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A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains

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成果类型:
期刊论文
作者:
Zhou, Zhou;Liu, Na;Wang, Yingzi;Emmanuel, Arthur Wirekoh;You, Xiaoxing;...
通讯作者:
Wu, Yimou
作者机构:
[Li, Zhongyu; Zhou, Zhou; Wu, Yimou; Liu, Na; You, Xiaoxing; Wang, Yingzi; Zhong, Guangming; Emmanuel, Arthur Wirekoh] Univ South China, Coll Med, Inst Pathogen Biol, Hengyang 421001, Hunan, Peoples R China.
[Li, Zhongyu; Zhou, Zhou; Wu, Yimou; Liu, Na; You, Xiaoxing; Wang, Yingzi; Emmanuel, Arthur Wirekoh] Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Hunan, Peoples R China.
[Liu, Jiulin] Univ South China, Coll Med, Outstanding Phys Class Grade 2016, Hengyang 421001, Hunan, Peoples R China.
[Zhong, Guangming] Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA.
[Wu, Yimou] Univ South China, Pathogen Biol Inst, 28 Changsheng Western Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Wu, Yimou] U
Univ South China, Pathogen Biol Inst, 28 Changsheng Western Rd, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
bacterial RNA;RNA 16S;animal experiment;animal model;Article;bacterial gene;bacterial growth;bacterial mutation;bacterial strain;bacterial virulence;bacterium isolation;cell inclusion;Chlamydia muridarum;chlamydial urethritis;clone;comparative study;controlled study;female;gene expression;genital tract infection;genotype;HeLa cell line;human;human cell;hydrosalpinx;in vitro study;male;mouse;nonhuman;plaque forming cell assay;point mutation;priority journal;whole genome sequencing;animal;bacterial load;C3H mouse;Chlamydia muridarum;chlamydiasis;disease model;genetic variation;genetics;growth, development and aging;microbiology;mutation;pathogenicity;pathology;vagina;virulence;virus culture;Animals;Bacterial Load;Chlamydia Infections;Chlamydia muridarum;Disease Models, Animal;Female;Genes, Bacterial;Genetic Variation;Genotype;HeLa Cells;Humans;Mice, Inbred C3H;Mutation;Reproductive Tract Infections;Serial Passage;Vagina;Virulence;Whole Genome Sequencing
期刊:
Pathogens and Disease
ISSN:
0928-8244
年:
2019
卷:
77
期:
3
页码:
-
基金类别:
This work was supported by grants from the National Natural Science Foundation of China (31570179 and 30901352), the Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study (2015–351), the Foundation of Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control (No. 2014–5) and the Construct Program of the Key Discipline in Hunan Province (2011–76).
机构署名:
本校为第一且通讯机构
院系归属:
医学院
摘要:
Objective: This study is to investigate the functions of newly discovered genes in Chlamydia muridarum (C. muridarum) strains with single gene differences. Methods: Using whole genome sequencing and plaque formation assays, C. muridarum parental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to the in vitro and in vivo ex...

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