced Nuclear Energy Design and Safety, Ministry of Education, University of South China, 28 Changsheng West Road, Hengyang, China<&wdkj&>School of Nuclear Science and Technology, University of South China, 28 Changsheng West Road, Hengyang 421001, China
摘要：
Transarterial radioembolization (TARE) using yttrium-90 ( 90 Y)-labeled glass and resin microspheres is an emerging therapeutic technique for hepatocellular carcinoma (HCC). However, the non-biodegradability and rapid settlement of current commercial microspheres might hinder their even distribution and repetitive administration thus causing unsatisfactory therapeutic effects. In this context, novel functional chitosan-based microspheres (CPIs) that can efficiently label Y as a favorable TARE material were developed for the first time by successive grafting poly (glycidyl methacrylate) (PGMA) and iminodiacetic acid (IDA) onto chitosan microspheres (CMs). The results confirmed that the CPIs had desirable spherical shapes with average diameters of around 20.9 μm, an ideal settlement rate within 5 min, and considerable biodegradability at 10th weeks. It reached Y adsorption equilibrium within 30 min and maintained the maximum adsorption capacity up to 14.95 mg g −1 at pH 6.0 following pseudo-second-order kinetic and Langmuir models. Additionally, Y-labeled CPIs were rather stable in vitro, for which Y would firmly interact with the sodium carboxylate group and tertiary amine nitrogen atoms on IDA, and its leakage when shaken in phosphate-buffered saline for 24 h was barely detected. Altogether, these properties of the as-developed CPIs hold great potential as promising radioembolization microspheres for TARE therapy against liver cancer.
Transarterial radioembolization (TARE) using yttrium-90 ( 90 Y)-labeled glass and resin microspheres is an emerging therapeutic technique for hepatocellular carcinoma (HCC). However, the non-biodegradability and rapid settlement of current commercial microspheres might hinder their even distribution and repetitive administration thus causing unsatisfactory therapeutic effects. In this context, novel functional chitosan-based microspheres (CPIs) that can efficiently label Y as a favorable TARE material were developed for the first time by successive grafting poly (glycidyl methacrylate) (PGMA) and iminodiacetic acid (IDA) onto chitosan microspheres (CMs). The results confirmed that the CPIs had desirable spherical shapes with average diameters of around 20.9 μm, an ideal settlement rate within 5 min, and considerable biodegradability at 10th weeks. It reached Y adsorption equilibrium within 30 min and maintained the maximum adsorption capacity up to 14.95 mg g −1 at pH 6.0 following pseudo-second-order kinetic and Langmuir models. Additionally, Y-labeled CPIs were rather stable in vitro, for which Y would firmly interact with the sodium carboxylate group and tertiary amine nitrogen atoms on IDA, and its leakage when shaken in phosphate-buffered saline for 24 h was barely detected. Altogether, these properties of the as-developed CPIs hold great potential as promising radioembolization microspheres for TARE therapy against liver cancer.
期刊：
Sensors and Actuators B-Chemical,2025年422:136598 ISSN：0925-4005
通讯作者：
Rulin Feng
作者机构：
School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;Department of Chemistry, Fudan University, Shanghai 200433, China;State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants, Hunan Ecology and Environment Monitoring Center, Changsha 410019, China;Fujian Key Laboratory of Functional Marine Sensing Materials, Minjiang University, Fuzhou 350108, China;[Diao, Qiaoqiao; Bu, Zhijian; Chen, Xinyu; Liu, Jinjin; Tang, Zheng; Liang, Hao; Tian, Qingzhen; Li, Shu] School of Public Health, Hengyang Medical School, University of South China,Hengyang 421001,China
通讯机构：
[Rulin Feng] D;Department of Chemistry, Fudan University, Shanghai 200433, China
摘要：
As a highly toxic heavy metal pollutant, mercury ions cause substantial risks to the environment and human health, and developing high-performance and convenient analytical approaches becomes quite important. Here we propose a performance-complementary colorimetric/electrochemical dual-mode Hg2+ sensing method based on the analyte accelerating the peroxidase-mimetic activity of gold nanoparticles (AuNPs) loaded on graphene oxide (GO). The GO-AuNPs composite was readily fabricated via mechanically mixing AuNPs and GO with controllable proportions, exhibiting a weak peroxidase-like activity in catalyzing the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Upon the introduction of Hg2+, it combined with AuNPs rapidly, significantly enhancing the peroxidase-mimicking activity of GO-AuNPs. By capturing the color signal of the generated blue oxTMB and the electro-oxidation response of residual TMB, a “turn-on” colorimetric and “turn-off” electrochemical bimodal method was established for Hg2+ measurement, providing a linear range of 10–60 μg/L in colorimetric detection and 0.001–20 μg/L in electrochemical analysis. Different from common bimodal assays mainly improving detecting reliability due to their cross-validation ability, our dual-mode sensor exhibits the unique feature of complementary sensitivity and detection range, thus enabling its flexible use in analyzing different levels of the target in a broad scope with no requirement for sample enrichment and dilution.
