期刊：
International Immunopharmacology,2021年93(2):107411 ISSN：1567-5769
通讯作者：
Hu, Sihai;Long, Dingxin
作者机构：
[Wu, Xiaoxia; Hu, Sihai; Li, Zhenyu; Li, Yumeng; Hou, Yongli; Cao, Hui] Univ South China, Inst Pathogen Biol, Coll Med, Hengyang 421001, Peoples R China.;[Wang, Yan] Univ South China, Hosp 2, Operating Room, Hengyang 421001, Peoples R China.;[Long, Dingxin] Univ South China, Sch Publ Hlth, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Long, Dingxin] C;[Hu, Sihai] I;Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, China. Electronic address:;China School of Public Health, University of South China, Hengyang 421001, China. Electronic address:
关键词：
Chitosan nanoparticle;Immunopotentiators;Moucosal vaccine;NMB0315;Neisseria meningitidis serogroup B
摘要：
Neisseria meningitidis (N. meningitidis) is a human-specific pathogen and a major cause of meningitis and septicemia with a high case fatality rate. N. meningitidis may penetrate the nasopharyngeal mucosal membrane and cause severe meningitis, a mucosal immune response plays a key role in the defense against meningococcal infections. Our previous study demonstrated that N. meningitidis serogroup B 0315 (NMB0315) was a vaccine candidate against N. meningitidis serogroup B (NMB) through parenteral immunization. In this study, immunopotentiators (C48/80 or CpG-ODN) were loaded into chitosan nanoparticle (Chi NP) to form combination adjuvants (Chi-CpG NP and Chi-C48/80 NP) and adopted to enhance the immunogenicity of NMB0315 through intranasal immunization. The experimental results have indicated that both Chi-CpG NP and Chi-C48/80 NP are effective mucosal adjuvants for the induction of significantly higher rNMB0315-specific IgG, IgG1, IgG2a and sIgA antibodies. Meanwhile, Chi-CpG NP and Chi-C48/80 NP could change the ratio of IgG1/IgG2a, inducing a more balanced cellular/humoral immune response. Chi-CpG NP and Chi-C48/80 NP also boosted interleukin-4 (IL-4), interferon-γ (IFN-γ) and interleukin-17 A (IL-17A) production by splenocytes. The bactericidal antibodies have been detected in sera from mice immunized with rNMB0315+Chi-CpG NP and rNMB0315+Chi-C48/80 NP. Overall, the combination adjuvants could be applicable to the development of a mucosal vaccine against NMB.
作者机构：
[Yuan, Shunling; Liu, Li; Liu, Zhaoping; Wang, Yanyan; Zhang, Ji; Hu, Dingyu] Univ South China, Affiliated Hosp 1, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Ji] Shenzhen Tradit Chinese Med Hosp, Dept Clin Lab, Shenzhen 518033, Guangdong, Peoples R China.;[Zhong, Jing] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.;[Wen, Feng; Li, Junjun] Univ South China, Affiliated Hosp 1, Dept Hematol, Hengyang 421001, Hunan, Peoples R China.;[Liu, Jing] Cent South Univ, Sch Life Sci, Hunan Prov Key Lab Basic & Appl Hematol, Mol Biol Res Ctr, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Jing Liu] H;[Ji Zhang] D;Department of Clinical Laboratory, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Guangdong, China<&wdkj&>Department of Clinical Laboratory, The First Affiliated Hospital, University of South China, Hengyang 421001, Hunan, China<&wdkj&>Hunan Province Key Laboratory of Basic and Applied Hematology, Molecular Biology Research Center & Center for Medical Genetics, School of Life Sciences, Central South University, Changsha 410078, Hunan, China
摘要：
Cellular senescence constitutes a permanent state of cell cycle arrest in proliferative cells induced by different stresses. The exploration of tumor pathogenesis and therapies has been a research hotspot in recent years. Cellu-lar senescence is a significant mechanism to prevent the proliferation of potential tumor cells, but it can also pro-mote tumor growth. Increasing evidence suggests that cellular senescence is involved in the pathogenesis and development of hematological malignancies, including leukemia, myelodysplastic syndrome (MDS) and multiple myeloma (MM). Cellular senescence is associated with functional decline of hematopoietic stem cells (HSCs) and increased risk of hematological malignancies. Moreover, the bone marrow (BM) microenvironment has a crucial regulatory effect in the process of these diseases. The senescence-associated secretory phenotype (SASP) in the BM microenvironment establishes a protumor environment that supports the proliferation and survival of tumor cells. Therefore, a series of therapeutic strategies targeting cellular senescence have been gradually devel-oped, including the induction of cellular senescence and elimination of senescent cells. This review systematically summarizes the emerging information describing the roles of cellular senescence in tumorigenesis and potential clinical applications, which may be beneficial for designing rational therapeutic strategies for various hematopoi-etic malignancies. (c) 2021 Elsevier Inc. All rights reserved.
