作者机构：
[Dingxin Long] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China;[Fei Chen; Jing Cao] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China;[Bei Yu] School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
通讯机构：
[Fei Chen] N;National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China
关键词：
Carbon-14;Committed dose;14 C urea breath test;Occupational health
摘要：
Objective To monitor the radioactivity concentration of 14 C in the air of rooms used to perform 14 C urea breath tests, and evaluate the contamination status of 14 C released during such tests, and assess the occupational health risk to medical staff working in such areas.
To monitor the radioactivity concentration of 14 C in the air of rooms used to perform 14 C urea breath tests, and evaluate the contamination status of 14 C released during such tests, and assess the occupational health risk to medical staff working in such areas.
Methods 14 CO 2 in air was absorbed from the air and turned into calcium carbonate through a chemical reaction. Then, calcium carbonate was prepared into a suspension in a low-potassium glass vial. The sample was analyzed using a low-background liquid scintillation counter.
14 CO 2 in air was absorbed from the air and turned into calcium carbonate through a chemical reaction. Then, calcium carbonate was prepared into a suspension in a low-potassium glass vial. The sample was analyzed using a low-background liquid scintillation counter.
Results The radioactivity concentrations of 14 C in air of the breath-test rooms in hospitals and physical examination institutions range from 1.35 to 18.41 Bq/m 3 . The annual committed dose for medical staff was estimated to be between 2.01 × 10 −2 and 2.74 × 10 −1 μSv.
The radioactivity concentrations of 14 C in air of the breath-test rooms in hospitals and physical examination institutions range from 1.35 to 18.41 Bq/m 3 . The annual committed dose for medical staff was estimated to be between 2.01 × 10 −2 and 2.74 × 10 −1 μSv.
Conclusions Our results reveal a significant increase of 14 C radioactivity concentration in the air of breath-test rooms, but the resulting committed dose is much lower than the limits prescribed by the International Commission on Radiological Protection (ICRP). However, the potential risk of long-term exposure to low-dose radiation should be paid more attention.
Our results reveal a significant increase of 14 C radioactivity concentration in the air of breath-test rooms, but the resulting committed dose is much lower than the limits prescribed by the International Commission on Radiological Protection (ICRP). However, the potential risk of long-term exposure to low-dose radiation should be paid more attention.
期刊：
Separation and Purification Technology,2025年354:129241 ISSN：1383-5866
通讯作者：
Zhongran Dai
作者机构：
[Dai, Zhongran; Liang, Beichao; Chen, Lijie] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China;[Zhang, Weilin] College of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China;[Gao, Yuan] School of Chemical Engineering and Technology, China University of Mining & Technology, Xuzhou, Jiangsu 221116, China;[Li, Le] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China<&wdkj&>College of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China
通讯机构：
[Zhongran Dai] K;Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China
摘要：
Photocatalysis holds promise for extracting uranium from aqueous solution. Nevertheless, conventional approaches generally rely on sacrificial agents and anaerobic conditions to maintain photocatalytic efficiency, which increases costs and causes secondary pollution. Herein, we introduce the design and synthesis of an S-scheme ZnIn2S4/g-C3N4 (ZISCN) heterojunction photocatalyst for the efficient removal of uranium via in-situ generating ZnIn2S4 on g-C3N4. Photoelectric characterization and theoretical calculation indicate that ZISCN boosts the absorption of visible light and promotes the effective separation and migration of charge carriers by forming an internal electric field (IEF) at the S-scheme heterojunction interface. This configuration integrates the strong reducing electrons of g-C3N4 and the potent oxidation holes of ZnIn2S4. Consequently, the as-synthesized ZISCN can efficiently remove uranium under an air atmosphere without the need for sacrificial agents and anaerobic conditions. The achieved U(VI) removal rate of 94.8 % surpasses that of ZnIn2S4 and g-C3N4 individually. Moreover, the photocatalytic extraction of U(VI) by ZISCN photocatalyst demonstrated excellent stability and anti-interference performance. After five cycles, the U(VI) removal rate remained above 85 %. Mechanism studies reveal that when electrons are generated by light in the ZISCN systems, they can reduce O2, leading to the formation of reactive species ·O2/H2O2. These species subsequently interact with U(VI), resulting in the precipitation of (UO2)O2·2H2O on the surface of ZISCN. This research provides valuable insights for the design of heterojunction photocatalysts for efficient, sacrificial agent-free uranium removal in ambient air environments.
摘要：
Inflammatory bowel disease (IBD) is a complex gastrointestinal disorder attributed to genetic and environmental factors. Microcystin-leucine-arginine (MC-LR) is an environmental toxin that accumulates in the gut and produces intestinal damage. The aim of this study was to investigate the effects of exposure to MC-LR on development and progression of IBD as well examine the underlying mechanisms of microcystin-initiated tissue damage. Male C57BL/6 mice were treated with either MC-LR alone or concurrently with dextran-sulfate sodium (DSS). Mice were divided into 4 groups (1): PBS gavage (control, CT) (2); 200μg/kg MC-LR gavage (MC-LR) (3); 3% DSS Drinking Water (DSS); and (4) 3% DSS Drinking Water + 200μg/kg MC-LR gavage (DSS + MC-LR). The mice in each experimental group exhibited reduced body weight, shortened colon length, increased disease activity index (DAI) score, a disrupted intestinal barrier, and elevated levels of proinflammatory cytokines compared to control. Compared to the group treated with MC-LR alone, colitis symptoms were exacerbated following combined exposure to both DSS and MC-LR. Subsequent experiments confirmed that MC-LR or DSS increased protein phosphorylation levels of Janus Kinase1 (JAK1) and Signal Transducer and Activator of Transcription3 (STAT3). Compared to group treated with MC-LR alone, the combined treatment of DSS and MC-LR also significantly upregulated the expression of related proteins. In conclusion, our study indicates that MC-LR-induced colitis involves activation of JAK1/STAT3 signaling pathway and that MC-LR exacerbates DSS-induced colitis through the same pathway.
