Indole-3-acetic acid (IAA), as a critical phytohormone, has positive effects on botanic growth and development. Developed herein is a novel liquid crystal (LC) immunosensor based on the indirect competitive assay format for the detection of IAA. In light of the small molecular weight of antigen IAA, immobilising IAA on the surface of APTES/DMOAP self-assembled membranes by covalent binding has little effect on LC molecular orientation. When anti-IAA was added, anti-IAA specifically bound to immobilised-IAA on the substrate surface, forming an a...
