作者： Chen, Menglong;Wang, Liang;Li, Yaqin;Chen, Yongjun;Zhang, Huili;...

期刊： FRONTIERS IN NEUROLOGY,2020年11:528704 ISSN：1664-2295

通讯作者： Zhang, Cheng;Zhang, Yu

作者机构： [Zhang, Cheng; Lin, Jinfu; Li, Huan; Wang, Liang; Zhu, Yuling; Chen, Menglong; He, Ruojie] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Neurol, Guangzhou, Peoples R China.;[Chen, Menglong] Natl Key Clin Dept, Guangdong Prov Key Lab Diag & Treatment Major Neu, Guangzhou, Peoples R China.;[Chen, Menglong] Key Discipline Neurol, Guangzhou, Peoples R China.;[Zhang, Yu; Chen, Menglong] Jinan Univ, Affiliated Hosp 1, Dept Neurol, Guangzhou, Peoples R China.;[Li, Yaqin] Sun Yat Sen Univ, Affiliated Hosp 7, Dept Neurol, Shenzhen, Peoples R China.

通讯机构： [Zhang, Cheng] S;[Zhang, Yu] J;Sun Yat Sen Univ, Affiliated Hosp 1, Dept Neurol, Guangzhou, Peoples R China.;Jinan Univ, Affiliated Hosp 1, Dept Neurol, Guangzhou, Peoples R China.

关键词： Duchenne muscular dystrophy;genetic modifiers;LTBP4;single nucleotide polymorphisms;SPP1

摘要： Background: Duchenne muscular dystrophy (DMD) is a fatal, X-linked recessive muscle disorder characterized by heterogeneous progression and severity. We aimed to study the effects of single nucleotide polymorphisms (SNPs) in SPP1 and LTBP4 on DMD progression in Chinese patients. Methods: We genotyped LTBP4 haplotypes and the SPP1 promoter SNPs rs28357094, rs11730582, and rs17524488 in 326 patients registered in the neuromuscular database of The First Affiliated Hospital of Sun Yat-sen University. Kaplan-Meier curves and log-rank tests were used to estimate and compare median age at loss of ambulation, while Cox proportional hazard regression models were used as to analyze the effects of glucocorticoids treatments, DMD genotype, and SPP1/LTBP4 SNPs on loss of ambulation. Results: The CC/CT genotype at rs11730582 was associated with a 1.33-year delay in ambulation loss (p = 0.006), with hazard ratio 0.63 (p = 0.008), in patients with truncated DMD genotype and undergoing steroid treatment. On the other hand, rs17524488 in SPP1 and the IAAM/IAAM haplotype in LTBP4 were not associated with time to ambulation loss. Conclusions: SPP1 rs11730582 is a genetic modifier of the long-term effects of steroid treatment in Chinese DMD patients. Thus, any future clinical study in DMD should adjust for glucocorticoids use, DMD genotype, and SPP1 polymorphisms. © Copyright © 2020 Chen, Wang, Li, Chen, Zhang, Zhu, He, Li, Lin, Zhang and Zhang.

语种： 英文

作者： 曾莎莎;肖玲巧;唐瑶;段洁;张洁雅;...

期刊： 中南医学科学杂志,2020年48(06):618-623 ISSN：2095-1116

作者机构： 南华大学衡阳医学院病原生物学研究所,特殊病原体防控湖南省重点实验室,湖南 衡阳421001;南华大学护理学院,湖南 衡阳421001;[曾莎莎; 郭开云; 肖玲巧; 唐瑶; 段洁; 张洁雅; 张艳; 李蕊] 南华大学

关键词： 幽门螺杆菌;肿瘤坏死因子α诱导蛋白（Tipα）;Wnt/β-catenin信号通路;胃癌;上皮-间质转化（EMT）

摘要： 探讨幽门螺杆菌(H.pylori)肿瘤坏死因子α诱导蛋白(Tipα)是否通过Wnt/β联蛋白(β-catenin)信号通路诱导人胃癌SGC7901细胞上皮-间质转化(EMT).设置PBS组、Tipα组和通路抑制剂XAV939预处理组,采用Western blot和免疫荧光观察β-catenin磷酸化和核转位情况;qRT-PCR和Western blot检测E-钙黏蛋白(E-cad-herin)、紧密连接蛋白(ZO-1)、N-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)以及通路下游靶基因(c-myc和cyclinD1)的表达情况;划痕实验和Transwell实验检测细胞迁移能力.结果显示,Tipα能以时间依赖性方式诱导β-catenin Ser675和Ser552磷酸化和核转位,且下调E-cadherin和ZO-1表达,上调N-cadherin和Vimentin表达(P<0.01);与PBS相比,Tipα增强c-myc和cyclinD1的表达(P<0.05)以及细胞的迁移能力(P<0.05).XAV939预处理抑制Tipα诱导的SGC7901细胞EMT形成及通路下游靶基因的表达.表明Wnt/β-catenin信号通路参与了Tipα诱导的胃癌细胞EMT形成.

语种： 中文

作者： Hu, Ke;Xiang, Ju*;Yu, Yun-Xia*;Tang, Liang;Xiang, Qin;...

期刊： PLOS ONE,2020年15(3):e0227244 ISSN：1932-6203

通讯作者： Yu, Yun-Xia;Xiang, Ju

作者机构： [Yu, Yun-Xia; Hu, Ke] Xiangtan Univ, Sch Phys & Optoelect Engn, Xiangtan, Hunan, Peoples R China.;[Xiang, Ju; Zhang, Yan] Cent South Univ, Sch Comp Sci & Engn, Changsha, Hunan, Peoples R China.;[Li, Jian-Ming; Xiang, Qin; Xiang, Ju; Zhang, Yan; Tang, Liang] Changsha Med Univ, Sch Basic Med Sci, Changsha, Hunan, Peoples R China.;[Li, Jian-Ming] Cent South Univ, Xiang Ya Hosp, Dept Neurol, Changsha, Hunan, Peoples R China.;[Li, Jian-Ming] Xiangya Boai Rehabil Hosp, Dept Rehabil, Changsha, Hunan, Peoples R China.

