摘要：
BACKGROUND: Antibiotics, as the most commonly prescribed class of drugs in neonatal intensive care units, have an important impact on the developing neonatal gut microbiota. Therefore, comprehending the effects of commonly used antibiotic therapy on the gut microbiota and butyrate-producers in early infants could provide information for therapeutic decision-making in the NICU. OBJECTIVES: To explore the effects of antibiotic therapy on the early development of gut microbiota and butyrate-producers in early infants. METHODS: A total of 72 infants were included in the study. We performed 16S rRNA sequencing on stool swab samples collected from neonatal intensive care unit patients who received amoxicillin-clavulanic acid (AC, n = 10), moxalactam (ML, n = 28) and non-antibiotics (NA, n = 34). We then compared the taxonomic composition between treatment regimens, focusing on differences in butyrate-producers. RESULTS: Our study showed that there were significant differences in Shannon index (p = 0.033) and Beta diversity (p = 0.014) among the three groups. At the family level, compared with the other two groups, the relative abundance of Clostridiaceae (p < 0.001) and Veillonellaceae (p = 0.004) were significantly higher, while the relative abundance of Enterococcidae (p < 0.001) was significantly lower in the NA group. The relative abundance of Enterobacteriaceae (p = 0.022) in the AC group was greater than that in the other two groups. Additionally, butyrate-producers (p < 0.001), especially Clostridiaceae (p < 0.001), were noticeably more abundant in the NA group. The relative abundance of Clostridiaceae and butyrate-producers were the lowest in the ML group (p < 0.001). CONCLUSION: We found that antibiotic therapy had an adverse impact on the initial development of gut microbiota and leaded to a reduction in the abundance of butyrate-producers, particularly Clostridiaceae. Furthermore, moxalactam had a more pronounced effect on the gut microbiota compared to amoxicillin-clavulanic acid.
摘要：
Occupational exposure to N-hexane/2,5-hexanedione (2,5-HD) was found to adversely affect reproductive functions in females. However, there are few studies regarding the mechanisms underlying reproductive system damage initiated by 2,5-HD. Several studies demonstrated that 2,5-HD exerts hormonal dysfunctions in females by promoting apoptosis using rat ovarian granulosa cells (GCs) as a model. The endoplasmic reticulum (ER) plays a key role in cellular processes such as protein folding and modification, Ca(2+) storage, and lipid synthesis, which are known to involve the activation of stress (ERS)-dependent m-TOR signaling pathway. Thus, the aim of this study was to examine the effects of 2,5-HD on ER and the associated activation of stress (ERS)-dependent m-TOR signaling pathway resulting in consequent apoptosis of ovarian GCs. Data demonstrated that after intraperitoneal treatment with 100, 200, or 400 mg/kg 2,5-HD for 6 consecutive weeks, 5 times per week, a decrease in body weight, ovarian weight, and relative ovary weight was found. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay showed that 2,5-HD promoted apoptosis of ovarian GCs, which involved enhanced relative protein expression levels of m-TOR/p-mTOR. Our findings demonstrated that 2,5-HD (1) elevated expression levels of pro-apoptosis-related genes Bax and Caspase 3, (2) decreased expression levels of the anti-apoptosis gene Bcl-2, and (3) activated the protein expression of glucose-regulatory protein 78 (GRP78), inositol-requiring enzyme-1 (IRE1), and c-Jun terminal kinase (JNK) associated with increased apoptosis. Evidence indicates that chronic exposure to 2,5-HD induced apoptosis of ovarian GCs, and the possible mechanism underlying this effect involves the ERS-dependent m-TOR signaling pathway.
作者机构：
[Zhao, Kunyan; Song, Fengmei; Yang, Fei; Tang, Yan] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Wu, Mingyang] Cent South Univ, Xiangya Sch Publ Hlth, Dept Maternal & Child Hlth, Changsha 410007, Peoples R China.;[Qiu, Jun; Qiu, J; Pan, Xiongfeng; Liu, Caixia; Xiang, Shiting] Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Pediat Res Inst Hunan Prov,Xiangya Sch Med, Changsha 410007, Peoples R China.;[Peng, Yunlong] Soochow Univ, Dept Epidemiol & Hlth Stat, Med Coll, Suzhou 215123, Peoples R China.;[Cao, Yunhui; Wu, Sha] Univ South China, Hengyang Med Sch, Dept Pediat, Hengyang 421001, Peoples R China.
通讯机构：
[Qiu, J ] C;Cent South Univ, Affiliated Childrens Hosp, Hunan Childrens Hosp, Pediat Res Inst Hunan Prov,Xiangya Sch Med, Changsha 410007, Peoples R China.