摘要：
Microcystin-LR (MC-LR), produced by cyanobacterial harmful algal blooms, poses a serious threat to aquatic ecosystems and human health. Biodegradation is an important method for MC-LR elimination. Aerobic biodegradation has been extensively studied and many bacteria were identified. However, few MC-degrading bacteria have been isolated from anaerobic environments, and these degradation mechanisms are poorly understood. The aim of this study was to collect anaerobic MC-degrading bacteria from lake sediments in Lake Taihu using acclimation culture to assess biodegradation. Five strains with MC-LR degradation ability were isolated, with strain A4 belonging to Citrobacter farmeri (C. farmeri). exhibiting the highest efficiency at 0.486 μg/ml/d. High-performance liquid chromatography (HPLC) identified two novel MC-LR degradation products. Further polymerase chain reaction (PCR) analysis suggested that C. farmeri A4 did not possess the known MC-degrading gene mlrABCD, suggesting the involvement of an mlrABCD-independent anaerobic degradation pathway. Data demonstrated that the bacterial strain C. farmeri A4 found in Lake Taihu exhibited high anaerobic MC-LR degradation properties, which indicated that anaerobic biodegradation may constitute an important biological method for MC-LR removal in natural environment.
作者机构：
[Tang, Ya] School of Public Health, Hengyang Medical College, University of South China, 28 Western Changsheng Road, Hengyang, 421001, Hunan, China;[Tang, Ya] Department of Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China;[Hao, Bo; Hu, Haihong; Zhan, Wendi; Zhu, Hongxia] Department of Pharmacy, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China;[Chen, Siyuan] Department of Breast and Thyroid Surgery, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China;[Xu, Xuefeng] Department of Function, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China
摘要：
NAD(P)H dehydrogenase quinone 1 (NQO1) is overexpressed in various cancers and is strongly associated with an immunosuppressive microenvironment and poor prognosis. In this study, we explored the role of NQO1 in the microenvironment, prognosis and immunotherapy of Hepatocellular carcinoma (HCC) using multi-omics analysis and machine learning. The results revealed that NQO1 was significantly overexpressed in HCC cells. NQO1(+)HCC cells were correlated with poor prognosis and facilitated tumor-associated macrophages (TAMs) polarization to M2 macrophages. We identified core NQO1-related genes (NRGs) and developed the NRGs-related risk-scores in hepatocellular carcinoma (NRSHC). The comprehensive nomogram integrating NRSHC, age, and pathological tumor-node-metastasis (pTNM) Stage achieved an area under the curve (AUC) above 0.7, demonstrating its accuracy in predicting survival outcomes and immunotherapy responses of HCC patients. High-risk patients exhibited worse prognoses but greater sensitivity to immunotherapy. Additionally, a web-based prediction tool was designed to enhance clinical utility. In conclusion, NQO1 may play a critical role in M2 polarization and accelerates HCC progression. The NRSHC model and accompanying tools offer valuable insights for personalized HCC treatment.
摘要：
Radiation therapy is an important method to treat liver cancer, but because of the strong DNA repair ability of liver cancer cells, even after receiving high doses of radiation still can not get satisfactory results. Atorvastatin (ATO) is a lipophilic and tissue-selective inhibitor of HMG-CoA reductase whose anticancer effects have been validated in various cells, but its effect on the radiation sensitivity of hepatocellular carcinoma cells remains unclear. Therefore, Therefore, this study explored the radiosensitivity of ATO and its possible mechanism by pretreating HepG2 with ATO and collecting HepG2 cells after irradiation. It was found that atorvastatin can not only affect the survival of liver cancer cells when used alone, but also enhance the radiation sensitivity of HepG2 cells. The study found that ATO significantly exacerbated the inhibitory effects of IR on the growth, proliferation, and migration of HepG2 cells. Measurement of ROS, SOD, GPx, and MDA levels indicated that ATO enhanced IR-induced oxidative stress, further promoted the decrease of Mitochondrial Membrane Potential, increased the rate of apoptosis in HepG2, upregulating pro-apoptotic proteins Bax and Cleaved-Caspase 3, and downregulating anti-apoptotic proteins Bcl-2. Western blot analysis showed that the PI3K-Akt-mTOR pathway was inhibited, leading to the activation of cytotoxic autophagy in HepG2 and an increase in the expression of the LC-3II protein. In summary, ATO, in combination with IR, enhances the oxidative stress response of HepG2 induced by IR, promotes autophagy by inhibiting the PI3K-Akt-mTOR pathway, and thereby potentially enhances the radiosensitivity of HepG2 as a pharmacological intervention.
期刊：
Sensors and Actuators B-Chemical,2025年433:137564 ISSN：0925-4005
通讯作者：
Le Li
作者机构：
[Yao Yu; Xian Tang; Le Li] Cancer Research Institute, The First Affiliated Hospital, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China;[Qingyun Cai] State Key Laboratory of Chemo/Biosensing and Chemometrics, School of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China;[Craig A. Grimes] Flux Photon Corporation, 5950 Shiloh Road East, Alpharetta, GA 30005, United States;[Yu Liu; Chunlin Liu; Qiuhui Deng; Shengyuan Yang; Deshuai Zhen] Cancer Research Institute, The First Affiliated Hospital, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, School of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China
通讯机构：
[Le Li] C;Cancer Research Institute, The First Affiliated Hospital, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, PR China
摘要：
Nuclear accidents, uranium mining, and nuclear weapons production pose significant health and environmental concerns, and as a consequence there is ongoing interest in techniques for rapid and selective detection of the uranyl ion (UO 2 2+ ). In this study, a β-keto-enamine-linked covalent organic framework (COF) fluorescent probe was synthesized by a simple one-step condensation reaction using 1,3,5-triformylphoroglucinol (Tp) and 3,3′-dihydroxybenzidine (Db). Tp-Db exhibited a UO 2 2+ detection limit of 99.34 nM and a 10 s reaction time, making it suitable for rapid detection in diverse environmental samples including river water, nuclear wastewater, food, and urine. Experimental analyses and density-functional theory (DFT) calculations reveal that UO 2 2+ preferentially coordinates with the carbonyl group in Tp-Db through intramolecular charge transfer and electrostatic interactions, and the presence of the hydroxyl assistant group further improves the binding affinity. This study elucidates the interaction mechanism of different functional groups (carbonyl, hydroxyl, and imine) with UO 2 2+ within the channels of the keto-enamine-linked COF, providing a promising rational basis for the development of an advanced UO 2 2+ sensing platform.