通讯机构： [Yu, Yun-Xia] X;[Xiang, Ju] C;Xiangtan Univ, Sch Phys & Optoelect Engn, Xiangtan, Hunan, Peoples R China.;Cent South Univ, Sch Comp Sci & Engn, Changsha, Hunan, Peoples R China.;Changsha Med Univ, Sch Basic Med Sci, Changsha, Hunan, Peoples R China.

摘要： Community detection in complex networks is an important issue in network science. Several statistical measures have been proposed and widely applied to detecting the communities in various complex networks. However, due to the lack of flexibility resolution, some of them have to encounter the resolution limit and thus are not compatible with multi-scale structures of complex networks. In this paper, we investigated a statistical measure of interest for community detection, Significance [Sci. Rep. 3 (2013) 2930], and analyzed its critical behaviors based on the theoretical derivation of critical number of communities and the phase diagram in community-partition transition. It was revealed that Significance exhibits far higher resolution than the traditional Modularity when the intra- and inter-link densities of communities are obviously different. Following the critical analysis, we developed a multi-resolution version of Significance for identifying communities in the multi-scale networks. Experimental tests in several typical networks have been performed and confirmed that the generalized Significance can be competent for the multi-scale communities detection. Moreover, it can effectively relax the first- and second-type resolution limits. Finally, we displayed an important potential application of the multi-scale Significance in computational biology: disease-gene identification, showing that extracting information from the perspective of multi-scale module mining is helpful for disease gene prediction.

语种： 英文

作者： Zhang, Yunsheng;Li, Fang;Liu, Luogen;Jiang, Hongtao;Hu, Hua;...

期刊： BMC Cancer,2019年19(1):1-13 ISSN：1471-2407

通讯作者： Wang, Yi

作者机构： [Wang, Yi; Zhang, Yunsheng; Ge, Xin; Liu, Luogen] Univ South China, Clin Res Inst, Affiliated Hosp 2, Hengyang 421001, Peoples R China.;[Zhang, Yunsheng] Clin Res Ctr Breast & Thyroid Dis Prevent Hunan P, Hengyang 421001, Peoples R China.;[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.;[Jiang, Hongtao] Univ South China, Dept Urol, Hosp 2, Hengyang 421001, Peoples R China.;[Hu, Hua] Univ South China, Canc Res Inst, Hosp 2, Hengyang 421001, Peoples R China.

通讯机构： [Wang, Yi] U;[Wang, Yi] H;Univ South China, Clin Res Inst, Affiliated Hosp 2, Hengyang 421001, Peoples R China.;Hainan Med Univ, Dept Urol, Affiliated Hosp 2, Haikou 570102, Hainan, Peoples R China.

关键词： Salinomycin;ATP2A3;ER stress;Ca2+ release;Apoptosis

摘要： Background: Salinomycin is a monocarboxylic polyether antibiotic and is a potential chemotherapy drug. Our previous studies showed that salinomycin inhibited cell growth and targeted CSCs in prostate cancer. However, the precise target of salinomycin action is unclear. Methods: In this work, we analyzed and identified differentially expressed genes (DEGs) after treatment with or without salinomycin using a gene expression microarray in vitro (PC-3 cells) and in vivo (NOD/SCID mice xenograft model generated from implanted PC-3 cells). Western blotting and immunohistochemical staining were used to analyze the expression of ATP2A3 and endoplasmic reticulum (ER) stress biomarkers. Flow cytometry was used to analyze the cell cycle, apoptosis and intracellular Ca2+ concentration. Results: A significantly upregulated gene, ATPase sarcoplasmatic/endoplasmatic reticulum Ca2+ transporting 3 (ATP2A3), was successfully identified. In subsequent studies, we found that ATP2A3 overexpression could trigger ER stress and exert anti-cancer effects in PC-3 and DU145 cells. ATP2A3 was slightly expressed, but the ER stress biomarkers showed strong staining in prostate cancer tissues. We also found that salinomycin could trigger ER stress, which might be related to ATP2A3-mediated Ca2+ release in PC-3 cells. Furthermore, we found that salinomycin-triggered ER stress could promote apoptosis and thus exert anti-cancer effects in prostate cancer cells. Conclusion: This study demonstrates that ATP2A3 might be one of the potential targets for salinomycin, which can inhibit Ca2+ release and trigger ER stress to exert anti-cancer effects. © 2019 The Author(s).

语种： 英文

作者： 韩亮(综述);张艳(审校)

期刊： 微生物学免疫学进展,2019年47(3):81-85 ISSN：1005-5673

作者机构： 南华大学衡阳医学院病原生物学研究所,湖南 衡阳,421001

关键词： 胃癌;幽门螺杆菌;信号通路;上皮间质转化

摘要： 胃癌(gastric cance, GC)是世界范围内最常见的恶性肿瘤之一,其病死率在癌症中位居第二。GC的发病机制目前尚不明确,其发病机制与多种因素有关,其中包括环境因素和遗传因素。幽门螺杆菌( Helicobacter pylori , Hp)感染是GC发生最为重要的环境因素之一。Hp感染在GC的发展过程中,会伴有一些基因和信号通路的异常。现就Hp感染引发GC过程中相关信号通路的异常,包括Hedgehog信号通路、Notch信号通路、Wnt/β-catenin信号通路以及上皮间质转化(epithelial-mesenchymal transition, EMT)相关信号通路等作一概述,为GC的预防及靶向治疗提供理论依据。

语种： 中文

作者： 李杰;陈国栋;曾莎莎;肖灵巧;韩亮;...