关键词：
Early life;Gut microbiota;Neonate;Thallium
摘要：
Previous research has found a correlation between heavy metals and gut microbiota in humans. However, there are few population-based studies examining the impact of early life thallium (Tl) exposure on neonatal microbiome. 342 newborns were recruited from Hunan Children's Hospital and subsequently divided into three groups (low, medium, and high) based on the 25th and 75th percentiles of serum Tl concentration. Additionally, the relationship between Tl and gut microbiota was analyzed in subgroups (preterm or full-term neonates). The association between Tl and gut microbiota in neonates was analyzed by Redundancy analysis, Spearman correlation analysis and MaAsLin2. The detection rate of Tl in neonates was 100%, with the median concentration of 0.021 μg/L. In all neonates, we found significant differences in the Chao1 and ACE indices of α-diversity in gut microbiota, and the relative abundances of Bacteroidota and Bacteroidetes were significantly different among groups ( p < 0.05). Following the covariate adjustment, Tl was negatively correlated with Gemmatimonadota (Coef = 0.265, p < 0.05) in preterm neonates. In full-term neonates, Tl exhibited a positive correlation with the relative abundance of Robinsoniella (Coef = 0.563, p = 0.009) and a negative correlation with that of Pseudomonas (Coef = - 0.592, p = 0.012). Tryptophan and renin-angiotensin system pathways might exert important roles in Tl exposure. This study indicated that Tl exposure was associated with changes in α-diversity and the composition of gut microbiota in neonates, with Gemmatimonadota being predominantly affected in preterm neonates and Robinsoniella and Pseudomonas in full-term neonates.
Previous research has found a correlation between heavy metals and gut microbiota in humans. However, there are few population-based studies examining the impact of early life thallium (Tl) exposure on neonatal microbiome. 342 newborns were recruited from Hunan Children's Hospital and subsequently divided into three groups (low, medium, and high) based on the 25th and 75th percentiles of serum Tl concentration. Additionally, the relationship between Tl and gut microbiota was analyzed in subgroups (preterm or full-term neonates). The association between Tl and gut microbiota in neonates was analyzed by Redundancy analysis, Spearman correlation analysis and MaAsLin2. The detection rate of Tl in neonates was 100%, with the median concentration of 0.021 μg/L. In all neonates, we found significant differences in the Chao1 and ACE indices of α-diversity in gut microbiota, and the relative abundances of Bacteroidota and Bacteroidetes were significantly different among groups ( p < 0.05). Following the covariate adjustment, Tl was negatively correlated with Gemmatimonadota (Coef = 0.265, p < 0.05) in preterm neonates. In full-term neonates, Tl exhibited a positive correlation with the relative abundance of Robinsoniella (Coef = 0.563, p = 0.009) and a negative correlation with that of Pseudomonas (Coef = - 0.592, p = 0.012). Tryptophan and renin-angiotensin system pathways might exert important roles in Tl exposure. This study indicated that Tl exposure was associated with changes in α-diversity and the composition of gut microbiota in neonates, with Gemmatimonadota being predominantly affected in preterm neonates and Robinsoniella and Pseudomonas in full-term neonates.
作者机构：
[Liu, Yu; Long, Dingxin; Ning, Yujun; Zhang, Zhibo; Wang, Ru; Zhong, Chiting; Zhao, Weichao; Zhao, WC; Wang, Kongfan; Chen, Xiaobing; Ou, Yiquan] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Ou, Yiquan] Chenzhou Peoples Hosp 1, Dept Nutr, Chenzhou 423000, Peoples R China.;[Jiang, Yi] Sichuan Univ, West China Sch Publ Hlth, Chengdu 610041, Peoples R China.;[Jiang, Yi] Sichuan Univ, West China Hosp 4, Chengdu 610041, Peoples R China.;[Zhang, Zhibo] Univ South China, Hengyang Med Sch, Clin Fac 1, Hengyang 421001, Peoples R China.
通讯机构：
[Long, DX; Zhao, WC ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
关键词：
Growth and development;Neurobehavior;Oxygenated polycyclic aromatic hydrocarbons (OPAHs);Polyethylene
摘要：
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of anthropogenic, persistent, and highly toxic PAH contaminants associated with developmental toxicity, 9-fluorenone (9-FLO) is a typical member of the OPAH family. Due to its ketone group, it has higher polarity, which results in increased solubility in water and greater potential for transport via atmospheric particles or water bodies. Polyethylene (PE), an amorphous polymer, is characterized by high diffusivity, high permeability, and a large internal molecular free volume, which confers a strong absorption capacity for organic pollutants. The effects of individual and combined exposures to these two common environmental pollutants on aquatic life remain unclear. In this study, we evaluated the effects of PE and 9-FLO exposure on growth, development, metabolism, and behavior using zebrafish as a model organism. We employed methods and techniques such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking, automated behavior analysis, microscopy imaging, and real-time fluorescence quantification. Zebrafish embryos at 2 hours post-fertilization (hpf) were exposed to PE and 9-FLO, both individually and in combination. Our studies showed that exposure to PE or 9-FLO alone increases embryonic mortality and decreases hatchability compared to the control group. The 9-FLO group exhibited delayed hatching and inhibited larval length growth. The exposed groups showed a loose arrangement of telencephalic neurons, partial apoptosis, decreased dopamine (DA) content, increased serotonin (5-HT) content, decreased exercise capacity, reduced rhythmic amplitude, and increased rest time. The combined exposure group showed a slight alleviation of these effects compared to the single exposure groups but still exhibited significant differences from the control group. In summary, early exposure to PE and 9-FLO in zebrafish embryos, whether alone or in combination, affects growth, development, apoptosis, neurotransmitter release, and motor behavior of zebrafish neurons.
