摘要：
Designing high-performance photocatalytic semiconductor catalysts remains a formidable challenge in energy conversion and environmental remediation. This study develops three-dimensionally ordered mesoporous carbon nitride photonic crystals (CNPC) with synergistic nitrogen vacancies and inverse opal structures via a hard-templating method using nitrogen-rich precursors. Urea-derived CNPCU achieves 96.95 % U(Ⅵ) removal after 60 min photoactivation followed by 3 h dark reaction, substantially outperforming unmodified UCN. Mechanistic studies reveal that photogenerated electrons reduce O 2 to ·O 2 - via a two-step single-electron pathway, which protonates to form H 2 O 2 . This species drives U(Ⅵ) reduction and subsequent precipitation as metastudtite ((UO 2 )O 2 ·2 H 2 O), identified by XRD. Nitrogen vacancies optimize charge separation and generate active sites at the atomic level, while the microscale inverse opal structure enhances light harvesting via the slow-photon effect. Their synergistic interaction underpins such exceptional photocatalytic activity. The material demonstrates > 90 % efficiency retention over 5 cycles and strong ion interference resistance, establishing a dual-scale engineering strategy for advanced metal-free photocatalysts.
Designing high-performance photocatalytic semiconductor catalysts remains a formidable challenge in energy conversion and environmental remediation. This study develops three-dimensionally ordered mesoporous carbon nitride photonic crystals (CNPC) with synergistic nitrogen vacancies and inverse opal structures via a hard-templating method using nitrogen-rich precursors. Urea-derived CNPCU achieves 96.95 % U(Ⅵ) removal after 60 min photoactivation followed by 3 h dark reaction, substantially outperforming unmodified UCN. Mechanistic studies reveal that photogenerated electrons reduce O 2 to ·O 2 - via a two-step single-electron pathway, which protonates to form H 2 O 2 . This species drives U(Ⅵ) reduction and subsequent precipitation as metastudtite ((UO 2 )O 2 ·2 H 2 O), identified by XRD. Nitrogen vacancies optimize charge separation and generate active sites at the atomic level, while the microscale inverse opal structure enhances light harvesting via the slow-photon effect. Their synergistic interaction underpins such exceptional photocatalytic activity. The material demonstrates > 90 % efficiency retention over 5 cycles and strong ion interference resistance, establishing a dual-scale engineering strategy for advanced metal-free photocatalysts.
关键词：
25-hydroxyvitamin D;Gene polymorphism;Osteocalcin;Procollagen type 1 N-terminal propeptide;β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide
摘要：
Objective This study aimed to investigate possible serum 25-hydroxyvitamin D [25(OH)D] cutoffs for the associations between 25(OH)D and Bone turnover markers (BTMs), and how GC gene variation influences such cutoffs in Chinese women of childbearing age.
This study aimed to investigate possible serum 25-hydroxyvitamin D [25(OH)D] cutoffs for the associations between 25(OH)D and Bone turnover markers (BTMs), and how GC gene variation influences such cutoffs in Chinese women of childbearing age.
Methods In total, 1,505 non-pregnant or non-lactating women (18–45 years) were recruited from the 2015 Chinese Adult Chronic Disease and Nutrition Surveillance. Serum 25(OH)D, osteocalcin (OC), procollagen type 1 N-terminal propeptide (P1NP), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), and single nucleotide polymorphisms were determined. Locally weighted regression and smoothing scatterplot and segmented regression were performed to estimate the 25(OH)D thresholds.
In total, 1,505 non-pregnant or non-lactating women (18–45 years) were recruited from the 2015 Chinese Adult Chronic Disease and Nutrition Surveillance. Serum 25(OH)D, osteocalcin (OC), procollagen type 1 N-terminal propeptide (P1NP), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), and single nucleotide polymorphisms were determined. Locally weighted regression and smoothing scatterplot and segmented regression were performed to estimate the 25(OH)D thresholds.
Results The median serum 25(OH)D was 16.63 (11.96–22.55) ng/mL and the prevalence of low serum 25(OH)D (< 12 ng/mL) was 25.2%. Women with the lowest 25(OH)D had the highest β-CTX. After adjustment for the confounders, 25(OH)D cutoffs for OC [14.04 (12.84–15.23) ng/mL], β-CTX [13.94 (12.49–15.39) ng/mL], and P1NP [13.87 (12.37–15.37) ng/mL] in the whole population, cutoffs for OC [12.30 (10.68–13.91) ng/mL], β-CTX [12.23 (10.22–14.23) ng/mL], and P1NP [11.85 (10.40–13.31) ng/mL] in women with the GC rs2282679 G allele, and cutoffs for OC [12.75 (11.81–13.68) ng/mL], β-CTX [13.05 (11.78–14.32) ng/mL], and P1NP [12.81 (11.57–14.06) ng/mL] in women with the GC rs2282679 T allele, were observed. Below these cutoffs, BTMs were negatively associated with 25(OH)D, while above these cutoffs, BTMs plateaued.
The median serum 25(OH)D was 16.63 (11.96–22.55) ng/mL and the prevalence of low serum 25(OH)D (< 12 ng/mL) was 25.2%. Women with the lowest 25(OH)D had the highest β-CTX. After adjustment for the confounders, 25(OH)D cutoffs for OC [14.04 (12.84–15.23) ng/mL], β-CTX [13.94 (12.49–15.39) ng/mL], and P1NP [13.87 (12.37–15.37) ng/mL] in the whole population, cutoffs for OC [12.30 (10.68–13.91) ng/mL], β-CTX [12.23 (10.22–14.23) ng/mL], and P1NP [11.85 (10.40–13.31) ng/mL] in women with the GC rs2282679 G allele, and cutoffs for OC [12.75 (11.81–13.68) ng/mL], β-CTX [13.05 (11.78–14.32) ng/mL], and P1NP [12.81 (11.57–14.06) ng/mL] in women with the GC rs2282679 T allele, were observed. Below these cutoffs, BTMs were negatively associated with 25(OH)D, while above these cutoffs, BTMs plateaued.
