期刊论文

He, J

英文

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

2235-2988

2024

14

1454076

10.3389/fcimb.2024.1454076

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Natural Science Foundation of Hunan Province (No. 2023JJ60055, 2024JJ7459, 2024JJ9397), Health Research Project of Hunan Provincial Health Commission (No.20201915, B202311007730,20240304128), Hengyang Science and Technology Planning Project (No.202250045307).

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INTRODUCTION: Mycobacterium tuberculosis, the causative agent of human tuberculosis, poses a significant threat to global public health and imposes a considerable burden on the economy. However, existing laboratory diagnostic methods for M. tuberculosis are time-consuming and have limited sensitivity levels. METHODS: The CRISPR/Cas system, commonly known as the "gene scissors", demonstrates remarkable specificity and efficient signal amplification capabilities. Enzymatic recombinase amplification (ERA) was utilized to rapidly amplify trace DNA ...