期刊： 中国病原生物学杂志,2019年14(09):1024-1028 ISSN：1673-5234

作者机构： 永州职业技术学院生物化学教研室,湖南永州425006;南华大学衡阳医学院病原生物学研究所;[曾莎莎; 韩亮; 段洁; 张洁雅; 肖灵巧; 张艳; 陈国栋] 南华大学;[李杰] 永州职业技术学院

关键词： 幽门螺杆菌;Tipα蛋白;IL-6/STAT3信号通路;胃癌;增殖;迁移

摘要： 目的 表达并纯化重组幽门螺杆菌(Helicobacter pylori,Hp)肿瘤坏死因子-α诱导蛋白(tumor necrosis factor-α-inducing protein,Tipα),观察其对胃癌细胞增殖和迁移的影响以及IL-6/STAT3信号通路在其中的作用. 方法 采用IPTG诱导表达Tipα重组蛋白并通过Ni2+亲和层析纯化,纯化产物进行SDS-PAGE和Western blot鉴定;使用重组Tipα蛋白刺激胃癌SGC7901细胞,分别采用CCK8、流式细胞术、划痕伤口愈合试验和Transwell试验检测其对细胞增殖、凋亡和迁移的影响. 结果 可溶性25×10 3重组Tipα蛋白被成功诱导表达且能被抗His-tag单克隆抗体所识别.与空白对照组相比,Tipα蛋白能够抑制SGC7901细胞凋亡(t=9.53;P＜0.05).与PBS组相比,Tipα组细胞的增殖和迁移能力增加,而IL-6单克隆抗体或STAT3抑制剂SH-4-54预处理能够减弱Tipα诱导的细胞增殖和迁移(CCK8实验,F=103.194,P＜0.01;划痕伤口愈合试验,F=15.568,P＜0.01;Transwell试验,F=36.017,P＜0.01). 结论 Hp Tipα蛋白可促进胃癌细胞增殖和迁移,IL-6/STAT3信号通路在其中发挥重要作用.

语种： 中文

作者： Tang, Yunlian;Zhong, Yating;Fu, Ting;Zhang, Yang;Cheng, Allan;...

期刊： Biomedicine & Pharmacotherapy,2019年116:108984 ISSN：0753-3322

通讯作者： Gan, Runliang

作者机构： [Gan, Runliang; Zhang, Yang; Dai, Yongming; Cheng, Allan; Qu, Jiani; Zhong, Yating; Tang, Yunlian; Fu, Ting] Univ South China, Med Coll Hengyang, Canc Res Inst, Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Peoples R China.;[Zhong, Yating] First Peoples Hosp Changde City, Dept Pathol, Changde 415000, Hunan, Peoples R China.

通讯机构： [Gan, Runliang] U;Univ South China, Med Coll Hengyang, Canc Res Inst, Key Lab Tumor Cellular & Mol Pathol, Hengyang 421001, Peoples R China.

关键词： EB virus (EBV);Lymphocyte transformation;Lymphoblast;Gene expression profile;Key gene

摘要： Although the Epstein-Barr virus (EBV) is a well-known human oncogenic virus, its molecular mechanisms involved in the transformation of healthy human cells remain poorly understood. In this study, human lymphocytes were isolated from the peripheral blood of healthy adults, and lymphocytes were transformed in vitro by EBV. Agilent human whole genome microarrays were used to detect the differential gene expression profiles of EBV-transformed lymphoblasts and healthy peripheral blood lymphocytes (PBLs). By constructing the gene functional network of EBV-induced lymphocyte transformation, we screened out candidate key genes in this process and verified their expression levels by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. In the EBV-transformed lymphoblasts, 2335 differentially expressed genes, including 1328 up-regulated and 1007 down-regulated, were screened out. Five candidate key genes, namely, PLK1, E2F1, PTPN11, BIRC5 and FYN were mainly screened out according to the results of LIMMA, String, Cytoscape software analysis. RT-qPCR and Western blot showed that PLK1, E2F1, PTPN11, BIRC5 genes had increased expression levels, and FYN gene was down-regulated in EBV-transformed lymphoblasts. Silencing of PLK1 gene in Raji cells could inhibit cell proliferation and invasion, and induce cell cycle arrest and apoptosis. In conclusion, PLK1, E2F1, PTPN11, BIRC5 and FYN are the candidate key molecules of EBV-transformed lymphocytes. © 2019

语种： 英文

作者： Yajun Chenx;Xianpeng Dai;Yao Yao;Jing Wang;Xinzhi Yang;...

期刊： 生物化学与生物物理学报,2019年51(3):335-337 ISSN：1672-9145

通讯作者： Zhong, J.

作者机构： [Yao Yao; Zhong J.; Yang X.; Wang J.] Institute of Clinical Medicine, First Affiliated Hospital of University of South China, Hengyang, 421001, China;Department of Metabolism and Endocrinology, Second Affiliated Hospital of University of South China, Hengyang, 421001, China;[Dai X.] Department of General Surgery, Second Affiliated Hospital of University of South China, Hengyang, 421001, China;[Zhang Y.] Institute of Clinical Medicine, Second Affiliated Hospital of University of South China, Hengyang, 421001, China;Department of Metabolism and Endocrinology, First Affiliated Hospital of University of South China, Hengyang, 421001, China

通讯机构： [Zhong, J.] I;Institute of Clinical Medicine, First Affiliated Hospital of University of South China, Hengyang, China

关键词： MCF-7;apoptosis;expression;treatment;CYCLIN;can;Akt;its

摘要： Autophagy as a novel therapeutic target can inhibit or increase treatment efficacy in various types of breast cancer in a cell-type-dependent manner [1,2].Several studies have revealed that the coordination between Akt and the glycolytic pathway plays an indispensable role in mediating autophagy and caspase-dependent apoptosis,suggesting that a new regulatory mechanism for the process [3,4].Protein arginine N-methyltransferases(PRMTs)are eukaryotic enzymes that catalyze the transfer of methyl groups from S-adenosylmethionine to arginine residues of numerous PRMT substrates [5,6].PRMT2(also known as HRMT1L1)belongs to the arginine methyltransferase family [7].PRMT2β is a novel PRMT2 splice variant isolated from breast cancer cell [8].It occurs at the 3′ end of the PRMT2,resulting in loss of exons 7–9 and downstream frame-shifting [9].PRMT2β possesses 83 new amino acids at the C-terminus and its size is 301 amino acids.Our previous study reported that PRMT2β has potential antitumor effect by suppressing cyclin D1 expression [10].However,little is known about whether PRMT2β could regulate autophagy and glycolysis of MCF-7 cells.