Conclusion In Chinese women of childbearing age, there were thresholds effect of serum 25(OH)D concentrations on BTMs. The results indicated that serum 25(OH)D concentrations < 13.87 ng/mL in this population had adverse influences on maintaining bone remodeling. BTMs were suppressed at a relatively lower serum 25(OH)D in women with the GC rs2282679 G allele compared with those with the T allele.
In Chinese women of childbearing age, there were thresholds effect of serum 25(OH)D concentrations on BTMs. The results indicated that serum 25(OH)D concentrations < 13.87 ng/mL in this population had adverse influences on maintaining bone remodeling. BTMs were suppressed at a relatively lower serum 25(OH)D in women with the GC rs2282679 G allele compared with those with the T allele.
期刊：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES,2025年88(8):339-348 ISSN：1528-7394
通讯作者：
Liu, Jun;Zhan, CH
作者机构：
[Liu, Jun; Zhan, Chunhua; Yang, Yue; Yang, Fei; Wang, Yaqi] Univ South China, Sch Basic Med Sci, Sch Publ Hlth, Hunan Key Lab Typ Environm Pollut & Hlth Hazards,H, Hengyang 421001, Hunan, Peoples R China.;[Zeng, Wen; Zhan, Chunhua] Cent Hosp Shaoyang, Dept Publ Hlth, Shaoyang, Peoples R China.;[Yuan, Mei] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Neurol, Hengyang, Hunan, Peoples R China.;[Zhan, Chunhua] Univ South China, Key Lab Rare Pediat Dis, Minist Educ, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liu, J; Zhan, CH ] U;Univ South China, Sch Basic Med Sci, Sch Publ Hlth, Hunan Key Lab Typ Environm Pollut & Hlth Hazards,H, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Key Lab Rare Pediat Dis, Minist Educ, Hengyang 421001, Hunan, Peoples R China.
关键词：
MC-LR;Apoptosis;TNF-R1;RIPK1;HEK293
摘要：
In recent years, the outbreak of cyanobacterial blooms has become increasingly frequent. Microcystin-LR (MC-LR), a metabolite of cyanobacteria, poses a significant threat to the ecosystem and human health. Several studies have demonstrated that MC-LR might induce renal cell apoptosis, as a consequence of tissue damage. However, the molecular mechanisms underlying MC-LR-initiated renal injury remain to be determined. This investigation aimed to determine the role of apoptosis in MC-LR-induced kidney damage and its potential underlying mechanisms using the human embryonic kidney (HEK293) cell line. The results of TUNEL and immunofluorescence assays indicated that MC-LR induced increased apoptosis in HEK293 cells. Compared to control, the mRNA expression levels of RIPK1, caspase-8, and TNF-α were elevated following incubation with MC-LR, while the mRNA expression level of Bcl-2/Bax was decreased. The protein levels of RIPK1, TNF-R1, and caspase-8 were elevated in the MC-LR-treated HEK293 cells. Data demonstrated that MC-LR induced renal cell apoptosis through activation of the TNF-R1/RIPK1 pathway, providing new insights into understanding the toxic mechanisms attributed to MC-LR.
作者机构：
[Huang, Guanqin; Liu, Jianjun; Zhou, Li; Yang, Xifei; Wu, Desheng; Huang, Xinfeng; Gao, Chuanyue; Li, Shangming; Nie, Lulin; He, Kaiwu; Zhang, Huan; Yang, XF; Chen, Chongyang] Shenzhen Ctr Dis Control & Prevent, Shenzhen Key Lab Modern Toxicol, Shenzhen Med Key Discipline Hlth Toxicol 2020 2024, Shenzhen 518055, Peoples R China.;[Gao, Chuanyue] Xian Int Med Ctr Hosp, Xian 710100, Peoples R China.;[Yang, Deguang] Jinan Univ, Affiliated Hosp 5, Heyuan Shenhe Peoples Hosp, Dept Cardiol, Heyuan 517000, Peoples R China.;[Nie, Lulin] Jinan Univ, Coll Pharm, Guangzhou 510632, Peoples R China.;[Huang, Zhenlie] Southern Med Univ, Sch Publ Hlth, NMPA Key Lab Safety Evaluat Cosmet, Guangdong Prov Key Lab Trop Dis Res,Dept Toxicol, Guangzhou 510515, Peoples R China.
通讯机构：
[Yang, XF ; Zou, LY ] S;[Zhang, ZH ] U;Shenzhen Ctr Dis Control & Prevent, Shenzhen Key Lab Modern Toxicol, Shenzhen Med Key Discipline Hlth Toxicol 2020 2024, Shenzhen 518055, Peoples R China.;Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp,Clin Med Coll 2,Dept Neurol, Shenzhen, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
关键词：
Amyotrophic lateral sclerosis;Copper;Mitophagy;Motor dysfunction;Urolithin A
摘要：
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease pathologically characterized by selective degeneration of motor neurons resulting in a catastrophic loss of motor function. The present study aimed to investigate the effect of copper (Cu) exposure on progression of ALS and explore the therapeutic effect and mechanism of Urolithin A (UA) on ALS. 0.13 PPM copper chloride drinking water was administrated in SOD1(G93A) transgenic mice at 6weeks, UA at a dosage of 50mg/kg/day was given for 6weeks after a 7-week Cu exposure. Motor ability was assessed before terminal anesthesia. Muscle atrophy and fibrosis, motor neurons, astrocytes and microglia in the spinal cord were evaluated by H&E, Masson, Sirius Red, Nissl and Immunohistochemistry Staining. Proteomics analysis, Western blotting and ELISA were conducted to detect protein expression. Mitochondrial adenosine triphosphate (ATP) and malondialdehyde (MDA) levels were measured using an assay kit. Cu-exposure worsened motor function, promoted muscle fibrosis, loss of motor neurons, and astrocyte and microglial activation. It also induced abnormal changes in mitochondria-related biological processes, leading to a significant reduction in ATP levels and an increase in MDA levels. Upregulation of P62 and downregulation of Parkin, PINK1, and LAMP1 were revealed in SOD1(G93A) mice with Cu exposure. Administration of UA activated mitophagy, modulated mitochondria dysfunction, reduced neuroinflammation, and improved gastrocnemius muscle atrophy and motor dysfunction in SOD1(G93A) mice with Cu exposure. Mitophagy plays critical role in ALS exacerbated by Cu exposure. UA administration may be a promising treatment strategy for ALS.