Nuclear accidents, uranium mining, and nuclear weapons production pose significant health and environmental concerns, and as a consequence there is ongoing interest in techniques for rapid and selective detection of the uranyl ion (UO 2 2+ ). In this study, a β-keto-enamine-linked covalent organic framework (COF) fluorescent probe was synthesized by a simple one-step condensation reaction using 1,3,5-triformylphoroglucinol (Tp) and 3,3′-dihydroxybenzidine (Db). Tp-Db exhibited a UO 2 2+ detection limit of 99.34 nM and a 10 s reaction time, making it suitable for rapid detection in diverse environmental samples including river water, nuclear wastewater, food, and urine. Experimental analyses and density-functional theory (DFT) calculations reveal that UO 2 2+ preferentially coordinates with the carbonyl group in Tp-Db through intramolecular charge transfer and electrostatic interactions, and the presence of the hydroxyl assistant group further improves the binding affinity. This study elucidates the interaction mechanism of different functional groups (carbonyl, hydroxyl, and imine) with UO 2 2+ within the channels of the keto-enamine-linked COF, providing a promising rational basis for the development of an advanced UO 2 2+ sensing platform.
期刊：
Process Safety and Environmental Protection,2025年:106982 ISSN：0957-5820
通讯作者：
Xiaoya Ren
作者机构：
[Yafang Li; Jia Wei; Sisi Yan; Chunhua Zhan; Xiaoya Ren] School of Public Health, University of South China, Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, Hengyang 421001, Hunan, China;[Xiaofang Liang] Clinical college of Anhui medical university, Hefei 230032, Anhui, China;[Jiangping Wang] Hunan Engineering Research Center of Livestock and Poultry Health Care, Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China;[Lemei Zhu] School of Public Health, Changsha Medical University, Changsha 410219, Hunan, China;[Zhen Ding] Department of Environment and Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, Jiangsu, China
通讯机构：
[Xiaoya Ren] S;School of Public Health, University of South China, Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, Hengyang 421001, Hunan, China
摘要：
Microcystin-LR (MC-LR) is a widely distributed cyanotoxin that causes kidney injury. Our previous work identified Sphingopyxis sp. YF1 as a novel bacterial strain capable of efficiently degrading MC-LR, but its protective role and mechanisms against MC-LR-induced kidney injury remain unexplored. In this study, the bioactive metabolite of strain YF1 was identified by mass spectrometry. The beneficial effect of strain YF1 and its bioactive metabolite against MC-LR-induced kidney injury was validated in vivo and in vitro . We found strain YF1 effectively ameliorated MC-LR induced kidney disruption and functional impairment. Mechanistically, strain YF1 significantly improved oxidative stress (SOD, CAT, GSH, and MDA) and inhibited pro-inflammatory factors expression (including IL-6 , IL-18 , IL-1β , and TNF-α ) induced by MC-LR. Notably, strain YF1 not only reduce MC-LR level in mice but also produce a bioactive metabolite astaxanthin. Consistently, both in vivo and in vitro experiments demonstrated that astaxanthin alleviated MC-LR-induced kidney injury by modulating antioxidant pathways and inflammatory cascades. This study suggested that strain YF1 could alleviate MC-LR-induced kidney injury by removing MC-LR and generating astaxanthin that mediated antioxidant and anti-inflammatory effects. These findings highlighted microbial-based antidotes for simultaneously eliminating MC-LR and mitigating its harmful effects.
Microcystin-LR (MC-LR) is a widely distributed cyanotoxin that causes kidney injury. Our previous work identified Sphingopyxis sp. YF1 as a novel bacterial strain capable of efficiently degrading MC-LR, but its protective role and mechanisms against MC-LR-induced kidney injury remain unexplored. In this study, the bioactive metabolite of strain YF1 was identified by mass spectrometry. The beneficial effect of strain YF1 and its bioactive metabolite against MC-LR-induced kidney injury was validated in vivo and in vitro . We found strain YF1 effectively ameliorated MC-LR induced kidney disruption and functional impairment. Mechanistically, strain YF1 significantly improved oxidative stress (SOD, CAT, GSH, and MDA) and inhibited pro-inflammatory factors expression (including IL-6 , IL-18 , IL-1β , and TNF-α ) induced by MC-LR. Notably, strain YF1 not only reduce MC-LR level in mice but also produce a bioactive metabolite astaxanthin. Consistently, both in vivo and in vitro experiments demonstrated that astaxanthin alleviated MC-LR-induced kidney injury by modulating antioxidant pathways and inflammatory cascades. This study suggested that strain YF1 could alleviate MC-LR-induced kidney injury by removing MC-LR and generating astaxanthin that mediated antioxidant and anti-inflammatory effects. These findings highlighted microbial-based antidotes for simultaneously eliminating MC-LR and mitigating its harmful effects.
摘要：
Rapid and accurate detection of Chlamydia psittaci , the causative agent of psittacosis, is crucial for both human and animal health but presents significant challenges, particularly in grassroots health institutions. Our previous PDTCTR fluorescence sensing platform, which combined the engineered Cas12f1_ge4.1 system with recombinase polymerase amplification (RPA), significantly enhanced detection efficiency. However, its requirement for specialized equipment, costly RPA reagents, and absence of visual output restricted its practical application in such environments. To address these limitations, we developed the ERA/Cas12f1_ge4.1 system, integrating Cas12f1_ge4.1 with the cost-effective Enzymatic Recombinase Amplification (ERA). This system enables sensitive detection of Chlamydia psittaci double-stranded DNA within 50 min through both fluorescence and colloidal gold lateral flow assay strips. The platform achieves detection limits of 10 copies/μL for fluorescence and 100 copies/μL for lateral flow. Clinical validation involving 93 parrot samples demonstrated high performance in both detection modes. Fluorescence detection achieved 95.4 % sensitivity, 100 % specificity, a 100 % positive predictive value (PPV), and a 90.3 % negative predictive value (NPV). Meanwhile, the lateral flow assay exhibited 92.3 % sensitivity, 100 % specificity, 100 % PPV, and an 84.8 % NPV. The ERA/Cas12f1_ge4.1 system offers a rapid, accurate, cost-effective, and visually interpretable diagnostic tool suitable for both laboratory and community health centers. This advancement holds significant potential for improving psittacosis diagnosis and control, particularly in resource-limited environments.