As a highly toxic heavy metal pollutant, mercury ions cause substantial risks to the environment and human health, and developing high-performance and convenient analytical approaches becomes quite important. Here we propose a performance-complementary colorimetric/electrochemical dual-mode Hg2+ sensing method based on the analyte accelerating the peroxidase-mimetic activity of gold nanoparticles (AuNPs) loaded on graphene oxide (GO). The GO-AuNPs composite was readily fabricated via mechanically mixing AuNPs and GO with controllable proportions, exhibiting a weak peroxidase-like activity in catalyzing the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Upon the introduction of Hg2+, it combined with AuNPs rapidly, significantly enhancing the peroxidase-mimicking activity of GO-AuNPs. By capturing the color signal of the generated blue oxTMB and the electro-oxidation response of residual TMB, a “turn-on” colorimetric and “turn-off” electrochemical bimodal method was established for Hg2+ measurement, providing a linear range of 10–60 μg/L in colorimetric detection and 0.001–20 μg/L in electrochemical analysis. Different from common bimodal assays mainly improving detecting reliability due to their cross-validation ability, our dual-mode sensor exhibits the unique feature of complementary sensitivity and detection range, thus enabling its flexible use in analyzing different levels of the target in a broad scope with no requirement for sample enrichment and dilution.
作者机构：
[Lei Zhang; Kaimin Chen; Luchang Li; Xiu Wei; Xianxin Cai; Yaohui Yu; Yixian Yang; Renping Cao; Fenghua Wei; Bang Lan; Xiaohu Luo; Hui Liang] Northeast Guangdong Key Laboratory of New Functional Materials, Guangdong Rare Earth Photofunctional Materials Engineering Technology Research Center, Meizhou Rare Earth Photo functional Materials Engineering Technology Research Center, Office of Assets&Laboratory Management, School of Chemistry and Environment, Jiaying University, Meizhou, 514015, China;[Deshuai Zhen] Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Yanhua Li] School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang, 421001, China
通讯机构：
[Yanhua Li] S;[Hui Liang] N;[Deshuai Zhen] H;Northeast Guangdong Key Laboratory of New Functional Materials, Guangdong Rare Earth Photofunctional Materials Engineering Technology Research Center, Meizhou Rare Earth Photo functional Materials Engineering Technology Research Center, Office of Assets&Laboratory Management, School of Chemistry and Environment, Jiaying University, Meizhou, 514015, China<&wdkj&>School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang, 421001, China<&wdkj&>Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China
摘要：
Developing non-precious electrocatalysts with high-efficiency hydrogen evolution reaction (HER) activity for hydrogen production at all pH values is crucial for the application and promotion of water electrolysis. Herein, a unique structure of nickel-cobalt (Ni-Co) nanoparticles@Ni 0.19 Co 0.26 P nanowires core/shell arrays on Ni foam as a pH-universal electrocatalyst for hydrogen evolution is synthesized by a simple hydrothermal process and thermal reduction treatment. The morphological analysis reveals that numerous Ni-Co nanoparticles are densely packed inside the hollow interior of Ni 0.19 Co 0.26 P nanowires. This unique structure integrates the advantages of high surface area from the nanowires and enhanced electronic conductivity from the Ni-Co nanoparticles, effectively addressing the conventional issue of slow charge transfer rate in 3D metal-based compounds, which is typically caused by a long transfer distance. The electrocatalysts provide an excellent hydrogen evolution reaction performance in alkaline, neutral, and acidic media with an overpotential of 35, 36, and 23 mV to reach the current density of 10 mA/cm 2 , respectively. This study highlights the potential of Ni-Co nanoparticles@Ni 0.19 Co 0.26 P nanowires as a cost-effective and pH-universal HER electrocatalyst, offering promising prospects for sustainable hydrogen production.