摘要：
Anti-aging of human skin has long been a significant concern for beauty enthusiasts, and the introduction of various ascorbic acid derivatives in cosmetics plays a crucial role in combating free radicals and maintaining healthy and radiant skin, thus their discernment and quantification act as pivotal benchmarks for appraising the quality of cosmetics. Herein, we proposed a bienzyme-mimicking cascade-stimulated aminoantipyrine oxidative self-coupling strategy to enable the time-resolved discrimination and quantification of various ascorbic acid derivatives in complex cosmetic matrices. A series of two-dimensional Ni-Mn bimetallic nanozymes (Ni 3-x Mn x O 4 ) were successfully synthesized via hydrothermal crystallization combined with doping engineering. Ni 3-x Mn x O 4 demonstrates outstanding hydrolase-like and oxidase-like properties within a single catalytic system. In-situ LC-MS analysis has verified the existence of dimer chromogenic intermediates resulting from the self-coupling of 4-AP via Schiff-base-like chemistry . The hydrolyzed products modulate the time-resolved output of the cascade signal, serving as recognition codes for the analytes. An on-site chemical analytical method has also been established and shows a good linear relationship within various phosphatidic/glucoside derivatives in a wide range of 0.01–105 μM, with a low LOD of 3–5 nM, approximately 0.001–0.5 times that of other reported methods. This study underscores the substantial potential of our novel sensor for on-site analysis of the actual antioxidant capacity of cosmetics, and it provides a controlled approach for the design of hydrolase/oxidase mimics, offering valuable insights for future enzyme engineering .
Anti-aging of human skin has long been a significant concern for beauty enthusiasts, and the introduction of various ascorbic acid derivatives in cosmetics plays a crucial role in combating free radicals and maintaining healthy and radiant skin, thus their discernment and quantification act as pivotal benchmarks for appraising the quality of cosmetics. Herein, we proposed a bienzyme-mimicking cascade-stimulated aminoantipyrine oxidative self-coupling strategy to enable the time-resolved discrimination and quantification of various ascorbic acid derivatives in complex cosmetic matrices. A series of two-dimensional Ni-Mn bimetallic nanozymes (Ni 3-x Mn x O 4 ) were successfully synthesized via hydrothermal crystallization combined with doping engineering. Ni 3-x Mn x O 4 demonstrates outstanding hydrolase-like and oxidase-like properties within a single catalytic system. In-situ LC-MS analysis has verified the existence of dimer chromogenic intermediates resulting from the self-coupling of 4-AP via Schiff-base-like chemistry . The hydrolyzed products modulate the time-resolved output of the cascade signal, serving as recognition codes for the analytes. An on-site chemical analytical method has also been established and shows a good linear relationship within various phosphatidic/glucoside derivatives in a wide range of 0.01–105 μM, with a low LOD of 3–5 nM, approximately 0.001–0.5 times that of other reported methods. This study underscores the substantial potential of our novel sensor for on-site analysis of the actual antioxidant capacity of cosmetics, and it provides a controlled approach for the design of hydrolase/oxidase mimics, offering valuable insights for future enzyme engineering .
期刊：
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,2025年51(1):e16120- ISSN：1341-8076
通讯作者：
Wu, CQ
作者机构：
[Wu, CQ; Xie, Yinghao; Wu, Chengqiu; Zhu, Qiong] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Liu, Jun] Hengyang Cent Dis Control & Prevent, Hengyang, Peoples R China.
通讯机构：
[Wu, CQ ] U;Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.
关键词：
CD1a;ovarian cancer;prognosis;recurrence;recurrent ovarian cancer
摘要：
OBJECTIVE: Explored the correlation between CD1a expression in recurrence and prognosis of ovarian cancer (OV). METHODS: The CD1a expression profile in OV, recurrent OV, and normal tissues, as well as corresponding clinical data, were obtained from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC), Gene Expression Omnibus (GEO), and Genotype Tissue Expression (GTEx) databases. Meanwhile, immunohistochemical detection of CD1a expression in normal and OV tissues. Kaplan-Meier curves were plotted to estimate the hazard ratio (HR) of survival in OV. In addition, the correlation between CD1a and immune cells in OV, as well as the CD1a expression profile and corresponding survival time in pan-cancer were obtained from TCGA database. RESULTS: CD1a was overexpressed in OV and was significantly under-expressed in recurrent OV (TCGA-OV, p < 0.0001 and ICGC-OV, p < 0.0001). CD1a immunohistochemistry is significantly overexpressed in OV compared to normal tissue (p < 0.05). Recurrent OV (ICGC, p < 0.001; GSE17260, p < 0.001; GSE32062, p < 0.05). The prognosis in OV was significantly better when CD1a is overexpressed compared to under-expressed (HR [low], 1.426: 95% confidence interval [CI], 0.912-2.128; p = 0.050). Meanwhile, the overexpression of CD1a has a better prognosis than low expression in OV and recurrent OV (p = 0.004, HR [low] = 2.462, 95%CI [1.346-4.504] and p = 0.011, HR [low] = 2.199, 95%CI [1.202-4.024]). In addition, CD1a expression was closely correlated with immune cells, the CD8+ T cells, macrophages, and NK cells, while uncharacterized cells were significantly different (p = 2.65e-6, p = 7.52e-13, p = 8.28e-12, and p = 5.89e-8, respectively). Moreover, CD1a expression affected the prognosis in various other cancers. CONCLUSIONS: CD1a expression affected the recurrence and prognosis of OV and is closely related to various immune cell levels.
