A ERA/Cas12f1_ge4.1 biosensor for rapid, sensitive, and cost-effective detection of Chlamydia psittaci via fluorescence and lateral flow assays

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成果类型：

期刊论文

作者：

Jiang, Tingqing;Wei, Guijiang;Lin, Mei;Zhang, Sheping;Zou, Lintao;...

通讯作者：

Deng, ZL

作者机构：

[Jiang, Tingqing] Xiangnan Univ, Affiliated Hosp, Chenzhou 423099, Peoples R China.

[Deng, ZL; Lin, Mei; Deng, Zhongliang] Hunan Univ Med, Sch Med Lab, Changsha 418000, Hunan, Peoples R China.

[Wei, Guijiang] Youjiang Med Univ Nationalities, Affiliated Hosp, Ctr Med Lab Sci, Baise 533000, Guangxi, Peoples R China.

[Wei, Guijiang] Baise Key Lab Res & Dev Clin Mol Diag High Inciden, Baise 533000, Guangxi, Peoples R China.

[Zou, Lintao; Zhou, Xuan] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.

通讯机构：

[Deng, ZL ] H

Hunan Univ Med, Sch Med Lab, Changsha 418000, Hunan, Peoples R China.

语种：

英文

关键词：

Cas12f1_ge4.1;Chlamydia psittaci;Enzymatic recombinase amplification (ERA);Field-deployable diagnostics;Fluorescence;Lateral flow assay

期刊：

Talanta

ISSN：

0039-9140

年：

2025

卷：

287

页码：

127615

DOI：

10.1016/j.talanta.2025.127615

基金类别：

CRediT authorship contribution statement Tingqing Jiang: Writing – review & editing, Software, Methodology, Data curation. Guijiang Wei: Writing – review & editing, Investigation, Data curation. Mei Lin: Resources, Methodology, Data curation. Sheping Zhang: Writing – review & editing, Validation, Data curation. Lintao Zou: Supervision, Methodology, Data curation. Xuan Zhou: Methodology, Data curation. Zhongliang Deng: Writing – review & editing, Visualization, Supervision, Resources, acquisition, Conceptualization.

机构署名：

本校为其他机构

院系归属：

公共卫生学院

摘要：

Rapid and accurate detection of Chlamydia psittaci , the causative agent of psittacosis, is crucial for both human and animal health but presents significant challenges, particularly in grassroots health institutions. Our previous PDTCTR fluorescence sensing platform, which combined the engineered Cas12f1_ge4.1 system with recombinase polymerase amplification (RPA), significantly enhanced detection efficiency. However, its requirement for specialized equipment, costly RPA reagents, and absence of visual output restricted its practical applicati...

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A ERA/Cas12f1_ge4.1 biosensor for rapid, sensitive, and cost-effective detection of Chlamydia psittaci via fluorescence and lateral flow assays

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