Rapid and visual detection of hepatitis B virus using the ERA/Cas12f1_ge4.1-based lateral flow assay system

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成果类型：

期刊论文

作者：

Zhou, Xuan;Tang, Honghua;Luo, Gemiao;Zou, Lintao;Liu, Hangxi;...

通讯作者：

Zhongliang Deng

作者机构：

[Liu, Hangxi; Luo, Gemiao; Deng, Zhongliang; Zou, Lintao; Wen, Piaoting; Zhou, Xuan] Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China. dzl021015@163.com

[Tang, Honghua] The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, Hunan, China

[Yang, Ruifu] State Key Laboratory of Pathogen and Biosecruity, Academy of Military Medical Sciences, Beijing, China

通讯机构：

[Zhongliang Deng] D

Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China

语种：

英文

期刊：

ANALYTICAL METHODS

ISSN：

1759-9660

年：

2025

卷：

期：

页码：

1503-1510

DOI：

10.1039/D4AY02079K

机构署名：

本校为第一且通讯机构

院系归属：

公共卫生学院

摘要：

Hepatitis B virus (HBV) is a major pathogen posing significant challenges to global public health, making early diagnosis critical for preventing severe liver diseases. We previously developed a fluorescent biosensor named PAM-dependent dsDNA Target-activated Cas12f1 Trans Reporter (PDTCTR). However, its reliance on specialized fluorescence equipment and lack of visual readout limited its application in resource-limited settings. To address these limitations, we developed a lateral flow assay (LFA) that integrates enzymatic recombinase amplific...

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Rapid and visual detection of hepatitis B virus using the ERA/Cas12f1_ge4.1-based lateral flow assay system

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