作者机构：
[Zhao, Kunyan; Song, Fengmei; Yang, Fei; Tang, Yan] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Wu, Mingyang] Cent South Univ, Xiangya Sch Publ Hlth, Dept Maternal & Child Hlth, Changsha 410007, Peoples R China.;[Pan, Xiongfeng; Liu, Caixia; Qiu, Jun; Xiang, Shiting] Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Pediat Res Inst Hunan Prov,Xiangya Sch Med, Changsha 410007, Peoples R China.;[Peng, Yunlong] Soochow Univ, Dept Epidemiol & Hlth Stat, Med Coll, Suzhou 215123, Peoples R China.;[Cao, Yunhui; Wu, Sha] Univ South China, Hengyang Med Sch, Dept Pediat, Hengyang 421001, Peoples R China.
通讯机构：
[Qiu, J ] C;Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Pediat Res Inst Hunan Prov,Xiangya Sch Med, Changsha 410007, Peoples R China.
关键词：
Early life;Gut microbiota;Neonate;Thallium
摘要：
Previous research has found a correlation between heavy metals and gut microbiota in humans. However, there are few population-based studies examining the impact of early life thallium (Tl) exposure on neonatal microbiome . 342 newborns were recruited from Hunan Children's Hospital and subsequently divided into three groups (low, medium, and high) based on the 25th and 75th percentiles of serum Tl concentration. Additionally, the relationship between Tl and gut microbiota was analyzed in subgroups (preterm or full-term neonates). The association between Tl and gut microbiota in neonates was analyzed by Redundancy analysis, Spearman correlation analysis and MaAsLin2. The detection rate of Tl in neonates was 100%, with the median concentration of 0.021 μg/L. In all neonates, we found significant differences in the Chao1 and ACE indices of α-diversity in gut microbiota, and the relative abundances of Bacteroidota and Bacteroidetes were significantly different among groups ( p < 0.05). Following the covariate adjustment, Tl was negatively correlated with Gemmatimonadota (Coef = 0.265, p < 0.05) in preterm neonates. In full-term neonates, Tl exhibited a positive correlation with the relative abundance of Robinsoniella (Coef = 0.563, p = 0.009) and a negative correlation with that of Pseudomonas (Coef = - 0.592, p = 0.012). Tryptophan and renin-angiotensin system pathways might exert important roles in Tl exposure. This study indicated that Tl exposure was associated with changes in α-diversity and the composition of gut microbiota in neonates, with Gemmatimonadota being predominantly affected in preterm neonates and Robinsoniella and Pseudomonas in full-term neonates.
Previous research has found a correlation between heavy metals and gut microbiota in humans. However, there are few population-based studies examining the impact of early life thallium (Tl) exposure on neonatal microbiome . 342 newborns were recruited from Hunan Children's Hospital and subsequently divided into three groups (low, medium, and high) based on the 25th and 75th percentiles of serum Tl concentration. Additionally, the relationship between Tl and gut microbiota was analyzed in subgroups (preterm or full-term neonates). The association between Tl and gut microbiota in neonates was analyzed by Redundancy analysis, Spearman correlation analysis and MaAsLin2. The detection rate of Tl in neonates was 100%, with the median concentration of 0.021 μg/L. In all neonates, we found significant differences in the Chao1 and ACE indices of α-diversity in gut microbiota, and the relative abundances of Bacteroidota and Bacteroidetes were significantly different among groups ( p < 0.05). Following the covariate adjustment, Tl was negatively correlated with Gemmatimonadota (Coef = 0.265, p < 0.05) in preterm neonates. In full-term neonates, Tl exhibited a positive correlation with the relative abundance of Robinsoniella (Coef = 0.563, p = 0.009) and a negative correlation with that of Pseudomonas (Coef = - 0.592, p = 0.012). Tryptophan and renin-angiotensin system pathways might exert important roles in Tl exposure. This study indicated that Tl exposure was associated with changes in α-diversity and the composition of gut microbiota in neonates, with Gemmatimonadota being predominantly affected in preterm neonates and Robinsoniella and Pseudomonas in full-term neonates.
期刊：
Food Safety and Health,2025年3(1):89-99 ISSN：2835-1096
通讯作者：
Xiangheng Niu
作者机构：
[Ziyu Zhang; Lina Tang; Wenjie Sheng; Youyi Yang; Guolin Lai; Jinjin Liu] School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, China;[Xiangheng Niu] School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, China
通讯机构：
[Xiangheng Niu] S;School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, China
摘要：
AbstractAs a common food additive, excessive nitrite poses a great threat to human health, and monitoring its content in food products is of importance for healthy diet. Currently, the detection of nitrite content in food is primarily focused on fresh dishes, and there is a lack of research on monitoring the variations of nitrite in different foods under various storage conditions. In this study, we cascaded nanozyme catalysis with diazotization reaction and developed a ratiometric colorimetric assay to dynamically analyze nitrite in leftovers. First, nanoscale MnFe2O4 was synthesized as an oxidase mimic to catalyze colorless 3,3′,5,5′‐tetramethylbenzidine (TMB) oxidation to blue TMBox. Then, a diazotization process of the produced TMBox took place under the stimulation of nitrite, lowering the ultraviolet‐visible absorption signal (652 nm) assigned to TMBox and simultaneously generating a new signal at 445 nm ascribed to diazotized TMBox. Thus, a ratiometric colorimetric method could be constructed based on the above reversed variations of the two signals for high‐selectivity nitrite determination, providing a linear range of 1.76–180 μM and a detection limit of 0.12 μM. By employing the established assay to dynamically monitor nitrite in food products, it was found that the nitrite content in overnight leftovers was higher than that in fresh dishes, presenting an increasing trend with storage time. In addition, potential impacts of storage factors on the dynamics of nitrite content were investigated, providing some implications for food preservation and daily consumption.
作者机构：
[Dingxin Long] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[Fei Chen; Jing Cao] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China;[Bei Yu] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
通讯机构：
[Fei Chen] N;National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
关键词：
Carbon-14;Committed dose;14 C urea breath test;Occupational health
摘要：
Objective To monitor the radioactivity concentration of 14 C in the air of rooms used to perform 14 C urea breath tests, and evaluate the contamination status of 14 C released during such tests, and assess the occupational health risk to medical staff working in such areas.