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of anthropogenic, persistent, and highly toxic PAH contaminants associated with developmental toxicity, 9-fluorenone (9-FLO) is a typical member of the OPAH family. Due to its ketone group, it has higher polarity, which results in increased solubility in water and greater potential for transport via atmospheric particles or water bodies. Polyethylene (PE), an amorphous polymer, is characterized by high diffusivity, high permeability, and a large internal molecular free volume, which confers a strong absorption capacity for organic pollutants. The effects of individual and combined exposures to these two common environmental pollutants on aquatic life remain unclear. In this study, we evaluated the effects of PE and 9-FLO exposure on growth, development, metabolism, and behavior using zebrafish as a model organism. We employed methods and techniques such as acridine orange staining, enzyme-linked immunosorbent assay (ELISA), video tracking, automated behavior analysis, microscopy imaging, and real-time fluorescence quantification. Zebrafish embryos at 2 hours post-fertilization (hpf) were exposed to PE and 9-FLO, both individually and in combination. Our studies showed that exposure to PE or 9-FLO alone increases embryonic mortality and decreases hatchability compared to the control group. The 9-FLO group exhibited delayed hatching and inhibited larval length growth. The exposed groups showed a loose arrangement of telencephalic neurons, partial apoptosis, decreased dopamine (DA) content, increased serotonin (5-HT) content, decreased exercise capacity, reduced rhythmic amplitude, and increased rest time. The combined exposure group showed a slight alleviation of these effects compared to the single exposure groups but still exhibited significant differences from the control group. In summary, early exposure to PE and 9-FLO in zebrafish embryos, whether alone or in combination, affects growth, development, apoptosis, neurotransmitter release, and motor behavior of zebrafish neurons.
摘要：
Lactic acid has aroused increasing attention due to its close association with serious diseases. A real-time, dynamic, and intelligent detection method is vital for sensitive detection of lactic acid. Here, a machine learning (ML)-assisted perspiration-driven self-powered sensor (PDS sensor) is fabricated using Ni-ZIF-8@lactate oxidase and pyruvate oxidase (Ni-ZIF-8@LOx&POx)/laser-induced graphene (LIG), bilirubin oxidase (BOD)/LIG, and a microchannel for highly sensitive and real-time monitoring of lactic acid in sweat. Driven by the oxidation reaction of lactic acid, PDS sensors exhibit excellent sensitivity, a wide detection range, good reproducibility, and excellent selectivity for lactic acid detection in sweat. When subjects with different body mass index (BMI) undergo aerobic or anaerobic exercise or maintain a sedentary state, PDS sensors can monitor lactic acid in sweat wirelessly and in real-time. Moreover, a ML algorithm was employed to assist PDS sensors to detect lactic acid in the subjects' sweat with a high prediction accuracy of 96.0%.
摘要：
Beryllium (Be) is a recognised environmental toxicant associated with pulmonary fibrosis. Epithelial-mesenchymal transition (EMT), a critical process in cell phenotype conversion, plays a key role in its pathophysiology. Methyltransferase-like 3 (METTL3), a major N6-methyladenosine methyltransferase, regulates gene expression and cellular functions. However, its role in Be-induced EMT remains unclear. In this study, human bronchial epithelial cell line (16HBE cells) were exposed to varying concentrations of beryllium sulphate (BeSO(4)) to assess changes in METTL3 expression. METTL3 overexpression vectors were constructed, and quantitative reverse transcription-polymerase chain reaction, western blotting and immunofluorescence were used to detect METTL3, EMT markers and Wingless/Integrated (Wnt)/β-catenin pathway proteins. The Wnt/β-catenin pathway inhibitor ICG-001 was also employed to explore the role of the Wnt/β-catenin pathway in BeSO(4)-induced EMT. The study demonstrated that BeSO(4) suppressed METTL3 expression, induced EMT and activated the Wnt/β-catenin pathway in 16HBE cells. Both METTL3 overexpression and ICG-001 pretreatment mitigated BeSO(4)-induced EMT and Wnt/β-catenin pathway activation. These findings suggest that METTL3 inhibits BeSO(4)-induced EMT by suppressing the Wnt/β-catenin pathway, offering novel mechanistic insights into beryllium toxicity and a potential therapeutic target for Be-related pulmonary fibrosis.
关键词：
Aptasensor;Dose assessment;Electrochemical impedance aptasensor;Radiation biodosimeters;p21 protein
摘要：
Radiation dose assessment is the main basis for the diagnosis of acute radiation sickness. At present, there is a lack of rapid and portable dose assessment methods, which has an important impact on the rapid diagnosis and precise treatment of radiation accident patients and nuclear practitioners. We selected and obtained specific aptamers for radiation-sensitive protein p21 protein by the magnetic cross-linking precipitation (MCP)-SELEX procedure. The aptamer has a high affinity for binding to the p21 protein and its K d value is 2.21×10 -7 mol/L. We subsequently established a new method for radiation dose assessment of an electrochemical impedance (EIS) aptasensor with screen-printed electrode chips. There was a good dose-effect relationship between the p21 protein expression level in PBMCs in human peripheral blood detected by this method within the dose range of 0-10 Gy, and detection limit of radiation dose is 0.38 Gy (LOD, S/N = 3). This dose range covers the diagnostic range of acute radiation sickness in the bone marrow. This method is not only portable but also fast, saving hours to days compared with the previous dose assessment method based on radiation sensitive protein. It can be applied to the rapid and portable diagnosis of acute radiation sickness.