语种： 英文

作者： 韩亮;陈国栋;曾莎莎;肖玲巧;张洁雅;...

期刊： 免疫学杂志,2019年35(2):105-111 ISSN：1000-8861

作者机构： 南华大学衡阳医学院病原生物学研究所,衡阳,421001

关键词： 幽门螺杆菌;IL-6/STAT3信号通路;胃癌

摘要： 目的研究IL-6/STAT3信号通路在幽门螺杆菌Tipα诱导胃癌细胞EMT形成中的作用。方法使用Tipα刺激胃癌SGC7901细胞,显微镜观察细胞形态变化;Western blot及qRT-PCR检测EMT标志物E-cadherin、N-cadherin和Vimentin的表达;免疫荧光检测STAT3的定位情况;ELISA检测IL-6水平;Western blot分析Tipα对SGC7901细胞p-STAT3和STAT3表达的影响。进一步应用IL-6中和抗体和STAT3抑制剂SH-4-54预处理细胞,以阻断IL-6/STAT3信号通路,继而检测通路阻断后对Tipα诱导的EMT形成的影响。结果 Tipα刺激SGC7901细胞12 h后细胞明显延长并出现伪足,EMT标志物N-cadherin和Vimentin表达增高,而E-cadherin表达减少。Tipα刺激能够增加IL-6分泌水平,且在6 h达峰值。Tipα可以浓度和时间依赖性方式诱导细胞p-STAT3水平增高。IL-6中和抗体及STAT3抑制剂预处理可逆转Tipα诱导的EMT形成。结论幽门螺杆菌Tipα可通过激活IL-6/STAT3信号通路诱导胃癌细胞EMT的形成。

语种： 中文

作者： Tan, Yuan;Li, Yumeng;Zhang, Yang;Yu, Jian;Wen, Yating;...

期刊： Immunologic Research,2018年66(4):471-479 ISSN：0257-277X

通讯作者： Wu, Yimou

作者机构： [Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Yu, Jian; Lu, Chunxue; Wu, Yimou; Li, Yumeng; Tan, Yuan; Wen, Yating; Wang, Chuan; Xu, Man; Chen, Qian] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.;[Tan, Yuan] First Hosp Changsha City, Dermatol, Changsha 410000, Hunan, Peoples R China.;[Zhang, Yang] Univ South China, Dept Pathol, Hengyang 421001, Peoples R China.

通讯机构： [Wu, Yimou] U;Univ South China, Inst Pathogen Biol, Med Coll, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

关键词： *C. psittaci;*CD4+ T cells;*CPSIT_p7;*Plasmid protein;*Protective immunity

摘要： The present study evaluated the immune-protective efficacy of the Chlamydia psittaci (C. psittaci) plasmid protein CPSIT_p7 and analyzed the potential mechanisms of this protection. The current study used recombinant CPSIT_p7 protein with Freund’s complete adjuvant and Freund’s incomplete adjuvant to vaccinate BALB/c mice. Adjuvants alone or PBS formulated with the same adjuvants was used as negative controls. Mice were intranasally challenged with 105 inclusion-forming units (IFU) of C. psittaci. We found that CPSIT_p7 vaccination significantly decreased the mouse lung chlamydial load, interferon-γ (IFN-γ) level, and pathological injury. This protection correlated well with specific humoral and cellular immune responses against C. psittaci. In vitro or in vivo neutralization of C. psittaci with sera harvested from immunized mice did not reduce the number of recoverable C. psittaci in the infected lungs, but CD4+ spleen cells collected from CPSIT_p7-immunized mice significantly decreased the chlamydial load via adoptive transfer to native mice. These results reveal that the protection conferred by CPSIT_p7 is dependent on CD4+ T cells. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

语种： 英文

作者： Ding, Chengming;He, Jun;Zhao, Jun;Li, Junhua;Chen, Jie;...

期刊： Cell Proliferation,2018年51(5):e12464- ISSN：0960-7722

通讯作者： Peng, Jian;Xia, Zanxian

作者机构： [Jia, Zeming; Chen, Jie; Ding, Chengming; Zhang, Yaoting; Peng, Jian] Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha, Hunan, Peoples R China.;[He, Jun; Li, Chong; Ding, Chengming; Zhu, Zhu; Han, Dong] Univ South China, Dept Hepatopancreatobiliary Surg, Affiliated Hosp 1, Hengyang, Peoples R China.;[Li, Junhua; Ding, Chengming; Zhao, Jun] Univ Southern Calif, Dept Mol Microbiol & Immunol, Norris Comprehens Canc Ctr, Los Angeles, CA USA.;[Liao, Wenyan] Univ South China, Dept Obstet & Gynecol, Affiliated Hosp 1, Hengyang, Peoples R China.;[Zeng, Yi] Youjiang Med Univ Nationalities, Dept Pathol & Immunol, Baise, Peoples R China.

通讯机构： [Peng, Jian; Xia, Zanxian] C;Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha, Hunan, Peoples R China.;Cent S Univ, State Key Lab Med Genet, Changsha, Hunan, Peoples R China.;Cent S Univ, Sch Life Sci, Changsha, Hunan, Peoples R China.

