Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhu, Wenxuan;Zhao, Shuhua;Yu, Bei;Tao, Yucun;Wang, Chaoyang;...

通讯作者：

Wu, CQ;Ding, Chunguang;Yu, LJ

作者机构：

[Wu, CQ; Wu, Chengqiu; Zhu, Wenxuan; Yu, Bei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.

[Ding, Chunguang; Meng, Jing; Zhao, Shuhua; Yu, Lijia; Zhu, Wenxuan; Yu, Bei] NHC, Natl Ctr Occupat Safety & Hlth, NHC Key Lab Engn Control Dust Hazard, Beijing 102308, Peoples R China.

[Zhao, Shuhua; Zhang, Xiufeng; Shi, Lei] North China Univ Sci & Technol, Tangshan 063210, Peoples R China.

[Tao, Yucun] Shantou Univ, Sch Publ Hlth, Shantou 515041, Peoples R China.

[Wang, Chaoyang] Third Peoples Hosp Henan Prov, Key Med Lab Chem Poison Detect Henan Prov, Zhengzhou, Peoples R China.

通讯机构：

[Wu, CQ ] U

[Ding, CG; Yu, LJ ] N

Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.

NHC, Natl Ctr Occupat Safety & Hlth, NHC Key Lab Engn Control Dust Hazard, Beijing 102308, Peoples R China.

语种：

英文

关键词：

Squaraine dyes;Pyrophosphate;Alkaline phosphatase;Detection

期刊：

Analytical Sciences

ISSN：

0910-6340

年：

2025

卷：

期：

页码：

289-296

DOI：

10.1007/s44211-024-00697-2

基金类别：

The Key Medical Laboratory for Chemical Poison Detection of Henan Province#&#&#HNHDJFK202404 Open Project of Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation#&#&#BMRE 2022-KF04 the key project of the National Center of Occupational Safety and Health, National Health Commission of the Peoples Republic of China#&#&#ZY-2023-ZD 06-01#&#&#2021-ZD-8)

机构署名：

本校为第一且通讯机构

院系归属：

公共卫生学院

摘要：

We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu(2+) ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu(2+). Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an "off-on-off" fluorescence response. The detection limits...

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Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase

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