Radiation dose assessment is the main basis for the diagnosis of acute radiation sickness. At present, there is a lack of rapid and portable dose assessment methods, which has an important impact on the rapid diagnosis and precise treatment of radiation accident patients and nuclear practitioners. We selected and obtained specific aptamers for radiation-sensitive protein p21 protein by the magnetic cross-linking precipitation (MCP)-SELEX procedure. The aptamer has a high affinity for binding to the p21 protein and its K d value is 2.21×10 -7 mol/L. We subsequently established a new method for radiation dose assessment of an electrochemical impedance (EIS) aptasensor with screen-printed electrode chips. There was a good dose-effect relationship between the p21 protein expression level in PBMCs in human peripheral blood detected by this method within the dose range of 0-10 Gy, and detection limit of radiation dose is 0.38 Gy (LOD, S/N = 3). This dose range covers the diagnostic range of acute radiation sickness in the bone marrow. This method is not only portable but also fast, saving hours to days compared with the previous dose assessment method based on radiation sensitive protein. It can be applied to the rapid and portable diagnosis of acute radiation sickness.
期刊：
Journal of Environmental Management,2025年387:125912 ISSN：0301-4797
通讯作者：
Xiaojun Li
作者机构：
[Jia Wei; Jiayou Luo; Zhihang Huang; Miyang Luo] Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, 410078, China;[Yanhua Chen; Fei Wang; Xiaojun Li] Department of School Health, Hunan Provincial Center for Disease Control and Prevention, Changsha, 410005, China;[Fei Yang; Yamei Li; Zhaozhong Zhu] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang, 421001, China
通讯机构：
[Xiaojun Li] D;Department of School Health, Hunan Provincial Center for Disease Control and Prevention, Changsha, 410005, China
摘要：
Air pollution has emerged as an essential risk factor for overweight and obesity. However, the combined effects of multiple air pollutants on overweight/obesity development in children and adolescents are not fully understood. In this study, a total of 189,448 children and adolescents in China were included. Logistics, weighted quantile sum, quantile g-computation, and bayesian kernel machine regression models were used to systematically assess the association between long-term outdoor air pollution exposure and overweight/obesity, and identified the major contributors. Our results revealed a significantly positive association of PM 2.5 , PM 10 , CO, and NO 2 concentrations with overweight/obesity risk. Multi-pollutant models consistently demonstrated a positive association between the air pollutant mixture and the risk of overweight/obesity (OR: 1.825; 95 % CI: 1.036, 2.614). PM 2.5 and PM 10 were identified as the most significant contributors. Furthermore, we found significantly positive overall effects and interactions of these pollutants on an additive risk of overweight/obesity. The effects of air pollutants on overweight/obesity were pronounced in boys, rural residents, smokers, and primary school students. Our findings demonstrated that long-term exposure to air pollutants, particularly PM 2.5 and PM 10 was positively linked with an increased risk of overweight/obesity in children and adolescents. The cross-sectional design and potential confounders limited the ability to establish causality. Prospective cohort studies and specific mechanism investigations are needed to provide more precise and robust evaluations in the future. Coordinated policies to reduce air pollutants and mitigate their combined effects are essential for addressing this public health issue.
Air pollution has emerged as an essential risk factor for overweight and obesity. However, the combined effects of multiple air pollutants on overweight/obesity development in children and adolescents are not fully understood. In this study, a total of 189,448 children and adolescents in China were included. Logistics, weighted quantile sum, quantile g-computation, and bayesian kernel machine regression models were used to systematically assess the association between long-term outdoor air pollution exposure and overweight/obesity, and identified the major contributors. Our results revealed a significantly positive association of PM 2.5 , PM 10 , CO, and NO 2 concentrations with overweight/obesity risk. Multi-pollutant models consistently demonstrated a positive association between the air pollutant mixture and the risk of overweight/obesity (OR: 1.825; 95 % CI: 1.036, 2.614). PM 2.5 and PM 10 were identified as the most significant contributors. Furthermore, we found significantly positive overall effects and interactions of these pollutants on an additive risk of overweight/obesity. The effects of air pollutants on overweight/obesity were pronounced in boys, rural residents, smokers, and primary school students. Our findings demonstrated that long-term exposure to air pollutants, particularly PM 2.5 and PM 10 was positively linked with an increased risk of overweight/obesity in children and adolescents. The cross-sectional design and potential confounders limited the ability to establish causality. Prospective cohort studies and specific mechanism investigations are needed to provide more precise and robust evaluations in the future. Coordinated policies to reduce air pollutants and mitigate their combined effects are essential for addressing this public health issue.
期刊：
Journal of Nanobiotechnology,2025年23(1):1-15 ISSN：1477-3155
通讯作者：
Du, Meng;Chen, ZY
作者机构：
[Lei, Lingling; Du, Meng; Chen, Zhiyi; Li, Mingjie; Du, M; Xu, Haonan] Univ South China, Affiliated Changsha Cent Hosp, Coll Hunan Prov, Hengyang Med Sch,Key Lab Med Imaging Precis Theran, Changsha, Peoples R China.;[Lei, Lingling; Du, Meng; Chen, Zhiyi; Li, Mingjie; Du, M] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Med Imaging, Changsha, Peoples R China.;[Lei, Lingling; Du, Meng; Chen, Zhiyi; Li, Mingjie; Du, M; Xu, Haonan] Univ South China, Inst Med Imaging, Hengyang Med Sch, Hengyang, Peoples R China.;[Xu, Haonan] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.
