挥发曲线法测定鹅不食草挥发油的研究
作者：
刘运美;何军;陈文;郭玉;宋秀道
期刊：
广州化工 ,2013年41(14):91-92 ISSN：1001-9677
作者机构：
南华大学药学与生物科学学院药学系,湖南衡阳,421001;南华大学化学化工学院,湖南衡阳,421001
关键词：
鹅不食草;挥发油;挥发曲线法;含量测定
摘要：
建立了鹅不食草挥发油含量测定新方法,并对水蒸汽蒸馏法一些提取条件（如粉末粗细,萃取溶剂,浸泡等）进行优化,以确定最佳提取方案。具体方法是：利用水蒸汽蒸馏法提取鹅不食草中挥发油,采用挥发曲线法测定挥发油含量。结果表明：水蒸汽蒸馏法的提取率最佳工艺是粉末先浸泡1～2 h,再进行水蒸气蒸馏法,用乙醚萃取为佳。挥发曲线法RSD为0.959%。挥发曲线法测定挥发油含量是一种成本低廉,操作简便,准确的测定方法。
语种：
中文
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宁乡花猪原代和第一代血液生理生化指标比较
作者：
丁婧璇;王宗保;肖国华;张素君;余坚
期刊：
中国比较医学杂志 ,2013年23(9):6-11 ISSN：1671-7856
作者机构：
南华大学药学与生物科学学院,衡阳,421001;[王宗保; 丁婧璇; 余坚; 张素君; 肖国华] 南华大学
关键词：
宁乡花猪;血液生理指标;血液生化指标
摘要：
目的 比较原代宁乡花猪与其第一代仔猪血液生理生化指标的变化.方法 常规方法测定宁乡花猪F0代16头,F1代16头血液18项生理指标和13项生化指标.结果 F0代与F1代部分血液生理指标如白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、红细胞积压(HCT)等13项有显著性差异(P＜0.05);部分血液生化指标如总胆红素(TBIL),尿素氮(BUN),总蛋白(TP),白/球蛋白(ALB/GLB),葡萄糖(GLU),有显著性差异(P＜0.05);其他指标均无统计学差异.结论 2代宁乡花猪之间部分血液生理生化指标有显著性差异.得到的数据可以为实验动物化研究提供相应的基础生理参考数据.
语种：
中文
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实验鱼类在药学研究中的应用
作者：
廖小立;姚峰;吴端生
期刊：
中国医药科学 ,2013年(22):18-21+26 ISSN：2095-0616
作者机构：
南华大学药学与生物科学学院实验动物学部,湖南衡阳,421001;[吴端生; 廖小立; 姚峰] 南华大学
关键词：
实验鱼类;药学;应用
摘要：
实验鱼类作为模式生物已经被广泛地应用于药学研究,其中犹以斑马鱼为经典。斑马鱼因其独特的优点,被建立人类重大疾病模型和药物筛选平台,取得了许多有价值的研究成果。另外,青鳉、虹鳟、剑尾鱼、稀有鮈鲫、红鲫、金鱼、鲤鱼等实验鱼类也具有在药物开发与应用研究的优势。本文综述了近年来国内外实验鱼类在药物毒理学与安全性评价、环境化合物安全性评价、药物筛选与新药发现、药物药理学研究、药物代谢研究、药物制剂学研究、中药研究、再生药物研究等药学研究的一些事例,为药学研究中实验动物的选择积累有价值的资料,并为实验鱼类应用研究提供新的启示。
语种：
中文
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Antagonism of Betulinic Acid on LPS-Mediated Inhibition of ABCA1 and Cholesterol Efflux through Inhibiting Nuclear Factor-kappaB Signaling Pathway and miR-33 Expression
作者：
Zhao, Guo-Jun;Tang, Shi-Lin;Lv, Yun-Cheng;Ouyang, Xin-Ping;He, Ping-Ping;...
期刊：
PLOS ONE ,2013年8(9):e74782- ISSN：1932-6203
通讯作者：
Yin, Kai
作者机构：
[Yin, Kai; He, Ping-Ping; Ouyang, Xin-Ping; Chen, Wu-Jun; Zhang, Min; Zhao, Guo-Jun; Lv, Yun-Cheng; Tang, Chao-Ke; Lu, Qian; Tang, Yan-Yan; Yao, Feng; Tang, Shi-Lin] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.;[Zhao, Guo-Jun] Univ South China, Dept Histol & Embryol, Hengyang, Hunan, Peoples R China.;[He, Ping-Ping] Univ South China, Sch Nursing, Hengyang, Hunan, Peoples R China.;[Fu, Yuchang] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA.;[Zhang, Da-Wei] Univ Alberta, Dept Pediat, Edmonton, AB, Canada.
通讯机构：
[Yin, Kai] U;Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang, Hunan, Peoples R China.