关键词： colorectal cancer;innate immunity;IRF3;RIG-I-like receptor (RLR)-mediated IFN-β signalling pathway;β-catenin

摘要： Objective: β-catenin is one of the most critical oncogenes associated with many kinds of human cancers, especially in the human CRC. Innate immunity recognizes tumour derived damage-associated molecular patterns (DAMPs) and primes the anti-tumour adaptive responses. While the function of β-catenin in CRC tumourigenesis is well established, its impact on innate immune evasion is largely unknown. The aim of this study is to characterize the role of β-catenin in inhibiting RIG-I-like receptor (RLR)-mediated IFN-β signalling in colorectal cancer. Materials and Methods: Immunohistochemical staining and western blotting were conducted to study the expression of β-catenin, IRF3 and phospho-IRF3 (p-IRF3) in CRC samples and cell lines. Plaque assay determining virus replication was performed to assess the regulation of β-catenin on IFN-β signalling. The inhibition of β-catenin on RLR-mediated IFN-β signalling was further studied by real-time analyses and reporter assays in the context of lentiviral-mediated β-catenin stably knocking down. Lastly, co-immunoprecipitation and nuclear fractionation assay were conducted to monitor the interaction between β-catenin and IRF3. Results: We found that high expression of β-catenin positively correlated with the expression of IRF3 in CRC cells. Overexpression of β-catenin increased the viral replication. Conversely knocking down of β-catenin inhibited viral replication. Furthermore, our data demonstrated that β-catenin could inhibit the expression of IFN-β and interferon-stimulated gene 56 (ISG56). Mechanistically, we found that β-catenin interacted with IRF3 and blocked its nuclear translocation. Conclusion: Our study reveals an unprecedented role of β-catenin in enabling innate immune evasion in CRC. © 2018 John Wiley & Sons Ltd

语种： 英文

作者： Zhang, Yunqiang;Tu, Lijun;Zhou, Xiuhong;Li, Bin

期刊： Medical science monitor basic research,2018年24:216-224 ISSN：2325-4394

作者机构： [Li, Bin; Zhang, Yunqiang] Department of Neurosurgery, Chenzhou No. 1 People's Hospital, Chenzhou, Hunan, China (mainland);[Tu, Lijun] Electrocardiographic Room, Chenzhou No. 1 People's Hospital, Chenzhou, Hunan, China (mainland);[Zhou, Xiuhong] Department of Pathophysiology, University of South China, Hengyang, Hunan, China (mainland)

摘要： BACKGROUND Curcumin has clear anti-tumor activity in various carcinomas. It regulates various signaling pathways like Wnt/β-catenin and JAK2/STAT3, which play vital roles in cell proliferation of several carcinomas, but to the best of our knowledge, there are currently no published reports on human glioma CHME cells. Therefore, the aim of this study was to explore the effect of curcumin on human glioma CHME cells. MATERIAL AND METHODS The CHME cell line was purchased from American Type Culture Collection (ATCC). The expressions of caspases 3, caspases 9, PARP, BAX, and BCL2 were detected by Western blot. Annexin V FITC, mitochondrial membrane potential, and reactive oxygen species were detected by flow cytometry. DAPI staining was detected by fluorescence microscopy. Cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. RESULTS We found that curcumin has cytotoxic activity in human glioma CHME cells, as shown by DAPI staining, annexin V/PI, and nuclear morphology. We found that cell growth decreased with increased concentration of curcumin, as well as sowing effects on expression of caspase-3, caspase-9, and cleavage of PARP, which suggests apoptotic cascade activity. The increase in reactive oxygen species and loss of mitochondrial membrane potential (Δψmt) in concentration-dependent manners suggests biochemical induction of apoptosis in CHME cells. CONCLUSIONS Curcumin has effective anticancer activity in human glioma CHME cells by inducing the apoptotic pathway.

语种： 英文

作者： Zhang, Yunsheng;Li, Fang;Liu, Luogen;Jiang, Hongtao;Jiang, Xiaorong;...

期刊： Life Sciences,2018年207:451-460 ISSN：0024-3205

通讯作者： Wang, Yi

作者机构： [Wang, Yi; Zhang, Yunsheng; Ge, Xin; Liu, Luogen] Univ South China, Affiliated Hosp 2, Clin Res Inst, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.;[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.;[Jiang, Hongtao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang 421001, Peoples R China.;[Wang, Zhenggen; Zhang, Li; Jiang, Xiaorong] Univ South China, Affiliated Hosp 2, Dept Gastroenterol, Hengyang 421001, Peoples R China.;[Cao, Jingsong] Univ South China, Med Coll, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

通讯机构： [Wang, Yi] U;Univ South China, Affiliated Hosp 2, Clin Res Inst, 35 Jiefang Ave, Hengyang 421001, Hunan, Peoples R China.

关键词： ATG3/AKT/mTOR signaling axis;Autophagy;PC-3 cells;Salinomycin

摘要： Aims: This study evaluated the mechanism by which salinomycin-induced autophagy blocks apoptosis in PC-3 prostate cancer cells. Main methods: The anti-cancer effects of salinomycin in PC-3 cells were confirmed by flow cytometry, JC-1 staining and western blotting. Then, the autophagic effects were measured by western blotting, GFP-LC3 puncta formation assay, immunofluorescence staining and electron microscopy. Furthermore, we used lentivirus-mediated shRNA to silence ATG3, ATG5 and ATG7 expression in PC-3 cells to investigate the regulatory mechanisms of salinomycin-induced autophagy. Key findings: Salinomycin could induce apoptosis and autophagy in PC-3 cells. Interestingly, autophagy inhibition could enhance salinomycin-induced apoptosis. We further showed that ATG3, a known critical regulator of autophagy, was downregulated and involved in the inhibition of apoptosis by salinomycin-induced autophagy via the AKT/mTOR signaling axis. Significance: Our data indicated that salinomycin-induced autophagy blocks apoptosis via the ATG3/AKT/mTOR signaling axis in PC-3 cells, which provides new clues for the mechanisms of underlying the anti-cancer effects of salinomycin. © 2018