通讯机构：
[Chen, ZY ; Du, M] U;Univ South China, Affiliated Changsha Cent Hosp, Coll Hunan Prov, Hengyang Med Sch,Key Lab Med Imaging Precis Theran, Changsha, Peoples R China.;Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Med Imaging, Changsha, Peoples R China.;Univ South China, Inst Med Imaging, Hengyang Med Sch, Hengyang, Peoples R China.
摘要：
BACKGROUND: Radiotherapy efficacy remains constrained by two key challenges: dose-dependent toxicity to healthy tissues at high radiation doses and hypoxia-mediated tumor radioresistance. While radiosensitizers like gold nanoparticles can enhance tumor-specific radiation deposition, their targeted delivery to tumors presents a significant hurdle. Bacteria have emerged as promising bio-carriers that not only actively target tumors and penetrate complex microenvironments, but can also be genetically engineered as multifunctional platforms for radiosensitizer delivery and hypoxia alleviation. RESULTS: An integrated nanosystem (PCM@AuNPs), composed of engineered bacteria (PCM) and gold nanoparticles (AuNPs), is used to increase the effectiveness of radiotherapy. PCM can target and colonize tumor sites more effectively, thus improving the delivery efficiency of radiosensitizers. Furthermore, PCM overexpresses catalase (CAT), which decomposes excess H(2)O(2) into O(2), helping to mitigate hypoxia in the TME. Under X-ray irradiation, PCM@AuNPs significantly enhance radiosensitization, leading to improved tumor growth inhibition while maintaining good biocompatibility. CONCLUSIONS: An effective strategy based on an integrated nanosystem (PCM@AuNPs) for radiosensitization through multiple pathways is developed. This novel engineered bacterial strategy holds great promise for enhancing radiosensitization in cancer therapy.
摘要：
Uranium is the core material for the development of the nuclear industry, but its irreversible radiation damage poses a significant threat to human health. In this context, an innovative dual-mode colorimetric and electrochemical sensor was developed for the detection of uranyl ions (UO(2)(2+)), utilizing a covalent organic framework@gold nanoclusters (AuNCs@COF) composite. The synthesis of AuNCs@COF was simple, and the incorporation of AuNCs imparted the composite with exceptional peroxidase-like catalytic activity and enhanced electrochemical properties. By regulating the adsorption and desorption of aptamers on the AuNCs@COF surface, both peroxidase-like activity and conductivity were modulated, enabling the detection of UO(2)(2+) utilizing colorimetric and electrochemical dual signals. Under optimal conditions, the sensor revealed a broad linear detection range and a low detection limit, with ranges of 1.36 × 10(-10)-1.36 × 10(-5)mol/L for colorimetric detection and 5.0 × 10(-10)-2.5 × 10(-5)mol/L for electrochemical detection, achieving detection limitsfor these two methodsof 107 pmol/L and 347 pmol/L, respectively. Unlike other single-mode sensorsfor UO(2)(2+) detection, this dual-mode sensor demonstrated superior sensitivity, specificity, and repeatability. Furthermore, the results of spiked recovery experiments in real water samples highlight the promising potential of this dual-mode sensor for environmental water monitoring applications.
摘要：
Water purification technology that can simultaneously remove uranium, degrade organic pollutants, and inactivate pathogenic bacteria is crucial for protecting human health and the environment. In this study, we developed a self-driven photoelectrocatalytic (PEC) system using a sulfamic acid-modified carbon felt (CF/SA) cathode and a TiO 2 nanorod photoanode, both of which were obtained by hydrothermal methods. The system demonstrated excellent water purification performance in complex aqueous environments under sunlight illumination. The CF/SA cathode enabled improved interfacial charge transfer and more surface binding sites for uranium (UO 2 2+ ) adsorption and reduction. In the presence of both UO 2 2+ and sulfamethoxazole, almost 100 % UO 2 2+ was removed within 30 min, and 98.98 % sulfamethoxazole was degraded within 1.5 h. The self-driven PEC system also demonstrated effective antibacterial properties by completely inactivating E. coli within 25 min. Variations in wastewater characteristics, such as ions, pH, and organic concentrations had minimal impacts on the stability of the system. It also functioned well under real sunlight and exhibited excellent stability in long-term operation. This work demonstrated an effective cathode material to enhance the uranium reduction and antibiotics decomposition in a self-driven PEC system and provided a new solution for inactivating aqueous pathogens using sunlight only.