关键词：
Cholesterol;Transcription factors;Macrophages;Inflammation;Phosphorylation;Atherosclerosis;MicroRNAs;Inflammatory diseases
摘要：
ATP-binding cassette transporter A1 (ABCA1) is critical in exporting cholesterol from macrophages and plays a protective role in the development of atherosclerosis. The purpose of this study was to investigate the effects of betulinic acid (BA), a pentacyclic triterpenoid, on ABCA1 expression and cholesterol efflux, and to further determine the underlying mechanism. BA promoted ABCA1 expression and cholesterol efflux, decreased cellular cholesterol and cholesterol ester content in LPS-treated macrophages. Furthermore, we found that BA promoted ABCA1 expression via down-regulation of miR-33s. The inhibition of LPS-induced NF-kappa B activation further decreased miR-33s expression and enhanced ABCA1 expression and cholesterol efflux when compared with BA only treatment. In addition, BA suppressed I kappa B phosphorylation, p65 phosphorylation and nuclear translocation, and the transcription of NF-kappa B-dependent related gene. Moreover, BA reduced atherosclerotic lesion size, miR-33s levels and NF-kappa B activation, and promoted ABCA1 expression in apoE(-/-) mice. Taken together, these results reveal a novel mechanism for the BA-mediated ABCA1 expression, which may provide new insights for developing strategies for modulating vascular inflammation and atherosclerosis.
语种：
英文
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受体组分蛋白(RCP)在静压力诱导血管平滑肌细胞增殖及跨膜信号转导中的作用
作者：
郭锋;刘彦梅;陈根;全海燕;江高峰;...
期刊：
中国药理通讯 ,2013年30(03):55-55
作者机构：
[郭锋; 刘彦梅; 陈根; 全海燕; 江高峰; 秦旭平] 南华大学药物药理研究所血管生物学研究室
会议名称：
第十三届全国生化与分子药理学学术会议
会议时间：
2013-08-03
会议地点：
大庆
会议论文集名称：
第十三届全国生化与分子药理学学术会议论文集
关键词：
血管平滑肌细胞增殖;跨膜信号转导;压力诱导;组分蛋白;受体;降钙素基因相关肽;鼠源性
摘要：
目的:探讨降钙素基因相关肽受体组分蛋白(RCP)在静压力诱导血管平滑肌细胞(VSMC)增殖及跨膜信号转导中的作用.方法:以鼠源性A10血管平滑肌细胞(A10VSMC)作为研究对象,用不同静态压力(0,80,100,120,140,160,180 mmHg)在不同时间(0,3,6,12,24 h)处理血管平滑肌细胞.噻唑蓝比色法(MTT)检测细胞的活性;Western Blot 检测细胞增殖核原(PCNA)和RCP的蛋白表达;RT-PCR检测RCP mRNA表达水平.分别用小RNA干扰技术(siRNA)或CGRP沉默或诱导RCP基因的表达,免疫共沉淀(Co-IP)和Western Blot检测Caveolin-1/RCP蛋白是否存在相互作用.
语种：
中文
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CpG adjuvant enhances the mucosal immunogenicity and efficacy of a Treponema pallidum DNA vaccine in rabbits
作者：
Zhao, Feijun;Liu, Shuangquan;Zhang, Xiaohong;Yu, Jian;Zeng, Tiebing;...
期刊：
Human Vaccines & Immunotherapeutics ,2013年9(4):753-760 ISSN：2164-5515
通讯作者：
Wu, Yimou
作者机构：
[Wu, Yimou; Zhao, Feijun; Zeng, Tiebing] Univ South China, Pathogen Biol Inst, Hengyang City, Hunan, Peoples R China.;Centers Dis Control Hunan Prov, Changsha, Hunan, Peoples R China.;[Liu, Shuangquan] Cent S Univ, Dept Anat & Neurobiol, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Liu, Shuangquan; Cao, Xunyu] Univ South China, Affiliated Hosp 1, Hengyang, Hunan, Peoples R China.;[Zhang, Xiaohong] Univ South China, Dept Histol & Embryol, Sch Med, Hengyang, Hunan, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Pathogen Biol Inst, Hengyang City, Hunan, Peoples R China.