Rapid and accurate detection of Chlamydia psittaci , the causative agent of psittacosis, is crucial for both human and animal health but presents significant challenges, particularly in grassroots health institutions. Our previous PDTCTR fluorescence sensing platform, which combined the engineered Cas12f1_ge4.1 system with recombinase polymerase amplification (RPA), significantly enhanced detection efficiency. However, its requirement for specialized equipment, costly RPA reagents, and absence of visual output restricted its practical application in such environments. To address these limitations, we developed the ERA/Cas12f1_ge4.1 system, integrating Cas12f1_ge4.1 with the cost-effective Enzymatic Recombinase Amplification (ERA). This system enables sensitive detection of Chlamydia psittaci double-stranded DNA within 50 min through both fluorescence and colloidal gold lateral flow assay strips. The platform achieves detection limits of 10 copies/μL for fluorescence and 100 copies/μL for lateral flow. Clinical validation involving 93 parrot samples demonstrated high performance in both detection modes. Fluorescence detection achieved 95.4 % sensitivity, 100 % specificity, a 100 % positive predictive value (PPV), and a 90.3 % negative predictive value (NPV). Meanwhile, the lateral flow assay exhibited 92.3 % sensitivity, 100 % specificity, 100 % PPV, and an 84.8 % NPV. The ERA/Cas12f1_ge4.1 system offers a rapid, accurate, cost-effective, and visually interpretable diagnostic tool suitable for both laboratory and community health centers. This advancement holds significant potential for improving psittacosis diagnosis and control, particularly in resource-limited environments.
期刊：
Science of The Total Environment,2025年970:179016 ISSN：0048-9697
通讯作者：
Zhou, Bin;Yang, Fei;Zeng, Qiang
作者机构：
[Gui, Ping-Ping] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China;[Zeng, Jia-Yue; Liu, Xiao-Ying; Miao, Yu; Li, Cheng-Ru; Zhu, Jin-Qin; Zhang, Min; Liu, A-Xue; Deng, Yan-Ling; Wu, Yang; Liu, Peng-Hui; Li, Yang-Juan; Zeng, Qiang] Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;[Zeng, Jia-Yue; Liu, Xiao-Ying; Miao, Yu; Li, Cheng-Ru; Zhu, Jin-Qin; Zhang, Min; Liu, A-Xue; Deng, Yan-Ling; Wu, Yang; Liu, Peng-Hui; Li, Yang-Juan] Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China;[Deng, Yan-Ling] Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA;[Zhang, Min] Department of Environmental Medicine and Climate Science, Icahn School of Medicine at Mount Sinai, New York, NY, USA
通讯机构：
[Yang, Fei] H;[Zeng, Qiang] K;[Zhou, Bin] W;Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China. Electronic address:;Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China. Electronic address:
关键词：
Dichloroacetic acid;Disinfection byproducts;Liver function parameters;Trichloroacetic acid
摘要：
Background Toxicological studies have documented that disinfection byproducts (DBPs), the ubiquitous drinking water pollutants, induce hepatotoxicity. Yet epidemiological evidence is sparse.
Toxicological studies have documented that disinfection byproducts (DBPs), the ubiquitous drinking water pollutants, induce hepatotoxicity. Yet epidemiological evidence is sparse.
Objective To assess urinary biomarkers of drinking water DBPs in relation to liver function parameters.
To assess urinary biomarkers of drinking water DBPs in relation to liver function parameters.
Methods We included 1204 reproductive-aged women from the Tongji Reproductive and Environmental (TREE) study in Wuhan, China between December 2018 and July 2021. Urinary trichloroacetic acid (TCAA) and dichloroacetic acid (DCAA) as biomarkers of drinking water DBPs were assessed. Serum liver function parameters such as albumin (ALB), total cholesterol (TC), and alkaline phosphatase (ALP) were determined. Urinary DCAA and TCAA concentrations in relation to liver function parameters were examined by multivariate linear regression or restricted cubic spline (RCS) models.
We included 1204 reproductive-aged women from the Tongji Reproductive and Environmental (TREE) study in Wuhan, China between December 2018 and July 2021. Urinary trichloroacetic acid (TCAA) and dichloroacetic acid (DCAA) as biomarkers of drinking water DBPs were assessed. Serum liver function parameters such as albumin (ALB), total cholesterol (TC), and alkaline phosphatase (ALP) were determined. Urinary DCAA and TCAA concentrations in relation to liver function parameters were examined by multivariate linear regression or restricted cubic spline (RCS) models.
Results There was no evidence of urinary TCAA in relation to serum parameters of liver function. However, monotonic dose-response relationships were estimated between elevated tertiles of urinary DCAA concentrations and increased serum ALP (percent change = 4.25 %; 95 % CI: 0.34 %, 8.32 % for the upper vs. lower tertile) and TC levels (percent change = 3.84 %; 95 % CI: 0.63 %, 7.17 % for the upper vs. lower tertile). These associations remained for urinary DCAA modeled as the continuous exposure variable and were linear in the RCS models. Age, body mass index, and passive smoking status did not modify these associations.
There was no evidence of urinary TCAA in relation to serum parameters of liver function. However, monotonic dose-response relationships were estimated between elevated tertiles of urinary DCAA concentrations and increased serum ALP (percent change = 4.25 %; 95 % CI: 0.34 %, 8.32 % for the upper vs. lower tertile) and TC levels (percent change = 3.84 %; 95 % CI: 0.63 %, 7.17 % for the upper vs. lower tertile). These associations remained for urinary DCAA modeled as the continuous exposure variable and were linear in the RCS models. Age, body mass index, and passive smoking status did not modify these associations.
Conclusion DCAA but not TCAA exposure may contribute to damaged liver function in reproductive-aged women.
DCAA but not TCAA exposure may contribute to damaged liver function in reproductive-aged women.
摘要：
Rheumatoid arthritis (RA) is a common chronic autoimmune disease that primarily affects the joints, leading to synovial inflammation and hyperplasia, which subsequently causes joint pain, swelling, and damage. The microenvironment of RA is characterized by hypoxia, high reactive oxygen species (ROS), low pH, and levels of high inflammatory factors. Traditional treatments only partially alleviate symptoms and often cause various adverse reactions with long-term use. Therefore, there is an urgent need for safer and more effective treatments. In recent years, mesenchymal stem cells (MSCs) have shown significant potential in treating RA due to their diverse immunomodulatory mechanisms. MSCs paracrine a variety of soluble factors to improve the inflammatory microenvironment in RA patients by inhibiting T cell proliferation or inducing T cell differentiation to regulatory T cells (Tregs), inhibiting B cell proliferation and differentiation and immunoglobulin production, prompting macrophage polarization toward an anti-inflammatory phenotype, and inhibiting neutrophil recruitment and preventing the maturation of dendritic cells (DCs). This review summarizes the immunomodulatory effects of MSCs in RA and their application in animal models and clinical trials. Although the immunomodulatory mechanisms of MSCs are not yet fully elucidated, their significant potential in RA treatment has been widely recognized. Future research should further explore the immunomodulatory mechanisms of MSCs and optimize their functions in different pathological microenvironments to develop more effective and safer therapeutic strategies.