To monitor the radioactivity concentration of 14 C in the air of rooms used to perform 14 C urea breath tests, and evaluate the contamination status of 14 C released during such tests, and assess the occupational health risk to medical staff working in such areas.
Methods 14 CO 2 in air was absorbed from the air and turned into calcium carbonate through a chemical reaction. Then, calcium carbonate was prepared into a suspension in a low-potassium glass vial. The sample was analyzed using a low-background liquid scintillation counter.
14 CO 2 in air was absorbed from the air and turned into calcium carbonate through a chemical reaction. Then, calcium carbonate was prepared into a suspension in a low-potassium glass vial. The sample was analyzed using a low-background liquid scintillation counter.
Results The radioactivity concentrations of 14 C in air of the breath-test rooms in hospitals and physical examination institutions range from 1.35 to 18.41 Bq/m 3 . The annual committed dose for medical staff was estimated to be between 2.01 × 10 −2 and 2.74 × 10 −1 μSv.
The radioactivity concentrations of 14 C in air of the breath-test rooms in hospitals and physical examination institutions range from 1.35 to 18.41 Bq/m 3 . The annual committed dose for medical staff was estimated to be between 2.01 × 10 −2 and 2.74 × 10 −1 μSv.
Conclusions Our results reveal a significant increase of 14 C radioactivity concentration in the air of breath-test rooms, but the resulting committed dose is much lower than the limits prescribed by the International Commission on Radiological Protection (ICRP). However, the potential risk of long-term exposure to low-dose radiation should be paid more attention.
Our results reveal a significant increase of 14 C radioactivity concentration in the air of breath-test rooms, but the resulting committed dose is much lower than the limits prescribed by the International Commission on Radiological Protection (ICRP). However, the potential risk of long-term exposure to low-dose radiation should be paid more attention.
关键词：
birth defects;cause of death;confidence intervals;hazard ratio;mortality rate under 5 years;person-years
摘要：
OBJECTIVE: To calculate the impact of birth defects (BDs) diagnosed during the perinatal period on the mortality of children under 5 years of age. METHODS: This was a retrospective cohort analysis. From the monitoring system, we collected all hospital delivery, BD monitoring, and death information for children under 5 years in Hunan Province from 2017-2022. These data were linked by ID number. Mortality rates and Cox proportional hazards models were used to compute hazard ratios (HRs) and 95% confidence intervals (CIs) for the impact of BDs on mortality in children under 5 years of age. RESULTS: Among 3,807,340 live-born children, 29,879 (0.8%) had at least one type of BDs during the perinatal period, with a total of 12,215,033 person-years of follow-up. The mortality rate of the BDs group was 14.5% (95% CI: 13.7-15.3) per 1,000 person-years, which was 11.6 times (HR = 11.6, 95% CI: 10.5-12.8) greater than that of the nondefect group. The mortality rate per 1,000 person-years of girls with BDs was higher than that of boys (15.4% vs. 13.5%). For the BDs group, congenital anomalies (CAs) were the most common cause of death (57.2%). Compared with children without BDs, those with BDs had elevated mortality risks for CAs (HR, 58.1; 95% CI, 42.7-79.0), digestive (HR = 16.5, 95% CI: 6.1-45.0) and respiratory system malformations (HR, 11.9; 95% CI, 7.9-17.8), and cancer (HR = 11.1 95% CI: 4.7-26.2). CONCLUSIONS: This study revealed that BDs were strongly associated with mortality under 5 years of age, especially in the first 28 days, for muscular, chromosomal, genetic, and nervous system abnormalities.
摘要：
Beryllium (Be) is a recognised environmental toxicant associated with pulmonary fibrosis. Epithelial-mesenchymal transition (EMT), a critical process in cell phenotype conversion, plays a key role in its pathophysiology. Methyltransferase-like 3 (METTL3), a major N6-methyladenosine methyltransferase, regulates gene expression and cellular functions. However, its role in Be-induced EMT remains unclear. In this study, human bronchial epithelial cell line (16HBE cells) were exposed to varying concentrations of beryllium sulphate (BeSO(4)) to assess changes in METTL3 expression. METTL3 overexpression vectors were constructed, and quantitative reverse transcription-polymerase chain reaction, western blotting and immunofluorescence were used to detect METTL3, EMT markers and Wingless/Integrated (Wnt)/β-catenin pathway proteins. The Wnt/β-catenin pathway inhibitor ICG-001 was also employed to explore the role of the Wnt/β-catenin pathway in BeSO(4)-induced EMT. The study demonstrated that BeSO(4) suppressed METTL3 expression, induced EMT and activated the Wnt/β-catenin pathway in 16HBE cells. Both METTL3 overexpression and ICG-001 pretreatment mitigated BeSO(4)-induced EMT and Wnt/β-catenin pathway activation. These findings suggest that METTL3 inhibits BeSO(4)-induced EMT by suppressing the Wnt/β-catenin pathway, offering novel mechanistic insights into beryllium toxicity and a potential therapeutic target for Be-related pulmonary fibrosis.
作者机构：
[Li, Gang; Han, Yang; Bai, Chenjun; Zhao, Hongling; Guan, Hua; Gao, Shanshan; Jia, Jin; Luo, Jinhua; Zhou, Ping-Kun; Liu, Xiaochang; Xie, Dafei; Guo, Hejiang; Xuan, Lihui; Gu, Yongqing; Tan, Jinpeng; Huang, Xin; Hu, Weixiang; Guan, H; Liu, Yuhao] Beijing Inst Radiat Med, Dept Radiat Biol, Beijing Key Lab Radiobiol, Beijing, Peoples R China.;[Huang, Ruixue; Luo, Jinhua; Xuan, Lihui] Cent South Univ, Xiangya Sch Publ Hlth, Dept Occupat & Environm Hlth, Changsha, Hunan, Peoples R China.;[Li, Zhongjun; Ran, Qian] Army Mil Med Univ, Affiliated Hosp 2, Lab Med Ctr, Dept Blood Transfus,Lab Radiat Biol, Chongqing, Peoples R China.;[Li, Gang; Jia, Jin; Zhou, Ping-Kun; Tan, Jinpeng] Univ South China, Hengyang Med Coll, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.;[Ma, Teng] Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Canc Res Ctr, Beijing, Peoples R China.