关键词：
DNA vaccine;CpG ODN;membrane protein;Treponema pallidum;Tp Gpd;mucosal adjuvant;mucosal immune
摘要：
Objectives: The protective response against Treponema pallidum (Tp) infection of a DNA vaccine enhanced by an adjuvant CpG ODN was investigated. Results: The mucosal adjuvant CpG ODN enhanced the production of higher levels of anti-TpGpd antibodies induced by pcD/Gpd-IL-2 in rabbits. It also resulted in higher levels of secretion of IL-2 and IFN-α, and facilitated T cell proliferation and differentiation (p < 0.05). No significant difference about testing index above-mentioned was found in the intranasal immunization group of pcD/Gpd-IL-2 vaccine adjuvanted by CpG ODN when compared with the immunization by pcD/ Gpd-IL-2 vaccine intramuscular injection alone (p > 0.05). Furthermore, CpG ODN stimulated the production of mucosaspecific anti-sIgA antibodies and resulted in the lowest Tp-positive rate (6.7%) for Tp-infection of skin lesions and the lowest rates (8.3%) of ulceration lesions, thus achieving better protective effects. Methods: New Zealand rabbits were immunized with the eukaryotic vector encoding recombinant pcD/Gpd-IL-2 using intramuscular multi-injection or together with mucosal enhancement via a nasal route. The effect of the mucosal adjuvant CpG ODN was examined. Conclusions: The CpG ODN adjuvant significantly enhances the humoral and cellular immune effects of the immunization by pcD/Gpd-IL-2 with mucosal enhancement via nasal route. It also stimulates strong mucosal immune effects, thus initiating more efficient immune-protective effects. © 2013 Landes Bioscience.
语种：
英文
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高龄化社会退休人力的社会参与分析与活化策略建构:以台湾社会的经验为例
作者：
魏书娥
作者机构：
[魏书娥] 南华大学生死学系
会议名称：
第三届"海峡两岸农村社会保险理论与实践"学术研讨会
会议时间：
2013-01-01
会议地点：
中国江苏南京
会议论文集名称：
海峡两岸农村社会保险理论与实践研究论文集
关键词：
高龄化社会;活化老化;社会参与
摘要：
世界卫生组织提出"活化老化"是迈入晚期工业资本主义经济阶段的高龄化社会之人,化解老年退休危机的核心观点。在此观点之下,老年退休者重新建立与社会连结的落实方式则必须仰赖劳动参与以及社会参与,劳动参与方式牵涉高龄社会的劳动市场结构变动所冲击的老年经济安全,而社会参与方式牵涉资本主义社会的社会资本类型所影响老年生活质量,两者之间的选取对退休者而言,基于其时间、体力和意愿的限制,既相互排挤,又相辅相成,尚
语种：
中文
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Assessment of heavy metal contamination and bioaccumulation in soybean plants from mining and smelting areas of southern Hunan Province, China
作者：
Zhou, Hang;Zeng, Min;Zhou, Xin;Liao, Bo-Han* ;Liu, Jun;...
期刊：
Environmental Toxicology and Chemistry ,2013年32(12):2719-2727 ISSN：0730-7268
通讯作者：
Liao, Bo-Han
作者机构：
[Zhou, Hang] Hunan Agr Univ, Coll Biosci & Technol, Changsha, Hunan, Peoples R China.;[Zhong, Qian-Yun; Liao, Bo-Han; Zeng, Hui; Zeng, Min; Zhou, Xin; Lei, Ming; Zhou, Hang] Cent South Univ Forestry & Technol, Inst Environm Sci & Engn, Changsha, Hunan, Peoples R China.;[Liu, Jun] Univ South China, Coll Pharm & Life Sci, Hengyang, Peoples R China.
通讯机构：
[Liao, Bo-Han] C;Cent South Univ Forestry & Technol, Inst Environm Sci & Engn, Changsha, Hunan, Peoples R China.
关键词：
Assessment;Geoaccumulation index;Heavy metal accumulation;Soil contamination;Soybean (Glycine max)
摘要：
Soybean is one of most important dicotyledonous food crops and is widely planted in Hunan Province, China. However, mining activity causes contamination of the soil in which soybean grows. To assess the impact of mining-induced soil contamination on soybean plants, a geoaccumulation index (I<inf>geo</inf>) was used to evaluate 20 soil samples from the mining and smelting areas of southern Hunan Province. The results indicated that Zn ranged from uncontaminated to a moderately contaminated level (I<inf>geo</inf><1), Pb was at a strongly contaminated level (I<inf>geo</inf>>3), and Cd was at an extremely contaminated level (I<inf>geo</inf>>5) across the whole study area. All of the studied soybean plants were affected by heavy metal Pb and Cd contamination, and the mean concentrations in seeds were 13.9mg/kg and 2.95mg/kg, respectively. The estimated bioconcentration factor and translocation factor showed that the soybean roots had a strong Cd bioconcentration capability and the stems had a strong translocation capability in terms of Pb, Cd, and Zn, with preferential transference of metals to the soybean leaves. The bioavailable fraction in the soil was characterized by the exchangeable fraction of heavy metals. In the present study, the bioavailable fractions of Pb, Cd, and Zn were significantly positively correlated with the concentration of these metals in soybean tissues (roots, stems, leaves, husks, and seeds). ©2013 SETAC.
语种：
英文
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Deuterium-depleted water (DDW) inhibits the proliferation and migration of nasopharyngeal carcinoma cells in vitro
作者：
Wang, Hongqiang;Zhu, Baohua;He, Zhiwei;Fu, Hui;Dai, Zhong;...