作者机构：
[Wu, Zhijian; Zhang, Jian; Liu, Ziyan; Jin, Kun; Huang, Renbin; Li, Guicheng; Wu, Qian; Qu, Xiaowang; Niu, Ling; Lei, Dongzhu; Hu, Yabin; Lin, Yingbiao; Guo, Wangyuan; Luo, Dixian; Liu, Wenpei] Univ South China, Peoples Hosp Chenzhou 1, Translat Med Inst, Chenzhou, Peoples R China.;[Xie, Ting; Peng, Danhong; Huang, Mincheng; He, Shuangyan; Xie, Xiangping; Zeng, Ting; Wang, Qijie] Cent Hosp Shaoyang, Shaoyang, Peoples R China.;[Huang, Weijin; Wu, Jiajing; Wang, Youchun] Natl Inst Food & Drug Control, Minist Hlth Res Qual & Standardizat Biotech Prod, Key Lab Biol Prod Qual Res & Evaluat, Natl Med Prod Adm,Key Lab, Beijing, Peoples R China.;[Wu, Jiajing] Wuhan Inst Biol Prod, Wuhan, Peoples R China.;[Bai, Tingting; Liu, Wenpei] Southern Med Univ, Sch Clin Med 1, Guangzhou, Peoples R China.
通讯机构：
[Liu, WP; Qu, XW] U;[Wang, Youchun] N;[Liu, Wenpei; Li, Yi-Ping; Qu, Xiaowang] S;Univ South China, Peoples Hosp Chenzhou 1, Translat Med Inst, Chenzhou, Peoples R China.;Natl Inst Food & Drug Control, Minist Hlth Res Qual & Standardizat Biotech Prod, Key Lab Biol Prod Qual Res & Evaluat, Natl Med Prod Adm,Key Lab, Beijing, Peoples R China.
摘要：
Coronavirus disease 2019 (COVID-19) is caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)(1-3) and individuals with COVID-19 have symptoms that can be asymptomatic, mild, moderate or severe(4,5). In the early phase of infection, T- and B-cell counts are substantially decreased(6,7); however, IgM(8-11) and IgG(12-14) are detectable within 14 d after symptom onset. In COVID-19-convalescent individuals, spike-specific neutralizing antibodies are variable(3,15,16). No specific drug or vaccine is available for COVID-19 at the time of writing; however, patients benefit from treatment with serum from COVID-19-convalescent individuals(17,18). Nevertheless, antibody responses and cross-reactivity with other coronaviruses in COVID-19-convalescent individuals are largely unknown. Here, we show that the majority of COVID-19-convalescent individuals maintained SARS-CoV-2 spike S1- and S2-specific antibodies with neutralizing activity against the SARS-CoV-2 pseudotyped virus, and that some of the antibodies cross-neutralized SARS-CoV, Middle East respiratory syndrome coronavirus or both pseudotyped viruses. Convalescent individuals who experienced severe COVID-19 showed higher neutralizing antibody titres, a faster increase in lymphocyte counts and a higher frequency of CXCR3(+) T follicular help (T-FH) cells compared with COVID-19-convalescent individuals who experienced non-severe disease. Circulating T-FH cells were spike specific and functional, and the frequencies of CXCR3(+) T-FH cells were positively associated with neutralizing antibody titres in COVID-19-convalescent individuals. No individuals had detectable autoantibodies. These findings provide insights into neutralizing antibody responses in COVID-19-convalescent individuals and facilitate the treatment and vaccine development for SARS-CoV-2 infection.