语种： 英文

作者： Cao, Jingsong;Liu, Luogen;Zhang, Yunsheng;Xiao, Jianhua*;Wang, Yi*

期刊： BMC Immunology,2017年18(1):1-8 ISSN：1471-2172

通讯作者： Xiao, Jianhua;Wang, Yi

作者机构： [Xiao, Jianhua; Cao, Jingsong] Univ South China, Med Coll, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Jianhua; Cao, Jingsong] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;[Wang, Yi; Zhang, Yunsheng; Xiao, JH; Wang, Y; Xiao, Jianhua; Liu, Luogen; Cao, Jingsong] Univ South China, Affiliated Hosp 2, Inst Pathogen Biol, Clin Res Ctr,Med Coll, Hengyang 421001, Hunan, Peoples R China.;[Wang, Yi] Univ South China, Affiliated Hosp 2, Urinary Surg, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Xiao, Jianhua; Xiao, JH; Wang, Y] U;Univ South China, Med Coll, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 2, Inst Pathogen Biol, Clin Res Ctr,Med Coll, Hengyang 421001, Hunan, Peoples R China.

关键词： Blood group B antigen;Blood group B antibody;HK2;B cells activation;ABOi-KT

摘要： Background: It is well known that ABO blood group system incompatible kidney transplantation (ABOi-KT) is an effective strategy for end-stage renal disease. The main barrier for ABOi-KT is how to keep host B cell activation and blood group antibody titer in low levels. Moreover, the mechanism of B cell activation induced by blood group antigen was unclear in ABOi-KT. Results: In this study, HK2 cells were identified to express blood group B antigen when cocultured with lymphocytes of blood group A. Optical microscope observation demonstrated that HK2 cells in coculture group gradually decreased. Furthermore, flow cytometer assay identified that T cell phenotypes (CD3+, CD3+CD4+ and CD3+CD8+) had no significant change and B cell phenotypes (CD19+ and CD138+) were all significantly enhanced (3.07 and 3.02 folds) at day 4. In addition, immunoturbidimetry analysis demonstrated that blood group B antibody was significantly increased to 2.35 fold at day 4, IgG was significantly increased to 3.60 and 2.81 folds at days 4 and 8 respectively, while IgM had no significant change at the measured time points. Conclusions: Taken together, B cells were activated and secreted blood group B antibody after treatment with HK2 expressing blood group B antigen. The results of this study maybe useful for further determination of the mechanism of B cell activation after ABO incompatible kidney endothelial cells stimulation. © 2017 The Author(s).

语种： 英文

作者： Deng, Hongli;Li, Zhibo;Liu, Guang;Li, Xianhua;Chen, Yong;...

期刊： Gynecological Endocrinology,2017年33(5):363-367 ISSN：0951-3590

通讯作者： Zhang, Yong;Sun, Yifan;Fu, Jinjian

作者机构： [Deng, Hongli; Liu, Guang; Li, Zhibo; Li, Xianhua] Univ South China, Peoples Hosp Liuyang City, Dept Clin Lab, Affiliated Liuyang Hosp, Changsha, Hunan, Peoples R China.;[Chen, Yong] Univ South China, Dept Clin Lab, Affiliated Changsha Hosp, Hosp Changsha City 1, Changsha, Hunan, Peoples R China.;[Zhang, Yong] Univ South China, Dept Gastrointestinal Surg, Affiliated Liuyang Hosp, Peoples Hosp Liuyang City, Renmin Rd 119, Changsha 410300, Hunan, Peoples R China.;[Sun, Yifan] Guangxi Univ Chinese Med, Dept Clin Lab, Affiliated Hosp 3, 32 Jiefang North Rd, Liuzhou 545001, Guangxi, Peoples R China.;[Fu, Jinjian] Liuzhou Matern & Child Hlth Care Hosp, Dept Clin Lab, 50th Yingshan Rd, Liuzhou 545001, Guangxi, Peoples R China.

通讯机构： [Zhang, Yong] U;[Sun, Yifan] G;[Fu, Jinjian] L;Univ South China, Dept Gastrointestinal Surg, Affiliated Liuyang Hosp, Peoples Hosp Liuyang City, Renmin Rd 119, Changsha 410300, Hunan, Peoples R China.;Guangxi Univ Chinese Med, Dept Clin Lab, Affiliated Hosp 3, 32 Jiefang North Rd, Liuzhou 545001, Guangxi, Peoples R China.

关键词： Biomarker;inflammatory;insulin resistance;interferon gamma-induced protein 10;polycystic ovary syndrome

摘要： Background: Interferon γ-induced protein 10 kDa (IP10/CXCL10) is a chemokine related to endocrine disorders; however, the serum concentrations of IP10 in women with polycystic ovary syndrome (PCOS) have not yet been reported. Therefore, we investigated whether IP10 is increased in PCOS patients and its potential clinical value in PCOS patients. Methods: For this research, the serum IP10, glucose, insulin, high sensitivity C-reactive protein (hs-CRP), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and total testosterone (TT) concentrations were measured in 60 women with PCOS and healthy controls. Results: The median IP10 concentration was 45.60 pg/mL [interquartile range (IQR):29.75, 79.69], which was significantly higher than that of the body mass index (BMI)-matched controls (median: 36.46 pg/mL; IQR:28.98, 45.80). In the multivariate linear regression analysis, hs-CRP and the homeostasis model assessment of insulin resistance index (HOMA2-IR) were independent predictors of the IP10 values, while FSH was inversely associated with the IP10.No significant association was observed between the IP10 and BMI, glucose, LH and TT. Conclusions: The serum IP10 concentrations increase in women with PCOS, moreover, IP10 appears to be correlated with the inflammatory and IR statuses of PCOS. IP10 may be a potential biomarker to estimate the disease activity of PCOS. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： Zhang, Yunsheng;Liu, Luogen;Li, Fang;Wu, Tao;Jiang, Hongtao;...