期刊：
Biomedicine & Pharmacotherapy ,2013年67(6):489-496 ISSN：0753-3322
通讯作者：
Fang, Weiyi
作者机构：
[He, Zhiwei; Wang, Hongqiang; Li, Binbin; Huang, Guoliang; Yang, Huiling; Zhu, Baohua; Dai, Zhong; Qin, Dongyun; Zhang, Xiaoyan; Fu, Hui] Guangdong Med Coll, Sino Amer Canc Res Inst, Dongguan 523808, Peoples R China.;[Wang, Hongqiang; Yang, Huiling] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Fang, Weiyi] Southern Med Univ, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China.;[Tian, Lu] Guangdong Pharmaceut Univ, Inst Mat Med, Guangzhou 510006, Guangdong, Peoples R China.
通讯机构：
[Fang, Weiyi] S;Southern Med Univ, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China.
关键词：
Cell proliferation;Deuterium-depleted water (DDW);NADPH:quinone oxidoreductase-1 (NQO1);Nasopharyngeal carcinoma cell (NPC);Preosteoblast cell
摘要：
Recent studies have demonstrated that natural water that has 65% of the deuterium concentration depleted, can exhibit anti-tumor properties. However, the anti-tumor effects of DDW on various nasopharyngeal carcinoma (NPC) cells have not previously been reported. In the present study, NPC cell lines and normal preosteoblast MC3T3-E1 cells were grown in RPMI1640 media containing different deuterium concentrations (50-150. ppm). The effects of DDW on the proliferation and migration of NPC and MC3T3-E1 cells were investigated using the MTT, plate colony formation, and Transwell assays, as well as Boyden chamber arrays, flow cytometry (FCM), western blot and immunofluorescence. We found that DDW was an effective inhibitor of NPC cell proliferation, plated colony formation, migration and invasion. In contrast, the growth of normal preosteoblast MC3T3-E1 cells was promoted when they were cultured in the presence of DDW. Cell cycle analysis revealed that DDW caused cell cycle arrest in the G1/S transition, reduced the number of cells in the S phase and significantly increased the population of cells in the G1 phase in NPC cells. Western blot analysis revealed that treatment with DDW significantly increased the expression of NADPH:quinone oxidoreductase-1 (NQO1), while immunofluorescence assay analysis revealed that treatment with DDW decreased the expression of PCNA and matrix metalloproteinase 9 (MMP9) in NPC cells. These results demonstrated that DDW is a novel, non-toxic adjuvant therapeutic agent that suppresses NPC cell proliferation, migration, and invasion by inducing the expression of NQO1 and causing cell cycle arrest, as well as decreasing PCNA and MMP9 expression. © 2013 Elsevier Masson SAS.
语种：
英文
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CURCUMIN TRINICOTINATE REGULATE THE PROLIFERATION OF VSMCS VIA CAVEOLIN-1/ERK1/2 SIGNALING PATHWAY
作者：
Yang, W.;Li, T. P.;Tuo, Q. H.;Zheng, X.;Guo, Z. F.;...
期刊：
CARDIOLOGY ,2013年125(SUPPL-2):465-465 ISSN：0008-6312
作者机构：
[Yang, W.; Zheng, X.; Liao, D. F.; Li, T. P.] Univ South China, Dept Pharm & Pharmacol, Hengyang, Hunan, Peoples R China.;[Guo, Z. F.; Tuo, Q. H.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.
语种：
英文
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MicroRNA-98 sensitizes cisplatin-resistant human lung adenocarcinoma cells by up-regulation of HMGA2
作者：
Xiang, Qiong;Tang, Huifang;Yu, Jia;Yin, Jie;Yang, Xiaoyan;...
期刊：
PHARMAZIE ,2013年68(4):274-281 ISSN：0031-7144
通讯作者：
Lei, Xiaoyong
作者机构：
[Tang, Huifang] Univ South China, Affilitated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Lei, Xiaoyong] Univ South China, Inst Pharm & Pharmacol, 28 Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Lei, Xiaoyong] U;Univ South China, Inst Pharm & Pharmacol, 28 Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
This study was done to explore the role of microRNA-98 (miR-98) in cisplatin sensitization in human lung adenocarcinoma cell line. Differential expressions of miRNAs were analysed between cisplatin-resistant human lung adenocarcinoma cell line A549/DDP and its parental cell A549 by miRNAs microarray, of which 14 miRNAs were showed to be significantly (> 2-fold) up-regulated and 8 miRNAs had marked down-regulation (< 0.5-fold) in A549/DDP cells compared with in A549 cells. MiR-98, a member in the let-7 family, acts as a negative regulator in the expression of HMGA2 (high mobility group A2) oncogene, and it has been shown to have a nearly 3-fold decrease in A549/DDP cells. We found that elevated expression of miR-98 led to a higher sensitivity of A549/DDP cells to cisplatin, and the protein level of HMGA2, was clearly up-regulated in both A549/DDP and A549 cells by miR-98. Moreover, both Bcl-XL and Bcl-2, were down-regulated in the Pre-miR-98(TM) transfectants cells. We for the first time demonstrated that the expression of miR-98 increases cells spontaneous apoptosis and sensitizes cells to cisplatin at least in part via HMGA2 up-regulation. Our findings provided insight into some specific miRNAs in lung cancer as potential therapeutic targets.