Rheumatoid arthritis (RA) is a common chronic autoimmune disease that primarily affects the joints, leading to synovial inflammation and hyperplasia, which subsequently causes joint pain, swelling, and damage. The microenvironment of RA is characterized by hypoxia, high reactive oxygen species (ROS), low pH, and levels of high inflammatory factors. Traditional treatments only partially alleviate symptoms and often cause various adverse reactions with long-term use. Therefore, there is an urgent need for safer and more effective treatments. In recent years, mesenchymal stem cells (MSCs) have shown significant potential in treating RA due to their diverse immunomodulatory mechanisms. MSCs paracrine a variety of soluble factors to improve the inflammatory microenvironment in RA patients by inhibiting T cell proliferation or inducing T cell differentiation to regulatory T cells (Tregs), inhibiting B cell proliferation and differentiation and immunoglobulin production, prompting macrophage polarization toward an anti-inflammatory phenotype, and inhibiting neutrophil recruitment and preventing the maturation of dendritic cells (DCs). This review summarizes the immunomodulatory effects of MSCs in RA and their application in animal models and clinical trials. Although the immunomodulatory mechanisms of MSCs are not yet fully elucidated, their significant potential in RA treatment has been widely recognized. Future research should further explore the immunomodulatory mechanisms of MSCs and optimize their functions in different pathological microenvironments to develop more effective and safer therapeutic strategies.
摘要：
Sensor arrays have been widely used in the identification of metal ions (MIs), whereas traditional sensor arrays often rely on multiple independent sensing elements, which requires higher cost, complicated synthesis, and time-consuming. Herein, we designed a nanozyme sensor array based on a single element with three colorimetric channels for the discrimination of seven MIs (Pb 2+ , Fe 3+ , Zr 4+ , Mg 2+ , Cr 3+ , Ag + , and Hg 2+ ) in environments. Wherein, the Pt/HCNs nanozyme was prepared by integrating hollow carbon nanospheres (HCNs) with platinum nanoparticles, which exhibited enhanced oxidase-like activity and could directly catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to produce three absorption peaks at 370, 450, and 650 nm, providing a single element with three colorimetric channels. Interestingly, due to the interaction of MIs and Pt/HCNs, the MIs differentially regulated the intensity of three absorption peaks of the Pt/HCNs-TMB system, generating distinct colorimetric “fingerprints” that can be further identified through pattern recognition methods. The proposed nanozyme sensor array achieved 100 % accuracy in identifying various MIs and multi-component mixtures, which also showed good performance in the discrimination of MIs in real environmental waters. Thus, our findings provided some new insights for construction of sensor arrays and improving the effective identification of MIs in environments.
Sensor arrays have been widely used in the identification of metal ions (MIs), whereas traditional sensor arrays often rely on multiple independent sensing elements, which requires higher cost, complicated synthesis, and time-consuming. Herein, we designed a nanozyme sensor array based on a single element with three colorimetric channels for the discrimination of seven MIs (Pb 2+ , Fe 3+ , Zr 4+ , Mg 2+ , Cr 3+ , Ag + , and Hg 2+ ) in environments. Wherein, the Pt/HCNs nanozyme was prepared by integrating hollow carbon nanospheres (HCNs) with platinum nanoparticles, which exhibited enhanced oxidase-like activity and could directly catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to produce three absorption peaks at 370, 450, and 650 nm, providing a single element with three colorimetric channels. Interestingly, due to the interaction of MIs and Pt/HCNs, the MIs differentially regulated the intensity of three absorption peaks of the Pt/HCNs-TMB system, generating distinct colorimetric “fingerprints” that can be further identified through pattern recognition methods. The proposed nanozyme sensor array achieved 100 % accuracy in identifying various MIs and multi-component mixtures, which also showed good performance in the discrimination of MIs in real environmental waters. Thus, our findings provided some new insights for construction of sensor arrays and improving the effective identification of MIs in environments.
作者机构：
[Lei Zhang; Kaimin Chen; Luchang Li; Xiu Wei; Xianxin Cai; Yaohui Yu; Yixian Yang; Renping Cao; Fenghua Wei; Bang Lan; Xiaohu Luo; Hui Liang] Northeast Guangdong Key Laboratory of New Functional Materials, Guangdong Rare Earth Photofunctional Materials Engineering Technology Research Center, Meizhou Rare Earth Photo functional Materials Engineering Technology Research Center, Office of Assets&Laboratory Management, School of Chemistry and Environment, Jiaying University, Meizhou, 514015, China;[Deshuai Zhen] Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Yanhua Li] School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang, 421001, China
通讯机构：
[Yanhua Li] S;[Hui Liang] N;[Deshuai Zhen] H;Northeast Guangdong Key Laboratory of New Functional Materials, Guangdong Rare Earth Photofunctional Materials Engineering Technology Research Center, Meizhou Rare Earth Photo functional Materials Engineering Technology Research Center, Office of Assets&Laboratory Management, School of Chemistry and Environment, Jiaying University, Meizhou, 514015, China<&wdkj&>School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang, 421001, China<&wdkj&>Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China
摘要：
Developing non-precious electrocatalysts with high-efficiency hydrogen evolution reaction (HER) activity for hydrogen production at all pH values is crucial for the application and promotion of water electrolysis. Herein, a unique structure of nickel-cobalt (Ni-Co) nanoparticles@Ni 0.19 Co 0.26 P nanowires core/shell arrays on Ni foam as a pH-universal electrocatalyst for hydrogen evolution is synthesized by a simple hydrothermal process and thermal reduction treatment. The morphological analysis reveals that numerous Ni-Co nanoparticles are densely packed inside the hollow interior of Ni 0.19 Co 0.26 P nanowires. This unique structure integrates the advantages of high surface area from the nanowires and enhanced electronic conductivity from the Ni-Co nanoparticles, effectively addressing the conventional issue of slow charge transfer rate in 3D metal-based compounds, which is typically caused by a long transfer distance. The electrocatalysts provide an excellent hydrogen evolution reaction performance in alkaline, neutral, and acidic media with an overpotential of 35, 36, and 23 mV to reach the current density of 10 mA/cm 2 , respectively. This study highlights the potential of Ni-Co nanoparticles@Ni 0.19 Co 0.26 P nanowires as a cost-effective and pH-universal HER electrocatalyst, offering promising prospects for sustainable hydrogen production.