期刊：
Frontiers in Genetics,2021年11:571403 ISSN：1664-8021
通讯作者：
Yang, Jing;Liu, Zhifeng
作者机构：
[Hu, Guangsheng; Yang, Jing] Univ South China, Affiliated Hosp 1, Dept Gastroenterol, Hengyang, Peoples R China.;[Peng, Hong; Liu, Zhifeng; Yu, Jing; Jiang, Qingshan; Tang, Yanhua; Li, Shuyan; Liu, Lijun; Wang, Yaya] Univ South China, Affiliated Hosp 1, Dept Otorhinolaryngol, Hengyang, Peoples R China.;[Yang, Jing] Univ South China, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Canc Res Inst, Hengyang, Peoples R China.
通讯机构：
[Yang, Jing; Liu, Zhifeng] U;Univ South China, Affiliated Hosp 1, Dept Gastroenterol, Hengyang, Peoples R China.;Univ South China, Affiliated Hosp 1, Dept Otorhinolaryngol, Hengyang, Peoples R China.;Univ South China, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Canc Res Inst, Hengyang, Peoples R China.
关键词：
Squamous cell carcinoma of head and neck (SCCHN);RNA binding proteins (RBPs);Differentially Expressed Genes (DEGs);prognosis;tumor immunity
摘要：
RNA-binding proteins (RBPs) interacting with target RNAs play essential roles in RNA metabolism at the post-transcription level. Perturbations of RBPs can accelerate cancer development and cause dysregulation of the immune cell function and activity leading to evade immune destruction of cancer cells. However, few studies have systematically analyzed the potential prognostic value and functions of RBPs in squamous cell carcinoma of head and neck (SCCHN). Here, for the first time, we comprehensively identified 92 differentially expressed RBPs from The Cancer Genome Atlas (TCGA) database. In the training set, a prognosis risk model was constructed with six RBPs, including NCBP2, MKRN3, MRPL47, AZGP1, IGF2BP2, and EZH2, and validated by the TCGA test set, the TCGA all set, and the GEO data set. In addition, the risk score was related to the clinical stage, T classification, and N classification. Furthermore, the high-risk score was significantly correlated with immunosuppression, and low expression of EZH2 and AZGP1 and high expression of IGF2BP2 were the main factors. Thus, the risk model may serve as a prognostic signature and offer highlights for individualized immunotherapy in SCCHN patients.
摘要：
As an evolutionarily conserved intracellular degradation pathway, autophagy is essential to cellular homeostasis. Several studies have demonstrated that autophagy showed an important effect on some pulmonary fibrosis diseases, including idiopathic pulmonary fibrosis (IPF), cystic fibrosis lung disease, silicosis and smoking-induced pulmonary fibrosis. For example, autophagy mitigates the pathological progression of IPF by regulating the apoptosis of fibroblasts and the senescence of alveolar epithelial cells. In addition, autophagy ameliorates cystic fibrosis lung disease via rescuing transmembrane conductance regulators (CFTRs) to the plasma membrane. Furthermore, autophagy alleviates the silica-induced pulmonary fibrosis by decreasing apoptosis of alveolar epithelial cells in silicosis. However, excessive macrophage autophagy aggravates the pathogenesis of silicosis fibrosis by promoting the proliferation and migration of lung fibroblasts in silicosis. Autophagy is also involved in smoking-induced pulmonary fibrosis, coal workers' pneumoconiosis, ionizing radiation-mediated pulmonary fibrosis and heavy metal nanoparticle-mediated pulmonary fibrosis. In this review, the role and signalling mechanisms of autophagy in the progression of pulmonary fibrosis diseases have been systematically analysed. It has provided a new insight into the therapeutic potential associated with autophagy in pulmonary fibrosis diseases. In conclusion, the targeting of autophagy might prove to be a prospective avenue for the therapeutic intervention of pulmonary fibrosis diseases.