语种：
英文
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Statin Treatment Improves Plasma Lipid Levels but not HDL Subclass Distribution in Patients Undergoing Percutaneous Coronary Intervention
作者：
Tian, Li;Chen, Yucheng;Li, Chuanwei;Zeng, Zhi;Xu, Yanhua;...
期刊：
Lipids ,2013年48(2):127-137 ISSN：0024-4201
通讯作者：
Fu, Mingde
作者机构：
[Tian, Li; Fu, Mingde] Sichuan Univ, W China Hosp, Lab Endocrinol & Metab, Chengdu 610041, Sichuan, Peoples R China.;[Tian, Li; Fu, Mingde] Sichuan Univ, State Key Lab Biotherapy, Chengdu 610041, Peoples R China.;[Zeng, Zhi; Chen, Yucheng; Li, Chuanwei] Sichuan Univ, W China Hosp, Cardiovasc Dept, Chengdu 610041, Sichuan, Peoples R China.;[Xu, Yanhua] Chengdu Hoist Biotechnol Co Ltd, Chengdu, Sichuan, Peoples R China.;[Long, Shiyin] Univ S China, Dept Biochem & Mol Biol, Hengyang, Hunan, Peoples R China.
通讯机构：
[Fu, Mingde] S;Sichuan Univ, State Key Lab Biotherapy, Room 902,New Bldg 6,16 Sect 3,People Rd S, Chengdu 610041, Peoples R China.
关键词：
Coronary heart disease;High-density lipoprotein subclasses;Statins therapy;Quantitative coronary angiography;Two-dimensional gel electrophoresis-immunodetection
摘要：
Despite the established efficacy of statin therapy, the risk of cardiovascular events remains high in many patients. We examined high-density lipoprotein (HDL) subclass distribution profiles among statin-treated coronary heart disease (CHD) patients undergoing percutaneous coronary intervention (PCI). Plasma HDL subclasses were measured in 85 patients with established CHD and quantified by two-dimensional gel electrophoresis and immunoblotting. In CHD patients with statin treatment, the mean value of total cholesterol (TC) reached the desirable level and the triacylglycerol level (TAG) was borderline high. Moreover, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoproteinA-I, and apolipoproteinB-100 levels in these patients resembled those in normolipidemic healthy subjects. The HDL subclass did not show a normal distribution and was characterized by the lower large-sized HDL2b contents and higher contents of small-sized preβ1-HDL in CHD patients, compared to those in normolipidemic control subjects. Multiple stepwise regression analysis revealed that the severity of coronary stenosis, determined by the Gensini Score, was significantly and independently predicted by HDL2b and HDL3b. Statin therapy was effective in modifying plasma lipids levels, but not adequate as a monotherapy to normalize the HDL subclass distribution phenotype of patients with CHD undergoing PCI. The HDL subclass distribution may aid in risk stratification, especially in patients with CHD and therapeutic LDL-C and HDL-C levels.
语种：
英文
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Caspase-3 is Involved in IFN-γ- and TNF-α-Mediated MIN6 Cells Apoptosis via NF-κB/Bcl-2 Pathway
作者：
Cao, Zhao-hui;Yin, Wei-dong;Zheng, Quan-you;Feng, Shao-long;Xu, Gui-lian* ;...
期刊：
Cell Biochemistry and Biophysics ,2013年67(3):1239-1248 ISSN：1085-9195
通讯作者：
Xu, Gui-lian
作者机构：
[Yin, Wei-dong; Feng, Shao-long; Cao, Zhao-hui] Univ South China, Sch Pharm & Life Sci, Hengyang 421001, Peoples R China.;[Xu, Gui-lian; Cao, Zhao-hui] Third Mil Med Univ, Dept Immunol, Chongqing 400038, Peoples R China.;[Zheng, Quan-you; Zhang, Ke-qin; Cao, Zhao-hui] Third Mil Med Univ, Inst Surg Res, Daping Hosp, Dept Urol, Chongqing 400042, Peoples R China.
通讯机构：
[Xu, Gui-lian] T;Third Mil Med Univ, Dept Immunol, Chongqing 400038, Peoples R China.