Water purification technology that can simultaneously remove uranium, degrade organic pollutants, and inactivate pathogenic bacteria is crucial for protecting human health and the environment. In this study, we developed a self-driven photoelectrocatalytic (PEC) system using a sulfamic acid-modified carbon felt (CF/SA) cathode and a TiO 2 nanorod photoanode, both of which were obtained by hydrothermal methods. The system demonstrated excellent water purification performance in complex aqueous environments under sunlight illumination. The CF/SA cathode enabled improved interfacial charge transfer and more surface binding sites for uranium (UO 2 2+ ) adsorption and reduction. In the presence of both UO 2 2+ and sulfamethoxazole, almost 100 % UO 2 2+ was removed within 30 min, and 98.98 % sulfamethoxazole was degraded within 1.5 h. The self-driven PEC system also demonstrated effective antibacterial properties by completely inactivating E. coli within 25 min. Variations in wastewater characteristics, such as ions, pH, and organic concentrations had minimal impacts on the stability of the system. It also functioned well under real sunlight and exhibited excellent stability in long-term operation. This work demonstrated an effective cathode material to enhance the uranium reduction and antibiotics decomposition in a self-driven PEC system and provided a new solution for inactivating aqueous pathogens using sunlight only.
期刊：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES,2025年88(8):339-348 ISSN：1528-7394
通讯作者：
Liu, Jun;Zhan, CH
作者机构：
[Liu, Jun; Zhan, Chunhua; Yang, Yue; Yang, Fei; Wang, Yaqi] Univ South China, Sch Basic Med Sci, Sch Publ Hlth, Hunan Key Lab Typ Environm Pollut & Hlth Hazards,H, Hengyang 421001, Hunan, Peoples R China.;[Zeng, Wen; Zhan, Chunhua] Cent Hosp Shaoyang, Dept Publ Hlth, Shaoyang, Peoples R China.;[Yuan, Mei] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Neurol, Hengyang, Hunan, Peoples R China.;[Zhan, Chunhua] Univ South China, Key Lab Rare Pediat Dis, Minist Educ, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liu, J; Zhan, CH ] U;Univ South China, Sch Basic Med Sci, Sch Publ Hlth, Hunan Key Lab Typ Environm Pollut & Hlth Hazards,H, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Key Lab Rare Pediat Dis, Minist Educ, Hengyang 421001, Hunan, Peoples R China.
关键词：
MC-LR;Apoptosis;TNF-R1;RIPK1;HEK293
摘要：
In recent years, the outbreak of cyanobacterial blooms has become increasingly frequent. Microcystin-LR (MC-LR), a metabolite of cyanobacteria, poses a significant threat to the ecosystem and human health. Several studies have demonstrated that MC-LR might induce renal cell apoptosis, as a consequence of tissue damage. However, the molecular mechanisms underlying MC-LR-initiated renal injury remain to be determined. This investigation aimed to determine the role of apoptosis in MC-LR-induced kidney damage and its potential underlying mechanisms using the human embryonic kidney (HEK293) cell line. The results of TUNEL and immunofluorescence assays indicated that MC-LR induced increased apoptosis in HEK293 cells. Compared to control, the mRNA expression levels of RIPK1, caspase-8, and TNF-α were elevated following incubation with MC-LR, while the mRNA expression level of Bcl-2/Bax was decreased. The protein levels of RIPK1, TNF-R1, and caspase-8 were elevated in the MC-LR-treated HEK293 cells. Data demonstrated that MC-LR induced renal cell apoptosis through activation of the TNF-R1/RIPK1 pathway, providing new insights into understanding the toxic mechanisms attributed to MC-LR.
期刊：
Food Safety and Health,2025年3(1):89-99 ISSN：2835-1096
通讯作者：
Xiangheng Niu
作者机构：
[Ziyu Zhang; Lina Tang; Wenjie Sheng; Youyi Yang; Guolin Lai; Jinjin Liu] School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, China;[Xiangheng Niu] School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, China
通讯机构：
[Xiangheng Niu] S;School of Public Health, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao, China
摘要：
AbstractAs a common food additive, excessive nitrite poses a great threat to human health, and monitoring its content in food products is of importance for healthy diet. Currently, the detection of nitrite content in food is primarily focused on fresh dishes, and there is a lack of research on monitoring the variations of nitrite in different foods under various storage conditions. In this study, we cascaded nanozyme catalysis with diazotization reaction and developed a ratiometric colorimetric assay to dynamically analyze nitrite in leftovers. First, nanoscale MnFe2O4 was synthesized as an oxidase mimic to catalyze colorless 3,3′,5,5′‐tetramethylbenzidine (TMB) oxidation to blue TMBox. Then, a diazotization process of the produced TMBox took place under the stimulation of nitrite, lowering the ultraviolet‐visible absorption signal (652 nm) assigned to TMBox and simultaneously generating a new signal at 445 nm ascribed to diazotized TMBox. Thus, a ratiometric colorimetric method could be constructed based on the above reversed variations of the two signals for high‐selectivity nitrite determination, providing a linear range of 1.76–180 μM and a detection limit of 0.12 μM. By employing the established assay to dynamically monitor nitrite in food products, it was found that the nitrite content in overnight leftovers was higher than that in fresh dishes, presenting an increasing trend with storage time. In addition, potential impacts of storage factors on the dynamics of nitrite content were investigated, providing some implications for food preservation and daily consumption.
期刊：
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH,2025年51(1):e16120- ISSN：1341-8076
通讯作者：
Wu, CQ
作者机构：
[Wu, CQ; Xie, Yinghao; Wu, Chengqiu; Zhu, Qiong] Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.;[Liu, Jun] Hengyang Cent Dis Control & Prevent, Hengyang, Peoples R China.