Developing non-precious electrocatalysts with high-efficiency hydrogen evolution reaction (HER) activity for hydrogen production at all pH values is crucial for the application and promotion of water electrolysis. Herein, a unique structure of nickel-cobalt (Ni-Co) nanoparticles@Ni 0.19 Co 0.26 P nanowires core/shell arrays on Ni foam as a pH-universal electrocatalyst for hydrogen evolution is synthesized by a simple hydrothermal process and thermal reduction treatment. The morphological analysis reveals that numerous Ni-Co nanoparticles are densely packed inside the hollow interior of Ni 0.19 Co 0.26 P nanowires. This unique structure integrates the advantages of high surface area from the nanowires and enhanced electronic conductivity from the Ni-Co nanoparticles, effectively addressing the conventional issue of slow charge transfer rate in 3D metal-based compounds, which is typically caused by a long transfer distance. The electrocatalysts provide an excellent hydrogen evolution reaction performance in alkaline, neutral, and acidic media with an overpotential of 35, 36, and 23 mV to reach the current density of 10 mA/cm 2 , respectively. This study highlights the potential of Ni-Co nanoparticles@Ni 0.19 Co 0.26 P nanowires as a cost-effective and pH-universal HER electrocatalyst, offering promising prospects for sustainable hydrogen production.
通讯机构：
[Yang, SY; Li, L; Zhen, DS ] U;Univ South China, Affiliated Hosp 1, Canc Res Inst, Sch Publ Hlth,Hengyang Med Sch, Hengyang 421001, Peoples R China.
关键词：
Covalent organic frameworks;Uranyl ion;One-step condensation reaction;Density functional theory
摘要：
Nuclear accidents, uranium mining, and nuclear weapons production pose significant health and environmental concerns, and as a consequence there is ongoing interest in techniques for rapid and selective detection of the uranyl ion (UO 2 2+ ). In this study, a β-keto-enamine-linked covalent organic framework (COF) fluorescent probe was synthesized by a simple one-step condensation reaction using 1,3,5-triformylphoroglucinol (Tp) and 3,3′-dihydroxybenzidine (Db). Tp-Db exhibited a UO 2 2+ detection limit of 99.34 nM and a 10 s reaction time, making it suitable for rapid detection in diverse environmental samples including river water, nuclear wastewater, food, and urine. Experimental analyses and density-functional theory (DFT) calculations reveal that UO 2 2+ preferentially coordinates with the carbonyl group in Tp-Db through intramolecular charge transfer and electrostatic interactions, and the presence of the hydroxyl assistant group further improves the binding affinity. This study elucidates the interaction mechanism of different functional groups (carbonyl, hydroxyl, and imine) with UO 2 2+ within the channels of the keto-enamine-linked COF, providing a promising rational basis for the development of an advanced UO 2 2+ sensing platform.