关键词：
MIN6 cells;Caspase-3;NF-kappa B;Bcl-2;Apoptosis
摘要：
TNF-α and IFN-γ are the major pro-inflammatory cytokines in the β-cell destruction. However, the underlying mechanism remains unclear. The present study used a murine insulinoma cell line MIN6 for further investigation of the effect of Caspase-3 on the cytokines-induced pancreatic β-cell apoptosis and analyzed the mechanisms involved in the activation of Caspase-3. It was showed that the combination of IFN-γ and TNF-α significantly reduced the viability of MIN6 cells and the observed cells growth inhibition was due to cell apoptosis as judged by the morphological changes under a confocal laser scanning microscopy and FACS assay of Annexin-V/7-AAD double staining. Accompanying with NF-κB activation and Bcl-2 downregulation, both the cleaved Caspase-3 and PARP, a known substrate of Caspase-3 in vivo, were observed at 24 and 12 h, respectively, after cells exposure to IFN-γ and TNF-α treatment. Pretreatment of Caspase-3 inhibitors remarkably attenuated IFN-γ- and TNF-α-induced cells apoptosis. Inhibition of NF-κB activation led to the increase in Bcl-2 expression, a significant attenuation in Caspase-3 activity, and an obvious amelioration in cells viability in IFN-γ- and TNF-α-treated MIN6 cells. Taken together, our results indicate that Caspase-3 is critical for the induction of MIN6 cells apoptosis and it's activation is further confirmed to be related to the NF-κB-mediated Bcl-2 downregulation, which may be the underlying mechanism of IFN-γ- and TNF-α-mediated MIN6 cells apoptosis. © 2013 Springer Science+Business Media New York.
语种：
英文
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Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis
作者：
Jiang, Chuanhao;Zhao, Feijun;Xiao, Jinhong;Zeng, Tiebing;Yu, Jian;...
期刊：
Clinical and Vaccine Immunology ,2013年20(10):1563-1568 ISSN：1556-6811
通讯作者：
Wu, Yimou
作者机构：
[Jiang, Chuanhao; Wu, Yimou; Zhao, Feijun; Zeng, Tiebing; Wu, Haiying; Ma, Xiaohua] Univ South China, Coll Med, Pathogen Biol Inst, Hengyang, Peoples R China.;[Xiao, Jinhong] Hunan Prov Peoples Hosp, Clin Lab, Changsha, Hunan, Peoples R China.;[Yu, Jian] Univ South China, Coll Med, Dept Expt Zool, Hengyang, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Coll Med, Pathogen Biol Inst, Hengyang, Peoples R China.
摘要：
Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and diagnosis with sensitive and specific methods is a challenging process that is important for its prevention and treatment. In the present study, we established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and a Western blot assay for human and rabbit sera. The 20-kDa recombinant protein TpF1 was detected by Western blotting performed with sera from rabbits immunized with recombinant TpF1 and infected with the T. pallidum Nichols strain and T. pallidum clinical isolates but was not detected by Western blotting with sera from uninfected rabbits. The sensitivity of the recombinant protein was determined by screening sera from individuals with primary, secondary, latent, and congenital syphilis (n = 82). The specificity of the recombinant protein was determined by screening sera from uninfected controls (n = 30) and individuals with potentially cross-reactive infections, including Lyme disease (n = 30) and leptospirosis (n = 5). The sensitivities of TpF1-based ELISAs were 93.3%, 100%, 100%, and 100% for primary, secondary, latent, and congenital syphilis, respectively, and the specificities were all 100% for sera from uninfected controls and individuals with potentially cross-reactive infections. In Western blot assays, the sensitivities and specificities of TpF1 for human sera were all 100%. The reactivities of TpF1 with syphilitic sera were proportional to the titers of the T. pallidum particle agglutination (TPPA) assay. These data indicate that the recombinant protein TpF1 is a highly immunogenic protein in human and rabbit infections and a promising marker for the screening of syphilis. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
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英文
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Antidepressant-like and anxiolytic-like effects of hydrogen sulfide in behavioral models of depression and anxiety
作者：
Chen, Wen-Lin;Xie, Bin;Zhang, Chong;Xu, Kang-Li;Niu, Ying-Ying;...