通讯机构：
[Wu, CQ ] U;Univ South China, Sch Publ Hlth, Hengyang, Peoples R China.
关键词：
CD1a;ovarian cancer;prognosis;recurrence;recurrent ovarian cancer
摘要：
OBJECTIVE: Explored the correlation between CD1a expression in recurrence and prognosis of ovarian cancer (OV). METHODS: The CD1a expression profile in OV, recurrent OV, and normal tissues, as well as corresponding clinical data, were obtained from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC), Gene Expression Omnibus (GEO), and Genotype Tissue Expression (GTEx) databases. Meanwhile, immunohistochemical detection of CD1a expression in normal and OV tissues. Kaplan-Meier curves were plotted to estimate the hazard ratio (HR) of survival in OV. In addition, the correlation between CD1a and immune cells in OV, as well as the CD1a expression profile and corresponding survival time in pan-cancer were obtained from TCGA database. RESULTS: CD1a was overexpressed in OV and was significantly under-expressed in recurrent OV (TCGA-OV, p < 0.0001 and ICGC-OV, p < 0.0001). CD1a immunohistochemistry is significantly overexpressed in OV compared to normal tissue (p < 0.05). Recurrent OV (ICGC, p < 0.001; GSE17260, p < 0.001; GSE32062, p < 0.05). The prognosis in OV was significantly better when CD1a is overexpressed compared to under-expressed (HR [low], 1.426: 95% confidence interval [CI], 0.912-2.128; p = 0.050). Meanwhile, the overexpression of CD1a has a better prognosis than low expression in OV and recurrent OV (p = 0.004, HR [low] = 2.462, 95%CI [1.346-4.504] and p = 0.011, HR [low] = 2.199, 95%CI [1.202-4.024]). In addition, CD1a expression was closely correlated with immune cells, the CD8+ T cells, macrophages, and NK cells, while uncharacterized cells were significantly different (p = 2.65e-6, p = 7.52e-13, p = 8.28e-12, and p = 5.89e-8, respectively). Moreover, CD1a expression affected the prognosis in various other cancers. CONCLUSIONS: CD1a expression affected the recurrence and prognosis of OV and is closely related to various immune cell levels.
期刊：
WATER AIR AND SOIL POLLUTION,2025年236(1):1-12 ISSN：0049-6979
通讯作者：
Xue, JH
作者机构：
[Wang, Xinqing; Huang, Boshi; Xue, Jinhua; Xiao, Xilin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[Lin, Dongying] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jingjing; Ren, Lanxing] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xiao, Xilin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Hunan, Peoples R China.
通讯机构：
[Xue, JH ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.
关键词：
Cadmium (II);FR;PAN;Fluorescence quenching
摘要：
The objective of this study was to establish a novel method of fluorescence for the determination of cadmium using fluorescein (FR) and 1-(2-Pyridinylazo)-2-Naohthalenol (PAN). In the pH 9.75 buffer solution of the ammonia-ammonium chloride, the energy transfer between FR and the PAN can occur, which can result in the quenching of the fluorescence. The addition of Cd (II) to this system enhances the degree of quenching. This phenomenon has led to the establishment of a novel method for the determination of cadmium, which has been optimized in terms of the required experimental conditions. In the optimal experimental conditions, the fluorescence intensity of the system decreased linearly in the range of 7.81 x 10-7 to 2.14 x 10-5 mol L-1. The linear regression equation used in this study was F = 123.63 + 170.65c (x 10-6 mol L-1) under the correlation coefficient of 0.9951, the detection limit of 2.34 x 10-7 mol L-1, and the relative standard deviation of 0.18%. The proposed method was simple, sensitive, fast, and economical. The method can be employed for the determination of cadmium in real samples (tap-water, pond water and Xiangjiang River) with the results demonstrating consistency with the graphite furnace atomic absorption method (t-test). This approach offers an innovative method for the quantification of Cd (II).
作者机构：
[Dingxin Long] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[Fei Chen; Jing Cao] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China;[Bei Yu] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
通讯机构：
[Fei Chen] N;National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
关键词：
Carbon-14;Committed dose;14 C urea breath test;Occupational health
摘要：
Objective To monitor the radioactivity concentration of 14 C in the air of rooms used to perform 14 C urea breath tests, and evaluate the contamination status of 14 C released during such tests, and assess the occupational health risk to medical staff working in such areas.
To monitor the radioactivity concentration of 14 C in the air of rooms used to perform 14 C urea breath tests, and evaluate the contamination status of 14 C released during such tests, and assess the occupational health risk to medical staff working in such areas.
Methods 14 CO 2 in air was absorbed from the air and turned into calcium carbonate through a chemical reaction. Then, calcium carbonate was prepared into a suspension in a low-potassium glass vial. The sample was analyzed using a low-background liquid scintillation counter.
14 CO 2 in air was absorbed from the air and turned into calcium carbonate through a chemical reaction. Then, calcium carbonate was prepared into a suspension in a low-potassium glass vial. The sample was analyzed using a low-background liquid scintillation counter.
Results The radioactivity concentrations of 14 C in air of the breath-test rooms in hospitals and physical examination institutions range from 1.35 to 18.41 Bq/m 3 . The annual committed dose for medical staff was estimated to be between 2.01 × 10 −2 and 2.74 × 10 −1 μSv.
