期刊论文

TNF - α - inducing protein of Helicobacter pylori induces epithelialmesenchymal transition (EMT) in gastric cancer cells through activating IL-6/STAT3 signaling pathway

中文

免疫学杂志

1000-8861

2019

35

2

105-111

10.13431/j.cnki.immunol.j.20190017

湖南省高校创新平台开放基金（16K078）； “特殊病原体防控”湖南省科技厅重点实验室资助项目【湘科计字（2014）5号】； 湖南省分子靶标新药研究协同创新中心资助项目【湘教通（2014）405号、（2015）351号】； 南华大学大学生研究性学习和创新性实验计划（2018XJXZ384）；

本校为第一机构

目的研究IL-6/STAT3信号通路在幽门螺杆菌Tipα诱导胃癌细胞EMT形成中的作用。方法使用Tipα刺激胃癌SGC7901细胞,显微镜观察细胞形态变化;Western blot及qRT-PCR检测EMT标志物E-cadherin、N-cadherin和Vimentin的表达;免疫荧光检测STAT3的定位情况;ELISA检测IL-6水平;Western blot分析Tipα对SGC7901细胞p-STAT3和STAT3表达的影响。进一步应用IL-6中和抗体和STAT3抑制剂SH-4-54预处理细胞,以阻断IL-6/STAT3信号通路,继而检测通路阻断后对Tipα诱导的EMT形成的影响。结果 Tipα刺激SGC7901细胞12 h后细胞明显延长并出现伪足,EMT标志物N-cadherin和Vimentin表达增高,而E-cadherin表达减少。Tipα...

The aim of this study was to evaluate the role of IL-6/STAT3 signaling pathway in epithelialmesenchymal transition(EMT) of gastric cancer cells induced by TNF-α-inducing protein(Tipα) of Helicobacter pylori(H. pylori). Tipα protein was purified and used to stimulate the human gastric cancer cell line SGC7901 cells. Then the morphological changes of SGC7901 cell were observed under a microscope, and the protein and mRNA levels of EMT-related molecules including E-cadherin, N-cadherin and Vimentin were detected by Western blotting and qRT-PCR....