期刊：
BEHAVIOURAL PHARMACOLOGY ,2013年24(7):590-597 ISSN：0955-8810
通讯作者：
Tang, Xiao-Qing
作者机构：
[Chen, Wen-Lin; Xie, Bin; Niu, Ying-Ying; Tang, Xiao-Qing; Xu, Kang-Li; Zou, Wei; Hu, Bi; Zhang, Chong; Zhang, Ping] Univ South China, Coll Med, Inst Cognit & Nervous Syst Dis, Hengyang 421001, Hunan, Peoples R China.;[Tian, Ying] Univ South China, Coll Med, Dept Biochem, Hengyang 421001, Hunan, Peoples R China.;[Chen, Wen-Lin; Xie, Bin; Niu, Ying-Ying; Xu, Kang-Li; Zhang, Chong] Univ South China, Coll Med, Hengyang 421001, Hunan, Peoples R China.;[Tang, Xiao-Qing] Univ South China, Inst Cognit & Nervous Syst Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tang, Xiao-Qing] U;Univ South China, Inst Cognit & Nervous Syst Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
anxiety, depression, elevated plus maze, forced swimming test, hydrogen sulfide, mouse, rat, tail suspension test
摘要：
Depression is a common and debilitating mental illness and is often comorbid with anxiety disorders. Altered synaptic plasticity is considered to be an important mechanism underlying antidepressant drug action. It has been reported that hydrogen sulfide (H2S), the third gaseous transmitter, facilitates the induction of hippocampal long-term potentiation and augments synaptic neurotransmission, involved in the regulation of synaptic plasticity. The aim of this study was to clarify the antidepressant-like and anxiolytic-like effects of H2S. H2S (NaHS, 1.68 or 5.6 mg/kg, intraperitoneally, for 7 days) exerts a specific antidepressant-like effect in the forced swimming test of mice and rats and the tail suspension test of mice, and reduces the anxiety-like behaviors of both mice and rats in the elevated plus-maze test. These results reveal a unique antagonistic action of H2S in depressive-like and anxiety-like behaviors and suggest that elevating H2S signaling in the brain may represent a novel approach for the treatment of depressive and anxiety disorders. Behavioural Pharmacology 24:590597 © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.
语种：
英文
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载药果胶基纳米粒子体外HepG2肝癌细胞的靶向性研究
作者：
王彦妹;李娜梅;刘格莎;贺冬秀;唐国涛;...
期刊：
南华大学学报(自然科学版) ,2013年27(1):69-74 ISSN：1673-0062
作者机构：
南华大学药学系药物药理研究所,湖南衡阳,421001;[严奉祥; 李娜梅; 雷小勇; 喻翠云; 王彦妹; 贺冬秀; 刘格莎; 唐国涛] 南华大学
关键词：
果胶基纳米粒子;靶向给药体系
摘要：
具有良好的生物相容性且生物可降解天然多糖，一直作为药物传递体系，具有如下优点：可延长药物的生物半衰期，减轻药物的毒副作用．本实验旨在制备一种果胶基载5-氟脲嘧啶（5-Fluorouracil，5-FU）纳米粒子（P-5-FU）载药系统，探讨果胶基纳米载药体系本身带有大量的半乳糖残基这种天然靶头对人肝癌细胞HepG2的靶向效果．M，ITr法测定载药果胶基纳米粒子对HepG2和A549细胞的增殖抑制作用，MTY结果显示P-5-Fu对HepG2细胞的增殖具有明显抑制作用，呈剂量依赖性，且作用较5-Fu强；而P-5-FU对A549细胞增殖亦有明显抑制作用，呈剂量依赖性，但与5-FU相比无明显差别；高效液相色谱法（HPLC）对两种细胞对载药纳米粒子的摄取情况和靶向性进行了测定．细胞摄取结果显示HepG2细胞对P-5-FU的摄取量较5-FU明显增多，而A549细胞对P-5-FU和5-FU的摄取量没有明显的差别．半乳糖饱和ASGPR结合位点后两种细胞对P-5-Fu和5-Fu的摄取量都没有明显的差别．结果表明果胶基纳米载药粒子可特异性靶向高表达的细胞．
语种：
中文
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Identification of Different Proteins in Colon Cancer at Different Stages by Laser Capture Microdissection Combined with Quantitative Proteomics
作者：
Huang Ying;Li Guoqing;Xiao Zhefeng;Li Maoyu;Peng Fang;...
作者机构：
[Huang Ying; Li Guoqing; Xiao Zhefeng; Li Maoyu; Peng Fang; Hu Rong; Shao Meiying; Chen Xiaojuan; Li Yuanyuan; Zhan Xianquan; Chen Zhuchu] State Local Joint Engineering Laboratory for Anticancer Drugs,Hunan Engineering Laboratory for Structural BiologyDrug Design,Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha,P.R.China,410008;[Li Guoqing] Department of Biology,School of Pharmacy and Life Science,University of South China,Hengyang,Hunan,P.R.China,410008;[Li Yuanyuan] Medical College,Guangxi University of Science and Technology,Liuzhou,Guangxi,P.R.China,545005
会议名称：
第八届中国蛋白质组学大会
会议时间：
2013-9-7
会议地点：
重庆
会议主办单位：
中国生物化学与分子生物学会
会议论文集名称：
第八届中国蛋白质组学大会论文集
关键词：
colon cancer;LCM;iTRAQ
语种：
英文
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SUMO化对DAXX亚细胞定位与功能的影响
作者：
李玲;文娟;庹勤慧;廖端芳
期刊：
生理学报 ,2013年65(1):89-95 ISSN：0371-0874
作者机构：
[李玲; 文娟; 庹勤慧] 南华大学药物药理研究所;[廖端芳] 湖南中医药大学药学院
关键词：
小泛素相关修饰蛋白;死亡结构域相关蛋白;亚细胞定位;小泛素化
摘要：
死亡结构域相关蛋白(death domain-associated protein, DAXX)是一个多功能的核蛋白, 广泛分布于核仁、核质、染色质、早幼粒细胞白血病蛋白核体(PML-NBs)和胞浆等亚细胞区域, 对细胞的转录调控、凋亡及细胞周期等生物学活动发挥重要作用。SUMO化修饰(small ubiquitin-like modification)是一种重要的、高度保守的翻译后生物学修饰, 大量文献显示SUMO化修饰对DAXX的亚细胞定位及功能的调节具有重要意义。本文对此方面的研究进展做一综述。
语种：
中文
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x连锁凋亡抑制蛋白介导二苯乙烯苷抗氧化诱导的人脐静脉内皮细胞凋亡
作者：
刘英;杨滢;李娜;仇梦霞;田英;...