期刊： BioMed Research International,2017年2017:4101653 ISSN：2314-6133

通讯作者： Wang, Yi

作者机构： [Wang, Yi; Zhang, Yunsheng; Liu, Luogen] Univ South China, Affiliated Hosp 2, Clin Res Inst, Hengyang 421001, Peoples R China.;[Li, Fang] Hunan Polytech Environm & Biol, Coll Nursing, Hengyang 421005, Peoples R China.;[Wang, Yi; Wu, Tao; Jiang, Xianxun; Jiang, Hongtao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang 421001, Peoples R China.;[Du, Xiaobo] First Peoples Hosp Yueyang, Dept Urol, Yueyang 414000, Peoples R China.

通讯机构： [Wang, Yi] U;Univ South China, Affiliated Hosp 2, Clin Res Inst, Hengyang 421001, Peoples R China.

摘要： Salinomycin is an antibiotic isolated from Streptomyces albus that selectively kills cancer stem cells (CSCs). However, the antitumor mechanism of salinomycin is unclear. This study investigated the chemotherapeutic efficacy of salinomycin in human prostate cancer PC-3 cells. We found that cytotoxicity of salinomycin to PC-3 cells was stronger than to nonmalignant prostate cell RWPE-1, and exposure to salinomycin induced G2/M phage arrest and apoptosis of PC-3 cells. A mechanistic study found salinomycin suppressed Wnt/β-catenin pathway to induce apoptosis of PC-3 cells. An in vivo experiment confirmed that salinomycin suppressed tumorigenesis in a NOD/SCID mice xenograft model generated from implanted PC-3 cells by inhibiting the Wnt/β-catenin pathway, since the total β-catenin protein level was reduced and the downstream target c-Myc level was significantly downregulated. We also showed that salinomycin, but not paclitaxel, triggered more apoptosis in aldehyde dehydrogenase- (ALDH-) positive PC-3 cells, which were considered as the prostate cancer stem cells, suggesting that salinomycin may be a promising chemotherapeutic to target CSCs. In conclusion, this study suggests that salinomycin reduces resistance and relapse of prostate tumor by killing cancer cells as well as CSCs. © 2017 Yunsheng Zhang et al.

语种： 英文

作者： Chen, Guodong;Tang, Na;Wang, Chao;Xiao, Linqiao;Yu, Minjun;...

期刊： Biochemical and Biophysical Research Communications,2017年484(2):311-317 ISSN：0006-291X

通讯作者： Zhang, Yan

作者机构： [Han, Liang; Wang, Chao; Chen, Guodong; Tang, Na; Zhao, Lanhua; Cai, Hengling; Xie, Chengyuan; Yu, Minjun; Zhang, Yan; Xiao, Linqiao] Univ South China, Coll Med, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

通讯机构： [Zhang, Yan] U;Univ South China, Coll Med, Inst Pathogen Biol, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： Helicobacter pylori;TNF-alpha inducing protein (Tip alpha);IL-6/STAT3 pathway;Gastric cancer;Epithelial-mesenchymal transition (EMT)

摘要： Tumor necrosis factor (TNF)-α-inducing protein (Tipα) is a newly identified carcinogenic factor secreted by Helicobacter pylori (H. pylori). Although it has been proved that Tipα is a strong inducer of epithelial-mesenchymal transition (EMT), a crucial process of migration, the exact molecular mechanism is unknown. Current evidence indicates that the oncogenic transcription factor signal transducers and activators of transcription 3 (STAT3) is inappropriately activated in multiple malignancies, including gastric cancer. In this study, we showed that Tipα significantly down-regulated the expression of EMT-related markers E-cadherin as well as up-regulated N-cadherin and vimentin in SGC7901 cells, with typical morphological changes of EMT. Tipα also promoted proliferation and migration of SGC7901 cells. Furthermore, Tipα activated interleukin-6 (IL-6)/STAT3 signaling pathway in SGC7901 cells. The effects of Tipα treatment observed was abolished when we block IL-6/STAT3 signaling pathway. Altogether, our data demonstrated that Tipα may accelerate tumor aggressiveness in gastric cancer by promoting EMT through activation of IL-6/STAT3 pathway. © 2017 Elsevier Inc.

语种： 英文

作者： Li, Bi-Rong;Xia, Lin-Qin;Liu, Jing;Liao, Lin-Ling;Zhang, Yang;...

期刊： Biochemical and Biophysical Research Communications,2017年494(1-2):384-389 ISSN：0006-291X

通讯作者： He, Ping-Ping;Ouyang, Xin-Ping

作者机构： [Zhang, Yang; Deng, Min; Li, Bi-Rong] Shaoyang Med Coll, Dept Physiol, Shaoyang 422000, Hunan, Peoples R China.;[He, Ping-Ping; Ouyang, Xin-Ping] Univ South China, Med Coll, Neurosci Inst, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;[Xia, Lin-Qin] Shaoyang Med Coll, Dept Pathol, Shaoyang 422000, Hunan, Peoples R China.;[Liu, Jing] Shaoyang Med Coll, Fac Nursing, Shaoyang 422000, Hunan, Peoples R China.;[Liao, Lin-Ling] Shaoyang Med Coll, Affiliated Hosp, Dept Hematol Oncol, Shaoyang 422000, Hunan, Peoples R China.

通讯机构： [He, Ping-Ping; Ouyang, Xin-Ping] U;Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Med Coll, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.