Nuclear accidents, uranium mining, and nuclear weapons production pose significant health and environmental concerns, and as a consequence there is ongoing interest in techniques for rapid and selective detection of the uranyl ion (UO 2 2+ ). In this study, a β-keto-enamine-linked covalent organic framework (COF) fluorescent probe was synthesized by a simple one-step condensation reaction using 1,3,5-triformylphoroglucinol (Tp) and 3,3′-dihydroxybenzidine (Db). Tp-Db exhibited a UO 2 2+ detection limit of 99.34 nM and a 10 s reaction time, making it suitable for rapid detection in diverse environmental samples including river water, nuclear wastewater, food, and urine. Experimental analyses and density-functional theory (DFT) calculations reveal that UO 2 2+ preferentially coordinates with the carbonyl group in Tp-Db through intramolecular charge transfer and electrostatic interactions, and the presence of the hydroxyl assistant group further improves the binding affinity. This study elucidates the interaction mechanism of different functional groups (carbonyl, hydroxyl, and imine) with UO 2 2+ within the channels of the keto-enamine-linked COF, providing a promising rational basis for the development of an advanced UO 2 2+ sensing platform.
摘要：
We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu(2+) ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu(2+). Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an "off-on-off" fluorescence response. The detection limits of this method for PPi and ALP were 103nmoldm(-3) and 0.18 U dm(-3), respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.
作者机构：
[Yang, Ying; Feng, Zhi; Zhang, Yu; Liao, Yi-jun; Jiang, Hui; Luo, Yu-hang; Chen, Jue-miao; Wei, Bo] Institute of Translational Medicine, School of Basic Medical, Department of Special Medicine, School of Public Health, Hengyang Medical College, University of South China, Hengyang, 421001, China;[Long, Yinxi] Department of Neurology, Affiliated Hengyang Hospital of Hunan Normal University &;[Long, Yinxi] amp Hengyang Central Hospital, Hengyang, 421001, China
摘要：
Fatigue, commonly experienced in daily life, is a feeling of extreme tiredness, shortage or lack of energy, exhaustion, and difficulty in performing voluntary tasks. Central fatigue, defined as a progressive failure to voluntarily activate the muscle, is typically linked to moderate- or light-intensity exercise. However, in some instances, high-intensity exercise can also trigger the onset of central fatigue. Exercise-induced central fatigue often precedes the decline in physical performance in well-trained athletes. This leads to a reduction in nerve impulses, decreased neuronal excitability, and an imbalance in brain homeostasis, all of which can adversely impact an athlete's performance and the longevity of their sports career. Therefore, implementing strategies to delay the onset of exercise-induced central fatigue is vital for enhancing athletic performance and safeguarding athletes from the debilitating effects of fatigue. In this review, we discuss the structural basis, measurement methods, and biomarkers of exercise-induced central fatigue. Furthermore, we propose non-pharmacological interventions to mitigate its effects, which can potentially foster improvements in athletes' performances in a healthful and sustainable manner.
作者机构：
[Li, Yuanxiu; Shi, Sicheng; Liu, Qijing; "Li, Feng; Yu, Huan; Wu, Shengbo; An, Xingjuan; Zhang, Baocai; Li, Chao; You, Zixuan; Tang, Rui] State Key Laboratory of Synthetic Biology, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China;[Yu, Huan; Zhang, Baocai] College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China;[Long, Xizi] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China;[Shi, Liang] Department of Biological Sciences and Technology, School of Environmental Studies, China University of Geoscience in Wuhan, Wuhan, Hubei, 430074, China;[Nealson, Kenneth H.] Departments of Earth Science & Biological Sciences, University of Southern California, 4953 Harriman Ave., South Pasadena, CA, 91030, USA
通讯机构：
[Hao Song] S;State Key Laboratory of Synthetic Biology, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, China<&wdkj&>College of Life and Health Sciences, Northeastern University, Shenyang, China
摘要：
Electron shuttle plays a decisive role in extracellular electron transfer (EET) of exoelectrogens. However, neither identifying the most efficient electron shuttle molecule nor programming its optimal synthesis level that boosts EET has been established. Here, the phenazine-1-carboxylic acid (PCA) biosynthesis pathway is first constructed to synthesize PCA at an optimal level for EET in Shewanella oneidensis MR-1. To facilitate PCA transport, the porin OprF is expressed to improve cell membrane permeability, the cytotoxicity of which, however, impaired cell growth. To mitigate cytotoxicity, PCA biosensor is designed to dynamically decouple PCA biosynthesis and transport, resulting in the maximum output power density reaching 2.85 ± 0.10 W m−2, 33.75-fold higher than wild-type strain. Moreover, extensive analyses of cellular electrophysiology, metabolism, and behaviors reveal PCA shuttles electrons from cell to electrode, which is the dominant mechanism underlying PCA-boosted EET. We conclude dynamic synthesis and transport of PCA is an efficient strategy for enhancing EET. Electron shuttle-mediated extracellular electron transfer (EET) is most critical in determining the efficiency of chemical-to-power of exoelectrogens. Here, the authors show that addition of phenazine-1-carboxylic acid at ~80 µM leads to the improved power generation in Shewenella oneidensis MR-1.
