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An enzymatic on/off switch-mediated assay for KRAS hotspot point mutation detection of circulating tumor DNA

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成果类型:
期刊论文
作者:
Wang, Qing-lin;Zhou, Cui-lan;Yin, Yu-fang;Xiao, Li;Wang, Yuan*;...
通讯作者:
Wang, Yuan;Li, Kai
作者机构:
[Li, Kai; Wang, Qing-lin; Wang, Yuan] Soochow Univ, Jiangsu Key Lab Neuropsychiat Dis, Suzhou 215123, Jiangsu, Peoples R China.
[Li, Kai; Wang, Qing-lin; Wang, Yuan; Li, K] Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Jiangsu, Peoples R China.
[Zhou, Cui-lan] Univ South China, Dept Human Anat, Hengyang, Peoples R China.
[Yin, Yu-fang] SIU Med Sch, Dept Pharmacol & Neurosci, Springfield, IL USA.
[Xiao, Li] Soochow Univ, Lab Mol Med, Affiliated Hosp 2, Suzhou, Peoples R China.
通讯机构:
[Wang, Yuan; Wang, Y; Li, K] S
[Li, Kai] G
Soochow Univ, Jiangsu Key Lab Neuropsychiat Dis, Suzhou 215123, Jiangsu, Peoples R China.
Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Jiangsu, Peoples R China.
GeneTalks Biotechnol Inc, Changsha 410013, Hunan, Peoples R China.
语种:
英文
关键词:
circulating tumor DNA;DNA fragment;DNA polymerase;exonuclease;exonuclease polymerase;unclassified drug;circulating tumor DNA;KRAS protein, human;protein p21;Article;cancer diagnosis;cancer patient;clinical article;colorectal cancer;controlled study;cost effectiveness analysis;diagnostic accuracy;diagnostic test accuracy study;DNA determination;dna mutational analysis;exonuclease polymerase mediated on off switch;gel electrophoresis;high risk population;human;intermethod comparison;multiplex real time polymerase chain reaction;non invasive procedure;oncogene K ras;point mutation;Sanger sequencing;sensitivity and specificity;somatic mutation;blood;colorectal tumor;dna mutational analysis;genetics;mortality;point mutation;procedures;real time polymerase chain reaction;Circulating Tumor DNA;Colorectal Neoplasms;DNA Mutational Analysis;Humans;Point Mutation;Proto-Oncogene Proteins p21(ras);Real-Time Polymerase Chain Reaction;Sensitivity and Specificity
期刊:
Journal of Clinical Laboratory Analysis
ISSN:
0887-8013
年:
2020
卷:
34
期:
8
页码:
e23305-
基金类别:
This study was supported by The Priority Academic Program Development of the Jiangsu Higher Education Institutes. And we acknowledge The Jiangsu Province Youth Medical Talent Support Program (QNRC2016868), and The Suzhou Science and Technology Development Program (SZS201618, SYS201727).
机构署名:
本校为其他机构
院系归属:
医学院
摘要:
Background: To detect the mutations of KRAS gene in colorectal cancer patients and other cancer patients, it is of value to develop non-invasive, sensitive, specific, easy, and low-cost assays. Methods: Templates harboring hotspot mutations of the KRAS gene were constructed, and primers were designed for evaluation of the specificity, and sensitivity of detection system consisted of exonuclease polymerase-mediated on/off switch; then, gel electrophoresis and real-time PCR were performed for verification. The assay was verified by testing the DN...

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