Developing non-precious electrocatalysts with high-efficiency hydrogen evolution reaction (HER) activity for hydrogen production at all pH values is crucial for the application and promotion of water electrolysis. Herein, a unique structure of nickel-cobalt (Ni-Co) nanoparticles@Ni 0.19 Co 0.26 P nanowires core/shell arrays on Ni foam as a pH-universal electrocatalyst for hydrogen evolution is synthesized by a simple hydrothermal process and thermal reduction treatment. The morphological analysis reveals that numerous Ni-Co nanoparticles are densely packed inside the hollow interior of Ni 0.19 Co 0.26 P nanowires. This unique structure integrates the advantages of high surface area from the nanowires and enhanced electronic conductivity from the Ni-Co nanoparticles, effectively addressing the conventional issue of slow charge transfer rate in 3D metal-based compounds, which is typically caused by a long transfer distance. The electrocatalysts provide an excellent hydrogen evolution reaction performance in alkaline, neutral, and acidic media with an overpotential of 35, 36, and 23 mV to reach the current density of 10 mA/cm 2 , respectively. This study highlights the potential of Ni-Co nanoparticles@Ni 0.19 Co 0.26 P nanowires as a cost-effective and pH-universal HER electrocatalyst, offering promising prospects for sustainable hydrogen production.
作者机构：
[Liu, Yu; Li, Le; Liu, Jinquan; Tong, Yuqi; Zhen, Deshuai; Song, Jiayi; Xiong, Lihao; Chen, Sihan] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构：
[Li, L; Zhen, DS ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
关键词：
Fluorescence;Colorimetric detection;Calixarene;Rare-earth ions;Smartphone assisted color detection
摘要：
Anovel fluorescence/colorimetric dual-mode detection strategy for uranium ions (UO(2)(2+)) is presentedbased on a cerium-sulfonyl calix[4]arene (SC4A) platform. The exo- and endo-rim sites of SC4A can coordinate with Ce(3+) and Ce(4+) ions, respectively, quenching Ce(3+) fluorescence and influencing the oxidase-like activity of Ce(4+). In the absence of UO(2)(2+), the solution of 3,3,5,5-tetramethylbenzidine (TMB) remains blue, but upon UO(2)(2+) binding, Ce(3+) dissociates from SC4A, restoring fluorescence, while UO(2)(2+) interacts with oxTMB, turning the solution from blue to colorless. This dual-mode system provides a linear fluorescence detection range of 30-800nM with a detection limit of 20.20nM, and a colorimetric range of 30-800nM with a detection limit of 27.78nM. By combining high-sensitivity fluorescence with visual colorimetric analysis, the proposed method possesses high sensitivity, accuracy, and reliability. Notably, smartphone-based color capture facilitates rapid and convenient sample analysis, enabling straightforward quantification at varying UO(2)(2+) concentrations. The method has been successfully applied to real water and urine samples, demonstrating its practical utility in environmental and biological monitoring of UO(2)(2+).
摘要：
Microcystin LR (MC-LR) pollution is a serious threat to aquatic ecosystems and public health in China and is an environmental problem that urgently needs to be solved. However, few studies have investigated the anaerobic degradation pathway and related molecular biological mechanisms of MC-LR. In this study, a bacterium capable of degrading MC-LR with a degradation efficiency of 0.303 µg/mL/d under anaerobic conditions was isolated from water. The strain was identified as Alcaligenes and named Alcaligenes faecalis D04. Two new anaerobic degradation products, one pentapeptide (Adda-Glu-Mdha-Ala-Leu) and one tripeptide (Adda-Glu-Mdha), were identified by chromatography and mass spectrometry, and two new anaerobic degradation pathways for microcystins were proposed. This study revealed a new connection between related functional genes ( mblH , ridA , paaA , livI , soxR , gltD , marR , etc.) and bacterial degradation functions through the analysis of multiomics data. Real-time quantitative PCR analysis verified that the expression trends of the differentially expressed genes were consistent with the transcriptomic data. Our study aimed to elucidate the anaerobic degradation pathway and molecular regulatory mechanism of MC-LR in Alcaligenes faecalis D04, which offers important practical significance for microbial strategies to prevent and regulate microcystin contamination.
Microcystin LR (MC-LR) pollution is a serious threat to aquatic ecosystems and public health in China and is an environmental problem that urgently needs to be solved. However, few studies have investigated the anaerobic degradation pathway and related molecular biological mechanisms of MC-LR. In this study, a bacterium capable of degrading MC-LR with a degradation efficiency of 0.303 µg/mL/d under anaerobic conditions was isolated from water. The strain was identified as Alcaligenes and named Alcaligenes faecalis D04. Two new anaerobic degradation products, one pentapeptide (Adda-Glu-Mdha-Ala-Leu) and one tripeptide (Adda-Glu-Mdha), were identified by chromatography and mass spectrometry, and two new anaerobic degradation pathways for microcystins were proposed. This study revealed a new connection between related functional genes ( mblH , ridA , paaA , livI , soxR , gltD , marR , etc.) and bacterial degradation functions through the analysis of multiomics data. Real-time quantitative PCR analysis verified that the expression trends of the differentially expressed genes were consistent with the transcriptomic data. Our study aimed to elucidate the anaerobic degradation pathway and molecular regulatory mechanism of MC-LR in Alcaligenes faecalis D04, which offers important practical significance for microbial strategies to prevent and regulate microcystin contamination.
摘要：
In this research, a new synthesis approach was developed for an adsorbent, namely the phosphorylated ZIF-8/bamboo charcoal/chitosan/tannic acid (P-ZBCT) composite, for the efficient adsorption of uranyl ions from wastewater at low dosages. Impressively, the uranium adsorption rate of P-ZBCT reaches up to 98 % at a low dosage of 0.056 g/L in a 10-mg/L uranium solution, outperforming most reported uranium adsorption materials. The theoretical maximum adsorption capacity of P-ZBCT for uranium at 308 K and pH 6.0 is 2357.69 mg/g, with uranium adsorption being a spontaneous endothermic chemical reaction. Mechanistic analysis reveals that surface functional groups such as P O, amino group, and C N play a pivotal role in uranium adsorption. A competitive adsorption experiment shows that zinc is the most competitive with uranium adsorption; however, the partition coefficient of U is 11 times that of zinc, indicating that the absorption of uranium is more selective than that of other metal ions, such as zinc. Adsorption treatment using P-ZBCT successfully reduces the uranium content in real uranium tailings–containing pond wastewater to 34 μg/L. P-ZBCT demonstrates exceptional recycling performance, maintaining an adsorption rate of 85 % even after 10 sorption–desorption cycles. Therefore, P-ZBCT exhibits significant potential for efficiently extracting uranium from low-concentration uranium-containing wastewater.