通讯机构：
[Guan, H; Zhou, PK ] B;[Huang, RX ] C;Beijing Inst Radiat Med, Dept Radiat Biol, Beijing Key Lab Radiobiol, Beijing, Peoples R China.;Cent South Univ, Xiangya Sch Publ Hlth, Dept Occupat & Environm Hlth, Changsha, Hunan, Peoples R China.;Univ South China, Hengyang Med Coll, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.
摘要：
Chemo-/radioresistance of malignant tumors hampers cancer control and increases patient mortality. Efficient repair of damaged DNA is critical for the maintenance of genomic integrity and fidelity of genetic information. In reverse, increased DNA repair capability in cancer cells contributes to chemo-/radioresistance of malignant tumors. DNA double-strand break (DSB) is the most serious DNA damage and is also the principal molecular basis of radiotherapy. Upon DNA damage, the Ku80 is recruited and forms a critical DNA-PK complex at the DSB sites with Ku70 and the catalytic subunit (DNA-PKcs) to initiate DNA repair. How DNA-PK is assembled and activated is not fully understood. Based on the identification of radiation-reduced Ku80 K568 crotonylation through quantitative global lysine crotonylome analysis, we reveal that Ku80 K568 is crotonylated by p300-CBP-associated factor (PCAF). Upon DNA damage, the K568cr is decrotonylated by HDAC8 (Histone deacetylase 8). Decrotonylation of K568cr empties this site for the subsequent SUMOylation of Ku80 by CBX4. The conversion of Ku80 from K568 crotonylation to SUMOylation facilitates the assembly of DNA-PK complex and autophosphorylation of DNA-PKcs S2056, consequently activating the DSB repair. Moreover, mutation disrupting the post-translational modification (PTM) of Ku80 K568 site sensitizes cancer cells to radiotherapy in tumor-bearing nude mice models. This study elucidates the conversion model between two different forms of PTMs in the regulation of DNA-PK complex assembly and DSB repair, highlighting this model's potential in controlling chemo-/radioresistance of malignant tumors, as well as expands the atlas of therapeutic targets.
期刊：
WATER AIR AND SOIL POLLUTION,2025年236(1):1-12 ISSN：0049-6979
通讯作者：
Xue, JH
作者机构：
[Wang, Xinqing; Huang, Boshi; Xue, Jinhua; Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Lin, Dongying] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jingjing; Ren, Lanxing] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Hunan, Peoples R China.
通讯机构：
[Xue, JH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.
关键词：
Cadmium (II);FR;PAN;Fluorescence quenching
摘要：
The objective of this study was to establish a novel method of fluorescence for the determination of cadmium using fluorescein (FR) and 1-(2-Pyridinylazo)-2-Naohthalenol (PAN). In the pH 9.75 buffer solution of the ammonia-ammonium chloride, the energy transfer between FR and the PAN can occur, which can result in the quenching of the fluorescence. The addition of Cd (II) to this system enhances the degree of quenching. This phenomenon has led to the establishment of a novel method for the determination of cadmium, which has been optimized in terms of the required experimental conditions. In the optimal experimental conditions, the fluorescence intensity of the system decreased linearly in the range of 7.81 x 10-7 to 2.14 x 10-5 mol L-1. The linear regression equation used in this study was F = 123.63 + 170.65c (x 10-6 mol L-1) under the correlation coefficient of 0.9951, the detection limit of 2.34 x 10-7 mol L-1, and the relative standard deviation of 0.18%. The proposed method was simple, sensitive, fast, and economical. The method can be employed for the determination of cadmium in real samples (tap-water, pond water and Xiangjiang River) with the results demonstrating consistency with the graphite furnace atomic absorption method (t-test). This approach offers an innovative method for the quantification of Cd (II).
摘要：
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of anthropogenic, persistent, and highly toxic PAH contaminants associated with developmental toxicity, 9-fluorenone (9-FLO) is a typical member of the OPAH family. Due to its ketone group, it has higher polarity, which results in increased solubility in water and greater potential for transport via atmospheric particles or water bodies. Polyethylene (PE), an amorphous polymer, is characterized by high diffusivity, high permeability, and a large internal molecular free volume, which confers a strong absorption capacity for organic pollutants. The effects of individual and combined exposures to these two common environmental pollutants on aquatic life remain unclear. In this study, we evaluated the effects of PE and 9-FLO exposure on growth, development, metabolism, and behavior using zebrafish as a model organism. We employed methods and techniques such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking, automated behavior analysis, microscopy imaging, and real-time fluorescence quantification. Zebrafish embryos at 2 h post-fertilization (hpf) were exposed to PE and 9-FLO, both individually and in combination. Our studies showed that exposure to PE or 9-FLO alone increases embryonic mortality and decreases hatchability compared to the control group. The 9-FLO group exhibited delayed hatching and inhibited larval length growth. The exposed groups showed a loose arrangement of telencephalic neurons, partial apoptosis, decreased dopamine (DA) content, increased serotonin (5-HT) content, decreased exercise capacity, reduced rhythmic amplitude, and increased rest time. The combined exposure group showed a slight alleviation of these effects compared to the single exposure groups but still exhibited significant differences from the control group. In summary, early exposure to PE and 9-FLO in zebrafish embryos, whether alone or in combination, affects growth, development, apoptosis, neurotransmitter release, and motor behavior of zebrafish neurons.