摘要：
<jats:title>Abstract</jats:title>
<jats:p>Colorectal cancer (CRC) is the third most common malignancy and one of the leading causes of cancer-related death among men worldwide. CRC is a multifactor digestive pathology, which is a huge problem faced not only by clinicians but also by researchers. Importantly, a unique feature of CRC is the dysregulation of molecular signaling pathways. To date, a series of reviews have indicated that different signaling pathways are disordered and have potential as therapeutic targets in CRC. Nevertheless, an overview of the function and interaction of multiple signaling pathways in CRC is needed. Therefore, we summarized the pathways, biological functions and important interactions involved in CRC. First, we investigated the involvement of signaling pathways, including Wnt, PI3K/Akt, Hedgehog, ErbB, RHOA, Notch, BMP, Hippo, AMPK, NF-κB, MAPK and JNK. Subsequently, we discussed the biological function of these pathways in pathophysiological aspects of CRC, such as proliferation, apoptosis and metastasis. Finally, we summarized important interactions among these pathways in CRC. We believe that the interaction of these pathways could provide new strategies for the treatment of CRC.</jats:p>
摘要：
Listeria ivanovii subsp. ivanovii is an intracellular bacterium distributed widely in nature, causing the listeriosis in ruminants and humans. Previous researches had isolated 116 strains of L. ivanovii subsp. ivanovii from wild rodents and pikas of different regions in China, and the predominant sequence types were ST1 and ST2. In this study, we first investigated the biological characteristics and virulence of these two clonal strains including motility, metabolism and virulence in cells and mouse model. The results demonstrated the ST1 strains exhibited motility, wide metabolic activity and hypervirulence, whereas the ST2 strains showed non-motility, relative lower metabolic activity and virulence. Considering the transmissible ability from wild rodents and pikas to ecological environment, the L. ivanovii subsp. ivanovii with potential pathogenicity to humans and ruminants should be monitored.
摘要：
Introduction: This meta-analysis aimed to explore the preventive effects of combined statin and antihypertensive therapy on major cardiovascular outcomes in patients with hypertension. Methods: PubMed, Embase, and the Cochrane Library databases and reference lists of published studies were systematically searched throughout October 9, 2019. Studies designed as randomized controlled trials and investigating the effects of combined statin and antihypertensive therapy versus antihypertensive therapy alone were included. Data abstraction and quality of included studies were assessed by 2 independent authors. The summary results were calculated using relative risks (RRs) with 95% CIs employing a random-effects model. Results: A total of 8 randomized controlled trials including 38,618 patients were finally enrolled. The summary RRs indicated that the combined therapy significantly reduced the risk of major adverse cardiovascular events compared with antihypertensive therapy alone (RR 0.79; 95% CI 0.71–0.88; p < 0.001). Furthermore, the patients in the combined therapy group also experienced less myocardial infarction (RR 0.67; 95% CI 0.53–0.84; p = 0.001) and stroke risks (RR 0.82; 95% CI 0.72–0.94; p = 0.005), while no significant difference was observed between combined therapy and antihypertensive therapy alone regarding cardiac death (RR 0.96; 95% CI 0.84–1.08; p = 0.465) and all-cause mortality (RR 0.95; 95% CI 0.86–1.04; p = 0.277). Conclusion: These findings suggested that combined statin and antihypertensive therapy was associated with more cardiovascular benefits compared with antihypertensive therapy alone.