The radioactivity concentrations of 14 C in air of the breath-test rooms in hospitals and physical examination institutions range from 1.35 to 18.41 Bq/m 3 . The annual committed dose for medical staff was estimated to be between 2.01 × 10 −2 and 2.74 × 10 −1 μSv.
Conclusions Our results reveal a significant increase of 14 C radioactivity concentration in the air of breath-test rooms, but the resulting committed dose is much lower than the limits prescribed by the International Commission on Radiological Protection (ICRP). However, the potential risk of long-term exposure to low-dose radiation should be paid more attention.
Our results reveal a significant increase of 14 C radioactivity concentration in the air of breath-test rooms, but the resulting committed dose is much lower than the limits prescribed by the International Commission on Radiological Protection (ICRP). However, the potential risk of long-term exposure to low-dose radiation should be paid more attention.
摘要：
Cyanobacteria are photosynthetic prokaryotes and major contributors to global biogeochemical cycles. They possess powerful biological activity, high adaptability to extreme environment, rapid growth rate and genetic editability. Here, we review cyanobacteria with focus on their application in environmental pollution and biomedicine. Cyanobacteria can be used for pollutant removal, biofuel and biochar production, template for drug discovery, hypoxia-related disease therapy, anticancer, antiviral, antibacterial, antioxidant and antifibrotic. Spiral-shaped cyanobacteria are excellent carriers for drug delivery. The oxygen-producing capacity of cyanobacteria is promising in the treatment of hypoxia-related diseases.
作者机构：
[Liu, Jinquan; Xiao, Xilin; Wu, Qian; Xiao, XL; Liu, Zhen] Univ South China, Sch Publ Hlth, Hengyang Sch Med, Hengyang, Hunan, Peoples R China.;[Liu, Xing] Univ South China, Sch Nucl Sci & Technol, Hengyang, Hunan, Peoples R China.;[Xiao, Xilin; Xiao, XL] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Hunan, Peoples R China.
通讯机构：
[Xiao, XL ] U;Univ South China, Sch Publ Hlth, Hengyang Sch Med, Hengyang, Hunan, Peoples R China.;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha, Hunan, Peoples R China.
摘要：
Food is essential for the proper functioning of the human body, and small molecule contaminants, such as antibiotics, have become a growing concern due to their harmful effects on both biological systems and the environment. These contaminants can enter the food supply through the use of antibiotics in animals, potentially causing significant health and ecological damage. As a result, detecting these pollutants, especially at trace levels, has become increasingly important. Aptamer sensors have gained popularity for this purpose because of their high stability, specificity, ease of modification, and low cost. To improve the sensitivity of these sensors, various signal enhancement strategies are used. These strategies aim to better detect small molecule contaminants, with many relying on nanomaterials and nucleic acid amplification techniques to amplify signals. Nanomaterials, which come in different forms such as zero-dimensional, one-dimensional, two-dimensional, and three-dimensional, play a crucial role in improving the performance of these sensors. This article provides an overview of these signal enhancement approaches, discussing the challenges and potential future directions for the development of aptamers in food contamination detection.
期刊：
Chemical Communications,2025年61(18):3756-3759 ISSN：1359-7345
通讯作者：
Pan, Jiafeng;Yang, F
作者机构：
[Wang, Yuyan; Yang, F; Pan, Jiafeng; Zeng, Ying; Ren, Xiaoya; Yang, Fei] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;[Qiu, Jun] Hunan Childrens Hosp, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Yang, F ; Pan, JF] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
摘要：
Based on a Y-shaped wheel-mediated triple walker, an enzyme-free biosensor was reported for UO22+ detection. Due to the DNAzyme-driven mechanism, our walker was activated and produced a fluorescence signal for UO22+ assay. The sensor demonstrated ultra-sensitivity, good specificity and excellent accuracy, holding great promise for UO22+ sensing in complex water samples.
通讯机构：
[Yang, F ] U;[Chen, JH ] G;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.;Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Peoples R China.
摘要：
A sensitive fluorescence biosensor was developed for microcystin-LR (MC-LR) detection using H1, H2, and H3 DNA probes as sensing elements. The aptamer in H1 can recognize the target. H2 was labeled with FAM and BHQ. The MC-LR and H1 binding will activate the H2 and H3 self-assemblies through toehold-mediated strand displacement. In the formed products (MC-LR/H1/nH2/nH3), FAM and BHQ will be separated and a high FAM fluorescence signal can be observed for the MC-LR assay. The biosensor is sensitive with a detection limit of 53 fM. We further constructed several logic circuits (AND-AND cascaded circuit, feedforward circuit, and resource allocation circuit) using MC-LR, MC-LA, and MC-YR as the three inputs. The numbers 0 and 1 are used to code the input and output signals. The AND-AND cascade circuit can produce a high output signal only in the (111) input combination. In the feedforward circuit, MC-LR and MC-LA can activate the logic circuit to produce high signals, and MC-YR will inhibit the self-assembly and execute the negative feedforward operation. Through the rational design of the DNA probe hybridizations on four different magnetic beads (MBs), the resource allocation circuit can achieve an intelligent allocation of input information. Our proposed fluorescence biosensor can not only provide a sensitive platform for microcystin detection but also serve as a smart and intelligent logic system for microcystin sensing.