In this research, a new synthesis approach was developed for an adsorbent, namely the phosphorylated ZIF-8/bamboo charcoal/chitosan/tannic acid (P-ZBCT) composite, for the efficient adsorption of uranyl ions from wastewater at low dosages. Impressively, the uranium adsorption rate of P-ZBCT reaches up to 98 % at a low dosage of 0.056 g/L in a 10-mg/L uranium solution, outperforming most reported uranium adsorption materials. The theoretical maximum adsorption capacity of P-ZBCT for uranium at 308 K and pH 6.0 is 2357.69 mg/g, with uranium adsorption being a spontaneous endothermic chemical reaction. Mechanistic analysis reveals that surface functional groups such as P O, amino group, and C N play a pivotal role in uranium adsorption. A competitive adsorption experiment shows that zinc is the most competitive with uranium adsorption; however, the partition coefficient of U is 11 times that of zinc, indicating that the absorption of uranium is more selective than that of other metal ions, such as zinc. Adsorption treatment using P-ZBCT successfully reduces the uranium content in real uranium tailings–containing pond wastewater to 34 μg/L. P-ZBCT demonstrates exceptional recycling performance, maintaining an adsorption rate of 85 % even after 10 sorption–desorption cycles. Therefore, P-ZBCT exhibits significant potential for efficiently extracting uranium from low-concentration uranium-containing wastewater.
期刊：
Journal of Cleaner Production,2025年492:144883 ISSN：0959-6526
通讯作者：
Jiajie Zhang
作者机构：
[Chuqiao Wang; Chaowei Kang; Shuiming Liu; Shan Huang; Yuying Hu; Jiajie Zhang] School of Civil Engineering and Architecture, East China Jiaotong University, Nanchang, 330013, China;[Xizi Long] Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Shuai Zhang] Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEE'T), Nanjing University of Information Science & Technology, Naning, 210044, China
通讯机构：
[Jiajie Zhang] S;School of Civil Engineering and Architecture, East China Jiaotong University, Nanchang, 330013, China
摘要：
Suppressing temperature stratification is crucial for pilot-scale methanogenic reactor (MR) in two-phase anaerobic digestion. In this study, computational fluid dynamics was utilized to simulate changes in temperature distribution under various heating and hydraulic stirring conditions within the MR. Elevating heating temperature accelerated the process of the MR reaching the set temperature (35.5 °C). However, excessively high heating temperature led to significant temperature differences. The maximum temperature difference varied from 2.15 °C to 4 °C when heating temperature ranged from 45 °C to 75 °C, respectively. Hydraulic stirring effectively mitigated temperature distribution, reducing the maximum temperature difference from 3 °C to 1 °C at stirring speed of 0.31 m/s when heating temperature was maintained at 55 °C. The standard deviation of the temperatures at points P1-P5 and the average temperature decreased from 0.22 at 0.11 m/s to 0.076 at 0.31 m/s. Consequently, cumulative biogas production of the MR increased from 8.085 m³ to 12.975 m³ after implementing hydraulic stirring. Microbial community analysis revealed that methanogens were more susceptible to the effects of temperature distribution compared to bacteria. This study provides guidance for practical project implementations.
Suppressing temperature stratification is crucial for pilot-scale methanogenic reactor (MR) in two-phase anaerobic digestion. In this study, computational fluid dynamics was utilized to simulate changes in temperature distribution under various heating and hydraulic stirring conditions within the MR. Elevating heating temperature accelerated the process of the MR reaching the set temperature (35.5 °C). However, excessively high heating temperature led to significant temperature differences. The maximum temperature difference varied from 2.15 °C to 4 °C when heating temperature ranged from 45 °C to 75 °C, respectively. Hydraulic stirring effectively mitigated temperature distribution, reducing the maximum temperature difference from 3 °C to 1 °C at stirring speed of 0.31 m/s when heating temperature was maintained at 55 °C. The standard deviation of the temperatures at points P1-P5 and the average temperature decreased from 0.22 at 0.11 m/s to 0.076 at 0.31 m/s. Consequently, cumulative biogas production of the MR increased from 8.085 m³ to 12.975 m³ after implementing hydraulic stirring. Microbial community analysis revealed that methanogens were more susceptible to the effects of temperature distribution compared to bacteria. This study provides guidance for practical project implementations.
作者机构：
[Liu, Hangxi; Luo, Gemiao; Deng, Zhongliang; Zou, Lintao; Wen, Piaoting; Zhou, Xuan] Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China. dzl021015@163.com;[Tang, Honghua] The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, China;[Yang, Ruifu] State Key Laboratory of Pathogen and Biosecruity, Academy of Military Medical Sciences, Beijing, China
通讯机构：
[Zhongliang Deng] D;Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China
摘要：
Hepatitis B virus (HBV) is a major pathogen posing significant challenges to global public health, making early diagnosis critical for preventing severe liver diseases. We previously developed a fluorescent biosensor named PAM-dependent dsDNA Target-activated Cas12f1 Trans Reporter (PDTCTR). However, its reliance on specialized fluorescence equipment and lack of visual readout limited its application in resource-limited settings. To address these limitations, we developed a lateral flow assay (LFA) that integrates enzymatic recombinase amplification (ERA) with the Cas12f1_ge4.1 system. This approach enables the specific amplification of the HBV target gene through ERA and leverages the precise cleavage activity of Cas12f1_ge4.1 for enhanced signal amplification. The entire detection process is completed within 50 minutes, with results readily interpretable through visual inspection. The assay achieves a minimum detection limit of 100 copies per μL and demonstrates high specificity, showing no cross-reactivity with related viruses. In a validation study involving 71 clinical samples, the system achieved a sensitivity of 94.23%, specificity of 100%, and a kappa value of 0.90 compared to quantitative PCR (qPCR), indicating high reliability. This method thus shows promise as an effective tool for early HBV diagnosis, particularly suited for rapid, on-site detection in resource-constrained environments, and holds broad potential for diverse applications.