期刊：
Biochemical and Biophysical Research Communications,2019年508(1):97-101 ISSN：0006-291X
通讯作者：
Ouyang, Xin-Ping;Tang, Chao-Ke
作者机构：
[Liu, Juan; He, Ping-Ping; Zou, Jie-Qiong; Shen, Qian-Qian; Wang, Yan; Wen, Min; Chen, Ye-Shi; Yang, Jiao-Xing; Su, Hua; Hu, Li-Zhi] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[He, Ping-Ping; Ouyang, Xin-Ping; Tang, Chao-Ke] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Liu, Juan; Zou, Jie-Qiong; Wang, Yan; Yang, Jiao-Xing; Hu, Li-Zhi] Univ South China, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Zheng, Xi -Long] Univ Calgary, Dept Biochem & Mol Biol, Libin Cardiovasc Inst Alberta, Cumming Sch Med,Hlth Sci Ctr, 3330 Hosp Dr NW, Calgary, AB T2N 4N1, Canada.;[Ouyang, Xin-Ping] Univ South China, Dept Physiol, Neurosci Inst, Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Ouyang, Xin-Ping; Tang, Chao-Ke] U;Univ South China, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
*Atherosclerosis;*LPL;*MiR-590;*Nobiletin
摘要：
Nobiletin has protective effects on cardiovascular diseases, but the mechanism is not clear. In this study, we examined whether nobiletin affects the expression of miR-590/LPL and its relative effects on lipid accumulation and pro-inflammatory cytokine secretion in human THP-1 macrophages. RT-qPCR analysis showed that nobiletin increased the expression of miR-590. Western blot analysis showed that nobiletin-suppressed LPL expression was enhanced by miR-590 mimic and abrogated by miR-590 inhibitor. Oil Red 0 staining and high-performance liquid chromatography assays showed that nobiletin attenuated lipid accumulation in macrophages. Treatment with nobiletin and miR-590 mimic decreased cellular lipid accumulation, whereas treatment with miR-590 inhibitor increased cellular lipid accumulation. ELISA illustrated that nobiletin alleviated pro-inflammatory cytokine secretion in macrophages as measured by, which was reduced by miR-590 mimic and increased by miR-590 inhibitor. In conclusion, nobiletin may alleviate lipid accumulation and secretion of pro-inflammatory cytokines by enhancing the inhibitory effect of miR-590 on LPL expression, suggesting a promising strategy for potential drug development for atherosclerosis. (C) 2018 Elsevier Inc. All rights reserved.
摘要：
Extracellular vesicles (EVs), which exist in human blood, are increased in some inflammation-related cardiovascular diseases. EVs are involved in inflammation, immunity, signal transduction, cell survival and apoptosis, angiogenesis, thrombosis, and autophagy, all of which are highly significant for maintaining homeostasis and disease progression. Therefore, EVs are also associated with key steps in atherosclerosis, including cellular lipid metabolism, endothelial dysfunction and vascular wall inflammation, ultimately resulting in vascular remodelling. In this review, we summarize recent studies on EV contents and biological function, focusing on their potential effect in atherosclerosis, including cholesterol metabolism, vascular inflammation, angiogenesis, coagulation and the development of atherosclerotic lesions. EVs may represent potential biomarkers and pharmacological targets for atherosclerotic diseases.
摘要：
BACKGROUND: Salinomycin is a monocarboxylic polyether antibiotic and is a potential chemotherapy drug. Our previous studies showed that salinomycin inhibited cell growth and targeted CSCs in prostate cancer. However, the precise target of salinomycin action is unclear. METHODS: In this work, we analyzed and identified differentially expressed genes (DEGs) after treatment with or without salinomycin using a gene expression microarray in vitro (PC-3 cells) and in vivo (NOD/SCID mice xenograft model generated from implanted PC-3 cells). Western blotting and immunohistochemical staining were used to analyze the expression of ATP2A3 and endoplasmic reticulum (ER) stress biomarkers. Flow cytometry was used to analyze the cell cycle, apoptosis and intracellular Ca(2+) concentration. RESULTS: A significantly upregulated gene, ATPase sarcoplasmatic/endoplasmatic reticulum Ca(2+) transporting 3 (ATP2A3), was successfully identified. In subsequent studies, we found that ATP2A3 overexpression could trigger ER stress and exert anti-cancer effects in PC-3 and DU145 cells. ATP2A3 was slightly expressed, but the ER stress biomarkers showed strong staining in prostate cancer tissues. We also found that salinomycin could trigger ER stress, which might be related to ATP2A3-mediated Ca(2+) release in PC-3 cells. Furthermore, we found that salinomycin-triggered ER stress could promote apoptosis and thus exert anti-cancer effects in prostate cancer cells. CONCLUSION: This study demonstrates that ATP2A3 might be one of the potential targets for salinomycin, which can inhibit Ca(2+) release and trigger ER stress to exert anti-cancer effects.
