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Dynamic synthesis and transport of phenazine-1-carboxylic acid to boost extracellular electron transfer rate

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成果类型:
期刊论文
作者:
"Li, Feng;Zhang, Baocai;Long, Xizi;Yu, Huan;Shi, Sicheng;...
通讯作者:
Hao Song
作者机构:
[Li, Yuanxiu; Shi, Sicheng; Liu, Qijing; "Li, Feng; Yu, Huan; Wu, Shengbo; An, Xingjuan; Zhang, Baocai; Li, Chao; You, Zixuan; Tang, Rui] State Key Laboratory of Synthetic Biology, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China
[Yu, Huan; Zhang, Baocai] College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China
[Long, Xizi] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China
[Shi, Liang] Department of Biological Sciences and Technology, School of Environmental Studies, China University of Geoscience in Wuhan, Wuhan, Hubei, 430074, China
[Nealson, Kenneth H.] Departments of Earth Science & Biological Sciences, University of Southern California, 4953 Harriman Ave., South Pasadena, CA, 91030, USA
通讯机构:
[Hao Song] S
State Key Laboratory of Synthetic Biology, and School of Chemical Engineering and Technology, Tianjin University, Tianjin, China<&wdkj&>College of Life and Health Sciences, Northeastern University, Shenyang, China
语种:
英文
期刊:
Nature Communications
ISSN:
2041-1723
年:
2025
卷:
16
期:
1
页码:
2882
机构署名:
本校为其他机构
院系归属:
公共卫生学院
摘要:
Electron shuttle plays a decisive role in extracellular electron transfer (EET) of exoelectrogens. However, neither identifying the most efficient electron shuttle molecule nor programming its optimal synthesis level that boosts EET has been established. Here, the phenazine-1-carboxylic acid (PCA) biosynthesis pathway is first constructed to synthesize PCA at an optimal level for EET in Shewanella oneidensis MR-1. To facilitate PCA transport, the porin OprF is expressed to improve cell membrane permeability, the cytotoxicity of which, however, ...

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