期刊：
中国动脉硬化杂志 ,2013年21(9):I0019-I0019 ISSN：1007-3949
作者机构：
南华大学生物技术系生物化学与分子生物学教研室,湖南衡阳421001
会议名称：
第十二次全国动脉硬化性疾病学术会议
会议时间：
2013-10-11
会议地点：
江苏苏州
会议论文集名称：
第十二次全国动脉硬化性疾病学术会议论文集
关键词：
二苯乙烯苷;人脐静脉内皮细胞;细胞凋亡;X连锁凋亡抑制蛋白
摘要：
目的 研究二苯乙烯苷(TSG)对过氧化氢(H2O2)诱导的人脐静脉内皮细胞凋亡的作用以及TSG是否通过调节X连锁凋亡抑制蛋白(XIAP)的表达抗内皮细胞凋亡.方法 预先用不同剂量TSG(1～ 100 μmol/L)处理人脐静脉内皮细胞24h,再用300 μmol/L H2O2作用细胞24h.采用MTT法检测细胞活力,Hoeschest染色及流式细胞仪检测细胞凋亡.RT-PCR及Western blot检测TSG、XIAP抑制剂Embelin作用细胞后XIAP的表达.结果 300 μmol/L H2O2作用HUVECs细胞24 h可有效降低细胞活力,诱导细胞凋亡；1 ～ 100 μmol/L TSG预处理24 h均可提高细胞存活率,抑制细胞凋亡；30 μmol/L Embelin预处理细胞6h后再用TSG预处理24h,细胞凋亡率较TSG预处理组明显上升,细胞活力显著降低.RT-PCR、Western blot结果显示H2O2处理后,细胞XIAP表达水平显著降低；与H2O2组相比,1～ 100 μmol/L TSG预处理24h后,XIAP表达升高；与TSG预处理组相比,Embelin预处理6h后再用TSG预处理24h,XIAP的表达降低.结论 TSG能抑制H2O2诱导的人脐静脉内皮细胞凋亡,其作用机制与上调XIAP的表达有关.
语种：
中文
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Foam cells in atherosclerosis
作者：
Yu, Xiao-Hua;Fu, Yu-Chang;Zhang, Da-Wei;Yin, Kai* ;Tang, Chao-Ke
期刊：
Clinica Chimica Acta ,2013年424:245-252 ISSN：0009-8981
通讯作者：
Yin, Kai
作者机构：
[Tang, Chao-Ke; Yu, Xiao-Hua] Univ South China, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.;[Tang, Chao-Ke; Yin, Kai] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.;[Fu, Yu-Chang] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA.;[Zhang, Da-Wei] Univ Alberta, Dept Pediat, Edmonton, AB T6G 2S2, Canada.;[Zhang, Da-Wei] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada.
通讯机构：
[Yin, Kai] U;Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
Foam cells;Atherosclerosis;CD36;ACAT1;ABCA1;ABCG1
摘要：
Atherosclerosis is a chronic disease characterized by the deposition of excessive cholesterol in the arterial intima. Macrophage foam cells play a critical role in the occurrence and development of atherosclerosis. The generation of these cells is associated with imbalance of cholesterol influx, esterification and efflux. CD36 and scavenger receptor class A (SR-A) are mainly responsible for uptake of lipoprotein-derived cholesterol by macrophages. Acyl coenzyme A:cholesterol acyltransferase-1 (ACAT1) and neutral cholesteryl ester hydrolase (nCEH) regulate cholesterol esterification. ATP-binding cassette transporters A1(ABCA1), ABCG1 and scavenger receptor BI (SR-BI) play crucial roles in macrophage cholesterol export. When inflow and esterification of cholesterol increase and/or its outflow decrease, the macrophages are ultimately transformed into lipid-laden foam cells, the prototypical cells in the atherosclerotic plaque. The aim of this review is to describe what is known about the mechanisms of cholesterol uptake, esterification and release in macrophages. An increased understanding of the process of macrophage foam cell formation will help to develop novel therapeutic interventions for atherosclerosis. © 2013 The Authors.
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英文
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