MicroRNA expression profiling and target gene analysis in gastric cancer

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Xu, Chenguang;Xie, Juan;Liu, Yanping;Tang, Fenfen;Long, Zhi;...

通讯作者：

Li, Guoqing

作者机构：

[Tang, Fenfen; Long, Zhi; Wang, Yaodong; Xu, Chenguang; Liu, Yanping; Li, Guoqing] Univ South China, Affiliated Hosp 2, Dept Gastroenterol, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.

[Xu, Chenguang; Li, Junda] Peoples Hosp Longhua Dist, Dept Gastroenterol, Shenzhen, Peoples R China.

[Xie, Juan] Univ South China, Hengyang Med Coll, Res Lab Translat Med, Hengyang, Peoples R China.

[Xie, Juan] Univ South China, Hengyang Med Coll, Inst Pharm & Pharmacol, Hengyang, Peoples R China.

[Luo, Jiangyan] Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Dept Ultrasonog, Qingyuan, Peoples R China.

通讯机构：

[Li, Guoqing] U

Univ South China, Affiliated Hosp 2, Dept Gastroenterol, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.

语种：

英文

关键词：

bioinformatics analysis;gastric cancer;microRNA;microRNA expression microarray;qRT-PCR

期刊：

MEDICINE

ISSN：

0025-7974

年：

2020

卷：

期：

页码：

e21963

DOI：

10.1097/MD.0000000000021963

基金类别：

Scientific Research Project of Hunan Provincial Health Commission [A2017014]; Postgraduate Research Innovation Project of Hunan Province [CX2018B645]

机构署名：

本校为第一且通讯机构

院系归属：

医学院

药学与生物科学学院

摘要：

This study aims to identify differentially expressed microRNAs (miRNAs) in gastric cancer by comparing gastric cancerous tissues with normal tissues, explore the potential roles.The miRNA expression microarray was employed on gastric cancer tissues, and apparently normal para-cancerous tissues from 3 patients undergoing radical surgery were matched. Quantitative RT-PCR was performed on the other 7 patients to validate the findings of the microarray. Furthermore, Gene Ontology (GO) analysis and enrichment analysis of KEGG Pathway were performed for 5 dysregulated candidate miRNAs, including 3 u...

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MicroRNA expression profiling and target gene analysis in gastric cancer

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