摘要：
Rapid and accurate detection of Chlamydia psittaci , the causative agent of psittacosis, is crucial for both human and animal health but presents significant challenges, particularly in grassroots health institutions. Our previous PDTCTR fluorescence sensing platform, which combined the engineered Cas12f1_ge4.1 system with recombinase polymerase amplification (RPA), significantly enhanced detection efficiency. However, its requirement for specialized equipment, costly RPA reagents, and absence of visual output restricted its practical application in such environments. To address these limitations, we developed the ERA/Cas12f1_ge4.1 system, integrating Cas12f1_ge4.1 with the cost-effective Enzymatic Recombinase Amplification (ERA). This system enables sensitive detection of Chlamydia psittaci double-stranded DNA within 50 min through both fluorescence and colloidal gold lateral flow assay strips. The platform achieves detection limits of 10 copies/μL for fluorescence and 100 copies/μL for lateral flow. Clinical validation involving 93 parrot samples demonstrated high performance in both detection modes. Fluorescence detection achieved 95.4 % sensitivity, 100 % specificity, a 100 % positive predictive value (PPV), and a 90.3 % negative predictive value (NPV). Meanwhile, the lateral flow assay exhibited 92.3 % sensitivity, 100 % specificity, 100 % PPV, and an 84.8 % NPV. The ERA/Cas12f1_ge4.1 system offers a rapid, accurate, cost-effective, and visually interpretable diagnostic tool suitable for both laboratory and community health centers. This advancement holds significant potential for improving psittacosis diagnosis and control, particularly in resource-limited environments.
Rapid and accurate detection of Chlamydia psittaci , the causative agent of psittacosis, is crucial for both human and animal health but presents significant challenges, particularly in grassroots health institutions. Our previous PDTCTR fluorescence sensing platform, which combined the engineered Cas12f1_ge4.1 system with recombinase polymerase amplification (RPA), significantly enhanced detection efficiency. However, its requirement for specialized equipment, costly RPA reagents, and absence of visual output restricted its practical application in such environments. To address these limitations, we developed the ERA/Cas12f1_ge4.1 system, integrating Cas12f1_ge4.1 with the cost-effective Enzymatic Recombinase Amplification (ERA). This system enables sensitive detection of Chlamydia psittaci double-stranded DNA within 50 min through both fluorescence and colloidal gold lateral flow assay strips. The platform achieves detection limits of 10 copies/μL for fluorescence and 100 copies/μL for lateral flow. Clinical validation involving 93 parrot samples demonstrated high performance in both detection modes. Fluorescence detection achieved 95.4 % sensitivity, 100 % specificity, a 100 % positive predictive value (PPV), and a 90.3 % negative predictive value (NPV). Meanwhile, the lateral flow assay exhibited 92.3 % sensitivity, 100 % specificity, 100 % PPV, and an 84.8 % NPV. The ERA/Cas12f1_ge4.1 system offers a rapid, accurate, cost-effective, and visually interpretable diagnostic tool suitable for both laboratory and community health centers. This advancement holds significant potential for improving psittacosis diagnosis and control, particularly in resource-limited environments.