作者机构：
[Qiuhua Zhou; Xiangsheng Tian; Yujun Ning; Yuwei Mao; Weichao Zhao; Dingxin Long] School of Public Health, Hengyang Medical School, University of South China, Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, Hengyang 421001, China;Department of nutrition, The First People's Hospital of Chenzhou, Chenzhou 423000, China;[Yiquan Ou] School of Public Health, Hengyang Medical School, University of South China, Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, Hengyang 421001, China<&wdkj&>Department of nutrition, The First People's Hospital of Chenzhou, Chenzhou 423000, China
通讯机构：
[Dingxin Long] S;School of Public Health, Hengyang Medical School, University of South China, Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, Hengyang 421001, China
摘要：
Objective To investigate whether Ginsenoside Rg1 can mitigate the adverse effects of cranial irradiation on distal reproductive function in mice and to explore the underlying mechanisms.
To investigate whether Ginsenoside Rg1 can mitigate the adverse effects of cranial irradiation on distal reproductive function in mice and to explore the underlying mechanisms.
Methods Forty male C57BL/6J mice were randomly divided to four groups [Control, irradiation (IR), IR + Rg1, Rg1), IR + Rg1 and Rg1 group treated with intraperitoneal injections of Ginsenoside Rg1 for 30 d, followed by single-dose irradiation of 5 Gy X-ray irradiation (2 Gy/min) for the IR and IR + Rg1 group. After three months, testicles, whole brain, and serum samples were collected for analysis.
Forty male C57BL/6J mice were randomly divided to four groups [Control, irradiation (IR), IR + Rg1, Rg1), IR + Rg1 and Rg1 group treated with intraperitoneal injections of Ginsenoside Rg1 for 30 d, followed by single-dose irradiation of 5 Gy X-ray irradiation (2 Gy/min) for the IR and IR + Rg1 group. After three months, testicles, whole brain, and serum samples were collected for analysis.
Results Histological staining, transmission electron microscopy, sperm analysis, and immunofluorescence demonstrated that Ginsenoside Rg1 ameliorated structural and functional damage to the testicles, enhanced sperm count (IR: 20.70 ± 1.62 vs. IR + Rg1: 33.93 ± 2.20, t = −13.23, P < 0.05), and reduced sperm malformation rates (IR: 46.33 ± 2.18 vs. IR + Rg1: 39.00 ± 1.67, t = 7.33, P < 0.05). Further Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and Enzyme linked immunosorbent assay (ELISA) assays demonstrated that Rg1 inhibited testicular apoptosis (IR: 3.21 ± 0.28 vs. IR + Rg1: 1.81 ± 0.18, t = 1.40, P < 0.05) and modulated serum testosterone (IR: 4.47 ± 0.23 vs. IR + Rg1: 6.65 ± 0.09, t = −2.18, P < 0.05), GnRH (IR: 24.37 ± 0.92 vs. IR + Rg1: 32.98 ± 1.33, t = −8.61, P < 0.05), and FSH levels (IR: 1.41 ± 0.11 vs. IR + Rg1: 2.69 ± 0.21, t = −1.28, P < 0.05). Additionally, quantitative PCR and Western blot showed that Rg1 downregulated SCF, p-PI3K, p-Akt, and mTOR protein expressions in irradiated mice.
Histological staining, transmission electron microscopy, sperm analysis, and immunofluorescence demonstrated that Ginsenoside Rg1 ameliorated structural and functional damage to the testicles, enhanced sperm count (IR: 20.70 ± 1.62 vs. IR + Rg1: 33.93 ± 2.20, t = −13.23, P < 0.05), and reduced sperm malformation rates (IR: 46.33 ± 2.18 vs. IR + Rg1: 39.00 ± 1.67, t = 7.33, P < 0.05). Further Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and Enzyme linked immunosorbent assay (ELISA) assays demonstrated that Rg1 inhibited testicular apoptosis (IR: 3.21 ± 0.28 vs. IR + Rg1: 1.81 ± 0.18, t = 1.40, P < 0.05) and modulated serum testosterone (IR: 4.47 ± 0.23 vs. IR + Rg1: 6.65 ± 0.09, t = −2.18, P < 0.05), GnRH (IR: 24.37 ± 0.92 vs. IR + Rg1: 32.98 ± 1.33, t = −8.61, P < 0.05), and FSH levels (IR: 1.41 ± 0.11 vs. IR + Rg1: 2.69 ± 0.21, t = −1.28, P < 0.05). Additionally, quantitative PCR and Western blot showed that Rg1 downregulated SCF, p-PI3K, p-Akt, and mTOR protein expressions in irradiated mice.
Conclusions Ginsenoside Rg1 potentially alleviate chronic testicular structural and functional damage by inhibiting germ cell apoptosis through the modulation of the HPG axis and the PI3K/Akt/mTOR pathway, suggesting that it is a potential therapeutic agent for reproductive disorders induced by cranial irradiation.
Ginsenoside Rg1 potentially alleviate chronic testicular structural and functional damage by inhibiting germ cell apoptosis through the modulation of the HPG axis and the PI3K/Akt/mTOR pathway, suggesting that it is a potential therapeutic agent for reproductive disorders induced by cranial irradiation.
摘要：
Nanomedicine is revolutionizing precision medicine, providing targeted, personalized treatment options. Lipid-based nanomedicines offer distinct benefits including high potency, targeted delivery, extended retention in the body, reduced toxicity, and lower required doses. These characteristics make lipid-based nanoparticles ideal for drug delivery in areas such as gene therapy, cancer treatment, and mRNA vaccines. However, traditional bulk synthesis methods for LNPs often produce larger particle sizes, significant polydispersity, and low encapsulation efficiency, which can reduce the therapeutic effectiveness. These issues primarily result from uneven mixing and limited control over particle formation during the synthesis. Microfluidic technology has emerged as a solution, providing precise control over particle size, uniformity, and encapsulation efficiency. In this mini review, we introduce the state-of-the-art microfluidic systems for lipid-based nanoparticle synthesis and functionalization. We include the working principles of different types of microfluidic systems, the use of microfluidic systems for LNP synthesis, cargo encapsulation, and nanomedicine delivery. In the end, we briefly discuss the clinical use of LNPs enabled by microfluidic devices.