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of anthropogenic, persistent, and highly toxic PAH contaminants associated with developmental toxicity, 9-fluorenone (9-FLO) is a typical member of the OPAH family. Due to its ketone group, it has higher polarity, which results in increased solubility in water and greater potential for transport via atmospheric particles or water bodies. Polyethylene (PE), an amorphous polymer, is characterized by high diffusivity, high permeability, and a large internal molecular free volume, which confers a strong absorption capacity for organic pollutants. The effects of individual and combined exposures to these two common environmental pollutants on aquatic life remain unclear. In this study, we evaluated the effects of PE and 9-FLO exposure on growth, development, metabolism, and behavior using zebrafish as a model organism. We employed methods and techniques such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking, automated behavior analysis, microscopy imaging, and real-time fluorescence quantification. Zebrafish embryos at 2 h post-fertilization (hpf) were exposed to PE and 9-FLO, both individually and in combination. Our studies showed that exposure to PE or 9-FLO alone increases embryonic mortality and decreases hatchability compared to the control group. The 9-FLO group exhibited delayed hatching and inhibited larval length growth. The exposed groups showed a loose arrangement of telencephalic neurons, partial apoptosis, decreased dopamine (DA) content, increased serotonin (5-HT) content, decreased exercise capacity, reduced rhythmic amplitude, and increased rest time. The combined exposure group showed a slight alleviation of these effects compared to the single exposure groups but still exhibited significant differences from the control group. In summary, early exposure to PE and 9-FLO in zebrafish embryos, whether alone or in combination, affects growth, development, apoptosis, neurotransmitter release, and motor behavior of zebrafish neurons.
作者机构：
[Xia, Yuehui; Yuan, Jianhua] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Xia, Yuehui] Shanghai Construct Environm Technol Co Ltd, 700 Dongda Ming Rd, Shanghai 200080, Peoples R China.;[Wei, Xiaofei; Xia, Yuehui] Shanghai Shenhuan Environm Engn Co Ltd, 700 Dongda Ming Rd, Shanghai 200080, Peoples R China.
通讯机构：
[Yuan, JH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词：
Fluidic fill material;Mixing station sediment;Recycled fine aggregate;Resource utilization;mud
摘要：
The synergistic resource utilization of solid waste represents a critical scientific challenge in the field of environmental engineering. This study innovatively proposes a solid waste-based fluid-filled material (FFM) to address the significant physicochemical differences among multi-source solid wastes, including drinking water treatment residues (DWTR), mixing station sediments (MSS), recycled fine aggregates (RFA), and mud. The results demonstrate that by precisely controlling the fluidity (160–200 mm), water-to-solid ratio (0.52–0.87), and wet density (1.47–1.68 g/cm 3 ) of the material, FFM with excellent mechanical properties can be obtained, exhibiting 7-day and 28-day compressive strengths of 0.20–1.20 MPa and 0.40–1.40 MPa, respectively. Under the premise of meeting performance requirements, the mechanical properties of FFM significantly improve with increasing proportions of low-carbon composite cementitious material (LCCCM) and RFA, as well as with extended curing age. Through systematic optimization, the optimal mix ratio for FFM was determined as Lime∶ DWTR∶ MSS∶ RFA∶ mud∶ LCCCM = 1∶ 5∶ 5∶ 10∶ 67∶ 12, achieving a 28-day compressive strength of 0.70 MPa, which complies with the engineering requirements specified in the standard. Furthermore, micro-characterization technology techniques, including scanning electron microscopy (SEM) and X-ray diffraction (XRD), demonstrate that: (1) the multi-component materials form a densely packed structure, significantly enhancing mechanical properties; and (2) hydration products effectively fill pores, dramatically improving material durability. These findings provide theoretical guidance for the design and development of low-carbon flowable solidified soils.
The synergistic resource utilization of solid waste represents a critical scientific challenge in the field of environmental engineering. This study innovatively proposes a solid waste-based fluid-filled material (FFM) to address the significant physicochemical differences among multi-source solid wastes, including drinking water treatment residues (DWTR), mixing station sediments (MSS), recycled fine aggregates (RFA), and mud. The results demonstrate that by precisely controlling the fluidity (160–200 mm), water-to-solid ratio (0.52–0.87), and wet density (1.47–1.68 g/cm 3 ) of the material, FFM with excellent mechanical properties can be obtained, exhibiting 7-day and 28-day compressive strengths of 0.20–1.20 MPa and 0.40–1.40 MPa, respectively. Under the premise of meeting performance requirements, the mechanical properties of FFM significantly improve with increasing proportions of low-carbon composite cementitious material (LCCCM) and RFA, as well as with extended curing age. Through systematic optimization, the optimal mix ratio for FFM was determined as Lime∶ DWTR∶ MSS∶ RFA∶ mud∶ LCCCM = 1∶ 5∶ 5∶ 10∶ 67∶ 12, achieving a 28-day compressive strength of 0.70 MPa, which complies with the engineering requirements specified in the standard. Furthermore, micro-characterization technology techniques, including scanning electron microscopy (SEM) and X-ray diffraction (XRD), demonstrate that: (1) the multi-component materials form a densely packed structure, significantly enhancing mechanical properties; and (2) hydration products effectively fill pores, dramatically improving material durability. These findings provide theoretical guidance for the design and development of low-carbon flowable solidified soils.
期刊：
Journal of Inflammation Research,2025年18:5727-5739 ISSN：1178-7031
通讯作者：
He, J;Deng, Zhongliang
作者机构：
[Li, Yuan; He, Jun; Yang, Xue; Li, Chao; Huang, Ying; Liao, Daoyong] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang, Peoples R China.;[Deng, Zhongliang; He, Jun; Yang, Xue] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang, Hunan, Peoples R China.
通讯机构：
[He, J ; Deng, ZL] U;Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Clin Lab, Hengyang, Peoples R China.;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang, Hunan, Peoples R China.
关键词：
M. pneumoniae;CCL20;EMT;AKT-ERK1/2-AP1;cell migration
摘要：
PURPOSE: Mycoplasma pneumoniae, a clinically significant respiratory pathogen, primarily causes community-acquired pneumonia and contributes to asthma development, with its persistent infection frequently resulting in fibrotic pulmonary changes and structural airway abnormalities. This study investigates the signaling pathways regulating CCL20 expression in THP-1 cells following M. pneumoniae infection and its impact on cell migration and epithelial-mesenchymal transition (EMT). METHODS: THP-1 cells were infected with M. pneumoniae, and the expression of CCL20 was measured over time and at various doses. In addition, co-culture experiments were performed using M. pneumoniae-infected THP-1 cells and bronchial epithelial cells to assess EMT and cell migration. RESULTS: M. pneumoniae infection significantly upregulated CCL20 production in THP-1 cells via the AKT-ERK1/2-AP1 pathway, a process that was both time- and dose-dependent. Furthermore, co-culturing M. pneumoniae-infected THP-1 cells with 16HBE cells promoted EMT and increased cell migration, a process that is believed to be associated with CCL20. CONCLUSION: This study provides insights into the molecular mechanisms linking CCL20 to cell migration, highlighting potential therapeutic targets for M. pneumoniae-related lung diseases.