摘要：
Radiation therapy is an important method to treat liver cancer, but because of the strong DNA repair ability of liver cancer cells, even after receiving high doses of radiation still can not get satisfactory results. Atorvastatin (ATO) is a lipophilic and tissue-selective inhibitor of HMG-CoA reductase whose anticancer effects have been validated in various cells, but its effect on the radiation sensitivity of hepatocellular carcinoma cells remains unclear. Therefore, Therefore, this study explored the radiosensitivity of ATO and its possible mechanism by pretreating HepG2 with ATO and collecting HepG2 cells after irradiation. It was found that atorvastatin can not only affect the survival of liver cancer cells when used alone, but also enhance the radiation sensitivity of HepG2 cells. The study found that ATO significantly exacerbated the inhibitory effects of IR on the growth, proliferation, and migration of HepG2 cells. Measurement of ROS, SOD, GPx, and MDA levels indicated that ATO enhanced IR-induced oxidative stress, further promoted the decrease of Mitochondrial Membrane Potential, increased the rate of apoptosis in HepG2, upregulating pro-apoptotic proteins Bax and Cleaved-Caspase 3, and downregulating anti-apoptotic proteins Bcl-2. Western blot analysis showed that the PI3K-Akt-mTOR pathway was inhibited, leading to the activation of cytotoxic autophagy in HepG2 and an increase in the expression of the LC-3II protein. In summary, ATO, in combination with IR, enhances the oxidative stress response of HepG2 induced by IR, promotes autophagy by inhibiting the PI3K-Akt-mTOR pathway, and thereby potentially enhances the radiosensitivity of HepG2 as a pharmacological intervention.
摘要：
Mercury is a highly toxic substance that can enter the human body through various pathways and accumulate, leading to chronic or acute poisoning. Accurate detection of mercury ions (Hg 2+ ) is crucial for preventing and controlling mercury pollution, as well as safeguarding human health and ecological safety. Herein, a simple and effective electrochemical sensing was designed for rapid determining mercury ion (Hg 2+ ) by 2D boat-sheet-like Co (II) metal–organic framework (Co-MOF). In this approach, 2D Co-MOF, characterized by its porous framework and high specific surface area, was integrated onto the surface of screen-printed carbon electrodes (SPCE) to enhance the electrode’s active surface area. Consequently, Hg 2+ had been enriched and measured using square wave anodic stripping voltammetry. Optimized electrochemical sensing parameters yielded calibration curves with excellent linearity over the standard range of 0.7 to 80 μmol/L. The detection limit was found to be as low as 15.09 nmol/L, which was significantly below the World Health Organization’s reference range for normal mercury levels in urine (50–100 nmol/L). The sensing exhibited remarkable selectivity, repeatability (RSD: 0.91 %), and stability (RSD: 2.97 %), without significant interference from other heavy metal ions. Furthermore, the method demonstrated promising practical applicability in the analysis of real urine samples, with recovery rates varying from 92.80 % to 107.99 %.
Mercury is a highly toxic substance that can enter the human body through various pathways and accumulate, leading to chronic or acute poisoning. Accurate detection of mercury ions (Hg 2+ ) is crucial for preventing and controlling mercury pollution, as well as safeguarding human health and ecological safety. Herein, a simple and effective electrochemical sensing was designed for rapid determining mercury ion (Hg 2+ ) by 2D boat-sheet-like Co (II) metal–organic framework (Co-MOF). In this approach, 2D Co-MOF, characterized by its porous framework and high specific surface area, was integrated onto the surface of screen-printed carbon electrodes (SPCE) to enhance the electrode’s active surface area. Consequently, Hg 2+ had been enriched and measured using square wave anodic stripping voltammetry. Optimized electrochemical sensing parameters yielded calibration curves with excellent linearity over the standard range of 0.7 to 80 μmol/L. The detection limit was found to be as low as 15.09 nmol/L, which was significantly below the World Health Organization’s reference range for normal mercury levels in urine (50–100 nmol/L). The sensing exhibited remarkable selectivity, repeatability (RSD: 0.91 %), and stability (RSD: 2.97 %), without significant interference from other heavy metal ions. Furthermore, the method demonstrated promising practical applicability in the analysis of real urine samples, with recovery rates varying from 92.80 % to 107.99 %.