关键词： *Atherosclerosis;*CD36;*Cholesterol uptake;*miR-758-5p

摘要： miR-758-3p plays an important role via regulting ABCA1-mediated cholesterol efflux in atherosclerosis. However, the mechanism of miR-758-5p in cholesterol metabolism is still unclear. Here, we revealed that miR-758-5p decreased total cholesterol accumulation in THP-1 macrophage derived foam cells through markedly reducing cholesterol uptake, and no effect on the cholesterol efflux. Interestingly, computational analysis suggests that CD36 may be a target gene of miR-758-5p. Our study further demonstrated that miR-758-5p decreased CD36 expression at both protein and mRNA levels via targeting the CD36 3′UTR in THP-1 macrophage derived foam cells. The present present study concluded that miR-758-5p decreases lipid accumulation of foam cell via regulating CD36-mediated the cholesterol uptake. Therefore, targeting miR-758-5p may offer a promising strategy to treat atherosclerotic vascular disease. © 2017

语种： 英文

作者： Liu, Mi-Hua;Shan, Jian;Li, Jian;Zhang, Yuan;Lin, Xiao-Long*

期刊： EXPERIMENTAL AND THERAPEUTIC MEDICINE,2016年12(2):1113-1118 ISSN：1792-0981

通讯作者： Lin, Xiao-Long

作者机构： [Liu, Mi-Hua] Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, Hengyang 421001, Hunan, Peoples R China.;[Shan, Jian] Zhongshan Torch Dev Zone Hosp, Dept Pathol, Zhongshan 528437, Guangdong, Peoples R China.;[Li, Jian] BoAi Hosp Zhongshan, Dept Ultrason Diag, Zhongshan 528403, Guangdong, Peoples R China.;[Zhang, Yuan] Mawangdui Hosp, Dept Pathol, Changsha 410016, Hunan, Peoples R China.;[Lin, Xiao-Long] Guangzhou Med Univ, Affiliated Huizhou Hosp, Peoples Hosp Huizhou 3, Dept Pathol, 1 Xuebei St, Huizhou 516002, Guangdong, Peoples R China.

通讯机构： [Lin, Xiao-Long] G;Guangzhou Med Univ, Affiliated Huizhou Hosp, Peoples Hosp Huizhou 3, Dept Pathol, 1 Xuebei St, Huizhou 516002, Guangdong, Peoples R China.

关键词： apoptosis;doxorubicin;forkhead box protein O1;resveratrol;sirtuin 1

摘要： Doxorubicin (DOX) is an efficient drug used in cancer therapy; however, it can induce severe cytotoxicity, which limits its clinical application. In the present study, the effects of resveratrol (RES) on sirtuin 1 (SIRT1) activation in mediating DOX-induced cytotoxicity in H9c2 cardiac cells was investigated. H9c2 cells were exposed to 5 μM DOX for 24 h to establish a model of DOX cardiotoxicity. Apoptosis of H9c2 cardiomyocytes was assessed using the MTT assay and Hoechst nuclear staining. The results demonstrated that pretreating H9c2 cells with RES prior to the exposure of DOX resulted in increased cell viability and a decreased quantity of apoptotic cells. Western blot analysis demonstrated that DOX decreased the expression level of SIRT1. These effects were significantly alleviated by co-treatment with RES. In addition, the results demonstrated that DOX administration amplified forkhead box O1 (FoxO1) and P53 expression levels in H9c2 cells. RES was also found to protect against DOX-induced increases of FoxO1 and P53 expression levels in H9c2 cells. Furthermore, the protective effects of RES were arrested by the SIRT1 inhibitor nicotinamide. In conclusion, the results demonstrated that RES protected H9c2 cells against DOX-induced injuries via SIRT1 activation. © 2016, Spandidos Publications. All rights reserved.

语种： 英文

作者： Liu, Mi-Hua*;Lin, Xiao-Long;Guo, Dong-Ming;Zhang, Yuan;Yuan, Cong;...

期刊： MOLECULAR MEDICINE REPORTS,2016年13(2):1281-1286 ISSN：1791-2997

通讯作者： Liu, Mi-Hua;He, Jun

作者机构： [Liu, MH; He, J; Tan, Tian-Ping; Wu, Shao-Jian; Liu, Mi-Hua; He, Jun; Chen, Yu-Dan; Ye, Zu-Feng] Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, 336 Dongfeng South Rd, Hengyang 421001, Hunan, Peoples R China.;[Lin, Xiao-Long] Guangzhou Med Univ, Peoples Hosp Huizhou 3, Dept Pathol, Huizhou 516002, Guangdong, Peoples R China.;[Guo, Dong-Ming] Univ South China, Lab Clin Anat, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Yuan] Mawangdui Hosp, Dept Pathol, Changsha 410016, Hunan, Peoples R China.;[Yuan, Cong] First Hosp Changsha, Dept Cardiol, Changsha 410005, Hunan, Peoples R China.

通讯机构： [Liu, MH; He, J] U;Univ South China, Affiliated Nanhua Hosp, Dept Clin Lab, 336 Dongfeng South Rd, Hengyang 421001, Hunan, Peoples R China.

关键词： resveratrol;doxorubicin;apoptosis;P53;adenosine monophosphate-activated protein kinase

摘要： 'Obesity Facts' publishes articles covering all aspects of obesity, in particular epidemiology, etiology and pathogenesis, treatment, and the prevention of adiposity. As obesity is related to many disease processes, the journal is also dedicated to all topics pertaining to comorbidity and covers psychological and sociocultural aspects as well as influences of nutrition and exercise on body weight. The editors carefully select papers to present only the most recent findings in clinical practice and research. All professionals concerned with obesity issues will find this journal a most valuable update to keep them abreast of the latest scientific developments.Special sections comprising a variety of subspecialties reinforce the journal's value as an exhaustive record of recent progress for all internists, gastroenterologists, endocrinologists, pediatricians, dieticians, nutritionists, bariatric surgeons, psychologists and psychiatrists, occupational health practitioners, sports medicine specialists, ecotrophologists, sociologists, and biologists as well as prevention and public health researchers. In addition, 'Obesity Facts' serves as an ideal information tool for the members of the pharmaceutical and food industry as well as those active in nutritional research and medicine.

语种： 英文