摘要：
Gestational diabetes mellitus (GDM) is one of the most widespread obstetric complications, and its prevalence increases year by year. GDM raises the risk of perinatal maternal and fetal complications, such as gestational hypertension and preeclampsia, and even affects the long-term health of the fetus and child. It is well known that trace elements are important for the health of pregnant women. Recent studies have demonstrated a link between GDM and changes in trace element levels in the body. Moreover, the theory of developmental origins of health and disease emphasizes the significance of the intrauterine environment during pregnancy for the long-term health of the fetus. Previous studies have reported that changes in the intrauterine environment increase the risk of neurodevelopmental disorders. However, the mechanisms involved have not been elucidated. Here, we summarize the potential mechanisms behind GDM and neurodevelopmental problems in offspring. In addition, the effects of trace elements zinc, selenium, and chromium are explored in modulating GDM and their potentially beneficial effects in improving neurodevelopment, which might provide new insights into the potential molecular mechanisms underlying the effects of maternal hyperglycemia on the neurodevelopment of the offspring and the use of trace elements to improve neurodevelopment in offspring of individuals who experienced GDM.
期刊：
Chemical Communications,2025年61(18):3756-3759 ISSN：1359-7345
通讯作者：
Pan, Jiafeng;Yang, F
作者机构：
[Wang, Yuyan; Yang, F; Pan, Jiafeng; Zeng, Ying; Ren, Xiaoya; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Qiu, Jun] Hunan Childrens Hosp, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Yang, F ; Pan, JF] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词：
Biosensing Techniques;DNA, Catalytic;Fluorescence;Fluorescent Dyes;Limit of Detection;Spectrometry, Fluorescence;Uranium Compounds;Biosensors;atrazine;chemical compound;deoxyribozyme;enzyme;gold nanoparticle;penicillin derivative;thiamethoxam;unclassified drug;uranyl ion;deoxyribozyme;fluorescent dye;uranium derivative;uranium dioxide;DNAzyme;Fluorescence biosensors;Fluorescence signals;Signal amplifiers;UO 2;Water samples;Y-shaped;Article;diagnostic test accuracy study;enzyme activity;human;sensitivity and specificity;walking;chemistry;fluorescence;genetic procedures;limit of detection;metabolism;spectrofluorometry;Wheels
摘要：
Based on a Y-shaped wheel-mediated triple walker, an enzyme-free biosensor was reported for UO22+ detection. Due to the DNAzyme-driven mechanism, our walker was activated and produced a fluorescence signal for UO22+ assay. The sensor demonstrated ultra-sensitivity, good specificity and excellent accuracy, holding great promise for UO22+ sensing in complex water samples.
摘要：
A sensitive fluorescence biosensor was developed for microcystin-LR (MC-LR) detection using H1, H2, and H3 DNA probes as sensing elements. The aptamer in H1 can recognize the target. H2 was labeled with FAM and BHQ. The MC-LR and H1 binding will activate the H2 and H3 self-assemblies through toehold-mediated strand displacement. In the formed products (MC-LR/H1/nH2/nH3), FAM and BHQ will be separated and a high FAM fluorescence signal can be observed for the MC-LR assay. The biosensor is sensitive with a detection limit of 53 fM. We further constructed several logic circuits (AND-AND cascaded circuit, feedforward circuit, and resource allocation circuit) using MC-LR, MC-LA, and MC-YR as the three inputs. The numbers 0 and 1 are used to code the input and output signals. The AND-AND cascade circuit can produce a high output signal only in the (111) input combination. In the feedforward circuit, MC-LR and MC-LA can activate the logic circuit to produce high signals, and MC-YR will inhibit the self-assembly and execute the negative feedforward operation. Through the rational design of the DNA probe hybridizations on four different magnetic beads (MBs), the resource allocation circuit can achieve an intelligent allocation of input information. Our proposed fluorescence biosensor can not only provide a sensitive platform for microcystin detection but also serve as a smart and intelligent logic system for microcystin sensing.
摘要：
Photocatalysis holds promise for extracting uranium from aqueous solution. Nevertheless, conventional approaches generally rely on sacrificial agents and anaerobic conditions to maintain photocatalytic efficiency, which increases costs and causes secondary pollution. Herein, we introduce the design and synthesis of an Sscheme ZnIn2S4/g-C3N4 (ZISCN) heterojunction photocatalyst for the efficient removal of uranium via in-situ generating ZnIn2S4 on g-C3N4. Photoelectric characterization and theoretical calculation indicate that ZISCN boosts the absorption of visible light and promotes the effective separation and migration of charge carriers by forming an internal electric field (IEF) at the S-scheme heterojunction interface. This configuration integrates the strong reducing electrons of g-C3N4 and the potent oxidation holes of ZnIn2S4. Consequently, the as-synthesized ZISCN can efficiently remove uranium under an air atmosphere without the need for sacrificial agents and anaerobic conditions. The achieved U(VI) removal rate of 94.8 % surpasses that of ZnIn2S4 and g-C3N4 individually. Moreover, the photocatalytic extraction of U(VI) by ZISCN photocatalyst demonstrated excellent stability and anti-interference performance. After five cycles, the U(VI) removal rate remained above 85 %. Mechanism studies reveal that when electrons are generated by light in the ZISCN systems, they can reduce O2, leading to the formation of reactive species & sdot;O2/H2O2. These species subsequently interact with U(VI), resulting in the precipitation of (UO2)O2 & sdot;2H2O on the surface of ZISCN. This research provides valuable insights for the design of heterojunction photocatalysts for efficient, sacrificial agent-free uranium removal in ambient air environments.
